CN103749545A - Isaria fumosorosea and bacillus thuringiensis wettable powder - Google Patents
Isaria fumosorosea and bacillus thuringiensis wettable powder Download PDFInfo
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Abstract
The invention discloses isaria fumosorosea and bacillus thuringiensis wettable powder. The wettable powder comprises the following ingredients in percentage by mass: 8%-20% of isaria fumosorosea and bacillus thuringiensis, 1-8% of wetting agent, 1-12% of dispersing agent, 0.1-0.5% of ultraviolet protective agent, and the balance of carriers. The weight part ratio of the isaria fumosorosea to bacillus thuringiensis is (10:0.4) to (10:1). The isaria fumosorosea is isaria fumosorosea strain SCAU-IFCF02 which is preserved in CCTCC (China Center for Type Culture Collection) at November 1rst, 2013, with the preservation number of CCTCC NO:M2013526. The bacillus thuringiensis is subsp. Kurstaki. The wettable powder is fast and efficient, has no pesticide residues, postpones the occurrence of pest resistance to insecticide, is environment-friendly, and especially has a good prevention effect on pests on brassicaceous vegetables of plutella xylostella and the like.
Description
Technical field
The present invention relates to mixed biologic pesticide field.More specifically, relate to a kind of rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder.
Background technology
The resistance problem of pest is one of global hot issue, is also the emphasis problem that agricultural science and technology worker pays close attention to always.At present to agricultural insect management mainly take chemical control as main, the increase of agricultural chemicals usage amount and non-science are used, and cause pest resistance to insecticide day by day serious, the pest species that produces resistance is more and more, and natural enemy is produced to adverse effect.Meanwhile, high-intensity use agricultural chemicals, makes that Practice for Pesticide Residue in Agricultural Products exceeds standard, the increase of peasant's drug cost, contaminated environment and the mankind and domestic animal are produced to harmful effect etc., is unfavorable for agriculture sustainable development.Therefore, research and development high-efficiency environment friendly, agricultural chemicals pollution-free, noresidue have great importance to agricultural sustainable development.
Rose dark brown Isaria (
isaria fumosorosea) be a kind of important insect pathogenic fungus, be distributed widely in the torrid zone, subtropics and Temperate Region in China; Host range is wide, can comprise various vegetables, fruit tree and the Pests of Tea-Plants such as Homoptera, diptera, Lepidoptera, Semiptera, coleoptera and Hymenoptera by parasitism.Rose dark brown Isaria is as a kind of living body biological agricultural chemicals, have be different from the brand-new mechanism of action of existing chemical insecticide, to pest efficient, environmentally friendly, the advantage such as be difficult for developing immunity to drugs, therefore become the focus of domestic and international biological control research.And, with fungi microbe pesticide control plant pest, be one of important means in the sustainable production of non-polluted farm product, have broad application prospects.But fungi preparation is the prerequisite of its microbial pesticide application, since the sixties in 20th century, there are 10 kinds of rose dark brown Isaria formulation products to be found out and to obtain registration abroad, in recent years come out commodity preparation had conidium preparation Biocon(Holland), AgoBiocontrol Bassiana 50(Colombia), Pae-sin(Mexico), Bemisin(Venezuela), blastopore preparation PFR-97 and blastopore and mycelial mix preparation PreFeRal.Although these existing rose dark brown Isaria preparations have been obtained good control efficiency to insect,, the same with other biocontrol fungis, slow this shortcoming of these preparation as insecticiding speed has seriously restricted its application in field.
Bacillus thuringiensis,Bt (
bacillus thuringiensisbe called for short Bt) be to use in the world the widest, the maximum biological insecticides of output, its main active is one or several insecticidal crystal proteins (insecticidal crystal proteins, ICPs), claim again delta-endotoxin, the arthropodss such as the insects such as Lepidoptera, coleoptera, Hymenoptera, Homoptera and animals and plants nematode, acarian are had to good specificity cytotoxicity, and to non-target organism without adverse effect.Biotrol BTV is efficient, single-minded, to person poultry safety, the advantage such as environmentally friendly, make Su Yun gold bar commodity preparation bacterium become consumption maximum in the world, and the microorganism insecticide that the scope of application is wide, the most respond well, enjoys people to pay close attention to.But commodity thuricade-1 also exists quick-acting poor in production control, to insensitive, the shortcoming such as the field lasting period is short, easily produce resistance and recombinant strain genetic character is unstable of insect at advanced age, become and affected the restraining factors that bacillus thuringiensis,Bt is further promoted the use of.
