CN107318894B - The inhibitor and its preparation and application administered for microcystic aeruginosa - Google Patents
The inhibitor and its preparation and application administered for microcystic aeruginosa Download PDFInfo
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Abstract
The invention discloses a kind of inhibitor administered for microcystic aeruginosa, mainly include the raw material of following parts by weight: Chinese littleleaf box water extract 75-90, sodium nitrate 3-8, sodium carbonate 3-5 and flocculant 3-5, and the pH value of the inhibitor is 8.5.Its main component is obtained by extracting in the blade of Chinese littleleaf box, at low cost, quick, inhibits microcystic aeruginosa effect obvious, while not polluted-water, safety and environmental protection.Simultaneously, in the preparation method of inhibitor for microcystic aeruginosa improvement, it only needs that the blade of Chinese littleleaf box is dried, crushed and extracted, then it is mixed with other raw materials, preparation method is simply easy to grasp, and large scale quantities metaplasia is suitble to produce and use, in addition, by the way that a certain amount of traditional Chinese medicine ingredients are added in inhibitor, while can be improved inhibitory effect of the inhibitor to microcystic aeruginosa, moreover it is possible to realize the sterilization and purification to water quality.
Description
Technical field
The present invention relates to the preparation technical field of alga inhibitor, in particular to a kind of suppression administered for microcystic aeruginosa
Preparation and preparation method thereof and application method.
Background technique
In recent years, to improve humanistic environment, city taste is promoted, man-made lake becomes urban planning and developing primary choosing
It selects.But after building man-made lake, since mankind's activity makes water eutrophication, cause algalbloom, water quality deterioration occurs
" wawter bloom " does not have the effect of beautification life not only, has seriously affected people's lives instead.Wawter bloom algae such as microcystic aeruginosa
(Microcystis viridis), Hui Shi Microcystis aeruginosa (M.wesenbergii), anabena (Anabaena), nostoc
(Nostoc), the algae (Oscillatoria) etc. that quivers also can produce algae toxin, wherein Microcystin (MC) be a kind of distribution it is most wide,
The toxin of most serious is endangered, there is hepatotoxicity wind agitation, can lead to animal and the acute poisoning of people, Long Term Contact then can induced hepatocellular carcinoma, very
To death is caused, so Microcystis aeruginosa and its toxin are current main study subjects.As it can be seen that the eutrophication of city appearance water body
Tendency or the urgency of the existing research of potential trend, but it is necessary to property.
Algae-lysing microorganism is the general name for the quasi-microorganism for having inhibition or lethal effect to the algae in water body, in nature
Largely exist in environment, by direct or indirect mode, inhibits algal grown or kill algae, to dissolve frustule.Definition
By the microbial degradation process of research Microcystis aeruginosa, the changing rule of Microcystis aeruginosa and its toxin in water body can be not only grasped,
Water quality safety is correctly evaluated, improvement of the efficient degrading bacteria for micro-capsule algae pollution can also be isolated.Microorganism drop
Solution has many advantages, such as that at low cost, high safety, improvement period are short, is conducive to restoration of the ecosystem, and microbial degradation at present is considered as water
The important channel that Microcystis aeruginosa removes in body.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of inhibitor administered for microcystic aeruginosa, and main component is by Chinese littleleaf box
Blade in extract and obtain, it is at low cost, quick, inhibit microcystic aeruginosa effect obvious, while not polluted-water, safety collar
It protects.
It is a still further object of the present invention to provide a kind of preparation methods of inhibitor administered for microcystic aeruginosa, it is only necessary to
The blade of Chinese littleleaf box is dried, crushed and extracted, is then mixed with other raw materials, preparation method is simply easy to grasp, and is suitble to
Large scale quantities metaplasia is produced and is used.
It is a still further object of the present invention to provide a kind of inhibitor administered for microcystic aeruginosa, by inhibitor
A certain amount of traditional Chinese medicine ingredients are added, while can be improved inhibitory effect of the inhibitor to microcystic aeruginosa, moreover it is possible to real
Now to the sterilization of water quality and purification.
