CN103444786B - A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method - Google Patents
A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method Download PDFInfo
- Publication number
- CN103444786B CN103444786B CN201310368992.9A CN201310368992A CN103444786B CN 103444786 B CN103444786 B CN 103444786B CN 201310368992 A CN201310368992 A CN 201310368992A CN 103444786 B CN103444786 B CN 103444786B
- Authority
- CN
- China
- Prior art keywords
- dark brown
- bacillus thuringiensis
- oil
- rose dark
- brown isaria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria, be made up of the raw material of following mass percent: bacillus thuringiensis,Bt 1 ~ 20%, rose dark brown Isaria 1 ~ 20%, vegetable oil 1 ~ 20%, emulsifier 3 ~ 8%, dispersant 0.1 ~ 5%, stabilizing agent 1 ~ 5%, antifreezing agent 1 ~ 5%, surplus is mineral oil or methyl oleate; The preparation method of this suspending agent is: 1) bacillus thuringiensis,Bt, rose dark brown Isaria, vegetable oil, emulsifier, dispersant, stabilizing agent, antifreezing agent and inert mineral oil or methyl oleate are mixed in proportion; 2) joining in clipper by said mixture, is 3000-10000 rev/min of condition down cut 15 minutes at rotating speed; 3) mixture after shearing is proceeded in sand mill grind.Beneficial effect of the present invention is: have active high, effect stability, the lasting period is long, environmental influence is little, easy to use advantage.
Description
Technical field
The invention belongs to public nuisance-free agricultural chemicals preparation technique field, be specifically related to a kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method.
Background technology
Bacillus thuringiensis,Bt English name bacillusthuringiensis, is called for short Bt, and being that the class comprising many mutation produces crystal bacillus, is the biological insecticides that current production rate is maximum, use is the widest.Its main active of bacillus thuringiensis,Bt is one or several insecticidal crystal proteins (insecticidalcrystalproteins, ICPs), also known as delta-endotoxin, to insects such as Lepidoptera, coleoptera, diptera, Hymenoptera, Homopteras, and the arthropods such as animals and plants nematode, acarian has specific cytotoxicity, and to nontarget organism safety.Therefore, Bt insecticide has single-minded, efficient and to advantages such as person poultry safeties.But commodity Bt preparation also demonstrates some limitation in production control, and as poor in quick-acting, insensitive on high instar larvae, the field lasting period is short etc. has become the restraining factors affecting Bt and successfully promote the use of further all.
Rose dark brown Isaria (Isariafumosorosea) is a kind of ascomycetes entomogenous fungi, and it blazons the whole world, and host range is extensive, has recorded 25 not equal insects, has comprised some Important Agricultural insects such as diamond-back moth, Diuraphis noxia and Bemisia tabaci.Rose dark brown Isaria conidium, mainly through secreting the outer hydrolase insect body wall of multiple born of the same parents thus invading host, has the mechanism of action being different from existing chemical insecticide, environmentally safe, the feature of noresidue.But the fungi comprising rose dark brown Isaria all has the shortcoming that insecticidal effect is slow, affected by environment comparatively greatly, effect is unstable.
There is very high interfacial tension between insect lipophilicity epidermis and the medicament being watered use, allow medicament be difficult to insect and plant surface affine.Irrational preparation, not only causes the death of part thalline or spore inactivation, and greatly can reduce the performance of its effect, thus affects lasting effect.Oil-suspending agent " OD " has good dispersion, stable performance, tackness is good, obvious to target synergistic effect, drug effect performance is more abundant, be convenient to the advantages such as mechanically actuated, and fungus conidium can keep permanent stability in oil-suspending agent simultaneously.
Summary of the invention
The object of this invention is to provide a kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method, to overcome prior art above shortcomings.
