CN101182466A - Paecilomyces Fumosoroseus strain and uses thereof - Google Patents
Paecilomyces Fumosoroseus strain and uses thereof Download PDFInfo
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- CN101182466A CN101182466A CNA200710031511XA CN200710031511A CN101182466A CN 101182466 A CN101182466 A CN 101182466A CN A200710031511X A CNA200710031511X A CN A200710031511XA CN 200710031511 A CN200710031511 A CN 200710031511A CN 101182466 A CN101182466 A CN 101182466A
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Abstract
The invention discloses a paecilomyces fumosoroseus strain. The strain is a wild strain separated from bemisia tabaci body in Guangzhou area, which is naturally infected by worm living fungus. The wild strain is re-planted on the bemisia tabaci body for the rejuvenation, so that the paecilomyces fumosoroseus strain is obtained; the purified strain is obtained through single spore isolation. The paecilomyces fumosoroseus strain is PF01-N10; the preservation number of the strain is CCTCC No: M207088; the strain is stored in China Center for Type Culture Collection on 27 June, 2007. Through a long term of infectious biology research and the indoor biology testing, the paecilomyces fumosoroseus has strong infectious and killing effects for a plurality of sucking mouth parts insects, such as the white fly and the cotton worm etc, and the chewing mouth parts.
Description
Technical field
The present invention relates to a kind of Paecilomyces Fumosoroseus (Paecilomyces fumosoroseus (Wize) Brownet Smith) bacterial strain, and the application aspect the insects such as Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid and small cabbage moth on the control brassicaceous vegetable.
Background technology
In the control of insects such as Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, small cabbage moth, adopt traditional chemical prevention and control method more, do not find to be used to prevent and treat the Paecilomyces Fumosoroseus strain of above-mentioned insect aspect preventing and treating at biological pesticide.This bacterium is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, feature with the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue can be used as the alternative control medicine of some insect that Bacillus thuringiensis (Bt) is developed immunity to drugs of control.
Summary of the invention
In order to prevent and treat insects such as Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, small cabbage moth, primary and foremost purpose of the present invention is to provide a kind of Paecilomyces Fumosoroseus strain.
Another object of the present invention is to provide the application of above-mentioned Paecilomyces Fumosoroseus strain.
Purpose of the present invention realizes by following technical proposals: a kind of Paecilomyces Fumosoroseus strain, described Paecilomyces Fumosoroseus is to separate acquisition from the area, Guangzhou by the Bemisia tabaci polypide of entomogenous fungi natural infection, the wild strain tieback is gone up rejuvenation in the Bemisia tabaci polypide obtain Paecilomyces Fumosoroseus strain, adopt monospore to separate to this bacterial strain and obtain the purifying bacterial strain.This bacterial strain is PF01-N10, the bacterial strain preserving number: CCTCC No:M207088, on June 27th, 2007 is in China's typical culture collection center preservation.Described paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown et Smith bacterial strain PF01-N10 belongs to hyphomycetales, paecilomyces.
The described Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form whorl mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little incarnadine, 3～4 * 1～2 μ m, no chlamydospore.
Above-mentioned Paecilomyces Fumosoroseus strain is prevented and treated the application in the medicines such as Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid, small cabbage moth in preparation.Paecilomyces Fumosoroseus of the present invention is effective to various pests such as the Bemisia tabaci on the control brassicaceous vegetable, Trialeurodes vaporariorum Westwood, aphid, small cabbage moths.
