CN106070338A - A kind of compounding agricultural insecticidal bactericidal composition and application thereof - Google Patents
A kind of compounding agricultural insecticidal bactericidal composition and application thereof Download PDFInfo
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- CN106070338A CN106070338A CN201610459355.6A CN201610459355A CN106070338A CN 106070338 A CN106070338 A CN 106070338A CN 201610459355 A CN201610459355 A CN 201610459355A CN 106070338 A CN106070338 A CN 106070338A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses a kind of compounding agricultural insecticidal bactericidal composition and application thereof.This compounding agricultural insecticidal and bactericidal composition comprise green muscardine fungus, berberine, carrier and auxiliary agent, and wherein the weight ratio of green muscardine fungus, berberine, carrier and auxiliary agent is: 2 20: 3 20: 30 70: 20 60；Described green muscardine fungus is green muscardine fungus conidium, and described berberine is the monomer containing berberine or mixture.Green muscardine fungus and berberine compound for agricultural insecticidal, sterilization, can be used for, in preventing and treating crop pest and disease, reducing the labour cost being used alone, saving water power, environmental friendliness, can expand the parasite killing of biological pesticide, fungicidal spectrum, minimizing times for spraying.The present invention does not contains any chemical pesticide composition, is prone to degraded in the environment, and to people, animal, Environmental security, pathogen is not likely to produce Drug resistance to it；There is stable, efficient, the advantage of low cost simultaneously.
Description
Technical field
The present invention relates to a kind of compounding agricultural insecticidal bactericidal composition and application thereof, belong to technical field of pesticide.
Background technology
Green muscardine fungus (Metarhizium spp.) is the insect pathogenic fungus that a class is important, becomes with it for primary bioactivity
Point preparation have noresidue, easily productions, lasting period length, pathogenicity by force, apply effective, safely etc. excellent to non-target organism
Point, has broad application prospects.Multiple agriculture and forestry injurious insect had parasitic lethal effect, as a class Biocontrol microorganism increasingly
Paid close attention to by people.Since the sixties in 20th century, existing 50 several formulations products are developed and obtain registration.
But the shortcomings such as green muscardine fungus insecticide has that single, the parasite killing speed of preventing and treating is relatively slow and cost is the highest, by certain in application
Limit.
Berberine is primarily present in the Chinese medicines such as Rhizoma Coptidis, Cortex Phellodendri, Radix Berberidis, and this type of natural resources of Chinese medicinal materials enriches.Berberine market
Cheap, determine its cost advantage as disinfectant use in agriculture.As tradition antibacterials, berberine sun blue to multiple leather
Property and negative bacteria effective, clinic is mainly used in treating enteritis.Research proves that berberine also has the most anticancer, treatment glycosuria
The New function such as disease, resisting hypertension, treatment arrhythmia, can be rated as old medicine model newly.Berberine belongs to low toxicity level (3 grades), to work
Thing gray mold, powdery mildew, brown rot, late blight etc. have good preventive effect.
For improving the parasite killing speed of fungus insecticide, reducing cost, improve the germicidal efficiency of bactericide, if sterilizing, killing
Worm agent mixing application can be greatly saved human and material resources, the access times of minimizing pesticide, but chemical bactericide Environmental compatibility
Difference, as carbendazim, Mancozeb and thiophanate-methyl are strong to the toxic action of green muscardine fungus, poor with the green muscardine fungus pesticide compatibility,
Affect the insecticidal effect of green muscardine fungus pesticide.
Inventor finds that broad-spectrum germicide berberine and green muscardine fungus have the good compatibility, berberine to green muscardine fungus to spore
Son is sprouted without impact, and on the suppression ratio of mycelial growth rate and sporulation quantity without impact, and the two compounds, and adds synergist many simultaneously
Sugar, spaonin powder, vegetable protein, chitosan, green muscardine fungus, the parasite killing of berberine, bactericidal effect all significantly improve, and produce collaborative work
With, the parasite killing speed of green muscardine fungus and efficiency improve, and the prevention effect of berberine sterilization simultaneously increases.
Based on above prior art, " Beijing City Education Commission's city-belonged university innovation ability promotes planning item " subsidize
Complete the present invention.
Summary of the invention
It is an object of the invention to provide a kind of compounding agricultural insecticidal bactericidal composition, said composition has between effective ingredient
Having notable synergism, prevention effect is good.
Another object of the present invention is to provide described compounding agricultural insecticidal bactericidal composition in preventing and treating crop pest and disease
Application in evil.
