KR20130011644A - Composition for controlling lawn-disease comprising plant extracts and vegetable oils as effective components - Google Patents

Composition for controlling lawn-disease comprising plant extracts and vegetable oils as effective components Download PDF

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KR20130011644A
KR20130011644A KR1020110072944A KR20110072944A KR20130011644A KR 20130011644 A KR20130011644 A KR 20130011644A KR 1020110072944 A KR1020110072944 A KR 1020110072944A KR 20110072944 A KR20110072944 A KR 20110072944A KR 20130011644 A KR20130011644 A KR 20130011644A
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polyoxyethylene
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전한택
김명진
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전한택
김명진
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/22Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/30Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Dispersion Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

PURPOSE: A composition containing a plant extract and vegetable oil is provided to save time for culturing microorganisms and to prevent turf disease without harm to humans. CONSTITUTION: A composition for preventing turf diseases contains: a plant extracts prepared from Portulaca oleracea, Echinacea, mangosteen, Curcumae longae Rhizoma, Achyranthes radical, Coptidis Rhizoma, tangerine, Phellodendri Cortex, pomegranate, Curcumae longae Rhizoma, pine, Rhei Rhizoma, Scutellariae Radix, Phytolacca acinosa, Polygonum multiflorum, Chinese dioscorea, Aristolochia contorta, garlic, Thea Sinensis Linn, Cephalanoplos segetum, Melandrium firmum, Kalopanax pictus Nakai, sarracenia, ginkgo biloba, Tartarian aster, Chrysanthemi Flos, Gentianae scabrae Radix, or Scrophulariae Radix; and vegetable oil prepared from lemongrass, lavender, star anise, mandarin, oregano, rosegeranium, tea tree, clove leaf, canola, castor, or neem.

Description

식물추출물 및 식물성 오일을 유효성분으로 포함하는 잔디 병해 방제용 조성물{Composition for Controlling Lawn-Disease Comprising Plant Extracts and Vegetable Oils as Effective Components}Composition for Controlling Lawn Diseases Comprising Plant Extracts and Vegetable Oils as Active Ingredients

본 발명은 마치현(Portulaca oleracea), 에키나시아(Echinacea), 소리쟁이, 망고스틴(Mangosteen), 강황, 지치, 쑥, 도꼬마리, 우슬근, 황련, 감귤, 자소, 무환자나무, 황백, 석류, 울금, 소나무, 대황, 애기똥풀, 매운냉이, 민족두리풀, 황금, 자리공, 마디풀, 산약, 쥐방울덩굴, 맥문동, 마늘, 차나무, 제비꽃, 조릿대, 조뱅이, 장구채, 닭의 장풀, 이질풀, 달래, 음나무, 끈끈이주걱, 은행나무, 꽈리, 인동덩굴, 깽깽이풀, 개미취, 개암풀, 감국, 용담, 가락풀나무, 오이풀, 질경이, 목향, 방풍, 측백나무, 백선, 한련초, 비파나무, 할미꽃, 산초나무, 현삼, 소태나무, 환삼덩굴, 협죽도, 형개, 홀아비꽃대 및 담쟁이덩굴로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물추출물; 및 레몬그라스(Lemongrass), 라벤더(Lavender), 스타아니스(Star anise), 만다린(Mandarin), 타임(Thyme), 잣나무, 오레가노(Oregano), 로즈제라늄(Rosegeranium), 티트리(Tea tree), 클로브리프(Clove leaf), 캐놀라(Canola), 피마자(Castor) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물성 오일을 유효성분으로 포함하는 잔디 병해 방제용 조성물에 관한 것이다.
The present invention is Portulaca oleracea, Echinacea, Sounder, Mangosteen, Turmeric, Chichi, Mugwort, Beetweed, Hyssopmus, Rhododendron, Citrus fruits, Jasmine, Unripe tree, Yellow white, Pomegranate, Turmeric, Pine , Rhubarb, celandine, hotcress, horseradish, golden, percussion, knotweed, potion, rattan, ganmundong, garlic, tea tree, violet, stalk, zinnia, jangseok, chicken's grass, cranesbill, soothing, vine, stick spatula, bank Tree, cherry tree, honeysuckle, honeysuckle, anthill, hazelnut, country, gentian, nectar, cucumber grass, plantain, cedar, windproof, cypress, ringworm, nasturtium, loquat, pasqueflower, japanese herb, hyunsam, pine Plant extracts obtained by extracting from any one or more selected from the group consisting of hwansam vine, oleander, hyungae, widower and ivy; And Lemongrass, Lavender, Star anise, Mandarin, Thyme, Pine, Oregano, Roseegeranium, Tea tree, Claw It relates to a grass disease control composition comprising a vegetable oil obtained by extracting from at least one selected from the group consisting of Clove leaf, Canola, Canola, Castor and Nim as an active ingredient.

잔디는 여러해살이풀로 재생력이 강하고 항상 푸르기 때문에 공원 및 화단에 조경을 위해 많이 사용되며, 골프장이나 운동경기장에도 많이 이용되고 있다. 일반적으로 잔디는 자생조건에 관계없이 인위적으로 심어진 것이기 때문에 특별한 관리를 하지 않으면 많은 병해가 발생되어 피해를 입게 된다. 따라서, 잔디의 생장을 위해 관리자들은 농약 및 제초제를 뿌려 관리하는데, 이는 토양오염 및 지하수오염을 유발하는 문제점이 발생한다. The grass is a perennial plant and is used for landscaping in parks and flowerbeds because it is rejuvenated and is always green. It is also used for golf and sports fields. In general, lawns are artificially planted regardless of their native conditions, so many special diseases are caused and damages occur without special management. Therefore, managers spray and manage pesticides and herbicides for grass growth, which causes soil pollution and groundwater pollution.

따라서, 잔디 병해 방제를 위한 친환경적인 방법의 중요성이 부각되고 있으며, 친환경적인 방제를 위해 미생물이나 바이러스를 이용하는 생물농약이 상품화되고 있다. 생물농약에 이용되는 미생물로는 바실러스 서브틸리스(Bacillus subtilis), 바실러스 프밀러스(Bacillus pumilus), 바실러스 리케니포르미스(Bacillus licheniformis), 스트렙토마이세스속(Streptomyces sp.), 아그로박테리움속(Agrobacteriom sp.) 및 슈도모나스속(Pseudomona sp.) 등이 있으며 국내외적으로 많은 연구가 진행되어 왔다. Therefore, the importance of environmentally friendly methods for grass disease control is emerging, and bio pesticides using microorganisms or viruses are being commercialized for environmentally friendly control. Microorganisms used in biopesticides include Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, Streptomyces sp. And Agrobacterium sp. Agrobacteriom sp.) And Pseudomona sp., And many studies have been conducted at home and abroad.

하지만, 미생물은 상호 길항작용이 없는 미생물끼리 혼합하여야 하며, 하나의 미생물을 이용할 경우 방제 효과가 나타나려면 미생물이 토양에서 정착한 후 성장하여 병원균을 억제할 수 있는 물질을 분비하는데 오랜 시간이 소요된다. 또한, 제품내에 병원균 억제 산물이 포함되어 있는 경우는 희석배수가 약하여 현장에 적용하기 힘들다는 문제점이 있다. 또한, 대량생산과 보존을 하기 힘들다는 문제점이 있다.
However, the microorganisms should be mixed with each other without mutual antagonism, and if one microorganism is used, it takes a long time to release a substance capable of inhibiting pathogens by growing after the microorganisms settle in the soil. . In addition, when the product contains a pathogen suppression product, there is a problem that it is difficult to apply to the field because the dilution drainage is weak. In addition, there is a problem that it is difficult to mass production and preservation.

