CN101647439B - Mixed sterilization combination containing boscalid - Google Patents
Mixed sterilization combination containing boscalid Download PDFInfo
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- CN101647439B CN101647439B CN2009101614902A CN200910161490A CN101647439B CN 101647439 B CN101647439 B CN 101647439B CN 2009101614902 A CN2009101614902 A CN 2009101614902A CN 200910161490 A CN200910161490 A CN 200910161490A CN 101647439 B CN101647439 B CN 101647439B
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- boscalid
- procymidone
- dimethachlon
- days
- sclex
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- 239000005740 Boscalid Substances 0.000 title claims abstract description 113
- WYEMLYFITZORAB-UHFFFAOYSA-N boscalid Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC=C1NC(=O)C1=CC=CN=C1Cl WYEMLYFITZORAB-UHFFFAOYSA-N 0.000 title claims abstract description 113
- 229940118790 boscalid Drugs 0.000 title claims abstract description 113
- 230000001954 sterilising effect Effects 0.000 title abstract description 6
- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 5
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims description 38
- 230000000844 anti-bacterial effect Effects 0.000 claims description 20
- 239000000375 suspending agent Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 15
- 238000009736 wetting Methods 0.000 claims description 15
- 239000004562 water dispersible granule Substances 0.000 claims description 13
- 239000004480 active ingredient Substances 0.000 claims description 10
- 238000009472 formulation Methods 0.000 claims description 3
- 241000607479 Yersinia pestis Species 0.000 claims 1
- JDZSMXLTQNHBRF-UHFFFAOYSA-N Dichlozoline Chemical compound O=C1C(C)(C)OC(=O)N1C1=CC(Cl)=CC(Cl)=C1 JDZSMXLTQNHBRF-UHFFFAOYSA-N 0.000 abstract description 27
- CFZLNRGUBAVQNO-UHFFFAOYSA-N N-(3,5-Dichlorophenyl)succinimide Chemical compound ClC1=CC(Cl)=CC(N2C(CCC2=O)=O)=C1 CFZLNRGUBAVQNO-UHFFFAOYSA-N 0.000 abstract description 27
- 201000010099 disease Diseases 0.000 abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 7
- 235000013399 edible fruits Nutrition 0.000 abstract description 6
- 229920000742 Cotton Polymers 0.000 abstract description 2
- 244000299507 Gossypium hirsutum Species 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract description 2
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 2
- 235000021307 Triticum Nutrition 0.000 abstract description 2
- 238000002156 mixing Methods 0.000 abstract description 2
- 235000009566 rice Nutrition 0.000 abstract description 2
- 235000013311 vegetables Nutrition 0.000 abstract description 2
- 239000005759 Diethofencarb Substances 0.000 abstract 1
- 241000209140 Triticum Species 0.000 abstract 1
- LNJNFVJKDJYTEU-UHFFFAOYSA-N diethofencarb Chemical compound CCOC1=CC=C(NC(=O)OC(C)C)C=C1OCC LNJNFVJKDJYTEU-UHFFFAOYSA-N 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 24
- 238000000034 method Methods 0.000 description 19
- 230000002195 synergetic effect Effects 0.000 description 18
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 15
- 239000002131 composite material Substances 0.000 description 15
- 230000003449 preventive effect Effects 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 231100000419 toxicity Toxicity 0.000 description 12
- 230000001988 toxicity Effects 0.000 description 12
- 240000008067 Cucumis sativus Species 0.000 description 11
- 239000003795 chemical substances by application Substances 0.000 description 11
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 10
- 240000002791 Brassica napus Species 0.000 description 7
- 235000006008 Brassica napus var napus Nutrition 0.000 description 7
- 241000221662 Sclerotinia Species 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 241000123650 Botrytis cinerea Species 0.000 description 6
- 230000003902 lesion Effects 0.000 description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 5
- 240000003768 Solanum lycopersicum Species 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 231100000820 toxicity test Toxicity 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 239000000440 bentonite Substances 0.000 description 3
- 229910000278 bentonite Inorganic materials 0.000 description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000009036 growth inhibition Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 235000014347 soups Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000005995 Aluminium silicate Substances 0.000 description 2