The present invention compared with prior art has following advantage and beneficial effect: the Bemisia tabaci polypide that this bacterial strain is infected from the area, GuangZhou, Guangdong Province separates acquisition, is China domestic bacterial strain, is not from external introduction, can adapt to local physical environment.This Paecilomyces Fumosoroseus is a kind of insect pathogenic fungus, as a kind of living body biological agricultural chemicals, has the feature of the brand-new mechanism of action that is different from existing chemical insecticide, environmentally safe, noresidue, has adapted to the requirement that Organic food is produced.Paecilomyces Fumosoroseus of the present invention is effective to various pests such as the Bemisia tabaci on the control brassicaceous vegetable, Trialeurodes vaporariorum Westwood, aphid, small cabbage moths.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Embodiment 1
1.1 material source
On the geographic vegetables cucumber in Guangzhou, collect a kind of by the Bemisia tabaci Bemisiatabaci worm corpse of worm fungal infection.
Potato dextrose agar (PDA): the 200g potato boiled get juice, in the juice of being got, add 20g glucose+17～20g agar powder then, add entry to make cumulative volume be 1000ml and boil.The pot sterilization after high pressure goes out (121 ℃, 30min).
Aseptic technique: all vessel and apparatus must go out through high pressure, and (121 ℃, 30min), operations such as inoculation are all carried out in Bechtop in the pot sterilization.
Culture condition: place 25 ℃ of illumination (14L:10D) thermostat container to cultivate, treat that bacterium colony forms after, transfer to the PDA inclined-plane, change 4 ℃ of freezer storages again over to.
1.2 the separation of pathogenic bacteria, evaluation
(1) isolation and purification of pathogenic bacteria
(1) separates
Bacterial isolate bacterium from the tobacco powder mite corpse sample of adopting back that is infected by entomogenous fungi.Utilize 5% chlorine bleach liquor that sample is carried out surface sterilization, the sample after the sterilization washs in aqua sterilisa 3 times, and puts into the PDA flat board, is inverted in 25 ℃ of thermostat containers and cultivates, treat that bacterium colony forms after, transfer to the PDA inclined-plane, change 4 ℃ of freezer storages again over to.
(2) rejuvenation
The bacterial strain that is separated to was cultivated 7 days on the PDA flat board, and the sterilized water that adds 0.3% tween-80 behind the bacterium colony to be formed is collected spore, and spore suspension was stirred on magnetic stirring apparatus 20 minutes, will be diluted to 1 * 10 with small-sized hand-operated atomizer
7The spore suspension of spore/ml is sprayed on the cucumber leaves that has Bemisia tabaci equably, is incubated more than 90% 24 hours.The infected Bemisia tabaci of picking is separated to the A strain isolated by foregoing method after 10 days.
(3) purifying
The A strain isolated is cultivated 10d respectively on the PDA flat board, behind the rose dark brown spore to be formed, the picking conidium makes 1 * 10
3The spore suspension of spore/ml, suspension is dripped on the slide glass that is placed with cover glass, under biomicroscope, observe, will have only a conidial slide to insert on the PDA substratum in the drop, be placed in the incubator and cultivate, obtain B, C, D, E, F, G, H, I totally 8 strain isolateds.
(4) evaluation of pathogenic bacteria
Form according to cultivation proterties, mycelia, conidium and the product spore device of pathogenic bacteria is carried out preliminary evaluation.
1, colonial morphology is described
8 strain isolateds that obtain are inoculated on the PDA flat board, under 25 ℃ of conditions, cultivate.Can see the mycelia that grows white in 2-3 days, bacterium colony long wool shape, white, middle part protuberance, 5d produces the Powdered spore of rose dark brown.The bacterium colony back side is colourless or yellow.
2, mycelia and spore shape are described
The conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, and the colyliform branch of forming whorl by living 4～6 bottles stalk is formed mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality to fusiformis, smooth, be clear to little incarnadine, 3～4 * 1～2 μ m, no chlamydospore.
(2) screening of paecilomyces fumosoroseus strain isolated
Entomogenous fungi has diversity at aspects such as heredity, ecology and biology, has the parasexuality phenomenon again, and the different strains of fungi of the same race is to the virulence significant difference of target pest, bacterial strain difference, its LD
50, LT
50Can differ several times to tens times.The screening of high yield and high quality bacterial strain and acquisition are the primary prerequisites that obtains better prevention effect.4 indexs that bacterial strain screening is commonly used are respectively virulence, sporulation quantity, spore germination rate, colony growth speed.The present invention is a foundation with these indexs, the dominant strain of screening paecilomyces fumosoroseus.
