CN102396457A - Pesticide composition containing tea saponin and oligosaccharins - Google Patents
Pesticide composition containing tea saponin and oligosaccharins Download PDFInfo
- Publication number
- CN102396457A CN102396457A CN2011104130602A CN201110413060A CN102396457A CN 102396457 A CN102396457 A CN 102396457A CN 2011104130602 A CN2011104130602 A CN 2011104130602A CN 201110413060 A CN201110413060 A CN 201110413060A CN 102396457 A CN102396457 A CN 102396457A
- Authority
- CN
- China
- Prior art keywords
- tea saponin
- amino
- oligosaccharide
- disease
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a biogenic pesticide composition with obvious synergistic effect. The active ingredient of the pesticide composition consists of saponin and oligosaccharins. The pesticide composition can be prepared into a water solution, soluble powder, soluble granules, soluble tablets and the like and can be used for preventing and controlling diseases and postharvest diseases in fruit trees, vegetables, tobacco and the like. Actual use results prove that the water solution prepared by the pesticide composition has the characteristics of very obvious synergistic effect, safety for plants and the like.
Description
Technical field
The present invention relates to a kind of composition pesticide, is to be active ingredient with Tea Saponin and amino-oligosacchride, is used for controlling plant diseases.
Background technology
Tea Saponin (Teasaponin) is claimed tea saponin or tea saponin again, by Camellia (
Camellia) plant, as oil tea (
Camellia oleifera), sharp leaf camellia (
C.cuspidatd), the tea plum (
C.sasanqua), tea (
C.sinensis)Deng blade, the glycoside compounds of a kind of pentacyclic triterpene that the tea seed extracts, can be used for preventing and treating the plant disease that causes by multiple fungus.It is to rat acute per os toxicity LD
50Be 4487mg/kg, acute dermal toxicity LD
50>10000mg/kg belongs to low toxicity level.
Tea Saponin has action of contace poison and stomach poison function to damage by disease and insect, but Tea Saponin quick-acting is poor, application dosage is big, and result of use is bad separately.
Amino-oligosaccharide (oligosaccharins); Chemical name is oligomeric-D-glucosamine; Be that a kind of degree of polymerization that next polysaccharide biological species natural products produces through enzymolysis by extracting in the marine organisms chitin is 2 ~ 15 oligomer; Extremely low to pathogenic fungus toxicity, but have the disease-resistant function of inducing plant, can be used as fungus, bacterium and virus disease that the disease resistance of plant derivant is used to prevent and treat various plants such as grain, vegetables, melon, fruit tree, traditional Chinese medicine; The gray mold that the eqpidemic disease that the viroses of plant that wherein viruses such as TMV, CMV caused, oomycetes cause, white eqpidemic disease and botrytis cause etc. has the better prevention effect.To rat acute per os toxicity LD
50Greater than 16000mg/kg, belong to little poison level.
Not relevant is the composition of active ingredient with Tea Saponin and amino-oligosacchride at present, is used for the bibliographical information of controlling plant diseases.
Summary of the invention
The object of the present invention is to provide a kind of safety, environmental protection, effectively contain the biogenic synergetic pesticide composition of Tea Saponin and amino-oligosaccharide.
Technical scheme of the present invention is described below:
A kind of composition pesticide of the present invention, its active ingredient is made up of Tea Saponin and amino-oligosaccharide.
Tea Saponin and amino-oligosaccharide are by mass percentage in the active ingredient: Tea Saponin 95% ~ 80%, amino-oligosaccharide 5% ~ 20%.
The quality percentage composition of each component of said composition pesticide is: active ingredient 5 ~ 80%, surplus are auxiliary agent.
During said composition pesticide practical application each component is hybridly prepared into different dosage forms, comprises aqua, soluble powder, soluble granula, soluble tablet.
In above-mentioned formulation processing; Except that active ingredient; Usually all contain auxiliary agent; And also can choose wantonly according to different dosage form and to contain cosolvent, carrier (or filler) and water etc., also can add other auxiliary agents such as antifreezing agent, thickener, stabilizing agent, preservative, adhesive, antifoaming agent, wetting agent or bleeding agent in case of necessity.Above-mentioned auxiliary agent all is materials well known in the art, can obtain through various commercial channel.Above-mentioned additive dosage can screen definite according to the formulation specification requirement according to the routine techniques means by those skilled in the art.
