CN106359455A - Paecilomyces lilacinus wettable powder and preparation method thereof - Google Patents
Paecilomyces lilacinus wettable powder and preparation method thereof Download PDFInfo
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- CN106359455A CN106359455A CN201610763261.8A CN201610763261A CN106359455A CN 106359455 A CN106359455 A CN 106359455A CN 201610763261 A CN201610763261 A CN 201610763261A CN 106359455 A CN106359455 A CN 106359455A
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- paecilomyces lilacinus
- wettable powder
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
Abstract
The invention provides paecilomyces lilacinus wettable powder, prepared from the following components in parts by weight: 2-10 parts of a pharmaceutical active component, 5.4-9.5 parts of an auxiliary agent and 78.5-94.6 parts of a carrier, wherein the pharmaceutical active component is paecilomyces lilacinus. The paecilomyces lilacinus wettable powder provided by the invention is efficient, low in toxicity and stable in insecticidal effect, and is safe for people, livestock and crops, thus being high in safety. Experimental results indicate that the pest control effect of the paecilomyces lilacinus wettable powder reaches up to 81% or more.
Description
Technical field
The present invention relates to technical field of pesticide is and in particular to arrive a kind of Paecilomyces lilacinus wettable powder and preparation method.
Background technology
China is large agricultural country, and for thousands of years always based on planting industry, agricultural is the source of basic necessities of life of the mankind, survives it
This, be that other departments are able to independent and development basis, be the basis of national economy, the development of therefore agricultural will directly affect
The development of national economy.But in agricultural production, the generation of insect pest often has a strong impact on crop growth, make crops from
Physiological function there occurs a series of change to organizational structure and destroys, and then leads to a large amount of underproduction of crops, carries to grower
Carry out huge economic loss.Therefore the effective preventing and treating to crop pests is instant, to ensure grain security and kind
The economic interests of plant person.
The species of crop pests has many kinds at present, and wherein nematicide can attack and parasitic plant, by invasion and attack and parasitism
Plant affects normal growth promoter because nematicide absorbs endotrophic, and the secretions of nematode metabolism can stimulate host plant
Cell and tissue, lead to plant deformity.Most plants nematicide encroaches on the under ground portion of plant, such as root, tuber etc., and root can
Show as dross, necrosis, root is short and thick and grows thickly, lead to a large amount of underproduction of crops or quality degradation.Common Plant nematode
Disease has root knot nematode disease, soybean cyst nematode, wheat anguina disease, sweep stem nematode, aphelenchoides besseyi disease, Citrus half
Puncture nematicide etc..
In prior art, usually carry out pest control disease from disease-resistant variety or chemical insecticide.Using chemical insecticide
When being prevented and treated, although prevention effect is preferable, chemical insecticide has difficult degradation, is easily destroyed the balance of ecosystem,
People and animals are endangered larger.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide a kind of Paecilomyces lilacinus wettable
Property powder and preparation method thereof.
The invention provides a kind of Paecilomyces lilacinus wettable powder, including the component of following parts by weight:
2～10 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
5.4～9.5 parts of auxiliary agent；
78.5～94.6 parts of carrier.
Preferably, effective spore amount of described Paecilomyces lilacinus wettable powder is 0.4～2.0 × 109cfu/g.
Preferably, miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus wettable powder.
Preferably, suspensibility >=80% of described Paecilomyces lilacinus wettable powder.
Preferably, spore rate >=90% alive of the Paecilomyces lilacinus of described Paecilomyces lilacinus wettable powder.
Preferably, the wetting time≤120s of described Paecilomyces lilacinus wettable powder.
Preferably, described auxiliary agent includes fatty alcohol and ethylene oxide condensate, naphthalene sulphonate, wetting agent efw, different
One or more of octyl group sodium succinate, sodium metnylene bis-naphthalene sulfonate and sodium lauryl sulphate.
Preferably, described carrier is included in white carbon, bentonite, sodium sulfate, potter's clay, calcined diatomite and calcined kaolin
One or more.
