CN101317580B - Mixed insecticide of paecilomyces fumosa-roseus brown et smith and high-efficiency cypermethrin - Google Patents
Mixed insecticide of paecilomyces fumosa-roseus brown et smith and high-efficiency cypermethrin Download PDFInfo
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- CN101317580B CN101317580B CN2008100292521A CN200810029252A CN101317580B CN 101317580 B CN101317580 B CN 101317580B CN 2008100292521 A CN2008100292521 A CN 2008100292521A CN 200810029252 A CN200810029252 A CN 200810029252A CN 101317580 B CN101317580 B CN 101317580B
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Abstract
The invention discloses an insecticide compounded by paecilomyces fumosoroseus and Alpha-cypermethrin, which consists of a main agent and an auxiliary agent. According to the mass percentage, the main agent occupies 5-50% and the auxiliary agent occupies 50-95%; the main agent is the mixture of the paecilomyces fumosoroseus and the Alpha-cypermethrin with the mass proportion of 100:0.3-4; the auxiliary agent is stabilizer, assisting agent and filling material. The compounded insecticide of the invention can be processed into wettable powder, suspension agent or oil agent; according to long-term infection biological study and indoor biological testing, the paecilomyces fumosoroseus has extremely strong infection insecticidal effects on a plurality of pests, especially for the pests of piercing-sucking mouthpart. The compounded insecticide of the paecilomyces fumosoroseus and the Alpha-cypermethrin has excellent control effects on the pests such as diamondback moth, aphid, thrips, etc.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of biological insecticides, be meant a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of beta-cypermethrin especially.
Background technology
Paecilomyces fumosoroseus (Paecilomyces fumosoroseus (Wize) Brown ﹠amp; Smith) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have characteristics such as the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, can be used as the alternative control medicine of some insect that bacillus thuringiensis,Bt (Bt) is developed immunity to drugs of control, the development and use paecilomyces fumosoroseus has important economic implications.Paecilomyces fumosoroseus distributes extensively, aphid, Bemisia tabaci, diamond-back moth etc. is had good control efficiency and wide application prospect.
Beta-cypermethrin (beta-cypermethrin) is a pyrethroid insecticides, and biologically active is higher, is the efficient isomer of cypermethrin, has to tag and characteristics such as stomach poison function.Its insecticidal spectrum is wide, the speed of knocking down is fast, and insecticidal activity is applicable to pest and sanitary insect pest on the various plants such as control cotton, vegetables, fruit tree, tea tree, forest than the cypermethrin height, is mainly used in insects such as control diamond-back moth, aphid, thrips.Along with using historical prolongation, insect strengthens day by day to the pesticide resistance of beta-cypermethrin.Adopting the agriculture chemical compounding technology or mixing application technique is to overcome or delay a drug-fast important method, the compounded pesticides of development and utilization paecilomyces fumosoroseus and beta-cypermethrin, and filter out best mixed ratio, have great importance.Still find no at present the relevant paecilomyces fumosoroseus and the compounded pesticides of beta-cypermethrin.
Summary of the invention
The objective of the invention is in order to overcome the deficiencies in the prior art, a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of beta-cypermethrin are provided.
Purpose of the present invention realizes by following technical proposals:
Paecilomyces fumosoroseus and efficient cypermethrin compounded insecticide are made up of host and assistant agent, and host is 5～50% by mass percentage, and assistant agent is 50～95%; Described host is the mixture of paecilomyces fumosoroseus and beta-cypermethrin, described paecilomyces fumosoroseus: the beta-cypermethrin mass ratio is 100: 0.3～4.
Described paecilomyces fumosoroseus (Paecilomyces fumosoroseus (Wize) Brown﹠amp; Smith) bacterial strain PF01-N10, belong to hyphomycetales, paecilomyces, be located away from the Bemisia tabaci polypide of being infected in the area, Guangzhou, bacterial strain PF01-N10 preserving number: CCTCC No:M-207088, on June 27th, 2007 is in China's typical culture collection center preservation, and application number is open in the patent application of 200710031511.X.The Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form verticil mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little pale red, 3～4 * 1～2 μ m, no chlamydospore.
In order to realize the present invention better, described paecilomyces fumosoroseus: the mass ratio of beta-cypermethrin is preferably 100: 0.36～and 3.6.
Described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
Described assistant agent is stabilizing agent, auxiliary agent and filler, and by mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 9～41%.
Described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant naphthyl methane-disulfonic acid sodium (NNO); Described filler is talcum powder, potter's clay or clay.
The compounded pesticides of described paecilomyces fumosoroseus and beta-cypermethrin can be processed into formulations such as wetting powder, suspending agent or water dispersant.