As everyone knows, diamond-back moth (
plutella xylostellal.) be one of the important pests of worldwide brassicaceous vegetable, its host is nearly more than 40 kinds, and main harm brassicaceous vegetable year occurs from generation to generation manyly, and reproduction rate is high, and generation overlap phenomenon is serious.At present to the control of diamond-back moth still take chemical control as main, the continuous use of a large amount of chemical pesticides, making diamond-back moth be in all the time stronger medicament selects to depress, add that again its reproduction speed is fast, cause its resistance level to medicament more and more higher, now become pesticide resistance the most serious and one of the insect of the most difficult control, have a strong impact on output and the quality of vegetables.At present, diamond-back moth has produced resistance in various degree to more than 50 kinds of insecticides, and comprehensive regulation situation is very severe.
Summary of the invention
The technical problem to be solved in the present invention is the technical deficiency that overcomes the Cruciferous Vegetable Pests controls such as existing diamond-back moth, and one novel built insecticide is fast and efficiently provided.
The object of the invention is to provide a kind of rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder.
Another object of the present invention is to provide the application of described wetting powder.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
The invention provides a kind of rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder, described wetting powder is grouped into by the one-tenth of following quality percentage composition: 8～20% rose dark brown Isaria and bacillus thuringiensis,Bts, 1～8% wetting agent, 1～12% dispersant, 0.1～0.5% uv-protector, surplus is carrier;
Described rose dark brown Isaria and the weight part ratio of bacillus thuringiensis,Bt are 10:0.4～10:1.More preferably, described rose dark brown Isaria and the weight part ratio of bacillus thuringiensis,Bt are 10:0.6～10:0.9.
Preferably, described wetting powder is grouped into by the one-tenth of following quality percentage composition: 10～18% rose dark brown Isaria and bacillus thuringiensis,Bts, and 2～6% wetting agents, 2～10% dispersants, 0.2～0.4% uv-protector, surplus is carrier; Described rose dark brown Isaria and the weight part ratio of bacillus thuringiensis,Bt are 10:0.6～10:0.9.
Described rose dark brown Isaria preferably adopt rose dark brown Isaria (
isaria fumosorosea(Wize) Brown & Smith) bacterial strain SCAU-IFCF02, this bacterial strain is preserved in Chinese Typical Representative culture collection center on November 1st, 2013, deposit number is CCTCC NO:M 2013526, and preservation address is Wuhan, China city Wuhan University.
Described rose dark brown Isaria is that spore content is the rose dark brown Isaria conidial powder of 12,400,000,000/g.
Described bacillus thuringiensis,Bt is bacillus thuringiensis,Bt Kurstaki, and quality is 50000IU/mg, commercial.
Described wetting agent is octyl phenol APEO-10(OP-10), wetting agent Morwet EFW(alkylnaphthalene sulfonate and anion wetting agent mixture, Akzo Nobel N.V. produces), one or more the mixture in fatty alcohol-polyoxyethylene ether (AEO-9).
Described dispersant is one or more the mixture in fatty alcohol-polyoxyethylene ether, methylene two bitter edible plant sodium sulfonates, alkylnaphthalene sulfonate condensation polymer (Morwet D-425, Akzo Nobel N.V. produces).
Described uv-protector is one or more the mixture in fluorescein sodium, xanthans, ascorbic acid.
Described carrier is one or both the mixture in talcum powder, diatomite.
Preferably, described wetting agent is one or both the mixture in octyl phenol APEO-10 or Morwet EFW.
Preferably described dispersant is one or both the mixture in methylene two bitter edible plant sodium sulfonates or Morwet D-425.
Preferably described uv-protector is ascorbic acid.
Preferably described carrier is talcum powder.
The present invention also provides described rose dark brown Isaria and the application of bacillus thuringiensis,Bt wetting powder aspect the preparation of preparation control Homoptera, diptera, Lepidoptera, Semiptera, coleoptera or Hymenoptera insect.
Preferably, described insect is Cruciferous Vegetable Pests.
More preferably, described insect is diamond-back moth, red fire ant or Bemisia tabaci.
Preferably, described Cruciferous Vegetable Pests is diamond-back moth, cabbage caterpillar, aphid, Bemisia tabaci.