The object of the invention is to be achieved through the following technical solutions:
A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria, be made up of the raw material of following mass percent: bacillus thuringiensis,Bt 1 ~ 20%, rose dark brown Isaria 1 ~ 20%, vegetable oil 1 ~ 20%, emulsifier 3 ~ 8%, dispersant 0.1 ~ 5%, stabilizing agent 1 ~ 5%, antifreezing agent 1 ~ 5%, surplus is mineral oil or methyl oleate;
Preferably, described suspending agent is made up of the raw material of following mass percent: bacillus thuringiensis,Bt 10 ~ 20%, rose dark brown Isaria 10 ~ 20%, vegetable oil 10 ~ 15%, emulsifier 3 ~ 5%, dispersant 1%, stabilizing agent 3%, antifreezing agent 2 ~ 4%, surplus is mineral oil or methyl oleate; Preferred, described suspending agent is made up of the raw material of following mass percent: bacillus thuringiensis,Bt 15%, rose dark brown Isaria 15%, vegetable oil 15%, emulsifier 4%, dispersant 1%, stabilizing agent 3%, antifreezing agent 4%, and surplus is mineral oil or methyl oleate;
Described vegetable oil can be one or more the combination in soybean oil, rapeseed oil, castor oil, corn oil, peanut oil and plant blend oil.
Described emulsifier can be alkylphenol polyoxyethylene, styryl phenol polyethenoxy ether, alkylaryl polyoxyethylene polyoxypropylene ether, alkaryl phenol polyethenoxy ether phosphate, castor oil polyoxyethylene ether, alkylbenzenesulfonate, alkylphenol-polyethenoxy phosphate, polyoxyethylene sorbitan fatty acid ketone, sorbitan ester, fatty alcohol-polyoxyethylene ether, disecoctylmaleate sodium sulfonate, two or more combination in polyol fatty acid ester and ethylene oxide adduct thereof and lauryl sodium sulfate.
Described dispersant can be the combination of one or more in alkylphenol polyoxyethylene formaldehyde condensation products sulphate, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate and sodium lignin sulfonate.
Described stabilizing agent can be one or both the combination in 2,6-di-tert-butyl-4-methy phenol, epoxidized soybean oil, epoxidized linseed oil, epoxy fat and pentaerythrite.
Described antifreezing agent can be one or both the combination in ethylene glycol, propane diols, glycerine and diethylene glycol.
Described mineral oil can be kerosene, diesel oil or White Mineral Oil.
The preparation method of above-mentioned bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria, comprises the following steps:
1) bacillus thuringiensis,Bt, rose dark brown Isaria, vegetable oil, emulsifier, dispersant, stabilizing agent, antifreezing agent and inert mineral oil or methyl oleate are mixed in proportion;
2) joining in clipper by said mixture, is 3000-10000 rev/min of condition down cut 15 minutes at rotating speed; And
3) mixture after shearing is proceeded in sand mill grind.
Preferably, shear rate is 5000 revs/min, and the environmental temperature of process of lapping is no more than 40 DEG C.
Beneficial effect of the present invention is: by bacillus thuringiensis,Bt and rose dark brown Isaria composite, improve preventive effect, and reduce formulation interface tension force by the combination of emulsifier and vegetable oil, medicament and insect body wall and plant leaf blade are had well affine, resistance of rainwater washing against; Utilize mineral oil or methyl oleate to be combined with microorganism and synergistic effect is produced to pest control, improve Conidia persistence, fungal infection speed is significantly promoted, greatly improve quick-acting and the insecticidal activity of medicament, meanwhile, add the dispersant of suitable proportion, stabilizing agent, antifreezing agent and mineral oil, preparation is allowed to have better chemistry and physical stability, improve Conidia persistence, extend its operating period, meet the needs of agricultural insect pests control well.A kind of bacillus thuringiensis,Bt of the present invention and the composite suspending agent of rose dark brown Isaria are efficient, low toxicity, to environment noresidue, person poultry safety, disease is not easily developed immunity to drugs, be highly suitable for the control of insect of fruit tree, vegetables, wheat, paddy rice and flowers and other crops, especially efficient to Lepidoptera, diptera.After testing, oil-suspending agent of the present invention preserves 2 years spore germination rates is 80%.
