KR102227751B1 - Natural antiseptic substitute comprising extraction mixture of Schisandra chinensis, Thymus quinquecostatus and Elsholtzia splendens as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a natural preservative substitute containing an extract mixture of Schisandra, thyme, and Elsholtzia splendens as active ingredients, and a use thereof. The extract mixture of Schisandra, thyme, and Elsholtzia splendens exhibits an excellent antibacterial effect with respect to Staphlyococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, and Aspergillus brasiliensis. A cosmetic composition containing the same has excellent antibacterial power, preservative power, and conservative power. Thus, as a natural preservative which can replace a synthetic preservative, the natural preservative substitute of the present invention can be very useful to produce a cosmetic composition.

Description

Natural antiseptic substitute comprising extraction mixture of Schisandra chinensis, Thymus quinquecostatus and Elsholtzia splendens as effective component and uses thereof

The present invention relates to a natural antiseptic substitute containing an extract mixture of Schisandra chinensis, thyme and flower perfume as an active ingredient, and a use thereof.

In order to extend the shelf life of cosmetics, substances having antibacterial or antiseptic activity should be added together. In particular, cosmetics contain water-soluble and fat-soluble ingredients, and moisture and various organic substances in these products act as good nutrients for bacteria and fungi, providing an environment in which microorganisms can easily reproduce, so it is important to increase preservation. Cosmetics contaminated with microorganisms pose a risk of microbial infection and skin troubles such as skin rash and inflammation, as well as deterioration due to the deterioration of the ingredients. Therefore, most cosmetics contain preservatives for the purpose of inhibiting or killing the growth of microorganisms. In particular, the role of these preservatives in the composition of cosmetics is more important because the period of use is very long, from several months to several years due to the nature of the product.

As a basic condition of a preservative that should be considered by the characteristics of cosmetics, it should have a broad antibacterial spectrum, be easy to use and manufacture, and should be free from skin irritation. In addition, in order to be usable for general use, it must not be affected by the acidity (pH) of the product, do not deteriorate the effect of product ingredients, and be easy to handle. To meet these conditions, paraoxybenzoate esters and parabens are commonly used as preservatives commonly used in cosmetics and pharmaceuticals, and Benzalkonium Chloride and Chlorohexidine gluconic acid are used for sterilization purposes. acid), trichlorocarbanilide, and the like have been used. However, these artificial preservatives cause skin irritation, skin allergies, etc. even with a small amount of use, and the possibility of causing cancer or genetic mutation is emerging. Accordingly, there is a need for natural preservatives to solve the side effects of existing synthetic preservatives and to ensure product safety.

On the other hand, Korean Patent Publication No. 2019-0006285 discloses a'antibacterial composition containing Schisandra chinensis extract as an active ingredient', and Korean Patent No. 0874115 discloses a'cosmetic composition containing thyme extract as an active ingredient'. Are being disclosed. However, the'natural antiseptic substitutes containing the extract mixture of Schisandra chinensis, thyme, and flower fragrance oil as active ingredients and uses thereof' of the present invention have not yet been disclosed.

The present invention was derived from the above requirements, and the present inventors prepared a preservative composition comprising a mixture of Schisandra chinensis extract, thyme extract, and flower perfume extract in a weight ratio of 4:1:1, Staphylococcus Aureus ( Staphlyococcus aureus ), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Escherichia coli (Escherichia coli), Candida albicans ( Candida albicans ) and Aspergillus brasiliensis (Aspergillus brasiliensis), as well as excellent antibacterial effect. By confirming that the antiseptic effect and formulation stability are excellent when used in cosmetic compositions, etc., the present invention was completed.

In order to solve the above problems, the present invention provides a preservative composition comprising an extract mixture of Schisandra chinensis, thyme, and flower perfumery as an active ingredient.

In addition, the present invention provides a cosmetic composition comprising 0.001 to 10% by weight based on the total weight of the preservative composition.

The extract mixture of Schisandra chinensis, thyme and flower perfume of the present invention is Staphlyococcus aureus , Pseudomonas aeruginosa , Escherichia coli , Candida albicans , and It shows an excellent antibacterial effect against Aspergillus brasiliensis , and the cosmetic composition containing it has excellent antibacterial, antiseptic and preservative power, so it is very useful in the manufacture of cosmetic compositions as a natural preservative that can replace synthetic preservatives. Can be used.

In order to achieve the object of the present invention, the present invention provides a preservative composition comprising an extract mixture of Schisandra chinensis, thyme and flower perfumery as an active ingredient.

