CN110317737A - Compound EM microorganism formulation, preparation method and application method - Google Patents
Compound EM microorganism formulation, preparation method and application method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention discloses a kind of methods of EM strain preparation and black and odorous water mud decrement, the compound EM microorganism formulation includes following strain: saccharomycete, actinomyces, Bacillus acidi lactici, Plesiomonas, pseudomonas, enterobacteria category, Bacillus cercus, bacillus subtilis, acinetobacter calcoaceticus, brevibacterium, Micrococcus, Flavobacterium, Plesiomonas, streptococcus, Klebsiella, Arthrobacter, bacillus, streptococcus, pseudomonas aeruginosa, Pseudomonas fluorescens, Nitrobacter, Nitrosococcus, Nitromonas, Alcaligenes.The preparation method includes: that each strain in compound EM microorganism formulation is inoculated into the fermentor equipped with fermentation medium.The application method, which is included in black and odorous water, adds compound EM microorganism formulation.This invention simplifies treatment process, treatment effect is not only increased, and cost is relatively low, is able to achieve effective abatement of black and odorous water sludge.
Description
Technical field
The present invention relates to the biologic treating technique fields of sewage, more particularly to a kind of compound EM microorganism formulation, preparation
Method and application method.
Background technique
With the quickening of urbanization process, the water eutrophications problem such as rivers and lakes is got worse, the water quality of water body
Situation worse and worse, if things go on like this just leads to black smelly phenomenon, and the ecological environment of water body is destroyed significantly, and so far caused people occupies
The deterioration of environment is also got worse.The improvement of polluted water body, which has become governments at all levels, improves the vital task of human settlement environment in city.
Traditionally, the reparation of water body is all by the way of changing water, water transfer, and its essence is the transfer of pollutant and dilutions, only will be dirty
It contaminates object and has been transferred to another river from Yi Tiaohe, essential problem is not resolved.
When Organic Pollutants In Water too high levels, under the action of aerobic microbiological, organic matter decomposition can largely disappear
Oxygen in water consumption makes water body be converted to anoxic or anaerobic state.Under anoxic and anaerobic condition, the gold such as iron, manganese in water body
The sulphion belonged in ion and water forms the compounds such as ferrous sulfide, manganese sulfide.Suspended particle absorbing control ferrous sulfide, manganese sulfide
Deng causing water body blackening；Organic matter corruption is decomposed, and the stenches such as ammonia, hydrogen sulfide, mercaptan, thioether, organic amine and organic acid are generated
Substance causes water body to foul.
The principal element that being not difficult to analyze as a result, influences black and odorous water has: organic pollutant concentration, nutrient concentrations,
Pollute the time (time through going through after pollution is formed), hydraulics, temperature condition etc..So to administer black and odorous water, first have to
Artificial aeration's oxygenation, changes anaerobic environment, and secondary help water body establishes powerful self-purification capacity, makes the immune of water body itself
System.
The decrement research for the remaining black and odorous water sludge that biological wastewater treatment generates is concentrated mainly on 2 aspects: first is that right
The remaining black and odorous water sludge generated is handled (microwave treatment etc.), is again returned to after so that black and odorous water sludge is liquefied black
Smelly water body such as lake river further processes；Second is that reducing black and odorous water sludge yield during biological wastewater treatment
(enhanced biological metabolism, uncoupling etc.).
Current black and odorous water Sludge Reduction Technologies are based primarily upon three classes action principle, one kind be based on cell dissolution (or
Decompose) the black and odorous water Sludge Reduction Technologies of a recessive growth, mechanical, chemistry first is carried out to wet black and odorous water sludge, heat one is changed
It learns, biological oxidation, is crushed and decomposes black and odorous water sludge wadding body, promote microbial cell dissolution, make the organic carbon in organism
Substrate and repetition metabolism for microorganism, that is, the recessive growth for utilizing cell lysates to carry out；Second class principle is to increase
The quantity of bacterium predator in adding system is that the black and odorous water sludge that the food chain principle in imitated natural ecosystem carries out subtracts
Quantification technique；It is that uncoupling metabolism is promoted using chemistry or biological method there are also one kind, energy leakage is caused, to make biological life
Long efficiency decline.
Low etc. is reported in the black and odorous water sludge system of laboratory scale, is given birth to after adding people's p-nitrophenol (PNP)
The generation of object amount can reduce 49%.Strand et al. has found black and odorous water sludge by long-term domestication and adapts to, and finally makes uncoupling
Agent can not reduce black and odorous water sludge yield.Abbassi etc. carries out high concentration dissolved oxygen and black and odorous water sludge yield relationship
Lab scale research, the results showed that the deep diffusion of oxygen can be improved in the concentration for increasing oxygen, so that in black and odorous water sludge wadding body
The expansion of portion's aerobic region, unhydrolysed biomass can be by aerobic degradation, to make black and odorous water sludge in wadding body
Amount is reduced.CoPP and Dofd report, compared with aerobic condition, black and odorous water sludge growth rate reduces under anaerobic
38%.Peng etc. is also reported in biosystem when anaerobic denitrifying occurs, and black and odorous water sludge yield is reduced.
