CN1191069A - Sasangua-seed-saponin and its use - Google Patents
Sasangua-seed-saponin and its use Download PDFInfo
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- CN1191069A CN1191069A CN97100066A CN97100066A CN1191069A CN 1191069 A CN1191069 A CN 1191069A CN 97100066 A CN97100066 A CN 97100066A CN 97100066 A CN97100066 A CN 97100066A CN 1191069 A CN1191069 A CN 1191069A
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- seed
- sasangua
- saponin
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- ots
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Abstract
A sasangua-seed-saponin is a mixture of 5 teasaponins with respective melting point of 172, 190, 204, 218, or 228 deg.C, which has been proved by infrared, ultraviolet, thermoanalytic and high-pressure liquid chromatographic tests, and can be used to kill harmful fish in prawn culturing pond and fly and inhibit the growth of fly larva.
Description
The present invention relates to a kind of Tea Saponin, particularly a kind of Sasangua-seed-saponin (OTS) and uses thereof.
" daily chemical industry " second phase in 1987, P32 has reported a kind of Tea Saponin as natural surfactant, and its fusing point is 224 ℃.CN 89105679.3 discloses the insecticide " the gram mite is clean " " killing a red-spotted lizard spirit " that extracts Tea Saponin and make from cake of camellia oleifera seeds.Its animal acute toxicity test result is per os LD
50＞10000mg/kg, " tealeaves chemistry " 11 (1) .P67 in 1991 have reported the ichthyotoxin activity of Tea Saponin, point out the Tea Saponin of two kinds of models, can be used as the medicament of removing fish in the pond in preceding clear pond of prawn culturing or the breed, but effect are general.The physical and chemical parameter and the multiple use thereof of the unexposed used Tea Saponin of above-mentioned prior art.
The invention provides the Tea Saponin of a kind of efficient removing cultured prawn pond Mesichthyes.This Tea Saponin also is applicable to the larval growth of killing housefly and suppressing housefly.
Sasangua-seed-saponin of the present invention (OTS) is to adopt Chinese patent application number 97100513.3 disclosed methods to extract.Contain sugared body and theasapogenin in this Sasangua-seed-saponin.Main after tested physical and chemical parameter is as follows: belong to the Tea Saponin compounds through the infrared spectrum analysis.Through the uv atlas analysis, at 210nm and 260nm place two absworption peaks are arranged, represent that Sasangua-seed-saponin of the present invention is the Tea Saponin compounds.Prove that through the DSC thermal analysis test Sasangua-seed-saponin of the present invention is 5 kinds of saponin mixtures, the fusing point of each component is respectively 172,190,204,218,228 ℃ in this mixture.Through the high pressure liquid chromatography test shows, Sasangua-seed-saponin of the present invention is the mixture with 5 different components.The shared content of each component is as follows:
Honeybee appearance time (branch) content (%)
1 1.355 0.2125
2 1.775 9.7871
3 2.608 15.1886
4 4.868 31.7857
5 6.363 43.0261
Above-mentioned data show that Sasangua-seed-saponin of the present invention is the mixture of five kinds of Tea Saponins.
Sasangua-seed-saponin of the present invention, through Chinese Medical Sciences University's test, its animal acute toxicity result is LD
501995mg/kg, Lc
50Be 20g/cm
3, Sasangua-seed-saponin of the present invention is a kind of as the desirable medicament of safety of catching and killing the fish of prawn in clear pond before the prawn culturing or the removing breed pond.And the biological feed clam worm of prawn is not had influence.Sasangua-seed-saponin of the present invention has tangible poisoning and the inhibition effect of growing to common house-fly grub.
Embodiment 1,
Sasangua-seed-saponin of the present invention (OTS) is to the toxicity test of line thin white silk used in ancient China goby, line thin white silk used in ancient China goby (Tridentigertrigonocephalus Gill) catches in breed pond, Chinese Academy of Sciences Jiaozhou Bay Ecosystem experiment station, body length is 3.0～7.5cm, 25.9～26.4 ℃ of test seawater water temperatures, salinity 31.91, test is at the 1m of experiment station
3Carry out every group 20 tail, totally 5 groups in the water body cement pit.Inflation continues to observe 48h.The results are shown in table 1.
