KR20150119530A - Antibacterial composition containing methanol extract of Thymus quinquecostatus Celak - Google Patents
Antibacterial composition containing methanol extract of Thymus quinquecostatus Celak Download PDFInfo
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- KR20150119530A KR20150119530A KR1020140044697A KR20140044697A KR20150119530A KR 20150119530 A KR20150119530 A KR 20150119530A KR 1020140044697 A KR1020140044697 A KR 1020140044697A KR 20140044697 A KR20140044697 A KR 20140044697A KR 20150119530 A KR20150119530 A KR 20150119530A
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- KR
- South Korea
- Prior art keywords
- thyme
- extract
- methanol extract
- present
- thymus
- Prior art date
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- 239000000401 methanolic extract Substances 0.000 title claims abstract description 40
- 239000000203 mixture Substances 0.000 title claims abstract description 32
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
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Abstract
Description
The present invention relates to the use of a methanol extract or a ethyl acetate fraction thereof in the form of a thyroid gland as an anti-bacterial composition.
Natural compounds of plants are derived from the phenomenon of biodiversity. Plants produce such compounds to improve their viability and competitiveness. Because of its biological activity, many plant-derived compounds are used for the treatment of human diseases or diseases. WHO studies have shown that around 70-80% of the world's population depend primarily on traditional resources based on plant resources for their primary care (Singh et al., 2012). According to WHO, infectious diseases are the leading cause of death in low-income countries. Therefore, natural products having antibacterial properties have been studied for the treatment of such infectious diseases.
Thyme (Thymus quinquecostatus Celak, TQC) is a directional herb belonging to the genus Thymus of Lamiaceae. In ancient Chinese medicine, thyme has been used for anti-inflammatory treatment due to its analgesic effect (Jia et al., 2012). Thymol, a natural monoterpene phenol derivative of cymene, has been reported as an active compound of TQC (Shin and Kim, 2004), suggesting that thymol has strong antibacterial and antifungal activity, and thymol In addition, the anti-inflammatory and antioxidant activities of the cells are already known in vivo and in cell models (Yanishlieva et al., 1999; Braga et al., 2006; Undeger et al., 2009; Tsai et al., 2011). However, in addition to the activity of thymol itself, the research on the biological activity of the extract of the thyme extract itself is insufficient.
Thus, the present inventors confirmed that the antimicrobial effect of the methanol extract of Thyme and its fraction was not affected by the content of thymol, and therefore, the thiourea methanol extract or its fractions were useful as an antibacterial composition It is expected to be available.
An object of the present invention is to provide a pharmaceutical composition having an antimicrobial activity containing a methanol extract of a thymus quinquecostatus Celak (TQC) as an active ingredient.
It is another object of the present invention to provide a method for producing a pharmaceutical composition having the above-mentioned antibacterial activity.
In addition, another object of the present invention is to provide a thymus quinquecostatus Celak, TQC) as an active ingredient. The present invention also provides a food composition containing the above-mentioned methanol extract.
In order to achieve the above object, the present invention provides a pharmaceutical composition having antimicrobial activity containing a methanol extract of Thymus quinquecostatus Celak (TQC) as an active ingredient.
In order to accomplish the above object, the present invention provides a method for preparing a pharmaceutical composition having the above-mentioned antibacterial activity.
In order to achieve the above object, the present invention provides a food composition having antimicrobial activity containing methanol extract of the thymus quinquecostatus Celak (TQC) as an active ingredient.
The methanol extract or its ethyl acetate fraction of the thymus of the present invention has an antibacterial effect on Gram-negative and Gram-positive bacteria, so that it can be usefully used as an antibacterial pharmaceutical composition or an antibacterial food composition.
BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a view showing the content of thymol in the extract of thyme.
Figure 2 is a chart showing the hexane fraction of the methanol extract of thyme and HPLC chromatogram of thymol as a control.
Hereinafter, the present invention will be described in detail.
The term "administering" as used herein is meant to provide any desired composition of the invention to a subject in any suitable manner.
The term " pharmaceutically effective amount " as used herein means an amount sufficient to treat a disease at a reasonable benefit or risk rate applicable to medical treatment, including the type of disease, severity, activity of the drug, The time of administration, the route and rate of excretion of the drug, the duration of the treatment, factors including drugs used simultaneously and other factors well known in the medical arts.
The subject is a vertebrate animal, preferably a mammal, more preferably an experimental animal such as a mouse, a rabbit, a guinea pig, a hamster, a dog or a cat, and most preferably an ape-like animal such as a chimpanzee or a gorilla.
