CN106070338B - A kind of compounding agricultural insecticidal bactericidal composition and its application - Google Patents
A kind of compounding agricultural insecticidal bactericidal composition and its application Download PDFInfo
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- CN106070338B CN106070338B CN201610459355.6A CN201610459355A CN106070338B CN 106070338 B CN106070338 B CN 106070338B CN 201610459355 A CN201610459355 A CN 201610459355A CN 106070338 B CN106070338 B CN 106070338B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses a kind of compounding agricultural insecticidal bactericidal composition and its applications.The compounding agricultural insecticidal and bactericidal composition include green muscardine fungus, jamaicin, carrier and auxiliary agent, and the weight ratio of wherein green muscardine fungus, jamaicin, carrier and auxiliary agent is：2-20:3-20:30-70:20-60；The green muscardine fungus is green muscardine fungus conidium, and the jamaicin is monomer or mixture containing jamaicin.Green muscardine fungus and jamaicin compounding can be used for, in prevention crop pest and disease, reducing the labour cost of exclusive use, save water power for agricultural insecticidal, sterilization, environmental-friendly, can expand the desinsection of biological pesticide, fungicidal spectrum, reduce times for spraying.The present invention is free of any chemical pesticide ingredient, is easy to degrade in the environment, to people, animal, Environmental security, pathogen is not likely to produce drug resistance to it；Has the advantages that stable, efficient, low cost simultaneously.
Description
Technical field
The present invention relates to a kind of compounding agricultural insecticidal bactericidal composition and its applications, belong to technical field of pesticide.
Background technology
Green muscardine fungus (Metarhizium spp.) is a kind of important insect pathogenic fungus, using its as primary bioactivity at
Point preparation have noresidue, easy to produce, the lasting period is long, pathogenicity is strong, good application effect, to non-target organism safely etc. it is excellent
Point, has broad application prospects.There is parasitic lethal effect to a variety of agriculture and forestry injurious insects, increasingly as a kind of Biocontrol microorganism
It is concerned by people.Since the 1960s, have 50 several formulations products to be developed and registered.
But green muscardine fungus insecticide has the shortcomings that prevention is single, desinsection speed is relatively slow and the cost is relatively high, by certain in application
Limitation.
Jamaicin is primarily present in the Chinese medicines such as the coptis, Cortex Phellodendri, barberry, and such natural resources of Chinese medicinal materials is abundant.Jamaicin market
It is cheap, determine its cost advantage as disinfectant use in agriculture.As traditional antibacterials, jamaicin is to the blue sun of a variety of leather
Property and negative bacteria it is effective, clinic be mainly used for treat enteritis.Research has shown that jamaicin also has apparent anticancer, treatment glycosuria
The new functions such as disease, anti-hypertension, treatment arrhythmia cordis, can be rated as the model of old medicine newly.Jamaicin category low toxicity level (3 grades), to making
Object gray mold, powdery mildew, brown rot, late blight etc. have good preventive effect.
To improve the desinsection speed of fungus insecticide, reducing cost, the germicidal efficiency of bactericide is improved, if sterilizing, killing
Worm agent mixing application can greatly save human and material resources, reduce the access times of pesticide, but chemical bactericide Environmental compatibility
Difference, it is poor with green muscardine fungus pesticide compatibility if carbendazim, Mancozeb and thiophanate-methyl are strong to the toxic action of green muscardine fungus,
Influence the insecticidal effect of green muscardine fungus pesticide.
Inventor has found that broad-spectrum germicide jamaicin and green muscardine fungus have good compatibility, and jamaicin is to green muscardine fungus to spore
Son is sprouted without influence, and on the inhibiting rate of mycelial growth rate and sporulation quantity without influence, and the two compounds, while it is more to add synergist
Sugar, spaonin powder, vegetable protein, chitosan, green muscardine fungus, the desinsection of jamaicin, bactericidal effect significantly improve, and generate collaboration and make
With the desinsection speed and efficiency of green muscardine fungus improve, while jamaicin sterilization control effect increases.
Based on the above prior art, subsidized by " Beijing City Education Commission's city-belonged university innovation ability promotes planning item "
Complete the present invention.
Invention content
The object of the present invention is to provide a kind of compounding agricultural insecticidal bactericidal composition, have between active ingredient in the composition
There are notable synergistic effect, control effect good.
