CN101054242A - Application of planococcus psychrotoleratus in treating sewage at low temperature - Google Patents

Application of planococcus psychrotoleratus in treating sewage at low temperature Download PDF

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CN101054242A
CN101054242A CN 200710065134 CN200710065134A CN101054242A CN 101054242 A CN101054242 A CN 101054242A CN 200710065134 CN200710065134 CN 200710065134 CN 200710065134 A CN200710065134 A CN 200710065134A CN 101054242 A CN101054242 A CN 101054242A
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planococcus
sewage
cold
psychrotoleratus
resistant
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CN101054242B (en
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刘双江
刘志培
姜嘉彤
陈莉萍
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Institute of Microbiology of CAS
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Institute of Microbiology of CAS
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Abstract

The invention discloses a applications of a strain of Planococcus psychrotoleratus in low temperature sewage disposal, which is the Planococcus psychrotoleratus AS 1.2722 applied in low temperature sewage disposal. In the low temperature sewage disposal method, Planococcus psychrotoleratus AS 1.2722 is inoculated in sewage, cultured in 10 DEG C to 25 DEG C. The invention utilizes the Planococcus psychrotoleratus AS 1.2722 under lower temperature, may efficiently remove BOD, COD, egg white, fat and the starch in sewage, has very high application value in biological processing which low temperature sewage/waste water (such as sewage) containing this kind of contaminant.

Description

The application of one planococcus psychrotoleratus in low-temperature sewage is handled
Technical field
The present invention relates to the application of a planococcus psychrotoleratus in low-temperature sewage is handled, the application of particularly cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 in low-temperature sewage is handled.
Background technology
Along with developing rapidly of China worker, agricultural, problem of environmental pollution becomes in the Chinese society evolution very serious and press for the problem of solution day by day, it has not only restricted the Economic development of China and the raising of living standards of the people, and seriously limited the Sustainable development of Chinese society, people ' s health is produced greatly threat, according to Chinese Academy of Sciences's initial estimate, the current loss that is caused by environmental pollution and ecological damage of China has accounted for 15% of GDP total value, environmental problem has become the demon who engulfs economic gain.
Environment protection has become a major issue in current international relations, the economic cooperation, also day by day seriously affects the Sustainable development of Chinese national economy.In China's Economic development in the past few decades, owing to ignored developing environment protection, environmental aspect is very severe at present.Though taked a large amount of measure of control in recent years, the environmental quality downward trend is still continuing.Discharged waste water is also increasing, and national wastewater emission amount was 45,000,000,000 tons in 2005; The first half of the year in 2006,689.6 ten thousand tons of national chemical oxygen demand (COD) (COD) total emission volumns increase by 3.7% on a year-on-year basis; Sulfurous gas (SO 2) 1274.6 ten thousand tons of total emission volumns, increase by 4.2% on a year-on-year basis." current total emissions of major pollutants reduction task is very arduous.For this reason; each department, all departments will further strengthen the environment protection sense of duty, the sense of urgency; in strict accordance with State Council's requirement; fully implement a scientific outlook on development; actively promote the industry restructuring and the change in the style of economic growth; strengthen the dynamics of Pollution abatement, the realization of Eleventh Five-Year Plan total amount reduction target is guaranteed in the environmental enhancement supervision and management ".The wastewater treatment rate about 70% of present China; the sewage disposal compliance rate is lower; even in the Guangdong Province that sewage disposal is done preferably; it handles compliance rate also only is about 70%; the vast zone of other of China; northern area particularly, about 5-15 ℃, its processing efficiency also will reduce more than 30% the sewage water temperature in winter (during the fingering biological treatment system) than usual.
Sanitary sewage is the sewage from family, office, commerce and urban public utilities and urban runoff.Fresh municipal effluent can oldly gradually descend dissolved oxygen content with corruption, the anaerobic degradation reaction occurs, produces hydrogen sulfide, mercaptan, indoles and skatole, makes the glassware for drinking water foul smelling.The composition of sanitary sewage 99% is a water, and solid impurity mostly is non-toxic substance greatly less than 1%, and wherein inorganic salt have prussiate, vitriol, phosphoric acid salt, ammonium salt, nitrite, nitrate and some heavy carbonic pave floor with bricks, stones etc.s etc.; Organic substance has Mierocrystalline cellulose, starch, carbohydrate, fat, protein and urea etc., also has various washing composition and trace metal in addition, the latter such as zinc, copper, chromium, manganese, nickel and lead etc.; Also contain a large amount of assorted bacterium in the sanitary sewage, be mainly coliform.The content of the phosphorus of nitrogen is mainly derived from commercial sewage, urban ground runoff and ight soil, washing composition etc. than higher in the sanitary sewage in addition.
