CN107986454A - A kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater - Google Patents
A kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater Download PDFInfo
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- CN107986454A CN107986454A CN201711256381.XA CN201711256381A CN107986454A CN 107986454 A CN107986454 A CN 107986454A CN 201711256381 A CN201711256381 A CN 201711256381A CN 107986454 A CN107986454 A CN 107986454A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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Abstract
The invention discloses a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater.Comprise the following steps：(1) culture of saccharomyces cerevisiae spore；(2) selection and processing of towel gourd；(3) preparation and its culture of towel gourd load saccharomyces cerevisiae material；(4) towel gourd load saccharomyces cerevisiae material is used to handle malachite green dye waste water；(5) recycling of towel gourd load saccharomyces cerevisiae material.Towel gourd load saccharomyces cerevisiae material green, economy prepared by the present invention is, it can be achieved that the advantages of efficient degradation of malachite green, is provided simultaneously with being easily achieved separation of solid and liquid, and high efficiente callback utilizes, possesses huge application potential in the actual treatment of malachite green wastewater.
Description
Technical field
The invention belongs to the biological wastewater treatment technology of field of environment protection, immobilized microorganism technology field, and in particular to one
The method that kind loads saccharomyces cerevisiae material degradation malachite green wastewater using towel gourd.
Background technology
Emerge with the rapid development of economy, the industry such as weaving, printing and dyeing, leather, papermaking is rapid, these industry bring through
While Ji rapid development, also serious pollution is brought to the natural environment that we depend on for existence.Malachite green is a kind of synthesis
Organic dyestuff, is widely used in the industry such as weave and print and dye, a large amount of remaining malachite green pollutants with plant chimney stalk into
Enter environment, polluted-water and soil.Worse, in malachite green molecule chloride and band be most of microbe suppression
Preparation, so that removing malachite green using bioanalysis has certain challenge, in addition, malachite green has carcinogenic teratogenesis
Once the normal production and living of the mankind into natural environment, will be produced irreversible influence by mutagenic harm.Cause
This, it is necessary to water pollution, soil pollution etc. caused by prevention malachite green dyestuff of adopting an effective measure at once.
The method for being commonly used to remove malachite green dye waste water is mainly Physical and chemical method, such as mesoporous material, activity
Charcoal adsorbs, flocculant flocculation, film/electrode integrated treatment and photocatalytic degradation etc., although these methods give up malachite green
Water has efficient removal efficiency, but processing cost becomes the principal element of limitation physical-chemical process processing malachite green wastewater.
And bioanalysis is environmental-friendly with its, the characteristics of processing cost is cheap, has obtained extensive concern in waste water treatment engineering, but by
All there is inhibition to most of microbe in malachite green, therefore, should when using bioanalysis to handle malachite green wastewater
Filter out the microorganism species that there is selective degradation effect to malachite green dyestuff.
Fungi microbial starter bacterium, can resist poor environment, and be easy to cultivate, and be contaminated for degrading malachite green
Expect the dominant strain of waste water.But be easy to culture and the fast saccharomycete of reproduction speed easily produced in wastewater treatment process it is solid
The problem of liquid separation is difficult, will be unfavorable for the permanent operation of Practical Project.In order to solve separation of solid and liquid during Biochemical method
The problem of difficult, utilization of the immobilization technology in biological treatment is more and more, and common fixation support is broadly divided into nothing
Airborne body and organic carrier.The present invention fixes the wine brewing after domestication by the use of the ripe towel gourd in organic carrier as carrier
Saccharomycete, prepare towel gourd load saccharomyces cerevisiae composite material, the characteristic both with efficient degradation malachite green dye waste water,
Solve the problem of microorganism separation of solid and liquid difficulty again, also help the recycling of material, discard the towel gourd of aging
Using can also achieve the purpose that the treatment of wastes with processes of wastes against one another.Therefore, towel gourd load saccharomyces cerevisiae composite material is a kind of green economy, ring
Border friendly material, will be with a wide range of applications in Organic Dyestuff Wastewater Treatment engineering.
The content of the invention
The present invention provides a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater, it is prepared
Method it is simple, easy to operate, the towel gourd for preparing load saccharomyces cerevisiae material has efficient degradation malachite green dyestuff
The effect of waste water, is a kind of biomaterial of green economy, has broad application prospects.
A kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater, comprises the following steps：
The culture of step 1) saccharomyces cerevisiae spore：Prepare solid medium culture saccharomyces cerevisiae spore；
The selection and processing of step 2) towel gourd：Growth selection maturation can be used for the sponge gourd of breeding, and remove seed is shone dry doubling and cut
Into fritter；
Step 3) towel gourd loads the preparation and its culture of saccharomyces cerevisiae material：Fluid nutrient medium is prepared, adds step 2)
In towel gourd fritter, then picking step 1) in culture saccharomyces cerevisiae spore add culture medium in, in 25~30 DEG C of temperature, liquid
3~5d is cultivated under conditions of body medium pH=7.0；
Towel gourd load saccharomyces cerevisiae material is used for degrading malachite green waste water from dyestuff by step 4)：Clear water washing step 3)
The towel gourd load saccharomyces cerevisiae material of middle harvest 3~6 times, and by 3~5 pieces of towel gourd fritter/100mL malachite green wastewaters
Amount adds towel gourd load saccharomyces cerevisiae material, for degrading malachite green waste water from dyestuff；
Step 5) towel gourd loads the recycling of saccharomyces cerevisiae material：Step 4) Malachite Green waste water can row up to standard
After putting, towel gourd fritter is recycled, is washed 3~6 times with clear water, the material of recycling is recycled and reused for malachite green dye according to step 4)
Expect the processing of waste water；
Preferably, step 1) solid culture based formulas is 80~120mg/L of dusty yeast, 180~220mg/L of peptone, fine jade
150~200mg/L of fat, malachite green concentration 5mg/L~20mg/L (stepping up), condition of culture is 25~30 DEG C, during culture
Between 48~60h, it is spare under the conditions of being then stored in 4 DEG C；
Preferably, step 2) selects carrier of the sponge gourd as saccharomyces cerevisiae of aging, the sponge gourd by remove seed and after drying
Capsule is cut into fritter of uniform size, and specification is 1cm × 1cm × 1cm~3cm × 2cm × 1cm；
Preferably, the preparation and its culture of step 3) towel gourd load saccharomyces cerevisiae material, the Liquid Culture basigamy of preparation
Side is glucose：30g/L,NaNO3:3.0g/L,K2HPO4:1.0g/L,KCl:0.5g/L,MgSO4·7H2O:0.5g/L, 0.1%
FeSO4·7H2O:10mL/L, trace element：10mL/L.Wherein micro- formula is Glycine：1.5g/L,MnSO4·
H2O:5.0g/L,CoCl2·6H2O:0.1g/L,CuSO4·5H2O:0.01g/L,KAl(SO4)2·12H2O:0.01g/L,
Na2MoO4·2H2O:0.01g/L,H3BO3:0.01g/L, pH=7.0, the saccharomyces cerevisiae spore content of picking is 2.0 × 106~
5.0×106Cfu/mL, towel gourd fritter dosage is 3~6 pieces/100mL nutrient solutions, at 25~30 DEG C, the fully lower culture of vibration
3~5d；
Preferably, 25~40mg/L of step 4) malachite green wastewater concentration, the culture maturation of the middle harvest of input step 3)
Towel gourd fritter, is added by the amount of 3~5 pieces/100mL waste water.
Compared with prior art, the invention has the advantages that：
(1) present invention is used for handling malachite green wastewater using the towel gourd that aging is discarded as fixation support, not only
It can solve the problems, such as that microorganism separation of solid and liquid is difficult, moreover it is possible to achieve the purpose that the treatment of wastes with processes of wastes against one another；
(2) the towel gourd load saccharomyces cerevisiae material that prepared by the present invention has efficient degradation effect to malachite green dyestuff；
(3) the towel gourd load saccharomyces cerevisiae material degradation malachite green dyestuff effect stability that prepared by the present invention, and can
Recycling is multiple, is a kind of economical and effective, environmentally friendly biomaterial；
(4) the towel gourd immobilized brewing yeast material preparation method that prepared by the present invention is simple, easy to operate.
