CN105432669B - A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid - Google Patents

A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid Download PDF

Info

Publication number
CN105432669B
CN105432669B CN201410414847.4A CN201410414847A CN105432669B CN 105432669 B CN105432669 B CN 105432669B CN 201410414847 A CN201410414847 A CN 201410414847A CN 105432669 B CN105432669 B CN 105432669B
Authority
CN
China
Prior art keywords
alkaloid
muscardine
spore powder
auxiliary agent
carrier
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201410414847.4A
Other languages
Chinese (zh)
Other versions
CN105432669A (en
Inventor
付顺林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huiyuan Luyuan Technology Development Co., Ltd.
Original Assignee
Huiyuan Luyuan Technology Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huiyuan Luyuan Technology Development Co Ltd filed Critical Huiyuan Luyuan Technology Development Co Ltd
Priority to CN201410414847.4A priority Critical patent/CN105432669B/en
Publication of CN105432669A publication Critical patent/CN105432669A/en
Application granted granted Critical
Publication of CN105432669B publication Critical patent/CN105432669B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to biological pesticide technical field, specifically a kind of green muscardine fungus is combined the method for preventing lepidopterous larvae with alkaloid.A kind of insecticidal action with both green muscardine fungus and alkaloid is designed to provide to be combined, method to prevent lepidopterous larvae, it is mainly made of muscardine spore powder and biological base agent, content is muscardine spore powder 35%-60%, alkaloid 35%-50%, auxiliary agent 6%-15%, carrier are 8%-15% compositions;It takes muscardine spore powder, alkaloid, auxiliary agent to be uniformly mixed ratio, crosses airslide disintegrating mill and crush, add filler, be uniformly mixed;Pulverizer crushes, and is sieved by 325 mesh until reaching 98%, diameter is less than 44 microns, and 25 microns of average grain diameter obtains product;It is degradable in the environment, to natural enemy and beneficial organism safety, do not develop immunity to drugs.

