CN101054242B - Application of planococcus psychrotoleratus in treating sewage at low temperature - Google Patents
Application of planococcus psychrotoleratus in treating sewage at low temperature Download PDFInfo
- Publication number
- CN101054242B CN101054242B CN200710065134.1A CN200710065134A CN101054242B CN 101054242 B CN101054242 B CN 101054242B CN 200710065134 A CN200710065134 A CN 200710065134A CN 101054242 B CN101054242 B CN 101054242B
- Authority
- CN
- China
- Prior art keywords
- planococcus
- sewage
- resistant
- psychrotoleratus
- cold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241001643373 Planococcus psychrotoleratus Species 0.000 title claims abstract description 58
- 239000010865 sewage Substances 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 claims abstract description 36
- 238000012545 processing Methods 0.000 claims abstract description 15
- 241000193804 Planococcus <bacterium> Species 0.000 claims description 50
- 239000002054 inoculum Substances 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 239000008107 starch Substances 0.000 abstract description 37
- 229920002472 Starch Polymers 0.000 abstract description 27
- 235000019698 starch Nutrition 0.000 abstract description 27
- 239000002351 wastewater Substances 0.000 abstract description 7
- 239000000356 contaminant Substances 0.000 abstract description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 abstract 1
- 108010000912 Egg Proteins Proteins 0.000 abstract 1
- 235000014103 egg white Nutrition 0.000 abstract 1
- 210000000969 egg white Anatomy 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 30
- 239000010840 domestic wastewater Substances 0.000 description 21
- 230000001580 bacterial effect Effects 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 244000005700 microbiome Species 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 229910017053 inorganic salt Inorganic materials 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 238000006731 degradation reaction Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 239000012266 salt solution Substances 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000003344 environmental pollutant Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 102000004882 Lipase Human genes 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- ZFRKQXVRDFCRJG-UHFFFAOYSA-N skatole Chemical compound C1=CC=C2C(C)=CNC2=C1 ZFRKQXVRDFCRJG-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 238000009010 Bradford assay Methods 0.000 description 1
- 108010076119 Caseins Proteins 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- -1 albumen Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011449 brick Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- 108010079058 casein hydrolysate Proteins 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000002478 diastatic effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000000407 epitaxy Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000011133 lead Substances 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229940074386 skatole Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a applications of a strain of Planococcus psychrotoleratus in low temperature sewage disposal, which is the Planococcus psychrotoleratus AS 1.2722 applied in low temperature sewage disposal. In the low temperature sewage disposal method, Planococcus psychrotoleratus AS 1.2722 is inoculated in sewage, cultured in 10 DEG C to 25 DEG C. The invention utilizes the Planococcus psychrotoleratus AS 1.2722 under lower temperature, may efficiently remove BOD, COD, egg white, fat and the starch in sewage, has very high application value in biological processing which low temperature sewage/waste water (such as sewage) containing this kind of contaminant.
Description
Technical field
The present invention relates to the application of a planococcus psychrotoleratus in low-temperature sewage is processed, the particularly application of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 in low-temperature sewage is processed.
Background technology
Along with developing rapidly of China's work, agricultural, problem of environmental pollution becomes problem very serious and in the urgent need to address in Chinese society evolution day by day, it has not only restricted the Economic development of China and the raising of living standards of the people, and seriously limited the Sustainable development of Chinese society, people ' s health is produced to great threat, according to Chinese Academy of Sciences's initial estimate, the current loss being caused by environmental pollution and ecological damage of China has accounted for 15% of GDP total value, environmental problem, has become the demon who engulfs economic gain.