Table 1 OTS to toxicity OTS concentration (ppm) 0.1 0.2 0.3 0.4 0.5 death toll 0 10 19 20 20 death times (h) 21 13 96 embodiment 2 of line thin white silk used in ancient China goby,
OTS is to the toxicity test of pure fine stern goby, and pure fine stern goby catches sea before Qingdao City's Huang Island, and body is long to be 11.2-20.9cm, and test seawater water temperature is 24.4-26.2 ℃, and salinity is 31.83.Test site, container are the same, every group 10 tail, and totally 5 groups, inflation continues to observe 48h.The results are shown in table 2.
Table 2 OTS to toxicity OTS concentration (ppm) 0.1 0.2 0.3 0.4 0.5 death toll 07 10 10 10 death times (h) 33 20 12 9 embodiment 3 of pure fine stern goby,
Perch comes from mariculture proving ground, the peaceful angle of fishery engineering research institute of Chinese aquatic science institute, the long 8.4-15.2cm of body, and test seawater water temperature is 22.5-22.8 ℃, salinity is 32.56.Test is carried out in the pond of ocean institute of Chinese Academy of Sciences Shui nationality building 40L water body, and totally 5 groups, 1-3 organizes 5 tails, and 4-5 organizes 6 tails, and inflation continues to observe 48h.The results are shown in table 3.
Table 3 OTS to toxicity group 1234 5OTS concentration (ppm), 0.1 0.2 0.3 0.4 0.5 death toll 11466 death times (h) 29 13 66 4.5 embodiment 4 of perch,
OTS tests young shrimp and comes from prawn seed-rearing field, building, back, west, Liu Ting town, Laoshan county, Qingdao City the toxicity test of prawn, the average 2.3cm of biology length, and 23-24 ℃ of test seawater water temperature, salinity is 32.64.Test is carried out in the 8L water body glass jar of laboratory, Chinese Academy of Sciences ocean institute Shui nationality building, and totally 6 groups, every group 10 tail, inflation was observed 48 hours continuously, the results are shown in table 4.
Table 4 OTS to toxicity group 12345 6OTS concentration (ppm), 30 40 50 60 70 100 death toll 025348 death times (h) 40 40 40 40 48 embodiment 5 of prawn,
OTS is to the toxicity test of clam worm, and test clam worm source is the same, the long 20-41cm of body, 14.2 ℃ of seawater water temperatures of test, salinity 32.43.Test is carried out in the glass jar of laboratory, ocean institute Shui nationality building, and the cylinder bottom shop is husky, is divided into 6 groups, and 8 every group, do not inflate continuous observation 48h, the results are shown in table 5.
Table 5 OTS to toxicity OTS concentration (ppm) 2 2.5 3 3.5 4 4.5 death toll 000000 embodiment 6 of clam worm,
OTS of the present invention is mixed with 0.5% the aqueous solution, soaks the wheat bran of cultivating housefly with this, the housefly incubation rate sees Table 6.Embodiment 7 (Comparative Examples),
With the wheat bran of water logging bubble cultivation housefly, the fly incubation rate sees Table 6.
Table 6 OTS is incubated piece weight amount incubation rate after four days to the embodiment that influences of housefly incubation rate
(mg) (number) (larva number) (%) embodiment 6 17 228 00 (0.5%OTS) embodiment 7 17 228 228 100 (water) embodiment 8,
OTS of the present invention is mixed with 0.5% the aqueous solution, soaks the wheat bran of cultivating housefly with this toxic effect of housefly first-instar young is seen Table 7.Embodiment 9 (Comparative Examples),
The wheat bran of cultivating housefly with water logging bubble sees Table 7 to the toxic effect of housefly first-instar young.
