CN102805114B - Botanical natural fungicide for fungus and preparation method thereof - Google Patents
Botanical natural fungicide for fungus and preparation method thereof Download PDFInfo
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- CN102805114B CN102805114B CN201210314447.7A CN201210314447A CN102805114B CN 102805114 B CN102805114 B CN 102805114B CN 201210314447 A CN201210314447 A CN 201210314447A CN 102805114 B CN102805114 B CN 102805114B
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Abstract
The invention provides botanical natural fungicide for fungus. Raw material in the fungicide comprises galic essential oil, plant essential oil mixture, organic solvent, surface active agent and water. The fungicide takes the galic essential oil as the main fungicide, comprises more than two types of other plant essential oil as synergistic agent, and can improve the fungus killing efficiency. The fungicide can still achieve the purpose of preventing and controlling fungal disease in the condition that the use level is reduced through the combination of different chemical compositions of the plant essential oil, and can also prevent the probability that one or more types of chemical compounds produce drug fastness. The fungicide has complex chemical compositions and function groups formed by various chemical compositions, and not only improves the action efficiency and increases the synergic action modes, but also increases the penetrability of the chemical compositions and improves action durability of the combination. The chemical compositions are from natural medicinal plants, innoxious to people and animals, and safe to the environment.
Description
Technical field
The invention belongs to bactericide field, be specifically related to a kind of plant source natural bactericidal agent for fungi, in the raw material of described bactericide, contain galic essential oil, plants essential oil mixture, organic solvent, surfactant, water; This bactericide is nontoxic to people and animals, environmentally safe.
Background technology
At present, the annual expense of pest control with insecticide agent to preventing and controlling pest of using in the whole world is up to 10,000,000,000 dollars, and even so, the loss that annual insect pest causes still accounts for 20-30 % of total output of crops.Yet, because the long-term chemical pesticide that uses brings environmental pollution and person poultry poisoning, the series of problems such as sick worm pesticide resistance raising, some countries have started chemical pesticide to take to limit the use of and forbidding measure.Particularly 1992, world environments proposes " will control in the world sales volume and the use of chemical pesticide, the output of biopesticide in 2000 should account for 60 % of agricultural chemicals total amount " with development conference, and afterwards, each state all strengthened development and the exploitation of biopesticide.
The research and development of pure natural plant source pesticide becomes emphasis and the focus of world gradually.Since 20 century 70s, countries in the world government has started to pay attention to research, safe, harmless, efficient, the multi-functional botanical pesticide of development and application.According to the literature: countries in the world scientist is studied the desinsection of 2000 various plants, mildew-resistant, weeding and growth promotion bioactive ingredients, being wherein no lack of is traditional Chinese herbal medicine.These Chinese herbal medicines mainly comprise: Meliaceae (printing chinaberry, melia toosendan), composite family (Dalmatian chrysanthemum), pulse family (trifoliate jewelvine, Sophora alopecuroide), Ericaceae (Azalea pontica), Celastraceae (thunder godvine), Thymelaeceae (root of langdu), Labiatae (aldehydes matter), Lauraceae (camphor tree) etc.
So far, there is portioned product listing.As Dutch Ropapharm B. V. I. O. with Orv oil and thyme wet goods vegetable oil by the composite preparation that obtains, be used for killing the sanitary insect pest effect highly significants such as plant pest and mosquito, fly, and this medicine is better than take lindane as basic chemical insecticide, humans and animals is not had to toxic and side effect, be subject to the welcome of countries in the world, in the registered use of China." Frontline " botanical pesticide preparation of Germany's plant pesticide exploitation, the insect-piebald worm strong and crushing large to pesticide resistance in vegetables, has desirable control efficiency.
