CN109820003A - A kind of terrestrial plant source algae-inhibiting agent and preparation method thereof - Google Patents
A kind of terrestrial plant source algae-inhibiting agent and preparation method thereof Download PDFInfo
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- CN109820003A CN109820003A CN201910139621.0A CN201910139621A CN109820003A CN 109820003 A CN109820003 A CN 109820003A CN 201910139621 A CN201910139621 A CN 201910139621A CN 109820003 A CN109820003 A CN 109820003A
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- algae
- aloe
- inhibiting agent
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Abstract
The invention discloses a kind of terrestrial plant source algae-inhibiting agent and preparation method thereof, the algae-inhibiting agent is aloe leaching liquor, and weight concentration of the aloe leaching liquor in water body is not less than 4%.The present invention is compared with prior art, algae-inhibiting agent is prepared using terrestrial plant extracting solution, made algae-inhibiting agent is in addition to 1% processing group of low concentration, each processing group Microcystis aeruginosa Strains are in reduction trend, with the increase of concentration, inhibiting rate is also obviously reinforced, and especially 8% processing group of high concentration has reached 90% in inhibiting rate of the third day to Microcystis aeruginosa Strains.
Description
Technical field
The invention belongs to algae-inhibiting agents and preparation method thereof, particularly belong to terrestrial plant source algae-inhibiting agent and preparation method thereof.
Background technique
Rural area in south China, blue-green alge are widely present in the water bodys such as pond, streamside, and microcystin can be generated by having now been found that
The Trentepohlia of plain (Microcystin) has Microcystis aeruginosa, anabena and the algae that quivers, and most of wawter blooms of China's discovery are all Microcystis aeruginosas.
The Microcystin that Microcystis aeruginosa generates is very harmful, and exploration discovery is a kind of efficient, and the small algal control method of ecological risk is current
The break-through point of improvement.
Summary of the invention
A kind of technical problems to be solved by the invention the 1st good, the terrestrial for Microcystis aeruginosa that is to provide ecological security
Plant source algae-inhibiting agent.
The technical problems to be solved by the invention the 2nd be above-mentioned terrestrial plant source algae-inhibiting agent preparation method.
The technical solution of present invention solution technical problem are as follows: a kind of terrestrial plant source algae-inhibiting agent, the algae-inhibiting agent are reed
Luxuriant growth leaching liquor, weight concentration of the aloe leaching liquor in water body are not less than 4%.
The aloe leaching liquor, is prepared by the following method:
It is blotted biennial fresh aloe (Aloe chinensisBaker) whole strain distilled water repeated flushing and with filter paper
Its droplet takes leaf to shred and is put into mortar after weighing, quartzite sand grind to less than 200 mesh are added, and filtrate, distilled water are collected in filtering
Filter residue is cleaned, filtrate and cleaning solution collect to volumetric flask, is settled to that scale is spare, and 0.8-1.2 grams of aloe constant volume holds in 10 milliliters
Measuring bottle.
The algae is microcystic aeruginosa.
The algae density is 1-9*105A/milliliter.
Control for cyanobacteria, the allelopathic alga-inhibiting action of plant is due to the secondary metabolites that its algal control ingredient is plant, energy
It is enough to accumulate in water body natural degradation and cause secondary pollution, there is relatively good ecological security, related research at present compared with
It is more.But the allelopathic alga-inhibiting action of terrestrial plant is also less relative to water plant overall study, due to terrestrial plant and aquatic plant
Object also receives people compared to having the characteristics that sampling is convenient, biomass is big, its allelochemical type is more and rich content at present
Pay close attention to.
Aloe (Aloe chinensis Baker) is Eremurus chinensis section many years terrestrial herbaceous plant, and aloe has more complicated
Chemical component, wherein most importantly barbaloin, also known as aloin (Aloin), aloin etc., are that antibiotic property is very strong
Substance, can kill fungi, mould, bacterium, virus etc., inhibit and the growth and breeding of eliminating pathogen.The biology function of aloe
It can also obtain more widely studying, such as aloe can be used for antibacterial anti-inflammatory, removing toxic substances, healing of wound, but for its allelopathic algal control
There are no any reports for effect.
Compared with prior art, the present invention preparing algae-inhibiting agent using terrestrial plant extracting solution, made algae-inhibiting agent is except low
Outside 1% processing group of concentration, each processing group Microcystis aeruginosa Strains are in reduction trend, and with the increase of concentration, inhibiting rate is also obvious
Reinforce, especially 8% processing group of high concentration has reached 90% in inhibiting rate of the third day to Microcystis aeruginosa Strains.