Environment protection has become a major issue in current international relations, economic cooperation, also day by day seriously affects the Sustainable development of Chinese national economy.In China's Economic development in the past few decades, owing to having ignored developing environment protection, environmental aspect is very severe at present.Though taked in recent years a large amount of measure of control, environmental quality downward trend is still continuing.The quantity discharged of waste water is also increasing, and within 2005, national wastewater emission amount is 45,000,000,000 tons; The first half of the year in 2006,689.6 ten thousand tons of National Chemical oxygen requirement (COD) total emission volumns, increase by 3.7% on a year-on-year basis; Sulfurous gas (SO
2) 1274.6 ten thousand tons of total emission volumns, increase by 4.2% on a year-on-year basis." current total emissions of major pollutants reduction task is very arduous.For this reason; each department, all departments will further strengthen environmental protection responsibility sense, the sense of urgency; in strict accordance with State Council's requirement; fully implement a scientific outlook on development; actively promote industry restructuring and the change in the style of economic growth; the dynamics that strengthens Pollution abatement, environmental enhancement supervision and management, guarantees the realization of Eleventh Five-Year Plan total amount reduction target ".The wastewater treatment rate approximately 70% of current China; sewage disposal compliance rate is lower; even the Guangdong Province of doing preferably in sewage disposal; it processes compliance rate is only also 70% left and right; other vast regions of China; northern area particularly, about 5-15 ℃, its processing efficiency also will reduce more than 30% the sewage water temperature in winter (during fingering biological treatment system) than usual.
Sanitary sewage is the sewage from family, office, business and urban public utilities and urban runoff.Fresh municipal effluent gradually old and corruption declines dissolved oxygen content, occurs anaerobic degradation reaction, produces hydrogen sulfide, mercaptan, indoles and skatole, makes glassware for drinking water foul smelling.The composition of sanitary sewage 99% is water, and solid impurity, less than 1%, is mostly non-toxic substance, and wherein inorganic salt have prussiate, vitriol, phosphoric acid salt, ammonium salt, nitrite, nitrate and some heavy carbonic pave floor with bricks, stones etc.s etc.; Organic substance has Mierocrystalline cellulose, starch, carbohydrate, fat, protein and urea etc., also has in addition various washing composition and trace metal, and the latter is as zinc, copper, chromium, manganese, nickel and lead etc.; In sanitary sewage, also contain a large amount of miscellaneous bacterias, be mainly coliform.In sanitary sewage, the content of the phosphorus of nitrogen is higher in addition, is mainly derived from business sewage, urban ground runoff and ight soil, washing composition etc.
City domestic sewage is processed and since the Industrial Revolution, had more and more been subject to people's attention before 200 years.Treatment rate of domestic sewage has become an important symbol that whether civilized area is.Over nearly 200 years, municipal sewage treatment develops into and utilizes various modern techniquies, deeply treating wastewater from original processing naturally, simple coagulation, and reuse.Treatment process is also from traditional activated sludge process, development of oxidation ditch to A/O, A
2the kinds of processes such as/O, AB, SBR (comprising CCAS technique), to reach different water outlet requirements.The sewage disposal of decades is put into practice and is shown, Environmental Biotechnology is in wastewater treatment, to have the treatment technology of superiority most, is also current the most frequently used treatment technology, and its most basic advantage is " it is pollutent technology for eliminating rather than separated from contaminants technology "
(Biotechnology Newswatch，August 16，1993)。Thereby be subject to the great attention of countries in the world.It is worth mentioning that, nearly all city domestic sewage is processed all to adopt and be take the treatment process that microbiological treatment technology is core.
In the biologic treating technique of waste water, no matter be suspension cell method, or activated sludge process or biomembrance process or add biological einforcement method or the additive method of Black Liquor with Efficient Bacteria, its key core is the microorganism in treatment system, comprises anaerobic grain sludge and aerobic activated sludge, microbial film and the efficient degrading bacteria adding.Microorganism in currently used various biological treatment system is all some normal temperature microorganisms, and the life quintessence of normal temperature microorganism has determined that their optimal temperature scopes are conventionally between 20-35 ℃, when temperature is reduced to 15 ℃ or following, its vital movement, comprise growth and breeding and metabolic activity, be subject to strongly inhibited, be also just difficult to effective degradation of contaminant, so under winter low temperature condition, the reduction of processing efficiency is the inevitable outcome of current biologic treating technique.
