CN101658175A - Pesticide composition and application thereof - Google Patents
Pesticide composition and application thereof Download PDFInfo
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- CN101658175A CN101658175A CN200910163768A CN200910163768A CN101658175A CN 101658175 A CN101658175 A CN 101658175A CN 200910163768 A CN200910163768 A CN 200910163768A CN 200910163768 A CN200910163768 A CN 200910163768A CN 101658175 A CN101658175 A CN 101658175A
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Abstract
The invention provides a pesticide composition and application thereof, wherein the active ingredients in the composition are amino oligosaccharin and hymexazol. The pesticide composition has the advantages that (1) the composition shows excellent synergy within a certain range of mixture ratio, thus having obviously improved sterilizing effect compared with single pesticide; (2) after the pesticide effect is improved, the composition reduces the application dosage per mu of the active ingredients, lowers the using cost and reduces pesticide residue and adverse effect for the environment; (3)the two active ingredients in the composition have different mechanisms of action, and the composition can be applied to delay or overcome the drug resistibility of pathogenic bacteria and prolong theservice life of the single pesticide; and (4) the composition has broad prevention and treatment spectrum and can be used for controlling multiple fungi and bacteria diseases of crops.
Description
Technical field
The present invention relates to a kind of composition pesticide, be used to prevent and treat the disease fungus on the agricultural, especially a kind of bactericidal composition more particularly is applicable to the composition of preventing and treating plant soil-borne diseases.
Background technology
Plant root disease control (as fusarium wilt, bacterial wilt, root rot, damping off, sudden to disease, eqpidemic disease etc.) is an insoluble important topic in the current production, reason is to solve pathogen generation, breeding and expansion in the soil, pathogen is invaded from plant root, causes root or vascular bundle to rot.Particularly in suitable temperature and humidity conditions of pathogen and continuous cropping zone for many years, disease is particularly serious.Present such disease of domestic control mainly adopts dual mode: 1, antibacterial or bactericidal assay mainly is based on tmtd, hymexazo, carbendazim or S-Ethyl ethylthio sulfonate, handles seedling stage plant mostly; 2, site competition bacteriostatic method mainly is to occupy root system of plant surrounding soil space by beneficial microbe to avoid harmful microorganism to infect from root causing disease, represent medicament to have health ground flower bud to get (cereal is glued the spore bacillus) more.These two kinds of controlling modes all have its weakness from the actual result of control, prevent and treat method for first kind certain control efficiency is arranged when plant pathogen in seedling stage just sends out, but begin when soil is bred in a large number at suitable condition pathogen, often pathogen can not be effectively killed, the pathogen infection root can not be stoped; Second kind of problem of preventing and treating method is beneficial microbe grade of fit difference under the varying environment condition, is difficult to guarantee therefore that beneficial microbe breeding survival occupies root surrounding soil space rapidly.
In view of the root disease occurrence regularity, the disease control key should adopt the integrated control strategy that improves disease resistance of plant and the early stage pathogen intrusion of control and destroy plant root.
In the control of root disease, the main medicaments such as tmtd, hymexazo, carbendazim that rely on of past.These medicaments are because long-term use has produced pesticide resistance in various degree, and control efficiency greatly reduces.A large amount of uses of medicament have simultaneously destroyed agroecological environment, and food security is also caused adverse effect.
Amino-oligosaccharide is natural chitin degrading product, a kind of bactericide with resistant effect that the microorganism belonging to genus metabolism is extracted has inhibitory action to some pathogen growth, as influences fungus spore germination, bring out the mycelia form and morph, biochemical reaction changes etc. in the born of the same parents of mycelia.Amino-oligosaccharide can strengthen its resistance and disease resistance to the immunity of disease by inducing to excite and improve plant, but strong sprout short root, promote growth and development of plants, be applicable to the control of crop root disease.The mechanism that its inducing plant produces disease resistance mainly is to excite gene expression in plants, produces the chitinase with antibacterial action. dextranase. protect element and PR albumen etc.; Have the gene expression that suppresses germ simultaneously, the Physiology and biochemistry of mycelia is morphed, growth is suppressed, the stimulating plant growth.