The term "preservative" used in the present invention means that it is formulated in cosmetics and used for the purpose of preventing changes in products by inhibiting the growth of microorganisms contaminating the cosmetics from the outside.

In the preservative composition of the present invention, the extraction may be water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably ethanol, but is not limited thereto.

In addition, in the composition according to an embodiment of the present invention, the extract mixture of Schisandra chinensis, thyme, and flower perfumery

1) extracting for 10 to 30 hours by adding 10 to 30 parts by weight of ethanol to 1 part by weight of dried Schizandra, thyme, and flower fragrance, respectively, and concentrating under reduced pressure;

2) freeze-drying the concentrate obtained in step 1); And

3) mixing the Schisandra chinensis extract, thyme extract, and flower perfume extract lyophilized in step 1); may be an extraction mixture prepared including,

Preferably,

1) adding 19 parts by weight of ethanol to 1 part by weight of dried Schisandra, thyme, and flower perfume, extracting for 24 hours, and concentrating under reduced pressure; And

2) freeze-drying the concentrate obtained in step 1); And

3) mixing the Schisandra chinensis extract, thyme extract, and flower perfume extract lyophilized in step 1), but is not limited thereto.

In addition, in the composition according to an embodiment of the present invention, the mixture may be a mixture of Schisandra chinensis extract, thyme extract, and flower perfume extract in a weight ratio of 1 to 10:1 to 10:1 to 10, preferably It may be a mixture mixed in a weight ratio of 5-10:1-5:1-5, more preferably a mixture mixed in a weight ratio of 3-5:0.5-1:0.5-1, and most preferably It may be a mixture mixed at a weight ratio of 4:1:1, but is not limited thereto.

In addition, in the composition according to an embodiment of the present invention, the extract mixture of Schisandra chinensis, thyme, and flower perfumery is Staphlyococcus aureus , Pseudomonas aeruginosa ), Escherichia coli , It is characterized by having antibacterial activity against any one or more of Candida albicans and Aspergillus brasiliensis.

The present invention also provides a cosmetic composition comprising 0.001 to 10% by weight based on the total weight of the preservative composition. Preferably, the cosmetic composition may contain 0.5 to 5% by weight of the preservative composition based on the total weight, and most preferably 1% by weight, but is not limited thereto.

In the cosmetic composition according to the embodiment of the present invention, the cosmetic composition is a cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nourishing Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nourishing Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and It may be prepared in any one formulation selected from the group consisting of a body cleanser, but is not limited thereto. The cosmetic composition composed of each of these formulations may contain various bases and specimens necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as carrier components Etc. can be used.

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Propellants such as hydrocarbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the cosmetic composition of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, and the like may be used.

The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobic substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreen agents, vitamins, plant extracts, etc.

Hereinafter, it will be described in detail by examples of the present invention. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Preparation Example 1. Preparation of extract mixture of Schisandra chinensis, thyme, and flower perfume

950 ml of 70% (v/v) ethanol was added to 50 g of dried Schisandra chinensis, extracted at room temperature for 24 hours, concentrated under reduced pressure, and the concentrate obtained therefrom was lyophilized. Thyme and flower perfume extracts were also prepared in the same manner as above. The extract mixture of Schisandra chinensis, thyme and flower fragrance oil of the present invention was prepared by mixing lyophilized Schisandra chinensis extract, thyme extract, and flower fragrance oil extract in a weight ratio of 4:1:1.

Example 2. Antibacterial effect of extract mixture of Schisandra chinensis, thyme, and flower fragrance

Staphylococcus aureus (Staphlyococcus aureus ATCC 6538), Pseudomonas aeruginosa ATCC 9027, and Escherichia coli (Escherichia coli ATCC 8739) were inoculated in TSB (Tryptic soy broth) medium, respectively, and Candida albi Candida albicans ATCC 10231) is inoculated in PDB (Potato dextrose broth) medium and incubated for 18 hours in an incubator at 30 to 35°C. If the OD value is 0.6 or higher at 600 nm, add a medium and correct it to 0.6, and then the culture solution Was diluted 100 times and used as a test sample. The test sample was dissolved in TSB medium for bacteria and in PDB medium for fungi, mixed well, and then the filtered solution was diluted and used. The extract mixture of Schisandra chinensis, thyme, and flower perfume of Preparation Example 1 was treated by concentration, and the four test bacteria were inoculated, respectively, and then put into a 96-well plate and cultured in an incubator for 24 to 48 hours. After incubation, the absorbance was measured at 600 nm, and the minimum inhibitory concentration (MIC) of the extract mixture of Schisandra chinensis, thyme, and flower perfumery was confirmed. As a result, as disclosed in Table 1 below, the extract mixture of Schisandra chinensis, thyme, and flower perfume exhibited excellent antibacterial effects against Staphylococcus aureus, Pseudomonas eruginosa, E. coli and Candida albicans.