In order to realize these purposes and other advantages according to the present invention, provides and a kind of administer for microcystic aeruginosa
Inhibitor mainly includes the raw material of following parts by weight:
Chinese littleleaf box water extract 75-90, sodium nitrate 3-8, sodium carbonate 3-5 and flocculant 3-5, and the pH value of the inhibitor is
8.5。
It preferably, further include the auxiliary material of 20-30 parts by weight in the inhibitor administered for microcystic aeruginosa,
The auxiliary material is extracted to obtain by the raw material of following parts by weight:
Dandelion 25-35, viola mandshurica 15-25, lobelia chinensis 15-25, ajuga decumbens 10-20, azedarach 5-15, benefit are female
Careless 25-35, tuber of stemona 10-20, Herba Epimedii 15-25, fragrant thoroughwort 10-20, sweet wormwood 15-25, Kirilow Groundsel Herb 15-25, fruit of summer cypress 10-20 and
Asiatic plantain 15-25.
Preferably, in the inhibitor administered for microcystic aeruginosa, the flocculant is acrylamide, second two
One or more of four acetic acid disodium of amine, natural organic acids and acrylamide polymer.
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to
50-100 mesh obtains Chinese littleleaf box blade powder；
Step 2 takes Chinese littleleaf box blade powder 5-10 parts by weight to be added in the sterile distilled water of 500-1000 parts by weight, and stirring is equal
It is even and be heated to boiling 1h after, suction filtration take supernatant, obtain blade crude extract；
Step 3, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly by sediment
It filters out, obtains two grade blade crude extracts；
Step 4, the sodium nitrate and 0.08-0.3 parts by weight that 0.1-0.4 parts by weight are added in the two grade blades crude extract
Flocculant, stirring is completely dissolved to sodium nitrate and flocculant, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 5, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C save to get
The inhibitor administered for microcystic aeruginosa.
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to
50-100 mesh obtains Chinese littleleaf box blade powder；
Parts by weight are respectively dandelion 30, viola mandshurica 20, lobelia chinensis 20, ajuga decumbens 15, azedarach by step 2
10, the Chinese medicine of motherwort 30, the tuber of stemona 15, Herba Epimedii 20, fragrant thoroughwort 15, sweet wormwood 20, Kirilow Groundsel Herb 20, the fruit of summer cypress 15 and Asiatic plantain 20
It is crushed to 50-100 mesh after mixing, obtains auxiliary material powder；
Step 3 takes Chinese littleleaf box blade powder 5-10 parts by weight and auxiliary material powder 1-2 parts by weight that 500-1000 parts by weight are added
In sterile distilled water, after stirring evenly and being heated to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 4, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly by sediment
It filters out, obtains two grade blade crude extracts；
Step 5, the sodium nitrate and 0.08-0.3 parts by weight that 0.1-0.4 parts by weight are added in the two grade blades crude extract
Flocculant, stirring is completely dissolved to sodium nitrate and flocculant, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 6, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C save to get
The inhibitor administered for microcystic aeruginosa.
A kind of application method for the inhibitor administered for microcystic aeruginosa, is added microcystic aeruginosa algae for the inhibitor
In liquid, the consumption proportion of the inhibitor and microcystic aeruginosa algae solution is 1-5mL:1L.
Preferably, in the application method of the inhibitor administered for microcystic aeruginosa, the inhibitor is existed
Start to launch when Growth of Microcystis aeruginosa to logarithmic phase, injected volume 0.3-0.48mL/L, the dispensing period is 7d.