- 241000222290 Cladosporium Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 235000016623 Fragaria vesca Nutrition 0.000 description 2
- 240000009088 Fragaria x ananassa Species 0.000 description 2
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 2
- 244000061176 Nicotiana tabacum Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 235000012211 aluminium silicate Nutrition 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000002270 dispersing agent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- XUZHLZDRCCUWEV-UHFFFAOYSA-N formaldehyde;methyl naphthalene-1-sulfonate Chemical compound O=C.C1=CC=C2C(S(=O)(=O)OC)=CC=CC2=C1 XUZHLZDRCCUWEV-UHFFFAOYSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- 229920001285 xanthan gum Polymers 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 229960000892 attapulgite Drugs 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010205 computational analysis Methods 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- SYHPANJAVIEQQL-UHFFFAOYSA-N dicarboxy carbonate Chemical compound OC(=O)OC(=O)OC(O)=O SYHPANJAVIEQQL-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 150000002466 imines Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- AIDQCFHFXWPAFG-UHFFFAOYSA-N n-formylformamide Chemical class O=CNC=O AIDQCFHFXWPAFG-UHFFFAOYSA-N 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 229910052625 palygorskite Inorganic materials 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000009700 powder processing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000011708 vitamin B3 Substances 0.000 description 1
- 235000019160 vitamin B3 Nutrition 0.000 description 1
Abstract
The invention relates to a sterilization combination which contains the effective components of (A) boscalid and (B) which is one of procymidone, diethofencarb, dichlozoline or dimethachlon, wherein the mass proportion of A and B is 30:1 to 1:50. After mixing, A and B have obvious synergism, and are used for preventing and treating diseases of crops such as fruit trees, flowers, cottons, wheats, rices, rapes, vegetables and the like.
Description
Technical field
The present invention relates to a kind of bactericidal composition, especially a kind of combination sterilization composition that contains Boscalid.
Background technology
Gray mold such as gray mold of cucumber and graw mold of tomato etc. are one type of very important diseases on the crops, often cause the heavy losses of crops.Stalk break also is a kind of very important disease, belongs to sclerotium bacteria by Ascomycotina nuclear disk spore and cause that generation is often arranged on rape, cucumber, and at the florescence, environmental condition warm, high humidity is prone to popular, can cause heavy losses.
According to bibliographical information, because single drug and other unscientific application method have caused gray mold, stalk break etc. that common pesticides has been produced resistance, the increase that resistance produces back formulation rate and spraying times causes peasant burden to increase the weight of and increasing environmental pollution again.
Exploitation is efficient, the bactericidal composition of low toxicity, environmental protection can head it off.
Boscalid, English common name boscalid, chemical name: 2-chloro-N-(4 '-chlordiphenyl-2-yl) vitamin PP; It is the novel nicotinamide bactericide of BASF Aktiengesellschaft's exploitation; Fungicidal spectrum is wide, has protection and therapeutic action, can prevent and treat gray mold and stalk break.Boscalid hinders tricarbonic acid cycle, the division of interference cell and growth through suppressing mitochondrial succinate dehydrogenase.Can suppress spore germination, the extension of bacterium pipe, mycelial growth and spore mother cell forms.Bactericidal action is unique, does not have cross resistance with other bactericide.Boscalid is the new and effective medicament of control crop gray mold and stalk break, but use cost is high and the risk of generation resistance arranged separately.
In view of this, the exploitation complex preparation that the contains effective composition Boscalid resistance management that is used for carrying out gray mold, stalk break has suitable urgency and using value.
Summary of the invention
The objective of the invention is to: provide and use on a kind of suitable agricultural, gray mold, stalk break had safety, the efficient bactericidal composite of outstanding control efficiency.
