CN108751424A - A method of utilizing heavy metal cadmium in wild soybean endogenetic fungus adsorbed water body - Google Patents
A method of utilizing heavy metal cadmium in wild soybean endogenetic fungus adsorbed water body Download PDFInfo
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- CN108751424A CN108751424A CN201810498402.7A CN201810498402A CN108751424A CN 108751424 A CN108751424 A CN 108751424A CN 201810498402 A CN201810498402 A CN 201810498402A CN 108751424 A CN108751424 A CN 108751424A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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Abstract
The invention discloses a kind of methods of heavy metal cadmium in endogenetic fungus adsorbed water body using wild soybean, select wild soybean the endophytic bacterial controlled effect Y2R14 and RWDL4-1 that early period, separation obtained, Sterile culture culture, it is placed in the PDB culture mediums containing different concentrations of cadmium chloride and cultivates, chlorination cadmium concentration 0mg/L~400mg/L gradients, 23~25 DEG C of cultivation temperature, 160r/min, 3d~15d is cultivated, measures the concentration of mycelia and the cadmium ion in bacterium solution respectively.When 200mg/L concentration of cadmium ions in solution, when cultivating 15d, concentration of cadmium ions significantly reduces in two strain cultured solutions, respectively 52mg/L and 68mg/L, is the 25~35% of original solution concentration of cadmium ions.Therefore, the invention can be used for the wastewater treatments such as heavy metal cadmium industry, agricultural, it can also be used to which sudden water body cadmium ion contamination accident has wide application and promotes space.
Description
Technical field
The invention belongs to soil, technical field of sewage, are related to a kind of utilization microorganism adsorption Heavy Metals in Waters cadmium
Pollution.
Background technology
A lot of heavy metal cadmium events have occurred in China various regions in recent years, and reducing heavy metal cadmium situation, oneself causes state
The great concern of family.The pollution of heavy metal cadmium includes mainly soil pollution and water pollution.Dust fall cadmium natural subsidence in air, aggregation
In the topsoil on factory periphery, the industrial wastewaters such as ore dressing, smelting, battery and plating are discharged to the surface water or the water that permeates the ground
It will produce soil pollution.In addition to this, long-term accumulation, the sprinkling etc. of phosphate fertilizer imposed with certain pesticides containing cadmium of cadmium waste residue,
It is an important factor for leading to Cadmium Pollution in Soils.Pollution of the cadmium to water body, mainly caused by industrial wastewater discharge, but mine is useless
The cadmium pollution especially severe of water, cadmium mobility are strong, are easy migration, seriously threaten plant growth, livestock and the life of the mankind.
The attention of people, biological adsorption are increasingly subject in waste water treatment currently with microorganism adsorption heavy metal ion
(Biosorption) it is a kind of emerging technology of processing effluent containing heavy metal ions pollution, it (is with microorganism by biological cell
It is main) or biomaterial adsorption heavy metal ion, it derives from a wealth of sources, it, can be by means of when the concentration of heavy metal ion in water is relatively low
Adsorption equilibrium concentrates or goes the heavy metal ion in water removal, can also further recycle heavy metal ion, it is considered to be one kind has very much
The technology of the replacement traditional treatment method of potentiality.In the prevalence of endophyte in plant, since it lives in no external sense
The health plant organization internal of symptom is contaminated, therefore the presence of endophyte of plant and effect are not taken seriously for a long time.It grinds in recent years
Study carefully the function of finding that endophyte of plant there are degradation environmental contaminants, the endophyte being such as separated to from Zhujiang River estuary mangrove has
The harmful substance in industrial wastewater is removed, purifying sea water is played the role of.And Barac etc. is research shows that certain endophytes can drop
Toluene is solved, and its host plant can be made to generate the resistance to toluene, this to carry out environment using microbial inoculant plant with regard to repairing
Possibility is provided again.In recent years, biological adsorption heavy metal is even more to have gradually formed an emerging research field, and start this
Method is used in the adsorption treatment of radionuclide ion, and the correlative study of wherein microbial adsorbent occupies significant proportion, so sharp
There is prodigious development potentiality with application of the resistant microorganism in heavy metal reparation field.
Invention content
1. technical problems to be solved by the inivention
It is difficult to adsorb and reduce the concentration of cadmium in water pollution for existing conventional chemistry techniques, the present invention provides a kind of utilization
The method that microorganism reduces heavy metal cadmium in solution realizes a purification huge sum of money by reducing the content of Heavy Metals in Waters cadmium
Belong to water pollution purpose.
