CN106376599A - Dry suspending agent of paecilomyces lilacinus and preparation method thereof - Google Patents
Dry suspending agent of paecilomyces lilacinus and preparation method thereof Download PDFInfo
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- CN106376599A CN106376599A CN201610752046.8A CN201610752046A CN106376599A CN 106376599 A CN106376599 A CN 106376599A CN 201610752046 A CN201610752046 A CN 201610752046A CN 106376599 A CN106376599 A CN 106376599A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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Abstract
The invention provides a dry suspending agent of paecilomyces lilacinus. The dry suspending agent comprises an active ingredient, adjuvants and auxiliary agents. The active ingredient is paecilomyces lilacinus. Spore content of paecilomyces lilacinus in the dry suspending agent is 100-50,000 million/g. The dry suspending agent of paecilomyces lilacinus has long storage time, and living cell concentration loss is less by the preparation method. The product is efficient and low-toxic, has stable insecticidal effect, is safe to human, livestock and crops, and has high safety. It shows through experimental results that nematode control effect of paecilomyces lilacinus can reach 84.7% and above.
Description
Technical field
The present invention relates to crop pests Prevention Technique field and in particular to a kind of dry suspending agent of Paecilomyces lilacinus and its
Preparation method.
Background technology
China, as traditional agriculture big country, generally adopts Agro-chemicals control disease during production estimation, though really
Keeping normal life activities produce good harvest, but the use due to chemical pesticide, work the mischief often to environment and the mankind.Theory is managed in environmental protection in recent years
Increasingly become mainstream of society theory, and government it is also proposed double policies and measures subtracting, thus, develop safe and environment-friendly and
Efficient green pesticide has been instant responsibility.
Paecilomyces lilacinus belong to endoparasitism funguses, are the Important Natural Enemies of some plant nematodes, can parasitize ovum,
Also larva and female adult can be infected, can substantially mitigate the danger of the nemases such as various crop root-knot nematode, Cyst nematode, Ditylenchus dipsaci
Evil.It is in ampuliform or subsphaeroidal (bottle stalk) that this genus is mainly characterized by conidiophore, verticillate on mycelia end or brachyplast, conidium
Monospore chain, so far this genus report have nearly 50 kinds, be all insect pathogenic bacteria and nematicide pathogen.This bacterium is distributed widely in the world
Various places, have the advantages that effect height, Ji Zhuguang, easily cultivate, especially effect is distinguished in terms of controlling plant pathogeny line insect.More than half
Since century, many experts and scholars have made extensive and intensive studies to this bacterium both at home and abroad, in biology, ecology, a large amount of
The aspects such as culture, pest controlling efficiency and Field information achieve a series of achievements in research.
Paecilomyces lilacinus dosage form is mainly with conventional dosage forms such as suspending agent agent, wettable powders in the market.But water preparation exists
Easy layering and precipitating in storage, and be easy to inactivate, it is difficult to promote preserving；Wettable powder is also easy to produce powder when producing and applying
, " caking " when disperseing in water in dirt, and before and after causing spray because of sedimentation during spraying, concentration differs, and these are all seriously made
About the promotion and application of Paecilomyces lilacinus insecticide.
Content of the invention
In view of this, it is an object of the invention to provide a kind of Paecilomyces lilacinus dry suspending agent, viable count is high, period of storage
Long.And provide the preparation method of dry suspending agent, the loss of Paecilomyces lilacinus when can reduce preparation.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of Paecilomyces lilacinus dry suspending agent, including effective ingredient, auxiliary agent and adjuvant；
Described effective ingredient be Paecilomyces lilacinus, the Paecilomyces lilacinus spore content in described dry suspending agent be 1.0～
500.0 hundred million/gram.
Preferably, described adjuvant is selected from sucrose, glucose, analysis for soybean powder, rice meal, wheat flour, corn starch, Rhizoma Solani tuber osi shallow lake
One or more of powder, Testa Tritici, vitamin C, citric acid and salicylic acid.
Preferably, described auxiliary agent is selected from polymerization of carboxylic acid salt, naphthalene sulfonate, fatty alcohol-polyoxyethylene ether, alkyl phenol polyoxy second
One of alkene ether, phenethyl phenol polyethenoxy ether, iso-octyl succinate, sulfate, dibasic alkaliine and nitrate or many
Kind.
Preferably, the mass ratio of described adjuvant and auxiliary agent is (30～65)：(5～15).
Preferably, the gross mass of described auxiliary agent and adjuvant and the mass ratio of effective ingredient are (50～99):(1～50).
It is furthermore preferred that the gross mass of described auxiliary agent and adjuvant is (58～90) with the mass ratio of effective ingredient:(10～42).