Brief description of the drawings
Fig. 1 towel gourds load saccharomyces cerevisiae degrading malachite green waste water from dyestuff design sketch
Fig. 2 towel gourds load saccharomyces cerevisiae material recycling design sketch
Embodiment
The present invention is described in further detail below by drawings and examples：
Embodiment：
Malachite green dye waste water is derived from printing and dyeing mill's dyeing process waste water mouth, and it is 32mg/L to measure malachite green concentration.Tame and docile
Change S. cervisiae and prepare towel gourd load saccharomyces cerevisiae material, the material of preparation is given up for handling malachite green dyestuff
Water, using the experimental group for not adding material as control group, determines experimental group and control group malachite green degradation effect, experiment terminates
Afterwards, recycle towel gourd fritter and reuse the material process malachite green dye waste water.Comprise the following steps that：
(1) culture of saccharomyces cerevisiae spore：Solid medium culture saccharomyces cerevisiae spore is prepared, culture medium prescription is yeast
Powder 100mg/L, peptone 200mg/L, agar 180mg/L, malachite green 5mg/L~20mg/L (are stepped up), pH=7.0,
Condition of culture is 28 DEG C, incubation time 48h；
(2) selection and processing of towel gourd：Growth selection maturation can be used for the sponge gourd of breeding, and remove seed solarization dry doubling is cut into small
Block, specification are 3cm × 2cm × 1cm；
(3) preparation and its culture of towel gourd load saccharomyces cerevisiae material：Fluid nutrient medium is prepared, is formulated as glucose：
30g/L, NaNO3:3.0g/L,K2HPO4:1.0g/L,KCl:0.5g/L,MgSO4·7H2O:0.5g/L, 0.1%FeSO4·
7H2O:10mL/L, trace element：10mL/L.Wherein micro- formula is Glycine：1.5g/L,MnSO4·H2O:
5.0g/L,CoCl2·6H2O:0.1g/L,CuSO4·5H2O:0.01g/L,KAl(SO4)2·12H2O:0.01g/L,Na2MoO4·
2H2O:0.01g/L,H3BO3:0.01g/L, pH=7.0, add the 4 pieces/100mL of towel gourd fritter in (2), then in picking (1)
The saccharomyces cerevisiae spore of culture is added in culture medium so that saccharomyces cerevisiae spore amount is 2.5 × 106Cfu/mL, fully vibration,
3d is cultivated under conditions of 28 DEG C of temperature；
(4) towel gourd load saccharomyces cerevisiae material degradation malachite green dye waste water：Add into 100mL malachite green wastewaters
Enter the towel gourd load saccharomyces cerevisiae material (control group is not added with material) after 4 pieces of cultures, 28 DEG C, abundant oscillation treatment, exists respectively
0th, 1,2,4,8,12,15h samplings 3mL measures the absorbance of malachite green wastewater in 619nm at, calculates Each point in time and simulates
The concentration of waste water Malachite Green draws the degradation effect of malachite green, and experimental result is as shown in Figure 1, the results showed that the towel gourd
Fixed saccharomyces cerevisiae material can in 12h to the degradation effect of the malachite green dyestuff of 30mg/L up to 100%, and control group drops
Effect is solved less than 8%, and is no longer degraded substantially after 12h；
(5) after (4) Malachite Green dyestuff is degradable, towel gourd fritter is recycled, after washing 5 times with clear water, weight
Degrading malachite green waste water from dyestuff is used newly in, malachite green is equally measured at 619nm in 0,1,2,4,8,12,15h samplings 3mL
The absorbance of dyestuff, the concentration for calculating Each point in time waste water Malachite Green draw the degradation effect of malachite green dyestuff,
Experimental result is as shown in Figure 2, the results showed that the towel gourd fixes saccharomyces cerevisiae material in second for the first time of recycling, degraded
The effect of malachite green dyestuff does not decline, degradation effect of the third time recycling in 12h removal rates up to 83.25%, 15h
Up to 90%.I.e. towel gourd load saccharomyces cerevisiae material is steady with good degradation effect and material to malachite green dye waste water
It is qualitative good, it can reuse repeatedly.Therefore, improvement of the towel gourd load saccharomyces cerevisiae material in malachite green dye waste water
Aspect has huge potentiality.
Claims (6)
1. a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater, comprises the following steps：
The culture of step 1) saccharomyces cerevisiae spore：Prepare solid medium culture saccharomyces cerevisiae spore；
The selection and processing of step 2) towel gourd：Growth selection maturation can be used for the sponge gourd of breeding, and remove seed solarization dry doubling is cut into small
Block；
Step 3) towel gourd loads the preparation and its culture of saccharomyces cerevisiae material：Fluid nutrient medium is prepared, is added in step 2)
The saccharomyces cerevisiae spore of culture is added in culture medium in towel gourd fritter, then picking step 1), in 25~30 DEG C of temperature, liquid training
3~5d is cultivated under conditions of foster base pH=7.0；
Towel gourd load saccharomyces cerevisiae material is used for degrading malachite green waste water from dyestuff by step 4)：Clear water washing step 3) in receive
The towel gourd load saccharomyces cerevisiae material obtained 3~6 times, and thrown by the amount of 3~5 pieces of towel gourd fritter/100mL malachite green wastewaters
Towel gourd is added to load saccharomyces cerevisiae material, for degrading malachite green waste water from dyestuff；
Step 5) towel gourd loads the recycling of saccharomyces cerevisiae material：Step 4) Malachite Green waste water can qualified discharge
Afterwards, towel gourd fritter is recycled, is washed 3~6 times with clear water, the material of recycling is recycled and reused for malachite green dyestuff according to step 4)
The processing of waste water.