Preferably, the preparation method of rose dark brown Isaria conidial powder of the present invention is as follows:
S1. one-level solid culture, coats SDAY solid culture medium (SDAY medium is optimum medium) by rose dark brown Isaria bacterial strain SCAU-IFCF02, cultivates in 24 ℃, and best incubation time is 15 days;
S2. secondary liquid oscilaltion is cultivated, and with the spore on above-mentioned solid culture medium under the aseptic washing that contains 0.03% Tween-80, then with sterile gauze filtration, makes 1 × 10
7the suspension of individual spore/mL, the above-mentioned suspension of 100uL is inoculated in the Sa Shi culture fluid of 200mL (be called for short SDB, be the suitableeest inoculation liquid), 24 ℃, 170r/min shaken cultivation 72 hours;
S3. three grades are produced spore cultivation, low value old rice (common rice, be purchased from the Agricultural University Of South China market of farm produce) the quality distilled water such as add, cooling standby after 121 ℃ of moist heat sterilization 30min, the bacterium liquid that contains a large amount of mycelia that S2 is obtained accesses on the rice of steam sterilizing, bacterium liquid is laid in the pallet that is lined with gauze after fully mixing with low value old rice, and covers 24 ℃ of dark culturing 6 days with preservative film.When media surface covers with mycelia, throw off preservative film and continue to cultivate 4 days, make the large volume production spore of mycelia, results conidial powder, vacuum cooling drying 24 hours, dried Conidial storage is in sterile chamber, and 4 ℃ save backup.The conidial powder spore content obtaining is 12,400,000,000/g, and the spore rate of living is 97.8%, and water content is 2.56%, and outward appearance is light rose dark brown powder.
Wherein, described in S1, SDAY solid culture medium compound method is as follows:
Glucose 40g/L, peptone 10g/L, yeast extract 20g/L, agar 15～20g/L, H
20 1L, stirring and evenly mixing, then packing, sterilizing, standby.
Described in S2, Sa Shi culture fluid compound method is as follows:
Glucose 40g/L, peptone 10g/L, yeast extract 20g/L, H
201L, stirring and evenly mixing, then packing, sterilizing, standby.
Inventor herein separates the rose dark brown Isaria bacterial strain SCAU-IFCF02 obtaining, and diamond-back moth is had to low-down lethal concentration and the fastest lethal speed, has overcome slow these restraining factors of common fungi microbe agricultural insecticide speed.Therefore, the present invention infects body conidium as active component take the common of rose dark brown Isaria bacterial strain SCAU-IFCF02, be prepared into rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder, can lay the foundation for the industrialization of rose dark brown Isaria bacterial strain SCAU-IFCF02, for the field application of this bacterial strain provides theoretical direction, have a good application prospect.
The collocation of rose dark brown Isaria and bacillus thuringiensis,Bt is used, and is used for preventing and treating the Cruciferous Vegetable Pests such as diamond-back moth, there is no at present report and application.The collocation of different pesticide species and different insect killing substances is used, and can effectively improve control efficiency, reduces dosage, reduces costs, and delays the generation of pest resistance to insecticide, the service life of prolongation medicament, is the important means of integrated pest management.The inventor is on the basis of a large amount of indoor bioassay and field medicine effect test, find that bacillus thuringiensis,Bt and rose dark brown Isaria SCAU-IFCF02 bacterial strain have good compatibility, and rose dark brown Isaria provided by the invention and bacillus thuringiensis,Bt wetting powder are as a kind of biological insecticides, desinsection speed to target pest and successful are better than alone rose dark brown Isaria and bacillus thuringiensis,Bt, there is different desinsection modes and desinsection object, the two is checked and obtains novel compounded pesticides, expanded insecticidal spectrum, improve insecticide efficiency, slowing down pest resistance to insecticide produces, reduce the use of chemical pesticide.Prepared insecticide has the brand-new mechanism of action that is different from existing insecticide, and insecticidal effect is remarkable, especially remarkable for the toxic action of Cruciferous Vegetable Pests diamond-back moth, has environmental friendliness, to person poultry safety's feature simultaneously.Therefore, the present invention infects body conidium and bacillus thuringiensis,Bt as active component take rose dark brown Isaria bacterial strain SCAU-IFCF02 common, be prepared into rose dark brown cluster bacillus thuringiensis,Bt spore wetting powder, obtained extraordinary pest control effect, for the industrialization of rose dark brown Isaria preparation lays the foundation, for the field application of said preparation provides theoretical direction.
The present invention has following beneficial effect:
In rose dark brown Isaria provided by the invention and bacillus thuringiensis,Bt wetting powder, bacillus thuringiensis,Bt and rose dark brown Isaria spore have good compatibility, synergistic effect is obvious, desinsection speed to target pest and successful are better than alone rose dark brown Isaria and bacillus thuringiensis,Bt, reach that a medicine is controlled more, the effect of quick-acting guarantor's liquid.Desinsection collection of illustrative plates expands, and except control diamond-back moth, can also prevent and treat other Cruciferous Vegetable Pests such as cabbage caterpillar, aphid, Bemisia tabaci, and this wetting powder can superinfection, successful in the non-environmental pollution control of insect and lasting integrated control.
