KR101818667B1 - After-ripening methods of Corydalis turtschaninovii Besser seeds for reducing after-ripening period and the high germination efficiency - Google Patents

Abstract

The invention shipbuilding Corydalis (Corydalis The present invention relates to a method for inducing the germination of seeds, and more particularly, to a method for inducing germination of seeds, in particular, a method for inducing germination of seeds, comprising the steps of seeding the seeds under a temperature- The present invention relates to a method for effectively increasing the germination of hard-colored seeds, and in accordance with the present invention, it is possible to significantly reduce the loss rate of seeds in seedling processing of the pearlescent seeds, By shortening the postharvest treatment, it is possible to maximize the cultivation efficiency of the prefecture by increasing the planting time.

Description

[BACKGROUND ART] [0002] After-ripening methods of Corydalis turtschaninovii Besser seeds for reducing post-ripening period and high germination efficiency of short-

The invention shipbuilding Corydalis (Corydalis The present invention relates to a seed growing method for shortening the seedling growth period and promoting germination efficiency. More specifically, the present invention relates to a seed growing method for reducing the seedling growing time and improving the germination efficiency of seeds by using immersing treatment with gibberellin and ethephon aqueous solution, And effectively increasing the seed germination efficiency to improve the cultivation efficiency.

The corydalis genus belonging to the genus Fumariaceae (Corydalis DC.) Is also referred to as the nest pocket. About 200 species are distributed throughout the world, and there are 19 kinds of prehistoric colors that are native to Korea, and there are Joseon color, why color, a little color, white color, Tamara color, South color and baby color. Herbaceous plant is a perennial herbaceous plant that grows in a slightly humid part of the mountain and forms a short rounded tuber with a small plant height of 20 ㎝ (Korean National Culture Grandfather, http://encykorea.aks.ac.kr/).

Pseudo-genus is called Yanhu or Yuan Hu, stem and bulb are widely used as medicines, and they are edible as juveniles. In the present study, we investigated the effects of methanol extracts on the growth of MCF-7 cells in human breast cancer cells. It also has an effect on apoptosis (Oh et al., 2007, Physiology of Pathology, 21 (6): 1437-1449). In addition, the methanol extract showed excellent antibacterial activity against acne bacteria (MIC, 62.5 μg / ml), and its association with dehydrocoridins in the constituent compounds has been reported (Lee et al., 2011, 3): 17-26) It is very valuable as a natural material development material.

Corydalis, which means lyric in Greek, has a real flower shape resembling the feather of a lark, and it has a very wide variety of colors depending on the species, so it has a very high value of ornamental value. However, it is in danger of extinction due to undesirable overfishing and degradation of native habitat and decrease of population. In Korea, there have been no studies on breeding methods to secure populations. Therefore, it is necessary to establish a sustainable supply and demand basis for effective conservation and utilization of useful resource plants by studying the breeding methods of the natural color which is excellent in pharmacological efficacy and excellent in ornamental value.

Hyanghwa is a short-lived plant that blooms in early spring and enters into dormancy after short-term growth. Chrysanthemum horn has a typical spring-sweet reputation that bloomed in April and faded in May and then entered dormancy. The spring ephemerals of eight species of native ornamental plants (May, 2012, Flower Studies 20 (4): 193-199), which were inoculated in May to June, The seeds of the precious seeds harvested at appropriate times can be germinated through inoculation with immature embryos. Although the seeds have already matured in the seeds, they contain immature embryos in the inside of the seeds. They can grow by long term post hoc treatment and germination occurs when the embryos reach a certain size inside the seeds. However, there have been no researches on the seed germination of Korean pearl seeds at home and abroad.

Gibberellic acid is a plant growth regulator of plant growth regulators produced by rice bug bacterium. It is known as a growth regulator which is known to promote seed germination, promote flowering, increase seedling growth and promote the growth of fruit. Ethephon It is an ethylene-producing growth regulator which is sold as a product called 'Erell' in 1965. It is a growth regulator used for promoting the maturation and aging of plants and for promoting coloring and maturing of fruit and vegetables. In addition, it has been reported to affect various plant growth responses such as plant growth inhibition and promotion, aeration formation and root development (Abeles, 1986, Lelie et al., 2006).

