CN103798295B - The compounded pesticides of paecilomyces fumosoroseus and Buprofezin - Google Patents
The compounded pesticides of paecilomyces fumosoroseus and Buprofezin Download PDFInfo
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- CN103798295B CN103798295B CN201310635250.8A CN201310635250A CN103798295B CN 103798295 B CN103798295 B CN 103798295B CN 201310635250 A CN201310635250 A CN 201310635250A CN 103798295 B CN103798295 B CN 103798295B
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- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 claims description 6
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- 229960001760 dimethyl sulfoxide Drugs 0.000 claims description 2
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical group O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 2
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
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- DWCZIOOZPIDHAB-UHFFFAOYSA-L methyl green Chemical compound [Cl-].[Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC(=CC=1)[N+](C)(C)C)=C1C=CC(=[N+](C)C)C=C1 DWCZIOOZPIDHAB-UHFFFAOYSA-L 0.000 description 4
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- 239000005995 Aluminium silicate Substances 0.000 description 2
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- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
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- 241001248590 Isaria Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- -1 parathiazine ketone Chemical class 0.000 description 2
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses compounded pesticides and the application thereof of a kind of paecilomyces fumosoroseus and Buprofezin.This compounded pesticides is made up of host and assistant agent, and host is 5 ~ 65% by mass percentage, and assistant agent is 35 ~ 95%; Described host is the mixture of paecilomyces fumosoroseus and Buprofezin, paecilomyces fumosoroseus: Buprofezin mass ratio is 100:1 ~ 20; Described assistant agent is stabilizing agent, auxiliary agent and filler.Compounded pesticides of the present invention can be processed into wetting powder, suspending agent or finish.Infect biological study and indoor bioassay through long-term, the insect of paecilomyces fumosoroseus to various pests especially sucking mouth parts has very strong infects insecticidal effect.Compounded pesticides of the present invention has good control efficiency to leafhopper class insects such as the aleyrodid such as Bemisia tabaci and trialeurodes vaporariorum class, tea lesser leafhoppers.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of microorganism insecticide.More specifically, a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Buprofezin is related to.
Background technology
Paecilomyces fumosoroseus (
paecilomycesfumosoroseus(Wize) Brown & Smith) be a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, have be different from existing chemical insecticide the brand-new mechanism of action, environmentally safe, noresidue feature, as preventing and treating some to the alternative protective agents of the insect that chemical insecticide and bacillus thuringiensis,Bt (Bt) develop immunity to drugs, paecilomyces fumosoroseus can be developed there is important economic implications.Paecilomyces fumosoroseus distributes extensively, has good control efficiency to insects such as Homoptera (brown planthopper, aleyrodid, coccid, aphid), Lepidoptera (silkworm moth, cloud geometrid moth), diptera (housefly), coleoptera, Hymenopteras, has a good application prospect.
Buprofezin (buprofezin) is heterocyclic Chitin synthesis inhibitor, and the newborn epidermis destroying insect is formed, and the normal growth of interference insect is grown, and causes insect dead.Action of contace poison is strong, and tool permeability and certain stomach poison function, can reduce and lay eggs and hinder egg hatching.Be applicable to prevent and treat the insect in the various plants such as paddy rice, vegetables, fruit tree, tea tree.Be mainly used in the insects such as the plant hopper of control Homoptera, leafhopper, aleyrodid and scale insect.Along with the prolongation using history, the pesticide resistance of insect parathiazine ketone has developed into a major issue, and the action effect of this chemical agent is slow simultaneously.
Adopt pesticide combination technology or mixing application technique to be overcome or delay a drug-fast important method, suppress the synthesis of insect chitin to be conducive to improving rose dark brown Isaria to the infection rate of insect, improve insecticide efficiency.The Buprofezin of rose dark brown Isaria and low dosage is used in combination be conducive to reducing Buprofezin usage amount, delay the raising of insect to its resistance level, protect ecotope, for control of insect provides new way simultaneously.
In sum, utilize paecilomyces fumosoroseus and Buprofezin to prepare compounded pesticides, and filter out best mixed ratio, will have great importance.Not yet find that there is the compounded pesticides of relevant paecilomyces fumosoroseus and Buprofezin at present.
Summary of the invention
The technical problem to be solved in the present invention overcomes the deficiencies in the prior art, provides a kind of environmental protection, pollution-free, the paecilomyces fumosoroseus of noresidue and the compounded pesticides of Buprofezin.
Another object of invention is to provide the application of described compounded pesticides.
Above-mentioned purpose of the present invention realizes by following technical scheme:
The invention provides the compounded pesticides of a kind of paecilomyces fumosoroseus and Buprofezin, be made up of host and assistant agent, host is 5 ~ 65% by mass percentage, and assistant agent is 35 ~ 95%; Described host is the mixture of paecilomyces fumosoroseus and Buprofezin, paecilomyces fumosoroseus: Buprofezin mass ratio is 100:1 ~ 20.