For solving the problems of the technologies described above; The inventor is through a large amount of biologicall test screenings; Unexpected find in (A) Boscalid and (B) procymidone, the mould prestige of second, sclex or the dimethachlon a kind of compositely, gray mold, stalk break are all had significant synergies with certain proportion.
Procymidone, the mould prestige of second, sclex, dimethachlon all are the medicaments commonly used of preventing and treating gray mold and stalk break commonly used at present, and its mode of action is all different with Boscalid.Procymidone and sclex are all diformamide class low toxicity, efficient germicide, and mainly interference cell kernel function, and cell membrane and cell wall are influential, change permeability of the membrane, make cell rupture.The mould prestige of second suppresses the formation of botrytis cinerea gemma spindle.Dimethachlon is an imines class contact bactericide, and certain interior suction osmosis is arranged, and the lasting period is long.
Technical scheme of the present invention is following:
A kind of bactericidal composition; With (A) Boscalid is a kind of active ingredient; With a kind of in (B) procymidone, the mould one-tenth of second, sclex or the dimethachlon be another active ingredient; (A) be 30 with (B) mass ratio: 1-1: 50, ratio is 10 preferably: 1-1: 20, all the other be to allow use and acceptable auxiliary element in the agricultural chemicals.
The present composition can be prepared into known method and be fit to the formulation that agricultural is used, and comprises wetting powder, water dispersible granules, suspending agent, and the total content of active ingredient is 10%-85% in the preparation.
Composition described in the invention can the finished product preparation form provide, and promptly each material mixes in the composition, and the composition of composition also can provide with single agent form, directly in bucket or jar, directly mixes before the use, is diluted to required concentration then.
Bactericidal composition of the present invention is mainly used in disease, the especially gray mold of crops such as fruit tree, flowers, cotton, wheat, paddy rice, rape, vegetables, the control of stalk break.
Compared with prior art, the beneficial effect of the present invention's generation is: (1) is compared with single agent, and this bactericidal composition has tangible synergy to disease, has improved control efficiency; (2) can significantly reduce the field dosage, effectively reduce environmental pollution and residue of pesticide, reduce and produce and use cost; (3) mechanism of action of active ingredient is different in the bactericidal composition, has reduced the single selection pressure to germ, the generation that helps overcoming the disease resistance He delay disease drug resistance.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer, the present invention describes with following specific embodiment, but the present invention is limited to these examples absolutely not.
The combination of different pesticide active ingredient is carried out composite, be solve some problems in the agricultural chemicals list agent application process at present a kind of effectively with mode efficiently.Different pesticide active ingredients shows three kinds of type of action after mixing usually, and promptly summation action, synergistic effect and antagonism act on but which kind of is specially, and be unpredictable, has only through a large amount of tests and just can know.Compound synergic is well filled a prescription, and can improve actual control efficiency, reduces the usage amount of agricultural chemicals, helps the generation of delaying drug resistance, is the important means of scientific prevention and cure.
The present composition with a kind of in (A) Boscalid and (B) procymidone, the mould prestige of second, sclex or the dimethachlon be active ingredient; Combination has obvious role in synergism to gray mold, stalk break between them; And be not only the simple addition of two kinds of medicament effects, specifically explain with following biologicall test instance.
Biologicall test instance 1: the composite toxicity test of the mould prestige of Boscalid and procymidone or second to gray mold of cucumber
Subjects: botrytis cinerea pers (Botrytis cinerea Pers.)
Pot-culture method is adopted in this test.Potted plant cucumber seedling grows to 2 leaf periods, numbers subsequent use.On the basis of preliminary experiment, active according to medicament, 5-7 series mass concentration is set.Soup is evenly sprayed in the blade face to all wetting, be advisable, treat that the soup natural air drying is subsequent use not drip.Every processing 3 basins repeat for 4 times, and establish the processing that only contains solvent and surfactant and do not contain effective composition and make blank.Behind the chemicals treatment 24h, with crop sprayer even spraying inoculation botrytis cinerea pers spore suspension on cucumber leaves.The collocation method of spore suspension is following: test, washes spore with sterile water after being cultured to a large amount of spores of generation on the suitable culture base with pathogen, and double gauze filters, and is modulated into 1 * 10
5Individual spore/mL spore suspension, subsequent use.Move in the humidistat (relative moisture more than 95%, temperature 20-22 ℃) dark condition after the inoculation and cultivate 24h down, under 20-25 ℃, relative moisture 80%-90% condition, cultivated 7 days then.Add up the lesion area on every strain cucumber leaves, whole blades are investigated in every processing, calculate disease and refer to and control efficiency.