City domestic sewage is handled and since the Industrial Revolution, more and more had been subject to people's attention before 200 years.Treatment rate of domestic sewage has become an important symbol that whether civilized the area is.Over nearly 200 years, municipal sewage treatment from primary handle naturally, simple one-level handles to develop into and utilizes various modern techniquies, deeply treating wastewater, and reuse.Treatment process also develops into A/O, A from traditional activated sludge process, oxidation ditch process 2/ O, AB, SBR kinds of processes such as (comprising CCAS technology) are to reach different water outlet requirements.The sewage disposal practice of decades shows, Environmental Biotechnology is the treatment technology that has superiority in the wastewater treatment most, it also is present the most frequently used treatment technology, its the most basic advantage is " it is pollutent technology for eliminating rather than separated from contaminants technology " (Biotechnology Newswatch, August 16,1993).Thereby be subjected to the great attention of countries in the world.What deserves to be mentioned is that nearly all city domestic sewage is handled and all adopted with the microbiological treatment technology is the treatment process of core.
In the biologic treating technique of waste water, no matter be the suspension cell method, or activated sludge process or biomembrance process or add the biological einforcement method or the additive method of Black Liquor with Efficient Bacteria, its key core is the microorganism in the treatment system, comprises anaerobic grain sludge and aerobic activated sludge, microbial film and the efficient degrading bacteria that adds.Microorganism in the currently used various biological treatment system all is some normal temperature microorganisms, and the life quintessence of normal temperature microorganism has determined their optimal temperature scopes usually between 20-35 ℃, when temperature is reduced to 15 ℃ or following, its vital movement, comprise growth and breeding and metabolic activity, be subjected to strongly inhibited, also just be difficult to effective degradation of contaminant, so the reduction of processing efficiency is the inevitable outcome of present biologic treating technique under the winter low temperature condition.
Increasingly sharpen discharging standards simultaneously also under the situation of increasingly stringent at environmental situation, pay close attention to and pay attention to, at present also not well solution thinking and way though the problem that sewage treating efficiency is low under the cold condition has caused.In order to improve the efficient of the Sewage treatment systems under the winter low temperature condition, the method that adopts at present there is no additive method except adding gentle insulation measure both at home and abroad; And add the investment that gentle insulation measure has not only increased equipment, and improved operational and administrative expenses, main is to be difficult to carry out in large-scale sewage work.In order to solve the problem that the winter low temperature processing efficiency reduces, basic way is to research and develop the psychrophile bacterial classification that still has efficient degradation pollutent ability under cold condition.
Microbiological current research and development show, a large amount of microorganisms is also surviving on ground such as the low temperature environment such as the South Pole, the arctic, glacier, they still can carry out growth faster under cold condition, psychrophile can be divided into two big classes according to its growth temperature: (1) psychrophilic bacteria (psychrophiles) and psychrotroph (psychrotrophs).Psychrophilic bacteria refers to growth temperature range at-2-20 ℃, the microorganism that optimum growth temperature is about 10 ℃, and this quasi-microorganism still has quick growth and breeding, keeps the ability of high reactivity metaboilic level under 5-15 ℃ of condition; Psychrotroph refers to growth temperature range at 7-30 ℃, the microorganism that optimum growth temperature is about 20 ℃, and this quasi-microorganism still has quick growth and breeding, keeps the ability of high reactivity metaboilic level under 15-25 ℃ of condition.Sewage in the winter/water wasting water temperature of the vast northern area of these microbial growth temperature ranges and China matches, and is that a class can develop, valuable water treatment microorganism resource.
Cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722: Gram-negative, somatic cells are shaft-like.The substratum requirement, the LB substratum: peptone 10g, yeast extract 5g, sodium-chlor 10g is settled to 1000ml after the adding distil water dissolving, the pH nature.This bacterial strain has the 16SrRNA gene order shown in sequence in the sequence table 1.
Summary of the invention
The purpose of this invention is to provide the application of a planococcus psychrotoleratus in low-temperature sewage is handled, the application of promptly cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 in low-temperature sewage is handled.
The present invention also provides and utilizes cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 to carry out the method that low-temperature sewage is handled.