대한민국 공개특허공보 제10-2009-0104337호Republic of Korea Patent Publication No. 10-2009-0104337 대한민국 공개특허공보 제10-2010-0070265호Republic of Korea Patent Publication No. 10-2010-0070265

이에, 본 발명자들은 마치현(Portulaca oleracea), 에키나시아(Echinacea), 소리쟁이, 망고스틴(Mangosteen), 강황, 지치, 쑥, 도꼬마리, 우슬근, 황련, 감귤, 자소, 무환자나무, 황백, 석류, 울금, 소나무, 대황, 애기똥풀, 매운냉이, 민족두리풀, 황금, 자리공, 마디풀, 산약, 쥐방울덩굴, 맥문동, 마늘, 차나무, 제비꽃, 조릿대, 조뱅이, 장구채, 닭의 장풀, 이질풀, 달래, 음나무, 끈끈이주걱, 은행나무, 꽈리, 인동덩굴, 깽깽이풀, 개미취, 개암풀, 감국, 용담, 가락풀나무, 오이풀, 질경이, 목향, 방풍, 측백나무, 백선, 한련초, 비파나무, 할미꽃, 산초나무, 현삼, 소태나무, 환삼덩굴, 협죽도, 형개, 홀아비꽃대 및 담쟁이덩굴로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물추출물; 및 레몬그라스(Lemongrass), 라벤더(Lavender), 스타아니스(Star anise), 만다린(Mandarin), 타임(Thyme), 잣나무, 오레가노(Oregano), 로즈제라늄(Rosegeranium), 티트리(Tea tree), 클로브리프(Clove leaf), 캐놀라(Canola), 피마자(Castor) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물성 오일을 유효성분으로 포함하는 조성물을 사용하는 경우, 미생물 배양에 소요되는 시간을 절감할 수 있고, 우수한 병해 방제효과를 나타낸다는 것을 규명함으로써 본 발명을 완성하게 되었다.
Thus, the inventors of the present invention (Portulaca oleracea), Echinacea (Echinacea), squirrel, Mangosteen, turmeric, boredom, mugwort, skewer, horse chestnut, barberry, tangerine, citrus fruit, unripe tree, yellow white, pomegranate, turmeric , Pine, rhubarb, celandine, hotcress, horseradish, golden, pore, bark grass, potion, rattan, ganmundong, garlic, tea tree, violet, stalk, zodiac, jangguchae, chicken's grass, cranesbill, soothing, rum, sticky spatula Ginkgo, Ginkgo, Honeysuckle, Daffodil, Anticidal, Hazelnut, Immaculate, Gentian, Rhododendron, Cucumber, Plantain, Cedar, Windproof, Cypress, Ringworm, Nasturtium, Loquat, Pasqueflower, Japanese Herb, Hyunsam, Plant extracts obtained by extracting any one or more selected from the group consisting of bovine tree, round ginseng vine, oleander, hyungae, widower and ivy; And Lemongrass, Lavender, Star anise, Mandarin, Thyme, Pine, Oregano, Roseegeranium, Tea tree, Claw When using a composition comprising a vegetable oil obtained by extracting from any one or more selected from the group consisting of Clove leaf, Canola, Castor and Nim as an active ingredient, microorganism culture is required The present invention has been completed by clarifying that it is possible to reduce the time required and to exhibit an excellent pest control effect.

본 발명의 일 양태에 따르면, 마치현(Portulaca oleracea), 에키나시아(Echinacea), 소리쟁이, 망고스틴(Mangosteen), 강황, 지치, 쑥, 도꼬마리, 우슬근, 황련, 감귤, 자소, 무환자나무, 황백, 석류, 울금, 소나무, 대황, 애기똥풀, 매운냉이, 민족두리풀, 황금, 자리공, 마디풀, 산약, 쥐방울덩굴, 맥문동, 마늘, 차나무, 제비꽃, 조릿대, 조뱅이, 장구채, 닭의 장풀, 이질풀, 달래, 음나무, 끈끈이주걱, 은행나무, 꽈리, 인동덩굴, 깽깽이풀, 개미취, 개암풀, 감국, 용담, 가락풀나무, 오이풀, 질경이, 목향, 방풍, 측백나무, 백선, 한련초, 비파나무, 할미꽃, 산초나무, 현삼, 소태나무, 환삼덩굴, 협죽도, 형개, 홀아비꽃대 및 담쟁이덩굴로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물추출물; 및 레몬그라스(Lemongrass), 라벤더(Lavender), 스타아니스(Star anise), 만다린(Mandarin), 타임(Thyme), 잣나무, 오레가노(Oregano), 로즈제라늄(Rosegeranium), 티트리(Tea tree), 클로브리프(Clove leaf), 캐놀라(Canola), 피마자(Castor) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물성 오일을 유효성분으로 포함하는 잔디 병해 방제용 조성물을 제공한다.According to an aspect of the present invention, Portulaca oleracea, Echinacea, Mandarin, Mangosteen, Turmeric, Chichi, Mugwort, Beetle, Hyssopmus, Rhododendron, Citrus fruit, Jasmine, Agarberry, Yellow white, Pomegranate, turmeric, pine, rhubarb, celandine, hotcress, horseshoe grass, golden, periwinkle, bark grass, potion, rattan, mackmundong, garlic, tea tree, violet, stalk, zinnia, jangguchae, chicken's grass, larvae, soothe Sticky spatula, ginkgo biloba, cherry tree, honeysuckle, decoction, anthill, hazelnut, country of origin, gentian, ivy, cucumber grass, plantain, cedar, windproof, cypress, ringworm, nasturtium, loquat, pasqueflower, vine Plant extracts obtained by extracting from any one or more selected from the group consisting of hyeonsam, bovine, hwansam vine, oleander, hyungae, widower and ivy; And Lemongrass, Lavender, Star anise, Mandarin, Thyme, Pine, Oregano, Roseegeranium, Tea tree, Claw Provided is a grass disease control composition comprising vegetable oil obtained by extracting from at least one selected from the group consisting of Clove leaf, Canola, Canola, Castor and Nim as an active ingredient.

본 발명의 식물추출물 또는 식물성 오일을 추출하는 경우 당업계에 공지된 통상적인 방법에 따라, 즉, 통상적인 온도, 압력의 조건 하에서 통상적인 용매를 사용하여 분리할 수 있다. 본 발명의 식물추출물 또는 식물성 오일을 추출하기 위한 추출 용매로는 추출공정에서 일반적으로 사용할 수 있는 용매를 사용할 수 있으며, 바람직하게는, 물, 탄소수 1-6 개의 무수 또는 함수 알코올, 헥산(hexane), 아세톤(acetone), 에틸아세테이트(Ethyl acetate), 부틸아세테이트(Butyl acetate), 1,3-부틸렌 글리콜(1,3-Butylene glycol) 또는 이들의 조합을 사용할 수 있다. 본 발명의 바람직한 구현예에 의하면, 본 발명의 추출물은 물, 탄소수 1-6 개의 무수 또는 함수 알코올, 헥산(hexane) 또는 이들의 조합을 용매로 하여 추출한 분획 추출물이며, 보다 바람직하게는 물, 메탄올, 부탄올, 헥산(hexane) 또는 이들의 조합의 용매 분획추출물이다.When extracting the plant extract or vegetable oil of the present invention can be separated according to conventional methods known in the art, that is, using a conventional solvent under the conditions of conventional temperature, pressure. As an extraction solvent for extracting the plant extract or vegetable oil of the present invention, a solvent that can be generally used in the extraction process may be used. Preferably, water, anhydrous or hydrous alcohol having 1 to 6 carbon atoms, and hexane Acetone (acetone), ethyl acetate (Ethyl acetate), butyl acetate (Butyl acetate), 1,3-butylene glycol (1,3-Butylene glycol) or a combination thereof may be used. According to a preferred embodiment of the present invention, the extract of the present invention is a fraction extract extracted using water, anhydrous or hydrous alcohol having 1 to 6 carbon atoms, hexane (hexane) or a combination thereof as a solvent, more preferably water, methanol , Solvent fraction extract of butanol, hexane or a combination thereof.