The gray mold of cucumber stage division is:
Grade scale
0 grade: blade does not have scab;
1 grade: lesion area accounts for below 5% of whole blade area;
3 grades: lesion area accounts for the 6-15% of whole blade area;
5 grades: lesion area accounts for the 16-25% of whole blade area;
7 grades: lesion area accounts for the 26-50% of whole blade area;
9 grades: lesion area accounts for more than 50% of whole blade area.
The drug effect computational methods:
Control efficiency is converted into probability value (y), and liquor strength (μ g/ml) converts logarithm value (x) to, concentration EC in calculating the virulence equation and suppress with method of least squares
50, according to the toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.
Actual measurement toxicity index (ATI)=(standard medicament EC
50/ confession reagent agent EC
50) * 100
The percentage composition of B in percentage composition+B medicament toxicity index * mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture
Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (the ATI)/theoretical toxicity index (TTI) of mixture] * 100
When CTC≤80, then the composition performance is for antagonism, and when 80＜CTC＜120, then the composition performance is for summation action, and when CTC>=120, then the composition performance is synergistic effect.
Toxicity test result is referring to table 1,2
The serial proportioning of table 1 Boscalid and procymidone is to the Toxicity Determination result of botrytis cinerea pers
| Handle | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Boscalid | 25.86 | 100 | / | / |
| Procymidone | 50.35 | 51.4 | / | / |
| Boscalid 30: procymidone 1 | 19.32 | 133.9 | 98.4 | 136.0 |
| Boscalid 20: procymidone 1 | 17.37 | 148.9 | 97.7 | 152.4 |
| Boscalid 10: procymidone 1 | 13.37 | 193.4 | 95.6 | 202.4 |
| Boscalid 5: procymidone 1 | 11.26 | 229.7 | 91.9 | 249.9 |
| Boscalid 1: procymidone 1 | 12.32 | 209.9 | 75.7 | 277.4 |
| Boscalid 1: procymidone 5 | 14.44 | 179.1 | 59.5 | 301.2 |
| Boscalid 1: procymidone 10 | 19.01 | 136.0 | 55.8 | 243.9 |
| Boscalid 1: procymidone 20 | 23.32 | 110.9 | 53.7 | 206.6 |
| Boscalid 1: procymidone 30 | 33.12 | 78.1 | 52.9 | 147.5 |
| Boscalid 1: procymidone 50 | 37.12 | 69.7 | 52.3 | 133.2 |
Result of the test shows, the composite control gray mold of cucumber of Boscalid and procymidone, and proportioning is 30: 1-1: in the time of between 50; Co-toxicity coefficient all more than 142.9, has synergistic effect, and proportioning is 10: 1-1: in the time of between 20; Co-toxicity coefficient all is higher than 202.4, and synergistic effect is more obvious.