At environmental situation, increasingly sharpen simultaneously discharging standards also increasingly stringent in the situation that, although the problem that under cold condition, sewage treating efficiency is low has caused, pay close attention to and pay attention to, at present good solution idea and method not also.In order to improve the efficiency of the Sewage treatment systems under winter low temperature condition, the method adopting at present both at home and abroad, except adding gentle Insulation, there is no additive method; And add the investment that gentle Insulation has not only increased equipment, and improved operational and administrative expenses, be to be mainly difficult to carry out in large-scale sewage work.The problem reducing in order to solve winter low temperature processing efficiency, the most basic outlet is that research and development still has the psychrophile bacterial classification of efficient degradation pollutent ability under cold condition.
Microbiological current research and development show, low temperature environment is as the ground such as the South Pole, the arctic, glacier a large amount of microorganism that also surviving, they still can carry out growth faster under cold condition, and psychrophile can be divided into two large classes according to its growth temperature: (1) psychrophilic bacteria (psychrophiles) and psychrotroph (psychrotrophs).Psychrophilic bacteria refers to growth temperature range at-2-20 ℃, the microorganism of 10 ℃ of left and right of optimum growth temperature, and this quasi-microorganism still has Fast Growth breeding, maintains the ability of high reactivity metaboilic level under 5-15 ℃ of condition; Psychrotroph refers to growth temperature range at 7-30 ℃, the microorganism of 20 ℃ of left and right of optimum growth temperature, and this quasi-microorganism still has Fast Growth breeding, maintains the ability of high reactivity metaboilic level under 15-25 ℃ of condition.Sewage/water wasting in winter water temperature of the vast northern area of these microbial growth temperature ranges and China matches, and is that a class can develop, valuable water treatment microorganism resource.
Cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722: Gram-negative, it is shaft-like that somatic cells is.Substratum requirement, LB substratum: peptone 10g, yeast extract 5g, sodium-chlor 10g, adding distil water is settled to 1000ml after dissolving, pH nature.This bacterial strain has the 16SrRNA gene order as shown in sequence in sequence table 1.
Summary of the invention
The object of this invention is to provide the application of a planococcus psychrotoleratus in low-temperature sewage is processed, i.e. the application of cold-resistant planococcus (Planococcus psychrotolera tus) AS 1.2722 in low-temperature sewage is processed.
The present invention also provides the method for utilizing cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 to carry out low-temperature sewage processing.
The method that low-temperature sewage provided by the present invention is processed is to utilize cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, under cold condition, carry out sewage disposal, concrete grammar is that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 is inoculated in sewage and is cultivated at 10 ℃ to 25 ℃.
The temperature that described cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 cultivates in sewage is preferably 18-22 ℃.
Described cold-resistant planococcus (Planococcus psychrotolera tus) AS 1.2722 inoculum densities are not less than 10
5, in order to save cost, dropping into, the scope of described inoculum density can be 10
5-10
8cfu/mL, is preferably 10
7cfu/mL.
Described sewage can be sanitary sewage, starch factory sewage, holds fowl aquaculture wastewater or slaughterhouse sewage, is preferably sanitary sewage.
The 16S rRNA gene of described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 has the nucleotide sequence as shown in sequence in sequence table 1.
The present invention utilizes the feature of cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 tolerance low-temperature epitaxy environment, using it for low-temperature sewage processes, experimental results show that, by in cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 access sanitary sewages, at lower temperature, (10-25 degree Celsius) this strain growth is good, can effectively remove starch in sewage, albumen, fat and BOD, COD, in the biological treatment of the low-temperature sewage/waste water (as sanitary sewage) containing this pollutant, there is very high using value.
Accompanying drawing explanation
Fig. 1 is the growth curve of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722
Embodiment
Method in following embodiment, if no special instructions, is ordinary method.
Percentage composition in following embodiment, if no special instructions, is quality percentage composition.