The invention provides the preparation method of the Paecilomyces lilacinus wettable powder of technique scheme, walk including following
Rapid:
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains Paecilomyces lilacinus wettable powder.
Further, application in terms of preventing and treating crop pests for the described Paecilomyces lilacinus wettable powder.
The invention provides a kind of Paecilomyces lilacinus wettable powder, including the component of following parts by weight: pharmaceutically active
2～10 parts of component, described medicine activity component is Paecilomyces lilacinus；5.4～9.5 parts of auxiliary agent；78.5～94.6 parts of carrier.?
In the present invention, Paecilomyces lilacinus wettable powder is efficient, low toxicity, and insecticidal effect is stable, and to people and animals and crop safety, safety
High.Test result indicate that, the effect of Paecilomyces lilacinus wettable powder pest control reaches as high as more than 81%.
Biological deposits explanation
Paecilomyces lilacinus (paecilomyces lilacinus), are deposited in China Microbiological bacterium on 13rd in August in 2013
Plant preservation administration committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the Chinese Academy of Sciences
Institute of microbiology, it is cgmccno.7994 that biological deposits are numbered.
Specific embodiment
The invention provides a kind of Paecilomyces lilacinus wettable powder, including the component of following parts by weight: pharmaceutically active
2～10 parts of component, described medicine activity component is Paecilomyces lilacinus；5.4～9.5 parts of auxiliary agent；78.5～94.6 parts of carrier.
The Paecilomyces lilacinus wettable powder that the present invention provides preferably includes 2～10 parts of Paecilomyces lilacinus, and more preferably 3
～8 parts, most preferably 5 parts.
In the present invention, described Paecilomyces lilacinus are preferably selected from biological deposits and number being the strain of cgmcc no.7994
Activation culture obtains.
In the present invention, the preparation method of described Paecilomyces lilacinus preferably includes following steps:
1) Paecilomyces lilacinus strain is activated；
2) Paecilomyces lilacinus after described activation are inoculated in paecilomyces lilacinus fluid medium and carry out liquid culture,
Culture 55～75h stops culture, obtains Paecilomyces lilacinus culture fluid；
3) by described step 2) the Paecilomyces lilacinus culture fluid liquid that obtains is inoculated in Paecilomyces lilacinus solid medium, enters
Row solid culture, obtains Paecilomyces lilacinus.
In the present invention, the activation of described Paecilomyces lilacinus strain is preferably and is inoculated in the Paecilomyces lilacinus strain of preservation
Equipped with the eggplant type bottle of nutrient agar, 25～30 DEG C of culture 10～14h carry out activation culture.
After described activation culture, the Paecilomyces lilacinus after described activation are inoculated in the training of paecilomyces lilacinus liquid by the present invention
Carry out liquid culture in foster base.In the present invention, the inoculum concentration of described Paecilomyces lilacinus liquid culture is preferably 10～30%, more
It is preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus culture fluid be inoculated in solid medium inoculum concentration be preferably 10～
30%, more preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus fluid medium preferably includes following components: sucrose 20～40g/l, yellow
Semen Glycines powder 1.0～10g/l, ammonium nitrate 1.0～2.0g/l, disodium hydrogen phosphate 1.5～3.0g/l, magnesium sulfate 0.15～0.32g/l, chlorine
Change potassium 0.1～1.0g/l, iron sulfate 0.01～0.10g/l；More preferably sucrose 25～35g/l, analysis for soybean powder 3.0～7g/l, nitre
Sour ammonium 1.3～1.7g/l, disodium hydrogen phosphate 1.8～2.4g/l, magnesium sulfate 0.20～0.28g/l, potassium chloride 0.3～0.7g/l,
Iron sulfate 0.03～0.07g/l；Most preferably sucrose 30g/l, analysis for soybean powder 5g/l, ammonium nitrate 1.5g/l, disodium hydrogen phosphate
2.06g/l, magnesium sulfate 0.24g/l, potassium chloride 0.5g/l, iron sulfate 0.05g/l.The present invention does not have spy to the source of said components
The different medicine limiting, culture medium being formulated conventionally using those skilled in the art.
