CN110419535A - Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress - Google Patents
Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress Download PDFInfo
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- CN110419535A CN110419535A CN201910614483.7A CN201910614483A CN110419535A CN 110419535 A CN110419535 A CN 110419535A CN 201910614483 A CN201910614483 A CN 201910614483A CN 110419535 A CN110419535 A CN 110419535A
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- Prior art keywords
- tobacco
- triclosan
- brassinosteroid
- oxidative stress
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses brassinosteroids to alleviate triclosan to the purposes of tobacco oxidative stress, it is demonstrated experimentally that brassinosteroid alleviates triclosan to tobacco oxidative stress, it is environmental-friendly, plant is not injured.
Description
Technical field
The present invention relates to phytobiology fields, are related to the new application of brassinosteroid, more particularly to brassinosteroid
Alleviate triclosan to the purposes of tobacco oxidative stress.
Background technique
Triclosan (TCS) is a kind of artificial synthesized multiduty broad spectrum antimicrobial agent, has efficient sterilizing effect, therefore,
Frequently as disinfection sanitizer for individuals such as perfumed soap, cosmetics, face cream, toothpaste, mouthwash, articles for washing, hair fixing water
Antibiotic and sterilizing processing etc. before nursing materials, medical apparatus sterilizing and textile factory.The extensive application of TCS makes it in various rings
It is widely present in the medium of border, mainly passes through sewage effluents and be discharged into water body, enter eventually into surface water, soil and underground
Water detects TCS in sewage treatment plant's Inlet and outlet water, sludge, river, river mouth and deposit.In soil and water body
The increase of TCS influences the normal growth and development of plant.It is free that a large amount of superoxide anion is generated in TCS Plant Under The Stress body
Base, such as active oxygen cause the injury of plant membrane.
Brassinosteroid (BR) is also known as brassin lactones, is a kind of natural phytohormone, and BR is widely present in the flower of plant
In the organs such as powder, seed, stem and leaf, since to substantially exceed existing 5 kinds of hormones (auxin, abscisic acid, red mould for its physiological activity
Element, the basic element of cell division, ethylene), Yi Bei is described as the world the sixth-largest parahormone.Brassinosteroid is in the growth and development process of plant
Have the function of suspend mode and sprouting, Organ Differentiation, vascular tissue development, the elongation of cell, plant very extensive, to seed
Photomorphogenesis and photosynthesis etc. influence significant.Wherein, BR mainly passes through the elongation and division for promoting plant cell, mentions
High photosynthesis efficiency.The report of adaptability there has been no BR enhancing tobacco under triclosan adverse environmental factor at present.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of brassinosteroids to alleviate triclosan to tobacco
The purposes of oxidative stress.
Technical solution of the present invention is summarized as follows:
Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress.
Advantages of the present invention:
It is demonstrated experimentally that brassinosteroid alleviates triclosan to tobacco oxidative stress, and it is environmental-friendly, plant is not injured.
Detailed description of the invention
Fig. 1 is tobacco chlorophyll content in varied situations.
Fig. 2 is tobacco H in varied situations2O2Content.
Fig. 3 is tobacco O in varied situations2 -Content.
Fig. 4 is tobacco mda content in varied situations.
In figure, WT is 1 wild-type tobacco of reference examples；WT+TCS is reference examples 2；WT+TCS+BR is embodiment 1.
Specific embodiment
The present invention by taking wild-type tobacco as an example, be in order to enable those skilled in the art to more fully understand the present invention,
But the present invention is not imposed any restrictions.
Wild-type tobacco seed, Nutrition Soil, vermiculite, perlite are commercially available.
TCS reagent (triclosan) is purchased from Tianjin Ding Guo biotech firm, purity 98%.
BR reagent (brassinosteroid) is purchased from Tianjin Ding Guo biotech firm, and purity is to analyze pure, and when use is configured to concentration
For the mother liquor of 1mg/mL, ethyl alcohol is solvent.
Polysorbas20 is purchased from Tianjin Ding Guo biotech firm.
The measurement of physical signs: chlorophyll content, H2O2Content, O2 -Content and mda content are surveyed by detection kit
It is fixed.
The present invention is further illustrated combined with specific embodiments below.
Embodiment 1
Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress, comprising the following steps:
(1) seed sprouting is carried out:
The complete and dry wild-type tobacco mature seed of picking kind skin, by seed be seeded in equipped with Nutrition Soil, vermiculite and
In the plastic tub of perlite, the volume ratio of three is 2:2:1,25 DEG C/22 DEG C round the clock, brightness 16h/8h trains in alternate greenhouse
It supports, is sprouted to wild-type tobacco seed, growth；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, the almost the same tobacco seedling of form growing way is chosen, it is dense to move into triclosan
Degree is in the soil of 20mg/kg, and every 5d pours 1 clear water, and every 2d sprays the brassinosteroid (brassinosteroid of 1 0.3mg/L
Mother liquor is diluted with water), polysorbas20 that final volume score is 0.1% is added when sprinkling as solvent, to enhance brassinosteroid
Adherency on blade is uniformly sprayed with sprayer in plant leaf tow sides, with the whole wet and nothings of blade tow sides
Liquid drips；
(3) physiological index determining:
After tobacco seedling moves into the soil containing triclosan and plays 15d, the 3rd blade is taken from top to bottom, measures chlorophyll
Content, H2O2Content, O2 -Content and mda content.