In addition, wettable powder agent producing process of the present invention is simple, can significantly reduce the usage amount of agricultural chemicals, delay pest resistance to insecticide, reduce residue of pesticide, to person poultry safety, environmentally friendly, to agricultural production and income, improve the ecological environment, develop environmental protection agricultural, to realizing the strategy of sustainable development, have great importance.
Embodiment
Below in conjunction with specific embodiment, further illustrate the present invention, but embodiment does not limit in any form to the present invention.Unless stated otherwise, reagent, the method and apparatus that the present invention adopts is the conventional reagent of the art, method and apparatus.
Unless stated otherwise, following examples agents useful for same and raw material are conventional method preparation or commercial.
Embodiment of the present invention rose dark brown used Isaria is that spore content is the rose dark brown Isaria conidial powder of 12,400,000,000/g.
The present invention is as follows to the assay method of spore germination rate: spore suspension is inoculated into fungi germination fluid (glucose 20g/L, peptone 5g/L, H
20 1L) in, 24 ℃, 170 r/min, microscopy statistics spore rate alive after 18h.Wetting time is measured according to GB/T5451-2001 standard method.Suspensibility is pressed GB/T 14825-2006 standard method and is measured.PH value is pressed GB/T1600-93 standard method and is measured.Fineness is measured by laser particle distribution instrument.Data statistic analysis: all experimental datas are analyzed by DPS data handling system, computation of mean values and standard error, the average significance of difference is carried out multiple analysis comparison by Duncan duncan's new multiple range method.
The used sterile water that contains 0.03% Tween-80 in embodiment, described 0.03% refers to volume ratio.
the preparation of embodiment 1 rose dark brown Isaria conidial powder
The present embodiment adopts solid-liquid double-phase fermented and cultured, prepares rose dark brown Isaria conidial powder, and preparation process is as follows:
S1. one-level solid culture, gets the original bacterium liquid of 50uL rose dark brown Isaria bacterial strain SCAU-IFCF02 and coats (SDAY medium is optimum medium) on SDAY solid culture medium, cultivates in 24 ℃, and best incubation time is 15 days;
S2. secondary liquid oscilaltion is cultivated, and with the spore on SDAY solid culture medium under the aseptic washing that contains 0.03% Tween-80, then with double gauze filtration, makes 1 × 10
7the suspension of individual spore/mL, the above-mentioned suspension of 100uL is inoculated in the Sa Shi culture fluid of 200mL (be called for short SDB, be the suitableeest inoculation liquid), 24 ℃, 170r/min shaken cultivation 72 hours;
S3. three grades are produced spore cultivation, low value old rice (common rice, be purchased from the Agricultural University Of South China market of farm produce) the quality distilled water such as add, cooling standby after 121 ℃ of moist heat sterilization 30min, on the rice of the bacterium liquid that the contains a large amount of mycelia access sterilizing that S2 is obtained, bacterium liquid is laid in the pallet that is lined with gauze after fully mixing with low value old rice, and covers 24 ℃ of dark culturing 6 days with preservative film.When media surface covers with mycelia, throw off preservative film and continue to cultivate 4 days, make the large volume production spore of mycelia, results conidial powder, vacuum cooling drying 24 hours, dried Conidial storage is in sterile chamber, and 4 ℃ save backup.
The conidial powder spore content obtaining is 12,400,000,000/g, and the spore rate of living is 97.8%, and water content is 2.56%, and outward appearance is light rose dark brown powder.
Described in step S1, SDAY solid culture medium compound method is as follows:
Glucose 40g/L, peptone 10g/L, yeast extract 20g/L, agar 15～20g/L, H
20 1L, stirring and evenly mixing, then packing, sterilizing, standby.
Described in step S2, Sa Shi culture fluid compound method is as follows:
Glucose 40g/L, peptone 10g/L, yeast extract 20g/L, H
2o 1L, stirring and evenly mixing, then packing, sterilizing, standby.
the preparation of embodiment 2 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, preparation method:
S1. accurately take rose dark brown Isaria conidial powder and Bacillus thuringiensis (commercial) prepared by embodiment 1;
S2. various auxiliary agents are joined in low-temperature airflow cracker, control the temperature of air-flow crushing below 10 ℃, standby after pulverizing; Preferably according to 325 order finess disintegratings,
S3. proportionally S2 is pulverized to the various agent mixture that obtain and join in carrier, stir, then add the load weighted rose dark brown of S1 Isaria conidial powder and Bacillus thuringiensis, after mixing, obtain the wetting powder of mixture.