In the present invention, described Paecilomyces lilacinus fluid medium preferably makes after 120～130 DEG C of sterilizing 30～45min
With.In the present invention, more preferably 125 DEG C of described sterilising temp；Described sterilization time is more preferably 40min.The present invention is to training
The sterilizing installation of foster base is not particularly limited, and using sterilizing installation well known to those skilled in the art, such as high steam goes out
Bacterium pot.
The present invention does not have special restriction to the equipment of described liquid culture, using Zymolysis Equipment well known in the art
, such as fermentation tank.
In the present invention, the temperature of described Paecilomyces lilacinus liquid culture is preferably 25～30 DEG C, more preferably 26～
29 DEG C, most preferably 28 DEG C；The ph value of described Paecilomyces lilacinus liquid culture fermentation is preferably 6.6～7.2, more preferably
6.8～7.0, most preferably 6.9；The ambient pressure of described Paecilomyces lilacinus liquid culture is preferably 0.05～0.1mpa, more excellent
Choosing for 0.07～0.08mppa；The present invention is ventilated in described Paecilomyces lilacinus liquid culture, and described ventilation is preferred
For 0.8～1.2v/v min, more preferably 1v/v min；The time of described Paecilomyces lilacinus liquid fermentation be preferably 55～
75h, more preferably 60～70h, more preferably 65h.
After obtaining Paecilomyces lilacinus culture fluid, the Paecilomyces lilacinus obtaining culture fluid is inoculated in solid medium by the present invention
In, carry out solid culture, obtain Paecilomyces lilacinus.In the present invention, the inoculum concentration of described solid culture is preferably 10～30%,
More preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus solid medium preferably includes the component of following weight portion: Testa Tritici 60～
100 parts, 5～15 parts of Semen Maydis powder, 5～15 parts of rice husk；More preferably 70～90 parts of Testa Tritici, 7～12 parts of Semen Maydis powder, rice husk 7～12
Part；Most preferably 80 parts of Testa Tritici, 10 parts of Semen Maydis powder, 10 parts of rice husk.The present invention is preferably in described Paecilomyces lilacinus solid medium
Middle interpolation distilled water, makes solid medium water content be preferably 45%～55%, more preferably 47%～52%, most preferably
50%, then carry out solid fermentation.The present invention is not particularly limited to the source of said components, normal using those skilled in the art
Rule prepare the medicine of culture medium.
In the present invention, the temperature of described Paecilomyces lilacinus solid culture is preferably 24～30 DEG C, more preferably 26～
29 DEG C, most preferably 28 DEG C；The time of described Paecilomyces lilacinus solid culture is preferably 5～10d, more preferably 6～9d, optimum
Elect 7d as；First three sky of humidity of described Paecilomyces lilacinus solid culture is preferably 60%～90%, and remaining time is preferably 10%
～40%；More preferably 70%～85%, 15%～35%；Most preferably 80%, 30%.
The present invention is preferably 3～8cm, more preferably 5～7cm to the thickness of described paecilomyces fumosoroseus solid medium,
Most preferably 6cm.
After completing described Paecilomyces lilacinus solid culture, the present invention preferably by obtain the culture medium after solid culture dry,
Pulverize and sieve, obtain solid, powdery Paecilomyces lilacinus.In the present invention, the temperature of described drying is preferably 30～50 DEG C, more
It is preferably 35～45 DEG C, most preferably 40 DEG C；The time of described drying is preferably 20～45h, more preferably 30～40h, optimum
Elect 35h as.Solid fermentation culture medium is dried to moisture preferably between 25～35% by the present invention, more preferably 27～
32%, most preferably 30%.The method that the present invention pulverizes to the culture medium after described solid culture does not have special restriction, adopts
Breaking method well known to those skilled in the art.In the present invention, described Paecilomyces lilacinus sieve and preferably employ 80～100
Mesh, more preferably 85～95 mesh, most preferably 90 mesh.
In the present invention, effective spore amount of the Paecilomyces lilacinus that described solid culture obtains is 2.0 × 1010Cfu/g with
On.
