CN101755847B - Metarhiziumanisopliae compound insecticide composition and use thereof - Google Patents
Metarhiziumanisopliae compound insecticide composition and use thereof Download PDFInfo
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Abstract
The invention relates to a metarhiziumanisopliae compound insecticide composition and use thereof. The metarhiziumanisopliae compound insecticide composition is characterized in that: the active ingredients of the composition include metarhiziumanisopliae and a new neonicotinoid insecticide; the density of the metarhiziumanisopliae in the composition is 1 to 10 billion spores per gram; and the new neonicotinoid insecticide content of the composition is 20 mass percent; and the rest content of the composite are pesticide auxiliary ingredients. The composition has an obvious synergistic effect on grubs and is suitable for preventing and controlling plant pests, particularly grubs. The composition can be used on various crops such as lawns, peanut, sweet potatoes and vegetables or in various places and has the advantages of stable insecticide effect, high quick-acting performance, long efficacy duration, safety and environmental protection.
Description
Technical field
The present invention relates to a kind of composition pesticide and application thereof, especially a kind of application that contains complex insecticidal composition and the control crop pest thereof of green muscardine fungus.
Background technology
Grub is the general name of scarab beetle larva, belongs to coleoptera, Scarabaeoidea, and the soybean of causing harm, peanut, sugarcane, corn and some traditional Chinese medicines, herbage, flowers, sapling, artificial lawn etc., wherein serious with the peanut of causing harm.In recent years, along with the adjustment of pattern of farming, the change of crop allocation and the variation of weather, grub was taken place on a large scale in China year after year, and wildness causes harm.The history of life of grub is longer, accomplishes a generation required time 1-6 and does not wait, and in the history of life, larval phase is lasted the longest, and because it causes harm the quite difficulty so control is got up underground.For the control of grub is main with chemical pesticide still mainly, usually uses chemical pesticide in a large number, the highly toxic pesticide that has even use high malicious high residue, contaminated environment, killed natural enemies and other beneficial organism and cause insect wildness and pesticide resistance more easily.
Green muscardine fungus genus Fungi Imperfecti, clump stalk Zoopagales, the mould section of clump stalk, green muscardine fungus genus; It is a kind of insect pathogenic bacteria of wide spectrum; Have certain selectivity,, also have free from environmental pollution, noresidue, insect advantage such as can not develop immunity to drugs simultaneously the person poultry harmless; The area of using its pest control has abroad surpassed white muscardine fungi, and control efficiency can match in excellence or beauty with white muscardine fungi.At present, the green muscardine fungus bacterial strain that has has been developed as fungus insecticide, and has registered, and is used for beach control migratory locusts like 10,000,000,000 spores/milliliter Metarhizium anisopliae oil-suspending agent.
Independent use green muscardine fungus effect is slow and preventive effect is unstable; Green muscardine fungus and decis (decis), phoxim, the young urea III that goes out etc. use with and can improve desinsection speed and [see: Fujian Forestry Inst.'s journal 2001; 21 (4): 308～311]; Also existing green muscardine fungus and pyrethrin pesticide are processed the report of composition, but general chemical agent survives to spore and sprout influentially, be difficult to process stable mix preparation.
Summary of the invention
The complex composition that the purpose of this invention is to provide a kind of green muscardine fungus and chemical pesticide, composition not only can improve desinsection speed and effect, and stability be significantly increased, use more convenient, cost is lower; Another purpose of the present invention provides the method for a kind of composition for preventing and controlling soil insect grub with green muscardine fungus and anabasine insecticide.
For solving the problems of the technologies described above, the inventor finds that through a large amount of biologicall test screenings green muscardine fungus and anabasine insecticide are composite with certain proportion, and grub is had obvious synergistic effect.On the basis of above-mentioned discovery,, formed technical scheme of the present invention, that is: through a large amount of formulation screening tests
A kind of green muscardine fungus complex insecticidal composition is characterized in that, its active ingredient is Metarhizium anisopliae and thiophene worm piperazine.
The content of green muscardine fungus is 1～10,000,000,000 spore/gram in the composition, and the content of thiophene worm piperazine is 10～200 gram/kilograms, and all the other are the agricultural chemicals auxiliary element.Said green muscardine fungus is the natural separation bacterial strain of Metarhizium anisopliae (Metarhizium anisopliae) and through the mutation of artificial culture; The vegetative mycelium that available its liquid fermentation produces; The preparation of conidium powder that the conidium that also available solid fermentation produces, the present composition preferably adopt solid fermentation to produce.As a kind of improvement to green muscardine fungus Pesticidal combination of the present invention, the content of green muscardine fungus is preferably 5～5,000,000,000 spore/grams in the composition.