The method that low-temperature sewage provided by the present invention is handled is to utilize cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, carry out sewage disposal under cold condition, concrete grammar is cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 to be inoculated in the sewage cultivate down at 10 ℃ to 25 ℃.
The temperature that described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 cultivates in sewage is preferably 18-22 ℃.
Described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 inoculum densities are not less than 10 5, in order to save the cost input, the scope of described inoculum density can be 10 5-10 8Cfu/mL is preferably 10 7Cfu/mL.
Described sewage can be sanitary sewage, starch factory sewage, holds fowl aquaculture wastewater or slaughterhouse sewage, is preferably sanitary sewage.
The 16S rRNA gene of described cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 has the nucleotide sequence shown in sequence in the sequence table 1.
The present invention utilizes the characteristics of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 tolerance low-temperature epitaxy environment, using it for low-temperature sewage handles, experimental results show that, cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 is inserted in the sanitary sewage, (10-25 degree centigrade) this strain growth is good under lower temperature, can effectively remove starch in the sewage, albumen, fat and BOD, COD, in the biological treatment that contains the low-temperature sewage/waste water of this pollutant (as sanitary sewage), has very high using value.
Description of drawings
Fig. 1 is the growth curve of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722
Embodiment
Method among the following embodiment if no special instructions, is ordinary method.
Percentage composition among the following embodiment if no special instructions, is the quality percentage composition.
The cultivation of embodiment 1, cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 and growth curve are measured
With aseptic technique corresponding bacterial classification is received in the substratum, cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 is pressed 10 4The amount of cfu/mL is inoculated in 200ml LB substratum (peptone 10g is housed, yeast extract 5g, sodium-chlor 10g, be settled to 1000ml after the adding distil water dissolving, 121 ℃, 30min steam sterilizing) the Erlenmeyer flask of 500ml in, after the inoculation, controlled temperature is 12 ℃ on the low temperature shaking table, rotating speed 150rpm, and the turbidity (OD of culture is measured in certain hour sampling (5mL) at interval 600), draw growth curve.The result as shown in Figure 1, the result shows, under 12 ℃, about 40 hours of the delayed growth phase of southern cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, needs about 80 hours cultivation can reach maximum growth amount, OD 600Can reach (Fig. 1) more than 2.0.
The degraded enzyme of embodiment 2, cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722
Is the characteristics of principal pollutant according to sanitary sewage with starch, albumen and fat, qualitative test the relevant enzymes of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722.Diastatic mensuration adopts starch flat board---IKI method, if bacterial strain can form the transparent circle greater than bacterium colony on the starch flat board, contains amylase at this bacterial strain of explanation; The mensuration of proteolytic enzyme adopts the casein hydrolysis method, if bacterial strain can caseinhydrolysate discharged amino acid then illustrate that this bacterial strain has protease activities; The mensuration of lipase adopts dull and stereotyped---and the tween method if bacterial strain can form transparent circle on the tween flat board, illustrates that then this bacterial strain contains lipase.The result shows that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 has proteolytic enzyme, amylase and lipase, and the existence of these enzymes helps bacterial strain is brought into play its degradation of contaminant in treatment system function and effect.
The degraded of embodiment 3,1.2722 pairs of starch of cold-resistant planococcus (Planococcus psychrotoleratus) AS
Preparation starch culture-medium: Na 2HPO 4, 2.0g; KH 2PO 4, 1.0g; MgSO 47H 2O, 0.1g; (NH 4) 2SO 4, 0.5g; NaCl, 10g; Zulkovsky starch, 6.8g; Inorganic salt solution, 5mL; H 2O, 1000mL; PH 7.2.Described inorganic salt solution is: EDTA 0.5g, ZnSO 47H 2O 0.22g, CaCl 20.055g, MnCl 24H 2O0.051g, FeSO 47H 2O 0.0499g, (NH 4) 6Mo 7O 244H 2O 0.011g, CuSO 45H 2O 0.0157g, CoCl 26H 2O 0.0161g, water is settled to 1000mL, and pH 6.0.Substratum is sub-packed in 100mL in the triangular flask of 250mL, 121 ℃ of sterilization 30min.