For achieving the above object, the present invention is by the following technical solutions:
A kind of compounding agricultural insecticidal bactericidal composition, said composition comprises green muscardine fungus, berberine, carrier and auxiliary agent, wherein
The weight ratio of green muscardine fungus, berberine, carrier and auxiliary agent is: 2-20: 3-20: 30-70: 20-60.
Described green muscardine fungus is green muscardine fungus conidium, its content be 20-500 hundred million spore/gram, described berberine is containing Radix Berberidis Amurensis
The monomer of alkali or mixture.
The total content of described green muscardine fungus and berberine is 5%-30%.
Described carrier is in bentonite, kieselguhr, potter's clay, attapulgite, Kaolin, active hargil, mud coal, white carbon
One or more.
Described auxiliary agent includes synergist and dispersant.Described synergist is in polysaccharide, spaonin powder, vegetable protein, chitosan
One or more.Described dispersant includes spaonin powder, SOPA230, SOPA235, SOPA270, dodecylbenzene sodium sulfonate, draws
Open powder, wetting and penetrating agent T, polysorbate60, sodium methylene bis-naphthalene sulfonate (NNO), methyl naphthalene sulfonate formaldehyde condensate (MF), wooden
Element sodium sulfonate (calcium), sodium lignin sulfonate (calcium), alkylphenol-polyethenoxy base phosphate ester, PVA, PEG, sodium tripolyphosphate, laundry
One or more in powder, enuatrol, polyvinyl alcohol.
The preparation method of described compounding agricultural insecticidal bactericidal composition is: accurately weigh the green muscardine fungus former medicine of height cryptogam, first
With carrier adsorption, after mix homogeneously, add berberine, auxiliary agent, mix homogeneously, size-reduced to particle diameter at below 50nm, cross 300 mesh
Vibrosieve, both.
The present invention also provides for the application in preventing and treating crop pest and disease of the described compounding agricultural insecticidal bactericidal composition, institute
Stating insect pest and include aphid, scale insect, trialeurodes vaporariorum, diamondback moth etc., disease includes gray mold, powdery mildew, brown rot, late blight etc..
It is an advantage of the current invention that:
Green muscardine fungus of the present invention is compounding with berberine, adds synergist, has notable synergism, and prevention effect is good, can
To expand the parasite killing of biological pesticide, fungicidal spectrum；Improve lasting period, the access times of minimizing pesticide；Save human and material resources.
Green muscardine fungus of the present invention and the compounding action effect that can improve Biocontrol microorganism of berberine, can kill again by parasite killing
Bacterium, it is thus achieved that intended economic benefit and ecological benefits.
The present invention does not contains any chemical pesticide composition, is prone to degraded in the environment, to people, animal, Environmental security, pathogen pair
It is not likely to produce Drug resistance；There is stable, efficient, the advantage of low cost simultaneously.
Detailed description of the invention
Below by way of specific embodiment, the present invention will be further described.
Embodiment 1
Green muscardine fungus MBJ6 bacterial strain, is separated by Life Sciences of Beijing University of Chemical Technology, (adds 0.5% in PDA in PDAY culture medium
Yeast extract) upper cultivation, fungi preservation is in 4 DEG C.Spore powder: received by strain on test tube slant, cultivates in 25 DEG C of incubators
7d；It is transferred to again on PDAY flat board, cultivates 2 weeks for 25 DEG C, collect standby after producing spore maturation.With berberine concentration 3.6 μ g/mL it is
A, green muscardine fungus 20mL aseptic washing spore, if its concentration is b (100,000 spores/mL).It is configured to training by table 1 according to isoboles
Supporting base, each group connects 5mm ash arrhizus bacteria cake.Blank only inoculates diameter 5mm ash arrhizus bacteria cake, without berberine and green deadlock
Bacterium.Cultivating 96 hours and observe green muscardine fungus colony diameter and to ash arrhizus bacteria bacterium colony suppression ratio, often group is repeated 3 times.Result shows, little
Bark of a cork tree alkali is on muscardine spore sprouting, mycelial growth and product spore without impact, and the two has the good compatibility, even if at berberine
Under 1000 times of recommended densities, muscardine spore is sprouted without impact, on the suppression ratio of mycelial growth rate and sporulation quantity without impact,
The two compounding feasibility is described.Document once reported chemical pesticide, plant growth regulator and herbicide, such as carbendazim, Dai Sen
MnZn, thiophanate-methyl, triazolone, Acetamiprid, imidacloprid, chlorocholine chloride, chlopyrifos, Rogor, gamma cyhalothrin, Avermectin
Green muscardine fungus is had in various degree by element, metrifonate, Bordeaux mixture, pyridaben, metalaxyl, trefanocide, decis, fenvalerate
Toxic action, can suppress muscardine spore to sprout, mycelial growth and produce spore (Zhang Yingcai, agriculture/to group, Zhang Zehua, Wang Guangjun.18
Plant chemical pesticide and green muscardine fungus Study on Compatibility.Chinese biological preventing and treating journal, 2012,28 (2): 186-191.).