Said anabasine insecticide is any one in Imidacloprid, thiophene worm piperazine, thiophene worm amine, the Acetamiprid.As a kind of improvement to green muscardine fungus Pesticidal combination of the present invention, the content of anabasine insecticide is preferably 20～100 gram/kilograms in the composition.
Composition of the present invention is except that active ingredient green muscardine fungus and anabasine insecticide; Also include and benefit active ingredient stable auxiliary element of bringing into play with drug effect in preparation; So that process the formulation that is fit to use; Such as, can be prepared into wetting powder, granule, water dispersible granules or oil-suspending agent etc. with known method.
Said auxiliary element comprises that dispersant, wetting agent, emulsifier, stabilizing agent, antifreezing agent, solvent, filler etc. and other are of value to the known substance that active ingredient is stablized and drug effect is brought into play in preparation; All be the various compositions of using or allow use in the pesticidal preparations always; Do not have special qualification, concrete composition and consumption require to confirm through simple experiment according to prescription.
The dispersant that is applicable to preparation present composition wetting powder and water dispersible granules comprises lignosulfonates (like sodium lignin sulfonate), modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensate sulfonates, AEO, nekal, dodecyl polyoxyethylene ether phosphate, benzylphenol APEO, APES, draw back in the powder one or more.
Be applicable to that the wetting agent for preparing present composition wetting powder and water dispersible granules comprises one or more in extraordinary polyether-modified polysiloxane, lauryl sodium sulfate, fatty acid sulphate, AEO, the alkyl phenolic group polyoxyethylene groups ether.
Be applicable to that the disintegrant for preparing present composition water dispersible granules comprises one or more in bentonite, ammonium sulfate, urea, magnesium sulfate, the sucrose.
Be applicable to that the adhesive for preparing present composition granule and water dispersible granules comprises one or more in paraffin oil, polyethylene glycol, polyvinyl alcohol, cyclodextrin, starch, the sodium silicate.
The oil that is applicable to preparation present composition oil-suspending agent comprises rapeseed oil, sesame oil, peanut wet goods vegetable oil and paraffin oil; Emulsifier can be selected one or more in sorbitan monooleate, sorbitan fatty acid ester APEO, fatty alcohol and ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine list olein, castor oil and the ethylene oxide condensate for use.
Can add antioxidant or anti ultraviolet agent in the present composition preparation as stabilizing agent; Available composition comprises butylated hydroxyarisol (BHA), 2; In 6-BHT (BHT), n-propyl gallate, the ditert-butylhydro quinone etc. one or more; Usage amount is the 0%-5% of total formulation weight amount, is preferably 1%-3%.
Green muscardine fungus complex insecticidal composition of the present invention can be used for preventing and treating ground or the soil insect of harm plant, like aphid, thrips, grub, wireworm etc.The present composition is particularly suitable for preventing and treating the various grubs of harm foot end, and suitable place or plant comprise lawn, peanut, sweet potato, vegetables etc.
Application method can be taked to spray, pouring is irritated, spread fertilizer over the fields etc., and above application method all can combine with the further mixed soil phase.It is better to use present composition effect in grub 1-2 Sheng in the age phase, and formulation rate is according to differently with conditions such as soil, crops confirming through simple experiment in worm kind, insect density, worm age.
The present composition has the following advantages: after two kinds of combination of active principles grub is had synergistic effect; Relatively use conveniently with green muscardine fungus with single, efficacy stability, quick-acting is good; Compare with chemical pesticide with single, the lasting period is long, reduces the usage amount of chemical pesticide, reduces environmental pollution; The mechanism of action of composition active principle is different, and insect is not easy to develop immunity to drugs.
Embodiment
Composition of the present invention can be prepared into known method and be fit to the formulation that agricultural is used, and reasonable formulation is wetting powder, granule, water dispersible granules or oil-suspending agent.
In order to make the object of the invention, technical scheme and advantage clearer, the present invention further describes with following specific embodiment and test data, but the present invention is limited to these examples absolutely not.The following stated muscardine spore all refers to the Metarhizium anisopliae conidium, and the amount of said anabasine insecticide is effective dose, converts according to used former medicine content during actual the preparation, and the amount of deviation is regulated through filling adding amount.