2. technical solution
The principle of the present invention:Wild soybean endogenetic fungus Y2R14 and RWDL4-1, can grow under the conditions of high concentration cadmium,
And a large amount of cadmium ion in adsorbent solution, microbial cells are collected by filtration, reduce the cadmium ion in solution, the method is quick
Convenient, safe and efficient, environmental protection is at low cost.In order to achieve the above objectives, technical solution provided by the invention is as follows：
(1) PDB fluid nutrient mediums, 100ml triangular flasks are configured, every bottle of packing 50ml culture medium sterilizes spare.
(2) caddy mother liquor mother liquor, a concentration of 50mg/ml are prepared.
(3) it when bacterium colony when being covered on tablet, is beatened to take bacteria cake with card punch (diameter 0.8cm), every bottle of two bacteria cakes.Addition
Cadmium chloride solution adds mother liquor respectively according to 0mg/L, 50mg/L, 100mg/L, 200mg/L, 400mg/L gradient.Sealing packaging,
It is put on shaking table and cultivates, 160r/min cultivates 3d, 6d, 9d, 12d, 15d respectively.
(4) after the corresponding number of days of bacterial strain shake flask fermentation culture, strain growth situation is observed, obtains mycelia, bacterium solution respectively：With 4
Layer hospital gauze filters mycelia, distilled water flushing 3 times, and 60 DEG C of drying are weighed.
(5) concentration of cadmium ions in 3~15 days its mycelia of laggard one step surveying of bacterial strain shake flask fermentation culture and bacterium solution.
Description of the drawings
Fig. 1 is fermented and cultured different time mycelium morphology factor, D representative strains RWDL4-1；H representative strains Y2R14
Fig. 2 is cadmium ion changes of contents in fermented and cultured different time mycelia, D representative strains RWDL4-1；H representative strains
Y2R14
Fig. 3 is cadmium ion changes of contents in fermented and cultured different time culture solution, D representative strains RWDL4-1；H represents bacterium
Strain Y2R14
Specific implementation mode
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Specific implementation mode one：
1, wild soybean endogenetic fungal bacterial strain Y2R14 and RWDL4-1 is activated in PDA culture medium.25 DEG C, the normal feelings of illumination
It is cultivated under condition, it is spare after cultivating 7 days.
2, mother liquor is prepared：Caddy 50g/L solution.
PDB fluid nutrient mediums, 100ml triangular flasks are chosen, every bottle of packing 50ml culture medium sterilizes spare.Prepare caddy
Mother liquor (50g/L) accurately weighs caddy 1.50g and is dissolved in 30ml ddH2O, and sterilizing is spare.
The preparation of PDB culture mediums：Potato 200g, glucose 20g, distilled water 1000mL, natural pH.Weigh 200g Ma Ling
Potato, clean peeling are cut into small pieces, and add boiling rotten, with four layers of filtered through gauze, need to add glucose, continuation further according to actual experiment
Heating stirring is uniform, supplies moisture again to 1000mL after burning cooling, 250mL conical flasks is dispensed, wherein each conical flask dispenses
100mL PDB culture mediums.(121 DEG C) of wrapping sterilizing 20 minutes of jumping a queue is taken out, and is stored after slightly cooling down spare.
Specific implementation mode two：
Present embodiment is processing of the bacterial strain in cadmium chloride solution.Wait for activated strains bacterium colony when being covered on tablet,
It about 7 days or so, is beatened to take bacteria cake with card punch (diameter 0.8cm), every bottle of two bacteria cakes.Add cadmium chloride solution, according to 0mg/L,
50mg/L, 100mg/L, 200mg/L, 400mg/L gradient add mother liquor respectively, 25 DEG C, shaking speed 160r/min of cultivation temperature,
Culture 3d, 6d, 9d, 12d, 15d filterings harvest mycelia and bacterium solution respectively, are carried out using TAS-990 atomic absorption spectrophotometers
Concentration of cadmium ions measures (Beijing Pu Xi general finites company).
Analysis shows that 400mg/L cadmium chloride solutions can inhibit the growth of bacterial strain Y2R14 and RWDL4-1 mycelia, but two bacterial strains
It showing and extends with incubation time, inhibition level reduces, and two bacterial strains can still be grown in 400mg/L cadmium chloride solutions,
When cultivating 15 days, bacterial strain dry weight can reach 0.13~0.24g.Illustrate that the two bacterial strains have certain tolerance to cadmium ion.
In the fermented and cultured of the caddy containing 400mg/L, cadmium ion content is notable in bacterial strain Y2R14 and RWDL4-1 mycelia
Accumulation, Y2R14 bacterial strains cadmium ion content 14.47mg/g, 15.84mg/g, 18.573mg/g in fermented and cultured 6d, 9d and 15d,
It is 657 times, 160.6 times and 464.3 times of control respectively.Y2R14 bacterial strains cadmium ion content in fermented and cultured 6d, 9d and 15d is
12.08mg/g, 13.92mg/g, 9.405mg/g are 167.51 times, 327.75 times, 157.0 times of control respectively.
