CN101427690A - Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis - Google Patents
Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis Download PDFInfo
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- CN101427690A CN101427690A CNA2008102199936A CN200810219993A CN101427690A CN 101427690 A CN101427690 A CN 101427690A CN A2008102199936 A CNA2008102199936 A CN A2008102199936A CN 200810219993 A CN200810219993 A CN 200810219993A CN 101427690 A CN101427690 A CN 101427690A
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Abstract
The invention discloses a mixed disinsection main agent of bacillus thuringiensis and destruxins, wherein the mass ratio of destruxins to bacillus thuringiensis is 9-1:1-9. The destruxins and bacillus thuringiensis are mixed to determine the best mixing ratio, therefore the obvious synergistic action is obtained, the pesticide resistance problems of important pests of mustard family vegetables such as cabbage moth, cotton leafworm are effectively solved, with important value to control the cabbage moth and cotton leafworm and good and stable disinsection effect, strong practical application and popularization, friendly to the environment.
Description
Technical field
The invention belongs to biological pesticide technical field, be specifically related to destruxins and the Bacillus thuringiensis bacterium is used potentiating pesticidal host and preparation method thereof with.
Background technology
Destruxins (destruxin) is an insect pathogenic fungus---a kind of cyclic depsipeptides antibiotic that green muscardine fungus (Metarhiziumanisopliae var.anisopliae) secretes to the host insect haemocoele in infection processs, destruxins has significant insecticidal effect, and has the brand-new mechanism of action that is different from existing insecticide, mainly act on the hemolymph immune system of insect, make it to lose the immune defense ability.Destruxins is easily degraded, noresidue, and toxicity is low, and the development and use destruxins has important economic implications.Yet when destruxins used separately, virulence was not high enough, and control efficiency is unsatisfactory.Simultaneously, the long-term use with a kind of insecticide causes the pest resistance to insecticide development easily.Adopting the agriculture chemical compounding technology or mixing application technique is to improve the agricultural chemicals virulence and overcome or delay a drug-fast important method by synergistic effect.
(Bacillus thuringiensis, Bt) preparation is the microorganism insecticide of present world volume of production and marketing maximum to bacillus thuringiensis,Bt,, more and more comes into one's own as public nuisance-free agricultural chemicals to nontoxic, harmless, the free of contamination characteristics of higher mammal and environment with it.China is the production and application big country of Bt insecticide, has nearly 100 tame pharmaceutical factories to produce about 200 Bt formulation of insecticides, and wherein composite dose accounts for 50%.It is reported, use organophosphorus insecticides with Bt flolimat, Hostathion arranged, poison cicada with poison with synergistic effect, isocarbophos etc., pyrethroid insecticides with synergistic effect has decis, cypermethrin (beta-cypermethrin), there is the carbamates of synergistic effect that Methomyl, furans pellet etc. are arranged, no matter neires toxin is that desinsection list or dimehypo all show as remarkable synergistic effect, with other insecticides such as fluorine bell urea, fluorine worm nitrile, Imidacloprid, worm hydrazides in various degree synergistic effect is arranged all.But the pesticide resistance of insect is constantly development, and it is extremely important constantly to study new compounded preparation of pesticide, especially environmentally friendly Recompounded pesticide.
With insect diamond-back moth, prodenia litura is example, diamond-back moth (Plutella xylostella) belongs to Lepidoptera diamond-back moth section, be worldwide brassicaceous vegetable primary pest, take place throughout the year, become the important pests of the torrid zone and subtropics brassicaceous vegetable at the brassicaceous vegetable growing area.Diamond-back moth all has distribution in China each province, is the object of national pest monitoring for resistance.It is many that algebraically takes place diamond-back moth, the reproduction coefficient height, and generation overlap, the insect population quantity growth is fast, and is very serious to brassicaceous vegetable harm.Prodenia litura Spodoptera litura is the important pest of a kind of universal omnivorousness, and the frequency in China's big generation in many areas obviously improved in the last few years, and harm increases the weight of.Current chemical agent is still the main application means of the above-mentioned two kinds of insects of control, and the Bt preparation then is mainly used in pollution-free food production and organic farming.Because continuously, frequent, high dose uses; cause insect that the pesticide resistance of using chemical agent always is improved day by day; diamond-back moth has produced resistance in various degree to Multiple Pesticides such as organic phosphor, organochlorine, carbamate, pyrethroid and acyl group ureas and biologic product Bt types, and the development of drug resistance of prodenia litura is also very serious.