The cultivation of embodiment 1, cold-resistant planococcus (Planococcus psychrotolera tus) AS 1.2722 and growth curve are measured
With aseptic technique, corresponding bacterial classification is received in substratum, cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 is pressed to 10
4the amount of cfu/mL is inoculated in 200ml LB substratum (peptone 10g is housed, yeast extract 5g, sodium-chlor 10g, after adding distil water dissolves, be settled to 1000ml, 121 ℃, 30min steam sterilizing) the Erlenmeyer flask of 500ml in, after inoculation, on low temperature shaking table, controlling temperature is 12 ℃, rotating speed 150rpm, and the turbidity (OD of culture is measured in interval certain hour sampling (5mL)
600), draw growth curve.As shown in Figure 1, result shows result, and at 12 ℃, approximately 40 hours delayed growth phases of southern cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, cultivation that need to approximately 80 hours can reach maximum growth amount, OD
600can reach more than 2.0 (Fig. 1).
The degraded enzyme of embodiment 2, cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722
According to sanitary sewage, take the feature that starch, albumen and fat is principal pollutant, qualitative test the relevant enzymes of cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722.Diastatic mensuration adopts starch dull and stereotyped---and IKI method, if bacterial strain can form the transparent circle that is greater than bacterium colony on starch flat board, contains amylase at this bacterial strain of explanation; The mensuration of proteolytic enzyme adopts casein hydrolysis method, if bacterial strain can discharge amino acid by caseinhydrolysate, illustrates that this bacterial strain has the activity of proteolytic enzyme; The mensuration of lipase adopts dull and stereotyped---and tween method, if bacterial strain can form transparent circle on tween flat board, illustrates that this bacterial strain contains lipase.Result shows, cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 has proteolytic enzyme, amylase and lipase, and the existence of these enzymes, is conducive to the function and efficacy that bacterial strain is brought into play its degradation of contaminant in treatment system.
The degraded of embodiment 3, cold-resistant planococcus (Planococcus psychrotolera tus) 1.2722 pairs of starch of AS
Preparation starch culture-medium: Na
2hPO
4, 2.0g; KH
2pO
4, 1.0g; MgSO
47H
2o, 0.1g; (NH
4)
2sO
4, 0.5g; NaCl, 10g; Zulkovsky starch, 6.8g; Inorganic salt solution, 5mL; H
2o, 1000mL; PH7.2.Described inorganic salt solution is: EDTA 0.5g, ZnSO
47H
2o 0.22g, CaCl
20.055g, MnCl
24H
2o0.051g, FeSO
47H
2o 0.0499g, (NH
4)
6mo
7o
244H
2o 0.011g, CuSO
45H
2o 0.0157g, CoCl
26H
2o 0.0161g, water is settled to 1000mL, pH6.0.Substratum is sub-packed in 100mL in the triangular flask of 250mL, 121 ℃ of sterilizing 30min.
The cooling rear a small amount of cold-resistant planococcus of transfering loop picking (Planococcus psychrotoleratus) AS1.2722 that uses is by 10
4the amount of cfu/mL is inoculated in above-mentioned starch culture-medium, cultivates (150rpm) with 12 ℃ of shaking tables, and irregularly strain growth amount and starch residual content are measured in sampling (5mL).The mensuration of strain growth represents with the turbidity at wavelength 600nm place; The mensuration of starch adopts IKI colour developing spectra photometric method.The result of cultivating 10 days is as shown in table 1.Result shows, 1.2722 pairs of starch of cold-resistant planococcus (Planococcuspsychrotoleratus) AS all have good degradation capability, through the cultivations of 10 days, the degradation rate of starch is reached more than 66%, completely can be for the processing of starch in sewage.
The degraded of 1.2722 pairs of starch of the cold-resistant planococcus of table 1. (Planococcus psychrotoleratus) AS
| Bacterial strain | Strain growth (OD 600) | Starch residual content (g/L) | Starch clearance (%) |
| Cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 | 0.57 | 2.3 | 66.2 |
The treatment effect experiment of embodiment 4,1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcus psychrotoleratus) AS
According to sanitary sewage, take starch, albumen and fat as principal pollutant, and its COD content is about the feature that 300mg/L and C: N: P is about 100: 5: 1, preparation simulated domestic wastewater: with glucose+starch (each 50%), NH
4cl+ peptone (each 50%), K
2hPO
4and the inorganic salt solution of 5mL/L is mixed with the simulated domestic wastewater that COD is 300mg/L (glucose 0.15g/L, starch 0.15g/L, yeast powder 0.01g/L, NH
4cl 0.01g/L, KH2PO4 0.005g/L, inorganic salt solution 5mL/L).Wherein, inorganic salt solution is with the inorganic salt solution of embodiment 3.This simulated domestic wastewater COD is 300 mg/L, and BOD content is that 285mg/L, starch content are 150mg/L, and protein content is 10mg/L.