Botanical pesticide exploitation both at home and abroad is is mainly researched and developed from two innovation focuses:
The one, from traditional vegetable drug, extract desinsection, fungicidal activity position (composition), as host, be mixed with non-harmful agricultural chemicals.Studying many plants has: composite family, Meliaceae, Celastraceae, Ericaceae, Thymelaeceae and mineral humic acid etc.As proposed pyrethrins, isolate nicotine from tobacco leaf, isolate stemonine etc. from the tuber of stemona from composite family.At present, China's botanical pesticide insecticide registered and that produce has: nicotine, galic essential oil, rotenone, pyrethrins, stemonine, chinaberry element, return artemisin, jervine, camptothecine and humic acid with and the about kind more than 30 of derivative;
The 2nd, from miscellaneous medicinal plant, isolate the active substance of desinsection, sterilization, make the derivative with insecticidal activity, or carry out chemical constitution transformation as template, by the research of structure-activity relationship and insecticidal mechanism, carry out bionical synthetic, formulate out the agricultural chemicals of high-efficiency low-toxicity, as from feverfew, isolated pyrethrins, by anti-intercrescence, become research and production to go out more than 20 kind of pyrethroid, be widely used in crops, forestry, flowers and domestic hygiene insect, play significant control efficiency.Dan China resources of medicinal plant is abundant, and the plant of sterilization and disinsection approximately has kind more than 4000, and exploitation botanical pesticide is had to advantageous advantage.Therefore, in China's research and development, there is efficiency height and the low botanical biological pesticide of cost, there is great Social benefit and economic benefit.
But there are two obvious problems in frequently seen plants source pesticide and chemical pesticide in the market in market competition: the one, drug effect is slow, and the drug effect phase is short, and fungi-proofing insecticide efficiency is low; The 2nd, production cost is high.These two effects limit the industrialized development of botanical pesticide, in production practices, need to solve the efficient and long-acting problem of existing product, and to person poultry harmless, the environment amenable plant source natural bactericidal agent for fungi
Summary of the invention
The invention provides a kind of plant source natural bactericidal agent for fungi, in the raw material of described bactericide, contain galic essential oil, plants essential oil mixture, organic solvent, surfactant, water; Drug effect of the present invention is fast, the drug effect phase is long, antifungal efficiency is high, and to person poultry harmless, environmentally friendly.
The object of the invention is to be achieved through the following technical solutions:
For a plant source natural bactericidal agent for fungi, in the raw material of described bactericide, contain galic essential oil, plants essential oil mixture, organic solvent, surfactant, water, the volume ratio of each raw material of described bactericide is:
Galic essential oil 10-20,
Plants essential oil mixture 100,
Organic solvent 200-250,
Surfactant 100-300,
Water 700-2500;
Further, described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 1-2:1-2:1:1:1:1-2.
Further, described organic solvent is one or more the mixture in methyl alcohol, ethanol, acetone, ethyl acetate, cyclohexanone, methyl oleate, n-butanol, dimethyl formamide.
Further, described surfactant is one or more the mixture in alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog thereof etc.
Further, described surfactant is polyethenoxy sorbitan oleate.
The object of the invention is to realize by following another technical scheme:
A preparation method for the plant source natural bactericidal agent of fungi, contains galic essential oil, plants essential oil mixture, organic solvent, surfactant, water in the raw material of described bactericide, the volume ratio of each raw material of described bactericide is:
Galic essential oil 10-20,
Plants essential oil mixture 100,
Organic solvent 200-250,
Surfactant 100-300,
Water 700-2500;
The preparation method of the described plant source natural bactericidal agent for fungi comprises the steps:
A, described galic essential oil is mixed with blend of essential oils, obtain mixture A; Again described mixture A is dissolved in to described organic solvent, obtains mixture B;
Described blend of essential oils is mixed and is formed by thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, the cinnamon oil of said ratio;
B, described surfactant and water are mixed, obtain solvent C;
C, described mixture B is mixed with described solvent C, carry out stir process, obtain the described plant source natural bactericidal agent for fungi;
In described stir process, temperature is 50-80 degree Celsius, and rotating speed is 1000-4000r/min, and the time is 10-40min.