본 발명의 바람직한 구현예에 따르면, 상기 식물추출물은 마치현(Portulaca oleracea), 강황, 지치, 황련, 황백, 황금, 에키나시아(Echinacea), 망고스틴(Mangosteen), 우슬근, 자소, 무환자나무, 애기똥풀 및 민족두리풀로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출한 것이며, 식물성 오일은 레몬그라스(Lemongrass), 스타아니스(Star anise), 티트리(Tea tree), 만다린(Mandarin), 오레가노(Oregano) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 혼합물일 수 있다.According to a preferred embodiment of the present invention, the plant extract is Portulaca oleracea, turmeric, boredom, rye, yellow white, golden, Echinacea, Mangosteen, hyssop, jasper, unripe tree, celandine and Extracted from any one or more selected from the group consisting of Korean scallops, the vegetable oils are lemongrass, star anise, tea tree, mandarin, oregano and neem It may be a mixture obtained by extracting from any one or more selected from the group consisting of).

본 발명의 바람직한 구현예에 따르면, 본 발명의 조성물은 분산제, 산화방지제 및 제제화용 첨가제 중 어느 하나 이상을 더 포함할 수 있다.According to a preferred embodiment of the present invention, the composition of the present invention may further comprise any one or more of a dispersant, an antioxidant and a formulation additive.

본 발명에서 사용되는 용어 '분산제'는 계면활성 물질로서 혼합된 물질의 경계면에 흡착하여 경계면의 자유 에너지를 작게 하여 물질의 혼합상태를 용이하게 할 수 있는 물질을 말하며, 구체적으로는 비이온계면활성제가 사용될 수 있다. The term 'dispersant' used in the present invention refers to a material that can be adsorbed on the interface of the mixed material as a surfactant to reduce the free energy of the interface to facilitate the mixing state of the material, and specifically, a nonionic surfactant. Can be used.

비이온계면활성제란 물에 이온화되지 않고 용해되는 계면활성제를 말하며, 보다 구체적으로는 폴리옥시에틸렌 소르비탄 모노올레이트(Polyoxyethylene sorbitan monooleate), 폴리옥시에틸렌 트리데실 에테르(Polyoxyethylene tridecyl ether), 디메틸설폭사이드(Dimethylsulfoxide), 올리브리퀴드(Olive liquid), 폴리옥시에틸렌 글리세린 모노올레이트(Polyoxyethylene glycerin monooleate) 및 소르비탄 모노스테레이트(Sorbitan monostearate)로 이루어진 군에서 선택된 어느 하나 또는 둘 이상의 혼합물이 사용될 수 있으나 이에 한정되는 것은 아니다.Nonionic surfactants refer to surfactants that are not ionized and dissolved in water, and more specifically, polyoxyethylene sorbitan monooleate, polyoxyethylene tridecyl ether, and dimethyl sulfoxide. (Dimethylsulfoxide, Olive liquid, Polyoxyethylene glycerin monooleate and Sorbitan monostearate may be used, but is not limited thereto. It doesn't happen.

본 발명에서 사용되는 용어 '산화방지제'는 고분자물질, 석유제품, 유지류 또는 비누 등에 생기기 쉬운 산소의 작용에 의한 자동 산화를 방지하기 위해 첨가하는 물질을 의미하며, 구체적으로는 부틸화 하이드록시아니솔(Butylated hydroxy anisole), 프로필 갈레이트(Propyl gallete), 디부틸 하이드록시톨루엔(Dibutyl hydroxy toluene), 에르소르빈산(Erythorbic acid), 티셔리 부틸하이드로퀴논(tert-Butyl hydroquinone), 옥시스테아린(Oxystearin) 및 에틸렌디아민사초산이나트륨(Disodium Ethylenediaminetetraacetate)으로 이루어진 군에서 선택된 어느 하나 이상일 수 있으나 이에 한정되는 것은 아니다.As used herein, the term 'antioxidant' refers to a substance added to prevent automatic oxidation due to the action of oxygen, which is likely to occur in polymer materials, petroleum products, oils or soaps, and specifically, butylated hydroxyanisole. (Butylated hydroxy anisole), Propyl gallete, Dibutyl hydroxy toluene, Erythorbic acid, Tishary butyl hydroquinone, Oxystearin and It may be any one or more selected from the group consisting of disodium ethylenediamine tetraacetate (Disodium Ethylenediaminetetraacetate), but is not limited thereto.

본 발명에서 사용되는 용어 '제제화용 첨가제' 는 조성물의 안정화 정도를 높여 일정한 제형의 형태를 확보되도록 하는 물질을 의미하며, 구체적으로는 폴리옥시에틸렌 라우릴 에테르(Polyoxyethylene lauryl ether), 폴리옥시에틸렌 모노올레이트(Polyoxyethylene monooleate), 폴리옥시에틸렌 도데실 모노 에테르(Polyoxyethylene dodecyl mono ether), 폴리옥시에틸렌 폴리옥시프로필렌 알킬 에테르(Polyoxyethylene Polyoxypropylene Alkyl Ether), 폴리옥시에틸렌 캐스터 에테르(Polyoxyethylene Castor ether) 및 폴리옥시에틸렌 1,1,3,3-테트라메틸부틸 페닐 에테르(Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether)로 이루어진 군에서 선택된 어느 하나 이상일 수 있으나 이에 한정되는 것은 아니다.The term 'formulation additive' used in the present invention Refers to a substance that increases the degree of stabilization of the composition to ensure a certain form of the formulation, and specifically, polyoxyethylene lauryl ether, polyoxyethylene monooleate, polyoxyethylene dode Polyoxyethylene dodecyl mono ether, Polyoxyethylene Polyoxypropylene Alkyl Ether, Polyoxyethylene Castor ether and Polyoxyethylene 1,1,3,3-tetramethylbutyl It may be one or more selected from the group consisting of phenyl ether (Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether), but is not limited thereto.

본 발명의 바람직한 구현예에 따르면, 본 발명의 식물추출물 및 식물성 오일의 중량 합계는 조성물의 총 중량 대비 10 - 70 중량%이며, 보다 바람직하게는 40 - 60 중량%, 가장 바람직하게는 45 - 55 중량%이다. According to a preferred embodiment of the present invention, the sum of the weights of the plant extracts and vegetable oils of the present invention is 10-70% by weight, more preferably 40-60% by weight, most preferably 45-55, relative to the total weight of the composition. Weight percent.

본 발명의 바람직한 구현예에 따르면, 본 발명의 분산제와 산화방지제는 조성물의 총 중량 대비 1 - 20 중량%이며, 보다 바람직하게는 5 - 15 중량%, 가장 바람직하게는 5 - 10 중량%이다.According to a preferred embodiment of the invention, the dispersant and antioxidant of the invention are 1-20% by weight, more preferably 5-15% by weight, most preferably 5-10% by weight, relative to the total weight of the composition.