Amino-oligosaccharide has excellent control efficiency to the crop root disease under corresponding operation technique, but cost is higher at present, is difficult to large scale application.Itself and other active ingredient is carried out composite application, can head it off.According to the indoor and effect field practical application, judge that composite between the active ingredient is synergy or antagonism.Compound synergic is significantly filled a prescription, and can improve actual control efficiency greatly, reduces the usage amount of agricultural chemicals, thereby avoids the generation of resistance and delay the generation speed of germ resistance.
In view of this, it is significant that exploitation contains the synergistic composition of amino-oligosaccharide.
Summary of the invention
The present invention relates to a kind of multifunctional efficient plant sterilization agent prescriptions such as disease-resistant, sterilization, growth encourage that have.Purpose is to improve disease resistance of plant, suppress the plant root pathogen and promote plant growing, effectively solves puzzlement agricultural production soil-borne disease problem.
The purpose of the embodiment of the invention is to provide a kind of raising control efficiency, reduces the composition pesticide of agricultural chemicals usage amount.
Another purpose of the embodiment of the invention is to provide the application of above-mentioned composition pesticide.
The composition pesticide of the embodiment of the invention, active ingredient wherein are A (amino-oligosaccharide) and B (hymexazo).
Suo Shu hymexazo has another name called and presses down mould spirit, hydroxyisoxazole, upright withered spirit, F-319, SF-6505, belongs to systemic fungicide, is again a kind of soil disinfectant simultaneously.The damping off that rotten mildew, sickle-like bacteria etc. are caused has preventive effect preferably.This medicine combines with iron, aluminium ion in the soil, suppresses the germination of spore.Hymexazo can move by the root absorption of plant and in root system, produces two kinds of glucosides at the plant intracellular metabolite, crop is had the effect that improves physiologically active, thereby can promote plant strain growth, and the activity of the branch evil of root, the increase of root hair and root improves.
Compared with prior art, technique scheme adopt amino-oligosaccharide Yu hymexazo as active ingredient, add the composition pesticide that certain auxiliary material is mixed with certain formulation, produced following beneficial effect: (1) present composition shows fabulous synergistic effect in certain ratio range, the bactericidal effect of composition is significantly improved than single agent; (2) after drug effect improves, the mu active ingredient amount of application minimizing of composition, reduced use cost, also reduced residue of pesticide and the harmful effect of environment; (3) two kinds of active ingredient mechanism of action differences in the present composition, the application of composition can delay or overcome the pesticide resistance of pathogen, prolongs the service life of single agent; (4) present composition control spectrum is wide, multiple fungi and the bacteriosis that can prevent and treat crop.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with embodiment.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
The present patent application people finds amino-oligosaccharide energy inducing plant generation disease resistance, strong sprout to urge root, promote growth and development of plants in the chemical control research to the crop root disease, and is better to the effect of soil-borne diseases such as root rot, fusarium wilt.By further that amino-oligosaccharide and other known farm chemical ingredients is composite, find amino-oligosaccharide unexpectedly Yu after the hymexazo combination, wilt, rhizoctonia solani are all had significant synergistic effect.Discover further that again the present composition can be used for preventing and treating multiple diseases, the especially soil-borne disease on the plant, more particularly fusarium wilt, bacterial wilt, root rot, damping off, damping off, eqpidemic disease etc.
The present composition with amino-oligosaccharide Yu hymexazo is an active ingredient, amino-oligosaccharide Yu the ratio of weight and number of hymexazo at 30: 1～1: 200, more preferably 1: 1～1: 150, all the other are the auxiliary material that allows use in the agricultural chemicals, and active ingredient amino-oligosaccharide in the preparation is Yu the hymexazo cumulant is 5～90% of a composition total weight.