Further, the preparation method of described dry suspending agent, comprises the following steps：
A, Paecilomyces lilacinus are carried out solid fermentation, the solid medium obtaining is mixed with water, obtain after described fermentation
Fermentation slurry；
B, auxiliary agent and adjuvant are mixed and pulverize after, mix with the fermentation slurry obtaining in described step a, obtain dry suspension
Mixing slurry；
C, the dry suspension mixed solution slurry obtaining described b step are spray-dried, and obtain Paecilomyces lilacinus dry suspending agent.
Preferably, the particle diameter D of mixture after pulverizing in described step b95≤ 10 microns.
Preferably, the inlet temperature being spray-dried in described step c：90～110 DEG C, the outlet temperature of described spray drying
Degree：80～90 DEG C.
It is furthermore preferred that described hothouse inlet temperature：95 DEG C, hothouse outlet temperature：80℃.
Further, application in preventing and treating crop nematodiasis for the described dry suspending agent.
Compared with existing product and its technology of preparing, the present invention has advantages below to the present invention：
Because dry suspending agent needs to obtain product through spray drying treatment, in dry run, material can be subject to height
The test of temperature, dry suspending agent needs certain thermostability, and otherwise dried product suspensibility can be because being heated particle aggregation
Decline, so adjuvant selected by dry suspending agent, auxiliary agent kind and consumption are particularly important.The Paecilomyces lilacinus providing in the present invention
Selection of auxiliary polymerization of carboxylic acid salt, naphthalene sulfonate, fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, benzene second in dry suspending agent
One or more of base phenol polyethenoxy ether, iso-octyl succinate, sulfate, dibasic alkaliine and nitrate, act primarily as
Moistening and peptizaiton, in conjunction with act primarily as load and diluting effect adjuvant, the two with the use of and configuration proportion, energy
Enough provide heat resistance for dry suspending agent, obtain the good dry suspending agent of particle aggregation performance.Therefore, the pale purple plan that the present invention provides
Penicillium sp dispersive property is good.
The Paecilomyces lilacinus dry suspending agent that the present invention provides is used cooperatively with auxiliary agent by adjuvant, plays synergism, has
The reduction delaying active substance Paecilomyces lilacinus spore content during preparation stored of effect, thus extend the preservation of dry suspending agent
Time and effect duration.
It is the pale purple of 1.0～500.0 hundred million/gram that the Paecilomyces lilacinus dry suspending agent that the present invention provides can load viable bacteria amount
Paecilomyces varioti, can improve the practicality of dry suspending agent with the requirement of different active substance contents in actually used for the flexible adaptation.
Compared with traditional formulations of pesticide, dry suspending agent has the advantages that solid preparation and liquid preparation to dry suspending agent,
In water, energy fater disintegration, has preferable dispersibility and suspension；The granule of its active ingredient is thin, and the medicament of unit mass is being made
The broad covered area on thing surface, has preferable drug effect；Non-dusting during production and application, reduces to environment and operator
Harm, and can make the product of high concentration, reduce storing cost it is easy to popularization and application.
Therefore, the Paecilomyces lilacinus dry suspending agent that the present invention provides solves existing Paecilomyces lilacinus preparation stored layered
Precipitation, when the time is long, Paecilomyces lilacinus lose activity, dust pollution during administration, the problems such as before and after spray, concentration differs.
The present invention provides the preparation method of Paecilomyces lilacinus dry suspending agent chamber inlet temperature to be dried by what adjustment was spray-dried
Degree, outlet temperature, in combination with the protective effect to microorganism of adjuvant and auxiliary agent, effectively reduce pale purple plan during spray drying
Penicillium sp is lost due to the bacterium number alive being affected and leading to by factors such as high temperature, moisture evaporations.Therefore, the preparation side that the present invention provides
Method can reduce the viable count loss in the preparation of Paecilomyces lilacinus dry suspending agent.
The preparation technology of the present invention compares other prillings and is easily achieved continuous prodution.
Biological deposits explanation
Paecilomyces lilacinus (Paecilomyces lilacinus), are deposited in China Committee for Culture Collection of Microorganisms
Common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, preservation
Date is August in 2013 13, and it is CGMCC No.7994 that biological deposits are numbered.
Specific embodiment
The invention provides a kind of Paecilomyces lilacinus dry suspending agent, including effective ingredient, auxiliary agent and adjuvant；
Described effective ingredient be Paecilomyces lilacinus, the Paecilomyces lilacinus spore content in described dry suspending agent be 1.0～
500.0 hundred million/gram.Preferably viable bacteria amount is 50.0～350.0 hundred million/gram, and preferred viable bacteria amount is 100.0～280.0 hundred million
Individual/gram.
In the present invention, described Paecilomyces lilacinus are preferably selected from biological deposits and number being the strain of CGMCC No.7994
Activation culture obtains.