The present invention has following beneficial effect compared to existing technology:
Because galic essential oil is as main bactericide in the present invention, comprise that two or more other plant essential oils are as synergist, so the efficiency of the killing fungus that the present invention can improve.
Because of the present invention, be by the combination of plants essential oil different chemical composition, so can reduce under the condition of consumption, still can reach the object of prevention and control fungal disease, also can prevent the possibility that single one or several compound develops immunity to drugs.
Because complex chemical composition of the present invention, and the function group that has several types chemical composition, had both improved the efficiency of effect, had increased more synergistic mode, also increased the permeability of chemical composition, so the present invention has improved the lasting medicine of said composition on crop.
Because chemical composition of the present invention derives from natural medicinal plant, belong to food or food additives, so the present invention is nontoxic to people and animals, environmentally safe.
Embodiment
Embodiment 1:
For a plant source natural bactericidal agent for fungi, in the raw material of described bactericide, contain galic essential oil, plants essential oil mixture, organic solvent, surfactant, water, the volume ratio of each raw material of described bactericide is:
Galic essential oil 10-20,
Plants essential oil mixture 100,
Organic solvent 200-250,
Surfactant 100-300,
Water 700-2500;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 1-2:1-2:1:1:1:1-2.
Described organic solvent is one or more the mixture in methyl alcohol, ethanol, acetone, ethyl acetate, cyclohexanone, methyl oleate, n-butanol, dimethyl formamide.
Described surfactant is one or more the mixture in alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog thereof etc.
Described surfactant is polyethenoxy sorbitan oleate.
The preparation method of the described plant source natural bactericidal agent for fungi comprises the steps:
A, described galic essential oil is mixed with blend of essential oils, obtain mixture A; Again described mixture A is dissolved in to described organic solvent, obtains mixture B;
Described blend of essential oils is mixed and is formed by thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, the cinnamon oil of said ratio;
B, described surfactant and water are mixed, obtain solvent C;
C, described mixture B is mixed with described solvent C, carry out stir process, obtain the described plant source natural bactericidal agent for fungi;
In described stir process, temperature is 50-80 degree Celsius,
be preferably 60 degrees Celsius,rotating speed is 1000-4000r/min,
be preferably 2000r/min,time is 10-40min
, be preferably 20min.
Because galic essential oil is as main bactericide in the present embodiment, comprise that two or more other plant essential oils are as synergist, so the efficiency of the killing fungus that the present embodiment can improve.
Because the present embodiment is by the combination of plants essential oil different chemical composition, so can reduce under the condition of consumption, still can reach the object of prevention and control fungal disease, also can prevent the possibility that single one or several compound develops immunity to drugs.
Because the complex chemical composition of the present embodiment, and the function group that has several types chemical composition, had both improved the efficiency of effect, had increased more synergistic mode, also increased the permeability of chemical composition, so the present embodiment has improved the lasting medicine of said composition on crop.
Because the chemical composition of the present embodiment derives from natural medicinal plant, belong to food or food additives, so the present embodiment is nontoxic to people and animals, environmentally safe.
Thyme linaloe oil described in the present embodiment, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil respectively from labiate thyme (
thymus mongolicus) enter the florescence acrial part, Magnoliacea plant Chinese anise (
fructus Anisi Stellati) fresh branches and leaves or ripening fruits, blue gum (
eucalyptus Globulus) or folium eucalypti tree (
eucalyptus globulus) etc. Ye Hezhi, by pinaceae plant masson pine (
pinus massoniana Lamb) or Korean pine (
pinus koraiensis) etc. resin (rosin), the canella fruit of a cubeb litsea tree (
litsea cubeba) cauline leaf or fruit, canella Chinese cassia tree (
cinnamomum cassia) dry branch or leaf in extract.Above-mentioned thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil are purchased from Ji'an City Qingyuan District magnificent natural perfume material oil refinery.
The present embodiment volume unit used is milliliter, also can be for rising.
Water described in the present embodiment is deionized water.
Each raw material described in the present embodiment is the conventional products of selling on market, and the equipment using in the present embodiment is also the conventional products of selling on market.