The present invention is include at least the following beneficial effects:
The present invention is by choosing frequently seen plants in 80 in lakeside, such as Chinese littleleaf box, wide yulan, mountain peach, acacia, to its blade into
Row extracts, and is added in microcystic aeruginosa algae solution under same culture conditions to its extracting solution, and at 0 day, 4 days and 7 days
To algae solution Determination of Chlorophyll-a, malonaldehyde and soluble protein content, these three important indicators for representing algae bio amount are divided
It does not measure, is compared and found by measurement result, wide yulan, dragon grab Chinese scholartree and Chinese littleleaf box leaf extract for copper in lakeside frequently seen plants
The growth of green Microcystis aeruginosa has certain inhibiting effect, and inhibiting rate is generally not more than 85%.Separately carry out experiment discovery inhibitor
There is influence to the inhibitory effect of inhibitor in the content of pH value, the content of sodium nitrate and calcium chloride, thus by adjusting suppression
The pH value of preparation, and after sodium nitrate and sodium carbonate is added in above-mentioned plant extraction liquid, it is carried out in microcystic aeruginosa algae solution real
It tests, after above-mentioned chemical agent is added, the inhibitory effect of inhibitor made of Chinese littleleaf box water extract is the most obvious for discovery, thus selects
The quantitative component of Chinese littleleaf box water extract, sodium nitrate and sodium carbonate is mixed and made into the inhibitor of microcystic aeruginosa, and inhibiting effect is obvious,
Inhibiting rate reached 98.9% at the 7th day.And Chinese littleleaf box is common green plant, so that using verdigris micro-capsule made of Chinese littleleaf box water extract
Algae inhibitor is low in cost, is suitable for that large-scale promotion produces and uses.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Detailed description of the invention
Fig. 1 is that inhibitor made of different plant extraction liquids is added to the measurement after Microcystis aeruginosa algae solution to chlorophyll-a content
Figure；
Fig. 2 is that inhibitor made of different plant extraction liquids is added to the measurement after Microcystis aeruginosa algae solution to mda content
Figure；
Fig. 3 is that inhibitor made of different plant extraction liquids is added to the survey after Microcystis aeruginosa algae solution to soluble protein content
Fixed figure；
Fig. 4 is the algicidal effect figure of the inhibitor of the present invention administered for microcystic aeruginosa at various ph values；
Fig. 5 is the algicidal effect figure of inhibitor of the present invention after adding sodium nitrate；
Fig. 6 is the histogram of influence of the calcium chloride to algicidal effect；
Fig. 7 is the comparison diagram of Examples 1 and 2 algicidal effect.
Specific embodiment
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art referring to specification text
Word can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
The present invention provides a kind of inhibitor administered for microcystic aeruginosa, mainly includes the raw material of following parts by weight: yellow
Poplar water extract 75-90, sodium nitrate 3-8, sodium carbonate 3-5 and flocculant 3-5, and the pH value of the inhibitor is 8.5.
In the above scheme, Chinese littleleaf box is not stringent to all requirements such as soil, moisture, easily survives, and in China, distribution is wide
It is general, there is resource abundant, the inhibitor using microcystic aeruginosa made of Chinese littleleaf box is at low cost, and inhibiting effect is obvious, to lake surface
Microcystic aeruginosa improvement have the effect of positive, by testing the pH value for finding inhibitor repeatedly at 8.5, algicidal effect is most
It is good, thus the pH value of the titration adjustment inhibitor of sodium hydroxide to 8.5 can be passed through in the preparation process of inhibitor.Sodium nitrate
Addition can reinforce algicidal effect.The addition of sodium carbonate can react with a small amount of calcium chloride contained in inhibitor, make
It obtains calcium ion and generates lime deposit combined with carbonate, and then remove the calcium ion in inhibitor, the presence meeting of calcium chloride
Algicidal effect is significantly affected, thus by the addition of sodium carbonate, by calcium chloride by removing in inhibitor, improve the inhibitor
To the inhibitory effect of microcystic aeruginosa.In addition, microcystic aeruginosa can be made to be killed simultaneously by the way that flocculant is added in inhibitor
The frustule of dissolution is coagulated together and precipitates, so that purpose of purifying the water quality is realized, so that lake surface or the water surface are limpid, effectively
Inhibit the generation of wawter bloom phenomenon.
It further include the auxiliary material of 20-30 parts by weight in one preferred embodiment, the auxiliary material is extracted by the raw material of following parts by weight
It obtains: dandelion 25-35, viola mandshurica 15-25, lobelia chinensis 15-25, ajuga decumbens 10-20, azedarach 5-15, motherwort
25-35, tuber of stemona 10-20, Herba Epimedii 15-25, fragrant thoroughwort 10-20, sweet wormwood 15-25, Kirilow Groundsel Herb 15-25, fruit of summer cypress 10-20 and vehicle
Preceding grass 15-25.