In the present invention, the preparation method of described Paecilomyces lilacinus comprises the following steps：
1) Paecilomyces lilacinus strain is activated；
2) Paecilomyces lilacinus after described activation are inoculated in paecilomyces lilacinus basal medium and carry out liquid culture,
Culture 25～35h stops fermentation, obtains Paecilomyces lilacinus primary seed solution；
3) by described step 2) the Paecilomyces lilacinus first order seed fluid strain that obtains is in Paecilomyces lilacinus fluid medium
Carry out secondary liquid fermentation culture, culture 35～45h stops fermentation, obtains Paecilomyces lilacinus secondary seed solution；
4) by described step 3) the Paecilomyces lilacinus secondary seed solution that obtains is inoculated in solid medium and carries out solid training
Support, obtain Paecilomyces lilacinus.
In the present invention, the activation of described Paecilomyces lilacinus strain is that the Paecilomyces lilacinus strain of preservation is inoculated in equipped with battalion
In the eggplant type bottle of foster agar culture medium, 25～30 DEG C of culture 10～14h carry out activation culture.
After described activation, the Paecilomyces lilacinus after described activation are inoculated in basal medium and carry out liquid training by the present invention
Support.In the present invention, the inoculum concentration of described liquid is preferably 10～30%, more preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus basal medium preferably includes following components：Rhizoma Solani tuber osi 180～220g/
L, glucose 15～30g/L, sodium nitrate 3～7g/L, dipotassium hydrogen phosphate 0.1～1g/L, magnesium sulfate 0.5～1.5g/L, iron sulfate
0.04～0.08g/L；More preferably：Rhizoma Solani tuber osi 180g/L, glucose 25g/L, sodium nitrate 5g/L, dipotassium hydrogen phosphate 5g/L, sulfur
Sour magnesium 1g/L, iron sulfate 0.06g/L.
In the present invention, use after described Paecilomyces lilacinus basal medium is preferably sterilized；Described sterilizing is preferably height
Temperature sterilizing, the temperature of described sterilizing is preferably 120～130 DEG C, more preferably 125 DEG C；The time of described sterilizing be preferably 30～
45min, more preferably 40min.The present invention is not particularly limited to the equipment of described sterilizing, is known using those skilled in the art
Sterilizing installation, such as high-pressure steam sterilizing pan.
The present invention does not have special restriction to the equipment of described liquid culture, using culture device well known in the art
, such as shaking flask.
In the present invention, the temperature of described Paecilomyces lilacinus basic culture solution is preferably 25～30 DEG C, and more preferably 26
～29 DEG C, most preferably 27 DEG C；The pH value of described Paecilomyces lilacinus liquid culture is preferably 6.6～7.2, more preferably 6.8
～7.0, most preferably 6.9；The illumination of described Paecilomyces lilacinus liquid culture is preferably 1000～2000Lx, more preferably
1700Lx；The ambient pressure of described Paecilomyces lilacinus liquid culture preferably 0.05～0.15MPa, more preferably 0.08～
0.12MPa, most preferably 0.10MPa；The present invention is ventilated in described Paecilomyces lilacinus liquid culture, and ventilation is entered simultaneously
Row stirring, described ventilation is preferably 0.8～1.2V/V min, more preferably 1V/V min；Described Paecilomyces lilacinus liquid
The time of culture is preferably 25～35h, more preferably 28～32h, more preferably 30h.
After obtaining Paecilomyces lilacinus primary seed solution, described Paecilomyces lilacinus primary seed solution is inoculated in liquid by the present invention
Carry out second order fermentation in fermentation medium, obtain Paecilomyces lilacinus secondary seed solution.In the present invention, described primary seed solution
Inoculum concentration is preferably 10～30%, more preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus fluid medium preferably includes following components：Sucrose 200～400g/L,
Ammonium nitrate 5～30g/L, disodium hydrogen phosphate 10～40g/L, magnesium sulfate 0.1～3.5g/L, iron sulfate 0.1～1.5g/L, potassium chloride
2～6g/L；More preferably sucrose 250～350g/L, ammonium nitrate 10～20g/L, disodium hydrogen phosphate 15～25g/L, magnesium sulfate 1.5
～3g/L, iron sulfate 0.3～0.8g/L, potassium chloride 4～6g/L；Most preferably sucrose sucrose 300g/L, ammonium nitrate 15g/L, phosphorus
Sour disodium hydrogen 20g/L, magnesium sulfate 2.5g/L, iron sulfate 0.5g/L, potassium chloride 5g/L.
In the present invention, use after described Paecilomyces lilacinus fluid medium is preferably sterilized；Described sterilizing is preferably height
Temperature sterilizing, the temperature of described sterilizing is preferably 120～130 DEG C, more preferably 125 DEG C；The time of described sterilizing be preferably 30～
45min, more preferably 40min.The present invention is not particularly limited to the equipment of described sterilizing, is known using those skilled in the art
Sterilizing installation, such as high-pressure steam sterilizing pan.