The serial proportioning of table 2 Boscalid and the mould prestige of second is to the Toxicity Determination result of botrytis cinerea pers
| Handle | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Boscalid | 25.86 | 100 | / | / |
| The mould one-tenth of second | 52.35 | 49.4 | / | / |
| Boscalid 30: the mould prestige 1 of second | 18.32 | 141.2 | 98.4 | 143.5 |
| Boscalid 20: the mould prestige 1 of second | 17.33 | 149.2 | 97.6 | 152.9 |
| Boscalid 10: the mould prestige 1 of second | 12.22 | 211.6 | 95.4 | 221.8 |
| Boscalid 5: the mould prestige 1 of second | 11.36 | 227.6 | 91.6 | 248.6 |
| Boscalid 1: the mould prestige 1 of second | 13.09 | 197.6 | 74.7 | 264.5 |
| Boscalid 1: the mould prestige 5 of second | 14.38 | 179.8 | 57.8 | 311.0 |
| Boscalid 1: the mould prestige 10 of second | 19.05 | 135.7 | 54.0 | 251.4 |
| Boscalid 1: the mould one-tenth 20 of second | 24.34 | 106.2 | 51.8 | 205.1 |
| Boscalid 1: the mould prestige 30 of second | 34.17 | 75.7 | 51.0 | 148.3 |
| Boscalid 1: the mould prestige 50 of second | 38.12 | 67.8 | 50.4 | 134.6 |
Result of the test shows, the composite control gray mold of cucumber of the mould prestige of Boscalid and second, and proportioning is 30: 1-1: in the time of between 50; Co-toxicity coefficient has synergistic effect more than 134.6, and proportioning is 10: 1-1: in the time of between 20; Co-toxicity coefficient all is higher than 205.1, and synergistic effect is more obvious.
Biologicall test instance 2: the composite toxicity test of Boscalid and sclex or dimethachlon to sclerotinia rot of colza
Subjects: Sclerotinia sclerotiorum (Sclerotinia sclerotiorum)
Mycelial growth rate method (Plating) is adopted in test.On the basis of preliminary experiment; Handling the sterilising medium that will melt in advance according to test quantitatively places in the aseptic conical flask; From the low concentration to the high concentration, getting the 5ml soup successively joins respectively the hot medium of 45ml (PDA medium is housed; 45-50 ℃) conical flask in, pour diameter 90mm glass culture dish after shaking up rapidly into, each culture dish is poured band medicine medium 10ml into.Level leaves standstill, and processes flat board after the cooling.Five repetitions of each concentration.Blank is made in clear water processing (containing a little solvent and surfactant) not contain effective ingredient.With cultured pathogen, under aseptic condition,, cut the bacterium cake from colony edge with the sterilization card punch of diameter 4mm, with inoculator the bacterium cake is inoculated in the dull and stereotyped central authorities of pastille, will there be one of mycelia to face down and the medium applying, cover the ware lid.Above all operations all carries out sterile working at superclean bench.After the processing flat board is placed in 28 ± 0.5 ℃ the constant temperature aseptic culture case and cultivates, take out behind the 5d.Adopt the right-angled intersection method to measure the colony diameter (is unit with the millimeter) that each is handled respectively, calculate the mean value of colony diameter.Calculate and respectively handle the clean amount of growth of mycelia, mycelial growth inhibition rate.
Clean amount of growth (mm)=measurement colony diameter-4
Mycelial growth inhibition rate (%)=[(the clean amount of growth of the clean amount of growth-processed group of control group)/clean amount of growth of control group] * 100
Mycelial growth inhibition rate is converted into probability value (y), and liquor strength (μ g/ml) converts logarithm value (x) to, concentration EC in calculating the virulence equation and suppress with method of least squares
50, toxicity index, co-toxicity coefficient computational analysis method are the same.
Toxicity test result is referring to table 3,4
The serial proportioning of table 3 Boscalid and sclex is to the Toxicity Determination result of sclerotinia rot of colza
Handle EC
50(μ g/mL) ATI TTI co-toxicity coefficient CTC
Boscalid 0.27 100 //
Sclex 0.53 50.9 //
Boscalid 30: sclex 1 0.20 135.0 98.4 137.2
Boscalid 20: sclex 1 0.19 142.1 97.7 145.5
Boscalid 10: sclex 1 0.15 180.0 95.5 188.4
Boscalid 5: sclex 1 0.13 207.7 91.8 226.2
Boscalid 1: sclex 1 0.12 225.0 75.5 298.1
Boscalid 1: sclex 5 0.17 158.8 59.1 268.6
Boscalid 1: sclex 10 0.21 128.6 55.4 232.1
Boscalid 1: sclex 20 0.27 100.0 53.3 187.7
Boscalid 1: sclex 30 0.36 75.0 52.5 142.8
Boscalid 1: sclex 50 0.40 67.5 51.9 130.0
Result of the test shows, the composite control sclerotinia rot of colza of Boscalid and sclex, and proportioning is 30: 1-1: in the time of between 50, co-toxicity coefficient all more than 130.0, has synergistic effect; Proportioning is 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 187.7, and synergistic effect is more obvious.
