CN106966472A - A kind of preparation method of sewage bacteriophage inhibitor and application - Google Patents
A kind of preparation method of sewage bacteriophage inhibitor and application Download PDFInfo
- Publication number
- CN106966472A CN106966472A CN201710295582.4A CN201710295582A CN106966472A CN 106966472 A CN106966472 A CN 106966472A CN 201710295582 A CN201710295582 A CN 201710295582A CN 106966472 A CN106966472 A CN 106966472A
- Authority
- CN
- China
- Prior art keywords
- water
- aqueous
- preparation
- extracted
- bacteriophage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to water treatment field, and in particular to a kind of preparation method of sewage bacteriophage inhibitor.It will be extracted first after water pepper material disintegrating with absolute ethyl alcohol, extract obtained with 2-5 liters of absolute ethyl alcohols with per kilogram water pepper material；The extract is concentrated to give paste again and is dissolved in water, supernatant is taken；Supernatant takes aqueous-phase material after being layered with petroleum ether extraction；It is extracted with chloroform again, fetches water and is mutually extracted with ethyl acetate after layering, aqueous phase continues to use saturation extracting n-butyl alcohol, and the saturation n-butanol of gained is mutually.Obviously, convenient material drawing of the present invention, cost is low, rationally effective and less energy consumption, non-secondary pollution, is particularly suitable for being applied to water body in large sterilization.
Description
Technical field
The invention belongs to preparation method and the application of water treatment field, more particularly to a kind of sewage bacteriophage inhibitor.
Background technology
The sterilization method commonly used in current sewage disposal is many, such as adds chlorine-containing disinfectant, ultraviolet irradiation and film and cuts
Stay, its expense is of a relatively high, therefore be unsuitable for water body in large sterilization.Wherein chlorine-containing disinfectant is used for drinking water and disappeared
Poison, but due to content of organics height in sewage, DBPs such as is also easy to produce using chlorine-containing disinfectant, endanger environmental health.If
The method irradiated using ultraviolet, then due to decaying in water quickly, sterilization scope is smaller, if for wastewater disinfection, needed
A large amount of uviol lamps are installed, mass energy is expended.And film retention method is easily blocked due to filter membrane, operation needs elevated pressures, energy consumption
Also it is higher.
Numerous studies show that certain plants can produce other harmful microbe growth substances around suppression.Wherein there is water
The water plants such as knotweed, reed, cattail, calamus, giantreed, but suppress to bite using water plant extract solution in water environment field at present
The research of thalline is not yet found.Therefore the material for wherein suppressing bacteriophage is separated, to be used as sewage bacteriophage inhibitor
It is very important.
The content of the invention
It is an object of the invention to provide a kind of preparation method of sewage bacteriophage inhibitor and application, to make up prior art
Deficiency.
Water pepper is annual herb, high 20~80 centimetres, and upright or bottom is thrown oneself on the ground, and is mainly grown in water or bank.Water pepper exists
China is widely distributed, is the southern and northern common species being distributed.The present invention is by lot of experiments, from water pepper, reed, perfume (or spice)
The water pepper for having better inhibition effect to bacteriophage is filtered out in the water plants such as Pu, calamus, giantreed, watermifoil, stonewort, eel grass,
And the material for suppressing bacteriophage in water pepper is separated, sewage bacteriophage inhibitor is made with efficiently and targetedly
Applied to larger area water body disinfection.
The method of the present invention comprises the following steps：
1）Extracted 1-8 hours with absolute ethyl alcohol after water pepper plant is crushed, be filtrated to get extract solution；
2）Said extracted liquid is carried out to be concentrated to give paste, then paste is dissolved with distilled water, supernatant is taken；
3）By above-mentioned supernatant petroleum ether extraction, aqueous-phase material is taken；
4）The aqueous-phase material is extracted with chloroform again, aqueous-phase material is taken；
5）Above-mentioned aqueous-phase material continues to be extracted with ethyl acetate, and takes aqueous-phase material；
6）By step 5）Aqueous-phase material saturation extracting n-butyl alcohol, takes n-butanol phase, and gained n-butanol is mutually to suppress
The bacteriophage inhibitor of bacteriophage growth.