Westggartll et al. reports the insertion anaerobic digestion section in the smelly water sludge technique of efficient black for the first time can reduce the surplus of half
Remaining black and odorous water sludge yield.Mason and Hame is had studied the most suitable of cell lysates and is disappeared using mixing thermophilic bacteria population
Change condition.The black and odorous water sludge yield range of black and odorous water sludge aerobic thermophilic digestion is 0.05-0.13kgTs/kgCOD, so
And its investment and operating cost are high.Neis et al. compares ultrasonication black and odorous water sludge zoogloea under different frequency and deconstructs
Efficiency.
The it is proposeds such as the H.Yasui of Japan utilize the strong oxidizer of ozone, occur with the compound in black and odorous water sludge straight
It connects or indirect reaction, destruction cell wall releases cytoplasm, while also resolving into macromolecular not soluble in water and being dissolved in the small of water
Molecular fragments carry out black and odorous water mud decrement.Svensson et al. system research supercritical water oxidation black and odorous water sludge
Method, at 374 DEG C of temperature and the pressure of 22.IMPa, ingredient is all organic uniformly, in same nature black and odorous water sludge
Object almost all is degraded, treated black and odorous water sludge can reuse again, basically reach no black and odorous water mud discharging.But
Supercritical water oxidation method needs high temperature, condition of high voltage, more demanding to the anticorrosion of equipment.One u s company BIOCOPE report
Processed conventionally dissolved oxygen can be reduced to 0.8~1.2mg/l by the enzyme solutions that road utilizes them to develop, while MLss is 4500-
8500 mg/l, black and odorous water sludge age are 30-70d, without operation problem, reduce black and odorous water sludge yield 50%, eliminate
Black and odorous water sludge digestion equipment.
The submerged biofilm New Technology about Wastewater Treatment of the exploitations such as HarBin Building University king treasured chastity, using fixed carrier
Filler increases the quantity of protozoan and metazoa in aeration tank, effectively reduces remaining black and odorous water sludge
Yield, it is only the 1/10 1 1/5 of conventional black and odorous water sludge that lab scale, which determines its remaining black and odorous water sludge yield,.
The lab scale research that Ratsak etc. has carried out infusorian using two-stage method, preyed on bacterium, finds its organism yield ratio without fibre
Caterpillar predation reduces 12%~43%.One basic environment microorganism company, Switzerland COHO Hydro CleanSA utilizes outer throwing
The different types of sewage of microbiological treatment, black and odorous water can be reduced 16% hyperplasia black and odorous water sludge quantity.
Shijiazhuang Development Zone Desai Chemical Co., Ltd has invented a kind of for the compound of materialization black and odorous water Treatment of Sludge
EM microorganism formulation, preparation include the microorganisms such as photosynthetic bacteria group, lactobacillus, saccharomyces cerevisiae, Gram positive actinomycetes group
Flora, improves the physical and chemical state of black and odorous water sludge, and then promotes materialization black and odorous water sludge reduction, stabilisation and harmless
Change.The PM-I black and odorous water mud decrement microorganism formulation of Shanghai Zhenwei Environment Engineering Co., Ltd.'s invention is a kind of for city
The compound EM microorganism formulation of black and odorous water mud decrement in sanitary sewage and Industrial Wastewater Treatment, wherein containing photosynthetic bacteria
Useful and harmless micro- of a variety of pairs of people, animal, the plants such as group, yeast flora, lactobacillus, Propionibacterium group, fungi, actinomycete group
Biology.
Technically not perfect and mature or adaptation range is small for majority black and odorous water sludge Sources decreasing technology at present,
Or do not have economic serviceability, fail preferably in black and odorous water application, there is an urgent need to develop the good new skills of efficient, adaptability
Art.Black and odorous water sludge Sources decreasing is carried out using microorganism, gives full play to the efficient of microorganism, low consumption, multipotency and self-reproduction
Characteristic, develop it is a kind of efficiently, low consumption, energy saving black and odorous water sludge Sources decreasing technology and realize industrial application, will significantly
Promote black and odorous water processing in China's to realize sustainable development, preferably protects the saving and utilization of environment and water resource.
Summary of the invention
Based on this, it is necessary to aiming at the problem that how to improve the treatment effeciency of black and odorous water, reduce processing cost, provide one
The compound EM microorganism formulation of kind, preparation method and application method.
A kind of compound EM microorganism formulation, including following strain and mass percent:
Saccharomycete 6%-10%, actinomyces 5%-11%, Bacillus acidi lactici 8%-12%, Plesiomonas 1%-4%, pseudomonas 1%-
5%, enterobacteria belongs to 1%-4%, Bacillus cercus 2%-7%, bacillus subtilis 6%-10%, acinetobacter calcoaceticus 2%-7%, short
Bacillus 1%-4%, Micrococcus 1%-4%, Flavobacterium 1%-5%, Plesiomonas 1%-4%, streptococcus 1%-5%, citric acid
Pseudomonas 1%-5%, Arthrobacter 1%-5%, bacillus 2%-7%, streptococcus 1%-5%, pseudomonas aeruginosa 1%-4%, fluorescence are false single
Born of the same parents bacterium 2%-7%, Nitrobacter 2%-7%, Nitrosococcus 2%-7%, Nitromonas 2%-7%, Alcaligenes 1%-4%.
