KR101006342B1 - Cosmetic Composition for Protecting the Skin Comprising the Extract of Magnolia Sieboldii - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a natural antiseptic agent comprising Magnolia sieboldii extract. The cosmetic composition of the present invention can be effectively used as a cosmetic product which retains the repellency, less irritation to the skin, and has excellent stability, by using the Magnolia sieboldii extract instead of the conventional organic preservative.

Preservative, Magnolia sieboldii extract, Cosmetics, Magnolia sieboldii

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for protecting skin cells containing an extract of Magnolia sieboldii,

The present invention relates to a cosmetic composition containing a natural antiseptic agent comprising Magnolia sieboldii extract.

In general, the cosmetic composition contains a large amount of water and raw materials which become a nutrient source of microorganisms (carbon source, nitrogen source and various nutrients), and microorganisms are contaminated during production (primary pollution) and during use (secondary pollution) It is necessary to add an antiseptic to prevent the growth of the contaminated microorganisms because it is exposed to microorganisms continuously for a long period of time due to the inherent characteristics of cosmetics. Contamination of microorganisms in cosmetics not only alters the constituents, properties, odor and physical properties of the cosmetics, but also causes the disease to the user depending on the type of contaminated bacteria. Therefore, ingredients that can prevent the growth of microorganisms for quality control and safety of these products are used in cosmetics, which are called preservatives.

Organic preservatives commonly used in cosmetics are preservatives of paraoxybenzoic esters, phenoxyethanol and imidazolines. However, these organic preservatives do not only represent their own toxicity to microorganisms, but they are also toxic to humans. Therefore, preservatives among cosmetic ingredients are called primary irritants.

Therefore, many researchers are making efforts to remove such preservatives and to form prescriptions using antimicrobial agents with less irritation, or to form prescription drugs without antibiotics. However, conventional cosmetics without prescription are required to be used as early as possible, and even when refrigerated, there is a problem that the period of use is only up to 3 months. Therefore, development of a hypolipidemic preservative without skin irritation Research is urgent.

The present inventors have conducted extensive studies to overcome the problem of inducing skin irritation of a chemical preservative added to cosmetics. As a result, it has been found that when the extract of Magnolia sieboldii has antibacterial activity and it is mixed with cosmetics, The stability of the formulation is excellent and the composition can be usefully added to cosmetic compositions. Thus, the present invention has been completed.

Accordingly, an object of the present invention is to provide a cosmetic composition containing a natural preservative comprising Magnolia sieboldii extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention and claims.

The above object of the present invention is achieved by a cosmetic composition containing a natural preservative comprising Magnolia sieboldii extract.

Preferably, the Magnolia sieboldii extract is selected from the group consisting of (a) water, (b) an anhydrous or a lower alcohol having 1 to 4 carbon atoms, (c) acetone, (d) ethyl acetate, (e) - butylene glycol as the extraction solvent.

Preferably, the Magnolia sieboldii extract is contained in an amount of 0.001 to 10.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the cosmetic composition is a cosmetic preparation selected from cosmetic lotion, emulsion, cream, essence, gel, pack, cleansing cream and foam cleansing; Makeup cosmetics selected from foundation, mascara, eye shadow and eyeliner; Cosmetics for hair of shampoo or conditioner; And body cosmetics such as bodywashes, and the like.

The present invention provides a cosmetic composition containing a Magnolia sieboldii extract as a natural preservative. The cosmetic of the present invention contains an extract of Magnolia sieboldii as a preservative exhibiting an antibacterial effect, thereby solving the problem of skin irritation caused by a conventional preservative and exhibiting excellent antibacterial effect.

According to one aspect of the present invention, there is provided a cosmetic composition comprising a Magnolia sieboldii extract as a preservative.

The present invention relates to the use of the Magnolia cornus ( Magnolia cornus, scientific name: Magnolia sieboldii) is galip keunki trees Magnolia sieboldii and Magnolia (Magnolia sieboldii KK _OCH ) buds. It grows wild in Korea, Japan, and China, and grows in a place where the water of the valley flows. It is 7m in height. It grows with many stems from the side along with the main stem. It is ash-colored and yellowish-brown with branches and snowy hairs on young branches and winter. Leaves are alternate phyllotaxis, egg-shaped or inverted egg-shaped long oval, sharp tip of leaf, sharp edge, grayish green on back, hairs on veins. The flowers bloom in May to June with white blooms. The leaves are bloomed and run downwards. The flowers are very good. They have the feature of bowing their heads unlike other magnolia. Flowers are 7 ~ 10cm in diameter, 6 ~ 9 petals, reddish reddish and red anther flowers. Fruits are 3-4cm long oval-shaped stones and roots, and seeds hanging from the thread come out when they ripen in September. It is planted for ornamental use, and the bark is medicinally used in the field of civilian medicine.