The pesticide resistance of insect is also in continuous development, and therefore, the technology of using with of development Bt and destruxins has practice significance for the above-mentioned two kinds of important pests of control.
Summary of the invention
An object of the present invention is to overcome existing insect to Bacillus thuringiensis bacterium pesticide resistance present situation and development destruxins pesticide technology, a kind of destruxins and the Bacillus thuringiensis bacterium desinsection host that is mixed is provided.
Purpose of the present invention realizes by following technical proposals:
The desinsection host that provides a kind of Bacillus thuringiensis bacterium and destruxins to be mixed, the mass ratio of described destruxins and Bacillus thuringiensis bacterium is 9～1: 1～9.
The mass ratio of described destruxins and Bacillus thuringiensis bacterium preferred 5～1: 5～9.
More preferred, the mass ratio of described destruxins and Bacillus thuringiensis bacterium is 5:5,4:6,3:7,2:8 or 1:9.
Described destruxins can be any among destruxins A, B or the E, perhaps the mixture of their any ratio; Described Bacillus thuringiensis bacterium (Bt) is meant that content is bacterium powder or the suitable thing of 8000IU/mg.
Described desinsection host adopts formulations such as routine techniques processing wetting powder, missible oil, microemulsion, suspending agent, granule or water dispersible granules.
The invention has the beneficial effects as follows and select destruxins and Bacillus thuringiensis bacterium to carry out composite, determined the mixed proportion that the two is best, obtained significant synergistic effect, efficiently solve the pesticide resistance problem of the important pests of brassicaceous vegetables such as diamond-back moth, prodenia litura, significant to control diamond-back moth, prodenia litura; With destruxins and Bacillus thuringiensis bacterium mixture as the insecticide good disinsection effect of host and stable, the practical application generalization is very strong, and insecticide is environmentally friendly.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.
Embodiment 1 destruxins, the single agent of Bacillus thuringiensis bacterium (Bt) and mixture thereof are to the Toxicity Determination experiment of diamond-back moth
Described destruxins can be any among destruxins A, B or the E, perhaps the mixture of their any ratio; Described Bacillus thuringiensis bacterium (Bt) is meant that content is bacterium powder or the suitable thing of 8000IU/mg.
During Toxicity Determination, adopt the blade medicine embrane method.Destruxins, Bacillus thuringiensis bacterium medicament are mixed with desired concn, and 5 concentration gradients are established in every processing, and each concentration is established 3 repetitions.In soup, flood to take out behind the 5s with a variety of Chinese cabbage blade of clean no pesticide pollution and dry in the shade, put into the culture dish that diameter 9cm is covered with filter paper, insert 15 diamond-back moth 3 instar larvaes then.Place receptacle to raise.The raising condition is 25～28 ℃ of temperature, RH70%～80%, and illumination is L: D=14h: 10h.48h check result behind 24h, other medicament medicines behind the 72h Bacillus thuringiensis bacterium medicine behind the destruxins medicine.With writing brush point touching larva, then be considered as death if health is motionless.If the blade that clear water is handled compares.Ask the regression equation and the LC of drug concentration logarithm-lethality probability value
50Value is with reference to the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun intermixture.Result of the test sees Table 1, and when destruxins and Bt were mixed with 5:5,4:6,3:7,2:8 or 1:9 ratio, its co-toxicity coefficient reached and reaches 159.58,131.77,126.17,154.20 and 136.13 respectively, has remarkable synergistic effect.
Table 1 destruxins, Bt and mixture thereof are to the Toxicity Determination of diamond-back moth
Co-toxicity coefficient CTC had the effect of subtracting effect less than 80 o'clock between the two; Greater than having synergistic effect at 120 o'clock; It in the time of between 80～120 summation action.