The EM strain preparation includes following component and mass percent in one of the embodiments: saccharomycete 8% is put
Line Pseudomonas 7%, Bacillus acidi lactici 10%, Plesiomonas 2%, pseudomonas 3%, enterobacteria belong to 2%, Bacillus cercus 5%, withered grass
Bacillus 8%, acinetobacter calcoaceticus 5%, brevibacterium 2%, Micrococcus 2%, Flavobacterium 3%, Plesiomonas 2%, chain
Coccus 3%, Klebsiella 3%, Arthrobacter 3%, bacillus 5%, streptococcus 3%, pseudomonas aeruginosa 2%, fluorescence are false single
Born of the same parents bacterium 5%, Nitrobacter 5%, Nitrosococcus 5%, Nitromonas 5%, Alcaligenes 2%.
A kind of preparation method of compound EM microorganism formulation, including the following steps: will be described in above-mentioned any embodiment
Each strain in compound EM microorganism formulation is inoculated into according to its mass percent equipped with fermentation medium after liquid spreads cultivation
In the fermentor of 1L, inoculum concentration 5%-20%, cultivation temperature is 15-45 DEG C, stirring, when viable count is up to 109When above, it is somebody's turn to do
Compound EM strain preparation.
In one of the embodiments, in the preparation method of the compound EM microorganism formulation, inoculum concentration 5%, cultivation temperature
It is 15 DEG C.
In one of the embodiments, in the preparation method of the compound EM microorganism formulation, inoculum concentration 15%, culture temperature
Degree is 20 DEG C.
The fermentation medium 1L includes following component and mass percent: corn flour in one of the embodiments,
1.0%-4.0%, brown sugar 0.3%-0.7%, beancake powder or fish meal 0.3%-0.7%, CaCO30.3%-0.7%、(NH4)2SO40.08%-
0.12%、K2HPO40.01%-0.05%、MgSO4.7H2O0.01%-0.04% and MnSO4.H2O0.01%-0.04%。
The fermentation medium 1L includes following component and mass percent: corn flour in one of the embodiments,
2.0%, brown sugar 0.5%, beancake powder or fish meal 0.5%, CaCO30.5%、(NH4)2SO40.1%、K2HPO40.03%、
MgSO4.7H2O0.02% and MnSO4.H2O0.02%。
A kind of application method of compound EM microorganism formulation, includes the following steps: to add initial concentration in black and odorous water
For 0.1 ‰ -1.0 ‰ compound EM microorganism formulation as claimed in claim 1 or 2 of black and odorous water processing volume.
0.1 ‰ that initial concentration is black and odorous water processing volume are added in black and odorous water in one of the embodiments,
Compound EM microorganism formulation as claimed in claim 1 or 2.
1.0 ‰ that initial concentration is black and odorous water processing volume are added in black and odorous water in one of the embodiments,
Compound EM microorganism formulation as claimed in claim 1 or 2.
Above-mentioned compound EM microorganism formulation, preparation method and application method, by directly in black and odorous water such as lake river
Compound EM microorganism formulation is added in road, which is the composite microbial bacteria by especially screening and cultivating
Agent, it is real by selectivity and competitive growth and breeding between microbial population original in sewage disposal system after adding
The rearrangement for now planting group relation, forms new dominant microflora, thus increase in sewage disposal system the type of high-effective microorganism and
Concentration, it is suppressed that the growth of unfavorable bacterium and " useless bacterium " improves black and odorous water Sludge Property and metabolism, promotes organic pollutant
It decomposes and dead microbial thallus is constantly decomposed again, the yield of remaining black and odorous water sludge is greatly reduced, subtracts
Few subsequent remaining black and odorous water Treatment of Sludge, realizes from source and controls black and odorous water residue black and odorous water sludge yield.And
And the strengthening process of complex micro organism fungicide only changes the composition and function of the microorganism of black and odorous water sludge, does not change black smelly water
The technique and service condition of body have and well adapt to ability, is the development side of remaining black and odorous water sludge Sources decreasing technology
To.The compound EM microorganism formulation, preparation method and application method simplify treatment process, not only increase treatment effect, and
And cost is relatively low, is able to achieve effective abatement of black and odorous water sludge.
Detailed description of the invention
Fig. 1 is the COD change curve of the water body after the application method of compound EM microorganism formulation of the invention is administered；
Fig. 2 is the ammonia nitrogen change curve of the water body after the application method of compound EM microorganism formulation of the invention is administered；
Fig. 3 is the total phosphorus change curve of the water body after the application method of compound EM microorganism formulation of the invention is administered.