The serial proportioning of table 4 Boscalid and dimethachlon is to the Toxicity Determination result of sclerotinia rot of colza
| Handle | EC 50(μg/mL) | ATI | TTI | Co-toxicity coefficient CTC |
| Boscalid | 0.23 | 100 | / | / |
| Dimethachlon | 0.26 | 88.5 | / | / |
| Boscalid 30: dimethachlon 1 | 0.17 | 135.3 | 99.6 | 135.8 |
| Boscalid 20: dimethachlon 1 | 0.16 | 143.8 | 99.5 | 144.5 |
| Boscalid 10: dimethachlon 1 | 0.12 | 191.7 | 99.0 | 193.7 |
| Boscalid 5: dimethachlon 1 | 0.1 | 230.0 | 98.1 | 234.5 |
| Boscalid 1: dimethachlon 1 | 0.07 | 328.6 | 94.2 | 348.7 |
| Boscalid 1: dimethachlon 5 | 0.11 | 209.1 | 90.4 | 231.3 |
| Boscalid 1: dimethachlon 10 | 0.12 | 191.7 | 89.5 | 214.1 |
| Boscalid 1: dimethachlon 20 | 0.13 | 176.9 | 89.0 | 198.8 |
| Boscalid 1: dimethachlon 30 | 0.18 | 127.8 | 88.8 | 143.8 |
| Boscalid 1: dimethachlon 50 | 0.19 | 121.1 | 88.7 | 136.5 |
Result of the test shows, the composite control sclerotinia rot of colza of Boscalid and dimethachlon, and proportioning is 30: 1-1: in the time of between 50, co-toxicity coefficient all more than 135.8, has synergistic effect; Proportioning is 10: 1-1: in the time of between 20, co-toxicity coefficient all is higher than 193.7, and synergistic effect is more obvious.
Bactericidal composition of the present invention can be prepared into known method and be fit to wetting powder, suspending agent or the water dispersible granules that agricultural is used.Below describe with specific embodiment, percentage is mass percent in the prescription.In the present specification active component refer to that (A) Boscalid and (B) procymidone, second mouldly become, a kind of in sclex or the dimethachlon, below repeat no more.
One, the processing of suspending agent and application example
Each component such as active component, wetting agent, dispersant, thickener, carrier and water is even by the mixed of prescription, after grinding and/or high speed shear, obtain suspending agent.
Embodiment 1:31% Boscalid procymidone suspending agent
Boscalid 30%, procymidone 1%, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 5%, bentonite 1%, glycerine 5%, water complements to 100%.
This embodiment is applied to prevent and treat gray mold of cucumber.31% Boscalid procymidone suspending agent is sprayed by 1000 times of (Boscalid valid density is 300 μ g/ml, and procymidone valid density is 10 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 89.8%, 85.2% behind the medicine.35% Boscalid suspending agent by 1000 times (Boscalid valid density is 350 μ g/ml) and 50% procymidone water dispersible granules by 2000 times (valid density is 250 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 86.1%, 84.3% and 80.4%, 76.1% behind the medicine.The synergistic effect of the composite back of Boscalid and procymidone is obvious, and the preventive effect of melon gray mold obviously is better than single agent.
The mould prestige suspending agent of embodiment 2:10% Boscalid second
Boscalid 2%, the mould prestige 8% of second, the withered powder 8% of tea, Magnesiumaluminumsilicate 1%, bentonite 1%, ethylene glycol 5%, water complements to 100%.