Table 1 berberine and the compounding result of green muscardine fungus
Embodiment 2
The preparation of 13% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows:
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 100 |
| Berberine | 30 |
| Bentonite | 620 |
| Angelica Polysaccharide | 30 |
| Pull open powder | 100 |
| Dodecylbenzene sodium sulfonate | 120 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, berberine 30g, Angelica Polysaccharide 30g, bentonite 620g, pulls open
Powder 100g, dodecylbenzene sodium sulfonate 120g.The preparation process of above-mentioned antibacterial wettable powder is: accurately weigh green muscardine fungus,
By the green muscardine fungus weighed up carrier bentonite adsorption, mix homogeneously, then by the berberine weighed, Angelica Polysaccharide, detergent alkylate
Sodium sulfonate, pull open powder add, mix homogeneously, through super micron mill pulverize 30 minutes, cross 300 mesh sieves, obtain 13% (green muscardine fungus+little
Bark of a cork tree alkali) wettable powder.
Embodiment 3
The preparation of 20% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows:
| Raw material | Quality (g) |
| Green muscardine fungus (30,000,000,000 spores/g) | 100 |
| Berberine | 100 |
| Attapulgite | 490 |
| Rhubarb polyoses | 100 |
| Lignin calcium sulfonate | 110 |
| Calcium dodecyl benzene sulfonate | 100 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, berberine 100g, rhubarb polyoses 100g, sodium lignin sulfonate
110g, dodecylbenzene sodium sulfonate 100g, bentonite 490g.The preparation process of above-mentioned antibacterial wettable powder is: accurately claim
Take green muscardine fungus, will weigh up green muscardine fungus carrier attapulgite absorption, mix homogeneously, then by the berberine weighed, rhubarb polyoses,
Calcium dodecyl benzene sulfonate, lignin calcium sulfonate add, mix homogeneously, pulverize 30 minutes through super micron mill, cross 300 mesh sieves,
Obtain 20% (green muscardine fungus+berberine) wettable powder.
Embodiment 4
The preparation of 8% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows:
| Raw material | Quality (g) |
| Green muscardine fungus (30,000,000,000 spores/g) | 50 |
| Berberine | 30 |
| Spaonin powder | 50 |
| Kaolin | 590 |
| Astragalus polysaccharides | 80 |
| Sodium tripolyphosphate | 100 |
| Dodecylbenzene sodium sulfonate | 100 |
Each raw material weighs by weight ratio, green muscardine fungus 50g, berberine 30g, astragalus polysaccharides 80g, sodium tripolyphosphate 100g,
Spaonin powder 50g, dodecylbenzene sodium sulfonate 100g, Kaolin 590g.The preparation process of above-mentioned antibacterial wettable powder is: essence
Really weigh green muscardine fungus, will weigh up green muscardine fungus carrier Kaolin absorption, mix homogeneously, then by the berberine weighed, spaonin powder,
Astragalus polysaccharides, dodecylbenzene sodium sulfonate, sodium tripolyphosphate add, mix homogeneously, pulverize 30 minutes through super micron mill, mistake
300 mesh sieves, obtain 8% (green muscardine fungus+berberine) wettable powder.
Embodiment 5
The preparation of 7% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows:
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 40 |
| Berberine | 30 |
| Spaonin powder | 20 |
| White carbon | 600 |
| Sargassum polysaccharides | 80 |
| NNO | 80 |
| Calcium dodecyl benzene sulfonate | 150 |
Each raw material weighs by weight ratio, green muscardine fungus 40g, berberine 30g, Sargassum polysaccharides 80g, NNO 80g, spaonin powder
20g, calcium dodecyl benzene sulfonate 150g, white carbon 600g.The preparation process of above-mentioned antibacterial wettable powder is: accurately weigh
Green muscardine fungus, the green muscardine fungus carrier white carbon that will weigh up absorption, mix homogeneously, then by many to the berberine weighed, spaonin powder, Sargassum
Sugar, calcium dodecyl benzene sulfonate, NNO add, mix homogeneously, pulverize 30 minutes through super micron mill, cross 300 mesh sieves, obtain 7%
(green muscardine fungus+berberine) wettable powder.