The present invention does not have special restriction to the equipment of described liquid culture, using culture device well known in the art
, such as fermentation tank, shaking flask.
In the present invention, the temperature of described Paecilomyces lilacinus liquid culture is preferably 25～30 DEG C, more preferably 26～
29 DEG C, most preferably 27 DEG C；The pH value of described Paecilomyces lilacinus liquid culture be preferably 6.6～7.2, more preferably 6.8～
7.0, most preferably 6.9；The illumination of described Paecilomyces lilacinus liquid culture is preferably 1000～2000Lx, more preferably
1700Lx；The ambient pressure of described Paecilomyces lilacinus liquid culture preferably 0.05～0.15MPa, more preferably 0.08～
0.12MPa, most preferably 0.10MPa；The present invention is ventilated in described Paecilomyces lilacinus liquid culture, and ventilation is entered simultaneously
Row stirring, described ventilation is preferably 0.8～1.2V/V min, more preferably 1V/V min；Described Paecilomyces lilacinus liquid
The time of culture is preferably 35～45h, more preferably 39～44h, more preferably 42h.
Stop fermentation when Paecilomyces lilacinus fluid medium total viable count reaches 50,000,000,000/gram, obtain Paecilomyces lilacinus two
Level seed liquor, described Paecilomyces lilacinus secondary seed solution is inoculated in Paecilomyces lilacinus solid medium, carries out solid culture,
Obtain Paecilomyces lilacinus.In the present invention, the inoculum concentration of described solid culture be preferably 10～30%, more preferably 15～
25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus solid medium preferably includes the component of following weight portion：Testa Tritici 60～
100 parts, 5～15 parts of Semen Maydis powder, 5～15 parts of rice husk；More preferably 70～90 parts of Testa Tritici, 7～12 parts of Semen Maydis powder, rice husk 7～12
Part；Most preferably 80 parts of Testa Tritici, 10 parts of Semen Maydis powder, 10 parts of rice husk.The present invention is preferably in described Paecilomyces lilacinus solid medium
Middle interpolation distilled water, makes solid medium water content be preferably 45%～55%, more preferably 47%～52%, most preferably
50%, then carry out solid culture.The present invention does not have special restriction to the distilled water of described interpolation, using people in the art
The conventional use of distilled water of member.
In the present invention, the temperature of described Paecilomyces lilacinus solid culture is preferably 24～30 DEG C, more preferably 26～
29 DEG C, most preferably 28 DEG C；The time of described Paecilomyces lilacinus solid culture is preferably 5～10d, more preferably 6～9d, optimum
Elect 7d as；First three sky of humidity of described Paecilomyces lilacinus solid culture is preferably 60%～90%, and remaining time is preferably 10%
～40%；More preferably 70%～85%, 15%～35%；Most preferably 80%, 30%.
The present invention is preferably 3～8cm, more preferably 6～8cm to the thickness of described Paecilomyces lilacinus solid medium,
It is preferably 7cm.
In the present invention, in the solid medium after described fermentation, Paecilomyces lilacinus spore content is more than 20,000,000,000/gram.
In the present invention, described adjuvant is selected from sucrose, glucose, analysis for soybean powder, rice meal, wheat flour, corn starch, Ma Ling
One or more of sweet potato starch, Testa Tritici, vitamin C, citric acid and salicylic acid.The present invention does not have to the source of described adjuvant
Special restriction, using commercial goods.Specifically preferably one of sucrose and analysis for soybean powder or two kinds.In the present invention
In, described adjuvant is the carrier of dry suspending agent, plays load and the effect of dilution pesticide.
In the present invention, described auxiliary agent gathers selected from polymerization of carboxylic acid salt, naphthalene sulfonate, fatty alcohol-polyoxyethylene ether, alkyl phenol
One of oxygen vinyl Ether, phenethyl phenol polyethenoxy ether, iso-octyl succinate, sulfate, dibasic alkaliine and nitrate
Or it is multiple.The present invention does not have special restriction to the source of described auxiliary agent, using commercial goods.It is preferably specifically
One or more of ammonium sulfate, dipotassium hydrogen phosphate, zinc sulfate, iron sulfate, polymerization of carboxylic acid salt and naphthalene sulfonate.Of the present invention
Auxiliary agent be dry suspending agent wetting dispersing agent, rise wetting action and peptizaiton.
