CN103667112B - A kind of for the composite flora of electroplating wastewater and preparation method thereof of degrading - Google Patents
A kind of for the composite flora of electroplating wastewater and preparation method thereof of degrading Download PDFInfo
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Abstract
The invention discloses a kind of for the composite flora of electroplating wastewater and preparation method thereof of degrading, do you comprise the steps: picking agrobacterium (Agrobacterium respectively? sp.), rod bacterium (Bacillus? sp.), pseudomonas putida (Pseudomonas? putida.), Pseudomonas stutzeri (Pseudomonas? stutzeri.) inoculated and cultured; By volume percentage meter, do you get respectively 15～17% agrobacterium (Agrobacterium? sp.), 3～17% rod bacterium (Bacillus? sp.), 23～32% pseudomonas putida (Pseudomonas? putida) and 34～59% Pseudomonas stutzeri (Pseudomonas? stutzeri.), after activation, mix and put in waste water. The present invention, by the coordinative role between bacterial classification, can effectively improve the degradation efficiency of microbiological treatment electroplating wastewater.
Description
Technical field
The present invention relates to electroplating wastewater processing field, specifically relate to a kind of composite flora for the electroplating wastewater of degrading andIts preparation method.
Background technology
Due to not biodegradable, bioconcentration of heavy metal ion, as the key industry of heavy metal pollution, electroplateOne of contaminated industry that the Yi Shi world today is the most serious, the governing problem of the heavy metal wastewater thereby producing has become field of Environment Protection and has closedThe focus of note. The method of processing traditionally heavy metal wastewater thereby is mainly physical-chemical process, as absorption method, ion-exchange, chemistryThe precipitation method, membrane separation process, oxidation-reduction method etc. But the problems such as it is serious that these methods all have secondary pollution, processing cost height.
The biological treatment process of electroplating wastewater is mainly by organism, absorption naturally, the organism metabolism of metal to be producedThe precipitation of thing to metal, the albumen in organism and the combination of metal, and heavy metal is in vivo under enzyme effectTransform the effect of grade the heavy metal pollutants in waste water is decomposed, absorbed, administer thereby reach the object of polluting. Biological treatmentCompared with additive method, its cost is low, and efficiency is high, and easily operation, and the most important thing is does not have secondary pollution, therefore, and uselessIn water treatment, be widely used.
Along with expanding economy, the composition of waste water is day by day complicated, especially when containing organic dirt poisonous, difficult degradation in waste waterWhile dying thing, due to kind, the negligible amounts of the microorganism this type organic to special degradation capability in environment, simultaneously itIn interspecies competition, be in a disadvantageous position, therefore, traditional biologic treating technique faces big challenge.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide a kind of degradation efficiency high, expense low for fallingComposite flora of electrolytic plating waste water and preparation method thereof.