As used herein, the term " Magnolia sieboldii extract " means an extract obtained from various organs or parts (e.g., leaves, flowers, roots, stems, branches, Shell or flower, more preferably from an egg, shell or root, most preferably from a branch.

Each of the extracts in the present invention can be obtained through a variety of methods known in the art and is preferably selected from the group consisting of (a) water, (b) an anhydrous or hydric alcohol having from 1 to 4 carbon atoms such as methanol, ethanol, Propanol, butanol, etc.), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol as extraction solvents. The preferred extraction solvent is water, ethanol, methanol, more preferably a mixture of water and ethanol, and most preferably 70% ethanol.

The suitable amount of the extraction solvent is 3 to 10 times, preferably 4 to 8 times, and most preferably 6 times the dry weight of Angelica gigas.

It is obvious to those skilled in the art that the extract of the present invention can be obtained not only by the above-mentioned extraction solvent but also by other extraction solvents, such as a Magnolia sieboldii extract exhibiting substantially the same effect.

The Magnolia sieboldii extract of the present invention is prepared by a process comprising the following steps: (a) Magnolia siege is a mixture of 3 to 10 times water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and Adding a solvent selected from the group consisting of 1,3 butylene glycol; (b) refluxing the solvent-added Magnolia sieve of step (a) and heating and extracting it for 4 to 6 hours; And (c) filtering and drying the extracted components.

According to a specific embodiment of the present invention, the above-described extraction process is carried out as follows: First, 200 g of Angelica japonica as a raw material is washed, dried and crushed into small pieces, and then 1.2 L of 70% Is extracted by heating at 70-90 [deg.] C for 5 hours in an equipped extractor and then filtered at 300 mesh. After aging at 5-10 ° C for 7-10 days, the mixture was filtered through Wattman filter paper No. 5. The filtrate was dried at 65 ° C with a rotary evaporator to give 20.85 g of dry weight.

In addition, the extract of the present invention includes not only the extract obtained by the above-mentioned extraction method, but also the extract obtained through ordinary purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The fraction is also included in the Magnolia sieboldii extract of the present invention.

The Magnolia sieboldii extract of the present invention can be prepared in powder form by an additional process such as vacuum distillation, freeze-drying or spray-drying.

In a preferred embodiment of the present invention, the content of Magnolia sieboldii extract is 0.001 to 10.0% by weight, preferably 0.1 to 1% by weight, based on the total cosmetic composition. When the content of the ginseng extract is less than 0.001% by weight, a remarkable antimicrobial effect, especially a preservative effect can not be expected. When the content of the ginseng extract is more than 10.0% by weight, skin irritation is caused and the formulation is not stable. It is not preferable.

The ingredients contained in the cosmetic composition of the present invention include an effective ingredient capable of imparting a beneficial effect to the skin, in addition to containing the Magnolia sieboldii extract as a preservative. Examples of effective ingredients that can impart useful effects to the skin include anti-aging ingredients, wrinkle-improving ingredients, whitening ingredients, moisturizing ingredients and antibacterial ingredients, and are not limited to specific ingredients. In addition to the above components, the composition may contain components conventionally used in cosmetic compositions, including conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, and carriers.

The compositions of the present invention may be prepared in any of the formulations conventionally manufactured in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto. More particularly, when the composition of the present invention is used as a cosmetic composition, it is used as a foundation cosmetic selected from lotion, emulsion, cream, essence, gel, pack, cleansing cream and foam cleansing; Makeup cosmetics selected from foundation, mascara, eye shadow and eyeliner; Cosmetics for hair of shampoo or conditioner; And body cosmetics such as bodywashes, and the like.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention is characterized in that the preservative effect and the skin irritation mitigation effect are increased as compared with the conventional cosmetic composition. Preservatives that are used in conventional cosmetics to prevent the growth of microorganisms that pollute cosmetics from the outside and prevent product changes, typically parahydroxybenzoic acid ester and imidazoline chemical preservatives, And has a problem of inducing resistant bacteria. However, since the cosmetic composition of the present invention shows a preservative effect even in a small amount in place of such an organic chemical preservative and uses a Magnolia sieboldii extract that does not cause skin troubles, skin irritation is remarkably alleviated compared to conventional cosmetics, Effect.

The cosmetic composition of the present invention exhibits excellent repellency and skin irritation-mitigating effect and is excellent in stability of formulation, and thus can be used for all kinds of cosmetic compositions.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  One : A celestial tree  Preparation of extract

200 g of raw materials were washed, dried and crushed into small pieces. Then, the mixture was placed in 1.2 L of 70% ethanol, heated at 70-90 ° C for 5 hours in a condenser equipped with a condenser, and filtered at 300 mesh do. After aging at 5-10 ° C for 7-10 days, the mixture was filtered through Wattman filter paper No. 5. The filtrate was dried with a rotary evaporator at 65 ° C to obtain a dry weight of 20.25 g.