The present composition can adopt prior art, according to crop, the environmental condition of living in of required control, prevent and treat all kinds of factors such as method, control cost, the former medicine that will contain effective composition amino-oligosaccharide and hymexazo and suitable auxiliary material make agriculture available any formulation; Preferred formulation comprises wetting powder, missible oil, aqua, aqueous emulsion, microemulsion.
Auxiliary material in the present composition comprises that solvent, dispersant, wetting agent, emulsifier, stabilizing agent, thickener, pH regulator agent, antifreezing agent, excipient, filler and other are of value to the known substance of active ingredient stable and drug effect performance in preparation, select different auxiliary material to be mixed with corresponding formulation as required.Above-mentioned auxiliary material all is the various compositions that permission commonly used in the pesticidal preparations is used, and is not particularly limited.
The processing technology of the various preparations of the present composition adopts known method preparation, is method commonly used in the pesticidal preparations processing technology.
Product described in the invention can the finished product preparation form provide, and promptly each material mixes in the composition, and the composition of composition also can provide with single agent form, directly directly mixes in bucket or jar before the use, is diluted to required concentration then.
Embodiment 1
Former medicine (present specification Central Plains medicine refers to amino-oligosaccharide Yu hymexazo below repeats no more), various auxiliary agent and filler etc. are fully mixed, after ultrafine crusher is pulverized, be mixed with wetting powder.For example following prescription:
Amino-oligosaccharide 0.3%
Hymexazo 60%
Lauryl sodium sulfate (surfactant) 2%
White carbon black (filler) 5%
Vinylpyrrolidone (adhesive) 4%
Sodium lignin sulfonate (dispersant) 5%
Attapulgite (filler) complements to 100%
Adopt the amino oligosaccharides Su hymexazo wetting powder of present embodiment to prevent and treat the disease experiment, controlling object is the muskmelon fusarium wilt, and test site is Zhuhai, Guangdong.Dispenser for the first time period is seedling phase (about 5 centimetres of a height of seedling), gets above-mentioned wetting powder according to 2000 times of dilution sprayings and irritate root, carries out the dispenser second time after 10 at interval.7 days control efficiency are 94.57% behind the medicine for the second time.The preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 85.41%, 76.23%.
Embodiment 2
Former medicine, various auxiliary agent and filler etc. are fully mixed, after ultrafine crusher is pulverized, be mixed with wetting powder.For example following prescription:
Amino-oligosaccharide 3%
Hymexazo 0.1%
The withered powder of tea (surfactant) 5%
Polyvinyl alcohol (dispersing binder) 8%
Sodium lignin sulfonate (dispersant) 9%
Kaolin (filler) complements to 100%
Adopt the amino oligosaccharides Su hymexazo wetting powder of present embodiment to prevent and treat the disease experiment, controlling object is the fresh kidney beans root rot, and test site is Sanya, Hainan.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned wetting powder according to 1200 times of dilution sprayings and irritate root, carries out the dispenser second time after 10 at interval.7 days control efficiency are 91.35% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 82.73%, 73.28%.
Embodiment 3
Former medicine, various auxiliary agent and filler etc. are fully mixed, after ultrafine crusher is pulverized, be mixed with wetting powder.For example following prescription:
Amino-oligosaccharide 2%
Hymexazo 88%
Lauryl sodium sulfate (surfactant) 2%
White carbon black (filler) 5%
Vinylpyrrolidone (adhesive) 4%
Sodium lignin sulfonate (dispersant) 5%
Bentonite (filler) complements to 100%
Adopt the amino oligosaccharides Su hymexazo wetting powder of present embodiment to prevent and treat the disease experiment, controlling object is a rice seedling blight, and test site is Shaoyang, Hunan.Begin dispenser in their early stage, get above-mentioned wetting powder and carry out the seedbed spraying, at interval dispenser for the second time in 10 days according to 2500 times of dilutions.7 days control efficiency are 93.19% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 81.32%, 74.63%.