The present invention provides a pharmaceutical composition having antimicrobial activity containing a methanol extract of Thymus quinquecostatus Celak (TQC) as an active ingredient.
The pharmaceutical composition may include a fraction obtained by further fractionating the methanol extract of the thyroglobular surface with hexane, ethyl acetate or butanol (BuOH), and most preferably contains the ethyl acetate fraction having the most excellent antibacterial effect But is not limited thereto.
The ethyl acetate fraction of the methanol extract of the thyme may contain (-) - catechin, chlorogenic acid, rutin, rosmarinic acid and thymol, but is not limited thereto Do not.
The pharmaceutical composition preferably has an antibacterial activity, more preferably has Gram-negative and gram-positive bacteria (bacteria), and Bacillus atrophaeus , Kocuria rhizophila , Micrococcus luteus , Staphylococcus epidermidis , Bacillus subtilis subsp. Spizizenii, Klebsiella pneumonia , Enterobacter cloacae , Salmonella enterica subsp. enterica and Pseudomonas Most preferably, it has antibacterial activity on aeruginosa , but is not limited thereto.
The pharmaceutical composition of the present invention may further contain one or more active ingredients showing the same or similar functions. The pharmaceutical composition of the present invention contains 0.0001 to 10% by weight, preferably 0.001 to 1% by weight of the protein, based on the total weight of the pharmaceutical composition. In addition, the pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc., in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).
The pharmaceutical composition of the present invention may be formulated into a unit dosage form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in the form of solutions, suspensions or emulsions in oils or aqueous media, or in the form of excipients, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents. The pharmaceutical composition containing the extract of Thyme extract according to the present invention can be administered orally or parenterally in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, Preferably in the form of a suspension, a lyophilized preparation, a non-aqueous injection, or an aqueous injection solution, more preferably a sterile injectable solution preparation, and may be used in a pharmaceutical composition comprising a thyme extract Starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, Water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil Can. In the case of formulation, it can be prepared by using a solvent, a dissolution aid, a buffer, an isotonic agent, a stabilizer, an antioxidant, an anhydrous agent, a suspending agent and the like commonly used in the art. As the solvent, the additive and the diluent, , Physiological saline, pH adjusting agent, albumin, sodium chloride, mannitol, injection of Ringer's gel, glucose and the like. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.
The pharmaceutical composition of the present invention may be administered orally, subcutaneously, subcutaneously or topically through the skin (transdermal administration), orally or parenterally (e.g., intravenously, subcutaneously, intraperitoneally or topically) Oral, rectal or intravenous, intramuscular, intradermal, subcutaneous, intra-uterine or intracerebroventricular injections. The dosage varies depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of disease. The oral dosage of the pharmaceutical composition of the present invention is preferably 0.001 to 100 mg / kg (body weight) per day, more preferably 0.01 to 10 mg / kg (body weight), but is not limited thereto. However, for the most favorable effect, the thyme extract of the present invention is administered at a dose of 8 micrograms per day to 5 milligrams per day, preferably 8 micrograms to 2 milligrams, more preferably 16 micrograms to 1 milligram, May be administered by injection, more preferably by intramuscular injection. The administration frequency can be administered once a day, several times a day, once a day for two days to one week, etc., but there is no limit to the number of times of administration. The dose, administration frequency and administration method are not intended to limit the scope of the present invention in any aspect.
The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable additive, wherein the pharmaceutically acceptable additives include starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, calcium hydrogen phosphate Starch glycolate, starch glycolate, starch glycolate, carnauba wax, synthetic aluminum silicate, stearic acid, magnesium stearate, aluminum stearate, sodium carboxymethylcellulose, sodium carboxymethylcellulose, sodium carboxymethylcellulose, Calcium stearate, white sugar, dextrose, sorbitol and talc may be used. The pharmaceutically acceptable additives according to the present invention are preferably included in the pharmaceutical composition in an amount of 0.1 to 90 parts by weight, but are not limited thereto.
On the other hand, the pharmaceutical composition according to the present invention is extracted from plants, so that it can be safely used for prolonged use for preventive purposes without serious toxicity and side effects.
In addition,
1) drying and pulverizing the ground part of thyme; And
2) adding methanol to the pulverized thyme of the above 1) followed by sonication to produce a methanol extract of the thymus; and 2) a method for producing a pharmaceutical composition having an antibacterial activity.
After the step 2)
a) concentrating the methanol extract of the thymus;
b) suspending the concentrated thyme extract in water and then fractionating it with ethyl acetate, but is not limited thereto.
