CN106538623A - A kind of plant and microorganism composite pesticide and preparation method thereof - Google Patents
A kind of plant and microorganism composite pesticide and preparation method thereof Download PDFInfo
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- CN106538623A CN106538623A CN201610931380.XA CN201610931380A CN106538623A CN 106538623 A CN106538623 A CN 106538623A CN 201610931380 A CN201610931380 A CN 201610931380A CN 106538623 A CN106538623 A CN 106538623A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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Abstract
The invention discloses a kind of plant and microorganism composite pesticide, it is characterised in that：Including the raw material of following parts by weight：20 30 parts of Radix Aconiti, 20 30 parts of Cynanchum Komarovii, 10 15 parts of Herba Pogostemonis, 10 15 parts of Herba Artemisiae annuae, 8 12 parts of Radix Polygoni Multiflori, 48 parts of bacillus thuringiensiss powder, 24 parts of green muscardine funguss powder, 70 80 parts of carrier, 5 12 parts of wetting agent, 25 parts of dispersant.The raw material of the pesticide is plant material and microorganism raw material compounding use, can be had complementary advantages, parasite killing spectrum width, and insecticidal effect is notable, noresidue, without Drug resistance, environmental protection.
Description
Technical field
The invention belongs to field of biological pesticide, more particularly, to a kind of plant and microorganism composite pesticide and its preparation side
Method.
Background technology
Vegetable is the important industrial crops of China, and vegetables industry is played in increasing peasant income, growth of agricultural efficiency and rural development
Highly important effect, vegetable-crop pest-insect cause harm be cause Quality and biomass of vegetables decline principal element, common evil in vegetable
Worm has Pieris rapae, diamondback moth, beet armyworm, Prodenia litura, aleyrodid, aphid, thrips and tetranychid etc..These insects are by taking food
Plant leaf and affect vegetable normal growth, it is also possible to by brill moth so as to having a strong impact on the quality and market value of vegetable.Mesh
The Main Means of front preventing and treating vegetable-crop pest-insect are to use chemical pesticide, such as：Phoxim, pyridalyl and Acetamiprid etc., Jing often makes
Increasingly sharpened the development of such pest resistance to insecticide with chemical pesticide, increased pests control cost and difficulty, is also resulted in tight
The food and environment pesticide residues of weight, the residual exceeded phenomenon of vegetable agriculture are also very serious, therefore are badly in need of developing new and effective safe
Biological pesticide.
Chinese Patent Application No. 200510114684.9 discloses a kind of fructus cnidii thuricin complex insecticide, should
Insecticide can be used to prevent and treat lepidoptera pest, the invention provides osthole（A）With Bacillus thuringiensis（B）The ratio model of compounding
Enclose for 1:1-60, more preferably 1:1-25, is respectively provided with the work of potentiation to lepidopteran insects such as diamondback moth, Pieris rapae after both compoundings
With.The invention osthole and Bacillus thuringiensis, can play certain potentiation, but only with osthole, lead
Cause the insecticidal spectrum of the insecticide narrow.
Chinese Patent Application No. 201610283959.X discloses a kind of vegetable green pesticide and preparation method thereof, institute
Stating vegetable green pesticide includes according to the raw material of weight portion：It is the Radix Pulsatillae, Rhizoma Zingiberis Recenss, Rhizoma Coptidis, Radix Agrimoniae, Radix Angelicae Pubescentiss, the Rhizoma Pinelliae, deep
Grass, Herba Menthae, Radix Gentianae, essential balm, potassium permanganate, Pericarpium Zanthoxyli, Herba Pogostemonis, Herba Gelsemii Elegantis, stomach mollugo, Herba Solani Nigri, trade route, Flos Daturae, Radix Aconiti Kusnezoffii
Head, the Radix Stemonae, Radix Tripterygii Wilfordii, Pyrethrum, Radix Arnebiae (Radix Lithospermi), soapberry fruit, pomelo peel, Nicotiana tabacum L., Flos Rhododendri Mollis, Fructus Carpesii, Semen Arecae, Semen Torreyae.The invention
Prepare for pure Chinese herbal medicine raw material, during use carrier or other compositions to compound, high cost, toxicity are big.
The content of the invention
In view of this, the purpose of the present invention is for the deficiencies in the prior art, there is provided a kind of plant and the compound agriculture of microorganism
Medicine, the raw material of the pesticide is plant material and microorganism raw material compounding use, can be had complementary advantages, and parasite killing spectrum width, insecticidal effect show
Write, noresidue, without Drug resistance, environmental protection.