본 발명의 바람직한 구현예에 따르면, 본 발명의 제제화용 첨가제는 조성물의 총 중량 대비 20 - 60 중량%이며, 보다 바람직하게는 30 - 50 중량%, 가장 바람직하게는 35 - 45 중량%이다.According to a preferred embodiment of the present invention, the formulation additive of the present invention is 20 to 60% by weight, more preferably 30 to 50% by weight, most preferably 35 to 45% by weight relative to the total weight of the composition.

본 발명에서 사용되는 용어 '잔디 병해'는 기주식물, 병원균 및 발병환경의 영향으로 잔디 생장에 피해를 주는 것을 의미하며, 갈색퍼짐병(Large patch), 춘고병, 달라스팟(Dollar spot), 옐로우 패취(Yellow patch), 탄저병(Anthracnose), 잔디도열병(Gray leaf spot), 입고병, 갈색마름병(brown patch), 피시움마름병(Pythium blight), 녹병(Rust), 페어리링(fairy-ring), 설부갈색소립균핵병(Typhula blight) 및 엽고병(Leaf blight) 등이 있으며, 구체적으로는 갈색퍼짐병(Large patch) 또는 갈색마름병(brown patch) 일 수 있으나 이에 한정되는 것은 아니다.
The term 'grass disease' used in the present invention means damage to grass growth under the influence of host plants, pathogens and the pathogenic environment, brown patch, spring disease, Dollar spot, yellow Yellow patch, Anthracnose, Gray leaf spot, Wear bottle, Brown patch, Pythium blight, Rust, Fairy-ring, Sulfur brown microbial disease (Typhula blight) and leaf blight (Leaf blight) and the like, specifically, it may be a brown spread disease (Large patch) or brown blight (brown patch), but is not limited thereto.

본 발명의 잔디 병해 방제용 조성물은 미생물을 이용하는 종래의 잔디 병해 방제 방법보다 병원균을 억제하기 위해 이용되는 미생물 배양에 소요되는 시간을 절감하고 우수한 방제효과를 지니므로 실효성이 높다.The grass disease control composition of the present invention is highly effective because it reduces the time required for culturing microorganisms used to suppress pathogens and has an excellent control effect than conventional grass disease control methods using microorganisms.

또한, 살균 및 항균효과가 우수한 식물추출물 및 식물성 오일을 제공함으로서 내성 유발이 없고, 인체에 무해한 조성물을 제공할 수 있다.
In addition, by providing a plant extract and vegetable oil excellent in the bactericidal and antibacterial effect, there is no induction of resistance, it can provide a composition harmless to the human body.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

<< 실시예Example 1> 64종의 식물체로부터  1> From 64 plant species 조추출물Crude extract 제조 Produce

여러 문헌을 통하여 각종 식물병원균에 대해 살균효과가 예상되는 표1의 식물체 64종을 선별한 후, 이를 각각 100g씩 믹서기에서 곱게 마쇄하여 유리병에 넣은 다음 메탄올 1000ml를 혼합하여 약 60℃에서 24시간 방치하였다. 24시간 후 여과하고 추출용액을 회전진공감압농축기(EYELA N-1000, 일본)로 농축하였다. 상기 농축물을 다시 메탄올에 혼합하는 과정을 거쳐 메탄올 조추출물을 제조하였다.
Through various literatures, 64 kinds of plants of Table 1, which are expected to have bactericidal effects against various phytopathogens, were selected, and then 100 g of these were finely ground in a blender, put into glass bottles, and 1000 ml of methanol was mixed for 24 hours at about 60 hours. It was left. After 24 hours, the mixture was filtered and the extract solution was concentrated using a rotary vacuum concentrator (EYELA N-1000, Japan). The concentrate was further mixed with methanol to prepare a crude methanol extract.

식물명Botanical name 학명Scientific name 식물명Botanical name 학명Scientific name 마치현March Portulaca oleraceaPortulaca oleracea 에키나시아 Echinacea Echinacea purpureaEchinacea purpurea 소리쟁이Sounder Rumex crispusRumex crispus 망고스틴Mangosteen Garcinia mangostanaGarcinia mangostana 강황curcuma Curcuma aromaticaCurcuma aromatica 지치Tired Lithospermum erythrorhizonLithospermum erythrorhizon Mugwort Artemisia princepsArtemisia princeps 도꼬마리Macaw Xanthium strumariumXanthium strumarium 우슬근Right muscle Achyranthes japonicaAchyranthes japonica 황련goldthread Coptis japonicaCoptis japonica 감귤Citrus Citrus unshiuCitrus unshiu 자소Jaso Perilla frutescensPerilla frutescens 무환자나무Tree Sapindus mukorossiSapindus mukorossi 황백Yellowish white Phellodendron amurensePhellodendron amurense 석류Pomegranate Punica granatumPunica granatum 울금Turmeric Curcuma longaCurcuma longa 소나무Pine tree Pinus densifloraPinus densiflora 대황 rhubarb Rheum undulatumRheum undulatum 애기똥풀celandine Chelidonium majusChelidonium majus 매운냉이Spicy Wasabi Wasabia japonicaWasabia japonica 민족두리풀Ethnic scroll Asiasarum heterotropoidesAsiasarum heterotropoides 황금Gold Scutellaria baicalensisScutellaria baicalensis 자리공Seat Phytolacca esculentaPhytolacca esculenta 마디풀knotgrass Polygonum avicularePolygonum aviculare 산약Medicine Dioscorea batatasDioscorea batatas 쥐방울덩굴Rat vine Aristolochia contortaAristolochia contorta 맥문동McMundong Liriope platyphylla Liriope platyphylla 마늘garlic Allium sativumAllium sativum 차나무tea Melaleuca alternifoliaMelaleuca alternifolia 제비꽃violet Viola mandshuricaViola mandshurica 조릿대Scoop Sasamorpha purpurascensSasamorpha purpurascens 조뱅이Jovan Cephalonopsis segetusCephalonopsis segetus 장구채Jangguchae Melandrium firmumMelandrium firmum 닭의 장풀Fowl of chicken Commelina communisCommelina communis 이질풀cranesbill Geranium nepalenseGeranium nepalense 달래Soothe Allium grayiAllium grayi 음나무Yin Kalopanax pictusKalopanax pictus 끈끈이주걱Sticky spatula Drosera rotundifolia Drosera rotundifolia 은행나무Ginkgo Ginkgo bilobaGinkgo biloba 꽈리ground cherry Physalis franchetiiPhysalis franchetii 인동덩굴Honeysuckle Lonicera japonicaLonicera japonica 깽깽이풀Poker Plagiorhegma dubium Plagiorhegma dubium 개미취Michaelmas daisy Aster tataricusAster tataricus 개암풀Hazel Psoralea corylifolia Psoralea corylifolia 감국Country Chrysanthemum morifoliumChrysanthemum morifolium 용담Gentian Gentiana scabraGentiana scabra 가락풀나무Buckthorn Caulophyllum thalictroicdesCaulophyllum thalictroicdes 오이풀Cucumber Sanguisorba officinalisSanguisorba officinalis 질경이Plantain Plantago asiaticaPlantago asiatica 목향elecampane Inula helenium Inula helenium 방풍Wind wind Saposhnikovia divaricataSaposhnikovia divaricata 측백나무Cypress Thuja orientalisThuja orientalis 백선ringworm Dictamnus dasycarpusDictamnus dasycarpus 한련초Nasturtium Eclipta prostataEclipta prostata 비파나무Loquat Eryobotrya japonica Eryobotrya japonica 할미꽃pasqueflower Pulsatilla koreanaPulsatilla koreana 산초나무Japanese tree Zanthoxylum piperitumZanthoxylum piperitum 현삼figwort Scrophularia oldhamiScrophularia oldhami 소태나무Pine tree Picrasma ailanthoides Picrasma ailanthoides 환삼덩굴Round ginseng Humulus japonicusHumulus japonicus 협죽도Oekoji Nerium oleanderNerium oleander 형개Mold Schizonepeta tenuifoliaSchizonepeta tenuifolia 홀아비꽃대Widower Chloranthus japonicusChloranthus japonicus 담쟁이덩굴Ivy Parthenocissus tricuspidataParthenocissus tricuspidata