The Paecilomyces lilacinus wettable powder that the present invention provides preferably includes 5.4～9.5 parts of auxiliary agents, more preferably 5.7～
8.2 parts, most preferably 7 parts.The present invention is not particularly limited to the species of described auxiliary agent and source, using those skilled in the art
Known to the auxiliary agent of agricultural wettable powder commercial goods.The species of auxiliary agent is preferably specifically fatty alcohol and epoxy
Oxide condensate, naphthalene sulphonate, wetting agent efw, iso-octyl sodium succinate, sodium metnylene bis-naphthalene sulfonate and dodecyl
One or more of sodium sulfate is more preferably fatty alcohol and ethylene oxide condensate, sodium metnylene bis-naphthalene sulfonate and dodecyl
Sodium sulfate, most preferably iso-octyl sodium succinate, naphthalene sulphonate and wetting agent efw.When described auxiliary agent be fatty alcohol with
When ethylene oxide condensate, sodium metnylene bis-naphthalene sulfonate and sodium lauryl sulphate, described fatty alcohol and ethylene oxide condensate,
The weight fraction of sodium metnylene bis-naphthalene sulfonate and sodium lauryl sulphate is than preferably (3.0～5.0): (2.0～3.0): (0.4
～1.5), more preferably (3.4～4.5): (1.5～2.5): (0.8～1.2), most preferably 4:2:1.When described auxiliary agent is different
When octyl group sodium succinate, naphthalene sulphonate and wetting agent efw, described iso-octyl sodium succinate, naphthalene sulphonate and
The ratio of weight and number of wetting agent efw is preferably (3.0～5.0): (2.0～3.0): (0.4～1.5), more preferably (3.4～
4.5): (1.5～2.5): (0.8～1.2), most preferably 4:2:1.In the present invention, described wetting agent efw is alkyl naphthalene sulfonic acid
Salt and anion humectant mixture, the present invention is not particularly limited to the source of described wetting agent efw, using art technology
Commercial goods known to personnel.In the present invention, described auxiliary agent makes medicine character stable, improves drug effect.
The Paecilomyces lilacinus wettable powder that the present invention provides preferably includes 78.5～94.6 parts of carriers, and more preferably 82.8
～92.3 parts, most preferably 88 parts.In the present invention, described carrier is preferably white carbon, bentonite, sodium sulfate, potter's clay, calcining
One or more of kieselguhr and calcined kaolin.The present invention does not have special restriction to the source of described carrier, using city
Sell commodity.In the present invention, described carrier is preferably specifically arbitrarily several in above-mentioned substance, can mix arbitrary proportion
Close.In the present invention, described carrier is specifically more preferably white carbon and the calcined diatomite in above-mentioned substance, specifically can be excellent
Elect 2.0～4.0 parts of white carbons and 76.5～90.6 parts of calcined diatomites as, more preferably 2.5～3.5 parts white carbons and 80.3～
88.8 parts of calcined diatomites, most preferably 3 parts white carbons and 85 parts of calcined diatomites；White carbon most preferably in above-mentioned substance
And calcined kaolin, it is preferably specifically 2.0～4.0 parts of white carbons and 76.5～90.6 parts of calcined kaolins, more preferably
2.5～3.5 parts of white carbons and 80.3～88.8 parts of calcined kaolins, most preferably 3 parts white carbons and 85 parts of Kaolin.The present invention
Described carrier has good medicine stability, also can improve the drug effect of medicine.
The invention provides the preparation method of Paecilomyces lilacinus wettable powder described in technique scheme, walk including following
Rapid:
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains Paecilomyces lilacinus wettable powder.
In the present invention, the mixing of described carrier, medicine activity component and auxiliary agent is preferably carrier, medicine activity component
It is divided into 3～5 parts with auxiliary agent respectively, more preferably 4 parts, after the 1st part of carrier is mixed with the 1st part of medicine activity component, then
Mix with the 1st part of auxiliary agent, then mix with the 2nd part of carrier again, then mix with the 2nd part of medicine activity component, then with the 2nd part of auxiliary agent
Mixing, by that analogy, until having added all of raw material, is further continued for preferably mixing 20～40min, more preferably 25～35min,
Most preferably 30min.The present invention does not have special restriction to the equipment of described mixing, using well known to those skilled in the art mixed
Conjunction equipment, such as mixing fill.