The cooling back presses 10 with a small amount of cold-resistant planococcus of transfering loop picking (Planococcus psychrotoleratus) AS1.2722 4The amount of cfu/mL is inoculated in the above-mentioned starch culture-medium, cultivates (150rpm) with 12 ℃ of shaking tables, and irregularly strain growth amount and starch residual content are measured in sampling (5mL).The mensuration of strain growth is represented with the turbidity at wavelength 600nm place; The mensuration of starch adopts IKI colour developing spectra photometric method.The result who cultivates 10 days is as shown in table 1.The result shows that 1.2722 pairs of starch of cold-resistant planococcus (Planococcus psychrotoleratus) AS all have good degradation capability, reach more than 66% through the degradation rate of 10 days cultivations to starch, can be used for the processing of sewage starch fully.
The degraded of 1.2722 pairs of starch of the cold-resistant planococcus of table 1. (Planococcus psychrotoleratus) AS
Bacterial strain Strain growth (OD 600) Starch residual content (g/L) Starch clearance (%)
Cold-resistant planococcus (Planococcus psychrotolera tus) AS 1.2722 ??0.57 ??2.3 ??66.2
The treatment effect experiment of embodiment 4,1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcus psychrotoleratus) AS
Is principal pollutant according to sanitary sewage with starch, albumen and fat, and its COD content is about the characteristics that 300mg/L and C: N: P is about 100: 5: 1, the preparation simulated domestic wastewater: with glucose+starch (each 50%), NH 4Cl+ peptone (each 50%), K 2HPO 4And the inorganic salt solution of 5mL/L is mixed with the simulated domestic wastewater that COD is 300mg/L (glucose 0.15g/L, starch 0.15g/L, yeast powder 0.01g/L, NH 4Cl 0.01g/L, KH 2PO 40.005g/L, inorganic salt solution 5mL/L).Wherein, inorganic salt solution is with the inorganic salt solution of embodiment 3.This simulated domestic wastewater COD is 300mg/L, and BOD content is that 285mg/L, starch content are 150mg/L, and protein content is 10mg/L.
To the simulation test that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 carries out sanitary sewage disposal, concrete steps are as described below:
Cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 with a little slant culture of inoculating needle picking is inoculated in the 250mL triangular flask that contains the above-mentioned simulated domestic wastewater of 100mL, 12 ℃ of shaking tables are cultivated, and irregularly the growing state of bacterial strain in simulated domestic wastewater and the removal effect of COD are measured in sampling (5mL).The mensuration of strain growth is represented with the turbidity at wavelength 600nm place; The mensuration of COD adopts the mensuration of National Standard Method (GB11914-89), starch to adopt IKI method, proteic mensuration to adopt Bradford method, BOD 5Mensuration adopt 5 days standard laws to measure every index before and after cultivating.Cultivate shown in the detected result table 2 after 3 days, the result shows, under 12 ℃ condition, cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 can adapt to simulated domestic wastewater better, and consider that this is only to cultivate the result who obtained in 3 days under the very little condition of inoculum size, if the reality of being applied to, under the situation of big inoculum size, processing power will be further enhanced.
The treatment effect of 1.2722 pairs of simulated domestic wastewaters of the cold-resistant planococcus of table 2. (Planococcus psychrotoleratus) AS.
Index Residual content (mg/L) Clearance (%)
Strain growth (OD 600) ??COD ??BOD 5Starch albumen ??0.017 ??217 ??206 ??130 ??8.5 ??- ??27.7 ??29 ??13.3 ??15
The treatment effect experiment of embodiment 5, big 1.2722 pairs of simulated domestic wastewaters of the cold-resistant planococcus of inoculum size (Planococcus psychrotoleratus) AS
According to prescription described in the embodiment 4 and composition preparation simulated domestic wastewater, with 1.5 * 10 8The inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 to this simulated domestic wastewater, according to embodiment 4 described culture condition, shaking table was cultivated after 3 days, adopted the measuring method as embodiment 4, had measured COD, BOD before and after cultivating 5, starch, protein content, the result is as shown in table 4, under the situation of big inoculum size, 1.2722 pairs of simulated domestic wastewaters of cold-resistant planococcus (Planococcus psychrotoleratus) AS have the good treatment effect, and the clearance of every pollutent index can reach (except that albumen) more than 90%.