<< 실시예Example 2>  2> 조추출물의Crude extract 갈색퍼짐병Brown spread disease  And 갈색마름병Brown blight 병원균에 대한 항진균 활성평가 Antifungal Activity Assessment of Pathogens

상기에서 준비한 64종 조추출물의 항진균 활성평가를 위하여 갈색퍼짐병 병원균(Rhizoctonia solani AG2-2) 및 갈색마름병 병원균(Rhizoctonia solani AG1-1)에 대한 항진균 활성을 평가하였다. 평가방법은 페이퍼 디스크(paper disc)방법에 준하여 각각의 조추출물을 500 ppm/disc, 1000 ppm/disc 농도로 점적하여 항진균 활성을 평가하고 결과를 하기 표 2에 나타내었다.
In order to evaluate the antifungal activity of 64 crude extracts prepared above, antifungal activity against brown spread disease pathogen (Rhizoctonia solani AG2-2) and brown blight pathogen (Rhizoctonia solani AG1-1) was evaluated. Evaluation method was to drop the crude extract in 500 ppm / disc, 1000 ppm / disc concentration according to the paper disc method (evaluation) antifungal activity and the results are shown in Table 2 below.

조추출물Crude extract
처리농도(Treatment concentration ( ppmppm )) 조추출물Crude extract
처리농도(Treatment concentration ( ppmppm ))
500500 10001000 500500 10001000 마치현March 2020 1717 에키나시아Echinacea 2121 1818 소리쟁이Sounder 2121 1818 망고스틴Mangosteen 2323 2020 강황curcuma 2121 1919 지치Tired 2121 1818 Mugwort 2121 1919 도꼬마리Macaw 2323 2020 우슬근Right muscle 2626 2121 황련goldthread 2222 1919 tangerine 2222 2020 자소Jaso 2323 1818 무환자나무Tree 2626 2222 황백Yellowish white 2222 1919 석류Pomegranate 2424 2121 울금Turmeric 2222 1919 소나무Pine tree 2222 2020 대황rhubarb 2121 1818 애기똥풀celandine 2525 2020 매운냉이Spicy Wasabi 2626 2121 민족두리풀Ethnic scroll 2525 2020 황금Gold 2222 1818 자리공Seat 2424 2121 마디풀knotgrass 2323 1919 산약Medicine 2222 2020 쥐방울덩굴Rat vine 2424 1919 맥문동McMundong 2525 2222 마늘garlic 2525 2020 차나무tea 2121 1919 제비꽃violet 2525 2020 조릿대Scoop 2525 2121 조뱅이Jovan 2424 1919 장구채Jangguchae 2525 2222 닭의 장풀Fowl of chicken 2323 1818 이질풀cranesbill 2525 2323 달래Soothe 2424 2121 음나무Yin 2525 2020 끈끈이주걱Sticky spatula 2424 2121 은행나무Ginkgo 2525 1919 꽈리ground cherry 2424 1919 인동덩굴Honeysuckle 2323 2020 깽깽이풀Poker 2525 2020 개미취Michaelmas daisy 2424 1919 개암풀Hazel 2626 2121 감국Country 2222 1919 용담Gentian 2525 1919 가락풀나무Buckthorn 2323 1919 오이풀Cucumber 2626 2121 질경이Plantain 2323 1818 목향elecampane 2626 2121 방풍Wind wind 2222 1818 측백나무Cypress 2222 1919 백선ringworm 2222 1818 한련초Nasturtium 2626 2121 비파나무Loquat 2424 1919 할미꽃pasqueflower 2626 2121 산초나무Japanese tree 2222 1919 현삼figwort 2525 2020 소태나무Pine tree 2222 1919 환삼덩굴Round ginseng 2626 2222 협죽도Oekoji 2525 1919 형개Mold 2525 2020 홀아비꽃대Widower 2626 2121 담쟁이덩굴Ivy 2222 1818 ControlControl 4040

표 2는 조추출물의 갈색퍼짐병 및 갈색마름병 병원균에 대한 항진균 활성평가를 나타낸 실험결과표이다.(생육직경; mm)
Table 2 is an experimental result table showing the antifungal activity of crude extracts against brown spread disease and brown blight pathogens (growth diameter; mm).

<< 실시예Example 3> 13종의 식물체로부터 식물성 오일 제조 3> Vegetable Oils from 13 Plants

여러 문헌을 통하여 각종 식물 병원균에 대해 살균효과가 알려져 있는 하기 표 3의 식물체로부터 수증기 증류법(Steam distillation)을 실시하여 식물성 오일을 제조하였다.
Vegetable oil was prepared by steam distillation (Steam distillation) from the plants of Table 3, which are known to have various bactericidal effects against various plant pathogens.

식물명Botanical name 학명Scientific name 라벤더(Lavender)Lavender Lavandula angustifoliaLavandula angustifolia 스타아니스(Star anise)Star anise Illicium verumIllicium verum 만다린(Mandarin)Mandarin Citrus reticulataCitrus reticulata 타임(Thyme)Thyme Thymus vulgarisThymus vulgaris 레몬그라스(Lemongrass)Lemongrass Cymbopogon citratusCymbopogon citratus 잣나무(Nut Pine)Nut Pine Pinus koraiensisPinus koraiensis 오레가노(Oregano)Oregano Origanum vulgareOriganum vulgare 로즈제라늄(Rosegeranium)Rose geranium Pelargonium rosiumPelargonium rosium 티트리(Tea tree)Tea tree Melaleuca alternifoliaMelaleuca alternifolia 클로브리프(Clove leaf)Clove leaf Eugenia aromaticumEugenia aromaticum 캐놀라(Canola)Canola Brassica napusBrassica napus 피마자(Castor)Castor Ricinus communisRicinus communis 님(Neem)Nim Melia azadirachtaMelia azadirachta

표 3은 식물성 오일을 제조하기 위한 13종의 식물체를 나타낸 표이다.
Table 3 is a table showing 13 plants for producing vegetable oils.

<< 실시예Example 4> 식물성 오일의  4> of vegetable oil 갈색퍼짐병Brown spread disease  And 갈색마름병Brown blight 병원균에 대한 항진균 활성평가 Antifungal Activity Assessment of Pathogens

상기에서 준비한 13종의 식물성 오일의 항진균 활성평가를 위하여 갈색퍼짐병 병원균(Rhizoctonia solani AG2-2) 및 갈색마름병 병원균(Rhizoctonia solani AG1-1)에 대한 항진균 활성을 평가하였다. 평가방법은 페이퍼 디스크(paper disc)방법에 준하여 각각의 조추출물을 500 ppm/disc, 1000 ppm/disc 농도로 점적하여 항진균 활성을 평가하고 결과를 하기 표 4에 나타내었다.
In order to evaluate the antifungal activity of the 13 vegetable oils prepared above, antifungal activity against brown spread disease pathogen (Rhizoctonia solani AG2-2) and brown blight pathogen (Rhizoctonia solani AG1-1) was evaluated. The evaluation method was to drop the crude extract at a concentration of 500 ppm / disc, 1000 ppm / disc in accordance with the paper disc method to evaluate the antifungal activity and the results are shown in Table 4 below.