Specific embodiment
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, with reference to the accompanying drawing to the present invention
Specific embodiment be described in detail.Many details are explained in the following description in order to fully understand this hair
It is bright.But the invention can be embodied in many other ways as described herein, those skilled in the art can be not
Similar improvement is done in the case where violating intension of the present invention, therefore the present invention is not limited by the specific embodiments disclosed below.
The present invention provides a kind of compound EM microorganism formulations, which includes following strain and quality
Percentage: saccharomycete 6%-10%, actinomyces 5%-11%, Bacillus acidi lactici 8%-12%, Plesiomonas 1%-4%, pseudomonas
1%-5%, enterobacteria belong to 1%-4%, Bacillus cercus 2%-7%, bacillus subtilis 6%-10%, acinetobacter calcoaceticus 2%-7%,
Brevibacterium 1%-4%, Micrococcus 1%-4%, Flavobacterium 1%-5%, Plesiomonas 1%-4%, streptococcus 1%-5%, Cray primary
Bordetella 1%-5%, Arthrobacter 1%-5%, bacillus 2%-7%, streptococcus 1%-5%, pseudomonas aeruginosa 1%-4%, fluorescence are false
Monad 2%-7%, Nitrobacter 2%-7%, Nitrosococcus 2%-7%, Nitromonas 2%-7%, Alcaligenes 1%-
4%。
Above-mentioned compound EM microorganism formulation, can directly launch in black and odorous water such as lake river, after adding, with dirt
The weight of kind of group relation is realized by selectivity and competitive growth and breeding between original microbial population in water treatment system
Row, forms new dominant microflora, to increase the type and concentration of high-effective microorganism in sewage disposal system, it is suppressed that unfavorable
The growth of bacterium and " useless bacterium " improves black and odorous water Sludge Property and metabolism, promotes organic pollutant to decompose and to dead micro-
Biomass constantly decomposes again, and the yield of remaining black and odorous water sludge is greatly reduced.
The compound EM microorganism formulation includes following component and mass percent: yeast in one of the embodiments,
Bacterium 8%, actinomyces 7%, Bacillus acidi lactici 10%, Plesiomonas 2%, pseudomonas 3%, enterobacteria belong to 2%, Bacillus cercus
5%, bacillus subtilis 8%, acinetobacter calcoaceticus 5%, brevibacterium 2%, Micrococcus 2%, Flavobacterium 3%, Plesiomonas
Belong to 2%, streptococcus 3%, Klebsiella 3%, Arthrobacter 3%, bacillus 5%, streptococcus 3%, pseudomonas aeruginosa 2%, glimmering
Light pseudomonad 5%, Nitrobacter 5%, Nitrosococcus 5%, Nitromonas 5%, Alcaligenes 2%.In this way, passing through
The strain and mass percent for optimizing compound EM microorganism formulation enhance in the compound EM microorganism formulation and sewage disposal system
Competitive power between original microbial population increases its growth and fertility, and further increasing it improves black smelly water
Body Sludge Property and metabolism, the ability for promoting organic pollutant to decompose and constantly decompose again to dead microbial thallus.
The present invention also provides a kind of preparation method of compound EM microorganism formulation, the preparations of the compound EM microorganism formulation
Method includes the following steps: to expand each strain in compound EM microorganism formulation described in above-mentioned any embodiment by liquid
It is inoculated into the fermentor equipped with fermentation medium 1L after training according to its mass percent, inoculum concentration 5%-20%, cultivation temperature
It is 15-45 DEG C, stirring, when viable count is up to 109When above, the compound EM strain preparation is obtained.
In one of the embodiments, in the preparation method of the compound EM microorganism formulation, inoculum concentration 5%, culture temperature
Degree is 15 DEG C.That is, each strain in compound EM microorganism formulation described in above-mentioned any embodiment is passed through liquid
It is inoculated into the fermentor equipped with fermentation medium 1L after spreading cultivation according to its mass percent, inoculum concentration 5%, cultivation temperature is
15 DEG C, stirring guarantees dissolved oxygen using stirring, when viable count is up to 109When above to get arrive the compound EM microorganism formulation,
Claim compound EM strain preparation.
In one of the embodiments, in the preparation method of the compound EM microorganism formulation, inoculum concentration 15%, culture
Temperature is 20 DEG C.That is, each strain in compound EM microorganism formulation described in above-mentioned any embodiment is passed through liquid
Body is inoculated into the fermentor equipped with fermentation medium 1L after spreading cultivation according to its mass percent, inoculum concentration 15%, cultivation temperature
It is 20 DEG C, stirring guarantees dissolved oxygen using stirring, when viable count is up to 109When above to get arrive the compound EM microorganism formulation,
Also referred to as compound EM strain preparation.