Embodiment 2: destruxins, the single agent of Bt and mixture thereof are to the Toxicity Determination of prodenia litura
During Toxicity Determination, measure an amount of medicament respectively, be made into certain density mother liquor with acetone and place refrigerator (4 ℃) standby, all medicaments are all used 80 solution dilutions of tween 0.01% during test.Each is handled 3 times and repeats, and each repeats 1 culture dish, each culture dish put 15 three age Spodoptera litura larvae.At first cabbage leaves is cut into the fritter of 6～7cm, takes out behind the about 5s of immersion soup, after indoor drying, again blade is put into the culture dish that is connected to prodenia litura, preserve moisture with wet cotton balls.Every 24h changes cabbage leaves and clears up culture dish behind the medicine.Experiment repeats secondary.Behind medicine 24,48,72h observed and recorded prodenia litura survival condition, calculate lethality, corrected mortality, and the lethal concentration of 50 (LC of 72h after the computing
50), correlation coefficient and regression equation, calculate the co-toxicity coefficient (CTC) of intermixture with reference to the abundant method of Sun Yun.Its result of the test sees Table 2, and destruxins mixes (except that 8:2) with Bt with any ratio all certain synergistic effect, and when the 4:6 ratio was mixed, its co-toxicity coefficient was up to 236.24, the synergistic effect highly significant.
Table 2 destruxins, Bt and mixture thereof are to the Toxicity Determination of prodenia litura
Claims (6)
1, the desinsection host that is mixed of a kind of Bacillus thuringiensis bacterium and destruxins, the mass ratio that it is characterized in that described destruxins and Bacillus thuringiensis bacterium is 9～1: 1～9.
2, the desinsection host that is mixed according to the described Bacillus thuringiensis bacterium of claim 1 and destruxins is characterized in that the mass percent preferred 5～1: 5～9 of described destruxins and Bacillus thuringiensis bacterium.
3, the desinsection host that is mixed according to the described Bacillus thuringiensis bacterium of claim 1 and destruxins, the mass ratio that it is characterized in that described destruxins and Bacillus thuringiensis bacterium is 5: 5,4: 6,3: 7,2: 8 or 1: 9.
4, the desinsection host that is mixed according to claim 1,2 or 3 described Bacillus thuringiensis bacterium and destruxins is characterized in that described destruxins is any or two or more mixtures that mix with any ratio among destruxins A, B or the E.
5, the desinsection host that is mixed according to claim 1,2 or 3 described Bacillus thuringiensis bacterium and destruxins is characterized in that described Bacillus thuringiensis bacterium is that bacterial content is bacterium powder or the suitable thing of 8000IU/mg.
6, the desinsection host that is mixed of Bacillus thuringiensis bacterium according to claim 1 and destruxins is characterized in that described desinsection host is for being processed into wetting powder, missible oil, microemulsion, suspending agent, granule or water dispersible granules.
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| CNA2008102199936A CN101427690A (en) | 2008-12-15 | 2008-12-15 | Insect disinfestation host mixed with green muscardine fungus native and Bacillus thuringiensis |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN106490011A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of biological insecticides of preventing and treating lepidoptera pest and preparation method thereof |
| CN106538623A (en) * | 2016-10-31 | 2017-03-29 | 郑州思辩科技有限公司 | A kind of plant and microorganism composite pesticide and preparation method thereof |
| CN108353899A (en) * | 2018-02-09 | 2018-08-03 | 广东省林业科学研究院 | The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis |
| CN110511883A (en) * | 2019-07-16 | 2019-11-29 | 云南省烟草公司玉溪市公司 | A kind of microbial bacterial agent and its preparation method and application for preventing and treating Spodoptera litura larvae |
| CN113508822A (en) * | 2021-05-06 | 2021-10-19 | 龙岩市农业科学研究所 | Medicament for preventing and treating phyllotreta striolata imagoes, preparation method and use method |
-
2008
- 2008-12-15 CN CNA2008102199936A patent/CN101427690A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN102687731B (en) * | 2012-06-20 | 2013-11-13 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN106490011A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of biological insecticides of preventing and treating lepidoptera pest and preparation method thereof |
| CN106538623A (en) * | 2016-10-31 | 2017-03-29 | 郑州思辩科技有限公司 | A kind of plant and microorganism composite pesticide and preparation method thereof |
| CN108353899A (en) * | 2018-02-09 | 2018-08-03 | 广东省林业科学研究院 | The compounded pesticides and its application of green muscardine fungus and Empedobacter brevis |
| CN110511883A (en) * | 2019-07-16 | 2019-11-29 | 云南省烟草公司玉溪市公司 | A kind of microbial bacterial agent and its preparation method and application for preventing and treating Spodoptera litura larvae |
| CN113508822A (en) * | 2021-05-06 | 2021-10-19 | 龙岩市农业科学研究所 | Medicament for preventing and treating phyllotreta striolata imagoes, preparation method and use method |
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Open date: 20090513 |