In the present embodiment, each raw material in described can be in the ratio range providing flexible combination, at this, do not enumerate one by one.
Embodiment 2:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 20,
Plants essential oil mixture 100,
Organic solvent 240,
Surfactant 240,
Water 2160;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:2:1:1:1:1;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment is as follows for the inhibiting test data of frequently seen plants fungi.
For trying bactericide: the bactericide of the present embodiment
For trying bacterial classification: Sclerotinia sclerotiorum
sclerotinia sclerotiorum, cucumber fusarium axysporum
fusarium oxysporum f.sp cucumerinum, tomato early blight bacterium
alternaria solaniwith shredded chili pyrenomycetes
rhizoctonia solani, tomato wilt germ
fusarium oxysporum f.sp. Lycopersici, the cladosporium leaf and fruit mould of tomato germ (Fulvia fulva of Deuteromycotina
fulvia fulva (Cooke) Cif) 6 kinds.
Test method: adopt biomass growth rate method.Under aseptic condition, after the medicament accurately weighing is mixed with the quantitative culture medium of sterilizing, pour in culture dish, make flat board, every concentration repeats 3 times, with diameter 9mm card punch, at cultured colony edge, cut bacterium cake, with sterilization transfer needle, the reversion of bacterium cake is transplanted on pastille medium, is placed in constant temperature culture under 25 ℃ of conditions and, after 7 days, with ruler, measures bacterium cake expansion diameter, contrast bacterium colony expansion diameter with not dispenser and compare, obtain medicament and suppress growth rate.
Inhibiting rate (%)=[(control group bacterium colony average diameter-pastille group bacterium colony average diameter)/control group bacterium colony expansion average diameter] * 100%
Experimental result:
Table 1 botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 86.1 |
| Cucumber fusarium axysporum | 100 | 100 | 100 | 82.4 |
| Tomato early blight bacterium | 100 | 100 | 90.8 | 74.6 |
| Shredded chili pyrenomycetes | 100 | 100 | 91.7 | 77.9 |
| Tomato wilt germ | 100 | 91.3 | 77.9 | 52.7 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 87.3 |
From table 1, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Embodiment 3:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 10,
Plants essential oil mixture 100,
Organic solvent 220,
Surfactant 165,
Water 935;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:2:1:1:1:1;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment for the inhibiting test method of frequently seen plants fungi referring to embodiment 2, for the test data of the inhibiting rate of frequently seen plants fungi as table 2.
Table 2: botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 85.7 |
| Cucumber fusarium axysporum | 100 | 100 | 96.5 | 83.4 |
| Tomato early blight bacterium | 100 | 100 | 94.3 | 76.8 |
| Shredded chili pyrenomycetes | 100 | 100 | 92.2 | 76.9 |
| Tomato wilt germ | 100 | 93.6 | 75.8 | 52.8 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 87.3 |
From table 2, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Embodiment 4:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 15,
Plants essential oil mixture 100,
Organic solvent 230,
Surfactant 200,
Water 1334;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment for the inhibiting test method of frequently seen plants fungi referring to embodiment 2, for the test data of the inhibiting rate of frequently seen plants fungi as table 3.
Table 3: botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 85.7 |
| Cucumber fusarium axysporum | 100 | 100 | 96.8 | 82.4 |
| Tomato early blight bacterium | 100 | 100 | 92.5 | 76.8 |
| Shredded chili pyrenomycetes | 100 | 100 | 92.9 | 77.9 |
| Tomato wilt germ | 100 | 91.3 | 75.8 | 53.8 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 88.0 |
From table 3, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Embodiment 5:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 20,
Plants essential oil mixture 100,
Organic solvent 240,
Surfactant 240,
Water 2160;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:1:1:1:1:2;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment for the inhibiting test method of frequently seen plants fungi referring to embodiment 2, for the test data of the inhibiting rate of frequently seen plants fungi as table 4.