The fermentation medium 1L includes following component and mass percent: corn flour in one of the embodiments,
1.0%-4.0%, brown sugar 0.3%-0.7%, beancake powder or fish meal 0.3%-0.7%, CaCO30.3%-0.7%、(NH4)2SO40.08%-
0.12%、K2HPO40.01%-0.05%、MgSO4.7H2O0.01%-0.04% and MnSO4.H2O0.01%-0.04%.That is,
The preparation method of the compound EM microorganism formulation includes the following steps: compound EM microorganism described in above-mentioned any embodiment
Each strain in preparation is inoculated into the fermentor equipped with fermentation medium 1L after liquid spreads cultivation according to its mass percent,
Wherein, fermentation medium 1L includes following component and mass percent: corn flour 1.0%-4.0%, brown sugar 0.3%-0.7%, soya-bean cake
Powder or fish meal 0.3%-0.7%, CaCO30.3%-0.7%、(NH4)2SO40.08%-0.12%、K2HPO40.01%-0.05%、
MgSO4.7H2O0.01%-0.04% and MnSO4.H2O0.01%-0.04%, inoculum concentration 5%-20%, cultivation temperature are 15-45 DEG C,
Stirring guarantees dissolved oxygen using stirring, when viable count is up to 109When above, the compound EM strain preparation is obtained.
The fermentation medium 1L includes following component and mass percent: corn flour in one of the embodiments,
2.0%, brown sugar 0.5%, beancake powder or fish meal 0.5%, CaCO30.5%、(NH4)2SO40.1%、K2HPO40.03%、
MgSO4.7H2O0.02% and MnSO4.H2O0.02%.That is, the preparation method of the compound EM microorganism formulation includes as follows
Step: by each strain in compound EM microorganism formulation described in above-mentioned any embodiment according to its matter after liquid spreads cultivation
Amount percentage is inoculated into the fermentor equipped with fermentation medium 1L, wherein fermentation medium 1L includes following component and quality
Percentage: corn flour 2.0%, brown sugar 0.5%, beancake powder or fish meal 0.5%, CaCO30.5%、(NH4)2SO40.1%、
K2HPO40.03%、MgSO4.7H2O0.02% and MnSO4.H2O0.02%, inoculum concentration 5%-20%, cultivation temperature are 15-45 DEG C,
Stirring guarantees dissolved oxygen using stirring, when viable count is up to 109When above, the compound EM strain preparation is obtained.
Further, fermentation medium 1L is held using molecular sieve culture dish, in order to the existence and procreation of each strain.Tool
Body, which is made using following steps: soft kaolin and distilled water are taken, forms kaolin embryo after mixing,
The kaolin embryo is made to the hollow cuboid soil embryo of a side opening, wherein the mass parts of soft kaolin are 70 parts ~ 85 parts,
The mass parts of distilled water are 15 parts ~ 30 parts, a length of 4cm ~ 16cm of most short side of hollow cuboid soil embryo；By the hollow cuboid soil
Embryo is calcined, and calcined kaolin culture dish is obtained, wherein calcination temperature is 300 degrees Celsius ~ 550 degrees Celsius, when calcining
Between be 5 hours ~ 8 hours；Calcined kaolin culture dish is thrown to prefabricated alkaline solution to impregnate 35 minutes ~ 1 hour, is obtained
Molecular sieve culture dish, wherein the temperature of the kaolin culture dish is 70 degrees Celsius ~ 90 Celsius when being thrown to prefabricated alkaline solution
Degree, prefabricated alkaline solution includes following mass parts each component: 25 parts ~ 30 parts of adult peanut campanulaceae ash, paddy campanulaceae ash 15 parts ~ 33
Part, surplus are water.
In the step of molecular sieve culture dish is made, suitable soft kaolin and distillation can be chosen according to resolution
Water can form kaolin embryo after mixing soft kaolin and distilled water, and kaolin embryo is in half solidification shape body at this time, can be manual
Or machining moulding, the hollow cuboid soil embryo of a side opening is made.Side is made using the kaolin embryo of half solidification
After the hollow cuboid soil embryo of opening, the hollow cuboid soil embryo stability of a side opening is good, in stable condition, is subsequent place
Reason step provides stable environment.
The mass parts of soft kaolin are 70 parts ~ 85 parts, and the mass parts of distilled water are 15 parts ~ 30 parts, hollow cuboid soil
A length of 4cm ~ the 16cm of most short side of embryo.That is, according to resolution, no matter the hollow cuboid of a side opening obtained
How is the size of native embryo, and the proportion of soft kaolin and distilled water is all maintained at the soft kaolin of 70 parts ~ 85 parts mass parts
And the distilled water of 15 parts ~ 30 parts mass parts, in this way, forming kaolin embryo after mixing soft kaolin and distilled water can be in half
Shape body is solidified, in order to which stablizing for subsequent complex microorganism preparations incubation step carries out.
In the step of molecular sieve culture dish is made, the similar mode for smelting ceramics is Celsius using 300 in calcining furnace
~ 550 degrees Celsius of degree, the calcining manners that calcination time is 5 hours ~ 8 hours calcine hollow cuboid soil embryo, are calcined
Kaolin culture dish afterwards.The kaolin culture dish obtained using the calcining manners, the porosity does not drop to minimum, close
The state of maximum value is spent also and reaches, i.e. the kaolin culture dish does not reach sintering state also, at this time the kaolin culture dish knot
Liquid can be penetrated on structure, in order to alkaline solution entrance.