However, these plant growth regulators may not be effective when the concentration is exceeded, or the effect may be lowered, and in some cases, side effects due to the treatment of plant growth regulators are emerging. Therefore, the types and concentrations of growth regulators that are effective for seed germination and growth depend on plant species, and research on this is very important.

As mentioned above, Chosun is very useful as a natural material because it has various physiological activities. However, it is a short-lived plant in the spring, and it does not germinate without seedling treatment, has a low germination rate, It has a disadvantage that it can not sow the seed obtained in the year. Thus, the present inventors constructed a breeding method using a medicinal plant, a Chinese herbaceous seed, in order to enhance the development of germination and germination efficiency of the pearlescent seed of Chosun, and then immerse the gibberellin solution and ethephon solution, We have developed a method to increase efficiency.

It is an object of the present invention to provide a seed growing method for shortening the length of laying time and improving germination efficiency of cordierose seeds.

In order to achieve the above object,

1) Corydalis turtschaninovii Besser seeds in a mixed solution of gibberellin and etetone ;

2) wet processing the seed of step 1); And

3) heat-treating the wet treated seeds in step 2) and growing the seeds.

The present invention also provides a cordieres seed produced by the method of the present invention.

More particularly, the present invention relates to a method for inducing germination of Corydalis turtschninovii Besser seeds. More particularly, the present invention relates to a method for inducing germination of seeds of Corydalis turtschaninovii Besser seeds, According to the present invention, there is provided a method for effectively increasing the germination efficiency of the pearlescent seed by post-seeding and sowing, and the present invention relates to a method for reducing seed loss rate and promoting pear development in the seedling process of the pearlescent seed, Can be significantly increased, and by shortening the postharvest treatment, the cultivation efficiency of the prefecture of Chosun can be maximized by advancing the plant growth period.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flowchart showing the washing and disinfection process of Corydalis turtschninovii Besser seed according to an embodiment of the present invention.
FIG. 2 is a flow chart illustrating a process of fabricating a wet processing vessel for post-seeding according to an embodiment of the present invention.
FIG. 3 is a graph showing the germination percentage of cordierose seeds according to the seedling conditions in the example of the present invention;
FIG. 3 (a) is a graph showing the germination rate according to the post-hoc temperature condition;
3B is a graph showing the germination rate according to the postnatal moisture condition.
FIG. 4 is a graph showing the germination rate of the cordierose seeds according to the present invention in accordance with the growth regulator treatment;
4A is a graph showing the germination rate when the gibberellin and ethephone are treated separately;
4B is a graph showing the germination ratio when gibberellin and etetone are mixed and mixed.
FIG. 5 is a flow chart showing steps of a full-grown sowing process of a cordierge seed according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

According to the present invention,

1) immersing the seed of Corydalis turtschaninovii Besser in a mixed solution of gibberellin and etetone;

2) wet processing the seed of step 1); And

3) heat-treating the wet treated seeds in step 2) and growing the seeds.

In addition, the present invention provides a cordieres seed produced by the method of the present invention.

The seed of step 1) may be first roughened before the immersion step. Specifically, sterilized water containing 1 to 2 ml of 1% Tween 20 solution was treated to remove impurities, and then dipped in 70% ethanol for 30 seconds to 1 minute to remove bacteria and fungus. Then, It is preferable to wash the seed with sterilized distilled water.

 The gibberellin in step 1) is preferably a solution at a concentration of 200 to 800 ppm (0.02 to 0.08%), and the ethephon is preferably a solution at a concentration of 10 to 20 ppm (0.001 to 0.002%), According to the embodiment, it is most preferable that the mixed solution is 800 ppm of gibberilin and 10 ppm of etetone.

The immersion of the step 1) is preferably carried out for 20 to 30 hours, more preferably 22 to 28 hours, most preferably 24 to 26 hours. If the immersion time is too short, the effect of increasing germination efficiency by gibberellin and ethephon may be reduced. Also, if the immersion time is too long, the germination efficiency is rather lowered and the immersion time becomes too long, .