Description

A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid
Technical field
The present invention relates to biological pesticide technical field, specifically a kind of green muscardine fungus is combined prevention lepidopterous larvae with alkaloid Method.
Background technology
Metarhizium Fungi Imperfecti, clump stalk Zoopagales, Cong Gengmei sections, Metarhizium, are a kind of insect pathogenic bacterias of wide spectrum, The area of its pest control of foreign applications has been more than muscardine, and control effect can match in excellence or beauty with muscardine.A variety of evils can be parasitized A kind of fungi of worm, is entered by body surface or feeding effect in pest body, in pest body constantly increase breeding by consume nutrition, Mechanical penetration generates toxin, and is constantly propagated in pest population, keeps pest lethal.Green muscardine fungus has certain specificity, right Person poultry harmless, while also having many advantages, such as that free from environmental pollution, noresidue, pest will not develop immunity to drugs.
Alkaloid is a kind of nitrogenous alkalinity being present in nature (predominantly plant, but some exists in animal) Organic compound has the property like alkali, so being also known as counterfeit alkali in the past.Most of cyclic structures for having complexity, nitrogen include more In ring, there is significant bioactivity, is one of active ingredient important in Chinese herbal medicine.With optical activity.Some are free of alkali Property and from plant organic compounds containing nitrogen, have apparent bioactivity, therefore be included in the range of alkaloid.And have Natural organic compounds containing nitrogen, such as certain vitamins, amino acid, peptides are derived from a bit, are traditionally not belonging to " alkaloid " again.
Lepidoptera (scientific name Lepidoptera) includes moth, two class insect of butterfly, and larva is caterpillar, and all kinds of cultivations of causing harm are planted Object, the often feeding of the bodily form the greater blade or brill moth limb to the greatest extent.Bodily form smaller often leaf roll, sew leaf, knot sheath, spinning netting or pierce Plant tissue feeding is caused harm.There are many important pests, such as small heart-eating peach worm, smaller apple leafrol- ler, bollworm, cabbage butterfly, pickles Moth and many Lepidoptera storehouses worm, such as Indian meal moth.In addition, famous silkworm, tussah also belong to this mesh insect.
Invention content
The purpose of the present invention is to provide a kind of insecticidal actions with both green muscardine fungus and alkaloid to be combined, to prevent squama The method of wing mesh larva.
Specific control prece is proposed according to above-mentioned purpose:
1, constituent
(1) present invention is mainly made of muscardine spore powder and biological base agent, and content is muscardine spore powder 35%- 60%, alkaloid 35%-50%, auxiliary agent 6%-15%, carrier 8%-15%;
It is preferred that:Muscardine spore powder 42%, alkaloid 39%, auxiliary agent 7%, carrier 12%;
It is preferred that:Muscardine spore powder 40%, alkaloid 40%, auxiliary agent 12%, carrier 8%;
It is preferred that:Muscardine spore powder 35%, alkaloid 46%, auxiliary agent 8%, carrier 11%;
(2) the muscardine spore powder is Metarhizium anisopliae;
(3) alkaloid described in includes:Nicotine, jervine, matrine, wilfordine, jamaicin, Sophora alopecuroide alkali, capsicum Alkali, theophylline, quinine, atropine;
(4) auxiliary agent described in is:
1. adhesive is Sodium Polyacrylate, PEG, Modified PVA, the wooden sodium, dextrin, CMC, xanthans, choose any one kind of them or several, The adhesive strength of final finished can be improved, can be liquid or solid surfactant or non-surface-active agent;
2. dispersant is phenethyl phenol polyoxyethylene sodium sulphate, AEO series, NPE, lignosulfonates are chosen any one kind of them or several Kind, so that intragranular disintegration is primary granule size by electrostatic force or steric repulsion;
3. wetting agent is oxyethylene group alkyl aryl ether, polyoxyethylene alkylether;
(5) carrier be clay, kaolin (see clay), diatomite, talcum powder it is one or more, with it is arbitrary than mix;
2, the method for being processed as pulvis
Muscardine spore powder, alkaloid, auxiliary agent is taken to be uniformly mixed ratio;Airslide disintegrating mill crushing is crossed, is added Filler is uniformly mixed;Pulverizer crushes, and is sieved by 325 mesh until reaching 98%, diameter is less than 44 microns, average grain diameter 25 Micron;
3, application method
Add water that suspension is made in prepared pulvis, is 3/square metre or more in field test lepidopterous larvae quantity When spraying application;
The beneficial effects of the present invention are:
(1) degradable in the environment, to natural enemy and beneficial organism safety, do not develop immunity to drugs;
(2) tag, stomach toxicity, infertility, food refusal, repellent, repellent rate and inhibit growth and development etc.;
Specific implementation mode
Example 1
Prevent cabbage caterpillar
It is preferred that:Muscardine spore powder 42%, alkaloid 39%, auxiliary agent 7%, carrier 12%;
(1) the muscardine spore powder is Metarhizium anisopliae;
(2) alkaloid described in includes:Nicotine, matrine;
(3) auxiliary agent described in is:
1. adhesive is Sodium Polyacrylate;2. dispersant is phenethyl phenol polyoxyethylene sodium sulphate;3. wetting agent is oxygen second Alkenylalkyl aryl ether;
(4) carrier is clay, kaolin (see clay), is mixed with arbitrary ratio;
(5) method for being processed as pulvis
Ratio is taken into muscardine spore powder, nicotine, matrine, Sodium Polyacrylate, phenethyl phenol polyoxyethylene sodium sulphate, oxygen Vinyl alkyl aryl ether is uniformly mixed;Airslide disintegrating mill crushing is crossed, clay, the kaolinite of arbitrary proportion mixing are added Soil is uniformly mixed;Pulverizer crushes, and is sieved by 325 mesh until reaching 98%, diameter is less than 44 microns, and average grain diameter 25 is micro- Rice;
(6) application method
Add water that suspension is made in prepared pulvis, is 3/square metre or more in field test lepidopterous larvae quantity When spraying application;
Example 2
Prevent bollworm
It is preferred that:Muscardine spore powder 40%, alkaloid 40%, auxiliary agent 12%, carrier 8%;
(1) the muscardine spore powder is Metarhizium anisopliae;
(2) alkaloid described in includes:Matrine, wilfordine, jamaicin;
(3) auxiliary agent described in is:
1. adhesive is dextrin;2. dispersant is lignosulfonates;
3. wetting agent is oxyethylene group alkyl aryl ether;
(4) carrier is talcum powder;
(5) method for being processed as pulvis
Ratio is taken into muscardine spore powder, matrine, wilfordine, jamaicin, dextrin, lignosulfonates, ethylene oxide Base alkyl aryl ether is uniformly mixed;Airslide disintegrating mill crushing is crossed, talcum powder is added, is uniformly mixed;Pulverizer powder It is broken, it is sieved by 325 mesh until reaching 98%, diameter is less than 44 microns, 25 microns of average grain diameter;
(6) application method
Add water that suspension is made in prepared pulvis, is 3/square metre or more in field test lepidopterous larvae quantity When spraying application;
Example 3
Prevent mythimna separata
It is preferred that:Muscardine spore powder 35%, alkaloid 46%, auxiliary agent 8%, carrier 11%;
(1) the muscardine spore powder is Metarhizium anisopliae;
(2) alkaloid described in includes:Matrine, wilfordine, jamaicin;
(3) auxiliary agent described in is:
1. adhesive is Sodium Polyacrylate;2. dispersant is phenethyl phenol polyoxyethylene sodium sulphate;3. wetting agent is polyoxy Vinyl alkyl ethers;
(4) filler is diatomite, talcum powder with arbitrary ratio mixing;
(5) method for being processed as pulvis
Ratio is taken into muscardine spore powder, matrine, wilfordine, jamaicin, Sodium Polyacrylate, phenethyl phenol polyoxy second Alkene sodium sulphate, polyoxyethylene alkylether are uniformly mixed;Airslide disintegrating mill crushing is crossed, diatomite, talcum powder is added, stirs It mixes uniformly mixed;Pulverizer crushes, and is sieved by 325 mesh until reaching 98%, and diameter is less than 44 microns, and 25 microns of average grain diameter is i.e. It can;
(6) application method
Add water that suspension is made in prepared pulvis, is 3/square metre or more in field test lepidopterous larvae quantity When spraying application;
Use interpretation of result such as following table:
As a result it shows:Prevention pair with pest, control effect unobvious after 1 day, control effect reaches best after 3 days, subtracts Pest lethal time is lacked, hence it is evident that improve the efficiency of green muscardine fungus desinsection and alkaloid desinsection.