Further, calcined its structure of kaolin culture dish is molded, and the kaolin obtained by calcining step
Culture dish does not reach sintering state, is thrown to prefabricated alkaline solution at this time and impregnated, alkaline solution may make to enter kaolin
In the structure of culture dish, and kaolin alkaline resistance properties is poor, and impregnating by 35 minutes ~ 1 hour short time can destruction height appropriate
The molecular structure of ridge soil culture dish, so that the appearance multi-pore channel of kaolin culture dish, and molecular sieve culture dish is obtained, so that
The air permeability of kaolin culture dish is higher, in favor of the culture of strain.And the kaolin culture dish is 70 Celsius with temperature
~ 90 degrees Celsius of degree is thrown to prefabricated alkaline solution and is impregnated, and may make kaolin culture dish liquid during fast cooling
Body strong adsorption force, molecular structure keep stable state.
The preparation method of the compound EM microorganism formulation further includes following steps in one of the embodiments: this is compound
The preparation method of EM microorganism formulation includes the following steps: will be in compound EM microorganism formulation described in above-mentioned any embodiment
Each strain be inoculated into the molecular sieve culture dish equipped with fermentation medium 1L after liquid spreads cultivation according to its mass percent,
It is placed in fermentor, inoculum concentration 5%-20%, cultivation temperature is 15-45 DEG C, stirring, when viable count is up to 109When above, obtain
The compound EM strain preparation.In this way, using the feature of kaolin alkaline resistance properties difference after firing, by calcined kaolin culture
Ware is thrown to prefabricated alkaline solution and impregnates to obtain multi-pore channel molecular sieve culture dish, and air exchange speed is fast, absorption property is good, energy
A good living environment is provided for subsequent microorganism, is conducive to the existence and procreation of each strain.
The present invention also provides a kind of application methods of compound EM microorganism formulation, include the following steps: in black and odorous water
In add initial concentration be black and odorous water processing volume 0.1 ‰ -1.0 ‰ any of the above-described compound EM as described in the examples it is micro-
Biological agent.
0.1 ‰ that initial concentration is black and odorous water processing volume are added in black and odorous water in one of the embodiments,
Any of the above-described compound EM microorganism formulation as described in the examples.
1.0 ‰ that initial concentration is black and odorous water processing volume are added in black and odorous water in one of the embodiments,
Any of the above-described compound EM microorganism formulation as described in the examples.
The application method of above-mentioned compound EM microorganism formulation, it is multiple by directly being added in black and odorous water such as lake river
EM microorganism formulation is closed, which is the complex micro organism fungicide by especially screening and cultivating, after adding,
It, by selectivity and competitive growth and breeding, realizes kind of a group relation between microbial population original in sewage disposal system
Rearrangement, new dominant microflora is formed, to increase the type and concentration of high-effective microorganism in sewage disposal system, it is suppressed that
The growth of unfavorable bacterium and " useless bacterium " improves black and odorous water Sludge Property and metabolism, and organic pollutant is promoted to decompose and to dead
It dies microbial cells constantly to decompose again, the yield of remaining black and odorous water sludge is greatly reduced, reduce subsequent surplus
Remaining black and odorous water Treatment of Sludge is realized from source and controls black and odorous water residue black and odorous water sludge yield.And composite microbial
The strengthening process of object microbial inoculum only changes the composition and function of the microorganism of black and odorous water sludge, do not change black and odorous water technique and
Service condition has and well adapts to ability, is the developing direction of remaining black and odorous water sludge Sources decreasing technology.Simplify place
Science and engineering skill not only increases treatment effect, and cost is relatively low, is able to achieve effective abatement of black and odorous water sludge.
Specifically carry out the reality of black and odorous water mud decrement using above-mentioned compound EM microorganism formulation in practice below
Example, is illustrated above-mentioned compound EM microorganism formulation.
First example: Yiyang City imperial family, Hunan Province river black and odorous water river engineering segment length about 500m, river mean breadth
For 25m, river two sides, which have been basically completed, cuts dirt, but river water body is muddy, and organic pollutants content is high, water eutrophication
Change seriously, river blue algae bloom has slight bad smell, and water transparency is about 5cm.
By the application method ecological control techniques of above-mentioned compound EM microorganism formulation, transparency is mentioned by original 15cm in 2 months
Up to 60cm, eliminates river blue algae bloom, and water quality reaches IV class water standard of earth's surface.It is by above-mentioned compound EM microorganism below
The application method ecological control techniques of preparation, the photo that prospecting is taken on site in mid-October, 2018, while obtaining local peace
The creek water quality data that ring office provides:
Creek water quality situation before Yiyang City imperial family river black and odorous water is constructed
It can be seen that from above, ammonia nitrogen and total phosphorus content belong to bad five classes water in water body, can also judge from current water body visual condition
Water-outlet body pertains generally to bad five classes water.In addition, being learnt according to site inspection, although river is completed to cut dirt, river two sides have
The overflow pipe of drainage pipeline, when heavy rain, drainage pipeline sewage can also be imported in river.And before interception project, nearby residents are raw
Sewage living is directly discharged into river, and pollutant siltation year in year out deposits organic pollutant, N, P constantly to bed mud, causes
Organic pollutant enrichment in bed mud, by exchanging with overlying water, bed mud will continue to discharge organic pollutant toward water body, if
It handles not in time, water body will gradually deteriorate to black smelly direction.
