CN100383055C - Method for preparing inhibitor of skeletonema costatum (greville) cleve - Google Patents
Method for preparing inhibitor of skeletonema costatum (greville) cleve Download PDFInfo
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- CN100383055C CN100383055C CNB2006100445886A CN200610044588A CN100383055C CN 100383055 C CN100383055 C CN 100383055C CN B2006100445886 A CNB2006100445886 A CN B2006100445886A CN 200610044588 A CN200610044588 A CN 200610044588A CN 100383055 C CN100383055 C CN 100383055C
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Abstract
The present invention relates to a method for preparing an inhibitor of skeletonema costatum, which belongs to the field of water treatment. The preparation method of ulva linza extracting solutions used for inhibiting skeletonema costatum comprises the following steps: leaching ulva linza by sterilized seawater, ethyl alcohol, propanone, ethyl ether or chloroform; concentrating the obtained extracting solution with a rotary evaporation method; putting the concentrated extracting solution into a water body to be treated for inhibiting the growth of red tide algae in the water body to be treated. In general, per kilogram ulva linza is leached for 0.5 to 2 days by 30 to 50 liters of seawater; the obtained extracting solution is concentrated to obtain a concentrated solution whose volume is 10% of the original volume, and then the concentrated solution is put into the water body to be treated; 2 to 10 milliliters of the concentrated solution is put into per liter water body to be treated. The present invention makes full use of the ulva linza in ulva linza plants, has the advantages of high efficiency, safety and easy operation and control, effectively inhibits the growth of harmful red tide algae in oceans, especially the growth of skeletonema costatum, and is widely used the in the water bodies of coast areas, ports, bathing beaches, aquaculture bases, etc. for controlling red tides.
Description
Technical field
The invention belongs to water treatment field, relate in particular to a kind of preparation method of inhibitor of middle Skeletonemacostatum.
Background technology
After Lu Yuan nutritive substance and ocean operation discharging and the pollutent that leaks enter ocean water body, causing the seawater eutrophication, is the major cause that ocean environment worsens.Eutrophication causes the explosive growth of red tide algae, can reduce the dissolved oxygen of seawater, brings massive losses for the aquatic products aquaculture.Some algae can also produce the algae toxin, and feed water lively thing and human beings'health are brought harm.Middle Skeletonemacostatum is the coastal common and main red tide algae in Eastern China, therefore usually with the representative of middle Skeletonemacostatum as the red tide algae.
Existing alexandrium tamarense inhibitor is mainly the organic herbicide of cupric ion preparation and synthetic.Utilize cupric ion (Cu now
2+) to kill the red tide algae be to use the most sophisticated a kind of red tide inhibition technology, but cupric ion in water body, can not degrade, can in the ecosystem, transmit by food chain, and copper ion concentration high more (the biological amplification) in the high more organism of trophic level.If the people has eaten the enrichment hydrocoles of cupric ion, meeting serious harm HUMAN HEALTH.The artificial weeding agent can be killed other plant in the Marine ecosystems when killing the red tide algae, make water ecosystem destroyed, and ecological functions disappear, and finally makes the ocean become " Dead Sea " that biomass sharply descends.Therefore, need that exploitation is efficient badly, the red tide inhibitor of safety, easy operation control.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of inhibitor of middle Skeletonemacostatum is to remedy the deficiencies in the prior art.
The present invention is by lot of experiments, and filtering out from multiple kelp has remarkable inhibiting feldspar water shield to the red tide algae, and then has the extracting solution of algal control function to drop into inhibition red tide algae growth in the seawater it.Therefore the extracting solution of feldspar water shield can be used as the application that suppresses the red tide algal grown.
The preparation method of the feldspar water shield extracting solution of Skeletonemacostatum is in the inhibition: with solvent is sterilization seawater lixiviate feldspar water shield, then the extracting solution that obtains is concentrated into the 2%-10% of original volume, extracting solution after concentrating is dropped into staying water, to suppress the growth of red tide algae in the staying water.Per kilogram feldspar water shield rose the seawater lixiviate 0.5-2 days with 30-50 in the said extracted technology, and the extracting solution that obtains is concentrated into the 2%-10% of original volume, applied in the staying water, and every liter of staying water drops into 2-10 milliliter concentrated solution.
The also available organic solvent of above-mentioned solvent, as ethanol, acetone, ether, chloroform or methyl alcohol etc., per kilogram feldspar water shield rose ethanol, acetone, ether, chloroform or methyl alcohol lixiviate 6-48 hour with 2-5, after the extracting solution that obtains is concentrated into the 10%-20% of original volume, apply in the staying water, every liter of staying water drops into 1-5 milliliter concentrated extracting solution.
Therefore, the extracting solution of feldspar water shield is as the application that suppresses the red tide algae.
The present invention makes full use of the feldspar water shield in the feldspar water shield plant, and economical and efficient, good, the easy operation control of ecological security effectively suppress the particularly growth of middle Skeletonemacostatum of ocean harmful algal algae.
Embodiment
The embodiment of the invention describes the present invention in detail with the representative of middle Skeletonemacostatum as the red tide algae.
The preparation method of the feldspar water shield extracting solution of Skeletonemacostatum is in the inhibition of the present invention: with sterilization seawater lixiviate feldspar water shield, adopt the rotary evaporation method to be concentrated into the 2%-10% of original volume the extracting solution that obtains, extracting solution after concentrating is dropped into staying water, to suppress the growth of red tide algae in the staying water.Another kind of scheme is: per kilogram feldspar water shield rises sterilization seawater lixiviate 0.5-2 days with 30-50, and the extracting solution that obtains is concentrated into the 10%-20% of original volume, applies in the staying water, and every liter of staying water drops into 2-10 milliliter concentrated solution.Another kind of scheme is: per kilogram feldspar water shield rose ethanol, acetone, ether, chloroform or methyl alcohol lixiviate 6-48 hour with 2-5, apply in the staying water after the extracting solution that obtains being concentrated into the 10%-20% of original volume, every liter of staying water drops into 1-5 milliliter concentrated extracting solution.Said extracted liquid can adopt rotary evaporation method etc. to concentrate.
Below by the furthermore bright the present invention of a plurality of embodiment.
Embodiment 1
0.1 kilogram feldspar water shield is with 3 liters of 1 day after-filtration of sea water immersion of sterilizing, filtrate is concentrated into 300 milliliters.Get concentrated solution 1,2,3,4,5 milliliters add respectively in 200 milliliters in the Skeletonemacostatum nutrient solution, and after 5 days, inhibiting rate is respectively 65%, 86%, and 96%, 987%, 99.5%.
Embodiment 2
1 kilogram of feldspar water shield is with 12 hours after-filtration of 2 liters of extraction using alcohols, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and this algae density only is 3% of control group after 5 days, and promptly inhibiting rate is 97%.
1 kilogram of feldspar water shield is with 48 hours after-filtration of 2 liters of extraction using alcohols, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 2% of control group after 5 days, and promptly inhibiting rate is 98%.
Embodiment 3
1 kilogram of feldspar water shield is with 12 hours after-filtration of 2 liters of acetone extractions, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 3% of control group after 5 days, and promptly inhibiting rate is 97%.
1 kilogram of feldspar water shield is with 24 hours after-filtration of 2 liters of acetone extractions, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 2% of control group after 5 days, and promptly inhibiting rate is 98%.
1 kilogram of feldspar water shield is with 48 hours after-filtration of 2 liters of acetone extractions, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 1.5% of control group after 5 days, and promptly inhibiting rate is 98.5%.
1 kilogram of feldspar water shield is with 48 hours after-filtration of 5 liters of acetone extractions, and filtrate is concentrated into 500 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 4% of control group after 5 days, and promptly inhibiting rate is 96%.
Embodiment 4
1 kilogram of feldspar water shield is with 12 hours after-filtration of 2 liters of ether extraction, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 2.5% of control group after 5 days, and promptly inhibiting rate is 97.5%.
1 kilogram of feldspar water shield is with 24 hours after-filtration of 2 liters of chloroform extraction, and filtrate is concentrated into 200 milliliters, and the extracting solution that obtains is applied Skeletonemacostatum nutrient solution in 200 milliliters for 0.5 milliliter, and algae density only is 1% of control group after 5 days, and promptly inhibiting rate is 99%.
Claims (4)
1. the preparation method of the inhibitor of Skeletonemacostatum in a kind, at first with sterilization seawater lixiviate feldspar water shield, then the extracting solution that obtains is concentrated into the 2%-10% of original volume, the extracting solution after will concentrating again drops into the growth of 2-10 milliliter with Skeletonemacostatum in suppressing by every liter of staying water.
2. preparation method as claimed in claim 1 is characterized in that above-mentioned extracting technology is to rise sterilization seawater lixiviate 0.5-2 days with per kilogram feldspar water shield with 30-50.
3. the preparation method of the inhibitor of Skeletonemacostatum in a kind, it is characterized in that at first using etoh solvent, acetone, ether, chloroform or methyl alcohol lixiviate feldspar water shield, per kilogram feldspar water shield rose ethanol, acetone, ether, chloroform or methyl alcohol lixiviate 6-48 hour with 2-5, after then the extracting solution that obtains being concentrated into the 10%-20% of original volume, apply in the staying water, every liter of staying water drops into 1-5 milliliter concentrated extracting solution again.
4. the application of the feldspar water shield extracting solution of Skeletonemacostatum in suppressing is characterized in that the application of the extracting solution of feldspar water shield as Skeletonemacostatum in suppressing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100445886A CN100383055C (en) | 2006-05-26 | 2006-05-26 | Method for preparing inhibitor of skeletonema costatum (greville) cleve |
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| CNB2006100445886A CN100383055C (en) | 2006-05-26 | 2006-05-26 | Method for preparing inhibitor of skeletonema costatum (greville) cleve |
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| CN1850631A CN1850631A (en) | 2006-10-25 |
| CN100383055C true CN100383055C (en) | 2008-04-23 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101564050B (en) * | 2009-05-23 | 2013-06-19 | 中国船舶重工集团公司第七二五研究所 | Method for preparing Ulva pertusa Kjellm extract for marine antifoulant |
| CN103690571B (en) * | 2013-12-06 | 2016-04-13 | 淮海工学院 | From U. pertusa, phenolic acid and alkaloidal method and purposes are prepared in separation and purification |
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2006
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Non-Patent Citations (2)
| Title |
|---|
| 利用小麦秸控制赤潮生物生长的研究. 高洁等.海洋环境科学，第24卷第1期. 2005 * |
| 缘管浒苔对赤潮异弯藻的克生效应. 许妍.生态学报，第25卷第10期. 2005 * |
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