Object of the present invention is achieved through the following technical solutions:
For a preparation method for the composite flora of the electroplating wastewater of degrading, comprise the steps:
The preparation of (1) four kind of bacterium exponential phase cell:
Picking agrobacterium (Agrobacteriumsp.) respectively, rod bacterium (Bacillussp.), pseudomonas putida(Pseudomonasputida.), four kinds of bacterium of Pseudomonas stutzeri (Pseudomonasstutzeri.), 1～2 ring, by its pointDo not transfer to containing in 20～30mL nutrient solution, every kind of bacterium is cultivated 1～2 day under the condition of 27～35 DEG C; Again by cultivate afterFour kinds of bacterium are seeded to containing 300～500mL propagation and cultivate with the ratio of 1:9～1:12 respectively in thalline and proliferated culture medium volume ratioIn the container of base, adopt different proliferated culture mediums to cultivate, wherein, the nutrient medium of agrobacterium and rod bacterium is beef extract3.0～4.0g/L, tryptone 4.0～5.0g/L, all the other are water; Pseudomonas putida nutrient medium be beef extract 1.5g～2.0/L, glucose 1.0～2.0g/L, tryptone 6.0～7.0g/L, dusty yeast 3.0～4.0g/L, all the other are water; Amur vacationMonad nutrient medium is fresh potato juice 800～1000mL, glucose 16～20g, and all the other are water; Then 27～35DEG C condition under cultivate 1～2 day, after the centrifugal 10～15min of speed with 6000～7000rpm, obtain respectively above-mentioned four kinds of bacteriumThe exponential phase cell of body; With densimeter, the main component of described nutrient solution is beef extract 1.5～2.0g/L, glucose 1.0～2.0g/L, tryptone 5.5～6.5g/L, dusty yeast 3.0～4.0g/L; PH6.5～7.5, all the other are water;
The mixed bacterial of (2) four kinds of thalline composite: the exponential phase cell of four kinds of thalline is taken out, slow with phosphateRush liquid washing 2～3 times, then percentage meter by volume, gets respectively 9～17% agrobacteriums (Agrobacteriumsp.), 3～17% rod bacterium (Bacillussp.), 23～32% pseudomonas putidas (Pseudomonasputida) and 34～59% AmursPseudomonad (Pseudomonasstutzeri.) mixes, the composite flora of the electroplating wastewater that must be used for degrading.
Further, the degraded of described composite flora is during containing electroplating wastewater, and the mixed bacteria liquid of getting composite flora is with 1:15～1:30 volume ratio is added in nutrient solution and cultivates 6～12h, makes directly to put into electroplating wastewater after composite flora activationIn, every 1 liter of electroplating wastewater adds mixed bacteria liquid 1～2mL; Air Exposure 24～48h, aeration rate is 2～4L/h. Described nutrient solutionMain component is beef extract 1.5～2.0g/L, glucose 1.0～2.0g/L, and tryptone 5.5～6.5g/L, dusty yeast 3.0～4.0g/L, pH6.5～7.5, all the other are water.
By volume percentage meter, the composition of described phosphate buffer is sodium chloride 8.0～9.0g/L, potassium chloride 0.2～0.3g/L, dipotassium hydrogen phosphate 1.1～1.2g/L and potassium dihydrogen phosphate 0.2～0.3g/L, all the other are water. Described phosphate bufferWashing times be 2～3 times.
Described fresh potato juice is prepared by the following method: removes 160～200 grams of skin fresh potatos, is cut into small pieces,Add deionized water 800～1000mL and boil 20 ?40 minutes, elimination potato ball, with deionized water filtrate is complemented to 800～1000mL。
For a composite flora for the electroplating wastewater of degrading, made by above-mentioned preparation method.
The present invention adds microorganism or some matrix with specific function in traditional biological treatment system, strengthens itTo the degradation capability of specific pollutants, thereby improve the treatment effect of whole sewage disposal system, this technology is called biological strongChange technology.
Tool of the present invention has the following advantages:
1) agrobacterium (Agrobacteriumsp.) that utilizes provided by the invention, rod bacterium (Bacillussp.), dislikesSmelly pseudomonad (Pseudomonasputida.), four kinds of bacterium of Pseudomonas stutzeri (Pseudomonasstutzeri.) are by oneCertainty ratio composition advantage composite flora adds the biological reinforced processing of carrying out waste water: pseudomonas putida is often used in waste water placeReason, it has the ability of degraded for example toluene of arene and phenol, and these are not all the common pollutants in municipal wastewater, in addition notFew researcher finds that by research pseudomonas putida has very strong tolerance to tens of metal ion species such as lead, cadmium, copper, and rightThese heavy metals have certain degradation; Rod bacterium is a kind of common soil bacteria and can makes aromatic hydrocarbon generation secondary fallSeparate; Agrobacterium has good suction-operated to various heavy such as copper, chromium, cadmium and nickel; Pseudomonas stutzeri is in waterPhenanthrene, pentachlorophenol and sulfurous organic compound have good degradation effect. The present invention finds that the degradation effect of composite flora is far above appointingWhat single bacterial strain of a strain, illustrates and between different microorganisms, produces certain interaction. This is because many biological activities are singleStrain Institute of Micro-biology can not complete or can only faintly carry out, must be by two or more microorganisms in same environment mutuallyBe used for realizing. Adopt single flora and genetic engineering bacterium to carry out in contaminant degradation process, usually due to generation in the middle of inhibitionGeneration or the intermediate product of thanking to product enter cut-off type metabolite approach (endproductpathways) and have suppressed dirtDye thing degrading enzymatic activity, make contaminant degradation efficiency not high or degraded not thorough. Utilize interaction useful between microorganism,By microorganism Mixed culture and domestication, bacterium is flowed to and carries out some improvement the mesostate of pollutant, make inhibitionIntermediate product does not generate or transforms as early as possible, thereby improves contaminant degradation efficiency. Therefore, utilize microbial interaction, micro-The mixed cell microorganism adopting in biological practical application is cultivated method for domesticating and has more significance.