In the present invention, auxiliary agent and the mass ratio of adjuvant are (30～65)：(5～15), be preferably specifically (40～60)：
(11～14), more preferably 60:12.79.In the present invention, described auxiliary agent is compounded with adjuvant and has synergistic function, can
When delaying to store, the reduction of bacterium number alive, thus extending the shelf-life of Paecilomyces lilacinus dry suspending agent, plays good drug substance stable
Effect.
In the present invention, the gross mass of described auxiliary agent and adjuvant and the mass ratio of effective ingredient are (50～99):(1～
50), it is preferably specifically (58～90):(10～42).
The invention provides the preparation method of Paecilomyces lilacinus dry suspending agent described in technique scheme, walk including following
Suddenly：
A, Paecilomyces lilacinus are fermented after the solid medium that obtains mix with water, obtain slurry of fermenting；
B, auxiliary agent and adjuvant are mixed and pulverizes；The fermentation slurry obtaining in the material crushing and described step a is taken to mix
Close, and mix with appropriate water, stir, obtain dry suspension mixed solution slurry；
C, the dry suspension mixed solution slurry obtaining described b step are spray-dried, and obtain Paecilomyces lilacinus dry suspending agent.
In the present invention, the Paecilomyces lilacinus viable spore concentration of described fermentation slurry is 50～120,000,000,000/gram, excellent
The concentration of choosing is 50～50,000,000,000/gram.
In the present invention, there is no special restriction to the condition of described pulverizing, using powder well known to those skilled in the art
Fringe part.In the present invention, described pulverizing is specifically preferably pulverized for air-flowing type, the sealing pressure that air-flowing type is pulverized
It is preferably 0.05～0.20MPa, more preferably 0.1～0.15MPa, most preferably 0.13MPa；The discharging sealing gland that air-flowing type is pulverized
Pressure is preferably 0.1～0.2MPa, more preferably 0.15MPa；The pulsating pressure that air-flowing type is pulverized is preferably 0.3～0.7MPa,
More preferably 0.4～0.6MPa, most preferably 0.5MPa；The feed pressure of air-flowing type is preferably 0.7～0.9MPa, more preferably
0.75～0.85MPa, most preferably 0.8MPa.The present invention does not have special restriction to the equipment of described pulverizing, using this area
Disintegrating apparatus known to technical staff.
In the present invention, so that grain diameter D after described auxiliary agent and adjuvant are pulverized95≤ 10 microns, preferred grain diameter
D95≤ 8 microns.
After obtaining dry suspension mixed solution slurry, described mixing slurry is spray-dried by the present invention, obtains Paecilomyces lilacinus
Dry suspending agent.In the present invention, the inlet temperature of described spray drying：90～110 DEG C, hothouse outlet temperature：80～90 DEG C.Tool
Body preferred hothouse inlet temperature is 95 DEG C, and hothouse outlet temperature is 80 DEG C.The present invention does not have to the equipment of described pelletize
Special restriction, using Granulation Equipments well known to those skilled in the art.The condition that offer is provided of the present invention
The loss of viable count in preparation process can be effectively reduced.
Present invention also offers the Paecilomyces lilacinus dry suspending agent described in technique scheme or technique scheme institute
Application in terms of crops prevent and treat nematicide for the Paecilomyces lilacinus dry suspending agent that the method for stating prepares.Specifically, applicable
Crop specie includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Of the present invention pale purple
Paecilomyces varioti dry suspending agent can prevent and treat each class pest and evil mite, including Meloidogyne incognita, cyst roundworm, white ball cyst roundworm,
Puncture nematicide, hairworm, golden nematode, Margarita nematicide, aphid, wood louse etc..
The present invention is not particularly limited to the application method of described Paecilomyces lilacinus dry suspending agent, using art technology
Application method known to personnel.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the reality in the present invention
Apply example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all belong to
In the scope of protection of the invention.
Embodiment 1
The preparation of Paecilomyces lilacinus fermentation slurry：The Paecilomyces lilacinus strain of preservation is inoculated in equipped with Nutrient agar culture
In the eggplant type bottle of base, 28 DEG C of culture 12h carry out activation culture；Paecilomyces lilacinus after activation culture are inoculated with 20% inoculum concentration
In the basal medium after 121 DEG C of sterilizing 35min, shaking flask carries out liquid culture, the condition of concrete culture is：Temperature is
27℃；PH value is 6.9；Pressure inside the tank is 0.10MPa, and culture ventilation is 1V/V min；Illumination 1700Lx；Stop training after 30h
Support, obtain Paecilomyces lilacinus primary seed solution；Paecilomyces lilacinus primary seed solution is inoculated in 121 DEG C of sterilizings by 20% inoculum concentration
In fluid medium after 35min, fermentation tank carries out liquid culture, the condition of concrete culture is：Temperature is 27 DEG C；PH value
For 6.9；Pressure inside the tank is 0.10MPa, and culture ventilation is 1V/V min；Illumination 1700Lx；Stop culture after 42h；To obtain
Paecilomyces lilacinus secondary seed solution mixed with solid medium with 20% inoculum concentration, in solid medium add distillation
Water, makes solid medium water content be 50%, then carries out solid culture, condition of culture is：Cultivation temperature is 28 DEG C, and culture is wet
Spending first three sky is 80%, and culture humidity is 30% within latter four days, and incubation time is 7 days；Solid culture terminates to add in backward culture medium
Enter appropriate water, stir, obtain Paecilomyces lilacinus fermentation slurry.