MIC value measurement result of extract mixture of Schisandra chinensis, thyme and flower perfume Strain Minimum inhibitory concentration (MIC) Of Schisandra, Thyme and Flower
Extraction mixture Staphlyococcus aureus 2.0% Pseudomonas aeruginosa 2.0% Escherichia coil 2.0% Candida albicans 4.0%

Example 3. Antibacterial effect of a composition for preservatives containing an extract mixture of Schisandra chinensis, thyme, and flower perfume

As test strains, Staphlyococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739 and Candida albicans ATCC 10231 were used. The cultured bacteria were diluted with sterile physiological saline, and the fungi were diluted by adding Tween 80 at a ratio of 0.05%, and ^{a bacterial solution of 10 6} CFU/mL was prepared and used as a test sample. A composition for preservatives (hereinafter referred to as'EG-P-ETS') containing an extract mixture of Schizandra, thyme, and flower perfume prepared as disclosed in Table 2 below was used as a bacterial medium, MHB (Mueller Hinton broth) and fungi. After dilution using GPB (Glucose Phosphate broth), which is a medium for use, it was used for the test. Inoculate a drop of bacteria solution into a preservative composition containing an extract mixture of schizandra, thyme, and flower fragrance diluted by concentration and incubate at 25 to 32°C for 1 to 3 days, and then visually observe the development of the above five test strains. The MIC value was measured. Synthetic preservatives 1,2-hexanediol (1,2-hexanediol) and euro-napri (EURO-NAPRE) were used as comparative controls. As a result, the composition for preservatives containing the extract mixture of Schisandra chinensis, thyme and flower perfumery showed excellent antibacterial effect against Staphylococcus aureus, Pseudomonas erujinosa, E. coli and Candida albicans, and synthetic preservatives 1, It was found that the effect was superior to that of 2-hexanediol (1,2-hexanediol) and euro-napre (Table 3).

Composition and content of preservative composition (EG-P-ETS) Composition Content (% by weight) Purified water 42 Glycerin 5 Caprylyl Glycol 40 Pentylene Glycol 10 Extract mixture of Schisandra chinensis, thyme and flower perfumery 3

MIC value measurement result of the preservative composition (EG-P-ETS) Strain Minimum inhibitory concentration (MIC) EG-P-ETS 1,2-hexanediol EURO-NApre Staphylococcus aureus 1.0% 2.0% 1.0% Pseudomonas Erujinosa 1.0% 2.0% 1.0% Escherichia coli 0.5% 2.0% 1.0% Candida albicans 1.0% 2.0% 1.0%

Example 4. Antiseptic power of cosmetic composition containing preservative composition (EG-P-ETS)

A cosmetic composition containing the preservative composition (EG-P-ETS) of Table 2 was prepared in a lotion formulation (Table 4), a cream formulation (Table 5), and a cleanser formulation (Table 6) to inoculate a certain number of bacteria. After that, an antiseptic test was performed by measuring the number of viable cells remaining. As the test method, the ISO 11930 Preservative Challenge test test method was used. Test strains include Staphlyococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Candida albicans ATCC 10231, and Aspergillus bra. Silliensis ( Aspergillus brasiliensis ATCC 16404) was used, and bacteria were inoculated in TSB (Tryptic soy broth) medium and cultured in an incubator at 37°C for 24 hours, and yeast was inoculated in PDB (Potato dextrose broth) medium and then 25°C. Incubated for 2 days in the incubator of, Aspergillus brassiliensis was used by collecting spores using sterile physiological saline in the cultured plate medium.

^{Test bacteria (bacteria: 1×10 5} CFU/g, yeast and mold: 1×10) prepared in 20 g each of the lotion formulation (Table 4), cream formulation (Table 5), and cleanser formulation (Table 6) to be tested in a sterilized container ⁴ CFU/g) were each inoculated. After inoculation, each sample was collected and the number of viable cells was measured. Bacteria were cultured at 37°C for 24 hours and yeast and fungi were cultured in an incubator at 25°C for 3 to 5 days, and the number of strains inoculated in each sample was confirmed. Each of the inoculated formulations was then stored at room temperature, and the number of viable cells was measured by taking 1 g of each sample on the 7th, 14th, and 28th days. TSA (Tryptic soy agar), SDA (Sabouraud dextrose agar), and PDA (Potato daxtrose agar) medium are used as a medium for measuring the number of viable cells. Bacteria are incubated at 30-35℃ for 24 to 48 hours, and fungi are incubated at 25℃. Incubation for 3 to 5 days was performed to measure the number of bacteria detected on the plate medium. As a result, as disclosed in Tables 7 to 10, Staphylococcus aureus in a lotion formulation, cream formulation, and cleanser formulation prepared with a cosmetic composition containing the preservative composition of the present invention (EG-P-ETS), Both Pseudomonas erujinosa and Escherichia coli decreased by more than 99.9% on the 7th day, Candida albicans also decreased by more than 99.9%, and Aspergillus brassiliensis decreased by more than 90% on the 14th day, resulting in a preservative composition (EG-P-ETS ) Confirmed that the antiseptic effect was excellent even when applied to cosmetics of solubilized formulation and emulsified formulation.