식물성 Vegetable 오일명Oil name
처리농도(Treatment concentration ( ppmppm ))
500500 10001000 라벤더(Lavender)Lavender 3333 2525 스타아니스(Star anise)Star anise 2424 2222 만다린(Mandarin)Mandarin 3636 2525 타임(Thyme)Thyme 2424 2222 레몬그라스(Lemongrass)Lemongrass 2020 1616 잣나무(Nut Pine)Nut Pine 3434 2626 오레가노(Oregano)Oregano 3333 2626 로즈제라늄(Rosegeranium)Rose geranium 3232 2424 티트리(Tea tree)Tea tree 2323 2121 클로브리프(Clove leaf)Clove leaf 3333 2626 캐놀라(Canola)Canola 2222 1818 피마자(Castor)Castor 2323 1919 님(Neem)Nim 3232 2424 ControlControl 4040

표 4는 식물성 오일의 갈색퍼짐병 및 갈색마름병 병원균에 대한 항진균 활성평가를 나타낸 실험결과표이다.(생육직경; mm)
Table 4 is an experimental result table showing the antifungal activity of vegetable oil against brown spread disease and brown blight pathogens (growth diameter; mm).

<< 실시예Example 5> 2가지 유효성분 혼합에 따른 항진균 활성 평가 5> Evaluation of antifungal activity by mixing two active ingredients

상기에서 선발된 2가지 유효성분(마치현추출물, 레몬그라스오일)의 살균효과를 보다 효율적으로 증진시키기 위해 각각의 유효성분을 단독으로 사용하는 것과 2가지 유효성분을 동일한 비율로 혼합하여 사용하는 경우 갈색퍼짐병 및 갈색마름병 병원균에 대한 항진균 활성을 평가하였다.
In order to more effectively enhance the bactericidal effect of the two selected active ingredients (Machi Prefecture Extract, Lemongrass Oil), each of the active ingredients is used alone and the two active ingredients are mixed in the same ratio. Antifungal activity against spread and brown blight pathogens was evaluated.

유효성분Active ingredient
처리농도(Treatment concentration ( ppmppm ))
500500 10001000 마치현추출물March extract 2020 1717 레몬그라스오일Lemongrass Oil 2020 1616 마치현추출물 + 레몬그라스오일March Extract + Lemongrass Oil 1515 1010 ControlControl 4040

표 5는 유효성분 혼합에 따른 갈색퍼짐병 및 갈색마름병 병원균에 대한 항진균 활성평가를 나타낸 실험결과표이다.(생육직경; mm)
Table 5 is an experimental result table showing the evaluation of antifungal activity against the brown spread disease and brown blight pathogens according to the mixing of the active ingredient (growth diameter; mm)

<< 실시예Example 6> 효력증진제가 더 포함된 살균제 제조 6> Manufacture of fungicides containing more potentiators

상기 표 5에서 선발된 2가지 유효성분의 살균효과를 증진하기 위한 효력증진제로 분산제 및 산화방지제를 첨가하였다.Dispersants and antioxidants were added as potentiators to enhance the bactericidal effect of the two active ingredients selected in Table 5.

최적의 분산제를 선발하기 위해서 분산제 중에서 비이온계면활성제인 폴리옥시에틸렌 소르비탄 모노올레이트(Polyoxyethylene sorbitan monooleate), 폴리옥시에틸렌 트리데실 에테르(Polyoxyethylene tridecyl ether), 디메틸설폭사이드(Dimethylsulfoxide), 올리브리퀴드(Olive liquid), 폴리옥시에틸렌 글리세린 모노올레이트(Polyoxyethylene glycerin monooleate) 및 소르비탄 모노스테레이트(Sorbitan monostearate)를 이용하여 각각 식물추출물 및 식물성 오일이 골고루 분산될 수 있도록 실험을 수행하였다. 각각의 물질을 농도별로 희석하고, 혼합한 다음 현미경을 이용하여 분산정도를 비교한 결과 폴리옥시에틸렌 소르비탄 모노올레이트(Polyoxyethylene sorbitan monooleate) 8 % 이상에서 가장 양호한 것으로 나타났다. Among the dispersants, polyoxyethylene sorbitan monooleate, polyoxyethylene tridecyl ether, dimethylsulfoxide, olive liquid ( Olive liquid), polyoxyethylene glycerin monooleate and sorbitan monostearate (Sorbitan monostearate) using an experiment was carried out so that the plant extract and vegetable oil can be evenly dispersed, respectively. Dilutions of the individual substances by concentration, mixing and dispersing the results using a microscope showed the best results at 8% or more of polyoxyethylene sorbitan monooleate.

산화방지제는 화장품 및 식품용으로 사용되고 있는 여러 종류의 산화방지제를 이용하여 유효성분인 식물추출물과 식물성오일의 산화를 효율적으로 방지할 수있도록 하였다. 산화방지제는 부틸화 하이드록시아니솔(Butylated hydroxy anisole), 프로필 갈레이트(Propyl gallete), 디부틸 하이드록시톨루엔(Dibutyl hydroxy toluene), 에르소르빈산(Erythorbic acid), 티셔리 부틸하이드로퀴논(tert-Butyl hydroquinone), 옥시스테아린(Oxystearin) 및 에틸렌디아민사초산이나트륨(Disodium Ethylenediaminetetraacetate) 각각을 사용하여 최적의 분산제를 선발시험 하였다. 각각의 산화방지제를 농도별로 처리하여 유효성분과 혼합한 뒤 온도를 달리하여 경시적으로 보관하면서 항균효과의 변화를 비교하여 산화방지여부를 확인한 결과 부틸화 하이드록시아니솔(Butylated hydroxy anisole) 2 % 이상에서 산화방지효과가 우수한 것으로 나타났다.
Antioxidants were used to prevent oxidation of plant extracts and vegetable oils, which are effective ingredients, by using various kinds of antioxidants used for cosmetics and foods. Antioxidants include butylated hydroxy anisole, propyl gallete, dibutyl hydroxy toluene, erythorbic acid, and butyl butylhydroquinone (tert-Butyl) The optimal dispersant was selected using hydroquinone, Oxystearin and Disodium Ethylenediaminetetraacetate. Each antioxidant was treated by concentration and mixed with the active ingredient, and then stored at different temperatures and compared with changes in the antimicrobial effect to confirm the anti-oxidation result. Butylated hydroxy anisole was not less than 2%. The antioxidant effect was found to be excellent at.