Table 4: botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 83.5 |
| Cucumber fusarium axysporum | 100 | 100 | 96.5 | 81.7 |
| Tomato early blight bacterium | 100 | 100 | 94.3 | 76.8 |
| Shredded chili pyrenomycetes | 100 | 100 | 93.1 | 74.5 |
| Tomato wilt germ | 100 | 94.5 | 76.4 | 50.6 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 87.5 |
From table 4, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Embodiment 6:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 10,
Plants essential oil mixture 100,
Organic solvent 220,
Surfactant 165,
Water 935;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:1:1:1:1:2;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment for the inhibiting test method of frequently seen plants fungi referring to embodiment 2, for the test data of the inhibiting rate of frequently seen plants fungi as table 5.
Table 5: botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 86.1 |
| Cucumber fusarium axysporum | 100 | 100 | 100 | 82.4 |
| Tomato early blight bacterium | 100 | 100 | 90.8 | 74.6 |
| Shredded chili pyrenomycetes | 100 | 100 | 91.7 | 77.9 |
| Tomato wilt germ | 100 | 91.3 | 77.9 | 52.7 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 87.3 |
From table 5, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Embodiment 7:
The present embodiment is the preferred version on embodiment 1 basis, and raw materials used quality is identical with embodiment 1, and the volume ratio of the raw material that should comprise for the plant source natural bactericidal agent of fungi is:
Galic essential oil 15,
Plants essential oil mixture 100,
Organic solvent 230,
Surfactant 220,
Water 1334;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:1:1:1:1:2;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
The present embodiment volume unit used is milliliter, also can be for rising.
The preparation method of the present embodiment is referring to embodiment 1.
The present embodiment for the inhibiting test method of frequently seen plants fungi referring to embodiment 2, for the test data of the inhibiting rate of frequently seen plants fungi as table 6.
Table 6: botanical fungicide is to the inhibitory action of plant epiphyte (%)
| Plant epiphyte | 500 times | 1000 times | 1500 | 2000 times |
| Sclerotinia sclerotiorum | 100 | 100 | 100 | 88.5 |
| Cucumber fusarium axysporum | 100 | 100 | 100 | 85.4 |
| Tomato early blight bacterium | 100 | 100 | 93.1 | 77.2 |
| Shredded chili pyrenomycetes | 100 | 100 | 92.3 | 79.0 |
| Tomato wilt germ | 100 | 100 | 80.5 | 55.7 |
| Cladosporium leaf and fruit mould of tomato germ | 100 | 100 | 100 | 87.5 |
From table 6, analyze, the bactericide of the present embodiment, in certain concentration range, has stronger inhibitory action to different plant pathogenic fungis, and is enhancing trend with the increase inhibitory action of concentration.
Claims (8)
1. a plant source natural bactericidal agent is for the purposes of Antifungi, it is characterized in that: in the raw material of described bactericide, contain galic essential oil, plants essential oil mixture, organic solvent, surfactant, water, the volume ratio of each raw material of described bactericide is:
Galic essential oil 10-20,
Plants essential oil mixture 100,
Organic solvent 200-250,
Surfactant 100-300,
Water 700-2500;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 1-2:1-2:1:1:1:1-2;
The preparation method of described plant source natural bactericidal agent comprises the steps:
A, described galic essential oil is mixed with blend of essential oils, obtain mixture A; Again described mixture A is dissolved in to described organic solvent, obtains mixture B;
B, described surfactant and water are mixed, obtain solvent C;
C, described mixture B is mixed with described solvent C, carry out stir process, obtain the described plant source natural bactericidal agent for fungi;
In described stir process, temperature is 50-80 degree Celsius, and rotating speed is 1000-4000r/min, and the time is 10-40min.
2. plant source natural bactericidal agent according to claim 1, for the purposes of Antifungi, is characterized in that: described organic solvent is one or more the mixture in methyl alcohol, ethanol, acetone, ethyl acetate, cyclohexanone, methyl oleate, n-butanol, dimethyl formamide.