Embodiment 1: green muscardine fungus.Acetamiprid granule (green muscardine fungus 100,000,000 spores/g+ Acetamiprid 5g/kg)
Take by weighing Metarhizium anisopliae (R8-4 bacterial strain; The Chinese Academy of Agricultural Sciences's plant protection Research Institute) the high cryptogam 20g of 5,000,000,000 spores/g, Acetamiprid 5g, paraffin oil 10g, corn cob granule 965g; Acetamiprid is mixed with corn cob granule with small amount of acetone dissolving back; In blender, the muscardine spore powder is mixed with corn cob granule again, conidial powder evenly is adsorbed on the corn cob granule, under agitation spray into paraffin oil sealing at last to reduce conidial powder and come off and to reduce suction.
Embodiment 2: green muscardine fungus.Imidacloprid wettable powder (green muscardine fungus 10,000,000,000 spores/g+ Imidacloprid 100g/kg)
Take by weighing Metarhizium anisopliae (Chongqing Chongda Bio-tech. Development Co Ltd) 20,000,000,000 spores/high cryptogam 500g of g, Imidacloprid 100g, calcium lignosulfonate 30g, lauryl sodium sulfate 10g, 2; 6-BHT 20g, kaolin 340g; With Imidacloprid, calcium lignosulfonate, lauryl sodium sulfate, 2; 6-BHT and kaolin mix the back and make superfine powder with the airslide disintegrating mill pulverizing, mix with the muscardine spore powder and promptly get product.
Embodiment 3: green muscardine fungus.Imidacloprid wettable powder (green muscardine fungus 4,000,000,000 spores/g+ Imidacloprid 80g/kg)
Take by weighing Metarhizium anisopliae (R8-4 bacterial strain; The Chinese Academy of Agricultural Sciences's plant protection Research Institute) the high cryptogam 400g of 10,000,000,000 spores/g, Imidacloprid 80g, alkyl naphthalene formaldehyde condensate sulfonates 30g, AEO 10g, butylated hydroxyarisol 20g, diatomite 460g; Imidacloprid, alkyl naphthalene formaldehyde condensate sulfonates, AEO, butylated hydroxyarisol and diatomite are mixed the back make superfine powder, mix with the muscardine spore powder again and promptly get product with the airslide disintegrating mill pulverizing.
Embodiment 4: green muscardine fungus.Thiophene worm piperazine water dispersible granules (green muscardine fungus 5,000,000,000 spores/g+ thiophene worm piperazine 200g/kg)
Take by weighing Metarhizium anisopliae (R8-4 bacterial strain; The Chinese Academy of Agricultural Sciences's plant protection Research Institute) the high cryptogam 500g of 10,000,000,000 spores/g, thiophene worm piperazine 80g, alkyl naphthalene formaldehyde condensate sulfonates 50g, AEO 20g, butylated hydroxyarisol 50g, ammonium sulfate 20g, starch 10, diatomite 270g; After thiophene worm piperazine, alkyl naphthalene formaldehyde condensate sulfonates, AEO, butylated hydroxyarisol, ammonium sulfate and diatomite mixing coarse crushing; Make superfine powder with the airslide disintegrating mill pulverizing; Is that binding agent carry out granulation with the starch solution with the gained superfine powder in fluidized bed pelletizer, drying after vibratory sieve sieve product.
Embodiment 5: green muscardine fungus.Imidacloprid oil-suspending agent (green muscardine fungus 3,000,000,000 spores/g+ Imidacloprid 20g/kg)
Take by weighing Metarhizium anisopliae (Chongqing Chongda Bio-tech. Development Co Ltd) 20,000,000,000 spores/high cryptogam 150g of g, Imidacloprid 20g, sorbitan fatty acid ester APEO 100g, calcium dodecyl benzene sulfonate 20g, butylated hydroxyarisol 30g, peanut oil 380g, sesame oil 300g; Successively add in mixing kettle in the ratio of filling a prescription Imidacloprid, sorbitan fatty acid ester APEO, calcium dodecyl benzene sulfonate, butylated hydroxyarisol and vegetable oil; Stir; Get into sand mill again and carry out sand milling, sand milling to 90% particle diameter promptly gets the oil-suspending agent product less than mixing with the muscardine spore powder after 5 microns.
Embodiment 6: green muscardine fungus.Thiophene worm amine oil-suspending agent (green muscardine fungus 1,000,000,000 spores/g+ thiophene worm amine 50g/kg)
Take by weighing Metarhizium anisopliae (R8-4 bacterial strain; The Chinese Academy of Agricultural Sciences's plant protection Research Institute) how sodium sulfonate 20g, n-propyl gallate 50g, rapeseed oil 660g of the high cryptogam 100g of 10,000,000,000 spores/g, thiophene worm amine 50g, sorbitan monooleate 120g, methylene; How sodium sulfonate, n-propyl gallate and rapeseed oil add in the mixing kettle in the ratio of prescription successively with thiophene worm amine, sorbitan monooleate, methylene; Stir; Get into sand mill again and carry out sand milling, sand milling to 90% particle diameter promptly gets the oil-suspending agent product less than mixing with the muscardine spore powder after 5 microns.