Embodiment 6
The preparation of 25% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows:
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 150 |
| Berberine | 100 |
| White carbon | 350 |
| Chitosan | 120 |
| NNO | 150 |
| Calcium dodecyl benzene sulfonate | 130 |
Each raw material weighs by weight ratio, green muscardine fungus 150g, berberine 100g, chitosan 120g, NNO 150g, dodecane
Base benzenesulfonic acid calcium 130g, white carbon 350g.The preparation process of above-mentioned antibacterial wettable powder is: accurately weigh green muscardine fungus, will
The green muscardine fungus weighed up carrier white carbon adsorbs, mix homogeneously, then by the berberine weighed, chitosan, DBSA
Calcium, NNO add, mix homogeneously, pulverize 30 minutes through super micron mill, cross 300 mesh sieves, and obtaining 25% (green muscardine fungus+berberine) can
WP.
Embodiment 7
The preparation of 15% (green muscardine fungus+berberine) wettable powder
The weight proportion of each raw material is as follows
| Raw material | Quality (g) |
| Green muscardine fungus (50,000,000,000 spores/g) | 100 |
| Berberine | 50 |
| Bentonite | 510 |
| Angelica Polysaccharide | 120 |
| SOPA230 | 70 |
| Calcium dodecyl benzene sulfonate | 150 |
Each raw material weighs by weight ratio, green muscardine fungus 100g, berberine 50g, Angelica Polysaccharide 120g, SOPA23070g, ten
Dialkyl benzene sulfonic acids calcium 150g, bentonite 510g.The preparation process of above-mentioned antibacterial wettable powder is: accurately weigh green deadlock
Bacterium, by the green muscardine fungus weighed up carrier bentonite adsorption, mix homogeneously, then by the berberine weighed, Angelica Polysaccharide, dodecyl
Benzenesulfonic acid calcium, SOPA230 add, mix homogeneously, pulverize 30 minutes through super micron mill, cross 300 mesh sieves, obtain 15% (green muscardine fungus+
Berberine) wettable powder.
Embodiment 8
In embodiment 5,7% (green muscardine fungus+berberine) wettable powder of preparation is to botrytis cinerea and the field of aphid
The test of pesticide effectiveness
For clear and definite green muscardine fungus+berberine to graw mold of tomato, the field efficacy situation of aphid, big in Lingshou, Shijiazhuang, Hebei
Canopy has carried out preventing and treating the drug effect test of field zone of graw mold of tomato to this medicament.Test knapsack sprayer, plot experiment
For random alignment, being repeated 3 times, every plot area 0.3 mu, kind is chief No. two Fructus Lycopersici esculenties of hat.Reagent agent 4 concentration of dilution, enter
Row blade face conventional spray (i.e. occurs on blade face that water membrane is advisable), and May 12, spray medicine for the first time, was spaced second time spray in 10 days
Medicine.Carry out blade face conventional spray with 1000 times of liquid of 40% pyrimethanil wettable powder for comparison medicament and prevent and treat gray mold, Acetamiprid
1500 times carry out blade face conventional spray for comparison medicament and prevent eliminating aphis, and set clear water blank.According to blank morbidity feelings
Condition classification is investigated.The gray mold following stage division of employing:
0 grade: anosis；
1 grade: lesion area accounts for less than the 5% of one-piece blade area；
3 grades: lesion area accounts for the 6%～15% of one-piece blade area；
5 grades: lesion area accounts for the 16～25% of one-piece blade area；
7 grades: lesion area accounts for the 26～50% of one-piece blade area；
9 grades: lesion area accounts for more than the 50% of one-piece blade area；
According to survey data, calculate disease index and the prevention effect of each process.Disease index is pressed formula (1) and is calculated, meter
Calculate result and retain 2 significant digits.
In formula, X represents disease index；Ni represents the sick number of sheets at different levels；I represents relative level numerical value；N represents the total leaf of investigation.
Prevention effect is pressed formula (2) and is calculated.
P=100 × (CK-PT)/CK (2)
In formula, P represents prevention effect (%)；CK represents blank disease index；PT represents chemicals treatment disease index.
Aphid investigation method: investigate insect population radix before medicine.After spray medicine, 1d, 3d investigate residual worm amount respectively and (take with diagonal 5
Sample, every 6 strains, at the 30cm of top, investigate residual worm amount), calculate Revision insect recluced rate and preventive effect.
