CN104604950B - Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application - Google Patents
Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application Download PDFInfo
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- CN104604950B CN104604950B CN201510007221.6A CN201510007221A CN104604950B CN 104604950 B CN104604950 B CN 104604950B CN 201510007221 A CN201510007221 A CN 201510007221A CN 104604950 B CN104604950 B CN 104604950B
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- algae
- momordica grosvenori
- inhibiting agent
- leaching liquor
- momordica
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 85
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 70
- 241001409321 Siraitia grosvenorii Species 0.000 title claims abstract description 69
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 title claims abstract description 68
- 238000002386 leaching Methods 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 59
- 235000009815 Momordica Nutrition 0.000 claims abstract description 27
- 241000218984 Momordica Species 0.000 claims abstract description 27
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 14
- 230000012010 growth Effects 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 4
- 238000010348 incorporation Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 12
- 230000000694 effects Effects 0.000 description 8
- 230000001629 suppression Effects 0.000 description 6
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 2
- 235000017048 Garcinia mangostana Nutrition 0.000 description 2
- 240000006053 Garcinia mangostana Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 239000005422 algal bloom Substances 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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Abstract
The invention discloses a kind of Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application, the preparation method includes：A, Momordica grosvenori is crushed and Grosvenor Momordica Fruit is made；B, with obtained Momordica grosvenori suspension in Grosvenor Momordica Fruit, alcohol and water being mixed；C, the Momordica grosvenori suspension is concentrated and Momordica grosvenori leaching liquor algae-inhibiting agent is made.The growth of algae can effectively be suppressed by Momordica grosvenori leaching liquor algae-inhibiting agent made from this method.
Description
Technical field
The present invention relates to algae-inhibiting agent, in particular it relates to a kind of Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application.
Background technology
Alga eruption is a kind of Disasters for frequently occurring on seawater and freshwater waters at present, referred to as red in ocean
Tide, wawter bloom is referred to as in fresh water.The algae that wawter bloom can be formed is mainly the species in Cyanophyta and Chlorophyta.In eutrophication
It is most commonly seen with the microcystic aeruginosa of Cyanophyta and anabena in poisons in freshwater.
Prevent algal bloom and control wawter bloom from turning into the task of top priority.Algae-inhibiting agent is because energy fast onset alga-inhibiting action is so that water
China is eased, so as to one of method as wawter bloom emergency processing.Algae-inhibiting agent can be divided into physical algae-inhibiting agent, chemically press down
Algae agent and biochemical algae-inhibiting agent, the property of these algae-inhibiting agents are as follows：1) physical algae-inhibiting agent：Effect of algae restraint is notable, still
Need the long period to take effect, and the metal ions such as iron, aluminium can be introduced into water body, though pollution is low but still have pollution；2)
Chemically algae-inhibiting agent：It is quick, equally there is toxicity to aquatile while algae is killed, the hidden of secondary pollution often be present
Suffer from；3) biological algae-inhibiting agent：Low toxicity, low cost, therefore as the research direction of current water environment treatment technology.
Or existing biological algae-inhibiting agent be inhibiting rate below 50%, or be algae-inhibiting agent dosage it is larger.
The content of the invention
It is an object of the invention to provide a kind of Momordica grosvenori leaching liquor algae-inhibiting agent and preparation method thereof, by made from this method
Momordica grosvenori leaching liquor algae-inhibiting agent can effectively suppress the growth of algae.
To achieve these goals, the invention provides a kind of preparation method of Momordica grosvenori leaching liquor algae-inhibiting agent, preparation side
Method includes：
A, Momordica grosvenori is crushed and Grosvenor Momordica Fruit is made；
B, with obtained Momordica grosvenori suspension in Grosvenor Momordica Fruit, alcohol and water being mixed；
C, the Momordica grosvenori suspension is concentrated and Momordica grosvenori leaching liquor algae-inhibiting agent is made.
Preferably, the particle diameter of Grosvenor Momordica Fruit is not less than 150 mesh.
Preferably, in stepb, relative to the Grosvenor Momordica Fruit of 1 parts by weight, the dosage of the water is 15-25 parts by weight.
Preferably, in stepb, mixing meets following condition：Incorporation time is 2-4 days, and mixing temperature is 15-35 DEG C.