Embodiment 2
Take 10.50kg glucose and 4.50kg aliphatic acid polyethenoxy ether AEO-5, mix homogeneously comminution by gas stream so that
Grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 1.00kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 80,000,000,000/gram, and the adjuvant crushing, agent mixture are mixed with fermentation slurry, after stirring,
Obtain the dry suspending agent wet feed that viable bacteria amount is 5,000,000,000/gram.Above-mentioned dry suspending agent wet feed, operation are processed using spray drying method
Parameter is：Hothouse inlet temperature：95℃；Hothouse outlet temperature：80℃；With this understanding, the present embodiment obtains spray dried
In dry rear product, viable count reaches 55.9 hundred million/gram.
Embodiment 3
Take 10.50kg glucose and 4.50kg aliphatic acid polyethenoxy ether AEO-5, mix homogeneously comminution by gas stream so that
Grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 1.00kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 80,000,000,000/gram, and the adjuvant crushing, agent mixture are mixed with fermentation slurry, after stirring,
Obtain the dry suspending agent wet feed that viable bacteria amount is 5,000,000,000/gram.Above-mentioned dry suspending agent wet feed, operation are processed using spray drying method
Parameter is：Hothouse inlet temperature：100℃；Hothouse outlet temperature：90℃；With this understanding, the present embodiment obtains spray dried
In dry rear product, viable count reaches 46.4 hundred million/gram.
Embodiment 4
Take 10.50kg glucose and 4.50kg aliphatic acid polyethenoxy ether AEO-5, mix homogeneously comminution by gas stream so that
Grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 1.00kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 80,000,000,000/gram, and the adjuvant crushing, agent mixture are mixed with fermentation slurry, after stirring,
Obtain the dry suspending agent wet feed that viable bacteria amount is 5,000,000,000/gram.Above-mentioned dry suspending agent wet feed, operation are processed using spray drying method
Parameter is：Hothouse inlet temperature：90℃；Hothouse outlet temperature：80℃；With this understanding, the present embodiment obtains spray dried
In dry rear product, viable count reaches 50.6 hundred million/gram.
Embodiment 5
Take 10.50kg glucose and 4.50kg aliphatic acid polyethenoxy ether AEO-5, mix homogeneously comminution by gas stream so that
Grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 1.00kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 80,000,000,000/gram, and the adjuvant crushing, agent mixture are mixed with fermentation slurry, after stirring,
Obtain the dry suspending agent wet feed that viable bacteria amount is 5,000,000,000/gram.Above-mentioned dry suspending agent wet feed, operation are processed using spray drying method
Parameter is：Hothouse inlet temperature：100℃；Hothouse outlet temperature：85℃；With this understanding, the present embodiment obtains spray dried
In dry rear product, viable count reaches 51.0 hundred million/gram.
Embodiment 6
Take 10.50kg glucose and 4.50kg aliphatic acid polyethenoxy ether AEO-5, mix homogeneously comminution by gas stream so that
Grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 1.00kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 80,000,000,000/gram, and the adjuvant crushing, agent mixture are mixed with fermentation slurry, after stirring,
Obtain the dry suspending agent wet feed that viable bacteria amount is 5,000,000,000/gram.Above-mentioned dry suspending agent wet feed, operation are processed using spray drying method
Parameter is：Hothouse inlet temperature：110℃；Hothouse outlet temperature：90℃；With this understanding, the present embodiment obtains spray dried
In dry rear product, viable count reaches 49.7 hundred million/gram.
According to embodiment 2～6, in the case of other conditions identical, optimal spray drying condition is：Hothouse enters
95 DEG C of temperature of mouth, 80 DEG C of hothouse outlet temperature, the dry suspending agent viable count highest preparing with this understanding.
Embodiment 7
Take 7.60kg polymerization of carboxylic acid salt 550S, 60.90kg sucrose and 30.50kg analysis for soybean powder, mix homogeneously comminution by gas stream,
Make grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 2kg that Example 1 preparation method obtains, pale purple plan is blue or green
Mould viable spore concentration is 5,000,000,000/gram, the adjuvant crushing, agent mixture is mixed with fermentation slurry, adds 90kg water,
After stirring, that is, obtain the dry suspending agent wet feed that viable bacteria amount is 100,000,000/gram.Above-mentioned dry suspension is processed using spray drying method
Agent wet feed, operating parameter is：Hothouse inlet temperature：95℃；Hothouse outlet temperature：80℃；With this understanding, the present embodiment
Obtain in product after being spray-dried, viable count reaches 0.98 hundred million/gram.