3. plant source natural bactericidal agent according to claim 1 and 2, for the purposes of Antifungi, is characterized in that:
Described surfactant is one or more the mixture in alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers.
4. plant source natural bactericidal agent according to claim 3, for the purposes of Antifungi, is characterized in that: described surfactant is polyethenoxy sorbitan oleate.
5. plant source natural bactericidal agent according to claim 1 and 2, for the purposes of Antifungi, is characterized in that:
The volume ratio of each raw material of described bactericide is:
Galic essential oil 20,
Plants essential oil mixture 100,
Organic solvent 240,
Surfactant 240,
Water 2160;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:2:1:1:1:1;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
6. plant source natural bactericidal agent according to claim 1 and 2, for the purposes of Antifungi, is characterized in that:
The volume ratio of each raw material of described bactericide is:
Galic essential oil 10,
Plants essential oil mixture 100,
Organic solvent 220,
Surfactant 165,
Water 935;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:2:1:1:1:1;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
7. plant source natural bactericidal agent according to claim 1 and 2, for the purposes of Antifungi, is characterized in that:
The volume ratio of each raw material of described bactericide is:
Galic essential oil 15,
Plants essential oil mixture 100,
Organic solvent 230,
Surfactant 200,
Water 1334;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:2:1:1:1:1;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
8. plant source natural bactericidal agent according to claim 1 and 2, for the purposes of Antifungi, is characterized in that:
The volume ratio of each raw material of described bactericide is:
Galic essential oil 20,
Plants essential oil mixture 100,
Organic solvent 240,
Surfactant 240,
Water 2160;
Described plants essential oil mixture comprises thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil, and the volume ratio of described thyme linaloe oil, oil of badian, eucalyptus oil, turpentine oil, litsea cubeba oil, cinnamon oil is 2:1:1:1:1:2;
Described organic solvent is ethanol, and described surfactant is polyethenoxy sorbitan oleate.
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| CN110623005B (en) * | 2019-09-16 | 2021-06-29 | 江西省农业科学院蔬菜花卉研究所 | Compound plant extract for inhibiting bitter gourd fusarium wilt and preparation method thereof |
| CN115851133A (en) * | 2022-12-14 | 2023-03-28 | 沈阳盛达惠发化工有限公司 | Insect-proof scratch-resistant composite paint for solid wood furniture and preparation and use methods thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1170504A (en) * | 1997-07-02 | 1998-01-21 | 刘丰 | Plant pesticide |
| CN1286031A (en) * | 1999-08-28 | 2001-03-07 | 冼启福 | Vegetative insecticidal germicidal agent |
| WO2004034791A1 (en) * | 2002-10-21 | 2004-04-29 | Givaudan Sa | Pesticidal compositions |
| CN101920028A (en) * | 2009-06-16 | 2010-12-22 | 杜文广 | Air fresher with sterilization function |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034707C (en) * | 1992-03-30 | 1997-04-30 | 王金定 | Fourth generation vegetable germicide for agriculture |
| CN1172598C (en) * | 2000-10-23 | 2004-10-27 | 邓广启 | Bactericidal and pesticidal garlic oil emulsion and its prepn. |
| CN101385474A (en) * | 2007-12-27 | 2009-03-18 | 广东省中药研究所 | Plant source natural bactericidal agent |
| CN101223891A (en) * | 2008-01-25 | 2008-07-23 | 中国农业大学 | Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof |
-
2012
- 2012-08-30 CN CN201210314447.7A patent/CN102805114B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1170504A (en) * | 1997-07-02 | 1998-01-21 | 刘丰 | Plant pesticide |
| CN1286031A (en) * | 1999-08-28 | 2001-03-07 | 冼启福 | Vegetative insecticidal germicidal agent |
| WO2004034791A1 (en) * | 2002-10-21 | 2004-04-29 | Givaudan Sa | Pesticidal compositions |
| CN101920028A (en) * | 2009-06-16 | 2010-12-22 | 杜文广 | Air fresher with sterilization function |
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