(1) processing of strains tested
Paecilomyces fumosoroseus B, the C that purified back obtains, D, E, F, G, H, I be totally 8 strain isolateds, cultivates in 25 ± 0.5 ℃ thermostat container (14L:10D) at the PDA flat board.
(2) for examination insect and host plant
Bemisia tabaci, the Bemisia tabaci adults with raising in the solarium on cucumber is inoculated on the cucumber seedling of no worm, behind the 12h of laying eggs, removes adult, and cucumber seedling is placed 25 ± 0.5 ℃ illumination box, treats that the Bemisia tabaci growth is stand-by during nymph to 2 ages.Cucumber Cucumis sativus L., kind is ten thousand lucky green cucumbers, available from academy of agricultural sciences, Guangdong Province Vegetable Research Institute, test seedling all adopts 2～3 potted plant seedlings that launch leaf.
(3) mensuration of colony growth speed and sporulation quantity
8 strain isolateds are mixed with 1 * 10 respectively
7The conidium suspension of spore/ml, get the 1ml suspension respectively and splash on the PDA substratum, smoothen, treat that it is the fresh bacterium colony of punch tool of 13mm that 1～2d grows behind the mycelia with diameter with the triangle glass stick, be inoculated on the PDA flat board, place incubator to cultivate (L: D=14: 10) then.Each bacterial strain repeats for 5 times.10d measures colony diameter and collects conidium and measure sporulation quantity with the blood counting chamber numeration.Concrete operations are as follows: measure colony diameter with ruler, and be that the sterilization punch tool of 5mm is got the uniform bacterium piece of mycelial growth in culture dish with diameter, put into then and be added with 20ml0.3% tween-80 sterilized water, on magnetic stirring apparatus, stirred 20 minutes, spore is fully disperseed, make spore suspension, measure sporulation quantity with the blood cell counting plate numeration.
The colony growth speed and the sporulation quantity of 8 strain isolateds see Table 1, colony growth speed is except that C, F, I and B, G, H significant difference, difference is not remarkable between remaining strain isolated, the colony growth speed maximum of H strain isolated wherein, the colony growth speed minimum of C strain isolated.Behind the growth 10d, except that the sporulation quantity significant difference of strain isolated C and B, D, G and I, the sporulation quantity difference between other strain isolated is not remarkable, and wherein the sporulation quantity of G strain isolated is the highest.
8 strain isolated growth velocitys of table 1 paecilomyces fumosoroseus and sporulation quantity (10d)
| Strain isolated | Colony growth speed M ± SE (mm) | Sporulation quantity (conidium/mL) |
| B C D E F G H 1 | 16.32±1.19a 14.12±1.06b 15.60±1.21ab 15.40±0.78ab 14.34±1.33b 16.98±1.13a 17.26±1.34a 14.83±2.05b | 2.96×10 9a 1.45×10 9b 2.71×10 9a 2.21×10 9ab 2.02×10 9ab 3.04×10 9a 2.98×10 9a 3.01×10 9a |
Annotate: represent difference not remarkable (DMRT method) with alphabetical identical person behind the column of figure in the table.
(4) mensuration of spore germination rate
After 8 strain isolateds are cultivated 10d respectively, collect spore and make suspension, test with slide glass sprouting method with sterilized water.Spore suspension is dropped on the aseptic slide glass, places the bottom to be covered with in the culture dish of filter paper, in ware, drip 3～4 sterilized waters preserve moisture (100, RH), cultivate microscopy behind the 24h.Each handles 3 repetitions.