Embodiment 4
Following ingredients is mixed according to corresponding proportion:
Amino-oligosaccharide 2.5%
Hymexazo 2.5%
No. 500 (emulsifier) 8% of farming breast
Nongru-700 number (emulsifier) 3%
Ethanol (cosolvent) 8%
Dimethylbenzene (solvent) complements to 100%
Promptly obtain transparence 5% amino oligosaccharides Su hymexazo missible oil after the dissolving.
Adopt the amino oligosaccharides Su of present embodiment hymexazo missible oil to prevent and treat the disease experiment, controlling object is a tomato late blight, Zengcheng, test site Guangdong.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned missible oil according to 1000 times of dilution sprayings, carries out the dispenser second time after 10 at interval.7 days control efficiency are 91.27% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 83.42% and 74.25%.
Embodiment 5
Following ingredients is mixed according to corresponding proportion:
Amino-oligosaccharide 2%
Hymexazo 18%
No. 500 (emulsifier) 8% of farming breast
Nongru-700 number (emulsifier) 3%
Ethanol (cosolvent) 8%
Dimethylbenzene (solvent) complements to 100%
Promptly obtain transparence 20% amino oligosaccharides Su hymexazo missible oil after the dissolving.
Adopt the amino oligosaccharides Su of present embodiment hymexazo missible oil to prevent and treat the disease experiment, controlling object is a bacterial wilt of eggplant, Zhaoqing, test site Guangdong.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned missible oil according to 1000 times of dilution sprayings, carries out the dispenser second time after 10 at interval.10 days control efficiency are 94.78% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 85.31% and 76.49%.
Embodiment 6
Following ingredients is mixed according to corresponding proportion:
Amino-oligosaccharide 4.5%
Hymexazo 0.5%
No. 500 (emulsifier) 8% of farming breast
Nongru-700 number (emulsifier) 3%
Ethanol (cosolvent) 8%
Dimethylbenzene (solvent) complements to 100%
Promptly obtain transparence 5% amino oligosaccharides Su hymexazo missible oil after the dissolving.
Adopt the amino oligosaccharides Su of present embodiment hymexazo missible oil to prevent and treat the disease experiment, controlling object is a capsicum epidemic disease, east county, test site Hainan.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned missible oil according to 700 times of dilution sprayings and irritate root, carries out the dispenser second time after 10 at interval.10 days control efficiency are 91.05% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 82.25% and 73.41%.
Embodiment 7
Former medicine, solvent, emulsifier and cosolvent are mixed Hou Jiashui to stir.For example following prescription:
Amino-oligosaccharide 2%
Hymexazo 8%
Dimethyl sulfoxide (DMSO) (solvent) 15%
Agricultural emulsifier NO (emulsifier) 7%
Ethanol (cosolvent) 5%
Water complements to 100%
Adopt the amino oligosaccharides Su hymexazo aqua of present embodiment to prevent and treat the disease experiment, controlling object is the eggplant pythium rot, and test site is Sanya, Hainan.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned aqua according to 1000 times of dilution sprayings, carries out the dispenser second time after 10 at interval.10 days control efficiency are 93.45% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 84.17%, 75.26%.
Embodiment 8
Except that prescription, present embodiment and embodiment 7 compound methods are basic identical.It is as follows to fill a prescription:
Amino-oligosaccharide 1%
Hymexazo 15%
Dimethyl formamide (solvent) 15%
Agricultural emulsifier NO (emulsifier) 8%
Isopropyl alcohol (cosolvent) 5%
Water complements to 100%
Adopt the amino oligosaccharides Su hymexazo aqua of present embodiment to prevent and treat the disease experiment, controlling object is the sponge gourd damping off, and test site is Lingshui, Hainan.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned aqua according to 1200 times of dilution sprayings, carries out the dispenser second time after 10 at interval.10 days control efficiency are 94.36% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 85.62%, 75.47%.