In the above scheme, by the way that the extracting solution of above-mentioned Chinese medicine is added in inhibitor, the inhibition of microcystic aeruginosa is made
With further enhancing, so that inhibiting rate has reached 99.6%, the complete inhibition to microcystic aeruginosa is realized substantially, and wherein Pu
Public English heat-clearing antibacterial has stronger inhibiting effect to gold-coloured staphylococci, hemolytic streptococcus and micrococcus catarrhalis, and viola mandshurica is clear
Heat has inhibiting effect to fungi, and lobelia chinensis has apparent inhibiting effect to fungi, and ajuga decumbens bactericidal effect is powerful, chinaberry
Sterilization and disinsection, the clearing heat and detoxicating sterilization loam of motherwort, the tuber of stemona kill the rich worm of roundworm cun worm, and the antifungal deinsectization of Herba Epimedii has suppression to mushroom
Production is used, and fragrant thoroughwort desinsection poison, sweet wormwood insecticidal and antibacterial, Kirilow Groundsel Herb can to invisible bacterium, it can be efficiently solved with sterilization and disinfestation
These problems, fruit of summer cypress sterilization dehumidifying, Asiatic plantain sterilization and disinfestation, thus described copper to be used for by being added to above-mentioned traditional Chinese medicine ingredients
In the inhibitor that green Microcystis aeruginosa is administered, the inhibiting rate of inhibitor can not only be improved, additionally it is possible to effectively prevent microcystic aeruginosa molten
Residue after algae breeds bacterium and pest, while also having the effect of sterilizing to lakebed bed mud and loam, in favor of in lake its
The growth of his water plant and the survival of fish.
In one preferred embodiment, the flocculant is acrylamide, Calcium Disodium Versenate, natural organic acids and propylene
One or more of amide polymer.
In the above scheme, it by the addition of flocculant, enables to algae residue to condense and sinks, so that on
The layer water surface is as clear as crystal, effectively prevents the generation of wawter bloom phenomenon.
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to
50-100 mesh obtains Chinese littleleaf box blade powder；
Step 2 takes Chinese littleleaf box blade powder 5-10 parts by weight to be added in the sterile distilled water of 500-1000 parts by weight, and stirring is equal
It is even and be heated to boiling 1h after, suction filtration take supernatant, obtain blade crude extract；
Step 3, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly by sediment
It filters out, obtains two grade blade crude extracts；
Step 4, the sodium nitrate and 0.08-0.3 parts by weight that 0.1-0.4 parts by weight are added in the two grade blades crude extract
Flocculant, stirring is completely dissolved to sodium nitrate and flocculant, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 5, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C save to get
The inhibitor administered for microcystic aeruginosa.
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to
50-100 mesh obtains Chinese littleleaf box blade powder；
Parts by weight are respectively dandelion 30, viola mandshurica 20, lobelia chinensis 20, ajuga decumbens 15, azedarach by step 2
10, the Chinese medicine of motherwort 30, the tuber of stemona 15, Herba Epimedii 20, fragrant thoroughwort 15, sweet wormwood 20, Kirilow Groundsel Herb 20, the fruit of summer cypress 15 and Asiatic plantain 20
It is crushed to 50-100 mesh after mixing, obtains auxiliary material powder；
Step 3 takes Chinese littleleaf box blade powder 5-10 parts by weight and auxiliary material powder 1-2 parts by weight that 500-1000 parts by weight are added
In sterile distilled water, after stirring evenly and being heated to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 4, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly by sediment
It filters out, obtains two grade blade crude extracts；
Step 5, the sodium nitrate and 0.08-0.3 parts by weight that 0.1-0.4 parts by weight are added in the two grade blades crude extract
Flocculant, stirring is completely dissolved to sodium nitrate and flocculant, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 6, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C save to get
The inhibitor administered for microcystic aeruginosa.
A kind of application method for the inhibitor administered for microcystic aeruginosa, is added microcystic aeruginosa algae for the inhibitor
In liquid, the consumption proportion of the inhibitor and microcystic aeruginosa algae solution is 1-5mL:1L.