2) the present invention finds these a few strain bacterium they has certain degradation capability to the different component of electroplating wastewater separately, asPseudomonas putida and Pseudomonas stutzeri can produce wooden equal hexichol for Yi Xi ?α ， β ?the peroxidase such as dioxygenase. ThisA little enzymes can attack simultaneously ehter bond in lignin structure, C ?C key and Cα‐CβTwo keys, are destroyed rapidly lignin structure, and thenBecome small organic molecule. Agrobacterium and rod bacterium can utilize the little molecule that in waste water, larger molecular organics degraded generatesOrganic matter carries out growth metabolism as carbon source, and then is further degraded into the inorganic matter such as carbon dioxide and water. Simultaneously a large amount ofAgrobacterium and the rod bacterium of breeding can secrete a large amount of polysaccharose substance with flocculation and protein substance. TheseSecretion can precipitate with adsorbed water in heavy metal ion, add the counterweight metal of microorganism own and there is certain absorption and doWith, thereby reach the object of removing heavy metal in waste water. Between their growth substrate, there is no competitiveness, so can well assistWith growth. Due to the different organic matter of variant strains for degrading, their synergy just have the pollutant of electroplating wastewaterVery high degradation rate.
Brief description of the drawings
Fig. 1 is four kinds of growing state figure that thalline is cultivated in aseptic proliferated culture medium in embodiment 1.
Fig. 2 is four kinds of growing state figure that thalline is cultivated in aseptic proliferated culture medium in embodiment 2.
Fig. 3 is four kinds of growing state figure that thalline is cultivated in aseptic proliferated culture medium in embodiment 3.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further illustrated, but the scope of protection of present invention is notBe confined to the scope of embodiment statement.
In the present embodiment, electroplating wastewater is that Treatment of Metal Surface factory handware plating production process produces because plating piece cleansWaste water, add before mixed bacteria liquid in Electroplate Factory through processing such as contact break, broken cyanide and coagulating sedimentation. This waste water main component isCu2+Concentration is 5～8mg/L, Ni2+Concentration 13～18mg/L, total chromium concn 15～22mg/L, Cd2+Concentration 6～8mg/L,COD156～243mg/L, pH is 6～8.