Experimental Example  One: A celestial tree  Experiment of antibacterial activity of extract

In order to examine the antibacterial activity of the Magnolia sieboldii extract, the cosmetic compositions of Example 1-4, which contained the Magnolia sieboldii extract at various contents ratios in the composition of the following Table 1, the cosmetic compositions of Comparative Example 1 containing no Magnolia sieboldii extract, The cosmetic composition of Comparative Example 2 containing methylparaben and imidazolidinyl urethane as preservatives was prepared instead. The extract of Magnolia sieboldii was the extract prepared in Preparation Example 1 above.

ingredient Example Comparative Example One 2 3 4 One 2 Purified water To 100 To 100 To 100 To 100 To 100 To 100 glycerin 4.0 4.0 4.0 4.0 4.0 4.0 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 Propylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Polysorbate 60 1.0 1.0 1.0 1.0 1.0 1.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Wax 4.0 4.0 4.0 4.0 4.0 4.0 Macadamia nut oil 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 Spices a very small amount a very small amount a very small amount a very small amount a very small amount a very small amount A celestial tree  extract 0.1 0.5 1.0 2.0 - - Methylparaben - - - - - 0.2 Imidazolidinyl urea - - - - - 0.2

Next, the antimicrobial effect against bacteria and fungi was measured using the cosmetic.

First, cosmetics of Examples 1, 2, 3 and 4 for the bacteria and 20-30 g of the cosmetics of Comparative Examples 1 and 2 were mixed with Escherichia coli ; ATCC 8739), Pseudomonas aeruginosa (ATCC 9027) and Staphylococcus aureus (ATCC 6538) were added and mixed at an initial concentration of 10 ⁷ cfu / g per sample. 1 g of each cosmetic was taken at 1, 7, 14, 21 and 28 days intervals while culturing them in a thermostat at 30-32 ° C for 4 weeks. The measurement results are shown in Table 2 below.

Next, in the case of mold, Candida albicans ( Candida albicans ; ATCC 10231), Penicillium citrinum ; KCTC2123) and Aspergillus niger (ATCC16404) were added at a concentration of 10 ⁷ cfu / g per sample and cultured at 25 ° C in a thermostatic bath at intervals of 7 days, The results are shown in Table 2 below.

Cosmetics Bacteria (cfu / g) mold* Initial number of bacteria Day 1 Day 7 Day 14 Day 21 Day 28 Example 1 1 x 10 ⁷ 41000 500 250 <250 <250 - Example 2 1 x 10 ⁷ 45000 300 200 200 <200 - Example 3 1 x 10 ⁷ 44000 150 <100 <100 <100 - Example 4 1 x 10 ⁷ 45000 <100 <100 <100 <100 - Comparative Example 1 1 x 10 ⁷ 54000000 30000000 > 1x10 ⁸ > 1x10 ⁸ > 1x10 ⁸ +++ Comparative Example 2 1 x 10 ⁷ 40000 300 <100 <100 <100 -

&Lt; Evaluation criteria of fungus generation degree >

-: No spawning and mycelium spawning for 8 weeks and good

+: Molding on the wall or lid within 4 weeks

++: mending within 4 weeks and mold on part of the surface

+++: Mildew and mold on the entire surface within 4 weeks

As shown in the above Table 2, the cosmetic composition of Comparative Example 1 in which no preservative was used showed that molds were formed on the entire surface and the surface within 4 weeks, and bacterial bacteria were remarkably increased even after 1 day. However, the cosmetic composition containing Magnolia sieboldii extract showed a concentration-dependent antimicrobial effect on bacteria and fungi. After about 28 days, when used in an amount of 2.0%, the conventional preservative (Comparative Example 2 ) And a better buoyancy than that of the conventional preservative was obtained when it was judged as the whole culture day, and when it was used at a content of 2.0% or more, it was found that the buoyancy was much better than that of the conventional preservative.

Experimental Example  2: First skin irritation test

In order to investigate the skin irritation of the products manufactured according to the prescribed prescription in the cosmetic composition of the present invention, 50 healthy male and female subjects were divided into 5 × 20 cm portions on both arms under a certain amount 0.1 g) for 24 hours, and the skin condition was read visually after 1 hour and 24 hours. The skin irritation rate was calculated according to the following formula (1) and the results are shown in Table 3 .