Second example: accordingly, the application method by above-mentioned compound EM microorganism formulation is the one of core
Change resolution, by pollutant in microorganism conversion, absorption, degradation environment, is aided with the means such as artificial carrier and realizes that water body is net
Change, the recovery of ecological effect, aquatic ecosystem building.
The water quality indicator of bad five classes water system is following (mg/L): COD(Chemical Oxygen Demand, chemical oxygen demand
Amount): 60；BOD(Biochemical Oxygen Demand, biochemical oxygen demand (BOD) or bod): 35；Ammonia nitrogen: 20；Total phosphorus
5.2；SS:60mg/L；The dosage of compound EM microorganism formulation: the 0.1-1.0 ‰ of black and odorous water such as lake river volume is accounted for.
It is black and odorous water such as lake river processing volume that initial concentration is added in black and odorous water such as lake river
The compound EM microorganism formulation of 0.1-1.0 ‰.It is only added for 5 times in 2 months, effluent quality index is (mg/ after system stable operation
L): COD:15；BOD:10；Ammonia nitrogen: 2.4；Total phosphorus 0.3；SS:10mg/L.
Water quality indicator (after vs EMP is administered before administering)
In the case where hot weather, algae quickly breed, only the improvement time in 2 months, pass through above-mentioned compound EM microorganism formulation
Application method be core ecological management means, comprehensively control water eutrophication, eliminate the algae of pernicious growth, it is transparent
Degree is increased to 60cm by original 15cm, and water quality is promoted to IV class water standard of earth's surface.
River water body can substantially cut down major pollutants after administering, and can quickly solve the problems, such as that river is black smelly.Effluent quality is good
And stablize, effluent quality can match in excellence or beauty with IV water phase, and EMP technique can be effectively dirty from the pollutant, particle removed in water in water
Object and microorganism, heavy metal contaminants etc. are contaminated, such as: COD, BOD, SS, phosphate, coloration, turbidity etc. have very phosphate and SS
More preferably, water quality is promoted to IV class water standard of earth's surface to good removal effect.
To the removal rate of major pollutants (after vs EMP is administered before administering)
After being administered by the application method of compound EM microorganism formulation, water quality data situation of change is as shown in Figure 1, Figure 2 and Figure 3,
Fig. 1 is the COD change curve of the water body after the application method of compound EM microorganism formulation of the invention is administered；Fig. 2 is warp
Cross compound EM microorganism formulation of the invention application method administer after water body ammonia nitrogen change curve；Fig. 3 is by this hair
The total phosphorus change curve of water body after the application method of bright compound EM microorganism formulation is administered.
It can be seen that from curve graph, by ecological management, COD, ammonia nitrogen, total phosphorus testing result have reached earth's surface water ring in river
IV class water quality standard of border quality.Wherein, in governance process there is fluctuation in water quality data, be since algae death releases largely
The pollutants such as nitrogen, phosphorus cause index temporary ascendant trend occur.To sum up, no matter from appearance or Data Detection, Yiyang
Imperial family, city, black and odorous water section, river is significantly improved by sub- brightness ecological, environmental protective EMP ecological control techniques.Microorganism takes it
In naturally, being used for naturally, having restored also to enrich bio-diversity and enhancing river self-cleaning other than the clean looks of river script
Ability reaches the regulation effect of lasting stability.
It is added once according to the 0.05 ‰ of black and odorous water such as lake river processing volume again after adding two weeks for the first time,
It is monthly added later according to 0.05 ‰, black and odorous water mud decrement is stablized in 20%-15-45%.If pressing 10,000 tons of aquatic products of every processing
Raw 5-8 tons of black and odorous water sludge calculate, the daily 200,000 tons of water of black and odorous water, generate 100-160 tons of black and odorous water sludge, disposition takes
It is calculated to 15-450 yuan/ton, daily rate is in 15-45000-48000 member, 10,950,000 yuan of -1,752 ten thousand yuan/year.With black smelly water
The processing of body Sludge Reduction Technologies can reduce remaining black and odorous water sludge 15-45% and calculate, and can save ten thousand yuan/year of 329-526.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of compound EM microorganism formulation, including following strain and mass percent:
Saccharomycete 6%-10%, actinomyces 5%-11%, Bacillus acidi lactici 8%-12%, Plesiomonas 1%-4%, pseudomonas 1%-
5%, enterobacteria belongs to 1%-4%, Bacillus cercus 2%-7%, bacillus subtilis 6%-10%, acinetobacter calcoaceticus 2%-7%, short
Bacillus 1%-4%, Micrococcus 1%-4%, Flavobacterium 1%-5%, Plesiomonas 1%-4%, streptococcus 1%-5%, citric acid
Pseudomonas 1%-5%, Arthrobacter 1%-5%, bacillus 2%-7%, streptococcus 1%-5%, pseudomonas aeruginosa 1%-4%, fluorescence are false single
Born of the same parents bacterium 2%-7%, Nitrobacter 2%-7%, Nitrosococcus 2%-7%, Nitromonas 2%-7%, Alcaligenes 1%-4%.