2. a kind of method for being used for degrading malachite green waste water using towel gourd load saccharomyces cerevisiae according to right 1, its
It is characterized in that：Step 1) solid culture based formulas is 80~120mg/L of dusty yeast, 180~220mg/L of peptone, agar 150~
200mg/L, malachite green 5~20mg/L of concentration, concentration step up, and condition of culture is 25~30 DEG C, and incubation time 48~
60h, it is spare under the conditions of being then stored in 4 DEG C.
3. a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater according to right 1, its
It is characterized in that：The selection and processing of step 2) towel gourd：Carrier of the sponge gourd of aging as saccharomyces cerevisiae is selected, by remove seed simultaneously
Towel gourd after drying is cut into fritter of uniform size, and size is 1cm × 1cm × 1cm~3cm × 2cm × 1cm.
4. a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater according to right 1, its
It is characterized in that：Step 3) towel gourd loads the preparation and its culture of saccharomyces cerevisiae material：The culture medium prescription of preparation is glucose：
30g/L,NaNO3:3.0g/L,K2HPO4:1.0g/L,KCl:0.5g/L,MgSO4·7H2O:0.5g/L, 0.1%FeSO4·
7H2O:10mL/L, trace element：10mL/L.Wherein micro- formula is Glycine：1.5g/L,MnSO4·H2O:
5.0g/L,CoCl2·6H2O:0.1g/L,CuSO4·5H2O:0.01g/L,KAl(SO4)2·12H2O:0.01g/L,Na2MoO4·
2H2O:0.01g/L,H3BO3:0.01g/L, pH=7.0, the saccharomyces cerevisiae spore content of picking is 2.0 × 106~5.0 ×
106Cfu/mL, towel gourd fritter dosage are 3~6 pieces/100mL nutrient solutions, and 3 are cultivated under 25~30 DEG C, abundant oscillating condition
~5d.
5. a kind of method for being used for degrading malachite green waste water using towel gourd fixation saccharomyces cerevisiae according to right 1, its
It is characterized in that：Step 4) malachite green wastewater concentration is 25~40mg/L, the ripe sponge gourd of the culture of harvest in input step 3)
Capsule fritter 3~5 pieces/100mL waste water.
6. a kind of method using towel gourd load saccharomyces cerevisiae material degradation malachite green wastewater according to right 1, its
It is characterized in that：After step 5) malachite green wastewater is degradable, strainer filters out towel gourd fritter, and towel gourd fritter is washed with clear water
3~6 times, recycling towel gourd fritter degrading malachite green waste water is carried out by step 4).
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5051481A (en) * | 1973-09-07 | 1975-05-08 | ||
| JP2003285088A (en) * | 2002-03-27 | 2003-10-07 | Kbe:Kk | Wastewater treatment method and treatment apparatus therefor |
| CN102059100A (en) * | 2010-12-10 | 2011-05-18 | 四川农业大学 | Method for preparing magnetic saccharomyces cerevisiae and technique for processing printing and dying wastewater by using same |
| CN103436481A (en) * | 2013-09-16 | 2013-12-11 | 江南大学 | Preparation method and application of brewer yeast spore serving as novel adsorbing agent |
| CN105483114A (en) * | 2016-01-20 | 2016-04-13 | 上海交通大学 | Microbial immobilization loofah sponge as well as preparation method and application thereof |
-
2017
- 2017-12-04 CN CN201711256381.XA patent/CN107986454B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5051481A (en) * | 1973-09-07 | 1975-05-08 | ||
| JP2003285088A (en) * | 2002-03-27 | 2003-10-07 | Kbe:Kk | Wastewater treatment method and treatment apparatus therefor |
| CN102059100A (en) * | 2010-12-10 | 2011-05-18 | 四川农业大学 | Method for preparing magnetic saccharomyces cerevisiae and technique for processing printing and dying wastewater by using same |
| CN103436481A (en) * | 2013-09-16 | 2013-12-11 | 江南大学 | Preparation method and application of brewer yeast spore serving as novel adsorbing agent |
| CN105483114A (en) * | 2016-01-20 | 2016-04-13 | 上海交通大学 | Microbial immobilization loofah sponge as well as preparation method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| E. K. MITTER,ETAL.: ""Acid Dye Biodegradation Using Saccharomyces cerevisiae Immobilized with Polyethyleneimine-Treated Sugarcane Bagasse "", 《WATER, AIR, & SOIL POLLUTION》 * |
| OGBONNA J C.ET AL.: "Loofa （luffa cylindrica） sponge as a carrier for microbial cell immobilization", 《JOURNAL OF FERMENTATION AND BIOENGINEERING》 * |
| 艾小林等: ""丝瓜络纤维的综合利用研究进展"", 《化工进展》 * |
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