Another object of the present invention is to provide the compounding agricultural insecticidal bactericidal compositions in prevention crop pest and disease
Application in evil.
To achieve the above object, the present invention uses following technical scheme：
A kind of compounding agricultural insecticidal bactericidal composition, the composition include green muscardine fungus, jamaicin, carrier and auxiliary agent, wherein
Green muscardine fungus, jamaicin, carrier and auxiliary agent weight ratio be：2-20:3-20:30-70:20-60.
The green muscardine fungus be green muscardine fungus conidium, content be hundred million spores of 20-500/gram, the jamaicin be containing barberry
The monomer or mixture of alkali.
The total content of the green muscardine fungus and jamaicin is 5%-30%.
The carrier is in bentonite, diatomite, clay, attapulgite, kaolin, atlapulgite, mud coal, white carbon
It is one or more of.
The auxiliary agent includes synergist and dispersant.The synergist is in polysaccharide, spaonin powder, vegetable protein, chitosan
One or more.The dispersant includes spaonin powder, SOPA230, SOPA235, SOPA270, neopelex, drawing
Open powder, wetting and penetrating agent T, polysorbate60, sodium methylene bis-naphthalene sulfonate (NNO), methyl naphthalene sulfonate formaldehyde condensate (MF), wooden
Plain sodium sulfonate (calcium), sodium lignin sulfonate (calcium), alkylphenol polyoxyethylene phosphate, PVA, PEG, sodium tripolyphosphate, laundry
One or more of powder, enuatrol, polyvinyl alcohol.
It is described compounding agricultural insecticidal bactericidal composition preparation method be：The high cryptogam active compound of green muscardine fungus accurately is weighed, first
Jamaicin, auxiliary agent is added after mixing with carrier adsorption, is uniformly mixed, through being crushed to grain size in 50nm hereinafter, crossing 300 mesh
Vibrating screen, both.
The present invention also provides application of the compounding agricultural insecticidal bactericidal composition in prevention crop pest and disease, institutes
It includes aphid, scale insect, trialeurodes vaporariorum, diamondback moth etc. to state insect pest, and disease includes gray mold, powdery mildew, brown rot, late blight etc..
The advantage of the invention is that：
The compounding of green muscardine fungus of the present invention and jamaicin adds synergist, has significantly synergistic effect, control effect is good, can
To expand desinsection, the fungicidal spectrum of biological pesticide；The access times for improving the lasting period, reducing pesticide；Save human and material resources.
The function and effect of Biocontrol microorganism can be improved in the compounding of green muscardine fungus of the present invention and jamaicin, desinsection but also can kill
Bacterium obtains expected economic benefit and ecological benefits.
The present invention is free of any chemical pesticide ingredient, is easy to degrade in the environment, to people, animal, Environmental security, pathogen pair
It is not likely to produce drug resistance；Has the advantages that stable, efficient, low cost simultaneously.
Specific implementation mode
Below by way of specific embodiment, the present invention will be further described.
Embodiment 1
Green muscardine fungus MBJ6 bacterial strains, are detached by Life Sciences of Beijing University of Chemical Technology, (0.5% are added in PDA in PDAY culture mediums
Yeast extract) on cultivate, fungi preservation is in 4 DEG C.Conidia powder：Strain is connected on test tube slant, is cultivated in 25 DEG C of incubators
7d；It is transferred to again on PDAY tablets, 25 DEG C are cultivated 2 weeks, are collected after producing spore maturation spare.It is with 3.6 μ g/mL of barberry alkali concentration
A, the green muscardine fungus sterile washing spores of 20mL, if its a concentration of b (100,000 spores/mL).It is configured to train by table 1 according to isoboles
Base is supported, each group connects 5mm gray mold bacteria cakes.Blank control is only inoculated with diameter 5mm gray mold bacteria cakes, does not add jamaicin and green deadlock
Bacterium.Culture observes green muscardine fungus colony diameters for 96 hours and to ash arrhizus bacteria bacterium colony inhibiting rate, and every group is repeated 3 times.The result shows that small
Bark of a cork tree alkali sprouts muscardine spore, mycelia grows and production spore is without influence, and the two has good compatibility, even if in jamaicin
Under 1000 times of recommended densities, muscardine spore is sprouted without influence, on the inhibiting rate of mycelial growth rate and sporulation quantity without influence,
Illustrate the feasibility of the two compounding.Document once reported chemical pesticide, plant growth regulator and herbicide, such as carbendazim, Dai Sen
MnZn, thiophanate-methyl, triazolone, Acetamiprid, imidacloprid, cycocel, chlopyrifos, Rogor, gamma cyhalothrin, Avermectin
Element, metrifonate, Bordeaux mixture, pyridaben, metalaxyl, trefanocide, decis, fenvalerate have green muscardine fungus different degrees of
Toxic action can inhibit muscardine spore to sprout, mycelia growth and production spore (Zhang Yingcai, agriculture/to group, Zhang Zehua, Wang Guangjun.18
Kind chemical pesticide and green muscardine fungus Study on Compatibility.Chinese biological preventing and treating journal, 2012,28 (2)：186-191.).