Described paecilomyces fumosoroseus
paecilomyces fumosoroseus(Wize) Brown & Smith bacterial strain PF01-N10, belong to hyphomycetales, paecilomyces, be located away from In Guangzhou Area by the Bemisia tabaci polypide of infecting, bacterial strain preserving number: CCTCC No:M-207088, on June 27th, 2007 is in China typical culture collection center preservation.Number of patent application is disclose in the patent application of 200710031511.X.The Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and conidiophore list is raw or be gathered into coremium, and 100 × 1.5 ~ 2 μm, wall is smooth, transparent, mostly obstructs by raw 4 ~ 6 bottles the colyliform branch forming verticil and forms.Bottle metulae portion is spherical, or plan ellipse expands, top elongated, 5.7 ~ 8 × 1 ~ 2 μm.Conidium cylindricality is to fusiformis, smooth, is clear to micro-pale red, 3 ~ 4 × 1 ~ 2 μm, without chlamydospore.
In order to realize the present invention better, the mass ratio of described paecilomyces fumosoroseus and Buprofezin is preferably paecilomyces fumosoroseus: Buprofezin=100:1.5 ~ 16.
Preferred as one, described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizing agent accounts for compounded pesticides 5 ~ 40%, auxiliary agent accounts for compounded pesticides 2 ~ 40%, filler accounts for compounded pesticides 9 ~ 41%.
Described stabilizing agent is one or more mixtures in the phosphate buffer of glycerine, methyl-sulfoxide or 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or dispersant naphthyl methane sodium disulfonate (NNO); Described filler is talcum powder, potter's clay or clay.
Compounded pesticides of the present invention can be processed into the formulations such as wetting powder, suspending agent or finish.
The present invention provides the application of described compounded pesticides in control aleyrodid class pest and leafhopper class insect simultaneously.
Most preferably, described aleyrodid class pest is Bemisia tabaci or trialeurodes vaporariorum; Described leafhopper class is tea lesser leafhopper.
The present invention has following beneficial effect:
The bacterial strain that the present invention uses obtains from In Guangzhou Area, Guangdong Province by the separation of the Bemisia tabaci polypide of infecting, and is China domestic bacterial strain, not from external introduction, can adapts to the natural environment of this locality well.
The present invention has adapted to the demand of organic foodstuff production, environmentally safe, noresidue, is conducive to delaying pest resistance to insecticide and produces.
The control efficiency of compounded pesticides of the present invention to insect is better than the control efficiency only using a kind of medicament.Biological study and indoor bioassay is infected through long-term, paecilomyces fumosoroseus has various pests especially sucking pest and very strong infects insecticidal effect, and the compounded pesticides of this paecilomyces fumosoroseus and Buprofezin has good control efficiency to leafhopper class insects such as the aleyrodid such as Bemisia tabaci and trialeurodes vaporariorum class, tea lesser leafhoppers.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, but embodiment does not limit in any form the present invention.
Described in following examples, percentage is mass percent.The strain of bacterial strain uses therefor Paecilomyces Fumosoroseus is PF01-N10, bacterial strain preserving number: CCTCC No:M-207088, and on June 27th, 2007 is in China typical culture collection center preservation.
embodiment 1 prepares 25% paecilomyces fumosoroseus buprofezin suspension agent (diluting 2000 times)
Part A is 50% paecilomyces fumosoroseus conidial suspension: 50% paecilomyces fumosoroseus conidial powder (accounting for 50% of suspension quality) be dissolved in 0.21M phosphate buffer and 10% glycerine mixed liquor in, pH 7.0, in 4 ~ 8 DEG C of preservations.
Part B is wetting powder: Buprofezin (former medicine is commercial) 8%, Tween-80 20%, sodium lignin sulfonate 6%, wetting agent LX 4%, adds potter's clay and mends to 100%.
Part A and part B respectively account for 50%, are directly mixed by two parts and namely obtain 25% Paecilomyces varioti buprofezin suspension agent, paecilomyces fumosoroseus: Buprofezin mass ratio is 100:16.Virulence test result is as shown in table 1.
embodiment 2 prepares 50% paecilomyces fumosoroseus Buprofezin finish (diluting 2000 times)
The component of finish: paecilomyces fumosoroseus conidial powder (10
10individual/g) 50%, Buprofezin (commercial) 4%, Tween-80 5%, paraffin oil 23%, methyl green 2%, adds diatomite and mends to 100%, obtained 50% Paecilomyces varioti Buprofezin finish.Wherein, paecilomyces fumosoroseus conidial powder: Buprofezin mass ratio is 100:8.Virulence test result is as shown in table 1.
embodiment 3 prepares 60% paecilomyces fumosoroseus wettable powder with buprofezin (diluting 2000 times)
The component of wetting powder: paecilomyces fumosoroseus conidial powder (10
10/ g) 60%, Buprofezin (commercial) 4%, methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, obtained 60% paecilomyces fumosoroseus wettable powder with buprofezin.Wherein, paecilomyces fumosoroseus conidial powder: the mass ratio of Buprofezin is 100:6.67.Virulence test result is as shown in table 1.