The simulation test that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 is carried out to sanitary sewage disposal, concrete steps are as described below:
With cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 of a little slant culture of inoculating needle picking, be inoculated in the 250mL triangular flask containing the above-mentioned simulated domestic wastewater of 100mL, 12 ℃ of shaking tables are cultivated, and irregularly the growing state of bacterial strain in simulated domestic wastewater and the removal effect of COD are measured in sampling (5mL).The mensuration of strain growth represents with the turbidity at wavelength 600nm place; The mensuration of COD adopts the mensuration of National Standard Method (GB11914-89), starch to adopt the mensuration of IKI method, albumen to adopt Bradford method, BOD
5mensuration adopt 5 days standard laws to measure the indices before and after cultivating.Cultivate shown in the detected result table 2 after 3 days, result shows, under the condition of 12 ℃, cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 can adapt to simulated domestic wastewater better, and consider that this is under the very little condition of inoculum size, only to cultivate the result obtaining for 3 days, if the reality of being applied to, the in the situation that of large inoculum size, processing power will be further enhanced.
1.2722 pairs of simulation lives of the cold-resistant planococcus of table 2. (Planococcus psychrotoleratus) AS
The treatment effect of sewage.
| Index | Residual content (mg/L) | Clearance (%) |
| Strain growth (OD 600) COD BOD 5Starch albumen | 0.017 217 206 130 8.5 | 27.7 29 13.3 15 |
The treatment effect experiment of embodiment 5, large 1.2722 pairs of simulated domestic wastewaters of the cold-resistant planococcus of inoculum size (Planococcus psychrotoleratus) AS
According to the formula described in embodiment 4 and composition preparation simulated domestic wastewater, with 1.5 * 10
8the inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 to this simulated domestic wastewater, according to the culture condition described in embodiment 4, shaking table was cultivated after 3 days, adopted as the measuring method of embodiment 4, had measured COD, BOD before and after cultivating
5, starch, protein content, result is as shown in table 4, the in the situation that of large inoculum size, 1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcus psychrotoleratus) AS have good treatment effect, and the clearance of every pollutent index can reach more than 90% (except albumen).
1.2722 pairs of simulation lives of the cold-resistant planococcus of table 4. (Planococcus psychrotoleratus) AS
The treatment effect of sewage
| Index | Residual content (mg/L) | Clearance (%) |
| Strain growth (OD 600) COD BOD 5Starch albumen | 0.121 13 9 15 1.5 | 95.7 97 90 85 |
The processing experiment of 1.2722 pairs of simulated domestic wastewaters of (Planococcus psychrotoleratus) AS of cold-resistant planococcus under embodiment 6, differing temps
According to the formula described in embodiment 4 and composition preparation simulated domestic wastewater, with 10
6the inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722, at different temperature, test the processing of 1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcus psychrotoleratus) AS, cultivating the changing conditions of measuring afterwards the increment of bacterial strain and the COD of simulated domestic wastewater, BOD, starch and albumen for 3 days.Result is as shown in table 4, and result shows, cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 8-25 ℃ all can treatment of simulated sanitary sewage, preferred range is 12-18 ℃.