The compounding result of 1 jamaicin of table and green muscardine fungus
Embodiment 2
The preparation of 13% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows：
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 100 |
| Jamaicin | 30 |
| Bentonite | 620 |
| Licorice polysaccharide | 30 |
| Pull open powder | 100 |
| Neopelex | 120 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, jamaicin 30g, licorice polysaccharide 30g, bentonite 620g, pulls open
Powder 100g, neopelex 120g.The preparation process of above-mentioned fungicide wettable powder is：Green muscardine fungus accurately is weighed,
It by the green muscardine fungus weighed up carrier bentonite adsorption, is uniformly mixed, then by the jamaicin weighed, licorice polysaccharide, detergent alkylate
Sodium sulfonate, pull open powder are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, cross 300 mesh sieve, obtain 13% (green muscardine fungus+small
Bark of a cork tree alkali) wettable powder.
Embodiment 3
The preparation of 20% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows：
| Raw material | Quality (g) |
| Green muscardine fungus (30,000,000,000 spores/g) | 100 |
| Jamaicin | 100 |
| Attapulgite | 490 |
| Rhubarb polyoses | 100 |
| Lignin calcium sulfonate | 110 |
| Calcium dodecyl benzene sulfonate | 100 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, jamaicin 100g, rhubarb polyoses 100g, sodium lignin sulfonate
110g, neopelex 100g, bentonite 490g.The preparation process of above-mentioned fungicide wettable powder is：It is accurate to claim
Take green muscardine fungus, by the green muscardine fungus weighed up with carrier attapulgite adsorb, be uniformly mixed, then by the jamaicin weighed, rhubarb polyoses,
Calcium dodecyl benzene sulfonate, lignin calcium sulfonate are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, cross 300 mesh sieve,
Obtain 20% (green muscardine fungus+jamaicin) wettable powder.
Embodiment 4
The preparation of 8% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows：
| Raw material | Quality (g) |
| Green muscardine fungus (30,000,000,000 spores/g) | 50 |
| Jamaicin | 30 |
| Spaonin powder | 50 |
| Kaolin | 590 |
| Astragalus polyose | 80 |
| Sodium tripolyphosphate | 100 |
| Neopelex | 100 |
Each raw material weighs by weight ratio, green muscardine fungus 50g, jamaicin 30g, astragalus polyose 80g, sodium tripolyphosphate 100g,
Spaonin powder 50g, neopelex 100g, kaolin 590g.The preparation process of above-mentioned fungicide wettable powder is：Essence
Really weigh green muscardine fungus, by the green muscardine fungus weighed up with carrier kaolin adsorb, be uniformly mixed, then by the jamaicin weighed, spaonin powder,
Astragalus polyose, neopelex, sodium tripolyphosphate are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, mistake
300 mesh sieve, and obtain 8% (green muscardine fungus+jamaicin) wettable powder.
Embodiment 5
The preparation of 7% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows：
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 40 |
| Jamaicin | 30 |
| Spaonin powder | 20 |
| White carbon | 600 |
| Algal polysaccharides | 80 |
| NNO | 80 |
| Calcium dodecyl benzene sulfonate | 150 |
Each raw material weighs by weight ratio, green muscardine fungus 40g, jamaicin 30g, algal polysaccharides 80g, NNO 80g, spaonin powder
20g, calcium dodecyl benzene sulfonate 150g, white carbon 600g.The preparation process of above-mentioned fungicide wettable powder is：Accurately weigh
The green muscardine fungus weighed up carrier white carbon is adsorbed, is uniformly mixed, then the jamaicin weighed, spaonin powder, seaweed is more by green muscardine fungus
Sugar, calcium dodecyl benzene sulfonate, NNO are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, cross 300 mesh sieve, obtain 7%
(green muscardine fungus+jamaicin) wettable powder.