Embodiment
Embodiment 1
1, take bacillus thuringiensis,Bt 10g, the rose dark brown cluster cryptogam 10.0g of 1000000000/gram, peanut oil 5.0g, castor oil polyoxyethylene ether 3.0g, fatty alcohol-polyoxyethylene ether 2.0g, alkyl phenol formaldehyde resin polyoxyethylene ether sodium sulphate 1.0g, epoxidized linseed oil 2.0g, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate and ethylene glycol 4.0g, White Mineral Oil complements to 100g, 4000 revs/min, shear 15 minutes, then move on in grinder, with water cooling in process of lapping, temperature can not more than 40 DEG C, 3000 revs/min are ground 30 minutes, obtained mass percent concentration is bacillus thuringiensis,Bt and the composite oil-suspending agent of rose dark brown Isaria of 20%.
2,20% rose dark brown Isaria oil-suspending agent toxicity determination experiment
For examination object: cabbage caterpillar, the sensitive strain of indoor feeding;
Reagent agent: the bacillus thuringiensis,Bt of 20% and the composite oil-suspending agent of rose dark brown Isaria, 20% rose dark brown Isaria oil-suspending agent, 20% bacillus thuringiensis,Bt oil suspension;
Condition: temperature: 25 DEG C, humidity: RH70%, illumination: L:D=12:12
Test method: accurately take reagent agent respectively with electronic analytical balance, dilute with water 800 times.
Experiment adopts spray-on process to process, and the rape leave in culture dish will access healthy and strong cabbage caterpillar 20, the liquid diluted is aimed at polypide spraying, puts into incubator with gauze capping.Take clear water as blank, each process repeats for 3 times, checks dead, borer population of living after 96 hours.After the bacillus thuringiensis,Bt of the present invention 20% and the composite oil-suspending agent of rose dark brown Isaria 800 times of medicines, 96 hours indoor control effects are 88.6%, and single dose rose dark brown Isaria and bacillus thuringiensis,Bt oil suspension use according to same method, after medicine, 96 hours control efficiency are respectively 50%, 63.2%, illustrate that the present invention is obviously better than single dose rose dark brown Isaria and bacillus thuringiensis,Bt oil suspension to the preventive effect of cabbage caterpillar, the results are shown in Table 1:
Table 1
Embodiment 2
1, take bacillus thuringiensis,Bt 20.0g, the rose dark brown cluster cryptogam 20.0g of 1000000000/gram, peanut oil 15.0g, polyoxyethylene sorbitan fatty acid ketone 2.0g, alkylphenol polyoxyethylene 1.0g, fatty alcohol-polyoxyethylene ether 1.0g, alkylphenol polyoxyethylene formaldehyde condensation products sodium sulphate 1.0g, epoxidized soybean oil 3.0g, glycerine 4.0g, surplus methyl oleate complements to 100g, 5000 revs/min, shear 15 minutes, then move on in grinder, with water cooling in process of lapping, temperature can not more than 40 DEG C, 3000 revs/min are ground 30 minutes, obtained mass percent concentration is bacillus thuringiensis,Bt and the composite oil-suspending agent of rose dark brown Isaria of 40%.
2, toxicity determination experiment method is with embodiment 1.