Lotion formulation Composition Content (% by weight) Purified water 91.3 Dipropylene glycol 2.00 PEG-60 Hydrogenated Castor Oil 0.60 Xanthan gum 0.10 Butylene Glycol 3.00 Hyaluronic acid 1% 2.00 EG-P-ETS 1.00

Cream formulation Composition Content (% by weight) Purified water 66.85 Carbomer 940 0.10 glycerin 8.00 Butylene Glycol 7.00 Hyaluronic acid 1% 0.30 Beeswax 2.00 Cetearyl Alcohol, Cetearyl Glucoside 3.50 Polysorbate 60 1.20 Sorbitan stearate 0.50 Silicone oil 1.00 Sunflower seed oil 3.00 Dimethicone 5.00 Polyacrylate-13, polyisobutene,
Polysorbate 20 0.50 Arginine 0.05 EG-P-ETS 1.00

Cleanser formulation Composition Content (% by weight) Purified water 32.9 Sodium laureth sulfate 35.00 glycerin 8.00 Disodium EDTA 0.10 Disodium laureth sulfosuccinate 5.00 PEG-120 methyl glucose dioleate 1.50 Dipropylene glycol 5.00 Cocamidopropylbetaine 10.00 Sodium chloride 1.50 EG-P-ETS 1.00

N ₀ : Number of bacteria after inoculation into cosmetic formulations _, N _x : Number of bacteria after storage at room temperature for 7 days, 14 days or 28 days, NI: Number of bacteria is not measured

Example 5. Evaluation of formulation stability of a cosmetic composition containing a preservative composition (EG-P-ETS)

A stability test was performed on the cosmetic formulation containing the composition for preservatives disclosed in Tables 4 to 6. The stability test observed changes in properties (appearance, color), pH and viscosity under room temperature conditions for 14 days. After the formulation was prepared and aged for 3 days, the property (appearance, color) and the range of the pH value were set as the test criteria. As a result, it was confirmed that all cosmetic formulations containing the preservative composition (EG-P-ETS) of the present invention were stable within the test criteria for 14 days (Tables 10 to 12).

Stability of lotion formulation containing preservative composition (EG-P-ETS) Test Items standard Condition Test day 1 day 3 days 7 days 14 days Appearance Colorless transparent liquid Room temperature ○ ○ ○ ○ pH pH 7.0±1.0 ○ ○ ○ ○

No change: ○, slight change: △, severe change: ×

Stability of cream formulation containing preservative composition (EG-P-ETS) Test Items standard Condition Test day 1 day 3 days 7 days 14 days Appearance White opaque liquid Room temperature ○ ○ ○ ○ Viscosity 42,000±5,000 cps ○ ○ ○ ○ pH pH 7.5 ±1.0 ○ ○ ○ ○

No change: ○, slight change: △, severe change: ×

Stability of Cleanser Formulation Containing Preservative Composition (EG-P-ETS) Test Items standard Condition Test day 1 day 3 days 7 days 14 days Appearance Colorless and transparent
Viscous gel Room temperature ○ ○ ○ ○ Viscosity 4,500±1,000 cps ○ ○ ○ ○ pH pH 6.2 ±1.0 ○ ○ ○ ○

No change: ○, slight change: △, severe change: ×

Claims (6)

A mixture of Schisandra chinensis, thyme, and flower oil ethanol extract mixed in a weight ratio of 4:1:1, having antibacterial activity against Candida albicans and Aspergillus brasiliensis, is included as an active ingredient. Preservative composition. delete delete delete A cosmetic composition comprising 0.001 to 10% by weight of the preservative composition of claim 1 based on the total weight. The method of claim 5, wherein the cosmetic composition is cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, lotion , Milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser Cosmetic composition, characterized in that produced in any one of the selected formulation.