The present invention has the active composition of controlling plant diseases and can be used for preventing and treating the disease on the important crops such as fruit tree, solanaceous vegetable, tobacco and adopt the back fruit disease, like lichee frost eqpidemic disease, lichee sour rot, capsicum epidemic disease, tomato late blight, guava anthracnose, Mango fruit anthracnose, gray mold, tobacco virus, melon virus disease etc.
Though the present invention have the active composition of controlling plant diseases amount of application need a situation arises, the kind of emergence period, control crop or cropping pattern, weather condition, method of application etc. are adjusted according to disease; But generally speaking; When using composition (in active ingredient, down together) is converted water and be diluted to 100 mg/kg ~ 140 mg/kg.In practice, when the present composition is aqua, solvable pulvis, solvable granula, soluble tablet etc., imposes on the crop after can the consumption dilution of this composition active constituent content or soak fruit and handle by correspondence according to preparation.
The invention has the advantages that utilizing two kinds of active components to be mixed produces preventing and treating the composite synergistic effect of target plant disease, improved control efficiency, reduced the dispenser cost, thereby effectively reduced the loss that brings because of plant disease.
Embodiment:
Biological Examples 1: suppress the synergistic effect of lichee frost eqpidemic disease
Using 50 ℃ hot water dissolving to become the quality percentage composition in the Tea Saponin powder is that to use 40 ℃ hot water dissolving to become the quality percentage composition be 5% mother liquor to the former powder of 20% mother liquor, amino-oligosaccharide; In amino-oligosaccharide: Tea Saponin=1: 4 (5% amino-oligosaccharide mother liquor, 250 mL, 20% Tea Saponin mother liquor, 250 mL), amino-oligosaccharide: Tea Saponin=1: 7 (5% amino-oligosaccharide mother liquor, 200 mL, 20% Tea Saponin mother liquor, 350 mL), amino-oligosaccharide: Tea Saponin=1: 10 (5% amino-oligosaccharide mother liquor, 150 mL, 20% Tea Saponin mother liquor, 375 mL), amino-oligosaccharide: Tea Saponin=1: 13 (5% amino-oligosaccharide mother liquor, 100 mL, 20% Tea Saponin mother liquor, 325 mL), amino-oligosaccharide: Tea Saponin=1: 16 (5% amino-oligosaccharide mother liquor, 100 mL, 20% Tea Saponin mother liquor, 400 mL), amino-oligosaccharide: the ratio mixing of Tea Saponin=1: 19 (5% amino-oligosaccharide mother liquor, 100 mL, 20% Tea Saponin mother liquor, 475 mL) becomes the admixing medical solutions of 6 different proportionings.On the basis of preliminary experiment, obtain inhibition effect the concentration 20% ~ 90% between of each proportioning admixing medical solutions to litchi fruits frost eqpidemic disease.The admixing medical solutions of 6 different proportionings; According to the preliminary experiment result; To be that about 20% concentration is decided to be least concentration, will be decided to be maximum concentration to the concentration of inhibition effect about 90% of litchi fruits frost eqpidemic disease to the inhibition effect of litchi fruits frost eqpidemic disease; From the maximum concentration to the least concentration, by 1/3 ~ 2/3 concentration diminishing method, the admixing medical solutions of 6 different proportionings is mixed with 5 series concentration test solutions respectively with distilled water.Separation from litchi fruits and through the peronophythora litchi of pathogenic mensuration (
Peronophthora litchii) bacterial classification, cultivate the generation sporangium with plating medium; Adopt ripe anosis worm litchi fruits,, be divided into 28 parts after drying through rinsed with sterile water; 50 every part, drop into 5 series concentration test solutions, sterile water (2 parts) respectively, soak 3 min; After taking-up is dried; Place the basin of preserving moisture to move into the biochemical incubator of (22 ± 1) ℃, behind 48 h, contain sporangium 2.5 * 10 with every milliliter
4Individual peronophythora litchi sporangia suspension implements to connect bacterium to the litchi fruits of 5 series concentration test solutions and 1 part of sterile water processing; 4 repetitions are set; Connect the biochemical incubator that places the basin of preserving moisture to move into (25 ± 1) again behind the bacterium and cultivate, handle back 4 d inspection fruit disease index.Sick fruit grade scale is 0 grade: anosis; 1 grade: scab accounts for the fruit area below 10%; 3 grades: scab accounts for fruit area 11%～25%; 5 grades: scab accounts for fruit area 26%～50%; 7 grades: scab accounts for the fruit area more than 50%.Calculate each disease index of handling of each medicament:
With medicament the inhibiting rate of disease is represented to suppress effect, calculates the inhibiting rate of medicament disease according to the disease index of each processing:
Convert the dosage of medicament to logarithm value, inhibiting rate converts the probability value to, with DPS computed in software EC
50And virulence regression equation.Obtain the co-toxicity coefficient (CTC) of each mixed ratio with reference to the method for NY/T1154.7-2006.The result is as shown in table 1:
Table 1: amino-oligosaccharide and Tea Saponin are mixed litchi fruits frost eqpidemic disease inhibitory action are measured the result
Annotate: supply O-amino-oligosaccharide in the reagent agent, T-Tea Saponin, O: the ratio of quality and the number of copies of T-amino-oligosaccharide and Tea Saponin.