In addition the invention also discloses the preparation method of the plant and microorganism composite pesticide.
To reach above-mentioned purpose, the present invention is employed the following technical solutions：
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：Radix Aconiti 20-30 parts, Cynanchum Komarovii 20-30
Part, Herba Pogostemonis 10-15 parts, Herba Artemisiae annuae 10-15 parts, Radix Polygoni Multiflori 8-12 parts, bacillus thuringiensiss powder 4-8 parts, green muscardine funguss powder 2-4
Part, carrier 70-80 parts, wetting agent 5-12 parts, dispersant 2-5 parts.
Further, the bacillus thuringiensiss powder and the weight ratio of green muscardine funguss powder are 2:1.
Further, in the bacillus thuringiensiss powder bacillus thuringiensiss content be 32000IU/mg, green deadlock
In mycopowder, the content of green muscardine funguss is 50,000,000,000/g.
Further, the carrier is the one kind in Kaolin, bentonite, white carbon or attapulgite.
Further, the wetting agent is the one kind in Morwet EFW, sodium lauryl sulphate, Propylene Glycol or glycerol.
Further, the dispersant be sodium lignin sulfonate, dodecylbenzene sodium sulfonate, alkylphenol polyoxyethylene or
One kind in Polysorbate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 80-100 mesh is crushed to, Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Ethanol solution of the 6-10 times of bulking value fraction for 70-80%, while adding Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount 0.1-0.2
The hemicellulase of times weight, soaks 2-3h, and then ultrasonic extraction 2-3 time, merging filtrate are evaporated to without alcohol taste, obtain
Extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 40-80 mesh is crushed to, Herba Pogostemonis and Herba Artemisiae annuae total amount 6-12 times bulking value is added
Ethanol solution of the fraction for 70-80%, then in apparatus,Soxhlet'ses, water-bath reflux, extract, 3-6h, filtrate reduced in volume is to without alcohol
Taste, obtains extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after oven drying at low temperature, 500-600 mesh is crushed to, pesticide is prepared.
Further, the step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Further, the step（1）The power of middle ultrasonic extraction is 400-700W, and extraction time is 30-50min.
Further, the step（3）The temperature of middle oven drying at low temperature is 40-50 DEG C.
The invention has the beneficial effects as follows：
1st, the raw material that the plant and microorganism composite pesticide that prepared by the present invention adopts is that plant material and microorganism raw material are compounded.Its
Middle plant material includes Radix Aconiti, Cynanchum Komarovii, Herba Pogostemonis, Herba Artemisiae annuae and Radix Polygoni Multiflori, and wherein the aconite alkaloid in Radix Aconiti is to evil
Worm has the effect of very strong stomach poison function, Antifeedant Effects and biological inhibition；Alkaloid in Cynanchum Komarovii has very strong tagging
Effect；Pogostone in Herba Pogostemonis, is also tagged with very strong Antifeedant Effects in addition to insect, growth inhibited and ovicidal are made
With；Volatile oil and arteannuin in Herba Artemisiae annuae has very strong stifling and action of contace poison to insect；And the rheum emodin in Radix Polygoni Multiflori
Middle Anthraquinones and phospholipid class pest have very strong growth inhibited；This several raw material have concurrently stomach toxicity, tag, refusing to eat, it is stifling and
Growth inhibition effect, can widen insecticidal spectrum with Synergistic insecticidal, and insecticidal rate is faster.
Microorganism raw material includes bacillus thuringiensiss powder and green muscardine funguss powder, the wherein Su Yun in bacillus thuringiensiss powder
The crystalline toxin that golden bacillus cereuss are produced has strong insecticidal action, but generally existing in actual use to diamondback moth
The problems such as virulence is not strong, insecticidal spectrum is narrow, the longevity of residure is short, effect is slow；And green muscardine funguss are that a kind of parasitic scope is wide in green muscardine funguss powder
Insect pathogenic fungus, can by contaminate insect body wall or digestive tract make insect lethal, but insecticidal rate compares
And effect is weaker slowly,.Bacillus thuringiensiss and green muscardine funguss mixture are used, can increase to a certain extent parasite killing timeliness and
Insecticidal effect, expands insecticidal spectrum, reduces Drug resistance.Jing present invention checkings, bacillus thuringiensiss and green muscardine funguss by weight 2:1
During mixture, insecticidal effect is best.