<< 실시예Example 7>  7> 제제화용Formulation 첨가제 및 안정제가 더 포함된 살균제 제조 Preparation of fungicides containing additives and stabilizers

본 발명의 2가지 유효성분, 분산제 및 산화방지제의 제제화를 위해서는 제제화용 첨가제가 요구된다. 따라서, 실시예 6에서 선별한 분산제 및 산화방지제와 2가지 유효성분의 혼합물에 제제화용 첨가제로서 다양한 비이온계면활성제를 농도별로 혼합하여 물성변화를 관찰하고 제제화용 첨가제를 선별하였다. 그 결과, 제제화용 첨가제로서 폴리옥시에틸렌 라우릴 에테르(Polyoxyethylene lauryl ether) 15 %, 폴리옥시에틸렌 모노올레이트(Polyoxyethylene monooleate) 5 % , 폴리옥시에틸렌 도데실 모노 에테르(Polyoxyethylene dodecyl mono ether) 5 %, 폴리옥시에틸렌 캐스터 에테르(Polyoxyethylene Castor ether) 5 % 및 폴리옥시에틸렌 1,1,3,3-테트라메틸부틸 페닐 에테르(Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether) 10 % 이상 혼합하였을 경우 유효성분의 침전, 층분리 등이 없는 것으로 나타났다.
Formulation additives are required for the formulation of the two active ingredients, dispersants and antioxidants of the present invention. Therefore, in the mixture of the dispersant and antioxidant selected from Example 6 and the two active ingredients as a formulation additive, various nonionic surfactants were mixed for each concentration to observe the change in physical properties and the formulation additive was selected. As a result, 15% polyoxyethylene lauryl ether, 5% polyoxyethylene monooleate, 5% polyoxyethylene dodecyl mono ether, Effective when 5% of polyoxyethylene castor ether and 10% of polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether are mixed There was no precipitation of the components, no separation of the layers, etc.

<< 실시예Example 8> 최종배합에 따른 살균제 제조 8> Preparation of disinfectant according to the final blend

상기에서 선발된 2가지 유효성분, 분산제, 산화방지제 및 제제화용 첨가제를 이용하여 최종배합에 따른 살균제를 제조하였다. 유효성분인 마치현추출물은 25 %, 레몬그라스오일은 25 %를 혼합하였다. 분산제는 폴리옥시에틸렌 소르비탄 모노올레이트(Polyoxyethylene sorbitan monooleate) 8 %, 산화방지제는 부틸화 하이드록시아니솔(Butylated hydroxy anisole) 2 % 를 혼합하였다. 제제화용 첨가제는 폴리옥시에틸렌 라우릴 에테르(Polyoxyethylene lauryl ether) 15 %, 폴리옥시에틸렌 모노올레이트(Polyoxyethylene monooleate) 5 % , 폴리옥시에틸렌 도데실 모노 에테르(Polyoxyethylene dodecyl mono ether) 5 %, 폴리옥시에틸렌 캐스터 에테르(Polyoxyethylene Castor ether) 5 % 및 폴리옥시에틸렌 1,1,3,3-테트라메틸부틸 페닐 에테르(Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether) 10 % 를 혼합하여 살균제를 제조하였다.
Using the two active ingredients, dispersants, antioxidants and formulation additives selected from the above to prepare a fungicide according to the final formulation. March extract, 25% of lemongrass oil was mixed as an active ingredient. The dispersant was mixed with 8% polyoxyethylene sorbitan monooleate, and the antioxidant was mixed with 2% butylated hydroxy anisole. Formulation additives include: 15% polyoxyethylene lauryl ether, 5% polyoxyethylene monooleate, 5% polyoxyethylene dodecyl mono ether, polyoxyethylene A fungicide was prepared by mixing 5% polyoxyethylene castor ether and 10% polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether.

본 발명의 살균제의 포장에서의 방제효과 시험Control effect test in packaging of fungicide of the present invention

<< 실험예Experimental Example 1> 과채류 흰가루병에 대한 방제효과 시험 1> Control effect test for fruit powder powdery mildew

실시예 8에서 제조된 조성물을 갈색퍼짐병 및 갈색마름병이 발병한 골프장 잔디에서 방제효과를 시험하였다. 포장에서의 방제효과 검정은 500배로 희석하여 발병초 7일 간격으로 3회 경엽처리 하였으며, 대조약제로는 토로스액상수화제(tolcolfos-methyl)와 비교하였다. 포장관리는 골프장 관리기준에 준하였고, 시험구는 난괴법을 3회 반복하여 실시하였으며, 조사방법은 최종약제 처리 후 발병도 및 이병율을 조사하였다.
The composition prepared in Example 8 was tested for control effect in the grass turf caused by brown spread disease and brown blight disease. The control effect on the packaging was diluted 500-fold and treated with three leaves at intervals of 7 days at the beginning of the development. Pavement management was based on the golf course management criteria, test zones were repeated three times the egg mass method, the investigation method was investigated the incidence and morbidity rate after the final drug treatment.

시험약제Test drug
발병도(%)Incidence (%) 방제가(%)Control (%)
1반복1 repetition 2반복2 repetitions 3반복3 repetitions 평균Average 실시예8(500배)Example 8 (500 times) 8.78.7 7.97.9 5.35.3 7.37.3 84.384.3 토로스 수화제(500배)Toros Hydration (500 times) 7.67.6 4.04.0 3.93.9 5.25.2 8989 무처리No treatment 41.041.0 45.345.3 53.153.1 46.546.5 --

표 6은 갈색퍼짐병에 대한 방제효과를 나타낸 실험결과표이다.
Table 6 is an experimental result table showing the control effect for brown spread disease.

시험약제Test drug
발병도(%)Incidence (%) 방제가(%)Control (%)
1반복1 repetition 2반복2 repetitions 3반복3 repetitions 평균Average 실시예8(500배)Example 8 (500 times) 10.010.0 8.28.2 5.55.5 7.97.9 81.281.2 토로스 수화제(500배)Toros Hydration (500 times) 4.14.1 3.53.5 2.62.6 3.43.4 91.991.9 무처리No treatment 31.031.0 42.342.3 53.153.1 42.142.1 --

표 7은 갈색마름병에 대한 방제효과를 나타낸 실험결과표이다.
Table 7 is an experimental result table showing the control effect against brown blight.

상기 표 6 내지 표 7에서 보는 바와 같이 실시예 8의 500배에서 갈색퍼짐병에 대한 방제효과는 84.3 %의 방제가를 나타내고, 갈색마름병에 대한 방제효과는 81.2 %를 나타내고 있다. 일반적인 미생물농약이나 기타 천연물제제의 방제효과가 70 % 내외인 점을 감안할 때 상대적으로 높은 방제효과를 보였다. As shown in Table 6 to Table 7, the control effect against brown spread disease was 84.3%, and the control effect against brown blight disease was 81.2% at 500 times of Example 8. Considering that the control effect of general microbial pesticides and other natural products is about 70%, the control effect is relatively high.