Embodiment 6
The preparation of 25% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows：
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 150 |
| Jamaicin | 100 |
| White carbon | 350 |
| Chitosan | 120 |
| NNO | 150 |
| Calcium dodecyl benzene sulfonate | 130 |
Each raw material weighs by weight ratio, green muscardine fungus 150g, jamaicin 100g, chitosan 120g, NNO 150g, dodecane
Base benzene sulfonic acid calcium 130g, white carbon 350g.The preparation process of above-mentioned fungicide wettable powder is：Green muscardine fungus accurately is weighed, it will
The green muscardine fungus weighed up carrier white carbon adsorbs, and is uniformly mixed, then by the jamaicin weighed, chitosan, dodecyl benzene sulfonic acid
Calcium, NNO are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, cross 300 mesh sieve, obtaining 25% (green muscardine fungus+jamaicin) can
Wet powder.
Embodiment 7
The preparation of 15% (green muscardine fungus+jamaicin) wettable powder
The weight proportion of each raw material is as follows
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 100 |
| Jamaicin | 50 |
| Bentonite | 510 |
| Licorice polysaccharide | 120 |
| SOPA230 | 70 |
| Calcium dodecyl benzene sulfonate | 150 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, jamaicin 50g, licorice polysaccharide 120g, SOPA23070g, ten
Dialkyl benzene sulfonic acids calcium 150g, bentonite 510g.The preparation process of above-mentioned fungicide wettable powder is：Accurately weigh green deadlock
The green muscardine fungus weighed up carrier bentonite adsorption is uniformly mixed by bacterium, then by the jamaicin weighed, licorice polysaccharide, dodecyl
Benzene sulfonic acid calcium, SOPA230 are added, and are uniformly mixed, and are crushed 30 minutes through micronizer, cross 300 mesh sieve, obtain 15% (green muscardine fungus+
Jamaicin) wettable powder.
Embodiment 8
Field of 7% (green muscardine fungus+jamaicin) wettable powder prepared in embodiment 5 to botrytis cinerea and aphid
The test of pesticide effectiveness
It is clear green muscardine fungus+jamaicin to the field efficacy situation of graw mold of tomato, aphid, it is big in Hebei Shijiazhuang Lingshou
Canopy has carried out the medicament drug effect test of field zone of prevention graw mold of tomato.Experiment knapsack sprayer, plot experiment
It for random alignment, is repeated 3 times, per 0.3 mu of plot area, kind is chief No. two tomatoes of hat.Reagent agent dilutes 4 concentration, into
Row blade face conventional spray (occurring water membrane on blade face is advisable), May 12 spray for the first time, are spaced 10 days second spray
Medicine.Blade face conventional spray is carried out as comparison medicament prevent gray mold, Acetamiprid using 40% pyrimethanil wettable powder, 1000 times of liquid
1500 times carry out blade face conventional spray for comparison medicament and prevent eliminating aphis, and set clear water blank control.According to blank control morbidity feelings
Condition classification investigation.Gray mold uses following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for 5% or less full wafer leaf area；
3 grades：Lesion area accounts for the 6%~15% of full wafer leaf area；
5 grades：Lesion area accounts for the 16~25% of full wafer leaf area；
7 grades：Lesion area accounts for the 26~50% of full wafer leaf area；
9 grades：Lesion area accounts for 50% or more of full wafer leaf area；
According to survey data, the disease index and control effect of each processing are calculated.Disease index is calculated by formula (1), meter
It calculates result and retains 2 significant digits.
In formula, X indicates disease index；Ni indicates the sick numbers of sheets at different levels；I indicates opposite value of series；N indicates to investigate total leaf.
Control effect is calculated by formula (2).
P=100 × (CK-PT)/CK (2)
In formula, P indicates control effect (%)；CK indicates blank control disease index；PT indicates chemicals treatment disease index.