By plant material and microorganism raw material compounding use, desinsection approach can be increased, reduce Drug resistance, collaboration in addition is killed
Worm, it is also possible to increase the insecticidal effect of insecticide, improves insecticidal rate, expands insecticidal spectrum.
2nd, the present invention is different according to plant material active insecticidal components, using different extracting method.Wherein in Radix Aconiti
Main insecticidal constituent is aconite alkaloid, and the main insecticidal constituent in Cynanchum Komarovii is alkaloid, the main parasite killing in Radix Polygoni Multiflori
Composition is Anthraquinones and phospholipid, therefore to these three raw materials of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori using ethanol ultrasonic extraction
Method, also using hemicellulase enzymolysis destruction plant cell wall, makes the composition in plant cell be more easy to dissolution, to increase extraction
Rate.And the main insecticidal constituent in Herba Pogostemonis and Herba Artemisiae annuae is volatile oil, using the method for alcohol reflux, volatile component is damaged
Lose little, extraction ratio height.
In addition, when plant is prepared with microorganism composite pesticide, extract I and extraction II are mixed with carrier, and of the invention
With Kaolin, bentonite, white carbon and attapulgite as carrier, this several carrier inner ratio surface area than larger, can by activity into
Divide locking in the carrier, reduce the loss of active component in preparation process, good disinsection effect when actually used.
3rd, the present invention can increase pesticide with Morwet EFW, sodium lauryl sulphate, Propylene Glycol or glycerol as wetting agent
Water solublity so that pesticide easily disperses, good disinsection effect.With sodium lignin sulfonate, dodecylbenzene sodium sulfonate, alkyl
Phenol polyethenoxy ether or Polysorbate are dispersant, active component can be made to disperse in the carrier evenly, so as to reduce activity
Component damages.
4th, pesticide of the invention is 91.7%-98.3% to the corrected mortality of Pieris rapae, the corrected mortality to diamondback moth
For 91.7%-100%, significantly, insecticidal effect is notable, and noresidue, without Drug resistance, environmental protection for insecticidal effect.
Specific embodiment
With reference to specific embodiment, the invention will be further described, wherein the original adopted in each embodiment of the invention
Material Radix Aconiti, Cynanchum Komarovii, Herba Pogostemonis, Herba Artemisiae annuae and Radix Polygoni Multiflori are the raw material of fresh drying.
Embodiment 1
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 20 parts of Radix Aconiti, 20 parts of Cynanchum Komarovii, wide
15 parts of Herba Pogostemoniss, 15 parts of Herba Artemisiae annuae, 8 parts of Radix Polygoni Multiflori, 4 parts of bacillus thuringiensiss powder, 2 parts of green muscardine funguss powder, 70 parts of carrier, wetting agent
5 parts, 5 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is Kaolin.
Wherein wetting agent is Morwet EFW.
Wherein dispersant is sodium lignin sulfonate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 80 mesh are crushed to, 6 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 70% ethanol solution, at the same add Radix Aconiti, 0.1 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 2h, and then ultrasonic extraction 2 times, the wherein power of ultrasonic extraction are 400W, and extraction time is 30min, so
Merging filtrate, is evaporated to without alcohol taste afterwards, obtains extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 40 mesh are crushed to, addition 6 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
70% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 3h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 40 DEG C of oven drying at low temperatures, 500 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 2
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 22 parts of Radix Aconiti, 22 parts of Cynanchum Komarovii, wide
14 parts of Herba Pogostemoniss, 14 parts of Herba Artemisiae annuae, 9 parts of Radix Polygoni Multiflori, 5 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 72 parts of carrier, wetting agent
6 parts, 4 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is bentonite.
Wherein wetting agent is sodium lauryl sulphate.
Wherein dispersant is dodecylbenzene sodium sulfonate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 90 mesh are crushed to, 7 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 72% ethanol solution, at the same add Radix Aconiti, 0.2 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 2h, and then ultrasonic extraction 3 times, the wherein power of ultrasonic extraction are 500W, and extraction time is 40min, so
Merging filtrate, is evaporated to without alcohol taste afterwards, obtains extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 50 mesh are crushed to, addition 7 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
72% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 4h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 45 DEG C of oven drying at low temperatures, 600 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 3
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 24 parts of Radix Aconiti, 24 parts of Cynanchum Komarovii, wide
13 parts of Herba Pogostemoniss, 13 parts of Herba Artemisiae annuae, 10 parts of Radix Polygoni Multiflori, 6 parts of bacillus thuringiensiss powder, 4 parts of green muscardine funguss powder, 74 parts of carrier, moistening
7 parts of agent, 3 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is white carbon.