Preferably, in step c, on the basis of the gross weight of the Momordica grosvenori leaching liquor algae-inhibiting agent, the mangosteen powder
The content at end is 8-12 weight %.
Present invention provides a kind of Momordica grosvenori leaching liquor algae-inhibiting agent, Momordica grosvenori leaching liquor algae-inhibiting agent passes through above-mentioned method
It is prepared.
Present invention also offers a kind of answering in the growth for suppressing algae according to above-mentioned Momordica grosvenori leaching liquor algae-inhibiting agent
With.
Pass through above-mentioned technical proposal, after the present invention is by the way that Momordica grosvenori is crushed, Grosvenor Momordica Fruit and water are then mixed into leaching
Bubble, finally concentrate and Momordica grosvenori leaching liquor algae-inhibiting agent is just made.The algae-inhibiting agent is prepared environmental pollution using pure natural substance
It is small, while the algae-inhibiting agent is high to the inhibiting rate of algae and dosage is small.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
Accompanying drawing is for providing a further understanding of the present invention, and a part for constitution instruction, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the statistical chart for detecting the algae cell density in each part microcystic aeruginosa liquid in example 1；
Fig. 2 is the result statistical chart for detecting inhibiting rate-incubation time in example 1；
Fig. 3 is the result statistical chart for detecting inhibiting rate-incubation time in example 3；
Fig. 4 is the statistical chart for detecting the algae cell density in each part microcystic aeruginosa liquid in example 4.
Embodiment
The embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The invention provides a kind of preparation method of Momordica grosvenori leaching liquor algae-inhibiting agent, preparation method includes：
A, Momordica grosvenori is crushed and Grosvenor Momordica Fruit is made；
B, with obtained Momordica grosvenori suspension in Grosvenor Momordica Fruit, alcohol and water being mixed；
C, the Momordica grosvenori suspension is concentrated and Momordica grosvenori leaching liquor algae-inhibiting agent is made.
In preparation method provided by the invention, the particle diameter of Grosvenor Momordica Fruit can select in wide scope, but be
Cause obtained Momordica grosvenori leaching liquor algae-inhibiting agent that there is the effect of more excellent suppression algal grown, it is preferable that mangosteen powder
The particle diameter at end is not less than 150 mesh.
In preparation method provided by the invention, the dosage of Grosvenor Momordica Fruit and water can select in wide scope, but
It is in order that obtaining obtained Momordica grosvenori leaching liquor algae-inhibiting agent has the effect of more excellent suppression algal grown, it is preferable that in step
In rapid b, relative to the Grosvenor Momordica Fruit of 1 parts by weight, the dosage of the water is 15-25 parts by weight.
In preparation method provided by the invention, the actual conditions of mixing can select in wide scope, but in order to
So that obtained Momordica grosvenori leaching liquor algae-inhibiting agent has the effect of more excellent suppression algal grown, it is preferable that in stepb,
Mixing meets following condition：Incorporation time is 2-4 days, and mixing temperature is 15-35 DEG C.
In the present invention, the solid-liquid weight in Momordica grosvenori leaching liquor algae-inhibiting agent in wide scope than can select, still
In order that obtaining obtained Momordica grosvenori leaching liquor algae-inhibiting agent has the effect of more excellent suppression algal grown, it is preferable that in step c
In, on the basis of the gross weight of the Momordica grosvenori leaching liquor algae-inhibiting agent, the content of the Grosvenor Momordica Fruit is 8-12 weight %.
Present invention provides a kind of Momordica grosvenori leaching liquor algae-inhibiting agent, Momordica grosvenori leaching liquor algae-inhibiting agent passes through above-mentioned method
It is prepared.
Present invention also offers a kind of answering in the growth for suppressing algae according to above-mentioned Momordica grosvenori leaching liquor algae-inhibiting agent
With.
The present invention will be described in detail by way of examples below.In following examples, Momordica grosvenori is that Wuhu common people are big
The commercially available product in pharmacy；Microcystic aeruginosa liquid：Microcystic aeruginosa PCC7806 (is purchased from Inst. of Hydrobiology, Chinese Academy of Sciences
Algae kind storehouse) to be cultivated to obtain microcystic aeruginosa liquid in BG-11 culture mediums (specific composition is shown in Table 1), condition of culture is：Illumination
Intensity is 4000lx, Light To Dark Ratio 12h：12h, temperature are 24-26 DEG C, and the number being shaken every day is 3-5 times, and culture obtains after 7 days
To microcystic aeruginosa liquid.