In step 1）Middle per kilogram water pepper material rises absolute ethyl alcohol with 2-5 and extracted.
In step 3）Described in supernatant use 1:1-1:5 petroleum ether is extracted.
In step 4）Middle chloroform：The volume ratio of aqueous phase is 1:1-1:20.
In step 5）Middle ethyl acetate：The volume ratio of aqueous phase is 1:1-1:5.
In step 6）Described in saturation n-butanol：The volume ratio of aqueous phase is 1:1-1:5.
The present invention is applied to the bacteriophage sterilization in water body in large.Including MS2, T4 and f2 bacteriophage etc..
The concentration applied using the inhibitor of the present invention when in the water containing bacteriophage is 1-2 mg/litres, especially to bacteriophage
Inhibiting rate is optimal.
Obviously, it is characteristic of the invention that convenient material drawing cost is low, reasonable available energy dissipation is few, and non-secondary pollution is particularly suitable for
Applied to water body in large sterilization, generally up to more than 96%.
Embodiment
The present invention, which can select mutually reply bacteriophage on the spot, reed, cattail, calamus, giantreed, the fox of better inhibition effect
The water plants such as tail algae, stonewort, eel grass specially therefrom filter out water pepper, and carried out effective thing as material, the present invention
Change is handled, i.e., the method that separation bacteriophage inhibitor is extracted from water pepper is as follows：
1）Water pepper is extracted 1-8 hours after crushing with absolute ethyl alcohol, filters to obtain extract solution, usual per kilogram water pepper is anhydrous with 2-5 liters
Ethanol；
2）Extract solution is concentrated using the method such as reduction vaporization, dry, obtains paste.Paste distilled water dissolves, and uses
Centrifuge is centrifuged 10 minutes under the conditions of 5000 revs/min, is obtained supernatant and precipitation two parts, is taken supernatant；
3）Supernatant is extracted with petroleum ether, phase of fetching water；
4）Above-mentioned aqueous phase is extracted with chloroform, phase of fetching water；
5）Above-mentioned gained aqueous phase makes to be extracted with ethyl acetate, phase of being fetched water after layering；
6）Above-mentioned gained aqueous phase is used into saturation extracting n-butyl alcohol, obtained saturation n-butanol is mutually to suppress bacteriophage growth
Bacteriophage inhibitor.
The embodiment of the present invention is further illustrated below by embodiment.
Embodiment 1
500 grams of water peppers after crushed are extracted with 1 liter of absolute ethyl alcohol, then extract solution distilled water is dissolved, then by supernatant
Respectively successively with 1:1 petroleum ether, 1:1 chloroform, 1:1 ethyl acetate and saturation 1:1 extracting n-butyl alcohol, obtained saturation
N-butanol phase solution is bacteriophage inhibitor.
Bacteriophage inhibitor obtained above is added in the water containing bacteriophage, when concentration is 1 ml l, T4 bitten
The inhibiting rate of thalline is 97%.
Embodiment 2
1 kilogram of water pepper after crushing is extracted with 5 liters of absolute ethyl alcohols, dissolved after its extract solution is concentrated with distilled water, afterwards
Supernatant respectively successively with 1:5 petroleum ether, 1:5 chloroform, 1:3 ethyl acetate and saturation 1:2 extracting n-butyl alcohol, is obtained
To saturation n-butanol phase solution be bacteriophage inhibitor.Suppress the growth of water pnagus medius using the bacteriophage inhibitor,
When concentration is 2 ml l, the inhibiting rate to T4 bacteriophages is 100%.
Embodiment 3
1 kilogram of water pepper after crushing is extracted with 2 liters of absolute ethyl alcohols, dissolved after its extract solution is concentrated with distilled water, afterwards
Supernatant respectively successively with 1:1 petroleum ether, 1:20 chloroform, 1:1 ethyl acetate and saturation 1:1 extracting n-butyl alcohol,
Obtained saturation n-butanol phase solution is bacteriophage inhibitor.Using the bacteriophage inhibitor, when concentration is 2 ml l,
Suppress the growth of water pnagus medius, to the inhibiting rates of f2 bacteriophages up to 100%.