Wherein wetting agent is Propylene Glycol.
Wherein dispersant is alkylphenol polyoxyethylene.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 100 mesh are crushed to, 8 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 75% ethanol solution, at the same add Radix Aconiti, 0.15 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 3h, and then ultrasonic extraction 2 times, the wherein power of ultrasonic extraction are 600W, and extraction time is 50min, so
Merging filtrate, is evaporated to without alcohol taste afterwards, obtains extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 60 mesh are crushed to, addition 8 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
75% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 5h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 50 DEG C of oven drying at low temperatures, 600 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 4
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 25 parts of Radix Aconiti, 25 parts of Cynanchum Komarovii, wide
12 parts of Herba Pogostemoniss, 12 parts of Herba Artemisiae annuae, 11 parts of Radix Polygoni Multiflori, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 75 parts of carrier, moistening
8 parts of agent, 3 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is attapulgite.
Wherein wetting agent is Propylene Glycol.
Wherein dispersant is alkylphenol polyoxyethylene.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 90 mesh are crushed to, 9 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 75% ethanol solution, at the same add Radix Aconiti, 0.15 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 2.5h, and then ultrasonic extraction 3 times, the wherein power of ultrasonic extraction are 600W, and extraction time is 40min,
Filtrate is then combined with, is evaporated to without alcohol taste, obtain extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 70 mesh are crushed to, addition 9 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
75% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 5h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 45 DEG C of oven drying at low temperatures, 500 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 5
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 25 parts of Radix Aconiti, 25 parts of Cynanchum Komarovii, wide
12 parts of Herba Pogostemoniss, 12 parts of Herba Artemisiae annuae, 11 parts of Radix Polygoni Multiflori, 11 parts of Cortex Meliae, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, load
75 parts of body, 8 parts of wetting agent, 3 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is attapulgite.
Wherein wetting agent is Propylene Glycol.
Wherein dispersant is alkylphenol polyoxyethylene.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Weigh Radix Aconiti, Cynanchum Komarovii, Cortex Meliae and Radix Polygoni Multiflori, be crushed to 90 mesh, add Radix Aconiti, Cynanchum Komarovii, Cortex Meliae and
9 times of bulking value fractions of Radix Polygoni Multiflori total amount are 75% ethanol solution, while adding Radix Aconiti, Cynanchum Komarovii, Cortex Meliae and Radix Polygoni Multiflori
The hemicellulase of 0.15 times of weight of total amount, soaks 2.5h, then ultrasonic extraction 3 times, and the power of wherein ultrasonic extraction is
600W, extraction time are 40min, are then combined with filtrate, are evaporated to without alcohol taste, obtain extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 70 mesh are crushed to, addition 9 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
75% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 5h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 45 DEG C of oven drying at low temperatures, 500 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 6
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 26 parts of Radix Aconiti, 26 parts of Cynanchum Komarovii, wide
11 parts of Herba Pogostemoniss, 11 parts of Herba Artemisiae annuae, 12 parts of Radix Polygoni Multiflori, 7 parts of bacillus thuringiensiss powder, 2 parts of green muscardine funguss powder, 78 parts of carrier, moistening
10 parts of agent, 2 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is Kaolin.
Wherein wetting agent is glycerol.
Wherein dispersant is Polysorbate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 80 mesh are crushed to, 10 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 80% ethanol solution, at the same add Radix Aconiti, 0.1 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 2h, and then ultrasonic extraction 2 times, the wherein power of ultrasonic extraction are 700W, and extraction time is 50min, so
Merging filtrate, is evaporated to without alcohol taste afterwards, obtains extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 80 mesh are crushed to, addition 10 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
80% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 6h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 50 DEG C of oven drying at low temperatures, 600 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 7
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 28 parts of Radix Aconiti, 28 parts of Cynanchum Komarovii, wide
10 parts of Herba Pogostemoniss, 10 parts of Herba Artemisiae annuae, 8 parts of Radix Polygoni Multiflori, 8 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 80 parts of carrier, wetting agent
11 parts, 3 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is bentonite.