Embodiment 8
Take 49.50kg sucrose, 24.71kg analysis for soybean powder, 6.18kg ammonium sulfate, 1.85kg dipotassium hydrogen phosphate, 0.30kg sulphuric acid
Zinc, 0.06kg iron sulfate, 3.7kg polymerization of carboxylic acid salt 550S and 3.7kg naphthalene sulfonate D-425, mix homogeneously comminution by gas stream, make
Obtain grain diameter D95≤ 10 microns；Paecilomyces lilacinus fermentation slurry 20kg that Example 1 preparation method obtains, Paecilomyces lilacinus
Viable spore concentration is 20,000,000,000/gram, the adjuvant crushing, agent mixture is mixed with fermentation slurry, adds 90kg water,
After stirring, that is, obtain the dry suspending agent wet feed that viable bacteria amount is 2,000,000,000/gram.Above-mentioned dry suspension is processed using spray drying method
Agent wet feed, operating parameter is：Hothouse inlet temperature：95℃；Hothouse outlet temperature：80℃；With this understanding, the present embodiment
Obtain in product after being spray-dried, viable count reaches 20.3 hundred million/gram.
Embodiment 9
Take 32.49kg glucose, 16.24kg rice meal, 4.06kg sodium nitrate, 1.22kg dipotassium hydrogen phosphate, 0.19kg sulfur
Sour zinc, 0.04kg ferric nitrate and 4.06kg polymerization of carboxylic acid salt 550S, mix homogeneously comminution by gas stream are so that grain diameter D95≤10
Micron；Paecilomyces lilacinus fermentation slurry 41.70kg that Example 1 preparation method obtains, Paecilomyces lilacinus viable spore concentration
For 120,000,000,000/gram, the adjuvant crushing, agent mixture are mixed with fermentation slurry, adds 7.64kg water, stir
Afterwards, that is, obtain the dry suspending agent wet feed that viable bacteria amount is 50,000,000,000/gram.Above-mentioned dry suspending agent wet feed is processed using spray drying method,
Operating parameter is：Hothouse inlet temperature：95℃；Hothouse outlet temperature：80℃；With this understanding, the present embodiment obtains spray
In mist dried product exhibited, viable count reaches 500.0 hundred million/gram.
Embodiment 10
Take 20.33kg glucose, 30.49kg corn starch, 5.08kg dipotassium hydrogen phosphate, 1.53kg zinc sulfate, 0.30kg
Iron sulfate, 0.15kg naphthalene sulfonate D-425,2.04kg fatty alcohol-polyoxyethylene ether AEO-5 and 5.08kg phenethyl phenol polyoxy second
Alkene ether 603, mix homogeneously comminution by gas stream are so that grain diameter D95≤ 10 microns；It is pale purple that Example 1 preparation method obtains
Paecilomyces varioti fermentation slurry 35.00kg, Paecilomyces lilacinus viable spore concentration is 120,000,000,000/gram, by the adjuvant crushing, auxiliary agent
Mixture is mixed with fermentation slurry, adds 25.00kg water, and after stirring, that is, obtaining viable bacteria amount is the dry outstanding of 15,000,000,000/gram
Floating agent wet feed.Above-mentioned dry suspending agent wet feed is processed using spray drying method, operating parameter is：Hothouse inlet temperature：95℃；Dry
Dry room outlet temperature：80℃；With this understanding, after the present embodiment obtains and is spray-dried in product, viable count reach 149.7 hundred million/
Gram.
Embodiment 11
Accelerated stability test：Take adjuvant shown in 1～23 in table 1 and the common 90kg of auxiliary agent ratio, mix homogeneously gas respectively
Stream is pulverized so that grain diameter D95≤ 10 microns；The Paecilomyces lilacinus fermentation slurry that Example 1 preparation method obtains
10.00kg, Paecilomyces lilacinus viable spore concentration is 120,000,000,000/gram, sends out by auxiliary agent, the gross mass of adjuvant and Paecilomyces lilacinus
The mass ratio of zymotic fluid is 90:10 mixing, after stirring, that is, obtain the dry suspending agent wet feed that viable bacteria amount is 12,000,000,000/gram.Adopt
Process above-mentioned dry suspending agent wet feed with spray drying method, operating parameter is：Hothouse inlet temperature：95℃；Hothouse outlet temperature
Degree：80℃.