Detailed description of the invention
Influence of Fig. 1 various concentration aloe leaching liquor to Growth of Microcystis aeruginosa.
Inhibiting rate (%) of Fig. 2 various concentration aloe leaching liquor to microcystic aeruginosa.
Fig. 3 various concentration aloe leaching liquor is on the active influence of microcystic aeruginosa CAT.
Fig. 4 various concentration aloe leaching liquor is on the active influence of microcystic aeruginosa POD.
Fig. 5 various concentration aloe leaching liquor is on the active influence of microcystic aeruginosa SOD.
Specific embodiment
Below with reference to embodiment, the present invention is described in detail.
Algae microcystic aeruginosa (FACHB-942) used is purchased from the research of Chinese Academy of Sciences Wuhan aquatile in the present invention
Institute uses BG-11 culture medium culture.Condition of culture: 25 DEG C of temperature, intensity of illumination 4000lx, light dark period 12h:12h, expand
Big culture one week, makes microcystic aeruginosa reach logarithmic growth phase, and being diluted to initial algae density with BG-11 culture medium is 5 × 105
A/mL.
Aloe is provided by department of horticulture, Anhui Normal University.
The calculation formula of microcystic aeruginosa inhibiting rate are as follows:
Wherein: IR-inhibiting rate, N-experimental group frustule number, N0-control group frustule number.
Method of the active measurement of catalase (CAT) referring to Patra；
Peroxidase (POD) determination of activity is referring to improved Cakmak method；
Method of the active measurement of superoxide dismutase (SOD) referring to the Stewert and Bewley of modification.
Embodiment 1:
It is blotted 2 years raw fresh aloe (Aloe chinensisBaker) whole strain distilled water repeated flushing and with filter paper
Its droplet takes leaf to shred and is put into mortar after weighing, quartzite sand grind to less than 200 mesh are added, and filtrate, distilled water are collected in filtering
Filter residue is cleaned, filtrate and cleaning solution collect to volumetric flask, is settled to that scale is spare, and 1 gram of aloe constant volume is in 10 milliliters of volumetric flasks.
Embodiment 2:
It is blotted 2 years raw fresh aloe (Aloe chinensisBaker) whole strain distilled water repeated flushing and with filter paper
Its droplet takes leaf to shred and is put into mortar after weighing, quartzite sand grind to less than 200 mesh are added, and filtrate, distilled water are collected in filtering
Filter residue is cleaned, filtrate and cleaning solution collect to volumetric flask, is settled to that scale is spare, and 0.8 gram of aloe constant volume is in 10 milliliters of volumetric flasks.
Embodiment 3:
It is blotted 2 years raw fresh aloe (Aloe chinensisBaker) whole strain distilled water repeated flushing and with filter paper
Its droplet takes leaf to shred and is put into mortar after weighing, quartzite sand grind to less than 200 mesh are added, and filtrate, distilled water are collected in filtering
Filter residue is cleaned, filtrate and cleaning solution collect to volumetric flask, is settled to that scale is spare, and 1.2 grams of aloe constant volumes are in 10 milliliters of volumetric flasks.
Embodiment 4
The microcystic aeruginosa of logarithmic growth phase is respectively put into sterilized 100mL conical flask, BG-11 culture is added
Base makes its frustule ultimate density 5.0 × 105Cells/mL, and make final volume 50mL.Embodiment 1 is made in conical flask
The ultimate density of aloe leaching liquor be respectively 1%, 2%, 4%, 8%, while setting the control group that aloe leaching liquor is not added, every group
If 3 are parallel, 24 after sample-adding, 48,72,96,108,120h sampling with blood counting chamber carry out frustule counting.Measurement
Catalase (CAT) activity, peroxidase (POD) activity, superoxide dismutase (SOD) activity.
The condition of culture of each group are as follows: intensity of illumination 4000lx, Light To Dark Ratio 12h:12h, temperature (25 ± 1) DEG C.It shakes daily
It is 3-5 times dynamic.
Its result such as Fig. 1 is shown: with concurrent control group ratio, each concentration aloe leaching liquor can inhibit microcystic aeruginosa just
It is frequently grown, and as the increase inhibiting effect of concentration is more obvious, but is begun to exceed in the algae density that 4d concentration is 1% processing group
Control group illustrates aloe leaching liquor algal control in the high suppression of low rush.