Claims (4)

1. a kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid, which is characterized in that by muscardine spore powder and Biological base agent composition, is prepared into pulvis, and content is muscardine spore powder 35%-60%, alkaloid 35%-50%, auxiliary agent 6%-15%, carrier 8%-15%, the sum of contained raw material weight is 100% in the pulvis;The muscardine spore powder is Metarhizium anisopliae;The alkaloid includes:Nicotine, jervine, matrine, wilfordine, jamaicin, Sophora alopecuroide alkali, capsicum Alkali, theophylline, quinine, atropine;The auxiliary agent is:1. adhesive be Sodium Polyacrylate, PEG, Modified PVA, the wooden sodium, dextrin, CMC, xanthans are chosen any one kind of them or several;2. dispersant is phenethyl phenol polyoxyethylene sodium sulphate, AEO series, NPE, lignin Sulfonate is chosen any one kind of them or several;3. wetting agent is oxyethylene group alkyl aryl ether, polyoxyethylene alkylether;The carrier is Clay, kaolin, diatomite, talcum powder it is one or more, with it is arbitrary than mix;The method for being processed as pulvis is:It will Ratio takes muscardine spore powder, alkaloid, auxiliary agent to be uniformly mixed;Airslide disintegrating mill crushing is crossed, carrier is added, stirring is mixed It closes uniform;Pulverizer crushes, and is sieved by 325 mesh until reaching 98%, diameter is less than 44 microns, 25 microns of average grain diameter.
2. a kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid according to claim 1, which is characterized in that Muscardine spore powder 42%, alkaloid 39%, auxiliary agent 7%, carrier 12%.
3. a kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid according to claim 1, which is characterized in that Muscardine spore powder 40%, alkaloid 40%, auxiliary agent 12%, carrier 8%.
4. a kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid according to claim 1, which is characterized in that Muscardine spore powder 35%, alkaloid 46%, auxiliary agent 8%, carrier 11%.
CN201410414847.4A 2014-08-22 2014-08-22 A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid Expired - Fee Related CN105432669B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410414847.4A CN105432669B (en) 2014-08-22 2014-08-22 A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410414847.4A CN105432669B (en) 2014-08-22 2014-08-22 A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid

Publications (2)

Publication Number Publication Date
CN105432669A CN105432669A (en) 2016-03-30
CN105432669B true CN105432669B (en) 2018-11-13

Family

ID=55543664

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410414847.4A Expired - Fee Related CN105432669B (en) 2014-08-22 2014-08-22 A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid

Country Status (1)

Country Link
CN (1) CN105432669B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106070338B (en) * 2016-06-22 2018-10-23 北京联合大学 A kind of compounding agricultural insecticidal bactericidal composition and its application
CN108496991A (en) * 2018-03-27 2018-09-07 宁夏花杞生物科技有限公司 One kind is with the raw alkali of hardship and muscardine biological insecticides as main component
CN110759905B (en) * 2019-10-21 2021-05-11 河南科技大学 9S-acyloxy cinchonine derivative, preparation method and application thereof, and botanical pesticide
CN111802411B (en) * 2020-07-13 2021-11-30 安徽省农业科学院茶叶研究所 Pharmaceutical composition for preventing and treating plant diseases and insect pests in seedling stage of organic tea garden
CN112514917A (en) * 2020-12-03 2021-03-19 黑龙江沪生农业发展有限公司 Composite microbial pesticide and preparation method thereof
CN115053913A (en) * 2022-06-30 2022-09-16 广西壮族自治区国有钦廉林场 Compound pesticide and method for preventing and controlling underground pests

