CN107986454B - Method for degrading malachite green wastewater by using loofah sac loaded saccharomyces cerevisiae material - Google Patents
Method for degrading malachite green wastewater by using loofah sac loaded saccharomyces cerevisiae material Download PDFInfo
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- CN107986454B CN107986454B CN201711256381.XA CN201711256381A CN107986454B CN 107986454 B CN107986454 B CN 107986454B CN 201711256381 A CN201711256381 A CN 201711256381A CN 107986454 B CN107986454 B CN 107986454B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Abstract
The invention discloses a method for degrading malachite green wastewater by utilizing a loofah capsule loaded saccharomyces cerevisiae material. The method comprises the following steps: (1) culturing saccharomyces cerevisiae spores; (2) selecting and processing loofah capsules; (3) preparing and culturing a loofah sac-loaded saccharomyces cerevisiae material; (4) loading a saccharomyces cerevisiae material on the loofah capsules for treating malachite green dye wastewater; (5) and (4) recovering and reusing the saccharomyces cerevisiae material loaded by the loofah capsules. The material of the prepared luffa capsule loaded with the saccharomyces cerevisiae is green and economical, can realize the efficient degradation of malachite green, has the advantages of easiness in realization of solid-liquid separation and efficient recycling, and has huge application potential in the actual treatment of malachite green wastewater.
Description
Technical Field
The invention belongs to the technical field of biological wastewater treatment in the field of environmental protection and microbial immobilization, and particularly relates to a method for degrading malachite green wastewater by using a loofah sac loaded saccharomyces cerevisiae material.
Background
With the rapid development of economy, industries such as textile, printing and dyeing, leather, paper making and the like rise rapidly, and the industries bring about economic soaring and also bring serious pollution to the natural environment which we rely on to live. Malachite green is a synthetic organic dye and is widely used in textile, printing and dyeing and other industries, and a large amount of residual malachite green pollutants enter the environment along with factory wastewater to pollute water and land. Worse still, malachite green molecules containing chlorine and having a ring are inhibitors of most microorganisms, so that the biological method for removing malachite green has certain challenges, and moreover, malachite green has the hazard of carcinogenic teratogenic mutation and once entering the natural environment, the malachite green has irreversible influence on the normal production and life of human beings. Therefore, effective measures must be taken immediately to prevent and treat water pollution, soil pollution and the like caused by malachite green dye.
The commonly used methods for removing the malachite green dye wastewater mainly comprise physical methods and chemical methods, such as mesoporous materials, activated carbon adsorption, flocculating agents flocculation, membrane/electrode integrated treatment, photocatalytic degradation and the like. The biological method is widely concerned in wastewater treatment engineering due to the characteristics of environmental friendliness and low treatment cost, but because the malachite green has inhibitive performance on most microorganisms, a microbial flora with a specific degradation effect on malachite green dye is screened when the biological method is used for treating the malachite green wastewater.
The microbial microzyme of the fungus genus can resist adverse environment and is easy to culture, and is a dominant biological strain for degrading malachite green dye wastewater. However, the yeast which is easy to culture and has high propagation speed is easy to generate the problem of difficult solid-liquid separation in the wastewater treatment process, and is not beneficial to the long-term operation of the practical engineering. In order to solve the problem of difficult solid-liquid separation in the biological treatment process, immobilization technologies are increasingly applied in the biological treatment process, and commonly used immobilization carriers are mainly divided into inorganic carriers and organic carriers. According to the invention, mature loofah capsules in an organic carrier are used as the carrier to fix the domesticated saccharomyces cerevisiae to prepare the loofah capsule loaded saccharomyces cerevisiae composite material, so that the composite material has the characteristic of efficiently degrading malachite green dye wastewater, solves the problem of difficulty in solid-liquid separation of microorganisms, is beneficial to recycling of materials, and can achieve the purpose of treating wastes with processes of wastes against one another by utilizing the waste aged loofah capsules. Therefore, the loofah sponge loaded saccharomyces cerevisiae composite material is a green, economical and environment-friendly material, and has wide application prospect in organic dye wastewater treatment engineering.
Disclosure of Invention
The invention provides a method for degrading malachite green wastewater by utilizing a loofah capsule loaded saccharomyces cerevisiae material, which is simple in preparation method and convenient to operate, and the prepared loofah capsule loaded saccharomyces cerevisiae material has the effect of efficiently degrading malachite green dye wastewater, is a green and economic biological material, and has wide application prospect.