The biologicall test Test Example:
The agricultural chemicals of different cultivars shows three kinds of type of action after mixing usually, and promptly summation action, synergistic effect and antagonism act on but which kind of is specially, and have only through a large amount of tests and just can know.The prescription of compound synergic can obviously improve actual control efficiency, reduces the usage amount of agricultural chemicals, helps to delay the generation speed of pest resistance to insecticide.
Applicant of the present invention is through a large amount of screening tests; Unexpected discovery green muscardine fungus and anabasine insecticide are composite with certain proportion; Grub is had obvious synergistic effect, and be not only the simple addition of two kinds of medicaments, this can know from following biologicall test instance and find out.
Test medicine: Metarhizium anisopliae (R8-4 bacterial strain, the Chinese Academy of Agricultural Sciences's plant protection Research Institute) the high cryptogam of 10,000,000,000 spores/g; The former medicine of 95% Imidacloprid (Jiangsu Changlong Chemical Co); The former medicine of 95.8% thiophene worm piperazine (Switzerland Syngenta Co.,Ltd).
Subjects: anomala corpulenta (Anomala corpulenta Motschulsky) 2 instar larvaes
Bioassay method: adopt infusion process.With the sterile water prepared and diluted muscardine spore suspension that adds 0.05% Tween-80; Anabasine insecticide adds 10% triton x-100 with acetone earlier and is made into mother liquor; Sterile water with 0.05% Tween-80 is diluted to the concentration that needs then, and composition adopts the dilution mixed preparing; Larva is flooded 30s in soup, after unnecessary soup is removed in suction on the aseptic filter paper, put into the culture dish that fills 30g sterilization soil, add 5 raisings of the every ware of carrot slice.Each concentration is established 4 repetitions, and every confession examination borer population that repeats is 10, establishes 0.05% Tween-80 and is contrast.Daily check, write down dead borer population.
The result calculates and evaluation method:
The expection effectiveness of mixture of active principles (be theoretical control efficiency, %) use Colby formula is confirmed and is compared with observed effectiveness:
Colby formula: E=X+Y-X*Y/100
E: the active components A working concentration is a in composition, and the compositions contemplated when active component B working concentration is b is renderd a service,
X: the effectiveness when the active components A using dosage is a,
Y: the effectiveness when active component B using dosage is b.
Explanation has synergistic effect when observed control efficiency surpasses the expection preventive effect.
Green muscardine fungus and Imidacloprid combination are measured the synergistic effect of grub and are seen table 1.
Table 1 green muscardine fungus and Imidacloprid combination are to the synergistic effect of grub
Measuring result's (table 1) shows: green muscardine fungus 1 * 10
5～1 * 10
7Spore/ml and Imidacloprid 10～200mg/L compounded combination are used 8 proportionings, and (M1～M8) the actual observation effectiveness of grub is all renderd a service greater than the theory of pressing the calculating of Colby formula explains that all performance has synergistic effect.
Green muscardine fungus and the combination of thiophene worm piperazine are measured the synergistic effect of grub and are seen table 1.
Table 2 green muscardine fungus and the combination of thiophene worm piperazine are to the synergistic effect of grub
Measuring result's (table 2) shows: green muscardine fungus 1 * 10
5～1 * 10
7Spore/ml and thiophene worm piperazine 10～180mg/L compounded combination are used 8 proportionings, and (M1～M8) the actual observation effectiveness of grub is all renderd a service greater than the theory of pressing the calculating of Colby formula, explaining all has synergistic effect.
Store the active detection of spore:
Peptone potato dextrose agar (PPDA) directly is applied on the slide, gets a little present composition that contains the green muscardine fungus conidial powder again and be diluted to 1 * 10 with the sterile water that contains 0.1% glycerine
7Individual/mL, evenly smear 28 ℃ of 36h that preserve moisture after dripping 20 μ L spore suspensions.Every kind of spore of handling about 300 of statistics, simple microscope is observed down and record spore germination situation for 400 times, calculates spore germination rate.