Embodiment 1:
Picking agrobacterium (Agrobacteriumsp.) respectively, rod bacterium (Bacillussp.), pseudomonas putida(Pseudomonasputida.), Pseudomonas stutzeri (Pseudomonasstutzeri.) is (from electroplating the discharge outlet of factoryMud in separate obtain) four kinds of bacterium 1 encircle, by its transfer to respectively containing 20mL nutrient solution (its composition is 1.5g/L beef extract,1.0g/L glucose, 5.5g/L tryptone, 3.0g/L dusty yeast, pH6.5, all the other are water) container in, every kind of bacterium existsUnder the condition of 27 DEG C, cultivate 1 day, then four kinds of bacterium after cultivating are connect with the volume ratio (thalline and proliferated culture medium) of 1:9 respectivelyPlant bacteria suspension to containing in the container of the aseptic proliferated culture medium of 500mL, under the condition of 35 DEG C, cultivate 1 day, with the speed of 6000rpmAfter centrifugal 10min, obtain respectively the exponential phase cell of above-mentioned four kinds of thalline. Adopt different propagation nutrient medium trainingsEducate, wherein, the nutrient medium of agrobacterium and rod bacterium is beef extract 3.0g/L, tryptone 4.0g/L, and all the other are water; DislikeSmelly pseudomonad nutrient medium is beef extract 1.5g/L, glucose 1.0g/L, and tryptone 6.0g/L, dusty yeast 3.0g/L,All the other are water; Pseudomonas stutzeri and pseudomonas putida nutrient medium are fresh potato juice 800mL, glucose 16g, itsYu Weishui. Suitably cultivate the growing state of rear four kinds of bacteriums as shown in Figure 1. As can be seen from Figure 1, the growth song of four kinds of bacteriumsLine all obviously presents lag phase, logarithmic phase and stationary phase, but different strain reaches required Time Inconsistency in each period.Agrobacterium (Agrobacteriumsp.) enters exponential phase at cultivation 4h, enters stationary phase, rod bacterium after 20h(Bacillussp.) enter exponential phase at cultivation 2h, after 18h, enter stationary phase, pseudomonas putida (PseudomonasPutida) after cultivation 5h, just enter exponential phase, 12h enters stationary phase, Pseudomonas stutzeri (PseudomonasStutzeri.) after cultivation 2h, just enter exponential phase, 8h enters stationary phase.
The exponential phase cell of above-mentioned four kinds of thalline is taken out, with phosphate buffer (its main component sodium chloride8.0g/L, potassium chloride 0.2g/L, dipotassium hydrogen phosphate 1.1g/L and potassium dihydrogen phosphate 0.2g/L, all the other are water) wash after 2 times, pressPercent by volume meter, gets respectively 15% agrobacterium (Agrobacteriumsp.), 3% rod bacterium (Bacillussp.), 23%Pseudomonas putida (Pseudomonasputida) and 59% Pseudomonas stutzeri (Pseudomonasstutzeri.) mix,And be suspended in physiological saline, refrigerate for subsequent use;
The volume ratio (mixed bacteria liquid and nutrient solution) that mixed bacteria liquid obtained above is got with 1:20 adds to nutrient solutionMiddle cultivation makes, after its activation, directly to put in electroplating wastewater for 6 hours, carries out Air Exposure 24h, and aeration rate is 4L/h. With matterAmount densimeter, the composition of nutrient solution is 1.5g/L beef extract, 1.0g/L glucose, 5.5g/L tryptone, 3.0g/L dusty yeast,PH6.5, all the other are water.
Its water quality of electroplating wastewater 1000ml(that adopts the present embodiment method to process plating piece cleaning generation is Cu2+Concentration is5mg/L，Ni2+Concentration 18mg/L, total chromium concn 15mg/L, Cd2+Concentration 6mg/L, COD156mg/L, pH is 6), and not haveAdd the experiment of composite flora as a control group, simultaneously with add respectively single culture and contrast. All experiment aeration rates are equalFor 4L/h, aeration after 24 hours result as shown in table 1. Wherein, the mensuration of COD adopt potassium dichromate method (GB/T16157 ?1996), the mensuration of nickel adopts atomic absorption spectrophotometry (GB/T11912 ?1989), and the mensuration of copper and cadmium adopts formerSub-absorptiometry (GB/T7475 ?1987), the mensuration of total chromium adopt Gao potassium manganate Yangization ?diphenylcarbazide light splittingPhotometry (GB/T7566 ?1987).
Table 1 is tested comparing result
The result of table 1 shows that composite flora is better than contrast far away and adds separately single bacterium the water treatment effect of electroplatingKind. Existence between compound bacteria masses' bacterial classification interacts, and the interaction energy between bacterial classification impels bacterium to produce outside more born of the same parentsThe protein that polymer has been correlated with, these protein and cell extracellular polymeric have stronger Adsorption and flocculation effect, haveHelp the removal to COD in electroplating wastewater and heavy metal ion.
Embodiment 2
Four kinds of bacterium 2 of picking encircle respectively: agrobacterium (Agrobacteriumsp.), rod bacterium (Bacillussp.), dislikesSmelly pseudomonad (Pseudomonasputida.), Pseudomonas stutzeri (Pseudomonasstutzeri.) is (from galvanizerThe discharge outlet of factory
Mud in separate obtain), by its transfer to respectively containing 30mL nutrient solution (its composition is 2.0g/L beef extract,1.5g/L glucose, 6.5g/L tryptone, 3.5g/L dusty yeast, pH7.5, all the other are water and requisite impurity)In container, every kind of bacterium is cultivated 2 days under the condition of 30 DEG C, then by four kinds of bacterium after cultivating respectively with the volume ratio of 1:10(thalline and proliferated culture medium) inoculation bacteria suspension is to cultivating under the condition of 35 DEG C containing in the container of aseptic 500mL proliferated culture medium2 days, after the centrifugal 15min of speed with 7000rpm, obtain respectively the exponential phase cell of above-mentioned four kinds of thalline. Wherein four kindsDifferent bacteriums adopts different proliferated culture mediums, and wherein the nutrient medium of agrobacterium and rod bacterium is beef extract 3.5g/L, tryptone 5.0g/L, all the other are water; Pseudomonas putida nutrient medium is beef extract 2.0/L, glucose 1.5g/L, pancreasPeptone 6.5g/L, dusty yeast 4.0g/L, all the other are water; Pseudomonas stutzeri nutrient medium is fresh potato juice 900mL,Glucose 20g, all the other are water. Suitably cultivate the growing state of rear four kinds of bacteriums as shown in Figure 2. As can be seen from Figure 2, four kindsThe growth curve of bacterium all obviously presents lag phase, logarithmic phase and stationary phase, required but different strain reaches each periodTime Inconsistency. Agrobacterium (Agrobacteriumsp.) enters exponential phase at cultivation 4h, enters stable after 15hPhase, rod bacterium (Bacillussp.) enters exponential phase at cultivation 2h, enters stationary phase, pseudomonas putida after 18h(Pseudomonasputida) after cultivation 5h, just enter exponential phase, 16h enters stationary phase, Pseudomonas stutzeri(Pseudomonasstutzeri.) after cultivation 2h, just enter exponential phase, 10h enters stationary phase.
The exponential phase cell of above-mentioned four kinds of thalline is taken out, with phosphate buffer (its main component sodium chloride9.0g/L, potassium chloride 0.25g/L, dipotassium hydrogen phosphate 1.2g/L and potassium dihydrogen phosphate 0.25g/L, all the other are water) wash after 3 times,By volume percentage meter, gets respectively 16% agrobacterium (Agrobacteriumsp.), 12% rod bacterium (Bacillussp.),27% pseudomonas putida (Pseudomonasputida) and 45% Pseudomonas stutzeri (Pseudomonasstutzeri.) are mixedClose, and be suspended in physiological saline, refrigerate for subsequent use.
The volume ratio (mixed bacteria liquid and nutrient solution) that mixed bacteria liquid obtained above is got with 1:15 adds to nutrient solutionMiddle cultivation makes, after its activation, directly to put in electroplating wastewater for 12 hours, carries out aeration 48h, and aeration rate is 2L/h. Dense with qualityDegree meter, the composition of nutrient solution is 2.0g/L beef extract, 1.5g/L glucose, 6.5g/L tryptone, 3.5g/L dusty yeast, pH7.5, all the other are water.
Adopt the present embodiment method to process plating piece and clean the electroplating wastewater 1000ml producing, (Cu in water quality2+Concentration is 8mg/L，Ni2+Concentration 15mg/L, total chromium concn 22mg/L, Cd2+Concentration 7mg/L, COD208mg/L, the electroplating wastewater that pH is 71000ml, and with the experiment that do not add composite flora as a control group. Two groups of experiment aeration rates are 2L/h, after aeration 24hResult is as shown in table 2. Wherein, the mensuration of COD adopts potassium dichromate method (GB/T16157 ?1996), and the mensuration of nickel adopts flameAtomic absorption spectrophotometry (GB/T11912 ?1989), the mensuration of copper and cadmium adopts atomic absorption spectrophotometry (GB/T7475 ?1987), the mensuration of total chromium adopt Gao potassium manganate Yangization ?diphenyl carbazide spectrophotometry (GB/T7566 ?1987）。
Table 2 is tested comparing result
The result of table 2 shows that composite flora is better than contrast far away and adds separately single bacterium the water treatment effect of electroplatingKind. Existence between compound bacteria masses' bacterial classification interacts, and the interaction energy between bacterial classification impels bacterium to produce outside more born of the same parentsThe protein that polymer has been correlated with, these protein and cell extracellular polymeric have stronger Adsorption and flocculation effect, haveHelp the removal to COD in electroplating wastewater and heavy metal ion.
Embodiment 3
Four kinds of bacterium 1 of picking encircle respectively: agrobacterium (Agrobacteriumsp.), rod bacterium (Bacillussp.), dislikesSmelly pseudomonad (Pseudomonasputida.), Pseudomonas stutzeri (Pseudomonasstutzeri.) is (from galvanizerIn the mud of the discharge outlet of factory, separate and obtain), it is transferred to respectively to (its composition is 1.7g/L beef containing 25mL nutrient solutionCream, 2.0g/L glucose, 6.0g/L tryptone, 4.0g/L dusty yeast, pH7.0, all the other are water) container in, every kind of bacteriumUnder the condition of 27 DEG C, cultivate 2 days, then with the volume ratio of 1:12, (thalline is cultivated with propagation respectively by four kinds of bacterium after cultivatingBase) inoculate bacteria suspension to cultivating 2 days containing in the container of aseptic 450mL proliferated culture medium, with 6000rpm's under the condition of 30 DEG CAfter the centrifugal 15min of speed, obtain respectively the exponential phase cell of above-mentioned four kinds of thalline. Wherein four kinds of different bacteriums adopt notSame proliferated culture medium, wherein the nutrient medium of agrobacterium and rod bacterium is beef extract 4.0g/L, tryptone 4.5g/L,All the other are water; Pseudomonas putida nutrient medium is beef extract 1.8g/L, glucose 2.0g/L, tryptone 7.0g/L, fermentFemale powder 3.5g/L, all the other are water; The nutrient medium fresh potato juice 1000mL of Pseudomonas stutzeri, glucose 18g, all the otherFor water. Suitably cultivate the growing state of rear four kinds of bacteriums as shown in Figure 3. As can be seen from Figure 3, the growth curve of four kinds of bacteriumsAll obviously present lag phase, logarithmic phase and stationary phase, but different strain reaches required Time Inconsistency in each period. SoilEarth bacillus (Agrobacteriumsp.) enters exponential phase at cultivation 2h, enters stationary phase, rod bacterium after 12h(Bacillussp.) enter exponential phase at cultivation 2h, after 18h, enter stationary phase, pseudomonas putida (PseudomonasPutida) after cultivation 3h, just enter exponential phase, 12h enters stationary phase, Pseudomonas stutzeri (PseudomonasStutzeri.) after cultivation 5h, just enter exponential phase, 12h enters stationary phase.
The exponential phase cell of above-mentioned four kinds of thalline is taken out, with phosphate buffer (its main component sodium chloride8.5g/L, potassium chloride 0.3g/L, dipotassium hydrogen phosphate 1.15g/L and potassium dihydrogen phosphate 0.3g/L, all the other are water) wash after 3 times, pressPercent by volume meter, gets respectively 9% agrobacterium (Agrobacteriumsp.), 13% rod bacterium (Bacillussp.), 30%Pseudomonas putida (Pseudomonasputida) and 48% Pseudomonas stutzeri (Pseudomonasstutzeri.) mix,And be suspended in physiological saline, refrigerate for subsequent use;
The volume ratio (mixed bacteria liquid and nutrient solution) that mixed bacteria liquid obtained above is got with 1:25 adds to nutrient solutionMiddle cultivation makes, after its activation, directly to put in electroplating wastewater for 8 hours, carries out aeration 36h, and aeration rate is 3L/h. Dense with qualityDegree meter, the composition of nutrient solution is 1.7g/L beef extract, 2.0g/L glucose, 6.0g/L tryptone, 4.0g/L dusty yeast, pH7.0, all the other are water.
Adopt the present embodiment method to process plating piece and clean the electroplating wastewater 1000ml producing, (its water quality is Cu2+Concentration is8mg/L，Ni2+Concentration 13mg/L, total chromium concn 17mg/L, Cd2+Concentration 8mg/L, COD243mg/L, pH is 8), and not haveAdd the experiment of composite flora as a control group. Two groups experiment aeration rates be 3L/h, aeration after 36 hours result as table 3Show. Wherein, the mensuration of COD adopts potassium dichromate method (GB/T16157 ?1996), and the mensuration of nickel adopts Flame Atomic Absorption Spectrometry light splittingPhotometry (GB/T11912 ?1989), the mensuration of copper and cadmium adopts atomic absorption spectrophotometry (GB/T7475 ?1987), totalThe mensuration of chromium adopt Gao potassium manganate Yangization ?diphenyl carbazide spectrophotometry (GB/T7566 ?1987).
3 table experiment comparing results
The result of table 3 shows that composite flora is better than contrast far away and adds separately single bacterium the water treatment effect of electroplatingKind. Existence between compound bacteria masses' bacterial classification interacts, and the interaction energy between bacterial classification impels bacterium to produce outside more born of the same parentsThe protein that polymer has been correlated with, these protein and cell extracellular polymeric have stronger Adsorption and flocculation effect, haveHelp the removal to COD in electroplating wastewater and heavy metal ion. Above-described embodiment is preferably embodiment of the present invention, but thisBright embodiment is not restricted to the described embodiments, and other any do not deviate from institute under Spirit Essence of the present invention and principleThe change, modification done, substitute, combination, simplify, all should be equivalent substitute mode, be included in protection scope of the present invention itIn.
In the present invention, the microbiologic population forming by optimum organization can realize the collaborative of four kinds of microorganisms in systemWith complementation, overcome single culture to organic matter degradation in electroplating wastewater and the inefficient problem of heavy metal, make whole systemSystem all has efficient degradation capability to electroplating wastewater. Wherein CODCrClearance is 75～85%, Cu2+Except rate is 78～83%, Ni2 +Clearance is 79～89%, and total chromium clearance is 77～82%, Cd2+Clearance is 75～81%. And single bacterium is to electroplating wastewaterTreatment effect be starkly lower than composite flora of the present invention, therefore this composite flora process have aspect electroplating wastewater goodApplication prospect.
Claims (5)
1. for a preparation method for the composite flora of the electroplating wastewater of degrading, it is characterized in that comprising the steps:
The preparation of (1) four kind of bacterium exponential phase cell:
Picking agrobacterium (Agrobacteriumsp.), rod bacterium (Bacillussp.), pseudomonas putida respectively(Pseudomonasputida.) and four kinds of bacterium of Pseudomonas stutzeri (Pseudomonasstutzeri.) 1～2 ring, by its pointDo not transfer to containing in 20～30mL nutrient solution, every kind of bacterium is cultivated 1～2 day under the condition of 27～35 DEG C; Again by cultivate afterFour kinds of bacterium are seeded to containing 300～500mL nutrition and cultivate with the ratio of 1:9～1:12 respectively in thalline and nutrient medium volume ratioIn the container of base, adopt different nutrient mediums to cultivate, wherein, the nutrient medium of agrobacterium and rod bacterium is beef extract3.0～4.0g/L, tryptone 4.0～5.0g/L, all the other are water; Pseudomonas putida nutrient medium be beef extract 1.5g～2.0/L, glucose 1.0～2.0g/L, tryptone 6.0～7.0g/L, dusty yeast 3.0～4.0g/L, all the other are water; Amur vacationMonad nutrient medium is fresh potato juice 800～1000mL, glucose 16～20g, and all the other are water; Then 27～35DEG C condition under cultivate 1～2 day, after the centrifugal 10～15min of speed with 6000～7000rpm, obtain respectively above-mentioned four kinds of bacteriumThe exponential phase cell of body; With densimeter, the main component of described nutrient solution is beef extract 1.5～2.0g/L, glucose 1.0～2.0g/L, tryptone 5.5～6.5g/L, dusty yeast 3.0～4.0g/L; PH6.5～7.5, all the other are water;
The mixed bacterial of (2) four kinds of thalline composite: the exponential phase cell of four kinds of thalline is taken out, use phosphate bufferWash 2～3 times, then percentage meter by volume, gets respectively 9～17% agrobacteriums (Agrobacteriumsp.), 3～17% rod bacterium (Bacillussp.), 23～32% pseudomonas putidas (Pseudomonasputida) and 34～59% are executedFamily name pseudomonad (Pseudomonasstutzeri.) mixes, the composite flora of the electroplating wastewater that must be used for degrading.
2. the preparation method of the composite flora for the electroplating wastewater of degrading according to claim 1, is characterized in that, described inWhen composite flora degraded contains electroplating wastewater, the mixed bacteria liquid of getting composite flora is added to nutrition with the volume ratio of 1:15～1:30In liquid, cultivate 6～12h, make directly to put in electroplating wastewater after composite flora activation, every 1 liter of electroplating wastewater adds mixingBacterium liquid 1～2mL; Air Exposure 24～48h, aeration rate is 2～4L/h.
3. the preparation method of the composite flora for the electroplating wastewater of degrading according to claim 1, is characterized in that, by bodyLong-pending percentage meter, the composition of described phosphate buffer is sodium chloride 8.0～9.0g/L, potassium chloride 0.2～0.3g/L, phosphoric acid hydrogenDipotassium 1.1～1.2g/L and potassium dihydrogen phosphate 0.2～0.3g/L, all the other are water.
4. the preparation method of the composite flora for the electroplating wastewater of degrading according to claim 1, is characterized in that, described inFresh potato juice is prepared by the following method: remove 160～200 grams of skin fresh potatos, be cut into small pieces, add deionized water800～1000mL, boils 20 ?40 minutes, and elimination potato ball complements to 800～1000mL by filtrate by deionized water.
5. for a composite flora for the electroplating wastewater of degrading, it is characterized in that, by preparation method described in claim 1,3 or 4Make.
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| CN107189965A (en) * | 2017-07-12 | 2017-09-22 | 国网山东省电力公司临沂供电公司 | A kind of biological agent for being used to repair power plant's sanitary wastewater |
| CN109516613A (en) * | 2018-11-23 | 2019-03-26 | 宁波欣辉环保科技有限公司 | A kind of cyanide containing wastewater plating pretreating process |
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| CN102390889A (en) * | 2011-09-26 | 2012-03-28 | 华南理工大学 | Method for treating pulping and papermaking wastewater by using dominant degradation bacteria |
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