The table 2 7% (green muscardine fungus+berberine) the field control effectiveness test result to graw mold of tomato
As shown in Table 2, during 7% (green muscardine fungus+berberine) wettable powder dilution agent 500 times, preventive effect is 83.93%, and 7% is little
500 times of preventive effects of bark of a cork tree alkali wettable powder are 75.91%, hence it is evident that less than 7% (green muscardine fungus+berberine) wettable powder, comparison
The prevention effect of 40% 1000 times of liquid of pyrimethanil wettable powder is 77.87%, other dilutions that green muscardine fungus is compounding with berberine
Concentration preventive effect is above being used alone the preventive effect of berberine.Synthesis result, 7% (green muscardine fungus+berberine) wettable powder is prevented and treated
Graw mold of tomato is effective, and synergism is played in the green muscardine fungus of preventing and treating to(for) gray mold compounding with berberine.
The table 3 7% (green muscardine fungus+berberine) the field control effectiveness test result to Fructus Lycopersici esculenti aphid
As shown in Table 3, during 7% (green muscardine fungus+berberine) wettable powder dilution agent 500 times, preventive effect more than 75%, 7%
500 times of preventive effects of berberine wettable powder dilution agent are 70.95%.Synthesis result, 7% (green muscardine fungus+berberine) wettable powder
It is the highest that any one concentration of dilution compares prevention effect with 7% berberine wettable powder, illustrates that green muscardine fungus is multiple with berberine
The preventing and treating in aphid of matching reaches synergism.
Claims (9)
1. a compounding agricultural insecticidal bactericidal composition, it is characterised in that: said composition comprise green muscardine fungus, berberine, carrier and
Auxiliary agent, wherein the weight ratio of green muscardine fungus, berberine, carrier and auxiliary agent is: 2-20: 3-20: 30-70: 20-60.
Compounding agricultural insecticidal bactericidal composition the most according to claim 1, it is characterised in that: described green muscardine fungus is green muscardine fungus
Conidium, its content be 20-500 hundred million spore/gram, described berberine is the monomer containing berberine or mixture.
Compounding agricultural insecticidal bactericidal composition the most according to claim 1, it is characterised in that: described green muscardine fungus and berberine
Total content be 5%-30%.
Compounding agricultural insecticidal bactericidal composition the most according to claim 1, it is characterised in that: described carrier be bentonite,
One or more in kieselguhr, potter's clay, attapulgite, Kaolin, active hargil, mud coal, white carbon.
Compounding agricultural insecticidal bactericidal composition the most according to claim 1, it is characterised in that: described auxiliary agent includes synergist
And dispersant.
Compounding agricultural insecticidal bactericidal composition the most according to claim 5, it is characterised in that: described synergist be polysaccharide,
One or more in spaonin powder, vegetable protein, chitosan.
Compounding agricultural insecticidal bactericidal composition the most according to claim 5, it is characterised in that: described dispersant includes Fructus Gleditsia
Powder, SOPA230, SOPA235, SOPA270, dodecylbenzene sodium sulfonate, pull open powder, wetting and penetrating agent T, polysorbate60, NNO, MF,
Sodium lignin sulfonate (calcium), sodium lignin sulfonate (calcium), alkylphenol-polyethenoxy base phosphate ester, PVA, PEG, sodium tripolyphosphate,
One or more in detergent, enuatrol, polyvinyl alcohol.
8. the preparation method of the compounding agricultural insecticidal bactericidal composition described in claim 1, it is characterised in that: green muscardine fungus height cryptogam
Former medicine, first with carrier adsorption, mix homogeneously, add berberine, auxiliary agent, mix homogeneously, size-reduced to particle diameter at below 50nm,
Cross 300 mesh vibrosieves, both obtained.
9. the application in preventing and treating crop pest and disease of the compounding agricultural insecticidal bactericidal composition described in claim 1, it is special
Levy and be: described insect pest is one or more in aphid, scale insect, trialeurodes vaporariorum, diamondback moth, disease be gray mold, powdery mildew,
One or more in brown rot, late blight.
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| CN110447661A (en) * | 2019-08-19 | 2019-11-15 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae in the medicament of the preparation prevention and treatment late blight of potato |
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| CN114600908A (en) * | 2022-01-25 | 2022-06-10 | 春华秋实科技集团有限公司 | Compound microorganism composition with insecticidal function and application thereof |
| CN114600908B (en) * | 2022-01-25 | 2024-01-30 | 春华秋实科技集团有限公司 | Composite microorganism composition with insecticidal function and application thereof |
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