2, the prepared wetting powder of the present embodiment comprises the constituent of following percentage by weight: rose dark brown Isaria 9.2%, bacillus thuringiensis,Bt 0.8%, OP-10 3%, Morwet EFW 2%, methylene two bitter edible plant sodium sulfonates 3%, Morwet D-425 5%, ascorbic acid 0.3%, talcum powder 76.7%.
3, the performance indications of the prepared wetting powder of the present embodiment are measured
Testing result shows, product performance index is good, rose dark brown Isaria spore germination rate >=85; Bacillus thuringiensis Spore germination rate is 84.17%, wetability <1min; Quality suspensibility is 72.3; The suspensibility of spore is 85.5, and moisture is 2.56; Fineness (crossing 325 mesh standard sieves) >=98; PH value is 6.2.The performance indications of the wetting powder of preparing meet the requirement of pesticidal preparations commodity.
the preparation of embodiment 3 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, preparation method is with embodiment 2.
2, the prepared wetting powder of the present embodiment comprises the constituent of following percentage by weight: rose dark brown Isaria 11.3%, bacillus thuringiensis,Bt 0.7%, AEO-9 4%, Morwet EFW 2%, fatty alcohol-polyoxyethylene ether 2%, Morwet D-425 7%, fluorescein sodium 0.3%, diatomite 45.9%, talcum powder 26.8%.
3, the performance indications of the prepared wetting powder of the present embodiment are measured
Testing result shows, product performance index is good, rose dark brown Isaria spore germination rate >=85; Bt gemma germination rate is 82.17%, wetability <1min; Quality suspensibility is 71.9; The suspensibility of spore is 86.7, and moisture is 2.96; Fineness (crossing 325 mesh standard sieves) >=98; PH value is 6.5.The performance indications of the wetting powder of preparing meet the requirement of pesticidal preparations commodity.
the preparation of embodiment 4 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, preparation method is with embodiment 2.
2, the prepared wetting powder of the present embodiment comprises the constituent of following percentage by weight: rose dark brown Isaria 13.8%, bacillus thuringiensis,Bt 1.2%, OP-10 2%, Morwet EFW 2%, fatty alcohol-polyoxyethylene ether 2%, methylene two bitter edible plant sodium sulfonates 3%, Morwet D-425 6%, xanthans 0.5%, talcum powder 69.5%.
3, the performance indications of the prepared wetting powder of the present embodiment are measured
Testing result shows, product performance index is good, rose dark brown Isaria spore germination rate >=85; Bt gemma germination rate is 86.17%, wetability <1min; Quality suspensibility is 74.3; The suspensibility of spore is 87.5, and moisture is 3.06; Fineness (crossing 325 mesh standard sieves) >=98; PH value is 6.4.The performance indications of the wetting powder of preparing meet the requirement of pesticidal preparations commodity.
the preparation of embodiment 5 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, preparation method is with embodiment 2.
2, the prepared wetting powder of the present embodiment comprises the constituent of following percentage by weight: rose dark brown Isaria 17%, bacillus thuringiensis,Bt 1%, OP-10 5%, Morwet EFW 3%, fatty alcohol-polyoxyethylene ether 2%, methylene two bitter edible plant sodium sulfonates 4%, Morwet D-425 5%, ascorbic acid 0.5%, talcum powder 30%, diatomite 32.5%.
3, the performance indications of the prepared wetting powder of the present embodiment are measured
Testing result shows, product performance index is good, rose dark brown Isaria spore germination rate >=85; Bt gemma germination rate is 84.17%, wetability <1min; Quality suspensibility is 72.3; The suspensibility of spore is 85.5, and moisture is 2.56; Fineness (crossing 325 mesh standard sieves) >=98; PH value is 6.2.The performance indications of the wetting powder of preparing meet the requirement of pesticidal preparations commodity.
the preparation of embodiment 6 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, preparation method is with embodiment 2.
2, the prepared wetting powder of the present embodiment comprises the constituent of following percentage by weight: rose dark brown Isaria 18%, bacillus thuringiensis,Bt 2%, OP-10 4%, Morwet EFW 4%, fatty alcohol-polyoxyethylene ether 2%, methylene two bitter edible plant sodium sulfonates 5%, Morwet D-425 5%, ascorbic acid 0.4%, talcum powder 59.6%.