Biological Examples 2: suppress the synergistic effect of guava anthracnose
On the basis of preliminary experiment (to the inhibition effect of disease between 20% ~ 90%); With the female medicine of the method for biological Examples 1 dissolving Tea Saponin and amino-oligosaccharide mother medicine, be mixed into the admixing medical solutions of 6 different proportionings, and the admixing medical solutions of 6 different proportionings is mixed with 5 series concentration test solutions respectively with distilled water; Separation from guava fruit and through the colletotrichum gloeosporioides Penz of pathogenic mensuration (
Colletotrichum gloeosporioides) bacterial classification, cultivate the generation spore with plating medium.Adopt ripe guava fruit, through rinsed with sterile water, be divided into 28 parts after drying, 30 every part; Drop into 5 series concentration test solutions, sterile water (2 parts) respectively, soak 3 min, after taking-up is dried; Place the plastic box of adding a cover, behind the 48h, contain conidium 4.0 * 10 with every milliliter with fiber board with holes
6The guava fruit that individual guava anthrax-bacilus spore suspension is handled 5 series concentration test solutions and 1 part of sterile water is implemented to connect bacterium, connects bacterium and is placed in the biochemical incubator that moves into (25 ± 1) ℃ in the basin of preserving moisture and cultivates, and handles back 7d inspection fruit disease index.Sick fruit grade scale is 0 grade: anosis; 1 grade: scab accounts for the fruit area below 5%; 3 grades: scab accounts for fruit area 6%～15%; 5 grades: scab accounts for fruit area 16%～25%; 7 grades: scab accounts for fruit area 26%～25%; 9 grades: scab accounts for the fruit area more than 50%.Calculate each disease index of handling of each medicament:
With medicament the inhibiting rate of disease is represented to suppress effect, calculates the inhibiting rate of medicament disease according to the disease index of each processing:
Calculate the inhibiting rate of medicament to disease, convert the dosage of medicament to logarithm value, inhibiting rate converts the probability value to, with DPS computed in software EC
50And virulence regression equation.Obtain the co-toxicity coefficient (CTC) of each mixed ratio with reference to the method for NY/T1154.7-2006.The result is as shown in table 2:
Table 2: amino-oligosaccharide and Tea Saponin are mixed guava fruit anthracnose inhibitory action are measured the result
Annotate: supply O-amino-oligosaccharide in the reagent agent, T-Tea Saponin, O: the ratio of quality and the number of copies of T-amino-oligosaccharide and Tea Saponin.
1-2 can find out by table; Can significantly improve control efficiency after Tea Saponin and amino-oligosaccharide are composite to lichee frost eqpidemic disease, guava anthracnose; At the mass ratio of amino-oligosaccharide and Tea Saponin in 1: 4 to 1: 19 scope; The control efficiency of lichee frost eqpidemic disease is increased by 1.52 ~ 2.98 times, the control efficiency of guava anthracnose is increased by 1.48 ~ 2.85 times, and wherein with amino-oligosaccharide: the synergistic effect of Tea Saponin=1: 16 is the most remarkable.
Among the embodiment below, composition pesticide of the present invention is further explained, but it never constitutes restriction to scope of the present invention.In addition, in following examples used part all or ratio be mass fraction or mass ratio.