The treatment effect of 1.2722 pairs of simulated domestic wastewaters of the cold-resistant planococcus of table 4. (Planococcus psychrotoleratus) AS
Index Residual content (mg/L) Clearance (%)
Strain growth (OD 600) ??COD ??BOD 5Starch albumen ??0.121 ??13 ??9 ??15 ??1.5 ??- ??95.7 ??97 ??90 ??85
The processing experiment of drawing together sewage is given birth in 1.2722 pairs of simulations of cold-resistant planococcus under embodiment 6, the differing temps (Planococcus psychrotoleratus) AS
According to prescription described in the embodiment 4 and composition preparation simulated domestic wastewater, with 10 6The inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722, the increment of bacterial strain and COD, BOD, starch and the proteic changing conditions of simulated domestic wastewater are measured in the processing of 1.2722 pairs of simulated domestic wastewaters of test cold-resistant planococcus (Planococcus psychrotoleratus) AS under different temperature after cultivating 3 days.The result is as shown in table 4, and the result shows, cold-resistant planococcus (Planococcuspsychrotoleratus) but AS 1.2722 at 8-25 ℃ of equal treatment of simulated sanitary sewage, preferred range is 12-18 ℃.
The processing of 1.2722 pairs of simulated domestic wastewaters of (Planococcus psychrotoleratus) AS of cold-resistant planococcus under table 4. differing temps
Temperature (℃) Index Residual content (mg/L) Clearance (%)
??8 Growth (OD 600) ?COD ?BOD 5Starch albumen ??0.01 ??252 ??240 ??141 ??9.5 ??- ??16 ??17 ??6 ??5
??12 Growth (OD 600) ?COD ?BOD 5Starch albumen ??0.017 ??217 ??206 ??130 ??8.5 ??- ??27.7 ??29 ??13.3 ??15
??18 Growth (OD 600) ?COD ?BOD 5Starch albumen ??0.019 ??210 ??201 ??128 ??8.3 ??- ??30 ??30.7 ??14.7 ??17
??25 Growth (OD 600) ?COD ?BOD 5Starch albumen ??0.015 ??225 ??216 ??135 ??9 ??- ??25 ??25.5 ??10 ??10
The cold-resistant planococcus of embodiment 7 different vaccination amounts (Planococcus psychrotoleratus) AS1.2722 is to the processing experiment of simulated domestic wastewater
According to prescription described in the embodiment 4 and composition preparation simulated domestic wastewater, with 10 5Cfu/mL, 10 6Cfu/mL, 10 7Cfu/mL and 10 8The inoculum size of cfu/mL is inoculated cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722, the processing of 1.2722 pairs of simulated domestic wastewaters of test cold-resistant planococcus (Planococcuspsychrotoleratus) AS under 12 ℃ of temperature, measure the increment of bacterial strain and COD, BOD, starch and the proteic changing conditions of simulated domestic wastewater after cultivating 3 days, the result is as shown in table 6.Show that it is 10 that cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 selects the inoculum size scope 7-10 8
The processing of 1.2722 pairs of simulated domestic wastewaters of (Planococcus psychrotoleratus) AS of cold-resistant planococcus under the table 6 different vaccination amount
Inoculum size Index Residual content (mg/L) Clearance (%)
??10 5cfu/mL Growth (OD 600) ??COD ??BOD 5Starch albumen ??0.10 ??65 ??55 ??35 ??4.5 ??- ??78.3 ??81 ??76.7 ??55
??10 6cfu/mL Growth (OD 600) ??COD ??BOD 5Starch albumen ??0.113 ??18 ??12 ??16 ??1.5 ??- ??94 ??96 ??89.3 ??85
??10 7cfu/mL Growth (OD 600) ??COD ??BOD 5Starch albumen ??0.121 ??13 ??9 ??15 ??1.5 ??- ??95.7 ??97 ??90 ??85
??10 8cfu/mL Growth (OD 600) ??COD ??BOD 5Starch albumen ??0.123 ??12 ??9 ??12 ??1.5 ??- ??96 ??97 ??92 ??85
Sequence table
<160>1
<210>1
<211>1517
<212>DNA
<213〉cold-resistant planococcus (Planococcus psychrotoleratus)
<400>1
ccccgctcag?gacgaacgct?ggcggcatgc?ctaatacatg?caagtcgagc?ggaaccagga????60
gagcttgctc?tttctggttc?agcggcggac?gggtgagtaa?cacgtgggca?acctgccctg????120
cagatcggga?taactccggg?aaaccggtgc?taataccgaa?tagtttggag?cctctcctga????180