Embodiment 4
1 kilogram of water pepper after crushing is extracted with 5 liters of absolute ethyl alcohols, dissolved after its extract solution is concentrated with distilled water, supernatant
Liquid is respectively successively with 1:5 petroleum ether, 1:5 chloroform, 1:3 ethyl acetate and saturation 1:2 extracting n-butyl alcohol, what is obtained is full
It is bacteriophage inhibitor with n-butanol phase solution.Suppress the growth of water pnagus medius using the bacteriophage inhibitor, work as concentration
During for 2 ml l, the inhibiting rate to f2 bacteriophages is 96%.
Embodiment 5
1 kilogram of water pepper after crushing is extracted with 2 liters of absolute ethyl alcohols, dissolved after its extract solution is concentrated with distilled water, supernatant
Liquid is respectively successively with 1:1 petroleum ether, 1:5 chloroform, 1:1 ethyl acetate and saturation 1:2 extracting n-butyl alcohol, what is obtained is full
It is bacteriophage inhibitor with n-butanol phase solution.Suppress the growth of water pnagus medius using the bacteriophage inhibitor, work as concentration
During for 2 ml l, the inhibiting rate to MS2 bacteriophages is 96%.
Obviously, the present invention has at a relatively high inhibiting rate to T4 bacteriophages, f2 bacteriophages and MS2, i.e., to bacteriophage（Virus
Substitute）Inhibiting rate is preferred.
Claims (8)
1. a kind of preparation method of sewage bacteriophage inhibitor, is characterised by that this method comprises the following steps：
1）Extracted 1-8 hours with absolute ethyl alcohol after water pepper plant is crushed, be filtrated to get extract solution；
2）Said extracted liquid is carried out to be concentrated to give paste, then paste is dissolved with distilled water, supernatant is taken；
3）By above-mentioned supernatant petroleum ether extraction, aqueous-phase material is taken；
4）The aqueous-phase material is extracted with chloroform again, aqueous-phase material is taken；
5）Above-mentioned aqueous-phase material continues to be extracted with ethyl acetate, and takes aqueous-phase material；
6）By step 5）Aqueous-phase material saturation extracting n-butyl alcohol, takes n-butanol phase, and gained n-butanol is mutually to suppress
The bacteriophage inhibitor of bacteriophage growth.
2. preparation method according to claim 1, it is characterised in that：In step 1）Middle per kilogram water pepper rises anhydrous with 2-5
Alcohol steep.
3. preparation method according to claim 1, it is characterised in that step 3）Described in supernatant use 1:1—1:5
Petroleum ether extracted.
4. preparation method according to claim 1, it is characterised in that step 4）Middle chloroform：The volume ratio of aqueous phase is 1:1-1:
20。
5. preparation method according to claim 1, it is characterised in that step 5）Middle ethyl acetate：The volume ratio of aqueous phase is 1:
1-1:5。
6. preparation method according to claim 1, it is characterised in that step 6）Described in saturation n-butanol：The volume of aqueous phase
Than for 1:1-1:5.
7. the bacteriophage inhibitor prepared with the preparation method of claim 1, it is characterised in that applied in water body in large
Bacteriophage sterilizes.