FORMULATION EXAMPLE 1
8 parts of Tea Saponins (conversion is 100% for the active component mass fraction)
0.5 part of amino-oligosaccharide (conversion is 100% for the active component mass fraction)
Auxiliary agent (propane diols, sorbic acid) 4+3=7 part
Water complements to 100 parts
After above-mentioned each composition dissolved respectively, mixing was processed 8.5% amino-oligosaccharide Tea Saponin aqua.This preparation can be prevented and treated plant diseases such as lichee frost eqpidemic disease, guava anthracnose effectively.
FORMULATION EXAMPLE 2
56 parts of Tea Saponins (conversion is 100% for the active component mass fraction)
3.5 parts of amino-oligosaccharides (conversion is 100% for the active component mass fraction)
1.5 parts of sodium lignin sulfonates
1.5 parts in alkylnaphthalene sulfonate formaldehyde
1.0 parts in sodium sulphate
White carbon+precipitated calcium carbonate (1: 1) complements to 100 parts
Tea Saponin, amino-oligosaccharide are crushed to 98% are crushed to 98% respectively through behind the 45 μ m standard screens,, process 59.5% amino-oligosaccharide Tea Saponin soluble powder by above-mentioned mixing respectively through 75 μ m standard screens, above-mentioned auxiliary agent.This preparation can be prevented and treated plant diseases such as lichee frost eqpidemic disease, guava anthracnose effectively.
Be used to prevent and treat the embodiment of litchi fruits frost eqpidemic disease
In the litchi fruits preceding 8d that gathers, the spraying control litchi fruits frost eqpidemic disease in the orchard.Get 8.5% amino-oligosaccharide Tea Saponin aqua, 40 mL that FORMULATION EXAMPLE 1 processes, impouring is equipped with in the Plastic Drum of 15L clear water, with waddy rotation stir about 3min, adds clear water 10 L again, with waddy rotation stir about 2min, becomes soup.Be contrast with 1.35% amino-oligosaccharide aqua (theoretical toxicity index is equivalent to 8.5% amino-oligosaccharide Tea Saponin), 40 mL, 12.7% Tea Saponin aqua (theoretical toxicity index is equivalent to 8.5% amino-oligosaccharide Tea Saponin), 40 mL, clear water respectively, the method for preparating liquid is with above-mentioned.Every processing sprays 2 strains, and 4 repetitions are set, and adopts the stretcher type power driven sprayer be furnished with 0.8mm aperture shower nozzle, aims at the litchi fruit ear, soup is evenly sprayed application on the fruit of litchi, is covered with droplet and only has and drip to ground degree of being under a small amount of soup with fruit.Each disease index of handling of 7d investigation before the dispenser, after the dispenser is pressed the method for pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating peronophythora litchi (GB/T 17980.100-2004) and is calculated control efficiency.The control efficiency that 8.5% amino-oligosaccharide Tea Saponin aqua reaches 84.5%, 1.35% amino-oligosaccharide aqua to the control efficiency of litchi fruits frost eqpidemic disease is that the control efficiency of 65.2%, 12.7% Tea Saponin aqua is 59.3%.This presentation of results, 8.5% amino-oligosaccharide Tea Saponin aqua control peronophythora litchi is than using Tea Saponin and amino-oligosaccharide can improve 19.3%, 25.2% respectively separately.After dispenser,, do not have the amino-oligosaccharide of discovery Tea Saponin lichee blade, fruit are produced harmful effect the fixed point observation of litchi.