The present invention compared with prior art, have following advantage and beneficial effect: the bacterial strain that (1) the present invention uses is that the Bemisia tabaci polypide of being infected from the area, GuangZhou, Guangdong Province separates acquisition, being China domestic bacterial strain, is not from external introduction, can well adapt to local natural environment.(2) the present invention has adapted to the demand that organic food is produced, and environmentally safe, noresidue help delaying pest resistance to insecticide and produce.(3) compounded pesticides of the present invention is better than only using a kind of control efficiency of medicament to the control efficiency of insect.Through long-term biological study and the indoor bioassay of infecting, paecilomyces fumosoroseus has the very strong insecticidal effect that infects to various pests especially sucking pest, and the compounded pesticides of this paecilomyces fumosoroseus and beta-cypermethrin has good control efficiency to insects such as diamond-back moth, aphid, thrips.
Embodiment
Below in conjunction with specific embodiment the present invention is explained in detail, the main thought that the present invention sets forth is the composite of paecilomyces fumosoroseus and beta-cypermethrin, embodiment helps explanation the present invention as the concrete case history of implementing of the present invention, but embodiments of the present invention are not limited to this.
Following percentage is mass percent.Bacterial strain PF01-N10 preserving number: CCTCCNo:M-207088, on June 27th, 2007 is in China's typical culture collection center preservation.
Embodiment 1:10% Paecilomyces varioti beta-cypermethrin suspending agent (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidial suspension: 20% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, and pH7.0 is in 4～8 ℃ of preservations.
B partly is a wetting powder: 4.5%EC beta-cypermethrin (commercial) 4.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 10% Paecilomyces varioti beta-cypermethrin suspending agent, paecilomyces fumosoroseus: the beta-cypermethrin mass ratio is 100: 0.9.The virulence test result is as shown in table 1.
Embodiment 2:5% Paecilomyces varioti beta-cypermethrin suspending agent (diluting 1000 times)
A partly is 10% paecilomyces fumosoroseus conidial suspension: 10% paecilomyces fumosoroseus conidial powder is dissolved in the phosphate buffer and 10% glycerine mixed liquor of 0.21M, pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 4.5%EC beta-cypermethrin 8.0%, and Tween-80 20%, sodium lignin sulfonate 6%, dispersant NNO 4% adds clay and mends to 100%.
A part and B part respectively account for 50%, directly two parts mixing are promptly obtained 5% Paecilomyces varioti beta-cypermethrin suspending agent, and described paecilomyces fumosoroseus: the beta-cypermethrin mass ratio is 100: 3.6.The virulence test result is as shown in table 1.
Embodiment 3:50% Paecilomyces varioti beta-cypermethrin wetting powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, 4.5%EC beta-cypermethrin 4%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti beta-cypermethrin wetting powder.Wherein, Paecilomyces Fumosoroseus powder: the beta-cypermethrin mass ratio is 100: 0.36.The virulence test result is as shown in table 1.
Embodiment 4:50% Paecilomyces varioti beta-cypermethrin wetting powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, the 4.5%EC beta-cypermethrin is about 3.3%, saponin 14%, Tween-80 10% adds potter's clay and mends to 100%, makes 50% Paecilomyces varioti beta-cypermethrin wetting powder.Wherein, Paecilomyces Fumosoroseus powder: the beta-cypermethrin mass ratio is 100: 0.3.
Embodiment 5:50% Paecilomyces varioti beta-cypermethrin wetting powder
Paecilomyces Fumosoroseus powder (10
10Individual/g) 50%, 4.5%EC beta-cypermethrin 8%, dodecyl sodium sulfate 14%, Tween-80 10% mixes, and adds clay then and mends to 100%, makes 50% paecilomyces fumosoroseus beta-cypermethrin wetting powder.Wherein, Paecilomyces Fumosoroseus powder: the mass ratio of beta-cypermethrin is 100: 0.72.The virulence test result is as shown in table 1.
Embodiment 6:10% Paecilomyces varioti beta-cypermethrin water dispersant (diluting 1000 times)
A partly is 20% paecilomyces fumosoroseus conidium water dispersant: 20% paecilomyces fumosoroseus conidial powder is dissolved in the glycerine mixed liquor of Tween-80 20% and 10%, and pH 7.0, in 4～8 ℃ of preservations.
B partly is a wetting powder: 4.5%EC beta-cypermethrin 8.0%, and sodium lignin sulfonate 6%, dispersant NNO 4% adds potter's clay and mends to 100%.
A part and B part respectively account for 50%, and the direct mixing of two parts is promptly obtained 10% Paecilomyces varioti beta-cypermethrin suspending agent, paecilomyces fumosoroseus: the beta-cypermethrin mass ratio is 100: 1.8.The virulence test result is as shown in table 1.
