CN101843261B - Method and application for extracting alexandrium tamarense inhibitor from enteromorpha - Google Patents
Method and application for extracting alexandrium tamarense inhibitor from enteromorpha Download PDFInfo
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- CN101843261B CN101843261B CN 201010173006 CN201010173006A CN101843261B CN 101843261 B CN101843261 B CN 101843261B CN 201010173006 CN201010173006 CN 201010173006 CN 201010173006 A CN201010173006 A CN 201010173006A CN 101843261 B CN101843261 B CN 101843261B
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- enteromorpha
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- alexandrium tamarense
- ethyl acetate
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Abstract
The invention relates to a method and an application for extracting an alexandrium tamarense inhibitor from enteromorpha. The method comprises the following steps of: lixiviating per kilogram of the enteromorpha by using 2-5 liters of distilled water at room temperature for 12-72 hours, filtering to take filter liquor, and concentrating the obtained filter liquor into pastes by adopting a rotary evaporation method; dissolving the concentrated pastes by using a mixing solution of petroleum ether and 90 percent of alcohol which are in a 1:1 ratio; sufficiently oscillating for 1-3 minutes, then demixing by standing for 10-120 minutes, and taking a lower-layer alcohol phase; decompressing, evaporating and concentrating the alcohol phase to obtain a paste-like substance at 40 DEG C; extracting and separating by using ethyl acetate and secondary distilled water which are in the 1:1 ratio, wherein a ethyl acetate phase is the alexandrium tamarense inhibitor; and determining the volume of the alexandrium tamarense inhibitor extracted by per kilogram of the enteromorpha to 50 milliliters. The invention is used for inhibiting the growth of alexandrium tamarense by adding 0.5-5 milliliters of the alexandrium tamarense inhibitor to per cubic metre of a water body, sufficiently utilizes the enteromorpha, has efficient economy, good ecological safety and easy operation and control, effectively inhibits the growth of the oceanic alexandrium tamarense and particularly inhibits the growth of gymnodinium breve.
Description
Technical field
The invention belongs to the Seawater Treatment field, relate in particular to a kind of methods and applications that extract alexandrium tamarense inhibitor from Enteromorpha.
Background technology
Enteromorpha (Enteromorpha prolifra (Muell.) J.Ag.) is also named tongue bar, green moss, is a kind of Common Marine Macrophytes.The frond grass green, tubulose is membranous, grows thickly, and major branch is obvious, and branch is elongated, 1 meter of Gao Keda.Since 2006, annual summer, Huanghai Sea sea is assembled, floated on to a large amount of Enteromorphas, forms the green tide of large tracts of land on Along North Jiangsu, South Coasts of Shandong Peninsula and other places.Especially in June, 2008, a large amount of Enteromorphas drift to the surrounding waters, Qingdao from the Central Yellow Sea area, and Qingdao Area marine site and bank have met with and arised suddenly, historical rare Enteromorpha natural calamity.When the highest, distribution area reaches 2.4 ten thousand square kilometres.The Olympic Sailing competition field marine of 60 square kilometres is covered by 1/3 Enteromorpha, and every day with the quick-fried length of 10% speed, once yachtsman's sea training was impacted.To July 15, through tens thousand of the army and the people's salvaging, remove altogether more than 100 ten thousand tons of Enteromorphas.
Although the Enteromorpha in Olympic Sailing competition place has been cleaned out, the biomass that the Enteromorpha moisture content is high, salinity is many, the short time domestic demand is processed is large, the huge difficult problem that the disposal of more than 100 ten thousand tons of Enteromorphas salvaging but brings.How with the Enteromorpha recycling, when reducing disposal costs, producing certain economic benefit will be the improvement generation positive impact of ocean green tide.In addition, because the discharging of Lu Yuan nutriment and ocean operation and the pollutant that leaks enter the ocean in a large number, cause the seawater eutrophication.Eutrophication causes the explosive growth of red tide algae, brings massive losses for the marine eco-environment, culture fishery, tourist industry etc.Some algae can also produce the algae toxin, brings harm for aquatic animal and the mankind's health.Many tangleweeds can produce the allelochemical that suppresses the red tide algae growth.Gymnodinium breve is coastal common and main red tide algae of Eastern China, therefore usually with the representative of Gymnodinium breve as red tide algae.