Shown in Fig. 2: at the 1st and the 4th day with the extension of time, inhibiting rate be in increase tendency, and show concentration and when
Between effect.The aloe leaching liquor that concentration is 8% has reached 90% or more in 3d inhibiting rate, shows that aloe leaching liquor is micro- to verdigris
The inhibitory effect of capsule algae is significant；It is negative value that the aloe leaching liquor 4d that concentration is 1%, which starts inhibiting rate, is illustrated in facilitation,
Further demonstrating aloe leaching liquor is the high suppression of low rush to microcystic aeruginosa.
Shown in Fig. 3: in addition to 1% processing group of low concentration, compared with concurrent control group, with the variation of time, the CAT of each group
Downward trend is presented in activity, illustrates that frustule receives serious damage, albumen under the action of high concentration aloe leaching liquor
Matter synthesis is affected, and then enzymatic activity declines.The CAT enzymatic activity of 1% processing group is more than control group in 6d, illustrates that aloe presses down
Algae has the high suppression effect of low rush.For control group, the reason of later period CAT activity declines, declines with frustule aging and metabolic rate
It is related.
Shown in Fig. 4: with the variation of time, each processing group POD activity declines, and illustrates that aloe leaching liquor is micro- to verdigris
The depression effect of capsule algae is significant.1% processing group POD enzymatic activity of low concentration begins to exceed control group from 2d, this and CAT in Fig. 3
Activity change is consistent, again illustrates the low rush height suppression of aloe leaching liquor algal control.
Shown in Fig. 5: with the variation of time, each processing group SOD enzyme activity is on a declining curve, wherein at high concentration 8%
Reason group downward trend becomes apparent, and illustrates that high concentration aloe leaching liquor can effectively inhibit microcystic aeruginosa enzymatic activity.
Claims (4)
1. a kind of terrestrial plant source algae-inhibiting agent, it is characterised in that: the algae-inhibiting agent is aloe leaching liquor, and aloe leaching liquor is in water
Weight concentration in body is not less than 4%.
2. a kind of terrestrial plant source algae-inhibiting agent according to claim 1, it is characterised in that:
The algae is microcystic aeruginosa.
3. a kind of terrestrial plant source algae-inhibiting agent according to claim 2, it is characterised in that: the algae density is 1-9*105
A/milliliter.
4. a kind of terrestrial plant source algae-inhibiting agent described in claim 1, is prepared by the following method:
By biennial fresh aloe (Aloe chinensisBaker) whole strain distilled water repeated flushing and its water is blotted with filter paper
Pearl takes leaf to shred and is put into mortar after weighing, quartzite sand grind to less than 200 mesh are added, and filtrate, distilled water cleaning are collected in filtering
Filter residue, filtrate and cleaning solution are collected to volumetric flask, are settled to that scale is spare, and 0.8-1.2 grams of aloe constant volume is in 10 milliliters of volumetric flasks.
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| CN201910139621.0A CN109820003A (en) | 2019-02-19 | 2019-02-19 | A kind of terrestrial plant source algae-inhibiting agent and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544640A (en) * | 2020-12-15 | 2021-03-26 | 安徽师范大学 | Algae inhibitor derived from sugarcane peel and preparation method thereof |
Citations (2)
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| KR20090115453A (en) * | 2008-05-02 | 2009-11-05 | 한국화학연구원 | Composition comprising menadione based compound for enhansing control of harmful algae, and method for controlling harmful algae using the same |
| CN103704333A (en) * | 2013-12-12 | 2014-04-09 | 海南大学 | Method for freshness keeping of mangos by using aloe extract liquid |
-
2019
- 2019-02-19 CN CN201910139621.0A patent/CN109820003A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090115453A (en) * | 2008-05-02 | 2009-11-05 | 한국화학연구원 | Composition comprising menadione based compound for enhansing control of harmful algae, and method for controlling harmful algae using the same |
| CN103704333A (en) * | 2013-12-12 | 2014-04-09 | 海南大学 | Method for freshness keeping of mangos by using aloe extract liquid |
Non-Patent Citations (3)
| Title |
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| YUSUKE HIRAI 等: ""Effect of growth control to Microcystis aeruginosa by using Aloe barbadensis"", 《日本水环境学会年会讲演集》 * |
| 何海霞 等: ""HPLC法测定芦荟及芦荟饮料中的低分子量有机酸"", 《食品科技》 * |
| 张美娇 等: ""薰衣草精油和芦荟对痤疮面部主要菌群的影响"", 《中国微生态学杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112544640A (en) * | 2020-12-15 | 2021-03-26 | 安徽师范大学 | Algae inhibitor derived from sugarcane peel and preparation method thereof |
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