Embodiment 9
Except that prescription, present embodiment and embodiment 7 compound methods are basic identical.It is as follows to fill a prescription:
Amino-oligosaccharide 0.5%
Hymexazo 15%
Dimethyl sulfoxide (DMSO) (solvent) 15%
Agricultural emulsifier NO (emulsifier) 8%
Ethanol (cosolvent) 5%
Water complements to 100%
Adopt the amino oligosaccharides Su hymexazo aqua of present embodiment to prevent and treat the disease experiment, controlling object is the balsam pear fusarium wilt, and test site is that the Guangdong height is wanted.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned aqua according to 1000 times of dilution sprayings and irritate root, carries out the dispenser second time after 10 at interval.For the second time 10 days control efficiency are 92.71% behind the medicine, 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua preventive effect be respectively 83.45%, 74.29%.
Embodiment 10
Former medicine, solvent, emulsifier added with co-emulsifier be in the same place, make and be dissolved into even oil phase.Water, antifreeze etc. is mixed, becomes the homogeneous water.Under high-speed stirred, water is joined oil phase or oil phase is joined water, form the aqueous emulsion of favorable dispersibility.It is as follows to fill a prescription:
Amino-oligosaccharide 2%
Hymexazo 18%
Polyvinyl alcohol (dispersing binder) 2%
Alkylaryl polyoxyethylene poly-oxygen propylene aether (emulsifier) 3.5%
No. 2201 (emulsifier) 5% of farming breast
Dimethyl formamide (solvent) 12%
Ethylene glycol (antifreezing agent) 4%
Organosilicon (defoamer) 0.4%
Water complements to 100%
Adopt the amino oligosaccharides Su hymexazo aqueous emulsion of present embodiment to prevent and treat the disease experiment, controlling object is a black shank, and test site is Kunming, Yunnan.Begin dispenser in their early stage, get above-mentioned aqueous emulsion, carry out the dispenser second time after 10 days at interval according to 1200 times of dilution sprayings.For the second time 10 days control efficiency are 94.38% behind the medicine, 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua preventive effect be respectively 85.71%, 76.45%.
Embodiment 11
Former medicine, solvent, emulsifier etc. are added together, make and be dissolved into even oil phase, be added to the water, stirring is mixed with microemulsion.It is as follows to fill a prescription:
Amino-oligosaccharide 1.5%
Hymexazo 3.5%
Ethanol (solvent) 4%
No. 500 (emulsifier) 2% of farming breast
Soluble starch (emulsifier) 1%
Epoxidized soybean oil (stabilizing agent) 0.5%
Castor oil polyoxyethylene polyethenoxy ether (emulsifier) 0.5%
Water complements to 100%
Adopt the amino oligosaccharides Su hymexazo microemulsion of present embodiment to prevent and treat the disease experiment, controlling object is that cucumber is sudden to sick, Wuzhou, test site Guangxi.Dispenser for the first time period is seedling phase (about 5 centimeters of a height of seedling), gets above-mentioned microemulsion according to 700 times of dilution sprayings, carries out the dispenser second time after 10 at interval.7 days control efficiency are 91.37% behind the medicine for the second time, and the preventive effect of 1000 times in contrast medicament 2% amino-oligosaccharide aqua, 800 times in 30% hymexazo aqua is respectively 82.58% and 73.61%.
Campaign shows that the control efficiency of above-mentioned prescription all is better than single agent kind, significant difference.Show that said composition has excellent synergistic effect, can reduce the formulation rate of agricultural chemicals simultaneously.
Biological activity determination
Example 1: amino-oligosaccharide is Yu the composite Toxicity Determination to the fresh kidney beans pine root fungus of hymexazo
Subjects: the fresh kidney beans pine root fungus (Fusarium solani) that picks up from the field
Test method: with reference to " the agricultural industry criteria NY/T1154.7-2006 of the People's Republic of China (PRC) " mycelial growth rate method.With fresh kidney beans pine root fungus PDA medium culture, when treating that bacterium colony has just covered with culture dish, be that the card punch of 7mm punches from the edge with internal diameter, the mycelia piece that breaks into is as inoculum.Get the soup 5mL for preparing and quantitative 75mL sterilising medium (45 ℃) respectively and mix, make 4 pastille flat boards, be mixed into contrast with equivalent sterile water and medium.After the mycelia piece is inverted and is affixed on dull and stereotyped central authorities, flat board placed in 28 ℃ of incubators cultivate.Behind the 5d, measure colony diameter, calculate and respectively handle the clean amount of growth of bacterium colony, mycelial growth inhibition rate with the right-angled intersection method.