The bin stability of muscardine spore in table 3 present composition
| ? | The sample moisture content, % | Store the forespore germination rate, % | 180 days spore germination rates of indoor storage below 25 ℃, % |
| Embodiment 1 | 8.6 | 94.7 | 83.0 |
| Embodiment 2 | 7.8 | 95.9 | 86.3 |
| Embodiment 3 | 6.1 | 95.2 | 89.4 |
| Embodiment 4 | 6.4 | 95.7 | 83.9 |
| Embodiment 5 | 4.5 | 96.1 | 90.8 |
| Embodiment 6 | 6.5 | 95.6 | 89.9 |
The field application test:
1, control lawn grub
Press the using dosage of each medicament in the table 4, with court sand (20g/m
2) spread fertilizer over the fields (granule of embodiment 1 directly spreads fertilizer over the fields) with medicament after mixing thoroughly, with chemicals chlopyrifos (40% missible oil) 0.25mL/m
2With clear water is to contrast, and waters once after the dispenser.
7d, 15d, 30d, 60d after preceding 1d of dispenser and the dispenser respectively by 5 samplings, collect 25 * 25cm at every
2All grubs in the 15cm of the sample prescription face of land, record borer population of living, calculate insect population go down rate with proofread and correct preventive effect, with DPS software data are handled.
The field test results of table 4 present composition control lawn grub
Visible by last table result; 7～60d all more than 84%, satisfy lawn insect control requirement fully, and the preventive effect of contrast medicament 40% chlorpyrifos ec after 30 days is just below 50% to the control efficiency of lawn grub behind the embodiment of the invention 1～6 composition medicine; Effect is relatively poor and the longevity of residure short; 7 days preventive effect also about 50%, though long quick-acting of the longevity of residure is poor, can not be controlled harm rapidly behind the processing medicine of the single usefulness of green muscardine fungus.
It is 2000g that 40% chlorpyrifos ec consumption is amounted to per hectare active ingredient; And in the embodiment of the invention chemical constituent anabasine insecticide to amount to per hectare active ingredient be 300～1000g; Be merely below 50% of chlopyrifos; Significantly reduce the usage amount of chemical pesticide like this, helped environmental protection and ecological safety.
2, control peanut grub
Secondly the black greatly gill cockchafer (accounting for 55-60%) of the sociales of the chafer that the test area takes place is Holotrichia parallela and anomala corpulenta.
Test method: adopt the randomized arrangement method to set the sub-district in the field, each sub-district 33m
2, adopting the clear water contrast, each is handled 3 times and repeats.The consumption of behind site preparation and fertilization, before the sowing medicament being set according to test is mixed fine earth (30kg/ mu) ditch and is imposed near the seed furrow (or cave), and degree of depth 10-15cm executes the back earthing.
Investigate altogether three times when peanut is emerged, when ripe mid-term of peanut and results, each sub-district is got 5 points at random, every 10 cave; Investigate for the first time peanut the emerge situation and the growth of cereal crop seedlings; For the second time investigate residual borer population, investigate every cave worm alive, total pod number and killed pod number for the third time, calculate killed fruit rate, control efficiency.
The field test results of table 5 present composition control peanut grub
| Handle | Dosage, g/667m 2 | Average pod rate of damage (%) | Control efficiency (%) |
| Embodiment 3 | 600 | 6.6 | 70.54 |
| Embodiment 4 | 300 | 7.3 | 67.41 |
| Embodiment 5 | 900 | 5.1 | 77.23 |
| Embodiment 6 | 1200 | 4.9 | 78.13 |
| 40% chlorpyrifos ec | 450 | 12.8 | 42.86 |
[0076]
| 10% imidacloprid wettable powder | 1000 | 11.9 | 46.88 |
| 5,000,000,000 spores/g green muscardine fungus wetting powder (R8-4) | 1200 | 9.60 | 57.14 |
| CK | -- | 22.4 | -- |
Table 5 is the result show; Embodiment of the invention composition obviously is better than organophosphorous pesticide 40% chlorpyrifos ec to the preventive effect of peanut grub; Also significantly be superior to the preventive effect of the single agent of Imidacloprid or green muscardine fungus, explain that the present composition has synergistic effect, effective to the control of peanut grub.
Claims (4)
1. a green muscardine fungus complex insecticidal composition is characterized in that, its active ingredient is Metarhizium anisopliae and thiophene worm piperazine, and the content of green muscardine fungus is that the content of 1～10,000,000,000 spore/gram, thiophene worm piperazine is 10～200 gram/kilograms in the composition, and all the other are the agricultural chemicals auxiliary element.
2. Pesticidal combination according to claim 1 is characterized in that, the formulation of composition is wetting powder, granule, water dispersible granules, microcapsules or oil-suspending agent.
3. Pesticidal combination according to claim 2 is characterized in that the formulation of composition is a water dispersible granules, and the content of green muscardine fungus is that the content of 5,000,000,000 spore/grams, thiophene worm piperazine is 200 gram/kilograms in the composition.
4. the described green muscardine fungus complex insecticidal composition of claim 1 is used for control of grubs.
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