Wherein wetting agent is glycerol.
Wherein dispersant is sodium lignin sulfonate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 100 mesh are crushed to, 8 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 70% ethanol solution, at the same add Radix Aconiti, 0.2 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 3h, and then ultrasonic extraction 3 times, the wherein power of ultrasonic extraction are 500W, and extraction time is 40min, so
Merging filtrate, is evaporated to without alcohol taste afterwards, obtains extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 50 mesh are crushed to, addition 11 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
80% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 6h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 40 DEG C of oven drying at low temperatures, 600 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Embodiment 8
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 30 parts of Radix Aconiti, 30 parts of Cynanchum Komarovii, wide
12 parts of Herba Pogostemoniss, 12 parts of Herba Artemisiae annuae, 9 parts of Radix Polygoni Multiflori, 8 parts of bacillus thuringiensiss powder, 4 parts of green muscardine funguss powder, 80 parts of carrier, wetting agent
12 parts, 4 parts of dispersant.
Wherein in bacillus thuringiensiss powder the content of bacillus thuringiensiss be 32000IU/mg, green deadlock in green muscardine funguss powder
The content of bacterium is 50,000,000,000/g.
Wherein carrier is attapulgite.
Wherein wetting agent is Morwet EFW.
Wherein dispersant is dodecylbenzene sodium sulfonate.
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 90 mesh are crushed to, 9 times of Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Bulking value fraction is 75% ethanol solution, at the same add Radix Aconiti, 0.2 times of weight of Cynanchum Komarovii and Radix Polygoni Multiflori total amount it is half fine
The plain enzyme of dimension, soaks 2.5h, and then ultrasonic extraction 3 times, the wherein power of ultrasonic extraction are 600W, and extraction time is 30min,
Filtrate is then combined with, is evaporated to without alcohol taste, obtain extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 60 mesh are crushed to, addition 12 times of bulking value fractions of Herba Pogostemonis and Herba Artemisiae annuae total amount are
70% ethanol solution, then in apparatus,Soxhlet'ses, 100 DEG C of water-bath reflux, extract, 3h, filtrate reduced in volume are obtained to without alcohol taste
Extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after 45 DEG C of oven drying at low temperatures, 500 mesh are crushed to, pesticide is prepared.
Wherein step（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
Comparative example 1
Comparative example 1 is substantially the same manner as Example 4, and difference is：Raw material is adjusted, and raw material total amount keeps constant,
Radix Aconiti in raw material is removed, the amount of Cynanchum Komarovii is accordingly increased, i.e.,：
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：50 parts of Cynanchum Komarovii, 12 parts of Herba Pogostemonis,
12 parts of Herba Artemisiae annuae, 11 parts of Radix Polygoni Multiflori, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 75 parts of carrier, 8 parts of wetting agent, dispersion
3 parts of agent.
Its preparation method is same as Example 4.
Comparative example 2
Comparative example 2 is substantially the same manner as Example 4, and difference is：Raw material is adjusted, and raw material total amount keeps constant,
Cynanchum Komarovii in raw material is removed, the amount of Radix Aconiti is accordingly increased, i.e.,：
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：50 parts of Radix Aconiti, 12 parts of Herba Pogostemonis, Hemerocallis citrina Baroni
12 parts of Artemisia, 11 parts of Radix Polygoni Multiflori, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 75 parts of carrier, 8 parts of wetting agent, dispersant 3
Part.
Its preparation method is same as Example 4.
Comparative example 3
Comparative example 3 is substantially the same manner as Example 4, and difference is：Raw material is adjusted, and raw material total amount keeps constant,
Herba Pogostemonis in raw material and Herba Artemisiae annuae are removed, the amount of Radix Aconiti and Cynanchum Komarovii is accordingly increased, i.e.,：
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：37 parts of Radix Aconiti, 37 parts of Cynanchum Komarovii, what
11 parts of the Radix Polygoni Multiflori, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 75 parts of carrier, 8 parts of wetting agent, 3 parts of dispersant.
Its preparation method is same as Example 4.