In the present invention, 10～45 μm are preferably to the granularity of described powder, more preferably 20～35 μm, most preferably 25
～30 μm.The present invention does not have special restriction to the condition of described pulverizing, using pulverization conditions well known to those skilled in the art
?.In the present invention, described pulverizing is specifically preferably pulverized for air-flowing type, and the sealing pressure that air-flowing type is pulverized is preferably
0.05～0.20mpa, more preferably 0.1～0.15mpa, most preferably 0.13mpa；The discharging sealing gland pressure that air-flowing type is pulverized is excellent
Elect 0.1～0.2mpa, more preferably 0.15mpa as；The pulsating pressure that air-flowing type is pulverized is preferably 0.3～0.7mpa, more preferably
For 0.4～0.6mpa, most preferably 0.5mpa；The feed pressure of air-flowing type preferably 0.7～0.9mpa, more preferably 0.75～
0.85mpa, most preferably 0.8mpa.The present invention does not have special restriction to the equipment of described pulverizing, using people in the art
Disintegrating apparatus known to member.
In the present invention, effective spore amount of described Paecilomyces lilacinus wettable powder be preferably 0.4～2.0 ×
109Cfu/g, more preferably 0.8～1.6 × 109Cfu/g, most preferably 1.0～1.2 × 109cfu/g.In the present invention, described
Spore rate >=90% alive of Paecilomyces lilacinus wettable powder；Miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus wettable powder；Institute
State suspensibility >=80% of Paecilomyces lilacinus wettable powder；Wetting time≤the 120s of described Paecilomyces lilacinus.
Paecilomyces lilacinus wettable powder of the present invention can be applicable to the aspect of crops pest control, there is provided a kind of
Application in terms of preventing and treating crop pests for the described Paecilomyces lilacinus wettable powder.Specifically, applicable crop specie
Including cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Paecilomyces lilacinus oil of the present invention suspends
Agent can prevent and treat root knot nematode disease, soybean cyst nematode, wheat anguina disease, sweep stem nematode, aphelenchoides besseyi disease, Citrus chachiensis Hort.
Fructus Citri tangerinae Tylenchulus Semipenetrans disease etc..
The present invention is not particularly limited to the application method of described Paecilomyces lilacinus wettable powder, using this area skill
Application method known to art personnel.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the reality in the present invention
Apply example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all belong to
In the scope of protection of the invention.
Embodiment 1
The preparation of Paecilomyces lilacinus: the Paecilomyces lilacinus strain of preservation is inoculated in the eggplant type equipped with nutrient agar
In bottle, 28 DEG C of culture 12h carry out activation culture；Paecilomyces lilacinus after activation culture are inoculated in 121 DEG C with 20% inoculum concentration
In fluid medium after sterilizing 35min, fermentation tank carries out liquid culture, the condition of concrete culture is: temperature is 28 DEG C；
Ph value is 6.9；Pressure inside the tank is 0.08mpa, and culture ventilation is 1v/v min；Stop culture after 65h, obtain pale purple plan blue or green
Mould culture fluid；The Paecilomyces lilacinus obtaining culture fluid is inoculated in solid medium with 20% inoculum concentration, in solid culture
Add distilled water in base, make solid medium water content be 50%, then carry out solid culture, condition of culture is: cultivation temperature
For 28 DEG C, culture first three sky of humidity is 80%, and culture humidity is 30% within latter four days, and incubation time is 7 days；After solid culture terminates
The Paecilomyces lilacinus of the solid culture obtaining are dried under the conditions of 40 DEG C 35h, dry Paecilomyces lilacinus to solid culture
Moisture is 30%, pulverized 90 mesh sieves, and obtained Paecilomyces lilacinus, and after testing, effective spore amount of Paecilomyces lilacinus is 2.0
×1010More than cfu/g.