Existing alexandrium tamarense inhibitor is mainly copper ion preparation and artificial synthetic organic herbicide.Utilize now copper ion (Cu
2+) to kill red tide algae be to use the most ripe a kind of red tide inhibition technology, but copper ion can not degrade in water body, can transmit in the ecosystem by food chain, and copper ion concentration higher (biomagnification) in the higher organism of trophic level.If the people has eaten the enrichment aquatic animal of copper ion, meeting serious harm health.The artificial weeding agent can be killed other plant in marine ecosystems when killing red tide algae, make water ecosystem destroyed, and ecological functions disappear, and finally make the ocean become " Dead Sea " that biomass sharply descends.Therefore, need that exploitation is efficient badly, the red tide inhibitor of safety, easy operation control.
Summary of the invention
The purpose of this invention is to provide a kind of methods and applications that extract alexandrium tamarense inhibitor from Enteromorpha, for the efficient inhibition of Enteromorpha recycling, red tide algae provides technical support, to overcome the deficiency of prior art.
The present invention passes through lot of experiments, Enteromorpha and other multiple tangleweed have been compared to the inhibition ability of Gymnodinium breve, result of study shows that Enteromorpha can significantly suppress the growth of Gymnodinium breve, therefore extract from Enteromorpha and have the algal control material that suppresses the red tide algae growing ability, and it is dropped in seawater suppress red tide algae, the particularly growth of Gymnodinium breve.
The method of extracting alexandrium tamarense inhibitor from Enteromorpha is:
(1) Enteromorpha is cleaned natural air drying.The per kilogram Enteromorpha rises distilled water under room temperature lixiviate 12-72 hour with 2-5, crosses leaching filtrate, adopts the rotary evaporation method to be concentrated into paste the filtrate that obtains;
(2) with benzinum and the 90% alcohol mixeding liquid dissolving of condensed cream with 1: 1, after fully vibrating 1-3 minute, standing 10-120 minute layering, the ethanol phase of taking off layer;
(3) ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtains pasty masses, uses again ethyl acetate and the redistilled water extract and separate of 1: 1, and wherein ethyl acetate is alexandrium tamarense inhibitor mutually, and the inhibitor that the per kilogram Enteromorpha is extracted is settled to 50 milliliters.
During use, be applied to suppress the growth of red tide algae to add 0.5-5 milliliter alexandrium tamarense inhibitor in every cubic metre of water body.
The present invention takes full advantage of the Enteromorpha in the Enteromorpha plant, and economical and efficient, ecological security are good, easy operation control, effectively suppress the particularly growth of Gymnodinium breve of ocean harmful algae.
Embodiment
The embodiment of the present invention describes the present invention in detail with the representative of Gymnodinium breve as red tide algae.
The preparation method of the enteromorpha extract of inhibition red tide algae of the present invention is: 1 kilogram of Enteromorpha is cleaned natural air drying.The per kilogram Enteromorpha rose the distilled water lixiviate 12-48 hour with 2-5, obtained extract with the method for filtering, with the concentrated condensed cream that forms of extract.With benzinum and the 90% alcohol mixeding liquid dissolving of condensed cream with 1: 1.Fully vibration, standing demix takes off layer (ethanol phase).Ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtains pasty masses, with 80 milliliters of ethyl acetate of 1: 1 and redistilled water extract and separate.Ethyl acetate is alexandrium tamarense inhibitor (amount to obtain is about 40 milliliters) mutually.The inhibitor that obtains is applied in staying water, and every liter of staying water drops into 0.2-2 milliliter inhibitor.
Below by the furthermore bright the present invention of a plurality of embodiment.
Embodiment 1
Get 0.1 kilogram of Enteromorpha and filter after 24 hours with 0.3 liter of distilled water immersion, filtrate is concentrated.
Condensed cream is dissolved with 40 milliliters of benzinums of 1: 1 and 90% alcohol mixeding liquid.Fully vibration, standing demix takes off layer (ethanol phase).Ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtains pasty masses, with 20 milliliters of ethyl acetate of 1: 1 and redistilled water extract and separate.Ethyl acetate is alexandrium tamarense inhibitor (amount to obtain is about 10 milliliters) mutually.