A method for degrading malachite green wastewater by utilizing a loofah sac loaded saccharomyces cerevisiae material comprises the following steps:
step 1) culture of saccharomyces cerevisiae spores: preparing a solid culture medium to culture saccharomyces cerevisiae spores;
step 2) selection and treatment of loofah capsules: selecting mature luffa which can be used for breeding, removing seeds, drying in the sun and cutting into small pieces;
step 3), preparation and culture of the loofah capsule loaded saccharomyces cerevisiae material: preparing a liquid culture medium, adding the small loofah sponge blocks in the step 2), then selecting the saccharomyces cerevisiae spores cultured in the step 1), adding the saccharomyces cerevisiae spores into the culture medium, and culturing for 3-5 days under the conditions that the temperature is 25-30 ℃ and the pH value of the liquid culture medium is 7.0;
step 4), using the loofah sac loaded saccharomyces cerevisiae material for degrading malachite green dye wastewater: washing the loofah capsules harvested in the step 3) with clear water for 3-6 times, and adding the loofah capsules to load the saccharomyces cerevisiae material according to the amount of 3-5 small loofah capsules per 100mL of malachite green wastewater, so as to degrade malachite green dye wastewater;
step 5), recycling the loofah capsule loaded saccharomyces cerevisiae material: step 4), recovering small towel gourd sac blocks after the malachite green wastewater can reach the standard and is discharged, washing the small towel gourd sac blocks for 3-6 times by using clear water, and repeatedly using the recovered material for treating the malachite green dye wastewater according to the step 4);
preferably, the formula of the solid culture medium in the step 1) is 80-120 mg/L of yeast powder, 180-220 mg/L of peptone, 150-200 mg/L of agar and 5-20 mg/L of malachite green (gradually improved), the culture condition is 25-30 ℃, the culture time is 48-60 hours, and then the solid culture medium is stored at 4 ℃ for later use;
preferably, the aged luffa serving as a carrier of the saccharomyces cerevisiae in the step 2) is cut into small pieces with uniform sizes, wherein the specification is 1cm multiplied by 1cm to 3cm multiplied by 2cm multiplied by 1 cm;
preferably, in the step 3), the preparation and culture of the loofah sac-loaded saccharomyces cerevisiae material are carried out, and the formula of the prepared liquid culture medium is glucose: 30g/L, NaNO3:3.0g/L,K2HPO4:1.0g/L,KCl:0.5g/L,MgSO4·7H2O:0.5g/L,0.1％FeSO4·7H210mL/L of O, trace elements: 10 mL/L. Wherein the formula of the trace elements is aminoacetic acid: 1.5g/L, MnSO4·H2O:5.0g/L,CoCl2·6H2O:0.1g/L,CuSO4·5H2O:0.01g/L,KAl(SO4)2·12H2O:0.01g/L,Na2MoO4·2H2O:0.01g/L,H3BO30.01g/L, pH 7.0, 2.0 × 10 of the spore content of the picked Saccharomyces cerevisiae6～5.0×106cfu/mL, adding 3-6 pieces of nutrient solution per 100mL of small pieces of loofah capsules, and culturing for 3-5 days at 25-30 ℃ under full shaking;
preferably, the concentration of malachite green wastewater in the step 4) is 25-40 mg/L, and the cultured mature loofah capsule small pieces harvested in the step 3) are added according to the amount of 3-5 pieces per 100mL of wastewater.
Compared with the prior art, the invention has the following beneficial effects:
(1) according to the invention, aged and waste loofah capsules are used as immobilized carriers for treating malachite green wastewater, so that the problem of difficulty in solid-liquid separation of microorganisms can be solved, and the purpose of treating waste with waste can be achieved;
(2) the loofah sac-loaded saccharomyces cerevisiae material prepared by the invention has a high-efficiency degradation effect on malachite green dye;
(3) the loofah sac-loaded saccharomyces cerevisiae material prepared by the invention has a stable effect of degrading malachite green dye, can be repeatedly utilized for many times, and is an economic, effective and environment-friendly biological material;
(4) the preparation method of the luffa fixed saccharomyces cerevisiae material is simple and convenient to operate.