<Criteria>

-: No erythema or unusual phenomenon

+ -: slightly reddish from the surroundings

+: Significantly reddened than surrounding

++: more reddened and bulging than ambient

Cosmetics Tested embroidery Judgment result Stimulus (%) ++ + + - - Example 1 50 - - One 49 0.7 Example 2 50 - One 49 0.7 Example 3 50 - - 2 48 1.4 Example 4 50 - - 2 48 1.4 Comparative Example 1 50 - 3 48 2.1 Comparative Example 2 50 - 2 3 46 4.7

As shown in the results of the skin irritation test in the above table, the degree of irritation of the cosmetics using the Magnolia sieboldii extract (Examples 1, 2, 3 and 4) was comparative Example 2 using general preservatives and Comparative Example 1 using no preservatives (%) Of the cosmetics of the present invention.

Experimental Example  3: A celestial tree  Extract-containing Cosmetic  Stability test of formulation

The stability of the cosmetic formulation according to the present invention with temperature changes was tested. The cosmetic of Example 1 containing the Magnolia sieboldii extract as a preservative, the cosmetic of Comparative Example 1 which did not contain all of the above ingredients, and the cosmetic of Comparative Example 2 containing methylparaben and imidazolidinyl urethane as an antiseptic, The container was kept in a constant temperature bath maintained at 45 ° C for 1 week, and kept in a non-transparent glass container kept at 4 ° C for 1 week in a completely shaded refrigerator, and the degree of discoloration was compared. The degree of discoloration of the product was classified into six grades shown in the following Table 4 and the results are shown in Table 5 below.

&Lt; Evaluation criteria of product discoloration > score Evaluation standard 0 No change One Slightly different 2 A little different 3 Change a little bit 4 Severely changed 5 Extremely severe

Temperature Degree of discoloration of experimental material Example 1 Comparative Example 1 Comparative Example 2 45 ° C 0 3 0 4 ℃ 0 3 0

As can be seen in Table 5, in Comparative Example 1 in which no preservative was used, the discoloration of the product slightly changed, while the cosmetic of Example 1 containing the Magnolia sieboldii extract as a preservative hardly discolored It was found that the stability was very good, similar to the cosmetic composition of Comparative Example 2 containing methylparaben and imidazolidinyl urethane as preservatives.

Hereinafter, the present invention is not limited to the use of Magnolia sieboldii extract prepared from Example 1 of the present invention.

Formulation Example  1. Flexible lotion ( skin )

Examples of formulations of the cosmetic lotion containing the Magnolia sieboldii extract of the present invention are as follows:

ingredient Content (% by weight) The Magnolia sieboldii extract of Example 1 1.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount Purified water Balance system 100

Formulation Example  2. Nourishing lotion ( Milk lotion )

Examples of formulations of nutritional lotion (milk lotion) among cosmetics containing the Magnolia sieboldii extract of the present invention are as follows:

ingredient Content (% by weight) The Magnolia sieboldii extract of Example 1 2.0 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 60 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount Purified water Balance system 100

Formulation Example  3. Nourishing Cream

Formulation examples of nutritional creams in cosmetics containing the Magnolia sieboldii extract of the present invention are as follows:

ingredient Content (% by weight) The Magnolia sieboldii extract of Example 1 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Incense, coloring a very small amount Distilled water Balance Sum 100

Formulation Example  4. Massage  cream

Among the cosmetic compositions containing the Magnolia sieboldii extract of the present invention, examples of formulation of massage cream are as follows:

ingredient Content (% by weight) The Magnolia sieboldii extract of Example 1  0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount Purified water Balance system 100

Formulation Example  5. Pack

Formulation examples of packs of cosmetics containing the Magnolia sieboldii extract of the present invention are as follows:

ingredient Content (% by weight) The Magnolia sieboldii extract of Example 1 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glycerin 4.5 Carbopol 1.0 Polyoxide 0.1 incense a very small amount Purified water Balance system 100

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (4)

A cosmetic composition comprising a natural antiseptic agent consisting of Magnolia sieboldii extract. (C) acetone, (d) ethyl acetate, (e) chloroform or (f) 1 &lt; RTI ID = , And 3-butylene glycol as an extraction solvent. [2] The cosmetic composition according to claim 1, wherein the Magnolia sieboldii extract is contained in an amount of 0.001 to 10.0% by weight based on the total weight of the cosmetic composition. [3] The cosmetic composition according to claim 1, wherein the cosmetic composition is a foundation cosmetic selected from a lotion, an emulsion, a cream, an essence, a gel, a pack, a cleansing cream and a foam cleansing; Makeup cosmetics selected from foundation, mascara, eye shadow and eyeliner; Cosmetics for hair of shampoo or conditioner; And a cosmetic for a body passenger's body. &Lt; RTI ID = 0.0 &gt; 11. &lt; / RTI &gt;