The clean amount of growth of bacterium colony (mm)=measurement colony diameter-7
Mycelial growth inhibition rate (%)=[(the clean amount of growth of the control group bacterium colony-clean amount of growth of processed group bacterium colony)/clean amount of growth of control group bacterium colony] * 100
Mycelial growth inhibition rate is converted into probability value (y), and liquor strength (mg/L) converts logarithm value (x) to, calculates concentration EC in virulence equation and the inhibition with method of least squares
50, according to the toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.Toxicity test the results are shown in Table 1.
Composite dose co-toxicity coefficient (CTC) 〉=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Table 1 amino-oligosaccharide is Yu the composite Toxicity Determination result to the fresh kidney beans pine root fungus of hymexazo
| Handle | ??EC 50(mg/L) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Amino-oligosaccharide | ??2.35 | ??100 | ??/ | ??/ |
| Hymexazo | ??9.17 | ??25.63 | ??/ | ??/ |
| Amino-oligosaccharide 30 ∶ hymexazos 1 | ??1.78 | ??132.02 | ??97.60 | ??135.27 |
| Amino-oligosaccharide 20 ∶ hymexazos 1 | ??1.87 | ??125.67 | ??96.46 | ??130.28 |
| Amino-oligosaccharide 10 ∶ hymexazos 1 | ??1.63 | ??144.17 | ??93.24 | ??154.63 |
| Amino-oligosaccharide 1 ∶ hymexazo 1 | ??1.35 | ??174.07 | ??62.81 | ??277.13 |
| Amino-oligosaccharide 1 ∶ hymexazo 50 | ??2.57 | ??91.44 | ??27.08 | ??337.59 |
| Amino-oligosaccharide 1 ∶ hymexazo 150 | ??2.98 | ??78.86 | ??26.12 | ??301.92 |
| Amino-oligosaccharide 1 ∶ hymexazo 200 | ??4.23 | ??55.56 | ??25.99 | ??213.69 |
Measurement result shows that amino-oligosaccharide is Yu hymexazo between proportioning 30: 1～1: 200, has synergistic effect to the fresh kidney beans pine root fungus, and especially between 1: 1～1: 150, synergistic effect is more obvious, and co-toxicity coefficient is more than 270.
Example 2: amino-oligosaccharide is Yu the composite Toxicity Determination to the cucumber rhizoctonia rot bacterium of hymexazo
Subjects: the cucumber rhizoctonia rot bacterium (Rhizoctonia solani) of picking up from the field
Test method: with reference to " the agricultural industry criteria NY/T1154.7-2006 of the People's Republic of China (PRC) " mycelial growth rate method.The cucumber rhizoctonia rot bacterium is used the PDA medium culture, when treating that bacterium colony has just covered with culture dish, is that the card punch of 7mm punches from the edge with internal diameter, and the mycelia piece that breaks into is as inoculum.Get the soup 5mL for preparing and quantitative 75mL sterilising medium (45 ℃) respectively and mix, make 4 pastille flat boards, be mixed into contrast with equivalent sterile water and medium.After the mycelia piece is inverted and is connected to dull and stereotyped central authorities, flat board placed in 28 ℃ of incubators cultivate.Behind the 3d, measure colony diameter, calculate and respectively handle the clean amount of growth of bacterium colony, mycelial growth inhibition rate with the right-angled intersection method.
The clean amount of growth of bacterium colony (mm)=measurement colony diameter-7
Mycelial growth inhibition rate (%)=[(the clean amount of growth of the control group bacterium colony-clean amount of growth of processed group bacterium colony)/clean amount of growth of control group bacterium colony] * 100
The mycelial growth rate conversion is become probability value (y), and liquor strength (μ g/mL) converts logarithm value (x) to, calculates concentration EC in virulence equation and the inhibition with method of least squares
50, according to the toxicity index and the co-toxicity coefficient (CTC) of the abundant method calculating of Sun Yun medicament.Toxicity test the results are shown in Table 2.