Table 7 OTS is to the toxic effect of housefly first-instar young
Total borer population lethality (%)
(head) 2 days 4 days embodiment 8 (0.5%OTS) 400 100 100 embodiment 9 (water) 200 44.5 45.5 embodiment 10,
OTS of the present invention is mixed with 0.5% the aqueous solution, soaks the wheat bran of cultivating housefly with this toxic effect of housefly second instar larvae is seen Table 8.Embodiment 11,
The wheat bran of cultivating housefly with water logging bubble sees Table 8 to the toxic effect of housefly second instar larvae.
Table 8 OTS is to the toxic effect of housefly second instar larvae
Total borer population 2 days 4 days 6 days
(head) lethality larva weight/lethality larva weight/lethality
(%) (mg) (%) (mg) (%) embodiment 10 120 66.7 4.37 95.8 4.00 100 embodiment 11 60 8.3 14.6 13.3 23.5 15
Claims (8)
1, a kind of Sasangua-seed-saponin contains sugared body and theasapogenin, it is characterized in that described Sasangua-seed-saponin is the Tea Saponin mixture of 5 kinds of components, and the fusing point of each component is respectively 172,190,204,218,228 ℃ in the described mixture.
2, the purposes of a kind of Sasangua-seed-saponin according to claim 1 is characterized in that the medicament as clear pond before the prawn culturing.
3, the purposes of a kind of Sasangua-seed-saponin according to claim 1 is characterized in that culturing the fish of catching and killing prawn in the pond as removing.
4, the purposes of a kind of Sasangua-seed-saponin according to claim 3 is characterized in that as removing line thin white silk used in ancient China goby in the shrimp culture pond.
5, the purposes of a kind of Sasangua-seed-saponin according to claim 3 is characterized in that as removing pure fine stern goby in the shrimp culture pond.
6, the purposes of a kind of Sasangua-seed-saponin according to claim 3 is characterized in that as removing perch in the shrimp culture pond.
7, the purposes of a kind of Sasangua-seed-saponin according to claim 1 is characterized in that the medicament as the poisoning housefly.
8, the purposes of a kind of Sasangua-seed-saponin according to claim 1 is characterized in that as suppressing the medicament that common house-fly grub grows.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100066A CN1191069A (en) | 1997-02-21 | 1997-02-21 | Sasangua-seed-saponin and its use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100066A CN1191069A (en) | 1997-02-21 | 1997-02-21 | Sasangua-seed-saponin and its use |
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| CN1191069A true CN1191069A (en) | 1998-08-26 |
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| CN97100066A Pending CN1191069A (en) | 1997-02-21 | 1997-02-21 | Sasangua-seed-saponin and its use |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100431424C (en) * | 2006-12-06 | 2008-11-12 | 福州大学 | Preparation method and use of a fish toxic made of tea saponin |
| CN101904335A (en) * | 2009-06-08 | 2010-12-08 | 上海市园林科学研究所 | Preventive treatment medicine of lawn earthworms as well as preparation method and use method thereof |
| CN102396457A (en) * | 2011-12-10 | 2012-04-04 | 福建农林大学 | Pesticide composition containing tea saponin and oligosaccharins |
-
1997
- 1997-02-21 CN CN97100066A patent/CN1191069A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100431424C (en) * | 2006-12-06 | 2008-11-12 | 福州大学 | Preparation method and use of a fish toxic made of tea saponin |
| CN101904335A (en) * | 2009-06-08 | 2010-12-08 | 上海市园林科学研究所 | Preventive treatment medicine of lawn earthworms as well as preparation method and use method thereof |
| CN102396457A (en) * | 2011-12-10 | 2012-04-04 | 福建农林大学 | Pesticide composition containing tea saponin and oligosaccharins |
| CN102396457B (en) * | 2011-12-10 | 2013-10-23 | 福建农林大学 | Pesticide composition containing tea saponin and oligosaccharins |
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