Reference examples 1:
Step (1) is the same as 1 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, the almost the same tobacco seedling of form growing way is chosen, soil is moved into and (is free of
Triclosan) in, every 5d pours 1 clear water, and every 2d sprays 1 distilled water, and the polysorbas20 that volume fraction is 0.1% is added in when sprinkling
As solvent, to enhance adherency of the distilled water on blade, uniformly sprayed with sprayer in plant leaf tow sides, with leaf
Piece tow sides all drip by wet and no liquid；
Step (3) is the same as 1 step of embodiment (3).
Reference examples 2:
Step (1) is the same as 1 step of embodiment (1)；
(2) processing of tobacco seedling:
When tobacco seedling grows to 51 heart of leaf, the almost the same tobacco seedling of form growing way is chosen, it is dense to move into triclosan
Degree is in the soil of 20mg/kg, and every 5d pours 1 clear water, and every 2d sprays 1 distilled water, and when sprinkling is added volume fraction and is
0.1% polysorbas20 is as solvent, to enhance adherency of the distilled water on blade, with sprayer in plant leaf tow sides
It uniformly sprays, with blade tow sides, all wet and no liquid is dripped；
Step (3) is the same as 1 step of embodiment (3).
Experimental result:
(1) from figure 1 it appears that compared with reference examples 1, leaf of the tobacco under triclosan (TCS) processing in reference examples 2
Chlorophyll contents are substantially reduced, and illustrate that TCS generates certain damage to Chloroplast Ultrastructure.And embodiment 1 is planted compared with reference examples 2
The intracorporal chlorophyll content of object is increased, and illustrates that brassinosteroid (BR) can alleviate the damage that TCS generates tobacco chloroplast.
(2) it can be seen that from Fig. 2,3 in TCS stress, the intracorporal active o content of 2 tobacco of reference examples significantly improves,
Excessive active oxygen causes certain injury to tobacco plant.Active o content of the embodiment 1 compared with reference examples 2 in tobacco is
It reduces, illustrates that after spraying BR, tobacco can efficiently remove the active oxygen generated in vivo, mitigate excessive active oxygen when TCS is coerced
It is injured caused by plant.
(3) figure 4, it is seen that after being coerced by TCS, the intracorporal mda content of 2 tobacco of reference examples significantly increases
Add, is to illustrate that TCS exacerbates tobacco cell rouge in reference examples 1 4.1 times of mda content in tobacco plant body in reference examples 2
The extent of peroxidation of film.The intracorporal mda content of tobacco is 2.2 times in reference examples 1 in embodiment 1, illustrates BR to film rouge mistake
Oxidation plays certain depression effect.
Claims (1)
1. brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614483.7A CN110419535A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910614483.7A CN110419535A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress |
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| Publication Number | Publication Date |
|---|---|
| CN110419535A true CN110419535A (en) | 2019-11-08 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910614483.7A Pending CN110419535A (en) | 2019-07-09 | 2019-07-09 | Brassinosteroid alleviates triclosan to the purposes of tobacco oxidative stress |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438149A (en) * | 2019-08-08 | 2019-11-12 | 天津大学 | Turn the purposes of the resistance to triclosan of LcSABP genetic tobacco |
Citations (4)
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| CN103265360A (en) * | 2013-05-20 | 2013-08-28 | 湖南省烟草公司永州市公司 | Solid-state fertilizer specially for tobacco float breeding |
| WO2014086854A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a plant growth regulator |
| CN105191942A (en) * | 2015-09-30 | 2015-12-30 | 贵州省烟草科学研究院 | Preparation for reducing accumulation of chromium in tobacco and using method of preparation |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
-
2019
- 2019-07-09 CN CN201910614483.7A patent/CN110419535A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014086854A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a plant growth regulator |
| CN103265360A (en) * | 2013-05-20 | 2013-08-28 | 湖南省烟草公司永州市公司 | Solid-state fertilizer specially for tobacco float breeding |
| CN105191942A (en) * | 2015-09-30 | 2015-12-30 | 贵州省烟草科学研究院 | Preparation for reducing accumulation of chromium in tobacco and using method of preparation |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
Non-Patent Citations (2)
| Title |
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| 王梦姣等: "油菜素内酯的生理效应及其参与残留农药降解的研究进展", 《北方园艺》 * |
| 邓天福等: "油菜素内酯提高植物抗逆性研究进展", 《广东农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110438149A (en) * | 2019-08-08 | 2019-11-12 | 天津大学 | Turn the purposes of the resistance to triclosan of LcSABP genetic tobacco |
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Application publication date: 20191108 |