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI883094A0 (en) * 1988-06-28 1988-06-28 Kemira Oy INSECTIVE PATHOGENEIC FUERFARANDE OCH PREPARAT.
CN101773129A (en) * 2010-02-02 2010-07-14 华南农业大学 Compound insecticidal main agent of destruxins and matrine and compound insecticide
CN102265908A (en) * 2010-06-04 2011-12-07 郑州大学 Tobacco matrix composite biopesticide and preparation method thereof
CN103828842A (en) * 2014-03-04 2014-06-04 中南林业科技大学 Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof
CN103858943A (en) * 2014-03-04 2014-06-18 中南林业科技大学 Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1189086C (en) * 2002-06-03 2005-02-16 杨爱民 Pest control agent comprising multiple micro-organism
CN101438715B (en) * 2008-12-15 2011-06-22 华南农业大学 Compositional disinsection main agent and pesticide containing destruxins of Metarhizium anisopliae and buprofezin
CN101755847B (en) * 2009-11-25 2012-09-12 深圳诺普信农化股份有限公司 Metarhiziumanisopliae compound insecticide composition and use thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI883094A0 (en) * 1988-06-28 1988-06-28 Kemira Oy INSECTIVE PATHOGENEIC FUERFARANDE OCH PREPARAT.
CN101773129A (en) * 2010-02-02 2010-07-14 华南农业大学 Compound insecticidal main agent of destruxins and matrine and compound insecticide
CN102265908A (en) * 2010-06-04 2011-12-07 郑州大学 Tobacco matrix composite biopesticide and preparation method thereof
CN103828842A (en) * 2014-03-04 2014-06-04 中南林业科技大学 Sasanqua leaf extract and Metarhizium anisopliae complex insecticide and preparation method thereof
CN103858943A (en) * 2014-03-04 2014-06-18 中南林业科技大学 Complex insecticide of tung tree leaf extractive and Metarhizium anisopliae and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
印楝素农药与虫生真菌混用防治红树林鳞翅目害虫;何雪香 等;《生态科学》;20090831;第28卷(第4期);第318-323页 *
金龟子绿僵菌与农药混用室内杀虫效果研究;孙家宝 等;《北方园艺》;20070430(第04期);第220-221页 *

Also Published As

Publication number Publication date
CN105432669A (en) 2016-03-30

Similar Documents

Publication Publication Date Title
CN105432669B (en) A kind of method that green muscardine fungus is combined prevention lepidopterous larvae with alkaloid
Raghavendra et al. Malaria vector control: from past to future
RU2660673C2 (en) Pests treatment compositions and methods
Jaronski Soil ecology of the entomopathogenic ascomycetes: a critical examination of what we (think) we know
CN102626110B (en) Indoxacarb-containing complex pesticide composition
WO2010115335A1 (en) Paecilomyces cicadae biological microbial agent, preparation method therefor and use thereof in controlling plant nematodes
CN104604945B (en) A kind of Pesticidal combination containing green muscardine fungus and jervine and its application in prevention and treatment rice disease
CN101361488A (en) Disinsection compound agent capable of preventing and treating homoptera pest
CN102171327B (en) Lecanicillium muscarium strain V-5, pest extermination method using the same, and microorganism pesticide comprising the same
CN101485329B (en) Agricultural chemical composition for disinsection and use thereof
Lacey Microbial control of medically important mosquitoes in tropical climates
CN104839227A (en) Application of Bacillus amyloliquefaciens B-1619 in control of root-knot nematode disease
CN107950541A (en) A kind of garlic seed coat agent composition and preparation method thereof
CN1320857C (en) Pesticide composition containing methyl amino abamectin and bacillus thuringiensis
WO2009050482A1 (en) Composition for and method of pest control
Hall Biological control of Diaphorina citri
CN108684699A (en) A kind of food-grade mosquito eradication children's preparation and its preparation method and application
US20220340863A1 (en) Hydrogel compositions comprising protist cells
Hutapea et al. Abundance and diversity of natural enemies related to chrysanthemum aphid suppression with botanical insecticides
CN103548855B (en) Zhongshengmycin-containing bactericidal composition and application thereof
CN105010383A (en) Insecticidal composition containing dinotefuran and indoxacarb and preparation method thereof
CN107535525B (en) Biological prevention and control method for solenopsis invicta
CN105104396B (en) A kind of composition pesticide containing rosickyite azoles and amino-oligosaccharide
CN110583701A (en) Beauveria bassiana and bacterium compound pesticide for killing spodoptera frugiperda
CN104770374A (en) Bactericidal composition

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20180927

Address after: 742300 ginkgo 316 ginkgo Road, ginkgo village, Hui County, Longnan, Gansu

Applicant after: Huiyuan Luyuan Technology Development Co., Ltd.

Address before: 730600 Gansu province Baiyin Jingyuan County Ping Fort Township Chiang Tan Village lower Fort social Xia Temple 42

Applicant before: Fu Shunlin

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20181113

Termination date: 20190822

CF01 Termination of patent right due to non-payment of annual fee