Comparative example 4
Comparative example 4 is substantially the same manner as Example 4, and difference is：Raw material is adjusted, and raw material total amount keeps constant,
Radix Polygoni Multiflori in raw material is removed, the amount of Herba Artemisiae annuae is accordingly increased, i.e.,：
A kind of plant and microorganism composite pesticide, including the raw material of following parts by weight：It is 25 parts of Radix Aconiti, 25 parts of Cynanchum Komarovii, wide
12 parts of Herba Pogostemoniss, 23 parts of Herba Artemisiae annuae, 6 parts of bacillus thuringiensiss powder, 3 parts of green muscardine funguss powder, 75 parts of carrier, 8 parts of wetting agent, dispersant
3 parts.
Its preparation method is same as Example 4.
Comparative example 5
The raw material that comparative example 5 is adopted with embodiment 4 is identical, and difference is that the preparation method of pesticide has been adjusted, only with
Ultrasonic extraction, i.e.,：
A kind of plant and the preparation method of microorganism composite pesticide, comprise the following steps：
（1）Raw material Radix Aconiti, Cynanchum Komarovii, Radix Polygoni Multiflori, Herba Pogostemonis and Herba Artemisiae annuae are weighed, 90 mesh are crushed to, 9 times of bulking values are added
Fraction is 75% ethanol solution, while adding the hemicellulase of 0.15 times of weight, soaks 2.5h, then ultrasonic extraction 3
Secondary, wherein the power of ultrasonic extraction is 600W, and extraction time is 40min, is then combined with filtrate, is evaporated to without alcohol taste,
Obtain extract；
（2）After the extract, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant uniform,
After 45 DEG C of oven drying at low temperatures, 500 mesh are crushed to, pesticide is prepared.
Plant is tested with microorganism composite pesticide indoor virulence
The product prepared in embodiment of the present invention 1-8 and comparative example 1-5 is dissolved in 1000 times of water, as supplying reagent
Liquid, respectively using Pieris rapae and diamondback moth as confession examination insect.Specifically test method is：By fresh Plantula Brassicae chinensis blade for reagent liquid
Middle dipping 5s, dries naturally, then will impregnate 5s for trying insect in for reagent liquid, is absorbed with absorbent paper and is remained in for examination insect table
The medicinal liquid in face, then will together be placed in culture dish for trying insect and Plantula Brassicae chinensis, place 10 for examination evil in each culture dish
The respectively test 60 of worm, wherein Pieris rapae and diamondback moth, then culture dish prick upper gauze, it is artificial for 26 ± 1 DEG C to be placed on temperature
Cultivating in weather incubator, dead borer population being checked after 12h, wherein polypide is stirred with brush pen, polypide is motionless to be judged to death,
Per group of test is repeated 3 times, and the confession reagent liquid that the controlled trial in the present invention is used is water.
Mortality rate（%）=dead borer population/total borer population × 100%
Corrected mortality（%）=（Process mortality rate-control mortality rate）/（1- compares mortality rate）×100%
1 insecticide indoor virulence test data of table
As can be seen from Table 1, correction of the plant that prepared by embodiment of the present invention 1-8 with microorganism composite pesticide to Pieris rapae is dead
Rate is 91.7%-98.3%, is 91.7%-100% to the corrected mortality of diamondback moth, and insecticidal effect is notable, and noresidue, nonreactive
The property of medicine.
Embodiment 5 is substantially the same manner as Example 4, and it is on the basis of embodiment 4 to increased one that difference is embodiment 5
Raw material Cortex Meliae is planted, and the Toosendanin in Cortex Meliae has very strong toxic action to insect, therefore increase the embodiment 5 of Cortex Meliae
Effect be better than embodiment 4, illustrate Cortex Meliae also with Synergistic insecticidal effect, but toxicity is also bigger than normal.
Comparative example 1-4 is raw material to be adjusted on the basis of embodiment 4, and raw material total amount is constant, preparation method phase
Together.Wherein the Radix Aconiti in raw material is removed by comparative example 1, accordingly increases the amount of Cynanchum Komarovii；Comparative example 2 is by the Cor Bovis seu Bubali in raw material
Piao Zi removes, and accordingly increases the amount of Radix Aconiti；Herba Pogostemonis in raw material and Herba Artemisiae annuae are removed by comparative example 3, accordingly increase river
Crow and the amount of Cynanchum Komarovii；Radix Polygoni Multiflori in raw material is removed by comparative example 4, accordingly increases the amount of Herba Artemisiae annuae；And comparative example 1-4
The pesticide of preparation is lower than embodiment 4 to Pieris rapae and diamondback moth corrected mortality, illustrates this several raw material of present invention selection, excellent
Gesture is complementary, and the effect with Synergistic each other lacks any one, and insecticidal effect accordingly will weaken.