Table 1
Embodiment 1
A, Momordica grosvenori is smashed, Grosvenor Momordica Fruit is made in the sieve for crossing 150 mesh；
B, at 25 DEG C, Grosvenor Momordica Fruit is soaked in 3 days obtained Momordica grosvenori suspensions in water；Wherein, Grosvenor Momordica Fruit
Mass ratio with the aqueous solution is 1:20.
C, by the concentration of above-mentioned Momordica grosvenori suspension until solid-liquid weight ratio is 1:Momordica grosvenori leaching liquor algae-inhibiting agent is made when 10
A1。
Comparative example 1
A, Momordica grosvenori is smashed, Grosvenor Momordica Fruit is made in the sieve for crossing 150 mesh；
B, at 4 DEG C, Grosvenor Momordica Fruit is soaked in water 3 days, then degerming (the micropore of filtering with microporous membrane at 25 DEG C
0.22 μm of filter membrane diameter, produce house：Upper Haixing County is sub-) Momordica grosvenori suspension is made；Wherein, the mass ratio of Grosvenor Momordica Fruit and the aqueous solution
For 1:20.
C, by the concentration of above-mentioned Momordica grosvenori suspension until solid-liquid weight ratio is 1:Momordica grosvenori leaching liquor algae-inhibiting agent is made when 10
B1。
Comparative example 2
A, Momordica grosvenori is smashed, Grosvenor Momordica Fruit is made in the sieve for crossing 150 mesh；
B, at 100 DEG C, Grosvenor Momordica Fruit is soaked in water boil 10 minutes and at 121 DEG C sterilize 20min be made
Momordica grosvenori suspension；Wherein, the mass ratio of Grosvenor Momordica Fruit and the aqueous solution is 1:20.
C, by the concentration of above-mentioned Momordica grosvenori suspension until solid-liquid weight ratio is 1:Momordica grosvenori leaching liquor algae-inhibiting agent is made when 10
B1。
Detect example 1
Different amounts of Momordica grosvenori leaching liquor algae-inhibiting agent A1 is added to microcystic aeruginosa liquid and (dilutes and causes microcystic aeruginosa liquid
Initial algae cell density be 5 × 105Cells/ml the quality point of the Momordica grosvenori leaching liquor in microcystic aeruginosa liquid is caused in)
Number is respectively 0,0.04%, 0.08%, 0.12%, 0.16% and 0.2%, is calculated every 24h in a microcystic aeruginosa liquid
Algae cell density, Continuous plus 7 days.Concrete outcome is shown in Fig. 1.As shown in Figure 1, Momordica grosvenori leaching liquor algae-inhibiting agent A1 has to algae
Excellent suppression growth；And the increase of the concentration of the Momordica grosvenori leaching liquor algae-inhibiting agent A1 in microcystic aeruginosa liquid, suppress
Rate is bigger；Times of the Momordica grosvenori leaching liquor algae-inhibiting agent A1 in microcystic aeruginosa liquid is longer, and inhibiting rate is bigger.
Detect example 2
By the microcystic aeruginosa liquid of five parts of normal growths, (algae cell density is 2.2 × 106Cells/ml) it is diluted to respectively
100%, 80%, 60%, 40%, the 20% of former algae solution, Momordica grosvenori leaching liquor is then added into this five parts of microcystic aeruginosa liquid
Algae-inhibiting agent A1 make it that its mass fraction in respective microcystic aeruginosa liquid is 2%.Then respectively 3h, 6h, 1 day, 2 days and
Algae cell density being counted with counting method of blood cell after 3 days and calculating inhibiting rate, concrete outcome is shown in Fig. 3.
From the figure 3, it may be seen that Momordica grosvenori leaching liquor algae-inhibiting agent A1 is 1.2 × 10 to algae cell density6cells/ml-6×
106Cells/ml microcystic aeruginosa liquid is respectively provided with excellent inhibitory action.