The wet treatment of step 2) is characterized by the following four steps:

(I) placing sterilized cotton or tissue paper in a container capable of accommodating a dish composed of a bottom of a circular plane and a low side surface surrounding the bottom;

II) adding the amount of the sterilized water to which the cotton or poultice of step I) is sufficiently wetted, and then making it flat;

III) placing a dish consisting of the bottom of the prepared circular plane and the lower side surrounding it in the container of step II);

IV) adding 2 to 3 filter papers to the dish of step III), and sterilizing the sterilized seed;

V) covering the filter paper 2 to 3 on the seed of the container in which the teeth are finished; And

Ⅵ) adding the sterilized water to the container in which the filter paper is sufficiently wetted in the vessel of the step V), and covering the lid of the container.

The dish consisting of the bottom of the circular plane and the lower side surrounding it is preferably petri dish.

The wet treatment step is a method of keeping the moisture of the seed in a simple manner through water retention in the vessel.

The heat treatment process in the step 3) is characterized by comprising the following three steps:

A) growing the seeded seeds at a temperature of 20 to 25 캜 for 8 to 10 weeks;

B) growing the first seed after seeding in the step a) for 4 to 6 weeks at a temperature of 10 to 15 캜; And

C) seeding the second seed obtained in step b) at a temperature of 0 to 4 ° C for 8 to 10 weeks.

In a specific embodiment of the present invention, the inventors of the present invention confirmed that the germination rate of the seeds of Pseudomonas sylvestris according to the seedling method was higher than that of the seedling matured method, (See FIG. 3).

In order to shorten the maturation period and improve the germination efficiency, the seeds were dipped in gibberellin and ethephon mixed solution for 24 hours after seed sterilization, The germination efficiency was the highest at 4 weeks at 15/10 ℃ (16/8 hours) and 8 weeks at 4 ℃ for the last 8 weeks. (See FIG. 4 and FIG. 5), the method of growing the cordierose seed of the present invention can be usefully used for the preservation and material development of the prefecture of Joseon.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

It is to be understood, however, that the following examples and experimental examples are illustrative of the present invention and are not intended to limit the scope of the present invention.

Example 1: Postnatal process of Joseon pseudo-color seed

In order to investigate the optimal postharvest method and improvement conditions of germination efficiency of seeds of Corydalis turtschaninovii Besser, seed germination after seed sterilization, seeding with different temperature conditions and germination rate were compared.

The cordierose seeds used in the present invention were cultivated in Jeollabuk-do area.

<1-1> Seed disinfection process

The corded seeds of the present invention were prepared by removing foreign materials adhering to the seeds using 1 L of sterilized water to which 1 to 20 ml of 1% Tween 20 solution was added, and then immersing the washed seeds in 70% ethanol for 30 seconds, After the molds were removed, they were washed three times using sterilized water (Fig. 1).

<1-2> Wet processing vessel for seed maturing and seed dentition process

The sterilized cotton or tissue paper was put into a petri dish, and a sufficient amount of sterilized water was added to the tissue paper to make it flat. Then, the petri dish was wrapped with tissue paper or gauze and placed in a container together with three filter paper sheets. In Example <1-1>, the sterile seed of Pseudomonas sp. Then, three filter paper sheets were placed on top of the container in which the teeth were cleaned, so that water was retained (Fig. 2).

&Lt; 1-3 >

In order to investigate the germination rate according to the post-hatching condition, the hyssophelin-treated horseradish color of the example <1-2> was repeated three times with 30 lips per each treatment, and the filter paper was sown in the petri dish for 2 times, The germination rate of each mature stage was examined.

Specifically, the experimental conditions were as follows: 16 weeks at 20 ° C, 8 weeks at 15/10 ° C (16/8 hours), and finally at 4 ° C at 4, 10, 15 and 20 ° C The experimental group was divided into two groups.

As a result, as shown in Fig. 3, it was confirmed that the seeds of the cordierose seeds improved the germination rate of the seeds after the heat treatment compared to the constant temperature treatment, but the germination rate was as low as 38%, and thus it was found necessary to further increase the germination rate (FIG. In addition, there was a problem that 40 weeks' after - harvest period could not reproduce the seed harvested in the year.

In the absence of moisture in the postnatal period, it was judged that the pearlescent seeds did not germinate and the moisture plays a very important role in the postnatal process (FIG. 3b). Therefore, it is necessary to develop a new seed germination method to shorten the maturation period of seeds and smoothly supply moisture to the seeds during the postnatal period.

<Experimental Example 1> Determination of seed germination rate of Korean pearl seeds by treatment with growth regulators

In this experiment, the optimum treatment concentration of gibberellin and ethephone, which are closely related to the seed germination promotion and maturation, was investigated to shorten the maturity period and to improve the germination efficiency.