Described bacillus thuringiensis,Bt and rose dark brown Isaria oil-suspending agent are diluted spraying cigarette aphids by 1500 times, after medicine, 96 hours indoor control effects are 90%, single dose 40% rose dark brown Isaria and 40% bacillus thuringiensis,Bt oil suspension use according to same method, and after medicine, 96 hours control efficiency are respectively 70%, 73%.The results are shown in Table 2:
Table 2
Embodiment 3
1, take bacillus thuringiensis,Bt 20.0g, 1000000000/gram of rose dark brown cluster cryptogam 1.0g, peanut oil 10.0g, alkaryl phenol polyethenoxy ether phosphate 1.0g, castor oil polyoxyethylene ether 1.5g, sodium alkyl benzene sulfonate 1.0g, epoxidized linseed oil 2.0g, sodium lignin sulfonate 1.0g, ethylene glycol 3.0g, surplus methyl oleate complements to 100g, 5000 revs/min, shear 15 minutes, then move on in grinder, with water cooling in process of lapping, temperature can not more than 40 DEG C, 3000 revs/min are ground 30 minutes, obtained mass percent concentration is bacillus thuringiensis,Bt and the composite oil-suspending agent of rose dark brown Isaria of 21%.
2, toxicity determination experiment method is with embodiment 1.
Described bacillus thuringiensis,Bt and rose dark brown Isaria oil-suspending agent are diluted spraying cigarette aphids by 1200 times, after medicine, 96 hours indoor control effects are 90%, single dose 21% rose dark brown Isaria and 21% bacillus thuringiensis,Bt oil suspension use according to same method, and after medicine, 96 hours control efficiency are respectively 60%, 65%.The results are shown in Table 3:
Table 3
Embodiment 4
1, take bacillus thuringiensis,Bt 1.0g, 1000000000/gram of rose dark brown cluster cryptogam 20.0g, soybean oil 10.0g, alkaryl phenol polyethenoxy ether phosphatase 11 .0g, castor oil polyoxyethylene ether 1.5g, sodium alkyl benzene sulfonate 1.0g, epoxidized linseed oil 2.0g, sodium lignin sulfonate 1.0g, ethylene glycol 3.0g, surplus methyl oleate complements to 100g, 5000 revs/min, shear 15 minutes, then move on in grinder, with water cooling in process of lapping, temperature can not more than 40 DEG C, 3000 revs/min are ground 30 minutes, obtained mass percent concentration is bacillus thuringiensis,Bt and the composite oil-suspending agent of rose dark brown Isaria of 21%.
2, toxicity determination experiment method is with embodiment 1.
Described bacillus thuringiensis,Bt and rose dark brown Isaria oil-suspending agent are diluted spraying cigarette aphids by 1200 times, after medicine, 96 hours indoor control effects are 85%, single dose 21% rose dark brown Isaria and 21% bacillus thuringiensis,Bt oil suspension use according to same method, and after medicine, 96 hours control efficiency are respectively 60%, 65%.The results are shown in Table 4:
Table 4
The present invention is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present invention; no matter but any change is done in its shape or structure; every have identical with the application or akin technical scheme, all drops within protection scope of the present invention.
Claims (10)
1. bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria, is characterized in that, be made up of the raw material of following mass percent: bacillus thuringiensis,Bt 1 ~ 20%, rose dark brown Isaria 1 ~ 20%, vegetable oil 1 ~ 20%, emulsifier 3 ~ 8%, point
Powder 0.1 ~ 5%, stabilizing agent 1 ~ 5%, antifreezing agent 1 ~ 5%, surplus is mineral oil or methyl oleate;
Wherein, bacillus thuringiensis,Bt unit is 50000IU/mg, and rose dark brown Isaria spore content is 10 ~ 10,000,000,000/gram.
2. bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria according to claim 1, it is characterized in that, be made up of the raw material of following mass percent: bacillus thuringiensis,Bt 10 ~ 20%, rose dark brown Isaria 10 ~ 20%, vegetable oil 10 ~ 15%, emulsifier 3 ~ 5%, dispersant 1%, stabilizing agent 3%, antifreezing agent 2 ~ 4%, surplus is mineral oil or methyl oleate.
3. bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria according to claim 2, it is characterized in that, be made up of the raw material of following mass percent: bacillus thuringiensis,Bt 15%, rose dark brown Isaria 15%, vegetable oil 15%, emulsifier 4%, dispersant 1%, stabilizing agent 3%, antifreezing agent 4%, surplus is mineral oil or methyl oleate.
4. the bacillus thuringiensis,Bt according to any one of claim 1-3 and the composite suspending agent of rose dark brown Isaria, is characterized in that: described vegetable oil is one or more the combination in soybean oil, rapeseed oil, castor oil, corn oil, peanut oil and plant blend oil.
5. bacillus thuringiensis,Bt according to claim 1 and the composite suspending agent of rose dark brown Isaria, it is characterized in that: described emulsifier is alkylphenol polyoxyethylene, styryl phenol polyethenoxy ether, alkylaryl polyoxyethylene polyoxypropylene ether, alkaryl phenol polyethenoxy ether phosphate, castor oil polyoxyethylene ether, alkylbenzenesulfonate, alkylphenol-polyethenoxy phosphate, polyoxyethylene sorbitan fatty acid ketone, fatty alcohol-polyoxyethylene ether, disecoctylmaleate sodium sulfonate, two or more combination in polyol fatty acid ester and ethylene oxide adduct thereof and lauryl sodium sulfate.
6. bacillus thuringiensis,Bt according to claim 1 and the composite suspending agent of rose dark brown Isaria, is characterized in that: described dispersant is the combination of one or more in alkylphenol polyoxyethylene formaldehyde condensation products sulphate, di-2-ethylhexylphosphine oxide bitter edible plant sodium sulfonate and sodium lignin sulfonate.
7. bacillus thuringiensis,Bt according to claim 1 and the composite suspending agent of rose dark brown Isaria, it is characterized in that: described stabilizing agent is one or both the combination in 2,6-di-tert-butyl-4-methy phenol, epoxidized soybean oil, epoxidized linseed oil, epoxy fat and pentaerythrite.
8. bacillus thuringiensis,Bt according to claim 1 and the composite suspending agent of rose dark brown Isaria, is characterized in that: described antifreezing agent is one or both the combination in ethylene glycol, propane diols, glycerine and diethylene glycol.
9. bacillus thuringiensis,Bt according to claim 1 and the composite suspending agent of rose dark brown Isaria, is characterized in that: described mineral oil is kerosene, diesel oil or White Mineral Oil.
10. the preparation method of the bacillus thuringiensis,Bt described in any one of claim 1-9 and the composite suspending agent of rose dark brown Isaria, is characterized in that, comprise the following steps:
1) by bacillus thuringiensis,Bt, rose dark brown Isaria, vegetable oil, emulsifier, dispersant, stabilizing agent, antifreezing agent and inert mineral
Oil or methyl oleate mix in proportion;
2) joining in clipper by said mixture, is 3000-10000 rev/min of condition down cut 15 minutes at rotating speed;
And
3) mixture after shearing is proceeded in sand mill grind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310368992.9A CN103444786B (en) | 2013-08-22 | 2013-08-22 | A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310368992.9A CN103444786B (en) | 2013-08-22 | 2013-08-22 | A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103444786A CN103444786A (en) | 2013-12-18 |
| CN103444786B true CN103444786B (en) | 2016-04-20 |
Family
ID=49727971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310368992.9A Expired - Fee Related CN103444786B (en) | 2013-08-22 | 2013-08-22 | A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103444786B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766407B (en) * | 2013-12-24 | 2015-11-18 | 华南农业大学 | Rose dark brown Isaria and Avermectin oil-suspending agent and its preparation method and application |
| CN103749545B (en) * | 2013-12-24 | 2015-09-30 | 华南农业大学 | Rose dark brown Isaria and bacillus thuringiensis,Bt wetting powder |