In 8 strain isolateds, the conidia germination rate significant difference (seeing Table 2) between C, D strain isolated and other strain isolated.Wherein the average germination rate of the spore of G strain isolated is the highest, and the spore germination rate of D strain isolated is minimum.
16 strain isolated gonidiums of table 2 paecilomyces fumosoroseus spore germination rate (24h)
| Strain isolated | Spore | Germination rate (%) M ± SE |
| B C D E F G H I | 563 683 760 673 671 843 673 791 | 84.55+1.32a 71.43±2.19b 46.984±5.12c 91.024±1.04a 90.15±1.13a 89.974±1.03a 82.72±2.09a 86.884±1.11a |
Annotate: represent difference not remarkable (DMRT method) with alphabetical identical person behind the column of figure in the table.
(5) strain isolated is to the mensuration of the virulence of Bemisia tabaci nymph
Behind 8 strain isolateds cultivation lOd, collect spore with sterilized water, being mixed with concentration is 10
7The spore suspension of conidium/mL.To have 2 age the Bemisia tabaci nymph cucumber leaves immerse in the suspension, contrast is immersed in the sterilized water, takes out behind the 20s, dries naturally.Whenever be treated to 3～4 leaves, 50～100 nymphs of every leaf, 3 repetitions.Take the blade of handling, insert in the colored mud that fills sterilized water, place in the transparent crisper.Place 25+0.5 ℃ illumination box (L: D=14: 10) again.Every day, microscopy and record infected mortality ratio, observed 7d continuously.Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
The virulence result of study shows that the infection rate of all strain isolateds is significant difference compared with the control.8 strain isolateds begin to show at 4d and infect symptom, can find out that from the infection rate of 4d E, G strain isolated are significantly higher than B, C, D, F, H, 1 strain isolated, the infection rate significant difference of D strain isolated and other strain isolated during 7d.From in general, the virulence of strain isolated G is stronger, average out to 95.22% (7d), and the infection rate of D strain isolated is lower, is 61.04% (7d) (table 3).
The virulence of 8 strain isolateds of table 3 paecilomyces fumosoroseus
| Strain isolated | Infection rate (%) M ± SE (4d) | Infection rate (%) M ± SE (7d) |
| B C D E F G H I CK | 66.80±6.15c 76.64±5.04b 56.07±6.73c 88.47±2.29a 71.81±2.32b 90.80±1.83a 78.85±6.02b 79.03±3.62b 5.14±2.06d | 78.23±7.14b 90.57±5.12a 61.04±8.13c 92.14±4.85a 89.76±3.44ab 95.22±0.52a 90.01±2.19a 87.79±1.37ab 8.12±3.44d |
Annotate: represent difference not remarkable (DMRT method) with alphabetical identical person behind the column of figure in the table.
(6) screening of paecilomyces fumosoroseus strain isolated
When the good strain isolated of screening, pathogenic and sporulation quantity is important reference index, and spore germination rate and colony growth speed are taken second place.In the present invention, colony growth speed is except that C, F, I and B, G, H significant difference, and difference is not remarkable between remaining strain isolated.Conidia germination rate difference is not remarkable between other strain isolated except that C, D.From sporulation quantity and pathogenic on, strain isolated G and H are comparatively good, have characteristics (table 1, table 3) such as pathogenic strong, sporulation quantity height.The comprehensive relatively factor of each side, the G strain isolated is an optimum strain, with its called after PF01-N10 bacterial strain, be accredited as paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown et Smith through professor Liu Aiying of Guizhou University, its bacterial strain preserving number: CCTCC No:M207088, on June 27th, 2007 is in China's typical culture collection center preservation.This paecilomyces fumosoroseus Paecilomyces fumosoroseus (Wize) Brown et Smith bacterial strain PF01-N10 belongs to hyphomycetales, paecilomyces.This Paecilomyces Fumosoroseus back side that falls is colourless or yellow, and the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth, and is transparent, forms by giving birth to the colyliform branch that 4～6 bottles stalks form whorl mostly.Bottle metulae portion sphere, or intend ellipse and expand top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little incarnadine, 3～4 * 1～2 μ m, no chlamydospore.
Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
Embodiment 2 paecilomyces fumosoroseus PF01-N10 bacterial strains are measured the virulence of Bemisia tabaci
Biological assay is to detect entomogenous fungi to one of the deadly degree of target pest and effective means of fatality rate, can provide important reference frame for the biological control potentiality of this fungi of comprehensive evaluation.The present invention measures the virulence of Bemisia tabaci with regard to paecilomyces fumosoroseus PF01-N10 bacterial strain, to filter out optimum concn used when preventing and treating.
For trying insect and host plant: Bemisia tabaci, the Bemisia tabaci adults with raising in the greenhouse on cucumber is inoculated on the cucumber seedling of no worm, lay eggs behind the 12h, remove adult, cucumber seedling is placed 25 ± 0.5 ℃ illumination box, treat that Bemisia tabaci grows to 2 ages stand-by during nymph.Cucumber Cucumis sativus L., kind is ten thousand lucky green cucumbers, available from academy of agricultural sciences, Guangdong Province Vegetable Research Institute, test seedling all adopts 2～3 potted plant seedlings that launch leaf.
(1) processing of strains tested
The paecilomyces fumosoroseus PF01-N10 bacterial strain that purified back obtains, adopt the PDA flat board, in 25 ± 0.5 ℃ thermostat containers (14L: cultivated 10 days 10D), the sterilized water that adds 0.3% tween-80 is collected spore, and spore suspension was stirred on magnetic stirring apparatus 20 minutes, treat sporocyst break up even after, filter decon with hospital gauze, obtain spore suspension, behind the definite spore concentration of blood cell counting plate numeration, be made into 1 * 10 again
3, 1 * 10
4, 1 * 10
5, 1 * 10
6, 1 * 10
75 concentration gradients of spore/ml are stand-by.
(2) strain isolated is measured the virulence of Bemisia tabaci nymph
The cucumber leaves that will have Bemisia tabaci nymph in 2 age immerses in the spore suspension of 6 gradients, and contrast is immersed and contained in the sterilized water of 0.3% tween-80, takes out behind the 20s, dries naturally.Whenever be treated to 3～4 leaves, 50～100 nymphs of every leaf, 3 repetitions.The blade of handling is positioned over (L: D=14: 10) in 25 ± 0.5 ℃ the illumination box together with cucumber seedling.Every day, microscopy and record infected mortality ratio, observed 14d continuously.Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
(3) the paecilomyces fumosoroseus strain is to the Bemisia tabaci cumulative mortality in 2 ages
Postvaccinal the 2nd～3d, the nymph of each treatment zone begins to show disease symptom, body colour as Bemisia tabaci gradually becomes white by transparent, can be observed subsequently has the mycelia of a small amount of white to grow on the polypide, linen spore appears in the polypide surface after several days, along with the intensification of spore color, Bemisia tabaci death.Table 4 is that paecilomyces fumosoroseus handles the cumulative mortality of back nymph 7d in 2 age, 14d, and the result shows that the mortality ratio along with 2 ages of increase Bemisia tabaci of concentration also increases, and along with the increase of time, the mortality ratio in 2 ages of Bemisia tabaci also increases under the same concentration.Wherein 1 * 10
6With 1 * 10
7Treatment zone, the cumulative mortality difference of Bemisia tabaci is not remarkable.
The paecilomyces fumosoroseus of table 4 different concns is to the cumulative mortality of Bemisia tabaci nymph in 2 age
| Concentration | 7d | 14d |
| 1×10 3 1×10 4 1×10 5 1×10 6 1×10 7 CK | 0.27±1.03c 0.37±0.98bc 0.48±1.64b 0.57±1.45ab 0.69±2.01a 0.06±0.76c | 0.44±1.33c 0.54±1.15c 0.73±1.38b 0.81±1.86ab 0.93±2.15a 0.09±0.83d |
Annotate: represent difference not remarkable (DMRT method) with alphabetical identical person behind the column of figure in the table.
Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
Embodiment 3 paecilomyces fumosoroseus PF01-N10 bacterial strains are measured the virulence of diamondback moth larvae
For trying insect and host plant: small cabbage moth, the small cabbage moth adult with raising in the solarium on Plantula Brassicae chinensis is inoculated on the Plantula Brassicae chinensis seedling of no worm, lay eggs behind the 12h, remove adult, the Plantula Brassicae chinensis seedling is placed 25 ± 0.5 ℃ illumination box, treat when small cabbage moth grows to 2 instar larvaes stand-by.Plantula Brassicae chinensis Brassicacampestris L.ssp.chinensis, available from academy of agricultural sciences, Guangdong Province Vegetable Research Institute, test seedling all adopts 6～8 potted plant seedlings that launch leaf.
(1) processing of strains tested
The paecilomyces fumosoroseus PF01-N10 bacterial strain that purified back obtains, in 25 ± 0.5 ℃ thermostat container (14L:10D), cultivating 10 days on the PDA flat board, the sterilized water that adds 0.3% tween-80 is collected spore, spore suspension was stirred on magnetic stirring apparatus 20 minutes, after treating that sporocyst is broken up evenly, filter decon, obtain spore suspension with hospital gauze, behind the definite spore concentration of blood cell counting plate numeration, be made into 1 * 10 again
3, 1 * 10
4, 1 * 10
5, 1 * 10
6, 1 * 10
75 concentration gradients of spore/ml are stand-by.
(2) strain isolated is measured the virulence of diamondback moth larvae
The Plantula Brassicae chinensis blade that will have small cabbage moth 2 instar larvaes immerses in the spore suspension of 5 gradients, and contrast is immersed in the sterilized water, takes out behind the 20s, dries naturally.Whenever be treated to 3～4 leaves, 10 nymphs of every leaf, 3 repetitions.The blade of handling is put into the pallet (25cm * 35cm), and be positioned over (L: D=14: 10) in 25 ± 0.5 ℃ the illumination box of sterilization.Every day, microscopy and record infected mortality ratio, and changed fresh cabbage leaf, observed 10d continuously.Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
(3) the Paecilomyces Fumosoroseus strain is to the small cabbage moth cumulative mortality in 2 ages
Postvaccinal the 2nd～3d, the larva of each treatment zone begins to show disease symptom, is slow in action, loses the appetite as polypide, can be observed subsequently has the mycelia of a small amount of white to grow on the polypide, linen spore appears in the polypide surface after several days, along with the intensification of spore color, and Bemisia tabaci death.Table 4 is handled the cumulative mortality of 7d, 14d behind 2 instar larvaes for paecilomyces fumosoroseus, and the result shows the increase along with concentration, and the mortality ratio in 2 ages of small cabbage moth also increases, and along with the increase of time, the mortality ratio in 2 ages of small cabbage moth also increases under the same concentration.Wherein 1 * 10
6With 1 * 10
7Treatment zone, the cumulative mortality difference of small cabbage moth is not remarkable.
The paecilomyces fumosoroseus of table 4 different concns is to the cumulative mortality of small cabbage moth 2 instar larvaes
| Concentration | 5d | 10d |
| 1×10 3 1×10 4 1×10 5 1×10 6 1×10 7 CK | 0.29±0.93c 0.36±0.75bc 0.45±1.04b 0.65±1.52ab 0.81±2.32a 0.05±0.47c | 0.46±1.13c 0.57±1.11c 0.76±1.13b 0.86±1.62ab 0.95±2.07a 0.09±0.78d |
Annotate: represent difference not remarkable (DMRT method) with alphabetical identical person behind the column of figure in the table.