Cold-resistant planococcus under table 4. differing temps (Planococcus psychrotoleratus)
The processing of 1.2722 pairs of simulated domestic wastewaters of AS
| Temperature (℃) | Index | Residual content (mg/L) | Clearance (%) |
| 8 | Growth (OD 600) COD BOD 5Starch albumen | 0.01 252 240 141 9.5 | 16 17 6 5 |
| 12 | Growth (OD 600) COD BOD 5Starch albumen | 0.017 217 206 130 8.5 | 27.7 29 13.3 15 |
| 18 | Growth (OD 600) COD BOD 5Starch albumen | 0.019 210 201 128 8.3 | 30 30.7 14.7 17 |
| 25 | Growth (OD 600) COD BOD 5Starch albumen | 0.015 225 216 135 9 | 25 25.5 10 10 |
The processing experiment of the cold-resistant planococcus of embodiment 7 different vaccination amount (Planococcus psychrotoleratus) AS1.2722 to simulated domestic wastewater
According to the formula described in embodiment 4 and composition preparation simulated domestic wastewater, with 10
5cfu/mL, 10
6cfu/mL, 10
7cfu/mL and 10
8the inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, at 12 ℃ of temperature, test the processing of 1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcuspsychrotoleratus) AS, cultivating the changing conditions of measuring afterwards the increment of bacterial strain and the COD of simulated domestic wastewater, BOD, starch and albumen for 3 days, result is as shown in table 6.The preferred inoculum size scope that shows cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 treatment of simulated sanitary sewages is 10
7-10
8.
Cold-resistant planococcus under table 6 different vaccination amount (Planococcus psychrotoleratus)
The processing of 1.2722 pairs of simulated domestic wastewaters of AS
| Inoculum size | Index | Residual content (mg/L) | Clearance (%) |
| 10 5cfu/mL | Growth (OD 600) COD BOD 5Starch albumen | 0.10 65 55 35 4.5 | 78.3 81 76.7 55 |
| 10 6cfu/mL | Growth (OD 600) COD BOD 5Starch albumen | 0.113 18 12 16 1.5 | 94 96 89.3 85 |
| 10 7cfu/mL | Growth (OD 600) COD BOD 5Starch albumen | 0.121 13 9 15 1.5 | 95.7 97 90 85 |
| 10 8cfu/mL | Growth (OD 600) COD BOD 5Starch albumen | 0.123 12 9 12 1.5 | 96 97 92 85 |
Sequence table
<160>1
<210>1
<211>1517
<212>DNA
The cold-resistant planococcus of <213> (Planococcus psychrotoleratus)
<400>1
Claims (8)
1. the application of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 in low-temperature sewage is processed.
2. the method for a low-temperature sewage processing is that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 is inoculated in sewage and is cultivated at 10 ℃ to 25 ℃.
3. method according to claim 2, is characterized in that: described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 optimum temperature range of disposing of sewage is 12-18 ℃.
4. method according to claim 3, is characterized in that: described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 inoculum densities are not less than 10
5cfu/mL.
5. method according to claim 4, is characterized in that: described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 inoculum densities are 10
5-10
8cfu/mL.
6. method according to claim 5, is characterized in that: described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 inoculum densities are 10
7-10
8cfu/mL.
7. according to the method described in claim 5 or 6, it is characterized in that: described sewage is sanitary sewage.