In the above scheme, by the way that inhibitor is added in microcystic aeruginosa algae solution, find the dosage of inhibitor with algae
When liquid is 1-5mL:1L, the inhibitory effect of inhibitor is quite obvious, after 6mL inhibitor is added in 1L algae solution, and adds
When entering 5mL inhibitor, inhibitory effect simultaneously has no raising, thus limits the consumption proportion of inhibitor and microcystic aeruginosa algae solution as 1-
The dosage of 5mL:1L, i.e. inhibitor are few, and effect is obvious.It only needs for inhibitor to be added in algae solution, molten algae, application method can be realized
Simply it is easily achieved.
In one preferred embodiment, the inhibitor is started to launch in Growth of Microcystis aeruginosa to logarithmic phase, injected volume
For 0.3-0.48mL/L, the dispensing period is 7d.
In the above scheme, microcystic aeruginosa is grown rapidly in logarithmic phase, and inhibitor is added in logarithmic phase, can be most
The growth of the inhibition algae of big degree, and by the experiment of the inhibitor dosage in microcystic aeruginosa algae solution, discovery, which is worked as, to be faced
When large-scale lake surface, inhibitor is when the fountain height on lake surface is 0.3-0.48mL/L, and continuous sprinkling 7 days then can be to verdigris micro-capsule
Algae plays good algicidal effect, and overall inhibiting rate is up to 98.9%, and dosage is few, but effect is obvious.
Embodiment 1
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 7% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to 60
Mesh obtains Chinese littleleaf box blade powder；
Step 2 takes 10 parts by weight of Chinese littleleaf box blade powder to be added in the sterile distilled water of 1000 parts by weight, stirs evenly and adds
After heat to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 3, the sodium carbonate that 0.2 parts by weight are added in the blade crude extract, after mixing evenly filter out sediment,
Obtain two grade blade crude extracts；
The flocculant of step 4, the sodium nitrate that 0.3 parts by weight are added in the two grade blades crude extract and 0.2 parts by weight,
Stirring to sodium nitrate and flocculant is completely dissolved, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 5, by the three grade blades crude extract through 121 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C and save to get described
The inhibitor administered for microcystic aeruginosa.
It is put into 1L microcystic aeruginosa algae solution and is cultivated by the inhibitor 5mL for preparing embodiment 1, in culture to the
At 7 days, the content of algae solution Determination of Chlorophyll-a is measured, the suppression of inhibitor prepared by discovery embodiment 1 to microcystic aeruginosa
Rate processed has reached 98.9%.
Embodiment 2
A kind of preparation method for the inhibitor administered for microcystic aeruginosa, mainly comprises the steps that
It is 7% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to 60
Mesh obtains Chinese littleleaf box blade powder；
Parts by weight are respectively dandelion 30, viola mandshurica 20, lobelia chinensis 20, ajuga decumbens 15, azedarach by step 2
10, the Chinese medicine of motherwort 30, the tuber of stemona 15, Herba Epimedii 20, fragrant thoroughwort 15, sweet wormwood 20, Kirilow Groundsel Herb 20, the fruit of summer cypress 15 and Asiatic plantain 20
It is crushed to 70 mesh after mixing, obtains auxiliary material powder；
Step 3 takes 10 parts by weight of Chinese littleleaf box blade powder and 1.5 parts by weight of auxiliary material powder that the sterile steaming of 1000 parts by weight is added
In distilled water, after stirring evenly and being heated to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 4, the sodium carbonate that 0.2 parts by weight are added in the blade crude extract, after mixing evenly filter out sediment,
Obtain two grade blade crude extracts；
The flocculant of step 5, the sodium nitrate that 0.3 parts by weight are added in the two grade blades crude extract and 0.2 parts by weight,
Stirring to sodium nitrate and flocculant is completely dissolved, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 6, by the three grade blades crude extract through 121 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C and save to get described
The inhibitor administered for microcystic aeruginosa.
1L microcystic aeruginosa algae solution (algae solution condition of culture and embodiment are put by the inhibitor 5mL for preparing embodiment 2
Algae solution used in 1 is identical) in cultivated, when culture was to the 7th day, the content of algae solution Determination of Chlorophyll-a is measured,
It was found that inhibitor prepared by embodiment 2 has reached 99.6% to the inhibiting rate of microcystic aeruginosa.
Experimental data
1, by being extracted to 80 kinds of lakeside frequently seen plants, therefrom select Chinese littleleaf box, wide yulan, dragon grab Chinese scholartree, mountain peach and
Chinese littleleaf box water extract, wide yulan water extract, dragon are grabbed Huai Shui and mentioned by five kinds of the acacia plants inhibited for microcystic aeruginosa
Inhibitor made of object, mountain peach water extract and acacia water extract and equivalent sterile distilled water is separately added into the identical culture item of equivalent
In the microcystic aeruginosa algae solution cultivated under part, and to the content of chlorophyll-a therein, malonaldehyde and soluble protein in culture 0
It, 4 days and 7 days whens measure, the comparing result obtained is shown in Fig. 1-3, by the result of Fig. 1-3 as it can be seen that wide yulan water extract pair
The most obvious in the inhibitory effect of microcystic aeruginosa, dragon grabs Chinese scholartree and Chinese littleleaf box effect is slightly worse, thus chooses wide yulan, Chinese pagoda tree and Huang
The raw material for preparing microcystic aeruginosa inhibitor of three kinds of poplar alternately.
2, it grabs in inhibitor made of Chinese scholartree water extract Chinese littleleaf box water extract, wide yulan water extract and dragon and passes through hydrogen-oxygen respectively
The titration of change sodium is respectively 6.5,7.5 and 8.5 with the pH value for adjusting the inhibitor, is then added separately to the inhibitor
In the microcystic aeruginosa algae solution cultivated under the same culture conditions of equivalent, and the content of chlorophyll-a therein is measured,
Measurement result is as shown in figure 4, algicidal effect of the pH value at 8.5 is more preferable.
3, it grabs Chinese littleleaf box water extract, wide yulan water extract and dragon and passes through addition in inhibitor made of Chinese scholartree water extract respectively
The inhibitor is then added separately to the microcystic aeruginosa algae cultivated under the same culture conditions of equivalent by not same amount sodium nitrate
In liquid, and the content of chlorophyll-a therein is measured, measurement result is as shown in figure 5, when more sodium nitrate additive amount
Algicidal effect is more preferable, and the additive amount of sodium nitrate is 3-8 parts by weight at this time.
4, it grabs Chinese littleleaf box water extract, wide yulan water extract and dragon and passes through addition in inhibitor made of Chinese scholartree water extract respectively
The inhibitor is then added separately to the microcystic aeruginosa algae cultivated under the same culture conditions of equivalent by not same amount calcium chloride
In liquid, and the content of chlorophyll-a therein is measured, measurement result as shown in fig. 6, when fewer content of calcium chloride it is molten
Algae effect is more preferable, thus addition sodium carbonate 3-5 parts by weight can remove the calcium chloride in inhibitor substantially.
5, that inhibitor made of Examples 1 and 2 is added separately to the verdigris cultivated under the same culture conditions of equivalent is micro-
In capsule algae algae solution, and the content of chlorophyll-a therein is measured, measurement result is as shown in fig. 7, the molten algae of embodiment 2 is imitated
Fruit is substantially better than embodiment 1, and the inhibiting rate of embodiment 2 is implemented substantially close to 100% in addition, passing through observation under the microscope
Water body after the molten algae of example 1 and 2 finds that bacterial content is minimum in the water body of embodiment 2, and water quality is substantially better than embodiment 1.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and legend shown and described herein.
Claims (6)
1. a kind of inhibitor administered for microcystic aeruginosa, wherein mainly include the raw material of following parts by weight:
Chinese littleleaf box water extract 75-90, sodium nitrate 3-8, sodium carbonate 3-5 and flocculant 3-5, and the pH value of the inhibitor is 8.5；
Wherein, the preparation method of the inhibitor administered for microcystic aeruginosa mainly comprises the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to 50-
100 mesh obtain Chinese littleleaf box blade powder；
Step 2 takes Chinese littleleaf box blade powder 5-10 parts by weight to be added in the sterile distilled water of 500-1000 parts by weight, stirs evenly simultaneously
After being heated to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 3, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly filter sediment
It removes, obtains two grade blade crude extracts；
The wadding of step 4, the sodium nitrate that 0.1-0.4 parts by weight are added in the two grade blades crude extract and 0.08-0.3 parts by weight
Solidifying agent, stirring to sodium nitrate and flocculant are completely dissolved, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 5, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C and save to get described
The inhibitor administered for microcystic aeruginosa.
2. the inhibitor administered as described in claim 1 for microcystic aeruginosa, wherein further include the auxiliary of 20-30 parts by weight
Material, the auxiliary material are extracted to obtain by the raw material of following parts by weight:
Dandelion 25-35, viola mandshurica 15-25, lobelia chinensis 15-25, ajuga decumbens 10-20, azedarach 5-15, motherwort
25-35, tuber of stemona 10-20, Herba Epimedii 15-25, fragrant thoroughwort 10-20, sweet wormwood 15-25, Kirilow Groundsel Herb 15-25, fruit of summer cypress 10-20 and vehicle
Preceding grass 15-25.
3. as described in claim 1 for microcystic aeruginosa administer inhibitor, wherein the flocculant be acrylamide,
One or more of Calcium Disodium Versenate, natural organic acids and acrylamide polymer.
4. the inhibitor administered as described in claim 1 for microcystic aeruginosa, wherein described to be administered for microcystic aeruginosa
The preparation method of inhibitor mainly comprise the steps that
It is 5-8% that step 1, the blade for winning Chinese littleleaf box, which are dried to water content after cleaning, and the blade after drying is crushed to 50-
100 mesh obtain Chinese littleleaf box blade powder；
Parts by weight are respectively dandelion 30, viola mandshurica 20, lobelia chinensis 20, ajuga decumbens 15, azedarach 10, benefit by step 2
The Chinese medicine mixing of Brittle Falsepimpernel Herb 30, the tuber of stemona 15, Herba Epimedii 20, fragrant thoroughwort 15, sweet wormwood 20, Kirilow Groundsel Herb 20, the fruit of summer cypress 15 and Asiatic plantain 20
After be crushed to 50-100 mesh, obtain auxiliary material powder；
Step 3 takes Chinese littleleaf box blade powder 5-10 parts by weight and auxiliary material powder 1-2 parts by weight that the sterile of 500-1000 parts by weight is added
In distilled water, after stirring evenly and being heated to boiling 1h, suction filtration takes supernatant, obtains blade crude extract；
Step 4, the sodium carbonate that 0.08-0.3 parts by weight are added in the blade crude extract, after mixing evenly filter sediment
It removes, obtains two grade blade crude extracts；
The wadding of step 5, the sodium nitrate that 0.1-0.4 parts by weight are added in the two grade blades crude extract and 0.08-0.3 parts by weight
Solidifying agent, stirring to sodium nitrate and flocculant are completely dissolved, and adjusting pH value is 8.5, obtains three grade blade crude extracts；
Step 6, by the three grade blades crude extract through 115-125 DEG C of high-temperature sterilization 20min, be cooled to 4 DEG C and save to get described
The inhibitor administered for microcystic aeruginosa.
5. a kind of application method for the inhibitor administered as described in claim 1 for microcystic aeruginosa, wherein by the suppression
Preparation is added in microcystic aeruginosa algae solution, and the consumption proportion of the inhibitor and microcystic aeruginosa algae solution is 1-5mL:1L.
6. the application method for the inhibitor administered as claimed in claim 5 for microcystic aeruginosa, wherein by the inhibitor
Start to launch in Growth of Microcystis aeruginosa to logarithmic phase, injected volume 0.3-0.48mL/L, the dispensing period is 7d.
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| CN103461390A (en) * | 2013-09-11 | 2013-12-25 | 安徽师范大学 | Inhibition method of microcystis aeruginosa |
| CN104261523A (en) * | 2014-10-10 | 2015-01-07 | 安徽师范大学 | Method for restraining microcystis aeruginosa by using bengal waterdropwort herb |
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| CN103461390A (en) * | 2013-09-11 | 2013-12-25 | 安徽师范大学 | Inhibition method of microcystis aeruginosa |
| CN104261523A (en) * | 2014-10-10 | 2015-01-07 | 安徽师范大学 | Method for restraining microcystis aeruginosa by using bengal waterdropwort herb |
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| 广玉兰、龙爪槐和黄杨化感物质对铜绿微囊藻生长的抑制;周晓见等;《环境污染与防治》;20101130;第32卷(第11期);第34-38页 * |
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