Above-mentioned all testing datas are all finished dealing with in data processing software SAS system.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. Paecilomyces Fumosoroseus strain, it is characterized in that: described bacterial strain is Paecilomyces Fumosoroseus PF01-N10, the bacterial strain preserving number: CCTCC No:M207088, on June 27th, 2007 is in China's typical culture collection center preservation.
2. a kind of Paecilomyces Fumosoroseus strain according to claim 1 is characterized in that: described Paecilomyces Fumosoroseus strain belongs to hyphomycetales, paecilomyces.
3. a kind of Paecilomyces Fumosoroseus strain according to claim 1; it is characterized in that: the bacterium colony back side of described Paecilomyces Fumosoroseus strain is colourless or yellow; the conidiophore list is given birth to or is gathered into coremium, 100 * 1.5～2 μ m, and wall is smooth; transparent; the colyliform branch of forming whorl by living 4～6 bottles stalk is formed mostly, bottle metulae portion sphere, or intend ellipse and expand; top elongated, 5.7～8 * 1～2 μ m.The conidium cylindricality is to fusiformis, and is smooth, is clear to little incarnadine, 3～4 * 1～2 μ m, no chlamydospore.
4. the described a kind of Paecilomyces Fumosoroseus strain of claim 1 is prevented and treated application in Bemisia tabaci, Trialeurodes vaporariorum Westwood, aphid or the small cabbage moth medicine in preparation.
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| CN106879633A (en) * | 2015-12-15 | 2017-06-23 | 江苏省农业科学院 | It is a kind of to recycle the method that paecilomyces fumosoroseus prevents and treats Bemisia tabaci |
| CN113174335A (en) * | 2021-04-19 | 2021-07-27 | 广西大学 | Separation method of banana colletotrichum gloeosporioides |
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2007
- 2007-11-20 CN CNB200710031511XA patent/CN100557009C/en not_active Expired - Fee Related
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| CN103444786B (en) * | 2013-08-22 | 2016-04-20 | 广东省农业科学院植物保护研究所 | A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method |
| CN103444786A (en) * | 2013-08-22 | 2013-12-18 | 广东省农业科学院植物保护研究所 | Bacillus thuringiensis and isaria fumosorosea compounded suspending agent and preparation method thereof |
| CN103798295B (en) * | 2013-12-03 | 2015-07-29 | 华南农业大学 | The compounded pesticides of paecilomyces fumosoroseus and Buprofezin |
| CN103798295A (en) * | 2013-12-03 | 2014-05-21 | 华南农业大学 | Paecilomyces fumosoroseus-buprofezin compound pesticide |
| CN104073440A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus and application thereof |
| CN104073440B (en) * | 2013-12-06 | 2016-06-15 | 江西天人生态股份有限公司 | One strain paecilomyces fumosoroseus and application thereof |
| CN103756913A (en) * | 2013-12-24 | 2014-04-30 | 华南农业大学 | Isaria fumosorosea |
| CN103756913B (en) * | 2013-12-24 | 2016-05-25 | 华南农业大学 | A kind of rose dark brown Isaria bacterial strain |
| CN106879633A (en) * | 2015-12-15 | 2017-06-23 | 江苏省农业科学院 | It is a kind of to recycle the method that paecilomyces fumosoroseus prevents and treats Bemisia tabaci |
| CN105483030A (en) * | 2016-01-21 | 2016-04-13 | 中国农业科学院烟草研究所 | Paecliomyces.sp strain for preventing and treating myzus persicae |
| CN106376600A (en) * | 2016-08-30 | 2017-02-08 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus wettable powder and preparation method thereof |
| CN113174335A (en) * | 2021-04-19 | 2021-07-27 | 广西大学 | Separation method of banana colletotrichum gloeosporioides |
| CN113174335B (en) * | 2021-04-19 | 2022-06-17 | 广西大学 | Separation method of banana colletotrichum gloeosporioides |
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