This embodiment is applied to prevent and treat stem rot of cucumber.The mould one-tenth suspending agent of 10% Boscalid second is sprayed by 250 times of (Boscalid valid density is 80 μ g/ml, and the mould prestige valid density of second is 320 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 96.3%, 94.7% behind the medicine.30% Boscalid suspending agent by 1000 times (valid density is 300 μ g/ml) and the mould prestige wetting powder of 40% second by 1000 times (valid density is 100 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 87.3%, 84.5% and 83.4%, 80.1% behind the medicine.The synergistic effect of the composite back of the mould prestige of Boscalid and second is obvious, and the preventive effect of stalk break obviously is better than single agent.
The mould one-tenth suspending agent of embodiment 3:30% Boscalid second
Boscalid 10%, the mould prestige 20% of second, methyl naphthalene sulfonate formaldehyde condensate 10%, xanthans 5%, white carbon 2%, glycerine 4%, water complements to 100%.
This embodiment is applied to prevent and treat cladosporium leaf and fruit mould of tomato.The mould prestige suspending agent of 30% Boscalid second is sprayed by 1000 times of (Boscalid valid density is 100 μ g/ml, and the mould prestige valid density of second is 200 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 94.3%, 91.3% behind the medicine.20% Boscalid suspending agent by 1000 times (valid density is 200 μ g/ml) and the mould prestige wetting powder of 30% second by 1000 times (valid density is 300 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 84.3%, 81.9% and 82.4%, 80.3% behind the medicine.The synergistic effect of the composite back of the mould prestige of Boscalid and second is obvious, and the preventive effect of cladosporium leaf and fruit mould of tomato obviously is better than single agent.
Two, wetting powder processing and application example
Active component, various auxiliary agent and filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, promptly get wetting powder.
Embodiment 4:51% Boscalid procymidone wetting powder
Boscalid 1%, procymidone 50%, lauryl sodium sulfate 2%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, kaolin complements to 100%.
This embodiment is applied to the tobacco stalk break.51% Boscalid procymidone is sprayed by 800 times of (Boscalid valid density is 12.5 μ g/ml, and procymidone valid density is 625 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 87.1%, 84.3% behind the medicine.20% Boscalid suspending agent by 1000 times (valid density is 200 μ g/ml) and 35% procymidone wetting powder by 500 times (valid density is 700 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 78.9%, 75.5% and 85.3%, 80.3% behind the medicine.The synergistic effect of the composite back of Boscalid and procymidone is obvious, and the preventive effect of tobacco stalk break obviously is better than single agent.
Embodiment 5:40% Boscalid dimethachlon wetting powder
Boscalid 15%, dimethachlon 25%, the withered powder 5% of tea, sodium lignin sulfonate 5%, NNO 3%, and attapulgite complements to 100%.
This embodiment is applied to sclerotinia rot of colza.40% Boscalid dimethachlon wetting powder is sprayed by 2000 times of (Boscalid valid density is 75 μ g/ml, and dimethachlon valid density is 125 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 97.2%, 95.3% behind the medicine.20% Boscalid suspending agent by 1000 times (valid density is 200 μ g/ml) and 18% dimethachlon wetting powder by 1000 times (valid density is 180 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 86.3%, 85.7% and 85.7%, 80.4% behind the medicine.The synergistic effect of the composite back of Boscalid and dimethachlon is obvious, and the preventive effect of sclerotinia rot of colza obviously is better than single agent.
Three, water dispersible granules processing and application example
Active component, dispersant, wetting agent and filler is even by the mixed of prescription, become wetting powder through comminution by gas stream, add a certain amount of water extrusion again and make material.After dry screened, obtain water dispersible granules.
Embodiment 6:85% Boscalid sclex water dispersible granules
Boscalid 30%, sclex 55%, NNO 2%, sodium lignin sulfonate 1%, lauryl sodium sulfate 2%, bentonite complements to 100%.