Be used to prevent and treat the embodiment of guava fruit anthracnose
After guava fruit is gathered, the 8.5% amino-oligosaccharide Tea Saponin aqua 30mL that uses FORMULATION EXAMPLE 1 to process, impouring is equipped with in the Plastic Drum of 15L clear water; With waddy rotation stir about 3min; Add clear water 10 L again,, become soup with waddy rotation stir about 2min.Be contrast with 1.6% amino-oligosaccharide aqua (theoretical toxicity index is equivalent to 8.5% amino-oligosaccharide Tea Saponin), 30 mL, 11.6% Tea Saponin aqua (theoretical toxicity index is equivalent to 8.5% amino-oligosaccharide Tea Saponin), 30 mL, clear water respectively, the method for preparating liquid is with above-mentioned.Worm, disease, the wound of guava fruit are really detected, the strong fruit of 30 of every processing, the mesh bag about 3 cm of aperture of packing into places plastic box, and every processing is provided with 4 repetitions.The treatment fluid for preparing injected the plastic box of guava is housed, make the soup face be higher than about 10 cm of guava fruit layer, soak 5 min after, mesh bag is proposed, make to drop down under the fruit face liquid medicine to no obvious liquid medicine and drip.After the processing, Fructus psidii guajavae immaturus in the mesh bag is taken out individual layer spread out on the rack, dry the back vanning.10 d " Invest, Then Investigate "s calculate control efficiency to the control efficiency of Fructus psidii guajavae immaturus by the method for pesticide field efficacy medicine test criterion (two) bactericidal agent for preventing and treating mango anthracnose (adopting the rear section) (GB/T 17980.99-2004).The control efficiency that 8.5% amino-oligosaccharide Tea Saponin aqua reaches 90.4%, 1.6% amino-oligosaccharide aqua to the control efficiency of Fructus psidii guajavae immaturus anthracnose is that the control efficiency of 71.6%, 11.6% Tea Saponin aqua is 63.7%.This presentation of results, 8.5% amino-oligosaccharide Tea Saponin aqua control guava anthracnose is than using Tea Saponin and amino-oligosaccharide can improve 19.3%, 25.2% respectively separately.Regularly observe after treatment, the practical amino-oligosaccharide Tea Saponin of Fructus psidii guajavae immaturus is handled back 15d, does not still have the fruit of discovery medicine spot or unusual variable color are arranged.
Claims (4)
1. composition pesticide, its active ingredient is made up of Tea Saponin and amino-oligosaccharide.
2. composition pesticide according to claim 1 is characterized in that: Tea Saponin and amino-oligosaccharide are by mass percentage in the active ingredient: Tea Saponin 95%-80%, amino-oligosaccharide 5%-20%.
3. composition pesticide according to claim 1 is characterized in that: the quality percentage composition of each component of said composition pesticide is: active ingredient 5-80%, surplus is an auxiliary agent.
4. composition pesticide according to claim 3 is characterized in that: during said composition pesticide practical application each component is hybridly prepared into different dosage forms, comprises aqua, soluble powder, soluble granula, soluble tablet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110413060 CN102396457B (en) | 2011-12-10 | 2011-12-10 | Pesticide composition containing tea saponin and oligosaccharins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110413060 CN102396457B (en) | 2011-12-10 | 2011-12-10 | Pesticide composition containing tea saponin and oligosaccharins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102396457A true CN102396457A (en) | 2012-04-04 |
| CN102396457B CN102396457B (en) | 2013-10-23 |
Family
ID=45879388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110413060 Expired - Fee Related CN102396457B (en) | 2011-12-10 | 2011-12-10 | Pesticide composition containing tea saponin and oligosaccharins |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102396457B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659472A (en) * | 2012-04-26 | 2012-09-12 | 江南大学 | Enhanced chitosan oligosaccharide plant growth modifier formula containing plant saponin |
| CN104686518A (en) * | 2015-02-06 | 2015-06-10 | 中国农业科学院植物保护研究所 | Biological compound preparation containing m-methoxybenzoic acid and amino-oligosaccharin and application of biological compound preparation |
| CN106259346A (en) * | 2016-08-20 | 2017-01-04 | 李祥英 | A kind of containing fluopyram and the nematicidal composition of tea saponin |
| CN107711844A (en) * | 2017-09-30 | 2018-02-23 | 京博农化科技股份有限公司 | A kind of composition containing amino-oligosaccharide and plant growth regulator |
| CN109497095A (en) * | 2019-01-03 | 2019-03-22 | 临沂大学 | A kind of biological pesticide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191069A (en) * | 1997-02-21 | 1998-08-26 | 中国科学院化学研究所 | Sasangua-seed-saponin and its use |