embodiment 4 prepares 50% paecilomyces fumosoroseus wettable powder with buprofezin (diluting 2000 times)
The component of wetting powder: paecilomyces fumosoroseus conidial powder (10
10/ g) 50%, Buprofezin (commercial) 2%, methyl green 2%, Tween-80 10%, sodium lignin sulfonate 6%, wetting agent LX 4%, diatomite is mended to 100%, obtained 50% rose cigarette Paecilomyces varioti wettable powder with buprofezin.Wherein, paecilomyces fumosoroseus conidial powder: the mass ratio of Buprofezin is 100:4.Virulence test result is as shown in table 1.
embodiment 5 prepares 60% paecilomyces fumosoroseus wettable powder with buprofezin (diluting 2000 times)
The component of wetting powder: paecilomyces fumosoroseus conidial powder (10
10/ g) 60%, Buprofezin (commercial) 1%, methyl green 2%, Tween-80 10%, sodium lignin sulfonate 3%, wetting agent LX 2%, kaolin is mended to 100%, obtained 60% paecilomyces fumosoroseus wettable powder with buprofezin.Wherein, paecilomyces fumosoroseus conidial powder: the mass ratio of Buprofezin is 100:1.67.Virulence test result is as shown in table 1.
embodiment 6 mixture to Bemisia tabaci (
bemisia tabaci) Toxicity Determination
During Toxicity Determination, with cucumber (
cucumis sativusl.) make test plant, Potted orchard is put into dependent insect cage, and access Bemisia tabaci adults, after it lays eggs 6h, it is all driven out of, keep the temperature of 26 ± 1 DEG C and the illumination of 14:10 in cage, when the Bemisia tabaci on cucumber leaves is developed to 2 nymph in age, adopt infusion process to carry out virulence test, repeat for 4 times, the effect of 12 days after investigation medicine, calculates corrected mortality, asks the regression equation of drug concentration logarithm-lethality probability value, and carry out F inspection, then obtain LC
50, calculate relative virus force index (TI) with reference to the abundant method of Sun Yun, its result of the test is as shown in table 1.
Table 1 paecilomyces fumosoroseus and Buprofezin to 2 age Bemisia tabaci Toxicity Determination (10
3spore/ml, μ g/ml) (after medicine 12 days).
R2: determination coefficient; TI: relative virus force index.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. a compounded pesticides for paecilomyces fumosoroseus and Buprofezin, is characterized in that, is made up of host and assistant agent, and host is 5 ~ 65% by mass percentage, and assistant agent is 35 ~ 95%; Described host is the mixture of paecilomyces fumosoroseus and Buprofezin, paecilomyces fumosoroseus: Buprofezin mass ratio is 100:1.67 ~ 16;
Described paecilomyces fumosoroseus is bacterial strain PF01-N10, bacterial strain preserving number: CCTCC No:M-207088, and on June 27th, 2007 is in China typical culture collection center preservation;
Described assistant agent is stabilizing agent, auxiliary agent and filler; By mass percentage, stabilizing agent accounts for compounded pesticides 5 ~ 40%, auxiliary agent accounts for compounded pesticides 2 ~ 40%, filler accounts for compounded pesticides 9 ~ 41%;
Described stabilizing agent is one or more mixtures in the phosphate buffer of glycerine, methyl-sulfoxide or 0.21M; Described auxiliary agent is one or more mixtures in Tween-80, washing powder, dodecyl sodium sulfate, saponin, sodium lignin sulfonate or dinaphthylmethanedisulfonic acid sodium; Described filler is talcum powder, potter's clay or clay.
2. compounded pesticides according to claim 1, it is characterized in that, described paecilomyces fumosoroseus is paecilomyces fumosoroseus conidial powder or paecilomyces fumosoroseus spore suspension.
3. compounded pesticides according to claim 1 or 2, is characterized in that, described compounded pesticides is wetting powder, suspending agent or finish.
4. the application of the arbitrary described compounded pesticides of claims 1 to 3 in control aleyrodid class pest and leafhopper class insect.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1989810A (en) * | 2005-12-30 | 2007-07-04 | 河北省农林科学院植物保护研究所 | Insecticidal composition and use thereof containing avermectin and thiazine ketone |
| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN101213965A (en) * | 2008-01-11 | 2008-07-09 | 江苏安邦电化有限公司 | Buprofezin suspension agent and preparation thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1989810A (en) * | 2005-12-30 | 2007-07-04 | 河北省农林科学院植物保护研究所 | Insecticidal composition and use thereof containing avermectin and thiazine ketone |
| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN101213965A (en) * | 2008-01-11 | 2008-07-09 | 江苏安邦电化有限公司 | Buprofezin suspension agent and preparation thereof |
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