The above-mentioned dry suspending agent preparing is positioned over 0d, 7d, 14d, 21d in 54 DEG C of baking ovens and measures bacterium amount alive, institute respectively
Obtain result of the test and be shown in Table 1.
Table 1 Paecilomyces lilacinus dry suspending agent Accelerated stability test
According to table 1 data display, the adjuvant that the present invention provides, auxiliary agent compound use can delay Paecilomyces lilacinus to do suspension
Bacterium number alive declines, and improves medicine stability.
Embodiment 12
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is Hylocereus undatuss, and management is good, and root-knot nematode occurs
Medium weighting.Experimental field is divided into four regions by this test, and region 1 is matched group, only along ridge spray clear water；Region 2 is suitable
Ridge sprays 1000 times of diluents of Paecilomyces lilacinus of preparation in embodiment 8；Region 3 sprays Paecilomyces lilacinus wettable powder along ridge
1000 times of diluents, the concentration of medicine activity component is identical with region 2；Region 4 sprays Paecilomyces lilacinus aqueous suspension agent along ridge
1000 times of diluents, the concentration of medicine activity component is identical with region 2 and 3；The dosage in four regions is identical, 1 day after dispenser,
3 days, 5 days, each investigation in 7 days once borer population alive, calculate Revision insect recluced rate.As shown in table 2, the data by table 2 is permissible for concrete outcome
Draw, the Revision insect recluced rate highest in region 2, show the insecticidal effect of Paecilomyces lilacinus wettable powder of present invention preparation
Good, its drug effect is above wettable powder and the aqueous suspension agent of same concentration.
Parasite killing (root-knot nematode) effect of table 2 Paecilomyces lilacinus dry suspending agent
As seen from the above embodiment, the Paecilomyces lilacinus dry suspending agent stability of present invention preparation is high, and viable count is high, to line
The control rate of worm may be up to more than 84.7%, and its drug effect is above wettable powder and the aqueous suspension agent of same concentration.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Paecilomyces lilacinus dry suspending agent, including effective ingredient, auxiliary agent and adjuvant；
Described effective ingredient is Paecilomyces lilacinus, and the Paecilomyces lilacinus spore content in described dry suspending agent is 1.0～500.0 hundred million
Individual/gram.
2. dry suspending agent according to claim 1 it is characterised in that described adjuvant be selected from sucrose, glucose, analysis for soybean powder,
One or more of rice meal, wheat flour, corn starch, potato starch, Testa Tritici, vitamin C, citric acid and salicylic acid.
3. dry suspending agent according to claim 1 it is characterised in that described auxiliary agent be selected from polymerization of carboxylic acid salt, naphthalene sulfonate,
Fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, phenethyl phenol polyethenoxy ether, iso-octyl succinate, sulfate, phosphorus
One or more of sour monohydric salt and nitrate.
4. the dry suspending agent according to claims 1 to 3 any one is it is characterised in that the quality of described adjuvant and auxiliary agent
Than for (30～65)：(5～15).
5. the dry suspending agent according to claims 1 to 3 any one is it is characterised in that total matter of described auxiliary agent and adjuvant
Amount is (50～99) with the mass ratio of effective ingredient:(1～50).
6. dry suspending agent according to claim 5 is it is characterised in that the gross mass of described auxiliary agent and adjuvant and effective ingredient
Mass ratio be (58～90):(10～42).
7. the preparation method of dry suspending agent described in claim 1～6 any one, comprises the following steps：
A, Paecilomyces lilacinus are carried out solid fermentation, the solid medium obtaining is mixed with water, fermented after described fermentation
Slurry；
B, auxiliary agent and adjuvant are mixed and pulverize after, mix with the fermentation slurry obtaining in described step a, obtain the dry mixing that suspends
Slurry；
C, the dry suspension mixed solution slurry obtaining described b step are spray-dried, and obtain Paecilomyces lilacinus dry suspending agent.
8. according to claim 7 preparation method it is characterised in that：After pulverizing in described step b, the material particular diameter that obtains is
D95≤ 10 microns.
9. according to claim 8 preparation method it is characterised in that：The inlet temperature being spray-dried in described step c：90～
110 DEG C, the outlet temperature of described spray drying：80～90 DEG C.
10. application in preventing and treating crop nematodiasis for the dry suspending agent described in claim 1～6 any one.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN110745865A (en) * | 2019-11-12 | 2020-02-04 | 四川天地同光科技有限责任公司 | Water-based dispersion suspension method for fine particles |
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| CN102422844A (en) * | 2011-09-27 | 2012-04-25 | 德强生物股份有限公司 | Nematode control composition containing paecilomyces lilacinus |
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| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN110745865A (en) * | 2019-11-12 | 2020-02-04 | 四川天地同光科技有限责任公司 | Water-based dispersion suspension method for fine particles |
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Application publication date: 20170208 |