2. compound EM microorganism formulation according to claim 1, which is characterized in that the compound EM microorganism formulation includes
Following strain and mass percent: saccharomycete 8%, actinomyces 7%, Bacillus acidi lactici 10%, Plesiomonas 2%, pseudomonas
3%, enterobacteria belongs to 2%, Bacillus cercus 5%, bacillus subtilis 8%, acinetobacter calcoaceticus 5%, brevibacterium 2%, microballoon
Pseudomonas 2%, Flavobacterium 3%, Plesiomonas 2%, streptococcus 3%, Klebsiella 3%, Arthrobacter 3%, bacillus 5%,
Streptococcus 3%, pseudomonas aeruginosa 2%, Pseudomonas fluorescens 5%, Nitrobacter 5%, Nitrosococcus 5%, nitrosomonas
Belong to 5%, Alcaligenes 2%.
3. a kind of preparation method of compound EM microorganism formulation, which comprises the steps of: by claims 1 or 2 institute
Each strain in compound EM microorganism formulation stated is inoculated into according to its mass percent equipped with fermentation training after liquid spreads cultivation
In the fermentor for supporting base 1L, inoculum concentration 5%-20%, cultivation temperature is 15-45 DEG C, stirring, when viable count is up to 109When above, obtain
To the compound EM strain preparation.
4. the preparation method of compound EM microorganism formulation according to claim 3, which is characterized in that inoculum concentration 5%, training
Supporting temperature is 15 DEG C.
5. the preparation method of compound EM microorganism formulation according to claim 3, which is characterized in that inoculum concentration 15%, training
Supporting temperature is 20 DEG C.
6. the preparation method of compound EM microorganism formulation according to claim 3, which is characterized in that the fermentation medium
1L includes following component and mass percent: corn flour 1.0%-4.0%, brown sugar 0.3%-0.7%, beancake powder or fish meal 0.3%-
0.7%、CaCO30.3%-0.7%、(NH4)2SO40.08%-0.12%、K2HPO40.01%-0.05%、MgSO4.7H2O0.01%-
0.04% and MnSO4.H2O0.01%-0.04%。
7. the preparation method of compound EM microorganism formulation according to claim 3, which is characterized in that the fermentation medium
1L includes following component and mass percent:
Corn flour 2.0%, brown sugar 0.5%, beancake powder or fish meal 0.5%, CaCO30.5%、(NH4)2SO40.1%、K2HPO40.03%、
MgSO4.7H2O0.02% and MnSO4.H2O0.02%。
8. a kind of application method of compound EM microorganism formulation, which comprises the steps of: added in black and odorous water
Initial concentration is 0.1 ‰ -1.0 ‰ compound EM microorganism system as claimed in claim 1 or 2 of black and odorous water processing volume
Agent.
9. the application method of compound EM microorganism formulation according to claim 8, which is characterized in that thrown in black and odorous water
Adding initial concentration is 0.1 ‰ compound EM microorganism formulation as claimed in claim 1 or 2 of black and odorous water processing volume.
10. the application method of compound EM microorganism formulation according to claim 8, which is characterized in that in black and odorous water
Add the 1.0 ‰ compound EM microorganism formulation as claimed in claim 1 or 2 that initial concentration is black and odorous water processing volume.
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| CN110980961A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Denitrifying microbial inoculum and sewage treatment technology applying same |
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|---|---|---|---|---|
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| CN105645710A (en) * | 2014-11-19 | 2016-06-08 | 辽宁惠源生物环保科技有限公司 | Method for sludge reduction by using compound microbial preparation |
| KR20180075986A (en) * | 2016-12-27 | 2018-07-05 | 민경갑 | A Method for functional fermentation composition |
| CN108358322A (en) * | 2018-02-07 | 2018-08-03 | 山东九生物科技股份有限公司 | A kind of strain formula and preparation method thereof for administering black and odorous water |
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2019
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| CN105645710A (en) * | 2014-11-19 | 2016-06-08 | 辽宁惠源生物环保科技有限公司 | Method for sludge reduction by using compound microbial preparation |
| KR20180075986A (en) * | 2016-12-27 | 2018-07-05 | 민경갑 | A Method for functional fermentation composition |
| CN108358322A (en) * | 2018-02-07 | 2018-08-03 | 山东九生物科技股份有限公司 | A kind of strain formula and preparation method thereof for administering black and odorous water |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110980961A (en) * | 2019-11-26 | 2020-04-10 | 浙江永续环境工程有限公司 | Denitrifying microbial inoculum and sewage treatment technology applying same |
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