Claims (10)

마치현(Portulaca oleracea), 에키나시아(Echinacea), 소리쟁이, 망고스틴(Mangosteen), 강황, 지치, 쑥, 도꼬마리, 우슬근, 황련, 감귤, 자소, 무환자나무, 황백, 석류, 울금, 소나무, 대황, 애기똥풀, 매운냉이, 민족두리풀, 황금, 자리공, 마디풀, 산약, 쥐방울덩굴, 맥문동, 마늘, 차나무, 제비꽃, 조릿대, 조뱅이, 장구채, 닭의 장풀, 이질풀, 달래, 음나무, 끈끈이주걱, 은행나무, 꽈리, 인동덩굴, 깽깽이풀, 개미취, 개암풀, 감국, 용담, 가락풀나무, 오이풀, 질경이, 목향, 방풍, 측백나무, 백선, 한련초, 비파나무, 할미꽃, 산초나무, 현삼, 소태나무, 환삼덩굴, 협죽도, 형개, 홀아비꽃대 및 담쟁이덩굴로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물추출물; 및 레몬그라스(Lemongrass), 라벤더(Lavender), 스타아니스(Star anise), 만다린(Mandarin), 타임(Thyme), 잣나무, 오레가노(Oregano), 로즈제라늄(Rosegeranium), 티트리(Tea tree), 클로브리프(Clove leaf), 캐놀라(Canola), 피마자(Castor) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출하여 얻어진 식물성 오일을 유효성분으로 포함하는 잔디 병해 방제용 조성물.
Portulaca oleracea, Echinacea, Mandarin, Mangosteen, Turmeric, Chichi, Wormwood, Beetle, Hyssop, Rhododendron, Citrus Fruit, Cherry, Unripe Tree, Yellow White, Pomegranate, Turmeric, Pine, Rhubarb, Celandine, Spicy horseradish, Pelicans, Golden, Periwinkle, Mardiflora, Pomegranate, Ratberry, Macmundong, Garlic, Tea tree, Violet, Sprout, Zinnia, Jangchae, Roe of chicken, Della grass, Soothe, Yin, Sticky spatula, Ginkgo biloba Honeysuckle, honeysuckle, antler, hazel, sensation, gentian, gingko, cucumber, plantain, cedar, windproof, cypress, ringworm, nasturtium, loquat, pasqueflower, japanese vine, hyunsam, pineapple, fern Plant extracts obtained by extracting from any one or more selected from the group consisting of oleanders, hyungae, widower and ivy; And Lemongrass, Lavender, Star anise, Mandarin, Thyme, Pine, Oregano, Roseegeranium, Tea tree, Claw A composition for controlling grass diseases comprising vegetable oil obtained by extracting from any one or more selected from the group consisting of Clove leaf, Canola, Canola, Castor and Nim as an active ingredient.
제 1 항에 있어서,
상기 식물추출물은 마치현(Portulaca oleracea), 강황, 지치, 황련, 황백, 황금, 에키나시아(Echinacea), 망고스틴(Mangosteen), 우슬근, 자소, 무환자나무, 애기똥풀 및 민족두리풀로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출한 것이며, 식물성 오일은 레몬그라스(Lemongrass), 스타아니스(Star anise), 티트리(Tea tree), 만다린(Mandarin), 오레가노(Oregano) 및 님(Neem)으로 이루어진 군에서 선택된 어느 하나 이상으로부터 추출한 것을 특징으로 하는 조성물.
The method of claim 1,
The plant extract is any one selected from the group consisting of Portulaca oleracea, turmeric, chichi, rhododendron, yellow white, golden, Echinacea, mangosteen, hyssopmus, japonica, amorous tree, celandine, Extracted from the above, the vegetable oil is at least one selected from the group consisting of Lemongrass, Star anise, Tea tree, Mandarin, Oregano and Neem A composition, characterized in that it is extracted from.
제 1 항에 있어서,
분산제, 산화방지제 및 제제화용 첨가제 중 어느 하나 이상을 더 포함하는 것을 특징으로 하는 조성물.
The method of claim 1,
A composition comprising any one or more of dispersants, antioxidants and formulation additives.
제 3 항에 있어서,
상기 분산제는 비이온계면활성제인 것을 특징으로 하는 조성물.
The method of claim 3, wherein
The dispersant is a composition, characterized in that the nonionic surfactant.
제 4 항에 있어서,
상기 비이온계면활성제는 폴리옥시에틸렌 소르비탄 모노올레이트(Polyoxyethylene sorbitan monooleate), 폴리옥시에틸렌 트리데실 에테르(Polyoxyethylene tridecyl ether), 디메틸설폭사이드(Dimethylsulfoxide), 올리브리퀴드(Olive liquid), 폴리옥시에틸렌 글리세린 모노올레이트(Polyoxyethylene glycerin monooleate) 및 소르비탄 모노스테레이트(Sorbitan monostearate)로 이루어진 군에서 선택된 어느 하나 이상인 것을 특징으로 하는 조성물.
The method of claim 4, wherein
The nonionic surfactant may be polyoxyethylene sorbitan monooleate, polyoxyethylene tridecyl ether, dimethylsulfoxide, olive liquid, polyoxyethylene glycerine Monooleate (Polyoxyethylene glycerin monooleate) and sorbitan (Sorbitan monostearate) A composition, characterized in that any one or more selected from the group consisting of.
제 3 항에 있어서,
상기 산화방지제는 부틸화 하이드록시아니솔(Butylated hydroxy anisole), 프로필 갈레이트(Propyl gallete), 디부틸 하이드록시톨루엔(Dibutyl hydroxy toluene), 에르소르빈산(Erythorbic acid), 티셔리 부틸하이드로퀴논(tert-Butyl hydroquinone), 옥시스테아린(Oxystearin) 및 에틸렌디아민사초산이나트륨(Disodium Ethylenediaminetetraacetate)으로 이루어진 군에서 선택된 어느 하나 이상인 것을 특징으로 하는 조성물.
The method of claim 3, wherein
The antioxidants are butylated hydroxy anisole, propyl gallete, dibutyl hydroxy toluene, erythorbic acid, and butyl butylhydroquinone (tert- Butyl hydroquinone), Oxystearin (Oxystearin) and Ethylenediamine disodium acetate (Disodium Ethylenediaminetetraacetate) A composition, characterized in that any one or more selected from the group consisting of.
제 3 항에 있어서,
상기 제제화용 첨가제는 폴리옥시에틸렌 라우릴 에테르(Polyoxyethylene lauryl ether), 폴리옥시에틸렌 모노올레이트(Polyoxyethylene monooleate), 폴리옥시에틸렌 도데실 모노 에테르(Polyoxyethylene dodecyl mono ether), 폴리옥시에틸렌 폴리옥시프로필렌 알킬 에테르(Polyoxyethylene Polyoxypropylene Alkyl Ether), 폴리옥시에틸렌 캐스터 에테르(Polyoxyethylene Castor ether) 및 폴리옥시에틸렌 1,1,3,3-테트라메틸부틸 페닐 에테르(Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether)로 이루어진 군에서 선택된 어느 하나 이상인 것을 특징으로 하는 조성물.
The method of claim 3, wherein
The formulation additives include polyoxyethylene lauryl ether, polyoxyethylene monooleate, polyoxyethylene dodecyl mono ether, polyoxyethylene polyoxypropylene alkyl ether (Polyoxyethylene Polyoxypropylene Alkyl Ether), Polyoxyethylene Castor ether and Polyoxyethylene 1,1,3,3-tetramethylbutyl phenyl ether The composition of any one or more selected from the group.
제 1 항에 있어서,
식물추출물 및 식물성 오일의 중량 합계는 조성물의 총 중량 대비 10 - 70 중량%인 것을 특징으로 하는 조성물.
The method of claim 1,
The total weight of the plant extract and the vegetable oil is 10 to 70% by weight relative to the total weight of the composition.
제 3 항에 있어서,
분산제, 산화방지제 및 제제화용 첨가제는 각각 조성물의 총 중량 대비 1 - 20 중량%, 20 - 60 중량%인 것을 특징으로 하는 조성물.
The method of claim 3, wherein
The dispersant, antioxidant and formulating additive are 1-20% by weight and 20-60% by weight, respectively, relative to the total weight of the composition.
제 1 항에 있어서,
상기 잔디 병해는 갈색퍼짐병 또는 갈색마름병인 것을 특징으로 하는 조성물.

The method of claim 1,
The grass disease is characterized in that the brown spread disease or brown blight disease.

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