Composite dose co-toxicity coefficient (CTC) 〉=120 shows as synergistic effect; CTC≤80 show as antagonism; 80＜CTC＜120 show as summation action.
Table 2 amino-oligosaccharide is Yu the composite Toxicity Determination result to the cucumber rhizoctonia rot bacterium of hymexazo
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Amino-oligosaccharide | ??2.61 | ??100 | ??/ | ?/ |
| Hymexazo | ??11.37 | ??22.96 | ??/ | ?/ |
| Amino-oligosaccharide 30 ∶ hymexazos 1 | ??1.97 | ??132.49 | ??97.51 | ?135.86 |
| Amino-oligosaccharide 20 ∶ hymexazos 1 | ??2.05 | ??127.32 | ??96.33 | ?132.17 |
| Amino-oligosaccharide 10 ∶ hymexazos 1 | ??2.15 | ??121.40 | ??92.99 | ?130.54 |
| Amino-oligosaccharide 1 ∶ hymexazo 1 | ??1.51 | ??172.85 | ??61.48 | ?281.16 |
| Amino-oligosaccharide 1 ∶ hymexazo 50 | ??2.93 | ??89.08 | ??24.46 | ?364.09 |
| Amino-oligosaccharide 1 ∶ hymexazo 150 | ??3.28 | ??79.57 | ??23.46 | ?339.11 |
| Amino-oligosaccharide 1 ∶ hymexazo 200 | ??4.68 | ??55.77 | ??23.34 | ?238.95 |
Measurement result shows that amino-oligosaccharide is Yu hymexazo between proportioning 30: 1～1: 200, has synergistic effect to the cucumber rhizoctonia rot bacterium, and especially between 1: 1～1: 150, synergistic effect is more obvious, and co-toxicity coefficient is more than 280.
Example 3: amino-oligosaccharide is Yu the composite Toxicity Determination to the cucurbits fusarium wilt bacterium of hymexazo
Subjects: the cucurbits fusarium wilt bacterium (Fusarium oxysporum) of picking up from the field
Test method and statistical analysis technique: with example 1
Table 3 amino-oligosaccharide is Yu the composite Toxicity Determination result to the cucurbits fusarium wilt bacterium of hymexazo
| Handle | ??EC 50(μg/mL) | ??ATI | ??TTI | Co-toxicity coefficient CTC |
| Amino-oligosaccharide | ??1.39 | ??100 | ??/ | ?/ |
| Hymexazo | ??8.27 | ??16.81 | ??/ | ?/ |
| Amino-oligosaccharide 30 ∶ hymexazos 1 | ??1.08 | ??128.70 | ??97.32 | ?132.25 |
| Amino-oligosaccharide 20 ∶ hymexazos 1 | ??1.17 | ??118.80 | ??96.04 | ??123.70 |
| Amino-oligosaccharide 10 ∶ hymexazos 1 | ??1.23 | ??113.01 | ??92.44 | ??122.25 |
| Amino-oligosaccharide 1 ∶ hymexazo 1 | ??1.03 | ??134.95 | ??58.40 | ??231.06 |
| Amino-oligosaccharide 1 ∶ hymexazo 50 | ??2.09 | ??66.51 | ??18.44 | ??360.69 |
| Amino-oligosaccharide 1 ∶ hymexazo 150 | ??2.43 | ??57.20 | ??17.36 | ??329.53 |
| Amino-oligosaccharide 1 ∶ hymexazo 200 | ??4.19 | ??33.17 | ??17.22 | ??192.63 |
Measurement result shows that amino-oligosaccharide is Yu hymexazo between proportioning 30: 1～1: 200, has obvious synergistic effect to the cucurbits fusarium wilt bacterium, and especially between 1: 1～1: 150, synergistic effect is more obvious, and co-toxicity coefficient is more than 230.