Embodiment 6: mixture is to the Toxicity Determination of diamond-back moth (Plutella xylostella)
During Toxicity Determination, (Cucumis sativus L.) makes test plant with cucumber, potted plant seedling is put into dependent insect cage, and access diamond-back moth adult, treat after it was laid eggs one day it all to be driven out of, keep 26 ± 1 ℃ temperature and 14: 10 illumination in the cage, when treating that diamond-back moth on the cucumber leaves grows to 2 instar larvaes, adopt infusion process to carry out the virulence test, 4 repetitions, 10 days effect behind the investigation medicine, the calculation correction lethality, ask the regression equation of drug concentration logarithm-lethality probability value, and carry out the F check, obtain LC then
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 paecilomyces fumosoroseus beta-cypermethrin is to the Toxicity Determination (10 of diamond-back moth
3Spore/ml, μ g/ml) (behind the medicine 10 days)
| Handle | Virulence regression equation | R 2 | LC 50And 95% confidence interval | TI |
| Paecilomyces fumosoroseus | y＝0.34x+ 3.72 | 0.9617 | 6.11，3.08-12.11 | 100.00 |
| Beta-cypermethrin (behind the medicine 3 days) | y＝1.5799x +3.0199 | 9.9881 | 17.92，16.25-19.76 | |
| Example 1: paecilomyces fumosoroseus: beta-cypermethrin (100: 3.6) | y＝0.4646x+ 3.3247 | 0.9862 | 4.04，0.34-48.19 | 151.23 |
| Example 2: paecilomyces fumosoroseus: beta-cypermethrin (100: 0.36) | y＝0.4845x+ 3.2342 | 0.9514 | 4.41，0.40-48.39 | 138.55 |
| Example 3: paecilomyces fumosoroseus: beta-cypermethrin (100: 0.72) | y＝0.4616x+ 3.3011 | 0.9708 | 4.79，0.44-52.32 | 127.56 |
| Example 4: paecilomyces fumosoroseus: beta-cypermethrin (100: 1.8) | ?y＝0.4702x+?3.2527 | 0.9807 | 5.20，0.51-53.25 | ?117.50 |
| Example 5: paecilomyces fumosoroseus: beta-cypermethrin (100: 0.9) | ?y＝0.4834x+?3.1738 | 0.9784 | 5.99，0.65-55.67 | ?102.00 |
R2: determination coefficient; TI: relative virus force index.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. the compounded pesticides of a paecilomyces fumosoroseus (Paecilomyces fumosoroseus) and beta-cypermethrin is made up of host and assistant agent, it is characterized in that host is 5～50% by mass percentage, and assistant agent is 50～95%; Described host is the mixture of paecilomyces fumosoroseus and beta-cypermethrin, described paecilomyces fumosoroseus: the beta-cypermethrin mass ratio is 100: 0.36,100: 0.72 or 100: 3.6; Described Paecilomyces Fumosoroseus strain PF01-N10 preserving number is CCTCC No:M-207088.
2. according to the compounded pesticides of described paecilomyces fumosoroseus of claim 1 and beta-cypermethrin, it is characterized in that described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or spore suspension.
3. according to the compounded pesticides of described paecilomyces fumosoroseus of claim 1 and beta-cypermethrin, it is characterized in that described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizer comprises compounded pesticides 5～40%, auxiliary agent account for compounded pesticides 2～40%, filler accounts for compounded pesticides 9～41%; Described stabilizing agent is one or more mixtures in glycerine, methyl-sulfoxide or the phosphate buffer; Described auxiliary agent is one or more mixtures among Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or the dispersant NNO; Described filler is talcum powder, potter's clay or clay.
4. according to the compounded pesticides of described paecilomyces fumosoroseus of claim 1 and beta-cypermethrin, it is characterized in that described compounded pesticides is wetting powder, suspending agent or water dispersant.
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| CN2008100292521A CN101317580B (en) | 2008-07-04 | 2008-07-04 | Mixed insecticide of paecilomyces fumosa-roseus brown et smith and high-efficiency cypermethrin |
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| CN103749546B (en) * | 2013-12-24 | 2015-12-30 | 华南农业大学 | A kind of rose dark brown Isaria and beta-cypermethrin oil-suspending agent and application thereof |
| CN103756950B (en) * | 2013-12-24 | 2016-01-20 | 华南农业大学 | Rose dark brown Isaria SCAU-IFCF02 strain liquid yeasting condition optimizing technique |
| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102297A (en) * | 1993-07-26 | 1995-05-10 | 北京市顺义县农药厂 | Efficient suspension of chloro-cyanide pyrethrin insecticide |
| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
| CN101156600A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102297A (en) * | 1993-07-26 | 1995-05-10 | 北京市顺义县农药厂 | Efficient suspension of chloro-cyanide pyrethrin insecticide |
| CN101156599A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and imidacloprid |
| CN101156600A (en) * | 2007-11-20 | 2008-04-09 | 华南农业大学 | Recompounded insecticide of paecilomyces fumosa-roseus brown et smith and aphox |
Non-Patent Citations (1)
| Title |
|---|
| 中华人民共和国农业部.农药室内生物测定试验准则 杀虫剂 第7部分：混配的联合作用测定.中华人民共和国农业行业标准NY/T 1154.7-2006.2006,(115472006),1-3. * |
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