In Example 1, it was confirmed that the germination rate of the pearlescent seeds could be increased through the heat treatment. However, since the mature period was very long, that is, 40 weeks or more, in this Experimental Example 1, , And the growth regulator was used alone or in combination.

First, 200, 400 and 800 mg of Gibberirin (0.02, 0.04, 0.08%) were added to 1 L of sterilized water to prepare a Gibberirin solution of each concentration. 10, 20, (0.001, 0.002, 0.003%) was added to the solution to prepare an etetone solution. The seed solution sterilized by the method of Example < 1-1 > was then dipped in gibberellin and etetone solution for 24 hours, . At this time, in the case of mixed treatment, except for 30 ppm of ethephon which was confirmed to reduce the germination rate of the pearlescent seeds by simple treatment.

The heat-treated seeds were treated at 20 ° C for 8 weeks, 15/10 ° C (16/8 hours) for 4 weeks and finally at 4 ° C for 8 weeks. After repeating the experiment three times, the germination rate was examined.

As a result, as shown in Fig. 4a, the seeds of the pseudohalogen seeds not treated with the growth regulator started to germinate after 24 weeks of germination and the germination rate was very low as 2.1% (Fig. 4A). On the other hand, germination rate of seeds treated with 200 to 800 ppm aqueous solution of gibberellin was increased by 8.3 to 12.4 times from 17.4 to 26.1%. In addition, germination rate of 10 to 20 ppm of etheon was slightly lower than that of gibberellin, but germination rate of 3.3 to 4.3 times was increased.

Since the germination rate of the cordierose seeds was found to be suppressed at 30 ppm of ethephon, the germination rate of gibberellin 200, 400 and 800 ppm in the mixture of growth regulators was suppressed to 30 ppm, Or 20 ppm, respectively. The growth regulator treatment and the heat treatment were carried out in the same manner as in the above experimental method.

As a result, as shown in FIG. 4B, the germination ratio was improved in all the treatments compared to the control (Fig. 4B). Therefore, it was confirmed that after immersing the pearlescent seeds in a solution containing 200 to 800 ppm of gibberellin aqueous solution and 10 to 20 ppm of aqueous solution of ethephon, the seedling of the pearlescent seeds could be shortened and the germination efficiency could be improved. According to a preferred embodiment of the present invention, when the mixture of 800 ppm of gibberylin and 10 ppm of ethephon was treated, the germination rate of the cordierose seed was 43.1%, which was about 23 times better than the untreated (2.1%) .

Claims (7)

1) immersing Corydalis turtschaninovii Besser seeds in a mixed solution of gibberellin at a concentration of 400 to 800 ppm and ethephon at a concentration of 10 to 12 ppm;
2) wet processing the seed of step 1); And
3) a step of thermally treating the wet treated seeds in step 2) and growing the seeds.
delete delete The method of claim 1, wherein the immersion in step 1) is carried out for 20 to 30 hours.
The method according to claim 1, wherein the wet treatment of step 2) comprises the following steps.
(I) placing sterilized cotton or tissue paper in a container capable of accommodating a dish composed of a bottom of a circular plane and a low side surface surrounding the bottom;
II) adding the amount of the sterilized water to which the cotton or poultice of step I) is sufficiently wetted, and then making it flat;
III) placing the dish of step I) in the vessel of step II);
IV) adding 2 to 3 filter papers to the dish of step III), and sterilizing the sterilized seed;
V) covering the filter paper 2 to 3 on the seed of the container in which the teeth are finished; And
Ⅵ) adding the sterilized water to the container in which the filter paper is sufficiently wetted in the vessel of the step V), and covering the lid of the container.
[3] The method according to claim 1, wherein the heat treatment of step 3) comprises the following three steps:
A) growing the seeded seeds at a temperature of 20 to 25 캜 for 8 to 10 weeks;
B) growing the first seed after seeding in the step a) for 4 to 6 weeks at a temperature of 10 to 15 캜; And
C) seeding the second seed obtained in step b) at a temperature of 0 to 4 ° C for 8 to 10 weeks.
Claim the shipbuilding Corydalis (Corydalis prepared by the method of claim 1 turtschaninovii Besser) seeds.

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