Drawings
FIG. 1 shows the effect of a loofah capsule loaded with saccharomyces cerevisiae on degrading malachite green dye wastewater
FIG. 2 is a graph showing the recycling effect of a material loading Saccharomyces cerevisiae from loofah capsules
Detailed Description
The invention is described in further detail below with reference to the figures and examples:
example (b):
the malachite green dye wastewater is taken from a printing and dyeing process wastewater outlet of a printing and dyeing mill, and the measured malachite green concentration is 32 mg/L. Domesticating saccharomyces cerevisiae, preparing a luffa capsule loaded saccharomyces cerevisiae material, using the prepared material for treating malachite green dye wastewater, taking an experimental group without adding materials as a control group, determining degradation effects of malachite green of the experimental group and the control group, and after the experiment is finished, recovering small luffa capsules and recycling the material to treat the malachite green dye wastewater. The method comprises the following specific steps:
(1) culturing saccharomyces cerevisiae spores: preparing a solid culture medium for culturing saccharomyces cerevisiae spores, wherein the formula of the culture medium comprises 100mg/L of yeast powder, 200mg/L of peptone, 180mg/L of agar and 5 mg/L-20 mg/L of malachite green (which are gradually increased), the pH value is 7.0, the culture condition is 28 ℃, and the culture time is 48 hours;
(2) selection and treatment of loofah capsules: selecting mature luffa which can be used for breeding, removing seeds, drying in the sun, and cutting into small pieces with the specification of 3cm × 2cm × 1 cm;
(3) preparing and culturing a loofah capsule loaded saccharomyces cerevisiae material: preparing a liquid culture medium, wherein the formula is glucose: 30g/L, NaNO3:3.0g/L,K2HPO4:1.0g/L,KCl:0.5g/L,MgSO4·7H2O:0.5g/L,0.1％FeSO4·7H210mL/L of O, trace elements: 10 mL/L. Wherein the formula of the trace elements is aminoacetic acid: 1.5g/L, MnSO4·H2O:5.0g/L,CoCl2·6H2O:0.1g/L,CuSO4·5H2O:0.01g/L,KAl(SO4)2·12H2O:0.01g/L,Na2MoO4·2H2O:0.01g/L,H3BO30.01g/L, pH 7.0, adding small pieces of Luffa cylindrica (Luffa cylindrica) in (2) 4 pieces/100 mL, and selecting Saccharomyces cerevisiae spores cultured in (1) and adding into culture medium to make Saccharomyces cerevisiae spores amount to be 2.5 × 106cfu/mL, shaking well, at a temperature of 28 ℃Culturing for 3 d;
(4) the loofah sac loads a saccharomyces cerevisiae material to degrade malachite green dye wastewater: adding 4 pieces of cultured luffa capsule loaded saccharomyces cerevisiae materials into 100mL of malachite green wastewater (no material is added in a control group), fully oscillating at 28 ℃, sampling for 3mL at 0, 1, 2, 4, 8, 12 and 15h respectively, determining absorbance of the malachite green wastewater at 619nm, calculating the concentration of malachite green in simulated wastewater at each time point to obtain the degradation effect of the malachite green, wherein the experimental result is shown in figure 1, and the result shows that the luffa capsule fixed saccharomyces cerevisiae material can achieve 100% of the degradation effect on 30mg/L malachite green dye in 12h, the degradation effect of the control group is less than 8%, and the material is not degraded after 12 h;
(5) after the malachite green dye is completely degraded in step (4), small towel gourd sacs are recovered, the small towel gourd sacs are washed for 5 times by clear water and are reused for degrading malachite green dye wastewater, the absorbance of the malachite green dye is measured at 619nm in 3mL samples in 0, 1, 2, 4, 8, 12 and 15h, the concentration of the malachite green in the wastewater at each time point is calculated to obtain the degradation effect of the malachite green dye, the experimental result is shown in figure 2, the result shows that the malachite green degradation effect is not reduced when the wine yeast material fixed by the towel gourd sacs is reused for the first time and the second time, the removal rate of the malachite green dye is 83.25% in 12h and the degradation effect of the wine yeast material for 15h can reach 90% in the third time of reuse. The loofah sac loaded saccharomyces cerevisiae material has a good degradation effect on malachite green dye wastewater, is good in material stability, and can be repeatedly utilized for many times. Therefore, the loofah sac-loaded saccharomyces cerevisiae material has great potential in the aspect of treatment of malachite green dye wastewater.
