CN1961681A - Preparation method and use of a fish toxic made of tea saponin - Google Patents
Preparation method and use of a fish toxic made of tea saponin Download PDFInfo
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- CN1961681A CN1961681A CN 200610135288 CN200610135288A CN1961681A CN 1961681 A CN1961681 A CN 1961681A CN 200610135288 CN200610135288 CN 200610135288 CN 200610135288 A CN200610135288 A CN 200610135288A CN 1961681 A CN1961681 A CN 1961681A
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Abstract
The invention relates to a method for preparing tea saponin toxic fish agent, wherein said methodn comprises that it uses tea slag as material, uses hydrophilic solvent solution to extract 1-3times under 50-80Deg. C, while each time costs 1-3hours; combining the extractives; depressurizing the filter at -0.06-0.095MPa under 50-80Deg. C, to recycle the solvent, concentrating in vacuum into concentrated liquid at 12-15degree (60Deg. C), adding common salt at 0.8% and calcium hydrate at 0.5%, to prepare the agent; and it can be atomized and dried into solid. The inventive agent can effectively clean the foreign fish. The invention has simple process and low cost.
Description
Technical field
The present invention relates to a kind of cleaning chemistry production method of from plant, extracting medicinal active ingredient, more specifically relate to a kind of method and purposes that natural tea saponin active component prepares environmentally friendly fish toxic made of tea saponin of from tea dregs of rice discarded object, extracting.
Background technology
In the aquaculture process, killing wild fishes is a critical stage that must experience, when killing trash fish, normal because of the difference of the difference of breed variety, multiple disease and the facility of medicament sources, and use different types of fish toxic, use the logical fish toxic that kills type as breeding spand is normal, and the shrimp crab is cultured the normal fish toxic that uses choosing to kill type in the pool.
Effective removing of wild fishes is a serious hindrance of puzzlement shrimp crab aquaculture development.In the aquaculture of current shrimp crab, as the river crab of freshwater aquiculture, eriocheir sinensis, freshwater shrimp, grass shrimp, Macrobrachium rosenbergii etc., the prawn of mariculture, Penaeus Vannmei, crab etc., often have some wild fisheses to be mixed in wherein, wild fishes can be fought for bait with the shrimp crab, nibbles the shrimp crab young, disturb shrimp crab shell growth.Therefore, raiser's palpus use medicine comes the wild fishes in the selective clearing shrimp crab breed pool, the modal organo-chlorine pesticide penta sodium pentachlorophenate that is to use.Because penta sodium pentachlorophenate is to the high poison of people and animals and to the severe contamination of environment, in April, 2002, the Ministry of Agriculture's 193 commands just clearly banned use of.The raiser urgently wishes have a kind of energy to substitute the medicine of penta sodium pentachlorophenate, in the wild fishes in effectively removing the shrimp crab breed pool, can avoid fish toxic prawn crab to cause and poison and the underproduction.
Tea Saponin just possesses this selectivity and kills the function that the shrimp crab is cultured the wild fishes in the pool.An amount of Tea Saponin prawn crab does not only have toxicity, helps shell and the volume increase of shrimp crab on the contrary.In natural environment, Tea Saponin can be degraded by microorganisms, and its malicious fish activity complete obiteration after 3～5 days is expected to be developed to the friendly type aquaculture of a kind of ideal environment fish toxic.
Tea Saponin is the Pentacyclic triterpene saponins compounds that extracts from the tea dregs of rice, it is a kind of natural nonionic surface active agent, have biologically actives such as certain malicious fish, antibiotic, antiviral, desinsection, anti-inflammatory, reducing blood lipid and antifertility, be widely used in industries such as chemical industry, medicine, agricultural chemicals, aquatic products and food.Tea Saponin is 50～150mg/Kg body weight every day to the safe intake of warm blooded animal, and is nontoxic when people and animals are oral.