The above only is preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of being done within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1, a kind of composition pesticide is characterized in that: the composition active component is A (amino-oligosaccharide) and B (hymexazo).
2, composition pesticide according to claim 1 is characterized in that: the ratio of weight and number of composition active components A and B is 30: 1～1: 200.
3, composition pesticide according to claim 2 is characterized in that: composition active components A and B ratio of weight and number more preferably 1: 1～1: 150.
4, according to each described composition pesticide of claim 1 to 3, it is characterized in that: described combination dosage form is any one formulation that allows on the agricultural chemicals, and formulation has wetting powder, missible oil, aqua, aqueous emulsion, microemulsion preferably.
5, composition pesticide according to claim 1 is characterized in that: the accumulating weight of described composition active components A and B is 5～90% of a composition total weight.
6, according to the composition pesticide described in the claim 1, it is characterized in that: the application of composition on the control agricultural disease.
7, according to the composition pesticide described in the claim 6, it is characterized in that: the application of composition in preventing and treating soil-borne disease.
8, according to the composition pesticide described in the claim 7, it is characterized in that: the application of composition on all kinds of crop fusarium wilts of control, bacterial wilt, damping off, root rot, damping off, eqpidemic disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910163768A CN101658175A (en) | 2009-08-16 | 2009-08-16 | Pesticide composition and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910163768A CN101658175A (en) | 2009-08-16 | 2009-08-16 | Pesticide composition and application thereof |
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| Publication Number | Publication Date |
|---|---|
| CN101658175A true CN101658175A (en) | 2010-03-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910163768A Pending CN101658175A (en) | 2009-08-16 | 2009-08-16 | Pesticide composition and application thereof |
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| Country | Link |
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| CN (1) | CN101658175A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990911A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Medicine for preventing and treating ralstonia solanacearum |
| CN105145573A (en) * | 2015-08-19 | 2015-12-16 | 京博农化科技股份有限公司 | Coating composition |
| CN105613519A (en) * | 2016-04-01 | 2016-06-01 | 广西南宁益土生物科技有限责任公司 | Seed treatment agent capable of improving germination ability of seeds |
| CN105638665A (en) * | 2016-04-08 | 2016-06-08 | 广西南宁益土生物科技有限责任公司 | Composition containing lactose high-temperature treatment product and amino-oligosaccharin |
| CN107439564A (en) * | 2017-07-06 | 2017-12-08 | 王章修 | It is a kind of containing amino-oligosaccharide He the granule bactericidal composition of hymexazo |
| CN111084188A (en) * | 2019-12-30 | 2020-05-01 | 海南正业中农高科股份有限公司 | Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof |
-
2009
- 2009-08-16 CN CN200910163768A patent/CN101658175A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990911A (en) * | 2010-10-21 | 2011-03-30 | 贵州道元生物技术有限公司 | Medicine for preventing and treating ralstonia solanacearum |
| CN101990911B (en) * | 2010-10-21 | 2015-04-01 | 贵州道元生物技术有限公司 | Medicine for preventing and treating ralstonia solanacearum |
| CN105145573A (en) * | 2015-08-19 | 2015-12-16 | 京博农化科技股份有限公司 | Coating composition |
| CN105613519A (en) * | 2016-04-01 | 2016-06-01 | 广西南宁益土生物科技有限责任公司 | Seed treatment agent capable of improving germination ability of seeds |
| CN105638665A (en) * | 2016-04-08 | 2016-06-08 | 广西南宁益土生物科技有限责任公司 | Composition containing lactose high-temperature treatment product and amino-oligosaccharin |
| CN107439564A (en) * | 2017-07-06 | 2017-12-08 | 王章修 | It is a kind of containing amino-oligosaccharide He the granule bactericidal composition of hymexazo |
| CN111084188A (en) * | 2019-12-30 | 2020-05-01 | 海南正业中农高科股份有限公司 | Composition containing amino oligosaccharide, hymexazol and ethylicin and application thereof |
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Application publication date: 20100303 |