Claims (6)
1. A method for degrading malachite green wastewater by utilizing a loofah sac loaded saccharomyces cerevisiae material comprises the following steps:
step 1) culture of saccharomyces cerevisiae spores: preparing a solid culture medium to culture saccharomyces cerevisiae spores;
step 2) selection and treatment of loofah capsules: selecting mature luffa which can be used for breeding, removing seeds, drying in the sun and cutting into small pieces;
step 3), preparation and culture of the loofah capsule loaded saccharomyces cerevisiae material: preparing a liquid culture medium, adding the small loofah sponge blocks in the step 2), then selecting the saccharomyces cerevisiae spores cultured in the step 1), adding the saccharomyces cerevisiae spores into the culture medium, and culturing for 3-5 days under the conditions that the temperature is 25-30 ℃ and the pH value of the liquid culture medium is 7.0;
and 4) using the loofah sac loaded saccharomyces cerevisiae material for degrading malachite green wastewater: washing the loofah capsules harvested in the step 3) with clear water for 3-6 times, and adding the loofah capsules to load the saccharomyces cerevisiae material according to the amount of 3-5 small loofah capsules per 100mL of malachite green wastewater for degrading the malachite green wastewater;
step 5), recycling the loofah capsule loaded saccharomyces cerevisiae material: and 4) recovering small towel gourd sac blocks after the malachite green wastewater can reach the standard and is discharged, washing the small towel gourd sac blocks for 3-6 times by using clear water, and repeatedly using the recovered material for treating the malachite green wastewater according to the step 4).
2. The method for degrading malachite green wastewater by using the loofah sac loaded with the saccharomyces cerevisiae material according to claim 1, characterized in that: the formula of the solid culture medium in the step 1) is 80-120 mg/L of yeast powder, 180-220 mg/L of peptone, 150-200 mg/L of agar and 5-20 mg/L of malachite green, the concentration is gradually increased, the culture condition is 25-30 ℃, the culture time is 48-60 hours, and then the solid culture medium is stored at 4 ℃ for later use.
3. The method for degrading malachite green wastewater by using the loofah sac loaded with the saccharomyces cerevisiae material according to claim 1, characterized in that: step 2) selection and treatment of loofah capsules: aged loofah is selected as a carrier of saccharomyces cerevisiae, and the loofah capsules after seed removal and sun drying are cut into small blocks with uniform sizes, wherein the sizes are 1cm multiplied by 1cm to 3cm multiplied by 2cm multiplied by 1 cm.
4. The method for degrading malachite green wastewater by using the loofah sac loaded with the saccharomyces cerevisiae material according to claim 1, characterized in that: step 3), preparation and culture of the loofah capsule loaded saccharomyces cerevisiae material: the formula of the prepared culture medium is glucose: 30g/L, NaNO3：3.0g/L，K2HPO4：1.0g/L，KCl：0.5g/L，MgSO4·7H2O：0.5g/L，0.1％FeSO4·7H2O:10mL/L, trace elements: 10 mL/L; wherein the formula of the trace elements is aminoacetic acid: 1.5g/L, MnSO4·H2O：5.0g/L，CoCl2·6H2O：0.1g/L，CuSO4·5H2O：0.01g/L，KAl(SO4)2·12H2O：0.01g/L，Na2MoO4·2H2O：0.01g/L，H3BO3:0.01g/L, pH 7.0, and the content of spores of Saccharomyces cerevisiae is 2.0 × 106～5.0×106cfu/mL, the adding amount of the small towel gourd bag blocks is 3-6 blocks/100 mL of nutrient solution, and the culture is carried out for 3-5 days at 25-30 ℃ under the condition of full shaking.
5. The method for degrading malachite green wastewater by using the loofah sac loaded with the saccharomyces cerevisiae material according to claim 1, characterized in that: and 4) putting 3-5 pieces of mature cultured luffa capsule small pieces/100 mL of malachite green wastewater harvested in the step 3) into the malachite green wastewater with the concentration of 25-40 mg/L.
6. The method for degrading malachite green wastewater by using the loofah sac loaded with the saccharomyces cerevisiae material according to claim 1, characterized in that: and 5) after completely degrading the malachite green wastewater, filtering out small towel gourd sacs by using a filter screen, washing the small towel gourd sacs by using clear water for 3-6 times, and repeatedly utilizing the small towel gourd sacs to degrade the malachite green wastewater according to the step 4).
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