ggctccacgg?aaaggcggct?tcggctgcca?ctgcaggatg?ggcccgcggc?gcattagcta????240
gttggtgggg?taatggccta?ccaaggcgac?gatgcgtagc?cgacctgaga?gggtgatcgg????300
ccacactggg?actgagacac?ggcccagact?cctacgggag?gcagcagtag?ggaatcttcc????360
gcaatggacg?caagtctgac?ggagcaatgc?cgcgtgagtg?acgaaggttt?tcggatcgta????420
aaactctgtt?gtaagggaag?aacaagcccc?atttaactga?tggggccctg?acggtacctt????480
accagaaagc?cacggctaac?tacgtgccag?cagccgcggt?aatacgtagg?tggcaagcgt????540
tgtccggaat?tattgggcgt?aaagcgcgcg?caggcggtcc?cttaagtctg?atgtgaaagc????600
ccacggctca?accgtggagg?gtcattggaa?actgggggac?ttgagtgcag?aagaggaaag????660
tggaattcca?tgtgtagcgg?tgaaatgcgt?agagatgtgg?aggaacacca?gtggcgaagg????720
cgactttctg?gtctgtaact?gacgctgagg?cgcgaaagcg?tggggagcaa?acaggattag????780
ataccctggt?agtccacgcc?gtaaacgatg?agtgctaagt?gttagggggt?ttccgcccct????840
tagtgctgca?gctaacgcat?taagcactcc?gcctggggag?tacggccgca?aggctgaaac????900
tcaaaggaat?tgacgggggc?ccgcacaagc?ggtggagcat?gtggtttaat?tcgaagcaac????960
gcgaagaacc?ttaccaggtc?ttgacatccc?gctgcccggc?gtagagatac?gcttttccct????1020
tcggggacag?cggtgacagg?tggtgcatgg?ttgtcgtcag?ctcgtgtcgt?gagatgttgg????1080
gttaagtccc?gcaacgagcg?caacccttga?tcttagttgc?cagcattcag?ttgggcactc????1140
taaggtgact?gccggtgaca?aaccggagga?aggtggggat?gacgtcaaat?catcatgccc????1200
cttatgacct?gggctacaca?cgtgctacaa?tggacggtac?aaagggctgc?caacccgcga????1260
gggggagcca?atcccagaaa?accgttctca?gttcggattg?caggctgcaa?ctcgcctgca????1320
tgaagccgna?atcgctagta?atcgtggatc?agcatgccac?ggtgaatacg?ttcccgggcc????1380
ttgtacacac?cgcccgtcac?accacgagag?tttgtaacac?ccgaagtcgg?tgaggtaacc????1440
ccttgtggga?gccagccgcc?gaaggtggga?cagatgattg?gggtgaagtc?gtaacaaggt????1500
agccgtatcg?gaaggtg???????????????????????????????????????????????????1517

Claims (7)

1, the application of cold-resistant planococcus (Planococcus psychrotoleratus) AS 1.2722 in low-temperature sewage is handled.
2, a kind of method of low-temperature sewage processing is cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 to be inoculated in the sewage cultivate down at 10 ℃ to 25 ℃.
3, method according to claim 2 is characterized in that: described cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 optimum temperature range of disposing of sewage is 12-18 ℃.
4, method according to claim 3 is characterized in that: described cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 inoculum densities are not less than 10 5
5, method according to claim 4 is characterized in that: described cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 inoculum densities are 10 5-10 8Cfu/mL is preferably 10 7-10 8Cfu/mL.
6, method according to claim 5 is characterized in that: described sewage is sanitary sewage.
7, method according to claim 6 is characterized in that: the 16S rRNA gene of described cold-resistant planococcus (Planococcuspsychrotoleratus) AS 1.2722 has the nucleotide sequence shown in sequence in the sequence table 1.
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CN108611291A (en) * 2018-04-08 2018-10-02 哈尔滨工业大学(威海) One plant of salt tolerance planococcus and its application
CN109019875A (en) * 2018-09-10 2018-12-18 余素萍 A kind of method of phosphorus and nitrogen removal effect in raising low-temperature sewage
CN109133518A (en) * 2018-09-19 2019-01-04 余素萍 A kind of improved method for improving sanitary sewage disposal efficiency under cryogenic conditions

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CN1384065A (en) * 2001-04-28 2002-12-11 沈阳碧水蓝天环境科技有限公司 Biological treating process of high-concentration organic waste water
CN1215991C (en) * 2002-12-24 2005-08-24 中国科学院微生物研究所 Low temperature nitrobacter agent and use thereof

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CN108611291A (en) * 2018-04-08 2018-10-02 哈尔滨工业大学(威海) One plant of salt tolerance planococcus and its application
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