3, the performance indications of the prepared wetting powder of the present embodiment are measured
Testing result shows, product performance index is good, rose dark brown Isaria spore germination rate >=85; Bt gemma germination rate is 85.37%, wetability <1min; Quality suspensibility is 74.3; The suspensibility of spore is 85.5, and moisture is 2.78; Fineness (crossing 325 mesh standard sieves) >=98; PH value is 6.5.The performance indications of the wetting powder of preparing meet the requirement of pesticidal preparations commodity.
the evaluating drug effect of embodiment 7 rose dark brown Isarias and bacillus thuringiensis,Bt wetting powder
1, for examination material
(1) for examination insect: diamond-back moth
plutella xylostella(L.) 2 instar larvaes.
(2) rose dark brown Isaria prepared by reagent agent: embodiment 2 and bacillus thuringiensis,Bt wetting powder.
Inventor is through Toxicity Determination experiment, result shows, rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder that above-described embodiment 2～6 is prepared all have extraordinary control efficiency to diamond-back moth, when wettable powder agent concentration is while diluting 500, from the 1st day diamond-back moth connecing bacterium, just start death, the corrected mortality of the 4th day diamond-back moth is all between 90%～100%.
Because data are too many, the present invention only provides the data of wetting powder control diamond-back moth prepared by embodiment 2, is enough to illustrate that rose dark brown Isaria provided by the invention and bacillus thuringiensis,Bt wetting powder have good control efficiency to insect.
(3) contrast medicament: the rose dark brown Isaria wetting powder single dose preparing with embodiment 2 preparation methods and bacillus thuringiensis,Bt wetting powder single dose.
(4) for studying thing: cabbage mustard (
b. alboglabrabailey).
2, rose dark brown Isaria and the Toxicity Determination of bacillus thuringiensis,Bt wetting powder to diamond-back moth
(1) experimental technique
500 times, 1000 times, 2000 times and 5000 times four concentration of rose dark brown Isaria and bacillus thuringiensis,Bt wettable powder dilution agent.
Adopt dip method to carry out biologicall test: to choose Individual Size, diamond-back moth 2 instar larvaes that vigor is consistent, with banister bruss, will supply to try larva choose on clean cabbage mustard blade, then blade and larva are put into the wetting powder of different extension rates, after dipping 10s, choose, larva is placed on the filter paper of sterilizing and blots after excessive moisture, move on blade, put into the culture dish (diameter is 9cm) that bottom is lined with moistening filter paper, culture dish seals moisturizing with preservative film, on film, prick hole ventilation, be placed in artificial intelligence case, 24 ℃, relative moisture (RH) 90%, periodicity of illumination is illumination: dark=14:10.20 larvas of every processing, repeat 5 times, 100 larvas totally.Take wetting powder basic components (not comprising the wetting powder of rose dark brown Isaria conidial powder and bacillus thuringiensis,Bt) as blank, take rose dark brown Isaria wetting powder single dose and bacillus thuringiensis,Bt wetting powder single dose, dilute 500 times as medicament contrast.After 24 hours, start to observe, depending on ware intra vane, by the situation of taking food, changed fresh cabbage leaves, record susceptible symptom and dead borer population every day, Continuous Observation 6 days, calculates lethality and corrected mortality.
Lethality (%)=dead borer population/each concentration is processed examination worm sum × 100
Corrected mortality (%)=(processing lethality-contrast lethality)/(1-contrasts lethality) × 100
(2) Toxicity Determination result
Rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder are as shown in table 1 to the Toxicity Determination result of diamond-back moth.
Table 1 rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder are to diamond-back moth 2 instar larvae toxicity test results
As shown in table 1, after medication 24h there is obvious death in diamond-back moth, after 24h, 48h, 72h, 96h and 120h, rose dark brown Isaria of the present invention and bacillus thuringiensis,Bt wetting powder are all significantly higher than single dose and blank to diamond-back moth 2 instar larvae lethality, and corrected mortality is up to 100%, shown that rose dark brown Isaria of the present invention and bacillus thuringiensis,Bt wetting powder have extraordinary control efficiency to diamond-back moth.
3, the field trial of rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder control diamond-back moth
(1) test method
Experiment arranges 15Ge community altogether, 16 square metres of each community areas, and random district group is arranged.500 times, 1000 times, 2000 times three concentration of rose dark brown Isaria and bacillus thuringiensis,Bt wettable powder dilution agent, take bacillus thuringiensis,Bt wetting powder and rose dark brown Isaria wetting powder single dose, dilute 500 times as medicament contrast, with clear water, be treated to blank, each processing repeats 3 times.Divide community even spraying with commercially available knapsack hand sprayer.Per hectare amount of liquid medicine 900kg, every community water 1.45kg.