| CN1327744A (en) * | 2001-06-15 | 2001-12-26 | 青岛海普生物技术有限公司 | Theasaponin-chitosan compound and its preparing process and application in insecticide |
| JP2004203771A (en) * | 2002-12-25 | 2004-07-22 | Nihon Medi Physics Co Ltd | Method for separating metal complex of aminooligosaccharide derivative from metal complex of bifunctional ligand |
| CN101124916A (en) * | 2007-08-06 | 2008-02-20 | 大连奥德植保药业有限公司 | Biological pesticide mixed with plant insecticide sterilization component and amino-oligosaccharin and preparation method thereof |
-
2011
- 2011-12-10 CN CN 201110413060 patent/CN102396457B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191069A (en) * | 1997-02-21 | 1998-08-26 | 中国科学院化学研究所 | Sasangua-seed-saponin and its use |
| CN1327744A (en) * | 2001-06-15 | 2001-12-26 | 青岛海普生物技术有限公司 | Theasaponin-chitosan compound and its preparing process and application in insecticide |
| JP2004203771A (en) * | 2002-12-25 | 2004-07-22 | Nihon Medi Physics Co Ltd | Method for separating metal complex of aminooligosaccharide derivative from metal complex of bifunctional ligand |
| CN101124916A (en) * | 2007-08-06 | 2008-02-20 | 大连奥德植保药业有限公司 | Biological pesticide mixed with plant insecticide sterilization component and amino-oligosaccharin and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 《农药学学报》 20060330 刘幸海 等 具有农业生物活性壳寡糖的研究进展 第1-6页 1-4 第8卷, 第1期 * |
| 刘幸海 等: "具有农业生物活性壳寡糖的研究进展", 《农药学学报》, vol. 8, no. 1, 30 March 2006 (2006-03-30), pages 1 - 6 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659472A (en) * | 2012-04-26 | 2012-09-12 | 江南大学 | Enhanced chitosan oligosaccharide plant growth modifier formula containing plant saponin |
| CN104686518A (en) * | 2015-02-06 | 2015-06-10 | 中国农业科学院植物保护研究所 | Biological compound preparation containing m-methoxybenzoic acid and amino-oligosaccharin and application of biological compound preparation |
| CN106259346A (en) * | 2016-08-20 | 2017-01-04 | 李祥英 | A kind of containing fluopyram and the nematicidal composition of tea saponin |
| CN107711844A (en) * | 2017-09-30 | 2018-02-23 | 京博农化科技股份有限公司 | A kind of composition containing amino-oligosaccharide and plant growth regulator |
| CN109497095A (en) * | 2019-01-03 | 2019-03-22 | 临沂大学 | A kind of biological pesticide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102396457B (en) | 2013-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103518750B (en) | High-efficiency compound biological bactericide and application thereof | |
| CN102396457B (en) | Pesticide composition containing tea saponin and oligosaccharins | |
| CN102894002B (en) | Compound bactericide containing berberine and polyoxins and application thereof | |
| CN104322558A (en) | Sterilization composition and applications thereof | |
| CN105660659A (en) | Bactericide composition | |
| CN106508970A (en) | Compound sterilizing composition and application thereof | |
| CN103766353A (en) | Sterilization composition containing fluopyram | |
| CN103348982B (en) | A kind of Fungicidal mixture | |
| CN104817406A (en) | Plant protection composition containing chitosan oligosaccharide and dinotefuran | |
| CN105309512A (en) | Compound biopesticide for controlling powdery mildew of hazelnuts and preparing method thereof | |
| CN104186513A (en) | Herbicidal composition and preparation thereof | |
| CN109769876A (en) | A kind of complex biological pesticide and preparation method thereof for preventing and treating apple tree canker | |
| CN103493832B (en) | Efficient complex biological fungicide and application thereof | |
| CN106665623A (en) | Sterilization composition containing picarbutrazox and application thereof | |
| CN102150675B (en) | Bactericidal composition containing captan and thiocatbamate compound | |
| CN105010368B (en) | Composition pesticide and its application | |
| CN101647439B (en) | Mixed sterilization combination containing boscalid | |
| CN104365611B (en) | A kind of bactericidal composition and application containing amisulbrom and mandipropamid | |
| CN1994083B (en) | A compound bactericidal agent | |
| CN105432639B (en) | A kind of bactericidal composition for preventing and treating vegetable disease and application | |
| CN103478129A (en) | Agricultural compound bactericide and application thereof | |
| CN103478153A (en) | Sterile composition containing boscalid and benthiavalicarb isopropyl | |
| CN106359455A (en) | Paecilomyces lilacinus wettable powder and preparation method thereof | |
| CN101822256A (en) | Sterilization compound containing ZJ0712 | |
| CN101743979B (en) | Bactericidal composite with active ester |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131023 Termination date: 20161210 |