8. bacteriophage inhibitor prepared by preparation method as claimed in claim 1, it is characterised in that apply to contain using the inhibitor and bite
Concentration when in the water of thalline is 1-2 mg/litres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710295582.4A CN106966472A (en) | 2017-04-28 | 2017-04-28 | A kind of preparation method of sewage bacteriophage inhibitor and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710295582.4A CN106966472A (en) | 2017-04-28 | 2017-04-28 | A kind of preparation method of sewage bacteriophage inhibitor and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106966472A true CN106966472A (en) | 2017-07-21 |
Family
ID=59331101
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710295582.4A Pending CN106966472A (en) | 2017-04-28 | 2017-04-28 | A kind of preparation method of sewage bacteriophage inhibitor and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106966472A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771483A (en) * | 2019-03-18 | 2019-05-21 | 泉州师范学院 | A method of extracting high activity alpha-glucosidase restrainer from water polygonum flaccidum |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799367A (en) * | 2003-11-07 | 2006-07-12 | 清华大学 | Method for inhibiting algae growth by reed |
| CN101897347A (en) * | 2010-07-09 | 2010-12-01 | 华南农业大学 | Plant-based bacteriostat, preparation method thereof and application thereof |
| CN104083453A (en) * | 2014-07-31 | 2014-10-08 | 湖南科技学院 | Extraction method for total flavonoids in polygonum hydropiper |
| CN105360171A (en) * | 2015-10-12 | 2016-03-02 | 邯郸学院 | Preparation method and application of plant source bacteriostatic agent |
-
2017
- 2017-04-28 CN CN201710295582.4A patent/CN106966472A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799367A (en) * | 2003-11-07 | 2006-07-12 | 清华大学 | Method for inhibiting algae growth by reed |
| CN101897347A (en) * | 2010-07-09 | 2010-12-01 | 华南农业大学 | Plant-based bacteriostat, preparation method thereof and application thereof |
| CN104083453A (en) * | 2014-07-31 | 2014-10-08 | 湖南科技学院 | Extraction method for total flavonoids in polygonum hydropiper |
| CN105360171A (en) * | 2015-10-12 | 2016-03-02 | 邯郸学院 | Preparation method and application of plant source bacteriostatic agent |
Non-Patent Citations (2)
| Title |
|---|
| FENGMIN LI等: "A novel method of rural sewage disinfection via root extracts of hydrophytes", 《ECOLOGICAL ENGINEERING》 * |
| 张男男: "水生植物化感物质对噬菌体的抑制作用", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑（月刊）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109771483A (en) * | 2019-03-18 | 2019-05-21 | 泉州师范学院 | A method of extracting high activity alpha-glucosidase restrainer from water polygonum flaccidum |
| CN109771483B (en) * | 2019-03-18 | 2021-11-02 | 泉州师范学院 | Method for extracting high-activity alpha-glucosidase inhibitor from polygonum hydropiper |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5269160B2 (en) | Bioactive liquid composition and method for producing the same | |
| Pritchard et al. | Potential of using plant extracts for purification of shallow well water in Malawi | |
| Dorea | Use of Moringa spp. seeds for coagulation: a review of a sustainable option | |
| CN102415416B (en) | Method for preparing alga inhibitor from barley | |
| CN101906353A (en) | Extraction method and application of chestnut flower essential oil | |
| Pandit et al. | Drinking water treatment for developing countries: physical, chemical and biological pollutants | |
| JP4820662B2 (en) | Fish parasite anthelmintic composition using natural plant extract {Anthelistic composition forfishing parasiticing natural plant tracts} | |
| CN106966472A (en) | A kind of preparation method of sewage bacteriophage inhibitor and application | |
| CN101843261B (en) | Method and application for extracting alexandrium tamarense inhibitor from enteromorpha | |
| CN107318894B (en) | The inhibitor and its preparation and application administered for microcystic aeruginosa | |
| CN106955583A (en) | The preparation method of biology enzyme/plant extraction liquid degradation of formaldehyde composite | |
| CN105360186A (en) | Bamboo juice disinfectant and preparation method and application thereof | |
| CN101462779B (en) | Preparation of Gymnodinium breve inhibitor | |
| CN101289240A (en) | Novel finishing agent for removing algal tufa and treatment method | |
| CN1850630A (en) | Method for preparing enteromorpha extract for inhibiting red tide algae and its use | |
| CN106577844B (en) | Prevent and treat capsicum epidemic disease preparation, its preparation and application | |
| KR101829622B1 (en) | Eco-friendly composition for removing green algae having function of deodorization and sterilization and removing method for green algae using the same | |
| CN106719926A (en) | A kind of preparation technology of pure Chinese medicine herbicide | |
| CN104522794B (en) | Antrodia camphorata detoxifying beverage and preparation method thereof | |
| Kubendran | WASH behavior: Indigenous method for Water Purification in rural India | |
| CN101891275A (en) | Method for controlling microcystis waterbloom | |
| CN205953689U (en) | Sewage treatment mechanism | |
| CN105248467B (en) | A method of prevent archives from going mouldy and damaging by worms | |
| Oloruntade et al. | A case for the use of Moringa olifera as a natural coagulant to improve water supply in rural farms in Nigeria | |
| CN102745814A (en) | Preparation method for barley decomposed solution used for controlling harmful algae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170721 |
|
| WD01 | Invention patent application deemed withdrawn after publication |