Test adopts 5 methods to investigate, the sampling of 5 of every communities, every 2 strain fixed point investigation, investigation insect population radix before medicine, after medicine, 1,3,7 day residual borer population of investigation diamondback moth larvae, calculates insect population decline rate and relative control effect, and the analysis of all data all adopts DPS software to complete.
Borer population × 100 alive before insect population decline rate (%)=(borer population of living after the borer population-medicine of living before medicine)/medicine
Control efficiency (%)=(PT-CK)/(100-CK) × 100
In formula: CK is blank district insect population decline rate, PT is chemicals treatment district insect population decline rate.
(2) result of the test
Rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder are as shown in table 2 to the field efficacy of diamond-back moth.
Table 2 rose dark brown Isaria bacillus thuringiensis,Bt wetting powder control diamond-back moth field control effectiveness test result
As shown in table 2, rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder have significant control efficiency to diamond-back moth, and using dosage is for dilution is 500 times time, and after medicine, the preventive effect of the 1st day, 3 days and 7 days is respectively 52.47%, 69.72%, 89.93%; Its using dosage is for dilution is 1000 times time, and after medicine, the preventive effect of the 1st day, 3 days and 7 days is respectively 52.86%, 64.94%, 85.40%, all good than contrast effect of chemical control.As the environmentally safe microbial pesticide of one, rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder have great exploitation prospect, and the Cruciferous Vegetable Pests such as diamond-back moth are had to the persistence that extraordinary preventive effect is become reconciled.
In addition, according to duration of test field observation, each treatment region is normal for examination plant growth, does not find any harmful effect, and other non-target organisms are also had no adverse effects.And find by data survey, each treatment region has cabbage caterpillar, Bemisia tabaci to mix harm, after dispenser, the quantity of cabbage caterpillar and Bemisia tabaci also significantly declines, illustrate that rose dark brown Isaria of the present invention and bacillus thuringiensis,Bt wetting powder have good control efficiency to other insects such as cabbage caterpillar and Bemisia tabaci simultaneously, desinsection collection of illustrative plates is larger, can control by a medicine, and this wetting powder can superinfection more, successful in the non-environmental pollution control of insect and lasting integrated control.
Claims (10)
1. a rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described wetting powder is grouped into by the one-tenth of following quality percentage composition: 8～20% rose dark brown Isaria and bacillus thuringiensis,Bts, 1～8% wetting agent, 1～12% dispersant, 0.1～0.5% uv-protector, surplus is carrier;
Described rose dark brown Isaria and the weight ratio of bacillus thuringiensis,Bt are 10:0.4～10:1.
2. rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder according to claim 1, it is characterized in that, described wetting powder is grouped into by the one-tenth of following quality percentage composition: 10～18% rose dark brown Isaria and bacillus thuringiensis,Bts, 2～6% wetting agents, 2～10% dispersants, 0.2～0.4% uv-protector, surplus is carrier;
Described rose dark brown Isaria and the weight ratio of bacillus thuringiensis,Bt are 10:0.6～10:0.9.
3. according to rose dark brown Isaria described in claim 1 or 2 and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described rose dark brown Isaria is rose dark brown Isaria bacterial strain SCAU-IFCF02, this bacterial strain is preserved in Chinese Typical Representative culture collection center on November 1st, 2013, and deposit number is CCTCC NO:M 2013526.
4. according to rose dark brown Isaria described in claim 1 or 2 and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described rose dark brown Isaria is that spore content is the rose dark brown Isaria conidial powder of 12,400,000,000/g.
5. according to rose dark brown Isaria described in claim 1 or 2 and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described bacillus thuringiensis,Bt is bacillus thuringiensis,Bt Kurstaki.
6. according to rose dark brown Isaria described in claim 1 or 2 and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described wetting agent is one or more the mixture in octyl phenol APEO-10, Morwet EFW or fatty alcohol-polyoxyethylene ether;
Described dispersant is one or more the mixture in fatty alcohol-polyoxyethylene ether, methylene two bitter edible plant sodium sulfonates or Morwet D-425;
Described uv-protector is one or more the mixture in fluorescein sodium, xanthans or ascorbic acid;
Described carrier is one or both the mixture in talcum powder or diatomite.
7. according to rose dark brown Isaria described in claim 1 or 2 and bacillus thuringiensis,Bt wetting powder, it is characterized in that, described wetting agent is one or both the mixture in octyl phenol APEO-10 or Morwet EFW;
Described dispersant is one or both the mixture in methylene two bitter edible plant sodium sulfonates or Morwet D-425;
Described uv-protector is ascorbic acid;
Described carrier is talcum powder.