This embodiment is applied to prevent and treat graw mold of tomato.85% Boscalid sclex water dispersible granules is sprayed by 1600 times of (Boscalid valid density is 187.5 μ g/ml, and sclex valid density is 343.75 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 96.9%, 94.3% behind the medicine.30% Boscalid water dispersible granules is by 1000 times (valid density is 300 μ g/ml); 50% sclex water dispersible granules is by 1000 times (valid density is 500 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 87.2%, 85.3% and 85.6%, 80.4% behind the medicine.The synergistic effect of the composite back of Boscalid and sclex is obvious, and the preventive effect of graw mold of tomato obviously is better than single agent.
Embodiment 7:60% Boscalid procymidone water dispersible granules
Boscalid 20%, procymidone 40%, Negel 2%, sodium lignin sulfonate 1%, lauryl sodium sulfate 2%, kaolin complements to 100%.
This embodiment is applied to prevent and treat grey mould fruit rot of strawberry.60% Boscalid procymidone water dispersible granules is sprayed by 2000 times of (Boscalid valid density is 100 μ g/ml, and procymidone valid density is 200 μ g/ml) thin ups, and the control efficiency of 7 days and 15 days is respectively 93.2%, 91.4% behind the medicine.20% Boscalid wetting powder is by 1000 times (valid density is 200 μ g/ml); 60% procymidone water dispersible granules is by 2000 times (valid density is 300 μ g/ml); Use with quadrat method and use, the preventive effect of 7 days and 15 days is respectively 85.2%, 84.7% and 83.8%, 80.3% behind the medicine.The synergistic effect of the composite back of Boscalid and procymidone is obvious, and the preventive effect of grey mould fruit rot of strawberry obviously is better than single agent.
Claims (5)
1. bactericidal composition, it is characterized in that: contain active ingredient Boscalid and procymidone in the said bactericidal composition, its mass ratio is 30: 1-1: 50.
2. according to the said bactericidal composition of claim 1, it is characterized in that: the mass ratio of active ingredient Boscalid and procymidone is 10: 1-1: 20.
3. according to claim 1 or 2 said bactericidal compositions, it is characterized in that: the gross mass percentage composition of active ingredient in bactericidal composition is 10%-85%.
4. according to the said bactericidal composition of claim 3, it is characterized in that: the formulation of said bactericidal composition is wetting powder, suspending agent or water dispersible granules.
5. the application of bactericidal composition according to claim 1 on crop pest.
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| CN104041542A (en) * | 2014-06-25 | 2014-09-17 | 无锡市崇安区科技创业服务中心 | Medicament for controlling gray mold of alocasia macrorrhiza and application method thereof |
| CN105076140A (en) * | 2015-09-06 | 2015-11-25 | 安徽出入境检验检疫局检验检疫技术中心 | Bactericidal composition containing fluopyram and dimetachlone |
| CN113785838B (en) * | 2021-10-21 | 2022-11-22 | 浙江农林大学 | Combination fungicides containing chlorophenoxyamide and procymidone |
| CN114258916B (en) * | 2022-01-11 | 2023-07-28 | 江西禾益化工股份有限公司 | JuncJing compound boscalid suspending agent and preparation method and application thereof |
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| CN1395833A (en) * | 2002-07-04 | 2003-02-12 | 胡凡 | Disinfeeting procymidone-diethofencarb composition |
| CN101272686A (en) * | 2005-09-29 | 2008-09-24 | 詹森药业有限公司 | Synergistic imazalil combinations |
| CN101304658A (en) * | 2005-11-10 | 2008-11-12 | 巴斯夫欧洲公司 | Fungicidal mixtures comprising boscalid and pyrimethanil |
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| CN1395833A (en) * | 2002-07-04 | 2003-02-12 | 胡凡 | Disinfeeting procymidone-diethofencarb composition |
| CN101272686A (en) * | 2005-09-29 | 2008-09-24 | 詹森药业有限公司 | Synergistic imazalil combinations |
| CN101304658A (en) * | 2005-11-10 | 2008-11-12 | 巴斯夫欧洲公司 | Fungicidal mixtures comprising boscalid and pyrimethanil |
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