Aphid investigation method：Insect population is investigated before medicine.1d, 3d are investigated residual worm amount and (are taken for 5 points with diagonal line respectively after spray
Sample, 6 plants every, away from the residual worm amount of investigation at the 30cm of top), calculate Revision insect recluced rate and preventive effect.
Table 2 7% (green muscardine fungus+jamaicin) is to the field control effectiveness test result of graw mold of tomato
As shown in Table 2, when 500 times of 7% (green muscardine fungus+jamaicin) wettable powder dilution agent, preventive effect 83.93%, 7% is small
500 times of preventive effects of bark of a cork tree alkali wettable powder are 75.91%, hence it is evident that are less than 7% (green muscardine fungus+jamaicin) wettable powder, control
The control effect of 40% 1000 times of liquid of pyrimethanil wettable powder is 77.87%, other dilutions of green muscardine fungus and jamaicin compounding
Concentration preventive effect is above the preventive effect that jamaicin is used alone.Synthesis result, the prevention of 7% (green muscardine fungus+jamaicin) wettable powder
Graw mold of tomato effect is good, and green muscardine fungus plays synergistic effect with jamaicin compounding for the prevention of gray mold.
Table 3 7% (green muscardine fungus+jamaicin) is to the field control effectiveness test result of tomato aphid
As shown in Table 3, when 500 times of 7% (green muscardine fungus+jamaicin) wettable powder dilution agent, preventive effect is 75% or more, 7%
500 times of preventive effects of jamaicin wettable powder dilution agent are 70.95%.Synthesis result, 7% (green muscardine fungus+jamaicin) wettable powder
Any one diluted concentration control effect compared with 7% jamaicin wettable powder is high, illustrates that green muscardine fungus is multiple with jamaicin
With reaching synergistic effect for the prevention of aphid.
Claims (7)
1. a kind of compounding agricultural insecticidal bactericidal composition, it is characterised in that：The composition include green muscardine fungus, jamaicin, carrier and
Auxiliary agent, wherein green muscardine fungus, jamaicin, carrier and auxiliary agent weight ratio be：2-20:3-20:30-70:20-60；The green muscardine fungus
Be 5%-30% with the total content of jamaicin, the green muscardine fungus is green muscardine fungus conidium, content be hundred million spores of 300-500/
Gram；The auxiliary agent includes synergist and dispersant；The synergist is one or more of polysaccharide, spaonin powder, vegetable protein.
2. compounding agricultural insecticidal bactericidal composition according to claim 1, it is characterised in that：The polysaccharide is chitosan.
3. compounding agricultural insecticidal bactericidal composition according to claim 1, it is characterised in that：The jamaicin is containing barberry
The monomer or mixture of alkali.
4. compounding agricultural insecticidal bactericidal composition according to claim 1, it is characterised in that：The carrier be bentonite,
One or more of diatomite, clay, attapulgite, kaolin, atlapulgite, mud coal, white carbon.
5. compounding agricultural insecticidal bactericidal composition according to claim 1, it is characterised in that：The dispersant includes gleditsia sinensis
Powder, SOPA230, SOPA235, SOPA270, neopelex, pull open powder, wetting and penetrating agent T, polysorbate60, NNO, MF,
Sodium lignin sulfonate, calcium lignosulfonate, alkylphenol polyoxyethylene phosphate, PVA, PEG, sodium tripolyphosphate, washing powder, oil
One or more of sour sodium, polyvinyl alcohol.
6. the preparation method of compounding agricultural insecticidal bactericidal composition described in claim 1, it is characterised in that：The high cryptogam of green muscardine fungus
Active compound uses carrier adsorption first, is uniformly mixed, and jamaicin, auxiliary agent is added, and is uniformly mixed, through being crushed to grain size in 50nm hereinafter,
300 mesh vibrating screens are crossed, both.
7. application of the compounding agricultural insecticidal bactericidal composition described in claim 1 in prevention crop pest and disease, special
Sign is：The insect pest is aphid, scale insect, trialeurodes vaporariorum, one or more in diamondback moth, disease be gray mold, powdery mildew,
It is one or more in brown rot, late blight.
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| CN110447661B (en) * | 2019-08-19 | 2021-08-03 | 重庆谷百奥生物研究院有限公司 | Application of metarhizium anisopliae in preparation of medicament for preventing and treating potato late blight |
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