Embodiment 2
The preparation of Paecilomyces lilacinus wettable powder: in terms of parts by weight, by 3 parts of white carbons, 85 parts of calcined kaolins, 5
The Paecilomyces lilacinus of part embodiment 1 preparation, 4 parts of iso-octyl sodium succinates, 2 parts of naphthalene sulphonate and 1 part of wetting agent efw divide
It is not divided into 4 heaps, after the 1st heap white carbon is mixed with the 1st heap calcined kaolin, then mix with the 1st heap Paecilomyces lilacinus, then
Mix with the 1st heap iso-octyl sodium succinate, then mix with the 1st heap naphthalene sulphonate, then mix with the 1st heap wetting agent efw,
Mix with the 2nd heap white carbon again, by that analogy, until all raw materials have all mixed, be further continued for mixing 30min, mixed
Material；Compound is pulverized through air-flowing type, obtains 25 μm of powder, that is, obtains Paecilomyces lilacinus wettable powder.
The quality control index of the Paecilomyces lilacinus wettable powder of table 1 embodiment 2 preparation
Embodiment 3
The preparation of Paecilomyces lilacinus wettable powder: in terms of parts by weight, by 3 parts of white carbons, 85 parts of calcined diatomites, 5
The Paecilomyces lilacinus of part embodiment 1 preparation, 4 parts of fatty alcohol and ethylene oxide condensate, 2 parts of sodium metnylene bis-naphthalene sulfonates, 1 part ten
Sodium dialkyl sulfate is divided into 4 heaps respectively, after the 1st heap white carbon is mixed with the 1st heap calcined diatomite lighter with the 1st heap
Purple Paecilomyces varioti mixing, then mix with ethylene oxide condensate with the 1st heap fatty alcohol, then mix with the 1st heap sodium metnylene bis-naphthalene sulfonate
Close, then mix with the 1st heap sodium lauryl sulphate, then mix with the 2nd heap white carbon, by that analogy, until all raw materials are whole
Mix, be further continued for mixing 20min, obtain compound；Compound is pulverized through air-flowing type, obtains 20 μm of powder, that is, obtains light
Purple Paecilomyces varioti wettable powder.
Embodiment 4
The preparation of Paecilomyces lilacinus wettable powder: in terms of parts by weight, by 3.5 parts of white carbons, 80.3 parts of calcining kaolinites
Soil, the Paecilomyces lilacinus of 8 parts of embodiment 1 preparations, 4.5 parts of iso-octyl sodium succinates, 2.5 parts of naphthalene sulphonate and 1.2 parts
Wetting agent efw is divided into 4 heaps respectively, after the 1st heap white carbon is mixed with the 1st heap calcined kaolin, the more pale purple plan with the 1st heap
Penicillium sp mixes, then mixes with the 1st heap iso-octyl sodium succinate, then mixes with the 1st heap naphthalene sulphonate, then moistens with the 1st heap
Humectant efw mixes, then mixes with the 2nd heap white carbon, by that analogy, until all raw materials have all mixed, is further continued for mixing
30min, obtains compound；Compound is pulverized through air-flowing type, obtains 20 μm of powder, that is, obtains Paecilomyces lilacinus wettable powder
Agent.
Embodiment 5
The test of pesticide effectiveness: this test is carried out in Jiangxi Province, preventing and treating kind is Fructus Lycopersici esculenti, and management is good, during root-knot nematode occurs
Deng weighting.Experimental field is divided into three regions by this test, and region 1 is matched group, a spray clear water；Region 2 sprays enforcement
2000 times of diluents of Paecilomyces lilacinus wettable powder of preparation in example 2；Region 3 sprays the 2000 times of dilutions of 50% phoxim emulsifiable concentrate
Liquid, the concentration of medicine activity component is identical with region 2；Trizonal dosage is identical, 1 day after dispenser, 3 days, and each investigation in 7 days
Once borer population alive, calculates Revision insect recluced rate.Concrete outcome as shown in table 2, can show, the insect population in region 2 subtracts by the data of table 2
Move back rate highest, show that the insecticidal effect of the Paecilomyces lilacinus wettable powder of present invention preparation is best.
Parasite killing (root-knot nematode) effect of table 2 Paecilomyces lilacinus wettable powder
Can draw from the test data of table 2, the Paecilomyces lilacinus wettable powder insect population of 7 days of present invention preparation goes down
Rate may be up to 81.6%, and its insecticidal effect is higher than 50% phoxim emulsifiable concentrate.
As seen from the above embodiment, the Paecilomyces lilacinus wettable powder of present invention preparation, the control rate to root-knot nematode
May be up to more than 81%, insecticidal effect is higher than effective cypermethrin.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Paecilomyces lilacinus wettable powder, including the component of following parts by weight:
2～10 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
5.4～9.5 parts of auxiliary agent；
78.5～94.6 parts of carrier.
2. Paecilomyces lilacinus wettable powder according to claim 1 is it is characterised in that described Paecilomyces lilacinus wettability
Effective spore amount of powder is 0.4～2.0 × 109cfu/g.
3. the Paecilomyces lilacinus wettable powder according to claim 1～2 any one is it is characterised in that described is light
Miscellaneous bacteria rate≤0.1% of purple Paecilomyces varioti wettable powder.
4. Paecilomyces lilacinus wettable powder according to claim 2 is it is characterised in that described Paecilomyces lilacinus wettability
Suspensibility >=80% of powder.
5. the Paecilomyces lilacinus wettable powder according to claim 2 or 4 is it is characterised in that described Paecilomyces lilacinus can
Spore rate >=90% alive of the Paecilomyces lilacinus of WP.
6. Paecilomyces lilacinus wettable powder according to claim 2 is it is characterised in that described Paecilomyces lilacinus wettability
Wetting time≤the 120s of powder.
7. Paecilomyces lilacinus wettable powder according to claim 1 it is characterised in that described auxiliary agent include fatty alcohol with
Ethylene oxide condensate, naphthalene sulphonate, wetting agent efw, iso-octyl sodium succinate, sodium metnylene bis-naphthalene sulfonate and 12
One or more of alkyl sodium sulfate.
8. the Paecilomyces lilacinus wettable powder according to claim 1 or 6 is it is characterised in that described carrier includes Linesless charcoal
One or more of black, bentonite, sodium sulfate, potter's clay, calcined diatomite and calcined kaolin.
9. the preparation method of the Paecilomyces lilacinus wettable powder described in claim 1～8 any one, comprises the following steps:
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains Paecilomyces lilacinus wettable powder.
10. the Paecilomyces lilacinus wettable powder described in claim 1～8 any one or the method system described in claim 9
Application in terms of preventing and treating crop pests for the standby Paecilomyces lilacinus wettable powder obtaining.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN110628651A (en) * | 2019-10-21 | 2019-12-31 | 昆明理工大学 | Liquid submerged fermentation method for improving activity of paecilomyces lilacinus spores |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322564A (en) * | 2014-10-11 | 2015-02-04 | 河北百奥生物制品有限公司 | Trichoderma chlamydospore wettable powder composition and preparing method thereof |
| CN104365592A (en) * | 2013-08-16 | 2015-02-25 | 上海万力华生物科技有限公司 | Trichoderma wettable powder and preparation method thereof |
-
2016
- 2016-08-29 CN CN201610763261.8A patent/CN106359455A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365592A (en) * | 2013-08-16 | 2015-02-25 | 上海万力华生物科技有限公司 | Trichoderma wettable powder and preparation method thereof |
| CN104322564A (en) * | 2014-10-11 | 2015-02-04 | 河北百奥生物制品有限公司 | Trichoderma chlamydospore wettable powder composition and preparing method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 肖顺: "淡紫拟青霉对根节线虫的防治作用", 《福建农林大学学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN110628651A (en) * | 2019-10-21 | 2019-12-31 | 昆明理工大学 | Liquid submerged fermentation method for improving activity of paecilomyces lilacinus spores |
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Application publication date: 20170201 |