Comparative example 5 is that preparation method is have adjusted on the basis of embodiment 4, does not do classification and extracts, only with supersound extraction
Method, and comparative example 5 prepare pesticide it is lower than embodiment 4 to the corrected mortality of Pieris rapae and diamondback moth, illustrate according to original
Material characteristic carries out classification extraction, can strengthen the extraction ratio of insecticidal constituent, reduces loss, so as to strengthen insecticidal effect.
Finally illustrate, only to illustrate technical scheme and unrestricted, this area is common for above example
Other modifications or equivalent that technical staff is made to technical scheme, without departing from technical solution of the present invention
Spirit and scope, all should cover in the middle of scope of the presently claimed invention.
Claims (10)
1. a kind of plant and microorganism composite pesticide, it is characterised in that：Including the raw material of following parts by weight：Radix Aconiti 20-30 parts,
Cynanchum Komarovii 20-30 parts, Herba Pogostemonis 10-15 parts, Herba Artemisiae annuae 10-15 parts, Radix Polygoni Multiflori 8-12 parts, bacillus thuringiensiss powder 4-8
Part, green muscardine funguss powder 2-4 parts, carrier 70-80 parts, wetting agent 5-12 parts, dispersant 2-5 parts.
2. a kind of plant according to claim 1 and microorganism composite pesticide, it is characterised in that：The Su Yun gold spore bar
Mycopowder is 2 with the weight ratio of green muscardine funguss powder:1.
3. a kind of plant according to claim 1 and microorganism composite pesticide, it is characterised in that：The Su Yun gold spore bar
In mycopowder, the content of bacillus thuringiensiss is 32000IU/mg, and in green muscardine funguss powder, the content of green muscardine funguss is 50,000,000,000/g.
4. a kind of plant according to claim 1 and microorganism composite pesticide, it is characterised in that：The carrier is kaolinite
One kind in soil, bentonite, white carbon or attapulgite.
5. a kind of plant according to claim 1 and microorganism composite pesticide, it is characterised in that：The wetting agent is
One kind in Morwet EFW, sodium lauryl sulphate, Propylene Glycol or glycerol.
6. a kind of plant according to claim 1 and microorganism composite pesticide, it is characterised in that：The dispersant is wooden
One kind in plain sodium sulfonate, dodecylbenzene sodium sulfonate, alkylphenol polyoxyethylene or Polysorbate.
7. the preparation method of a kind of plant as claimed in claim 1 and microorganism composite pesticide, it is characterised in that：Including following
Step：
（1）Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori are weighed, 80-100 mesh is crushed to, Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount is added
Ethanol solution of the 6-10 times of bulking value fraction for 70-80%, while adding Radix Aconiti, Cynanchum Komarovii and Radix Polygoni Multiflori total amount 0.1-0.2
The hemicellulase of times weight, soaks 2-3h, and then ultrasonic extraction 2-3 time, merging filtrate are evaporated to without alcohol taste, obtain
Extract I；
（2）Herba Pogostemonis and Herba Artemisiae annuae are weighed, 40-80 mesh is crushed to, Herba Pogostemonis and Herba Artemisiae annuae total amount 6-12 times bulking value is added
Ethanol solution of the fraction for 70-80%, then in apparatus,Soxhlet'ses, water-bath reflux, extract, 3-6h, filtrate reduced in volume is to without alcohol
Taste, obtains extract II；
（3）The extract I, extract II, bacillus thuringiensiss powder, green muscardine funguss powder, carrier, wetting agent and dispersant are mixed
After closing uniformly, after oven drying at low temperature, 500-600 mesh is crushed to, pesticide is prepared.
8. the preparation method of plant according to claim 7 and microorganism composite pesticide, it is characterised in that：The step
（1）Middle hemicellulase enzyme activity is 3 × 104U/g。
9. the preparation method of plant according to claim 7 and microorganism composite pesticide, it is characterised in that：The step
（1）The power of middle ultrasonic extraction is 400-700W, and extraction time is 30-50min.
10. the preparation method of plant according to claim 7 and microorganism composite pesticide, it is characterised in that：The step
（3）The temperature of middle oven drying at low temperature is 40-50 DEG C.
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Application publication date: 20170329 |