In addition, the present invention provides a food composition having antimicrobial activity containing a methanol extract of Thymus quinquecostatus Celak (TQC) as an active ingredient.
The concentration of the thyme extract of the present invention is preferably 0.01 to 500 μM, but is not limited thereto.
The food composition is preferably a powder, a granule, a tablet, a capsule or a beverage, but is not limited thereto.
The food of the present invention can be used as it is or in combination with other food or food ingredients, and can be suitably used according to conventional methods.
There is no particular limitation on the kind of the food. Examples of the food to which the thyme extract of the present invention can be added include meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, , A drink, an alcoholic beverage, and a vitamin complex, and includes foods in a conventional sense.
The beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. The above-mentioned natural carbohydrates are sugar alcohols such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.
There are no particular restrictions on the ingredients other than the above-mentioned thyme extract as an essential ingredient that can be included in the food composition of the present invention, and it may contain various herbal medicine extracts, food-aid additives, natural carbohydrates and the like as an additional ingredient. In addition, the food-aid additive may further include food-aid additives. Examples of the food-aid additive include food-aid additives customary in the art, for example, flavoring agents, flavoring agents, coloring agents, fillers, stabilizers and the like. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above . In addition to the above, the food composition of the present invention may contain flavoring agents such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, And salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, etc. Besides natural fruit juices and fruit juice drinks and vegetable drinks These additives may be used independently or in combination. Although the ratio of such additives is not critical, 100 parts by weight of the composition of the present invention It is generally selected from the range of 0.01 to 0.1 wt.
BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art.
< Example 1> Preparation of Thyme Extract and Its Fraction
In August 2012 Mt (Jeju) 3,600 ft altitude (N33.21.34.6 / E126.27.47.5) Thyme (Thymus in quinquecostatus Celak, TQC) were collected. The top part of the thyme was air dried at room temperature, and the dried material was pulverized into fine powder. The pulverized material (22 g) was soaked in methanol (at a dry weight of 1:10 relative to methanol) for 24 hours and then sonicated three times at 55 ° C for 1 h using an ultrasonic bath (Power Sonic 520, Hwashin Co., Respectively. Thereafter, the methanol extract was filtered to obtain a methanol extract, which was concentrated using a rotary vacuum evaporator. The concentrated methanol extract (2.86 g) was suspended in water and then fractionated by the same volume of hexane, ethyl acetate (EtOAc) and n-butanol (water-saturated buOH). The remaining aqueous extract fraction was named the water soluble fraction. Each fraction was concentrated in a vacuum concentrator. The yield of each fraction was 0.55 g of hexane fraction, 27% w / w; 0.63 g of EtOAc fraction, 31% w / w; 0.22 g BuOH fraction, 11% w / w and 0.63 g water soluble fraction, 31% w / w. Then 1000 μg of thyme methanol extract and its fractions were dissolved in 1 ml of methanol or distilled water (water soluble fraction) and used for subsequent analysis.
< Experimental Example 1> Analysis of Phenol and Flavonoid Contents of Thyme Extract and Its Fraction
<1-1> Analysis of total phenol and total flavonoid content
To determine the total phenol content (TPC) and total flavonoid content (TFC) of the methanol extract of Thyme and its fractions prepared in Example 1, Hyun et al. (2013) using the Folin-Ciocalteu method. Specifically, 100 μl of MeOH extract (100 μg / ml) and fractions thereof (100 μg / ml) were mixed with 50 μl of a 2N Folin-Ciocalteu sample, incubated at room temperature for 5 minutes, and incubated in 20% sodium carbonate ). The absorbance of the mixture was measured at 725 nm using a UV-spectrophotometer (UV-1800, Shimadzu, Tokyo, Japan). The total phenolic content of the MeOH extract and its fractions was calculated as milligrams of gallic acid equivalents (GAE) per gram of export using the equation obtained from the standard gallon graph (y = 134.7x - 0.3324, R 2 = 0.9996) . The total flavonoid content was also measured by the colorimetric method described in Hyun et al., 2013. Specifically, 0.5 ml of MeOH extract (100 占 퐂 / ml) and fractions thereof (100 占 퐂 / ml) were mixed with 0.1 ml of 10% aluminum nitrate (w / v), 1 M potassium acetate 0.1 ml and 4.3 ml of 80% ethanol, and incubated at room temperature for 40 minutes. The absorbance was then measured at 415 nm compared to the standard curve for quercetin (y = 488.06x - 0.632, R 2 = 0.9995). The total flavonoid content was measured as milligrams of quercetin equivalents (QE) per gram of extract.
As a result, as shown in Table 1, the phenol was the BuOH fraction and the flavonoid was the EtOAc fraction.
(mg GAE / g) 1)
(mg QE / g) 2)
1) Mean value (mg / g) of three repeated experiments.
2) Mean value (mg / g) of three repeated experiments.
3) Each value represents mean ± SD ( p <0.05).
<1-2> HPLC Type and content of phenol compounds
HPLC analysis of the phenolic compounds in the thyme extract and its fractions was performed using an Agilent 1260 chromatograph equipped with a diode array detector (Agilent Technologies, Waldbronn, Germany). The column temperature was maintained at 40 占 폚. As a volume, a Luna 5μ C18 (2) 100A column (4.6 × 250 mm,
The phenol compounds were detected in the EtOAc fraction as follows: (-) - catechin 0.78 ± 0.0 mg / g, chlorogenic acid 12.34 ± 0.98 mg / g, rutin 21.62 ± 0.09 mg / g and rosmarinic acid 31.34 ± 3.09 mg / g. In particular, chlorogenic acid was detected only in the EtOAc fraction. In methanol extracts, (-) - catechin was found to be 2.81 ± 0.01 mg / g and rutin was detected to be 17.01 ± 0.50 mg / g.
1) Mean value (mg / g) of three repeated experiments.
2) not detectable.
<1-3> HPLC Using thymol ( thymol ) Content and concentration analysis
HPLC analysis of the thymol content in the thyme extract and its fractions was performed using an Agilent 1260 chromatograph (Agilent Technologies, Waldbronn, Germany) equipped with a diode array detector. The column temperature was maintained at 40 占 폚. Specifically, for the analysis of thymol, MeOH / H 2 O (3: 2) was used as a mobile phase at a flow rate of 0.6 ml / min using a Poroshell 120 EC-C18 column (4.6 × 100 mm, particle size 2.7 μm) And detected at 276 nm. The standard calibration curve of thymol was performed at a concentration range of 3.125-100 / / ml (y = 8.7023x + 26.929, R 2 = 0.9948). The chromatographic peaks were confirmed by comparison of the retention times of the reference standards and the retention times of the DAD spectra (200 to 400 nm). And quantified by peak integration using an external standard method.
The content of thymol in the hexane fraction was the highest at 382.0 ± 9.0 ㎍ / g, the methanol extract at 39.2 ± 5.5 ㎍ / g and the EtOAc fraction at 136.0 ± 0.0 ㎍ / g Of thymol was present (Fig. 1). In addition, the HPLC chromatogram of the hexane fraction having the highest content of thymol was confirmed (FIG. 2).
<1-4> Statistical analysis
All samples were prepared and analyzed 3 times. Data were expressed as means ± SD. Statistical analysis was performed using ANOVA. Duncan test was used to measure significant differences between groups. Differences at p < 0.05 were considered significant.
< Experimental Example 2> Identification of antimicrobial activity of thyme extract and its fraction
To confirm anti-bacterial activity, Gram-positive bacteria Bacillus atrophaeus (KACC 14742), Kocuria rhizophila (KACC 14744), Micrococcus luteus (KACC 14819), Staphylococcus epidermidis (KACC 14822) and B. subtilis subsp. Spizizenii (KACC 14741), the gram negative bacterium Klebsiella pneumonia (KACC 14816), Enterobacter cloacae (KACC11958), Salmonella enterica subsp. enterica (KACC 10769) and Pseudomonas aeruginosa (KACC 10186) was used. Analysis was performed with a two-step dilution method (Olajuyigbe and Afolayan, 2012) to determine the minimum inhibitory concentration (MIC) of the thiourea methanol extract and its fractions. Tetracycline, an antibiotic, was used as a positive control, and bacterial growth was visually determined based on the degree of culture turbidity.
1) MIC value for bacteria.
2) Ba: Bacillus atrophaeus KACC 14742, Kr: Kocuria rhizophila KACC 14744, Ml: Micrococcus luteus KACC 14819, St. Louis: Staphylococcus epidermidis KACC 14822, Bs: Bacillus subtilis subsp. Spizizenii KACC 14741, Kp Klebsiella pneumonia KACC 14816, Ec: Enterobacter cloacae KACC11958, Se: Salmonella enterica subsp. enterica KACC 10769, Pa: Pseudomonas aeruginosa KACC 10186.
As a result, as shown in Table 3, it was found that the antibacterial activity was different depending on the extract and its fractions. As above, the EtOAc fraction had better antimicrobial activity against all bacteria than the other fractions. This effect is considered to be different depending on the constitution and content of phenolic compounds and flavonoid compounds contained in the respective fractions. It is considered that the content of thymol, which is known to have antibacterial activity in the prior art, is lower than that of the hexane fraction, , The antimicrobial activity of the EtOAc fraction of the methanol extract of thyme was not due to thymol.
Based on the results of the above-described Examples and Experimental Examples, various preparation examples and preparation examples will be presented below. However, it should be apparent to those skilled in the art that these preparation examples and preparation examples are for illustrating the present invention and that the preparation of the present invention is not limited to these preparation examples and preparation examples.
< Formulation example 1> Preparation of tablets
1 mg of the thyme extract of the present invention
Corn starch 68 mg
Lactose 90 mg
40 mg of microcrystalline cellulose
Magnesium stearate 2 mg
According to the conventional preparation method of tablets, the above ingredients were added in the prescribed amounts, uniformly mixed, stirred, and then granulated. After drying, the tablets were used to produce the desired tablets each containing 1 mg of the thiourea extract of the present invention as an effective ingredient per one tablet.
< Formulation example 2> Preparation of capsule
1 mg of the thyme extract of the present invention
Corn starch 68 mg
Lactose 90 mg
40 mg of microcrystalline cellulose
Magnesium stearate 2 mg
According to a conventional method for preparing a capsule, the above ingredients are added in the prescribed proportions, mixed uniformly, and filled in an appropriate size gelatin capsule so that 1 mg of the extract of the present invention is contained per capsule. Respectively.
< Formulation example 3> Sanje Produce
1 mg of the thyme extract of the present invention
According to a conventional method for producing an acid, the above ingredients are mixed and filled in an airtight container to prepare a powder.
< Formulation example 4> Liquid Produce
1 mg of the thyme extract of the present invention
10 g per isomer
5 g mannitol
Vitamin C 50 mg
Serine 50 mg
Maintenance amount
Purified water balance
According to a conventional method for producing a liquid agent, the above components are mixed to prepare a liquid agent.
< Manufacturing example 1> Manufacturing of food
<1-1> Manufacture of flour food
0.5 to 5.0 parts by weight of the thyme extract of the present invention was added to wheat flour per 100 parts by weight of wheat flour and the mixture was used to prepare bread, cake, cookies, crackers and noodles.
<1-2> soup And juicy ( gravies )
0.1 to 5.0 parts by weight of the thyme extract of the present invention was added to soup and juice to 100 parts by weight of soup and juice to prepare health promotion meat product, soup noodle and juice.
<1-3> Ground Beef Produce
10 parts by weight of the thyme extract of the present invention was added to ground beef to 100 parts by weight of the ground beef to prepare health promotion ground beef.
<1-4> Dairy products ( dairy products )
5 to 10 parts by weight of the thyme extract of the present invention was added to milk for 100 parts by weight of milk and various dairy products such as butter and ice cream were prepared using the milk.
<1-5> Solar Produce
Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.
Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a grinder.
The thyme extract powder of the present invention was prepared.
The grains, seeds and thyme extract of the present invention prepared above were blended in the following proportions.
(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)
Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)
The thyme extract (3 parts by weight) of the present invention,
(0.5 part by weight),
(0.5 parts by weight)
< Manufacturing example 2> Manufacturing of beverages
<2-1> Health drink Produce
(5 g) of the thyme extract of the present invention was homogeneously blended with a sub ingredient such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% And then packed in small containers such as glass bottles and plastic bottles.
<2-2> Preparation of vegetable juice
5 g of the thyme extract of the present invention was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice.
<2-3> Preparation of fruit juice
1 g of the thyme extract of the present invention was added to 1,000 ml of apple or grape juice to prepare fruit juice.
Claims (6)
2) adding methanol to the pulverized thyme of the above 1) and sonicating to produce a methanol extract of the above-ground thyme.
a) concentrating the methanol extract of the thymus;
b) suspending the concentrated thyme extract in water followed by fractionation with ethyl acetate. < RTI ID = 0.0 > 8. < / RTI >
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102227751B1 (en) * | 2020-05-29 | 2021-03-15 | 주식회사 하이솔 | Natural antiseptic substitute comprising extraction mixture of Schisandra chinensis, Thymus quinquecostatus and Elsholtzia splendens as effective component and uses thereof |
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2014
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102227751B1 (en) * | 2020-05-29 | 2021-03-15 | 주식회사 하이솔 | Natural antiseptic substitute comprising extraction mixture of Schisandra chinensis, Thymus quinquecostatus and Elsholtzia splendens as effective component and uses thereof |
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