Detect example 3
Each blake bottle in example 2 will be detected and remove nutrient solution in centrifugation in the 4th day, the fresh BG-11 of 50ml are then respectively added and cultivate
Base, it is placed in incubator and is cultivated, condition of culture is：Intensity of illumination is 4000lx, Light To Dark Ratio 12h：12h, temperature 24-
26 DEG C, the number being shaken every day is 3-5 times, and calculating algae cell density with blood counting chamber respectively at 1d, 2d, 3d (changes and cultivate
That day of liquid is designated as 0d), wherein, algal control rate (IR)=(1-N/N. with 50ml microcystic aeruginosa liquid as a control group0)×
100%, N be treatment group algae cell density, N0For the algae cell density of control group.The result of specific inhibiting rate is shown in Fig. 3.
From the figure 3, it may be seen that after Momordica grosvenori leaching liquor algae-inhibiting agent acts on microcystic aeruginosa four days, the suppression to microcystic aeruginosa
Also delay effect.
Detect example 4
50ml microcystic aeruginosa liquid is taken in 100ml conical flask, then added into the conical flask it is a certain amount of in
Then herbal medicine algae-inhibiting agent calculates algae cell density as treatment group behind 5 days and 9 days by blood cell plate counting method respectively, wherein
With 50ml microcystic aeruginosa liquid as a control group, algal control rate (IR)=(1-N/N0) × 100%, N is the frustule for the treatment of group
Density, N0For the algae cell density of control group.
Momordica grosvenori leaching liquor algae-inhibiting agent A1, B1 and B2 are added separately in different microcystic aeruginosa liquid (frustule
Initial density is 2.6 × 106Cells/mL (quality of the Momordica grosvenori leaching liquor algae-inhibiting agent in microcystic aeruginosa liquid) is cultivated
Fraction is the 0.3%) inhibiting rate surveyed after culture, wherein, condition of culture is：Intensity of illumination is 4000lx, Light To Dark Ratio 12h：
12h, temperature are 24-26 DEG C, and the number being shaken every day is 3-5 times.Wherein, blank group is to be not added with Momordica grosvenori leaching liquor algae-inhibiting agent
Microcystic aeruginosa liquid.Specific inhibiting rate result is shown in Fig. 4
As shown in Figure 4, the effect of algae restraint of Momordica grosvenori leaching liquor algae-inhibiting agent provided by the invention is unrelated with bacterium, and the arhat
Fruit leaching liquor algae-inhibiting agent has heat endurance.
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, a variety of simple variants can be carried out to technical scheme, this
A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (5)
- A kind of 1. application of Momordica grosvenori leaching liquor algae-inhibiting agent in the growth for suppressing algae, it is characterised in that the Momordica grosvenori leaching The preparation method of extract algae-inhibiting agent includes：A, Momordica grosvenori is crushed and Grosvenor Momordica Fruit is made；B, the Grosvenor Momordica Fruit and water is mixed so that Momordica grosvenori suspension is made；C, the Momordica grosvenori suspension is concentrated and Momordica grosvenori leaching liquor algae-inhibiting agent is made.
- 2. application according to claim 1, wherein, the particle diameter of the Grosvenor Momordica Fruit is not less than 150 mesh.
- 3. application according to claim 1 or 2, wherein, in stepb, relative to the Grosvenor Momordica Fruit of 1 parts by weight, institute The dosage for stating water is 15-25 parts by weight.
- 4. application according to claim 3, wherein, in stepb, the mixing meets following condition：Incorporation time is 2-4 days, mixing temperature was 15-35 DEG C.
- 5. according to the application described in any one in claim 1,2 and 4, wherein, in step c, extracted with the Momordica grosvenori On the basis of the gross weight of liquid algae-inhibiting agent, the content of the Grosvenor Momordica Fruit is 8-12 weight %.
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| CN201510007221.6A CN104604950B (en) | 2015-01-07 | 2015-01-07 | Momordica grosvenori leaching liquor algae-inhibiting agent and its preparation method and application |
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| CN112544640A (en) * | 2020-12-15 | 2021-03-26 | 安徽师范大学 | Algae inhibitor derived from sugarcane peel and preparation method thereof |
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| 中药罗汉果对小鼠急性毒性和致突变性研究;艾丽萍，高顺;《安徽农业科学》;20140120;774-775 * |
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