Oil tea is the important woody oleiferous plants crop of China, is distributed in the knob that economize in Fujian, Jiangxi, Hunan, Guizhou, Guangxi, Zhejiang, Anhui, Guangdong etc. more, accounts for more than 70% of national woody edible oil materials area, and cultivation history reaches more than 2000 year.At present, the whole nation can be produced 500000 tons in tea dregs of rice waste material per year, owing to contain 5～7% Residual oil and 6～10% Tea Saponin in the tea dregs of rice, can not directly be used as animal feed or fertilizer.
Because Tea Saponin is soluble in the hydrophilic solvents such as hot water and methyl alcohol, ethanol, acetone, therefore, can adopt the mineral salt acidity or the hydrophilic solvents such as alkaline aqueous solution or methyl alcohol, acetone of hot water, pH value 2～12 to extract.
Summary of the invention
The object of the present invention is to provide a kind of Preparation method and use of fish toxic made of tea saponin, this preparation method has that technology is simple, extraction efficiency is high, the characteristics that production cost is low, the fish toxic made of tea saponin of this preparation method's preparation has the purposes that selectivity is killed wild fishes in the aquaculture of shrimp crab.
The preparation method of a kind of fish toxic made of tea saponin of the present invention is: with the tea dregs of rice is raw material, adopt 25～75% hydrophilic solvent aqueous solution of 5～15 times of tea dregs of rice dry weights (W/W), under 50～80 ℃ of conditions, extract 1～3 time, each extraction time is 1～3 hour, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃) through decompression and solvent recovery, vacuum concentration, add the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), make the liquid dosage form fish toxic made of tea saponin.
Fish toxic made of tea saponin of the present invention has the purposes that selectivity is killed wild fishes in the aquaculture of shrimp crab.
The present invention has following remarkable advantage:
1) than existing preparation method, the present invention adopts the hydrophilic solvent aqueous solution such as methyl alcohol, acetone, extract Tea Saponin medicinal active ingredient in the tea dregs of rice, make fish toxic made of tea saponin, its advantage is: the solubility property of hydrophilic solvents such as methyl alcohol, acetone is good, penetration capacity to the tea dregs of rice is strong, and easier being penetrated in the tea dregs of rice internal organizational structure improved the dissolution rate and the extraction efficiency of Tea Saponin composition in the tea dregs of rice greatly.
2) preferentially select for use the advantage of methyl alcohol or acetone to be: the extraction effect of hydrophilic solvents such as the mineral salt acidity of methyl alcohol, ethanol, propyl alcohol, isopropyl alcohol, acetone or pH value 2～12 or alkaline aqueous solution is suitable, but for other hydrophilic solvent, the boiling point of methyl alcohol or acetone is lower, energy consumption is littler, can significantly reduce the preparation cost of product.
3) the present invention extracts in the tea dregs of rice Tea Saponin composition and makes fish toxic, have efficient, the low-residual of modern biopesticide product, to people and animals and shrimp crab low toxicity, environment amenable advantage, can substitute the high poison of people and animals, serious organophosphorous pesticide and the organo-chlorine pesticide of environmental pollution, can adapt to and satisfy the medication demand of modern aquaculture, meet comprehensive utilization agriculture and forestry organic waste material that country advocates, promote the recycling economy technology, ensure the industrial policy of aquatic products and food security.
Embodiment
With the tea dregs of rice is raw material, adopt 25～75% hydrophilic solvent aqueous solution of 5～15 times of tea dregs of rice dry weights (W/W), under 50～80 ℃ of conditions, extract 1～3 time, each extraction time is 1～3 hour, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃) through decompression and solvent recovery, vacuum concentration, the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.
The atomized drying condition is: feeding liquid concentration reaches 13～16 Baume degrees (60 ℃), 160～250 ℃ of PG-5 type spray dryer inlet temperatures, 60～110 ℃ of outlet temperatures, centrifugal head operating pressure 1.6～3.0kgf/cm
2
Above-mentioned hydrophilic solvent can be one or more mixed solvents in methyl alcohol, acetone, ethanol, propyl alcohol or the isopropyl alcohol, preferentially selects methyl alcohol or acetone for use, and hydrophilic solvent also can be the mineral salt acidity or the alkaline aqueous solution of pH value 2～12.
Tea Saponin content adopts vanillic aldehyde-concentrated sulfuric acid colorimetric method for determining.Get the sample liquid 1ml that dilutes suitable multiple, add the 0.5ml vanillic aldehyde, add the 2.5ml concentrated sulfuric acid again, behind the reaction 10min, measure its absorbance down in the 580nm wavelength.With reagent is blank, is standard control with ginsenoside Re.After measured, the used middle Tea Saponin content of the Fujian product tea dregs of rice (water content 4.3%) of experiment is 7.6～8.9%.
Preparation method's of the present invention embodiment is presented below:
Embodiment 1
The tea dregs of rice powder 2.3kg that crosses 16 mesh sieves is put into extractor, methyl alcohol-acetone (2: 1) aqueous solution of 50% with 10 times of tea dregs of rice powder dry weights (W/W), refluxing extraction is 3 times under 70 ℃ of conditions, each 2 hours, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃) through decompression and solvent recovery, vacuum concentration, the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 93.2% in the tea dregs of rice, and Tea Saponin content reaches 72.0% in the liquid dosage form fish toxic.
Embodiment 2
The tea dregs of rice powder 2.6kg that crosses 16 mesh sieves is put into extractor, 25% methanol aqueous solution with 15 times of tea dregs of rice powder dry weights (W/W), under 50 ℃ of conditions, extract 2 times, each 3 hours, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 93.1% in the tea dregs of rice, and Tea Saponin content reaches 69.7% in the liquid dosage form fish toxic.
Embodiment 3
The tea dregs of rice powder 3.1kg that crosses 16 mesh sieves is put into extractor, 75% methanol aqueous solution with 5 times of tea dregs of rice powder dry weights (W/W), refluxing extraction is 1 hour under 80 ℃ of conditions, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 95.1% in the tea dregs of rice, and Tea Saponin content reaches 65.3% in the liquid dosage form fish toxic.
Embodiment 4
The tea dregs of rice powder 3.0kg that crosses 16 mesh sieves is put into extractor, 75% ethanol water with 5 times of tea dregs of rice powder dry weights (W/W), under 80 ℃ of conditions, extracted 1 hour, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 93.6% in the tea dregs of rice, and Tea Saponin content reaches 70.9% in the liquid dosage form fish toxic.
Embodiment 5
The tea dregs of rice powder 3.3kg that crosses 16 mesh sieves is put into extractor, 45% methyl alcohol-ethanol (3: 1) aqueous solution with 8 times of tea dregs of rice powder dry weights (W/W), under 56 ℃ of conditions, extract 2 times, each 2 hours, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃) through decompression and solvent recovery, vacuum concentration, the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 94.5% in the tea dregs of rice, and Tea Saponin content reaches 69.2% in the liquid dosage form fish toxic.
Embodiment 6
The tea dregs of rice powder 2.6kg that crosses 16 mesh sieves is put into extractor, 48% ethanol water with 15 times of tea dregs of rice powder dry weights (W/W), under 50 ℃ of conditions, extract 3 times, each 3 hours, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 92.8% in the tea dregs of rice, and Tea Saponin content reaches 67.0% in the liquid dosage form fish toxic.
Embodiment 7
The tea dregs of rice powder 2.5kg that crosses 16 mesh sieves is put into extractor, 25% aqueous acetone solution with 15 times of tea dregs of rice powder dry weights (W/W), under 50 ℃ of conditions, extract 3 times, each 3 hours, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 92.0% in the tea dregs of rice, and Tea Saponin content reaches 68.9% in the liquid dosage form fish toxic.
Embodiment 8
The tea dregs of rice powder 3.2kg that crosses 16 mesh sieves is put into extractor, 75% aqueous acetone solution with 5 times of tea dregs of rice powder dry weights (W/W), refluxing extraction is 2 times under 80 ℃ of conditions, each 1 hour, merge extract, filter, filtrate-0.06～-0.095MPa, 50～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃), the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form through decompression and solvent recovery, vacuum concentration; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 91.6% in the tea dregs of rice, and Tea Saponin content reaches 64.3% in the liquid dosage form fish toxic.
Embodiment 9
The tea dregs of rice powder 2.5kg that crosses 16 mesh sieves is put into extractor, sodium hydrate aqueous solution with the pH9.2 of 15 times of tea dregs of rice powder dry weights (W/W), under 80 ℃ of conditions, extract 3 times, each 2 hours, filter, filtrate-0.06～-0.095MPa, 65～80 ℃ of conditions under, become the concentrate of 12～15 Baume degrees (60 ℃) through decompression and solvent recovery, vacuum concentration, the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), the fish toxic made of tea saponin of canned one-tenth liquid dosage form; Also can further add medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.After measured, the recovery rate of Tea Saponin is 89.0% in the tea dregs of rice, and Tea Saponin content reaches 61.2% in the liquid dosage form fish toxic.
Explanation about fish toxic made of tea saponin purposes of the present invention
Fish toxic made of tea saponin to previous embodiment 1 preparation carries out the pharmacodynamics evaluation experimental.
1. material
Crucian 6～8cm, grass carp 5～7cm, pseudorasbora parva 2～3cm, white hairtail 6～10cm, Culter fish 6～9cm, loach 15～20cm, river crab 25～40g, Penaeus Vannmei 3～4cm is available from end of the bridge aquatic products market, flood mountain, Fuzhou City, Fujian Province.Culture water tank (70cm * 55cm * 40cm), fish toxic made of tea saponin (Tea Saponin content 72.0%), University of Fuzhou's self-control product.
2. method
The depth of water 30cm of water tank culture experiment, 25 ℃ of water temperatures and pH value 6.8, the inflation of employing inflator pump, experiment fishes and shrimps aeration in the running water that removes chlorine is supported 2d temporarily, each water tank is put each 20 of crucian, grass carp, carp, pseudorasbora parva, Culter fish, white hairtail, loach, swamp eel, river crab or Penaeus Vannmeis respectively in a suitable place to breed, fish toxic is offerd medicine with different concentration, investigate its toxicity, adopt the Return Law to calculate the 24h half lethal dose of fish toxic fish shrimp crab.Each experiment repeats 6 times at least.
3. functional evaluation result
3.1 fish toxic made of tea saponin is to the acute toxicity effect of common fish
In 24h, when dispensing concentration≤1.0mg/L, fish toxic does not all have the poisoning ability to each experiment with fish; Along with the increase of dispensing concentration, fish toxic rises gradually to the poisoning ability of fish, when concentration 〉=2.5mg/L, just fish all can be killed in 24h.But it is dead that the fish that survive in 24h are difficult in follow-up experimental period, and in 24h, fish toxic made of tea saponin is to the LD of common fish
50Be 1.60～1.75mg/L.
3.2 fish toxic made of tea saponin is to the active continuous capability of the ichthyotoxin of fish
The experiment water temperature changes 28 ℃, dispensing concentration 2.0mg/L into, all puts 18 tail grass carps in a suitable place to breed respectively in each dispensing back aeration is handled the water tank of 0h, 12h, 24h, 48h, 72h and 96h, observes the 48h survival rate, investigates fish toxic to the active continuous capability of the ichthyotoxin of grass carp.The result is as seen, and is all dead the 18 tail grass carps that 0h and 12h put in a suitable place to breed, the grass carp that 24h puts in a suitable place to breed 3 tails of surviving, and the grass carp that 48h puts in a suitable place to breed later on all survives.The ichthyotoxin activity that fish toxic made of tea saponin is described can comparatively fast be degraded in the aeration environment, has the friendly characteristic of fine environment, can be used for removing in the aquaculture predator fish.
3.3 fish toxic made of tea saponin is to the poisoning ability of loach
Loach is stronger to the tolerance of fish toxic, and when dispensing concentration≤2.0mg/L, the loach survival rate is 100% in 0～48h.The fish toxic of variable concentrations presents good dosage-medicine efficacy relation to the poisoning ability of loach, along with the increase of dispensing concentration, fish toxic improves gradually to the poisoning ability of loach, when dispensing concentration 〉=4.5mg/L, in 24h, just loach all can be killed its LD
50Be 3.0mg/L, be higher than the LD of common fish
50
3.4 fish toxic made of tea saponin is to the poisoning ability of Penaeus Vannmei and river crab
The depth of water that only changes experiment is 7.5cm, and dispensing concentration is respectively 5,10,15,20,30,40,50mg/L, investigates the poisoning ability of fish toxic made of tea saponin to Penaeus Vannmei and river crab.The result shows in the dispensing concentration range of experiment, to have only Penaeus Vannmei dead 2 tails when 40mg/L of one batch, and the Penaeus Vannmei survival rate of all the other batches all reaches 100%; In each 50mg/L experiment, river crab 48h survival rate only reaches 90%, does not see death in all the other batches.Illustrate that Penaeus Vannmei, river crab have higher tolerance to Tea Saponin.
4 conclusions
Water tank culture experiment result shows that fish toxic made of tea saponin has stronger toxicity to common freshwater fish, and presents good dosage-drug effect and time-medicine efficacy relation.When dispensing concentration 2.5mg/L, said preparation can all kill LD with experiment with fish in 24h
50Be 1.60～1.75mg/L, the LD of loach
50Be 3.0mg/L; When dispensing concentration reached 50mg/L, Penaeus Vannmei 48h survival rate can reach 100%, and river crab 48h survival rate can reach more than 90%.Result of study shows, fish toxic made of tea saponin has significant poisoning ability to common fish, but it is very safe to Penaeus Vannmei and river crab, can be developed further into a kind of efficient, low-residual, to people and animals and shrimp crab low toxicity, environment amenable plant class fish toxic, be used for effective removing of shrimp crab aquaculture wild fishes.
Above embodiment is intended to further describe for example the present invention, rather than limits the present invention by any way.
The present invention is novel, and technology is simple, the extraction efficiency height, and production cost is low, has bigger generalization.
Claims (7)
1. the preparation method of a fish toxic made of tea saponin, it is characterized in that: with the tea dregs of rice is raw material, adopt 25～75% hydrophilic solvent aqueous solution of 5～15 times of tea dregs of rice dry weights (W/W), under 50～80 ℃ of conditions, extract 1～3 time, each extraction time is 1～3 hour, merge extract, filter, filtrate-0.06～-0.095MPa, under 50～80 ℃ of conditions, through decompression and solvent recovery, vacuum concentration becomes the concentrate of 12～15 Baume degrees (60 ℃), add the sodium chloride of concentrate weight 0.8% and 0.5% slaked lime (W/W), make the fish toxic made of tea saponin of liquid dosage form.
2. the preparation method of a kind of fish toxic made of tea saponin according to claim 1, it is characterized in that: the fish toxic made of tea saponin of described liquid dosage form further adds medicinal dextrin, make concentrate reach 13～16 Baume degrees (60 ℃), be spray dried to the fish toxic made of tea saponin of solid dosage forms.
3. the preparation method of a kind of fish toxic made of tea saponin according to claim 2, it is characterized in that: described atomized drying condition is: feeding liquid concentration reaches 13～16 Baume degrees (60 ℃), 160～250 ℃ of PG-5 type spray dryer inlet temperatures, 60～110 ℃ of outlet temperatures, centrifugal head operating pressure 1.6～3.0kgf/cm
2
4. the preparation method of fish toxic made of tea saponin according to claim 1, it is characterized in that: described hydrophilic solvent is one or more mixed solvents in methyl alcohol, acetone, ethanol, propyl alcohol or the isopropyl alcohol.
5. the preparation method of fish toxic made of tea saponin according to claim 1, it is characterized in that: described hydrophilic solvent is the mineral salt acidity or the alkaline aqueous solution of pH value 2～12.
6. the preparation method of a kind of fish toxic made of tea saponin according to claim 1 is characterized in that: the described tea dregs of rice derive from one or more in the tea cake waste material of fruit, tea seed, tea shell and process thereof of plant of theaceae oil tea.
7. the purposes of a fish toxic made of tea saponin as claimed in claim 1 or 2, it is characterized in that: described fish toxic made of tea saponin has the purposes that selectivity is killed wild fishes in the aquaculture of shrimp crab.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102948418A (en) * | 2011-08-26 | 2013-03-06 | 浙江海洋学院 | Tea saponin tablet and preparation method thereof |
| CN103073599A (en) * | 2013-01-18 | 2013-05-01 | 北京林业大学 | Concentrating and extracting method for saponins |
| CN108432809A (en) * | 2018-03-30 | 2018-08-24 | 湖南淳湘农林科技有限公司 | A kind of cleaner for the cultivation of shrimp crab |
| CN110463635A (en) * | 2019-08-19 | 2019-11-19 | 中国水产科学研究院黄海水产研究所 | Tea Saponin is being used to prepare the application reduced in the Crab larvae death rate and promotion Crab larvae husking preparation |
| CN113142248A (en) * | 2021-04-23 | 2021-07-23 | 浙江久晟油茶科技股份有限公司 | Pond cleaning agent for aquaculture and preparation method thereof |
| CN113575618A (en) * | 2021-09-03 | 2021-11-02 | 滁州学院 | Shrimp and crab culture trash fish scavenging agent and preparation method thereof |
| CN114097789A (en) * | 2021-12-01 | 2022-03-01 | 湖南省林业科学院 | Method for preparing compound tea saponin pond cleaning agent by taking tea seed meal as raw material |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191069A (en) * | 1997-02-21 | 1998-08-26 | 中国科学院化学研究所 | Sasangua-seed-saponin and its use |
| CN1109042C (en) * | 1999-08-17 | 2003-05-21 | 湖南省林业科学院 | Process for extracting theasaponin from tea cake by using n-butyl alcohol |
| CN100522982C (en) * | 2004-10-01 | 2009-08-05 | 南华大学 | Production process for extracting tea saponin from tea-oil tree cake by using water as dissoluent |
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2006
- 2006-12-06 CN CNB2006101352889A patent/CN100431424C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102948418A (en) * | 2011-08-26 | 2013-03-06 | 浙江海洋学院 | Tea saponin tablet and preparation method thereof |
| CN103073599A (en) * | 2013-01-18 | 2013-05-01 | 北京林业大学 | Concentrating and extracting method for saponins |
| CN103073599B (en) * | 2013-01-18 | 2015-01-28 | 北京林业大学 | Concentrating and extracting method for saponins |
| CN108432809A (en) * | 2018-03-30 | 2018-08-24 | 湖南淳湘农林科技有限公司 | A kind of cleaner for the cultivation of shrimp crab |
| CN110463635A (en) * | 2019-08-19 | 2019-11-19 | 中国水产科学研究院黄海水产研究所 | Tea Saponin is being used to prepare the application reduced in the Crab larvae death rate and promotion Crab larvae husking preparation |
| CN113142248A (en) * | 2021-04-23 | 2021-07-23 | 浙江久晟油茶科技股份有限公司 | Pond cleaning agent for aquaculture and preparation method thereof |
| CN113575618A (en) * | 2021-09-03 | 2021-11-02 | 滁州学院 | Shrimp and crab culture trash fish scavenging agent and preparation method thereof |
| CN114097789A (en) * | 2021-12-01 | 2022-03-01 | 湖南省林业科学院 | Method for preparing compound tea saponin pond cleaning agent by taking tea seed meal as raw material |
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