Cadmium ion content significantly drops in the fermented and cultured of caddy containing 200mg/L, bacterial strain Y2R14 and RWDL4-1 culture solution
It is low, RWDL4-1 bacterial strains cadmium ion content 200mg/L, 137.04mg/L, 120.67mg/ in fermented and cultured 0d, 6d, 9d and 15d
L,68mg/L.Y2R14 bacterial strains cadmium ion in fermented and cultured 0d, 6d, 9d and 15d content be 200mg/L, 92.26mg/L,
67.6mg/L 52.0mg/L.The result illustrates that two bacterial strains have very strong enrichment and absorption cadmium ion ability, Ke Yi again
It is used alone or is used in combination in the dirty water decontamination handles.
In conclusion wild soybean plant of two plants endogenetic fungus used in the present invention from spontaneous growth is strong
Health tissue, non-cause of disease class fungi can play protection and defense reaction to plant, to person poultry harmless, and show strong cadmium ion
Tolerance and enrichment can quickly reduce concentration of cadmium ions in solution.It therefore, can using this two plants of wild soybean endogenetic fungus
Achieve the purpose that reduce Heavy Metals in Waters cadmium pollution, method handy and safe, economical and efficient.
The above is only the specific implementation mode of the present invention, it is noted that due to the finiteness of literal expression, for this
For the those of ordinary skill of technical field, without departing from the principles of the present invention, several improvement and optimization can also be made,
Above-mentioned technical characteristic can also be combined in the right way；Or it is not improved that the design of invention and technical solution is direct
Applied to other occasions, it is accordingly to be regarded as protection scope of the present invention.
Claims (1)
1. a kind of method of heavy metal cadmium in endogenetic fungus adsorbed water body using wild soybean, it is characterised in that：Pass through profit
With cadmium ion in wild soybean endogenetic fungus adsorbent solution, the concentration of cadmium ion is reduced, achievees the purpose that purify sewage；
A kind of method using microorganism adsorption heavy metal cadmium according to claim 1, it is characterized in that：
From laboratory wild soybean endophyte bacterial strain Y2R14 and RWDL4-1 that early period, separation obtained, wait for bacterium colony on tablet
It when covering with, is beatened to take bacteria cake, is placed in the PDB culture mediums containing different concentrations of cadmium chloride and cultivates, chlorination cadmium concentration 0mg/L~400mg/
L gradients, 25 degree, 160r/min of cultivation temperature cultivate 3d~15d, observe strain growth situation, obtain mycelia and bacterium solution respectively,
Measure the concentration of the cadmium ion in bacterium solution；The strong tolerance cadmium-ion solution of bacterial strain Y2R14 and RWDL4-1 performance, is cultivated 6 days~15 days
Growth faster, and cadmium ion in energy significant enrichment and adsorption aqueous solution can be carried out in the process；When cadmium containing 200mg/L in solution
When ion concentration, when cultivating 15 days, concentration of cadmium ions significantly reduces in two strain cultured solutions, respectively 52mg/L and
68mg/L is the 25~35% of original solution concentration of cadmium ions.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810498402.7A CN108751424A (en) | 2018-05-23 | 2018-05-23 | A method of utilizing heavy metal cadmium in wild soybean endogenetic fungus adsorbed water body |
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| CN201810498402.7A CN108751424A (en) | 2018-05-23 | 2018-05-23 | A method of utilizing heavy metal cadmium in wild soybean endogenetic fungus adsorbed water body |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706211A (en) * | 2019-01-07 | 2019-05-03 | 湖南泰谷生态工程有限公司 | Shake flask re-screening method for cadmium activated microorganisms |
| CN113263049A (en) * | 2021-07-13 | 2021-08-17 | 河南农业大学 | Application of calcium chloride in enhancing cadmium reduction capability of fungal soil and method |
-
2018
- 2018-05-23 CN CN201810498402.7A patent/CN108751424A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109706211A (en) * | 2019-01-07 | 2019-05-03 | 湖南泰谷生态工程有限公司 | Shake flask re-screening method for cadmium activated microorganisms |
| CN109706211B (en) * | 2019-01-07 | 2022-05-13 | 湖南重金属污染耕地安全高效利用工程研究中心有限公司 | Shake flask re-screening method for cadmium activated microorganisms |
| CN113263049A (en) * | 2021-07-13 | 2021-08-17 | 河南农业大学 | Application of calcium chloride in enhancing cadmium reduction capability of fungal soil and method |
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