Get inhibitor 0.1,0.2,0.3,0.4,0.5 milliliter adds respectively in 200 milliliters of typical algae Gymnodinium breve culture fluids, and after 3 days, inhibiting rate is respectively 65%, 86%, and 96%, 98%, 99.5%.
Embodiment 2
1 kilogram of Enteromorpha filters after 48 hours with 2 liters of distilled water immersions, and filtrate is concentrated.
Condensed cream is dissolved with 100 milliliters of benzinums of 1: 1 and 90% alcohol mixeding liquid.Fully vibration, standing demix takes off layer (ethanol phase).Ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtains pasty masses, with 100 milliliters of ethyl acetate of 1: 1 and redistilled water extract and separate.Ethyl acetate is alexandrium tamarense inhibitor (amount to obtain is about 50 milliliters) mutually.
Get inhibitor 0.2,0.3,0.4,0.5,1 milliliter adds respectively in 100 milliliters of typical algae Gymnodinium breve culture fluids, and after 3 days, inhibiting rate is respectively 81%, 92%, and 97%, 99%, 99%.
Embodiment 3
1 kilogram of Enteromorpha filters after 24 hours with 5 liters of distilled water immersions, and filtrate is concentrated.
Condensed cream is dissolved with 100 milliliters of benzinums of 1: 1 and 90% alcohol mixeding liquid.Fully vibration, standing demix, the ethanol phase of taking off layer.Ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtains pasty masses, with 100 milliliters of ethyl acetate of 1: 1 and redistilled water extract and separate.Ethyl acetate is alexandrium tamarense inhibitor (amount to obtain is about 50 milliliters) mutually.
Get inhibitor 0.2,0.3,0.4,0.5,1 milliliter adds respectively in 100 milliliters of typical algae Gymnodinium breve culture fluids, and after 2 days, inhibiting rate is respectively 76%, 82%, and 87%, 95%, 97%.
Claims (2)
1. extract the method for alexandrium tamarense inhibitor from Enteromorpha, the per kilogram Enteromorpha rises distilled water under room temperature lixiviate 12-72 hour with 2-5, cross leaching filtrate, adopt the rotary evaporation method to be concentrated into paste the filtrate that obtains, then with benzinum and the 90% alcohol mixeding liquid dissolving of condensed cream with 1:1; Fully vibration is after 1-3 minute, standing 10-120 minute layering is also taken off a layer ethanol phase, ethanol is concentrated at 40 ℃ of lower reduction vaporizations, obtain pasty masses, use again ethyl acetate and the redistilled water extract and separate of 1:1, ethyl acetate is alexandrium tamarense inhibitor mutually, and the inhibitor that the per kilogram Enteromorpha is extracted is settled to 50 milliliters.
2. the alexandrium tamarense inhibitor that is extracted with the method that adds 0.5-5 milliliter claim 1 in every cubic metre of water body is applied to suppress the growth of red tide algae.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010173006 CN101843261B (en) | 2010-05-05 | 2010-05-05 | Method and application for extracting alexandrium tamarense inhibitor from enteromorpha |
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| CN 201010173006 CN101843261B (en) | 2010-05-05 | 2010-05-05 | Method and application for extracting alexandrium tamarense inhibitor from enteromorpha |
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| CN101843261A CN101843261A (en) | 2010-09-29 |
| CN101843261B true CN101843261B (en) | 2013-05-08 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102168039B (en) * | 2010-12-10 | 2012-11-14 | 厦门大学 | Method for screening extracellular algae-killing protein marine bacteria |
| CN103725033B (en) * | 2013-12-24 | 2015-05-27 | 上海海洋大学 | Method for extracting Enteromorpha pigment |
| CN104609521B (en) * | 2014-12-24 | 2016-08-24 | 泰山学院 | A kind of ailanthus altissima (mill.) swingle bark algae-inhibiting agent and preparation method and application |
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
| CN110063430B (en) * | 2019-05-14 | 2022-09-09 | 宁波大学 | Brown algae extract and application of brown algae extract in inhibiting proliferation of virulent Carlo algae in bait microalgae propagation |
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