CN104860369A - Method for adsorption recovery of triclosan in sewage by using penicillium - Google Patents
Method for adsorption recovery of triclosan in sewage by using penicillium Download PDFInfo
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- CN104860369A CN104860369A CN201510193105.8A CN201510193105A CN104860369A CN 104860369 A CN104860369 A CN 104860369A CN 201510193105 A CN201510193105 A CN 201510193105A CN 104860369 A CN104860369 A CN 104860369A
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- triclosan
- penicillium
- thalline
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
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Abstract
The invention discloses a method for adsorption recovery of triclosan in sewage by using penicillium. Specifically, the method comprises the steps of: (1) culturing penicillium to obtain a penicillium culture fluid; (2) treating the penicillium culture fluid by high-pressure steam sterilization to obtain a penicillium thallus as a adsorbent; (3) adding the penicillium thallus into sewage containing triclosan, and stirring to disperse the penicillium thallus, so as to obtain a dispersion; (4) uniformly stirring the dispersion in order to fully adsorb triclosan, so as to obtain triclosan-adsorbing penicillium thallus; and (5) extracting the triclosan-adsorbing penicillium thallus by an organic solvent and conducting pressure reduction condensation to obtain an extract containing triclosan. The penicillium used in the invention has wide source, is easy to separate and grow; and the method does not need any complex operation, and is simple and easy for application. Further, in the adsorption of adsorption, the penicillium thallus can effectively prevent degradation of triclosan into other harmful substances, so as to avoid secondary pollution.
Description
Technical field
The invention belongs to sewage treatment area, relate to a kind for the treatment of process of the sewage containing triclosan, be specifically related to a kind of method utilizing Penicillium notatum to adsorb the triclosan reclaimed in sewage.
Background technology
Triclosan is a kind of high-efficiency broad spectrum antiseptic-germicide, be widely used in personal-care supplies (as soap, toothpaste, body wash etc.), daily necessities (as plastics kitchen tools, motion footgear etc.), household cleaning product (as washing composition, sterilizing agent etc.) and medical treatment product (as sterilant, medical sanitas etc.), its application is more than 30 years.
Owing to thinking that triclosan has hypotoxicity in early days by mistake, therefore people ignore and monitor it in the sewage treatment process of routine.Research shows: in the sewage treatment process of routine, triclosan can remove about 30% under the adsorption of mud, and subsequently in the process adopting sodium-hydroxide treatment mud, part triclosan can discharge from mud, causes frequently detecting triclosan in recent years in physical environment.American National geological survey office (USGS) research shows, have 85 rivers to be detected containing triclosan in 139 rivers in 30 states, the whole America, maximum concentration reaches 2300 ng/L; In the lake of Switzerland, the content of triclosan is 10-90 ng/L; In Hispanic hemipelagic sediment, the content of triclosan is 130 μ g/L; The content of the Shi Jing korneforos triclosan of China's Pearl River Delta is 345 to 1329 ng/L, and situation allows of no optimist.
Large quantity research shows in the recent period: triclosan can block carrier proteins reductase enzyme, thus hydrobiological growth in remote effect river.In addition, through the effect of the factor such as sunlight and uviolizing, the triclosan in surface water can generate the larger Dioxins of toxicity and cause the chemical substance etc. of endocrine disturbance.In the soil applying triclosan, biocenological structure and function is affected, and microorganism growth is suppressed, and the ratio of bacterium and fungi changes.Equally, triclosan is one of chlorophenols with doubtful carinogenicity, has toxic action and endocrine to Mammals.
At present, the sewage water treatment method for triclosan mainly concentrates on absorption and degraded two aspects.Wherein, degraded aspect is divided into biological degradation and chemical degradation, and the laccase that biodegradable research mainly utilizes white-rot fungi to produce and peroxidase etc. are degraded to triclosan, but white-rot fungi growth is comparatively slow, and the production cycle is longer.In recent years, investigator finds that nano zero valence iron and zinc have reduction dechlorination to chlorinated organics, has good removal effect to chlorinated organics, and therefore, someone utilizes them to carry out chemical degradation research to triclosan.But the application in process of production of such material all runs into some problems, as Nanoscale Iron less stable, be easily oxidized to the oxyhydroxide of iron; Oxyhydroxide makes Nanoscale Iron produce passivation at Nanoscale Iron surface deposition; And aging effect and magnetic etc.In order to overcome the shortcoming of himself, often need to add multiple cofactor (as Ag, Pd, Ni, Zn, vitamins B
12deng) or need under anaerobic to carry out, this just makes the operation in sewage treatment process more loaded down with trivial details.In the absorption of triclosan, usually use the sorbent materials such as mud, zeolite, montmorillonite.But the limited sorption capacity of natural zeolite, montmorillonite, needs to add chemical reagent (as cetylpyridinium bromide, tensio-active agent etc.) and (as microwave etc.) is modified under certain condition, could increase its adsorptivity.But the chemical reagent added has toxicity usually, whether can cause secondary pollution in actual applications, still have and wait to investigate.In addition, although the adsorption effect of mud is better, subsequently in sodium-hydroxide treatment process, part triclosan can discharge from mud, causes the elimination effect of triclosan to decline.
Given this, a kind of novelty, easy and simple to handle, effective method of adsorbing the triclosan reclaimed in sewage is needed badly.
Summary of the invention
For above-mentioned situation, the object of the present invention is to provide one utilize Penicillium (
penicillium) fungi to the triclosan in sewage carry out adsorb reclaim method.Utilize extremely facile Penicillium notatum in daily life, by fermentation, autoclaving, the process such as filtration obtain sorbent material, and recycling sorbent material adsorbs the triclosan in water body.Meanwhile, utilize the thalline after the low toxicity organic solvent extraction absorption such as ethanol, triclosan extract can be obtained, reach the object of triclosan resource reclaim, and can not environmental pollution be caused.
To achieve these goals, the present invention adopts following technical scheme:
Utilize Penicillium notatum to adsorb a method for the triclosan reclaimed in sewage, it comprises the steps:
(1) getting thalline or its conidiospore suspension of Penicillium notatum, join in liquid nutrient medium, is 20 ~ 30 DEG C in temperature, and rotating speed is cultivate 1 ~ 4 day in the constant-temperature table of 120 ~ 200rpm, obtains Penicillium notatum nutrient solution;
(2) adopt the Penicillium notatum nutrient solution obtained in autoclaving treatment step (1), after filtration, obtain the mould thalline as sorbent material;
(3) according to 1kg mould thalline: the ratio of 50 ~ 200L sewage, the mould thalline obtained in step (2) is joined in the sewage containing triclosan, stir and make it disperse, obtain dispersion liquid;
(4) it is 20 ~ 30 DEG C in temperature, stirring velocity is under the condition of 120 ~ 200rpm, the dispersion liquid obtained in step (3) is stirred 2 ~ 48 hours, to make the triclosan in the abundant absorption effluent of mould thalline, after filtration, obtains the mould thalline adsorbing triclosan;
(5) adopt the mould thalline of the absorption triclosan obtained in organic solvent extraction step (4), extracting solution is evaporated to dry, obtains the medicinal extract containing triclosan.
Preferably, in technique scheme, described in step (1), Penicillium notatum is selected from any one in Penicillium, preferred penicillium oxalicum (
p. oxalicum), Penicillium digitatum (
p. digitatum), Penicllium chrysogenum (
p. chrysogenum) in any one.
Preferably, in technique scheme, described in step (1), liquid nutrient medium is selected from any one in PDA liquid nutrient medium, LB liquid nutrient medium, MS liquid nutrient medium.
Preferably, in technique scheme, the temperature of cultivating described in step (1) is 28 DEG C, and rotating speed is 150rpm, and the time is 2 days.
Preferably, in technique scheme, described in step (2), autoclaving carries out in high-pressure sterilizing pot.
Preferably, in technique scheme, described in step (3), the ratio of mould thalline and sewage is 1kg:100L.
Preferably, in technique scheme, the temperature stirred described in step (4) is 28 DEG C, and rotating speed is 150rpm, and the time is 2 hours.
Preferably, in technique scheme, described in step (5), organic solvent is selected from any one in methyl alcohol, ethanol, Virahol, acetone, preferred alcohol.
Compared with prior art, tool of the present invention has the following advantages:
(1) Penicillium is the genus in Ascomycetes distributed more widely, mould wide material sources, is very easily separated from soil, air and rotten food and obtains;
(2) mould very easily grows, and without the need to any complex operations, can grow in any matrix including machine thing, and growth cycle is short;
(3) mould thalline is in the process of absorption triclosan, triclosan effectively can be prevented to be degraded to other objectionable impuritiess, avoid secondary pollution;
(4) preparation method of thalline is simple and be easy to be separated with water body, and method is easy, be easy to apply.
Accompanying drawing explanation
Fig. 1 is that the various thalline cultivated under the culture system of PDA substratum is to the adsorptive capacity schematic diagram of triclosan.
Fig. 2 be with Zulkovsky starch for the system of the substratum of carbon source under the various thalline cultivated to the adsorptive capacity schematic diagram of triclosan.
Fig. 3 is that the thalline of the mould cultivated under the culture system of the PDA substratum thalline of living and deactivation is to the adsorptive capacity schematic diagram of triclosan.
Embodiment
The present invention is further described below in conjunction with accompanying drawing and specific embodiment.Be understandable that, these embodiments do not limit the scope of the invention.
Embodiment 1: utilize penicillium oxalicum to adsorb and reclaim triclosan.
Get the conidiospore suspension of the penicillium oxalicum be located away from Herba Artemisiae annuae body, join in PDA liquid nutrient medium, in 28 DEG C of constant-temperature tables, cultivate 2 days under the rotating speed of 150rpm, then sterilizing in high-pressure sterilizing pot, filter and obtain penicillium oxalicum thalline.According to 1:100(kg/L) ratio, thalline is joined in sewage, stir make it disperse, stir under the condition of 150rpm, temperature is 28 DEG C.Adsorption time is 2h.After triclosan absorption completely, filter the thalline obtaining adsorbing triclosan.After thalline extraction using alcohol, be evaporated to dry, obtain the medicinal extract containing triclosan.
Embodiment 2: utilize Penicillium digitatum to adsorb and reclaim triclosan.
Get the thalline of the Penicillium digitatum be located away from rotten orange peel, join in LB liquid nutrient medium, in 20 DEG C of constant-temperature tables, cultivate 1 day under the rotating speed of 200rpm, then sterilizing in high-pressure sterilizing pot, filter and obtain Penicillium digitatum thalline.According to 1:50(kg/L) ratio, thalline is joined in sewage, stir make it disperse, stir under the condition of 120rpm, temperature is 20 DEG C.Adsorption time is 24h.After triclosan absorption completely, filter the thalline obtaining adsorbing triclosan.After thalline extraction using alcohol, be evaporated to dry, obtain the medicinal extract containing triclosan.
Embodiment 3: utilize Penicllium chrysogenum to adsorb and reclaim triclosan.
Take the thalline of the Penicllium chrysogenum in industrial production penicillin, join in MS liquid nutrient medium, in 30 DEG C of constant-temperature tables, cultivate 4 days under the rotating speed of 120rpm, then sterilizing in high-pressure sterilizing pot, filter and obtain Penicllium chrysogenum thalline.According to 1:200(kg/L) ratio, thalline is added in sewage, stir make it disperse, stir under the condition of 200rpm, temperature is 30 DEG C.Adsorption time is 48 h.After triclosan absorption completely, filter the thalline obtaining adsorbing triclosan.After thalline extraction using alcohol, be evaporated to dry, obtain the medicinal extract containing triclosan.
Embodiment 4: different microorganisms is to the adsorption effect comparison test of triclosan in sewage.
After penicillium oxalicum in embodiment 1 being replaced with respectively the spherical bacterium of bamboo parasitic fungus, alternaric bacteria and mango, adopt and the experimental implementation in embodiment 1, Parallel testing mentioned microorganism is to the absorption recovering effect of triclosan in sewage, and its result as shown in Figure 1.Result shows: the adsorptive capacity of mentioned microorganism to triclosan is all less than control group, only has the triclosan adsorption of penicillium oxalicum to be obviously greater than control group, and adsorption effect is remarkable, prevents triclosan from degrading simultaneously.
Embodiment 5: different microorganisms is to the adsorption effect comparison test of triclosan in sewage.
Penicillium oxalicum in embodiment 1 is replaced with bamboo parasitic fungus and alternaric bacteria respectively, again PDA substratum is replaced with Zulkovsky starch be carbon source substratum after, adopt and the experimental implementation in embodiment 1, Parallel testing mentioned microorganism is to the absorption recovering effect of triclosan in sewage, and its result as shown in Figure 2.Result shows: bamboo parasitic fungus all demonstrates more weak adsorption under different culture media condition; The adsorption of alternaric bacteria is comparatively large by the impact of type of culture medium, and adsorption effect is unstable; Penicillium oxalicum all can demonstrate good and stable adsorption effect under the substratum system that broad variety is different.
Embodiment 6: the Penicillium notatum of different existing state is to the adsorption effect comparison test of triclosan in sewage.
Adopt and the experimental implementation in embodiment 1, Parallel testing experience deactivation and without the Penicillium notatum of deactivation to the absorption recovering effect of triclosan in sewage, its result is as shown in Figure 3.Result shows: the adsorptive capacity of viable bacteria body group is less, and the triclosan adsorption experiencing the Penicillium notatum of sterilising treatment significantly increases, its reason may be that dead thalline forms inactive, the biological adsorption material that surface tissue is fixing, only with triclosan generation adsorption.And viable bacteria body has biological activity, when contacting with triclosan, for opposing triclosan is to its toxic action, triclosan is stoped to adsorb at phage surface by changing membrane passage or changing its surface protein structure etc.
Claims (10)
1. utilize Penicillium notatum to adsorb a method for the triclosan reclaimed in sewage, it comprises the steps:
1) getting thalline or its conidiospore suspension of Penicillium notatum, join in liquid nutrient medium, is 20 ~ 30 DEG C in temperature, and rotating speed is cultivate 1 ~ 4 day in the constant-temperature table of 120 ~ 200rpm, obtains Penicillium notatum nutrient solution;
2) autoclaving treatment step 1 is adopted) the middle Penicillium notatum nutrient solution obtained, obtain the mould thalline as sorbent material after filtration;
3) according to 1kg mould thalline: the ratio of 50 ~ 200L sewage, by step 2) in obtain mould thalline join in the sewage containing triclosan, stir make it disperse, obtain dispersion liquid;
4) be 20 ~ 30 DEG C in temperature, stirring velocity is under the condition of 120 ~ 200rpm, is stirred 2 ~ 48 hours the dispersion liquid obtained in step 3), obtains the mould thalline adsorbing triclosan after filtration;
5) adopt organic solvent extraction step 4) in the mould thalline of absorption triclosan that obtains, extracting solution is evaporated to dry, obtains the medicinal extract containing triclosan.
2. method according to claim 1, is characterized in that, described in step 1), Penicillium notatum is selected from any one in Penicillium.
3. method according to claim 2, is characterized in that, Penicillium notatum described in step 1) be selected from penicillium oxalicum, Penicillium digitatum, Penicllium chrysogenum any one.
4. method according to claim 1, is characterized in that, liquid nutrient medium described in step 1) be selected from PDA liquid nutrient medium, LB liquid nutrient medium, MS liquid nutrient medium any one.
5. method according to claim 1, is characterized in that, the temperature of cultivating described in step 1) is 28 DEG C, and rotating speed is 150rpm, and the time is 2 days.
6. method according to claim 1, is characterized in that, step 2) described in autoclaving carry out in high-pressure sterilizing pot.
7. method according to claim 1, is characterized in that, the ratio of the thalline of mould described in step 3) and sewage is 1kg:100L.
8. method according to claim 1, is characterized in that, the temperature stirred described in step 4) is 28 DEG C, and rotating speed is 150rpm, and the time is 2 hours.
9. method according to claim 1, is characterized in that, organic solvent described in step 5) be selected from methyl alcohol, ethanol, Virahol, acetone any one.
10. method according to claim 9, is characterized in that, organic solvent described in step 5) is ethanol.
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| CN201510193105.8A CN104860369B (en) | 2015-04-22 | 2015-04-22 | A kind of method utilizing Penicillium notatum absorption to reclaim the triclosan in sewage |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105969760A (en) * | 2016-06-29 | 2016-09-28 | 华南理工大学 | Embedded nano-iron/single-microorganism composite microbial agent and preparation method thereof |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
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2015
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969760A (en) * | 2016-06-29 | 2016-09-28 | 华南理工大学 | Embedded nano-iron/single-microorganism composite microbial agent and preparation method thereof |
| CN105969760B (en) * | 2016-06-29 | 2020-02-18 | 华南理工大学 | Embedded nano iron/single microorganism composite microbial agent and preparation method thereof |
| CN109041975A (en) * | 2018-09-10 | 2018-12-21 | 天津大学 | A method of alleviating triclosan using salicylic acid and plant stress is injured |
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| CN104860369B (en) | 2016-09-07 |
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