8. method according to claim 7, is characterized in that: the 16S rRNA gene of described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 has the nucleotide sequence as shown in sequence in sequence table 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710065134.1A CN101054242B (en) | 2007-04-05 | 2007-04-05 | Application of planococcus psychrotoleratus in treating sewage at low temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710065134.1A CN101054242B (en) | 2007-04-05 | 2007-04-05 | Application of planococcus psychrotoleratus in treating sewage at low temperature |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101054242A CN101054242A (en) | 2007-10-17 |
| CN101054242B true CN101054242B (en) | 2014-12-10 |
Family
ID=38794323
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200710065134.1A Expired - Fee Related CN101054242B (en) | 2007-04-05 | 2007-04-05 | Application of planococcus psychrotoleratus in treating sewage at low temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101054242B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101666793B1 (en) * | 2015-02-27 | 2016-10-18 | 전남대학교산학협력단 | Novel plasmid pPla isolated from Planococcus sp. PAMC 21323 |
| CN104818225A (en) * | 2015-03-10 | 2015-08-05 | 天津科技大学 | Low temperature protease production strain, low temperature protease produced by the same and gene of enzyme |
| CN108611291A (en) * | 2018-04-08 | 2018-10-02 | 哈尔滨工业大学（威海） | One plant of salt tolerance planococcus and its application |
| CN109019875B (en) * | 2018-09-10 | 2021-05-07 | 黑龙江百盈环保工程有限公司 | Method for improving removal effect of phosphorus and nitrogen in low-temperature sewage |
| CN109133518B (en) * | 2018-09-19 | 2021-07-30 | 邵春彦 | Improved method for improving domestic sewage treatment efficiency under low-temperature condition |
| CN115838633A (en) * | 2022-08-02 | 2023-03-24 | 定西市食品检验检测中心 | Geotrichum candidum and application thereof in low-temperature treatment of potato starch sewage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308670A (en) * | 1998-01-16 | 2001-08-15 | 第一制糖株式会社 | Microorganisms and preparation for disposing of organic wastewater |
| CN1384065A (en) * | 2001-04-28 | 2002-12-11 | 沈阳碧水蓝天环境科技有限公司 | Biological treating process of high-concentration organic waste water |
| CN1509991A (en) * | 2002-12-24 | 2004-07-07 | 中国科学院微生物研究所 | Low temperature nitrobacter agent and use thereof |
-
2007
- 2007-04-05 CN CN200710065134.1A patent/CN101054242B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308670A (en) * | 1998-01-16 | 2001-08-15 | 第一制糖株式会社 | Microorganisms and preparation for disposing of organic wastewater |
| CN1384065A (en) * | 2001-04-28 | 2002-12-11 | 沈阳碧水蓝天环境科技有限公司 | Biological treating process of high-concentration organic waste water |
| CN1509991A (en) * | 2002-12-24 | 2004-07-07 | 中国科学院微生物研究所 | Low temperature nitrobacter agent and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王丹 等，.复合式生物池处理低温污水试验研究.《低温建筑技术》.2006,(第4期), * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101054242A (en) | 2007-10-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102061276A (en) | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof | |
| CN101054242B (en) | Application of planococcus psychrotoleratus in treating sewage at low temperature | |
| CN103103153B (en) | Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof | |
| CN103540544B (en) | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof | |
| CN101386823B (en) | Special effect anaerobic denitrifying bacterium and waste water processing method using thereof | |
| CN103114062B (en) | Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof | |
| CN102061277A (en) | Acinetobacter johnsonii strain for biological denitrification under low temperature and application thereof | |
| Zuo et al. | Microbial community structure analyses and cultivable denitrifier isolation of Myriophyllum aquaticum constructed wetland under low C/N ratio | |
| CN104862260A (en) | Arthrobacter with aerobic denitrification capability and application thereof | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| Lu et al. | A review of ammonia-oxidizing archaea and anaerobic ammonia-oxidizing bacteria in the aquaculture pond environment in China | |
| CN103275908B (en) | Low-temperature denitrification pseudomonas fluorescens | |
| CN104611279B (en) | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes | |
| Lahiri et al. | Biogeochemical cycling bacteria and nutrient dynamics in waste stabilization pond system | |
| CN114395505B (en) | Low-temperature denitrifying bacterium and application thereof | |
| CN101386822B (en) | Special effect phosphate accumulating organisms and waste water processing method using thereof | |
| CN101029298A (en) | Production of efficient microbe bacteria combing agent | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature | |
| CN109609407B (en) | Thermophilic microorganism strain for in-situ sludge reduction and application thereof | |
| CN104388342B (en) | A kind of short distance nitration bacterium pseudomonad and application | |
| CN104694435A (en) | Shinella with triazole degradation function and application thereof | |
| CN104609541B (en) | The direct biochemical processing method of a kind of decanedioic acid not desalination of waste water | |
| CN113215027A (en) | Alcaligenes aquaticum AS1 and application thereof in sewage treatment | |
| CN101054243A (en) | Application of micrococcus antarcticus in treating sewage at low temperature | |
| CN110317737A (en) | Compound EM microorganism formulation, preparation method and application method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20071017 |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 |