8. the arbitrary described rose dark brown Isaria of claim 1～7 and the bacillus thuringiensis,Bt wetting powder application aspect the preparation of preparation control Homoptera, diptera, Lepidoptera, Semiptera, coleoptera or Hymenoptera insect.
9. application according to claim 8, is characterized in that, described insect is Cruciferous Vegetable Pests.
10. application according to claim 8, is characterized in that, described insect is diamond-back moth, red fire ant or Bemisia tabaci.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026123A (en) * | 2014-06-30 | 2014-09-10 | 西南林业大学 | Wettable powder for preventing and controlling tomicus yunnanensis compound spores and preparation method of wettable powder |
| CN104642392A (en) * | 2015-01-13 | 2015-05-27 | 华南农业大学 | Isaria fumosorosea conidia wettable powder and application thereof |
| CN105602867A (en) * | 2016-01-18 | 2016-05-25 | 黑龙江省科学院微生物研究所 | Slow-growing soybean rhizobium protective agent |
| CN106591146A (en) * | 2016-12-02 | 2017-04-26 | 华中农业大学 | Fermentation culture method for isaria fumosorosea |
| CN108770870A (en) * | 2018-06-13 | 2018-11-09 | 马瑞燕 | A kind of fumosorosea water dispersible granules and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1306008A1 (en) * | 2001-10-24 | 2003-05-02 | Natural Bio Technology Co. | Composition for treatment against mosquito larvae and process for its preparation |
| CN102246750A (en) * | 2011-05-20 | 2011-11-23 | 华南农业大学 | Isaria fumosorosea oil suspending agent and preparation method as well as application thereof |
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN103160450A (en) * | 2011-12-15 | 2013-06-19 | 河北农业大学 | Broad-spectrum efficient novel bacillus thuringiensis strain, microbial inoculum and application thereof |
| CN103444786A (en) * | 2013-08-22 | 2013-12-18 | 广东省农业科学院植物保护研究所 | Bacillus thuringiensis and isaria fumosorosea compounded suspending agent and preparation method thereof |
-
2013
- 2013-12-24 CN CN201310721075.4A patent/CN103749545B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1306008A1 (en) * | 2001-10-24 | 2003-05-02 | Natural Bio Technology Co. | Composition for treatment against mosquito larvae and process for its preparation |
| CN102246750A (en) * | 2011-05-20 | 2011-11-23 | 华南农业大学 | Isaria fumosorosea oil suspending agent and preparation method as well as application thereof |
| CN103160450A (en) * | 2011-12-15 | 2013-06-19 | 河北农业大学 | Broad-spectrum efficient novel bacillus thuringiensis strain, microbial inoculum and application thereof |
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN103444786A (en) * | 2013-08-22 | 2013-12-18 | 广东省农业科学院植物保护研究所 | Bacillus thuringiensis and isaria fumosorosea compounded suspending agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王磊 等: "11种杀虫剂对草皮中红火蚁的检疫除害效果", 《植物检疫》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026123A (en) * | 2014-06-30 | 2014-09-10 | 西南林业大学 | Wettable powder for preventing and controlling tomicus yunnanensis compound spores and preparation method of wettable powder |
| CN104642392A (en) * | 2015-01-13 | 2015-05-27 | 华南农业大学 | Isaria fumosorosea conidia wettable powder and application thereof |
| CN104642392B (en) * | 2015-01-13 | 2018-05-01 | 华南农业大学 | A kind of fumosorosea bacterium conidium wettable powder and its application |
| CN105602867A (en) * | 2016-01-18 | 2016-05-25 | 黑龙江省科学院微生物研究所 | Slow-growing soybean rhizobium protective agent |
| CN105602867B (en) * | 2016-01-18 | 2018-11-02 | 黑龙江省科学院微生物研究所 | A kind of Slow-growing Soybean rhizobia protective agent |
| CN106591146A (en) * | 2016-12-02 | 2017-04-26 | 华中农业大学 | Fermentation culture method for isaria fumosorosea |
| CN106591146B (en) * | 2016-12-02 | 2019-06-18 | 华中农业大学 | A kind of fermentation culture method of fumosorosea |
| CN108770870A (en) * | 2018-06-13 | 2018-11-09 | 马瑞燕 | A kind of fumosorosea water dispersible granules and preparation method thereof |
| CN108770870B (en) * | 2018-06-13 | 2021-06-18 | 山西农业大学 | Isaria fumosorosea water dispersible granule and preparation method thereof |
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