| CN103749441B (en) * | 2013-12-24 | 2015-01-21 | 华南农业大学 | Isaria fumosorosea wettable powder as well as preparation method and application thereof |
| CN103749546B (en) * | 2013-12-24 | 2015-12-30 | 华南农业大学 | A kind of rose dark brown Isaria and beta-cypermethrin oil-suspending agent and application thereof |
| US20190014786A1 (en) * | 2015-12-28 | 2019-01-17 | Novozymes Bioag A/S | Stable inoculant compositions and methods for producing same |
| CN106034864A (en) * | 2016-05-24 | 2016-10-26 | 安徽天酬林业有限公司 | Summer Black grape dirt-resistant oil film and preparation technology therefor |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN102246750A (en) * | 2011-05-20 | 2011-11-23 | 华南农业大学 | Isaria fumosorosea oil suspending agent and preparation method as well as application thereof |
| CN102948423A (en) * | 2012-11-12 | 2013-03-06 | 辽宁海佳农化有限公司 | Herbicide compound containing mesotrione and terbuthylazine and preparation method thereof |
-
2013
- 2013-08-22 CN CN201310368992.9A patent/CN103444786B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN102246750A (en) * | 2011-05-20 | 2011-11-23 | 华南农业大学 | Isaria fumosorosea oil suspending agent and preparation method as well as application thereof |
| CN102948423A (en) * | 2012-11-12 | 2013-03-06 | 辽宁海佳农化有限公司 | Herbicide compound containing mesotrione and terbuthylazine and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103444786A (en) | 2013-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103444786B (en) | A kind of bacillus thuringiensis,Bt and the composite suspending agent of rose dark brown Isaria and preparation method | |
| CN102246750A (en) | Isaria fumosorosea oil suspending agent and preparation method as well as application thereof | |
| CN102669192A (en) | Matrine microcapsule suspension agent and preparation method thereof | |
| CN106489964A (en) | Spiral shell worm ethyl ester containing mineral oil dispersibles oil-suspending agent and preparation method thereof, application | |
| CN103766407B (en) | Rose dark brown Isaria and Avermectin oil-suspending agent and its preparation method and application | |
| CN102405914B (en) | Synergistic mite killing composition containing cyflumetofen and clofentezine | |
| CN102177887A (en) | Diafenthiuron emulsion in water and preparation method thereof | |
| CN101984828A (en) | Compound pesticide | |
| CN109452313A (en) | A kind of entomopathogenic nematode preparation and preparation method and application | |
| CN103828842B (en) | Camellia Leaves extract and Metarhizium anisopliae compounded pesticides and preparation method thereof | |
| CN100490647C (en) | Insecticide composition | |
| CN101642108A (en) | Pesticidal composition with synergistic effect | |
| CN101999395A (en) | Insecticidal composition containing pymetrozine | |
| Maranguape et al. | Castor cake as organic fertilizer to control gastrointestinal nematodes in pasture-raised sheep | |
| CN102696655A (en) | Compound pesticide composition containing spirodiclofen and preparation thereof | |
| CN102224831B (en) | Control method of tetranychus cinnabarinus based on tomato extracts | |
| CN102696631B (en) | Synergistic acaricidal composition containing etoxazole and chlorfenapyr | |
| CN111727970A (en) | Pesticide containing furan compound and application thereof | |
| CN104255751A (en) | Mite-killing composition containing etoxazole and vermectin | |
| CN103271078A (en) | Etoxazole-profenofos pesticide composition | |
| CN103931617B (en) | A kind of containing flonicamid with the composition pesticide of cyflumetofen | |
| CN102599176B (en) | Barbadosnut phorbol ester water emulsion insecticide and preparation method thereof | |
| CN103271042B (en) | Etoxazole-hexythiazomiticide miticide composition | |
| Li | Beauveria bassiana for pine caterpillar management in the People’s Republic of China | |
| CN101978832A (en) | Pesticide composition containing dinotefuran and chlorpyrifos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160420 Termination date: 20170822 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |