CN106376600A - Paecilomyces fumosoroseus wettable powder and preparation method thereof - Google Patents
Paecilomyces fumosoroseus wettable powder and preparation method thereof Download PDFInfo
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- CN106376600A CN106376600A CN201610790631.7A CN201610790631A CN106376600A CN 106376600 A CN106376600 A CN 106376600A CN 201610790631 A CN201610790631 A CN 201610790631A CN 106376600 A CN106376600 A CN 106376600A
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- Prior art keywords
- paecilomyces fumosoroseus
- wettable powder
- parts
- paecilomyces
- fumosoroseus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
Abstract
The invention provides a Paecilomyces fumosoroseus wettable powder. The Paecilomyces fumosoroseus wettable powder comprises, by weight, 10-20 parts of a medicinal active component which is Paecilomyces fumosoroseus, 14-44 parts of an assistant and 40-70 parts of a carrier. The Paecilomyces fumosoroseus wettable powder has the advantages of high efficiency, low toxicity, stable insecticidal effect, safety to people, animals and crops, and high safety. An experiment result shows that the pest disease control effect of the Paecilomyces fumosoroseus wettable powder reaches up to 79% or above.
Description
Technical field
The present invention relates to technical field of pesticide is and in particular to arrive a kind of paecilomyces fumosoroseus wettable powder and preparation side
Method.
Background technology
China is large agricultural country, and for thousands of years always based on planting industry, agricultural is the source of basic necessities of life of the mankind, survives it
This, be that other departments are able to independent and development basis, be the basis of national economy, the development of therefore agricultural will directly affect
The development of national economy.But in agricultural production, the generation of insect pest often has a strong impact on crop growth, make crops from
Physiological function there occurs a series of change to institutional framework and destroys, and then leads to a large amount of underproduction of crops, carries to grower
Carry out huge economic loss.Therefore the effective preventing and treating to crop pests is instant, to ensure grain security and kind
The economic interests of plant person.
At present the species of crop pests has many kinds, wherein topmost insect pest be hexapeopoda pest, lepidoptera pest,
Hymenoptera insect, Hemipteran pest and coleopteran pest, such as Bemisia tabaci, trialeurodes vaporariorum, diamondback moth, cabbage caterpillar, bollworm, corn
Snout moth's larva, black peach aphid etc., the generation of these insect pests, cause a large amount of underproduction of crops and lead to the quality of crops to reduce.In prior art,
Usually carry out pest control disease from disease-resistant variety or chemical insecticide.When being prevented and treated using chemical insecticide, prevention effect
Although preferable, chemical insecticide has difficult degradation, is easily destroyed the balance of the ecosystem, people and animals is endangered larger.Therefore,
It is badly in need of a kind of efficient biological insecticides of preventing and treating and carry out pest control.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide a kind of paecilomyces fumosoroseus can
WP and preparation method thereof.
The invention provides a kind of paecilomyces fumosoroseus wettable powder, including the component of following parts by weight：
10～20 parts of medicine activity component, described medicine activity component is paecilomyces fumosoroseus；
14～44 parts of auxiliary agent；
40～70 parts of carrier.
Preferably, described paecilomyces fumosoroseus number selected from biological deposits be CGMCC No.7993 bacterial classification.
Preferably, effective spore amount of described paecilomyces fumosoroseus wettable powder is 1.45～1.55 × 1010cfu/g.
Preferably, miscellaneous bacteria rate≤0.1% of described paecilomyces fumosoroseus wettable powder.
Preferably, suspensibility >=80% of described paecilomyces fumosoroseus wettable powder.
Preferably, spore rate >=90% alive of the paecilomyces fumosoroseus of described paecilomyces fumosoroseus wettable powder.
Preferably, described auxiliary agent include fatty alcohol and ethylene oxide condensate, naphthalene sulphonate, wetting agent EFW and
Wooden sodium.
Preferably, on the basis of the parts by weight of auxiliary agent, described auxiliary agent includes fatty alcohol and ethylene oxide condensate 5～15
Part, 3～8 parts of naphthalene sulphonate, 1～6 part of wetting agent EFW and wooden 5～15 parts of sodium.
Preferably, described carrier includes calcium carbonate, diatomite, talcum powder, white carbon, bentonite, sodium sulphate, potter's clay and height
One or more of ridge soil.
The invention provides the preparation method of the paecilomyces fumosoroseus oil-suspending agent of technique scheme, walk including following
Suddenly：
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains paecilomyces fumosoroseus wettable powder.
The invention provides a kind of paecilomyces fumosoroseus wettable powder, including the component of following parts by weight：Medicine is lived
Property 10～20 parts of component, described medicine activity component is paecilomyces fumosoroseus；14～44 parts of auxiliary agent；40～70 parts of carrier.?
In the present invention, paecilomyces fumosoroseus wettable powder is efficient, low toxicity, and insecticidal effect is stable, and to people and animals and crop safety, safety
Property high.Test result indicate that, the effect of paecilomyces fumosoroseus wettable powder pest control reaches as high as more than 79%.
Biological deposits explanation
Paecilomyces fumosoroseus (Paecilomyces fumosa-roseus), is deposited in Chinese microorganism strain preservation management
Committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, it is CGMCC No.7993 that biological deposits are numbered.
Specific embodiment
The invention provides a kind of paecilomyces fumosoroseus wettable powder, including the component of following parts by weight：Medicine is lived
Property 10～20 parts of component, described medicine activity component be paecilomyces fumosoroseus；14～44 parts of auxiliary agent；40～70 parts of carrier.
The paecilomyces fumosoroseus wettable powder that the present invention provides preferably includes 10～20 parts of paecilomyces fumosoroseus, more excellent
Elect 13～18 parts as, most preferably 15 parts.
In the present invention, described paecilomyces fumosoroseus is preferably selected from biological deposits and numbers being the bacterium of CGMCC No.7993
Plant activation culture to obtain.
In the present invention, the preparation method of described paecilomyces fumosoroseus comprises the following steps：
1) Paecilomyces Fumosoroseus kind is activated；
2) paecilomyces fumosoroseus after described activation is inoculated in paecilomyces fumosoroseus fluid nutrient medium and carries out liquid and send out
Ferment, obtains paecilomyces fumosoroseus zymotic fluid；
3) by described step 2) the paecilomyces fumosoroseus zymotic fluid that obtains is inoculated in paecilomyces fumosoroseus solid medium,
Carry out solid fermentation, obtain paecilomyces fumosoroseus.
In the present invention, the activation of Paecilomyces Fumosoroseus kind is that the Paecilomyces Fumosoroseus kind of preservation is inoculated in equipped with battalion
In the eggplant type bottle of foster agar medium, 25～28 DEG C of culture 8～12h carry out activation culture.
In the present invention, described activation after paecilomyces fumosoroseus be inoculated in fluid nutrient medium inoculum concentration be preferably 5～
15%, more preferably 7～12%, most preferably 10%；Described paecilomyces fumosoroseus zymotic fluid is inoculated in connecing of solid medium
The amount of kind is preferably 5～15%, more preferably 7～12%, most preferably 10%.
In the present invention, described paecilomyces fumosoroseus fluid nutrient medium preferably includes following components：Dehydrated potato powder 27～
39g/L, white granulated sugar 15～25g/L, peptone 0.5～1.5g/L, sodium nitrate 0.05～0.15g/L, potassium chloride 0.20～
0.30g/L, magnesium sulfate 0.01～0.10g/L, manganese sulfate 0.01～0.10g/L, dipotassium hydrogen phosphate 0.1～1.0g/L, ferric sulfate
0.01～0.10g/L；More preferably dehydrated potato powder 30～35g/L, white granulated sugar 17～23g/L, peptone 0.8～1.2g/L, nitre
Sour sodium 0.08～0.12g/L, potassium chloride 0.23～0.27g/L, magnesium sulfate 0.03～0.07g/L, manganese sulfate 0.03～0.07g/
L, dipotassium hydrogen phosphate 0.3～0.7g/L, ferric sulfate 0.03～0.07g/L；Most preferably dehydrated potato powder 32g/L, white granulated sugar 20g/
L, peptone 1.0g/L, sodium nitrate 0.10g/L, potassium chloride 0.25g/L, magnesium sulfate 0.05g/L, manganese sulfate 0.05g/L, phosphoric acid hydrogen
Dipotassium 0.5g/L, ferric sulfate 0.05g/L.
In the present invention, the fluid nutrient medium that described paecilomyces fumosoroseus liquid fermentation is inoculated is preferably through 120～130 DEG C
Use after sterilizing 30～45min.The present invention is not particularly limited to the sterilizing installation of culture medium, ripe using those skilled in the art
The sterilizing installation known, such as high-pressure steam sterilizing pan.The present invention does not have special restriction to the equipment of described fermentation, adopts
Zymolysis Equipment well known in the art, such as fermentation tank.
In the present invention, the temperature of described paecilomyces fumosoroseus liquid fermentation is preferably 25～30 DEG C, and more preferably 26
～29 DEG C, most preferably 28 DEG C；The pH value of described paecilomyces fumosoroseus liquid fermentation is preferably 6.6～7.2, more preferably
6.8～7.0, most preferably 6.9；Pressure inside the tank is preferably 0.05～0.1MPa, more preferably 0.07～0.08MpPa；Described
The throughput of liquid fermentation is preferably 0.8～1.2V/V min, more preferably 1V/V min；Described paecilomyces fumosoroseus liquid
The time of body fermentation is preferably 30～50h, more preferably 35～45h, more preferably 40h.
After completing described paecilomyces fumosoroseus liquid fermentation, the paecilomyces fumosoroseus obtaining zymotic fluid is inoculated in by the present invention
In solid medium, carry out solid fermentation, obtain paecilomyces fumosoroseus.In the present invention, described paecilomyces fumosoroseus solid training
Foster base preferably includes the component of following weight portion：60～100 parts of wheat bran, 5～15 parts of corn flour, 5～15 parts of husk；More preferably
70～90 parts of wheat bran, 7～12 parts of corn flour, 7～12 parts of husk；Most preferably 80 parts of wheat bran, 10 parts of corn flour, 10 parts of husk.
The present invention adds distilled water preferably in described paecilomyces fumosoroseus solid medium, makes solid medium water content be preferably 45
～55%, more preferably 47～52%, most preferably 50%, then carry out solid fermentation.The distillation to described interpolation for the present invention
Water does not have special restriction, using the conventional use of distilled water of those skilled in the art.
In the present invention, the temperature of described paecilomyces fumosoroseus solid fermentation is preferably 24～30 DEG C, and more preferably 26
～29 DEG C, most preferably 28 DEG C；The humidity of described paecilomyces fumosoroseus solid fermentation be preferably 40～95%, more preferably 55～
75%, most preferably 65%；The time of described paecilomyces fumosoroseus solid fermentation preferably 48～96h, more preferably 60～
84h, most preferably 72h.
In the present invention, the thickness of described paecilomyces fumosoroseus solid medium preferably 1～7cm, more preferably 2～
5cm, most preferably 3cm.
After completing described paecilomyces fumosoroseus solid fermentation, the present invention preferably will obtain the drying of solid fermentation culture medium, powder
Broken and sieve, obtain solid, powdery paecilomyces fumosoroseus.In the present invention, the temperature of described drying is preferably 30～50 DEG C, does
Preferably 20～45h of dry time, more preferably 35～45 DEG C, 30～40h, most preferably 40 DEG C, 35h；The present invention is by solid
Fermentation medium is dried to moisture preferably between 25～35%, and more preferably 27～32%, most preferably 30%.This
The bright method that described solid fermentation culture medium is pulverized does not have special restriction, using pulverizing side well known to those skilled in the art
Method.In the present invention, described solid, powdery paecilomyces fumosoroseus sieves and preferably employs 80～100 mesh, more preferably 85～
95 mesh, most preferably 90 mesh.
The paecilomyces fumosoroseus wettable powder that the present invention provides preferably includes 14～44 parts of auxiliary agents, and more preferably 22～35
Part, most preferably 28 parts.The present invention is not particularly limited to the species of described auxiliary agent and source, ripe using those skilled in the art
The commercial goods of the auxiliary agent of agricultural wettable pulvis known.On the basis of the parts by weight of auxiliary agent, it is preferably specifically 5
～15 parts of fatty alcohols and ethylene oxide condensate, 3～8 parts of naphthalene sulphonate, 1～6 part of wetting agent EFW and 5～15 parts of wood
Sodium, more preferably 8～12 parts fatty alcohols and ethylene oxide condensate, 4～6 parts of naphthalene sulphonate, 2～5 parts of wetting agent EFW
With 8～12 parts of wooden sodium, most preferably 10 parts fatty alcohols and ethylene oxide condensate, 5 parts of naphthalene sulphonate, 3 parts of wetting agents
EFW and 10 part of wooden sodium.In the present invention, described wetting agent EFW is alkylnaphthalene sulfonate and anion humectant mixture, this
The bright source to described wetting agent EFW is not particularly limited, using commercial goods well known to those skilled in the art.At this
In invention, described auxiliary agent makes medicine proterties stable, improves drug effect.
The paecilomyces fumosoroseus wettable powder that the present invention provides preferably includes 40～70 parts of carriers, and more preferably 48～62
Part, most preferably 57 parts.In the present invention, described carrier be preferably calcium carbonate, diatomite, talcum powder, white carbon, bentonite,
One or more of sodium sulphate, potter's clay and kaolin.The present invention does not have special restriction to the source of described carrier, using city
Sell commodity.In the present invention, described carrier is preferably specifically arbitrarily several in above-mentioned substance, can mix arbitrary proportion
Close.In the present invention, described carrier is specifically more preferably white carbon and the kaolin in above-mentioned substance, is preferably specifically
5～15 parts of white carbons and 35～55 parts of kaolin, more preferably 8～12 parts white carbons and 40～50 parts of kaolin, most preferably
10 parts of white carbons and 47 parts of kaolin.Carrier of the present invention has good medicine stability, also can improve the drug effect of medicine.
The invention provides the preparation method of paecilomyces fumosoroseus wettable powder described in technique scheme, including following
Step：
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains paecilomyces fumosoroseus wettable powder.
In the present invention, the mixing of described carrier, medicine activity component and auxiliary agent is preferably carrier, medicine activity component
It is divided into 3～5 parts with auxiliary agent respectively, more preferably 4 parts, after the 1st part of carrier is mixed with the 1st part of medicine activity component, then
Mix with the 1st part of auxiliary agent, then mix with the 2nd part of carrier again, then mix with the 2nd part of medicine activity component, then with the 2nd part of auxiliary agent
Mixing, by that analogy, until having added all of raw material, is further continued for preferably mixing 20～40min, more preferably 25～35min,
Most preferably 30min.The present invention does not have special restriction to the equipment of described mixing, using well known to those skilled in the art mixed
Conjunction equipment, such as mixing fill.
In the present invention, 10～45 μm are preferably to the granularity of described powder, more preferably 20～35 μm, most preferably 25
～30 μm.The present invention does not have special restriction to the condition of described pulverizing, using pulverization conditions well known to those skilled in the art
?.In the present invention, described pulverizing is specifically preferably pulverized for air-flowing type, and the sealing pressure that air-flowing type is pulverized is preferably
0.05～0.20MPa, more preferably 0.1～0.15MPa, most preferably 0.13MPa；The discharging sealing gland pressure that air-flowing type is pulverized is excellent
Elect 0.1～0.2MPa, more preferably 0.15MPa as；The pulse that air-flowing type is pulverized is preferably 0.3～0.7MPa, more preferably
For 0.4～0.6MPa, most preferably 0.5MPa；The feed pressure of air-flowing type preferably 0.7～0.9MPa, more preferably 0.75～
0.85MPa, most preferably 0.8MPa.The present invention does not have special restriction to the equipment of described pulverizing, using people in the art
Disintegrating apparatus known to member.
In the present invention, effective spore amount of described paecilomyces fumosoroseus wettable powder be preferably 1.45～1.55 ×
1010Cfu/g, more preferably 1.5 × 1010cfu/g.In the present invention, the spore rate alive of described paecilomyces fumosoroseus wettable powder
>=90%；Miscellaneous bacteria rate≤0.1% of described paecilomyces fumosoroseus wettable powder；Described paecilomyces fumosoroseus wettable powder
Suspensibility >=80%.
Paecilomyces fumosoroseus wettable powder of the present invention can be applicable to the aspect of crops pest control, there is provided one
Plant application in terms of preventing and treating crop pests for the described paecilomyces fumosoroseus wettable powder.Specifically, applicable crops
Species includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Paecilomyces fumosoroseus of the present invention
Oil-suspending agent can prevent and treat each class pest and evil mite, including cabbage caterpillar, diamondback moth, bollworm, corn borer, chafer, red spider, pine
Caterpillar, wide abdomen mantis, aphid and subterranean pest-insect grub, mole cricket etc..
The present invention is not particularly limited to the application method of described paecilomyces fumosoroseus wettable powder, using this area
Application method known to technical staff.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the reality in the present invention
Apply example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all belong to
In the scope of protection of the invention.
Embodiment 1
The preparation of paecilomyces fumosoroseus：The Paecilomyces Fumosoroseus kind of preservation is inoculated in equipped with nutrient agar
In eggplant type bottle, 25 DEG C of culture 14h carry out activation culture；Paecilomyces fumosoroseus after activation culture is inoculated in 10% inoculum concentration
In fluid nutrient medium after 121 DEG C of sterilizing 35min, fermentation tank carries out liquid fermentation, specific fermentation condition is：Temperature is
25℃；PH value is 6.5；Pressure inside the tank is 0.08MPa, and fermentation throughput is 1V/V min；Stop fermentation after fermentation 45h, obtain
Paecilomyces fumosoroseus zymotic fluid；The paecilomyces fumosoroseus obtaining zymotic fluid is inoculated in solid medium with 10% inoculum concentration
In, add distilled water in solid medium, make solid medium water content be 55%, then carry out solid fermentation, ferment bar
Part is：Fermentation temperature is 25 DEG C, and fermentation humidity is 60%, and fermentation time is 72h；The solid obtaining is sent out after terminating by solid fermentation
The paecilomyces fumosoroseus of ferment dries 35h under the conditions of 40 DEG C, and the moisture drying the paecilomyces fumosoroseus to solid fermentation is
30%, pulverize 100 mesh sieves, obtain paecilomyces fumosoroseus.
Embodiment 2
The preparation of paecilomyces fumosoroseus wettable powder：In terms of parts by weight, by 10 parts of white carbons, 47 parts of kaolin, 15
The paecilomyces fumosoroseus of part embodiment 1 preparation, 10 parts of fatty alcohols and ethylene oxide condensate, 5 parts of naphthalene sulphonate, 3 parts
The wooden sodium of wetting agent EFW and 10 parts are divided into 4 heaps respectively, after the 1st heap white carbon is mixed with the 1st heap kaolin, then with the 1st heap
Paecilomyces fumosoroseus mixes, then mixes with ethylene oxide condensate with the 1st heap fatty alcohol, then with the 1st heap naphthalene sulphonate
Mixing, then mix with the 1st heap wetting agent EFW, then mix with the 1st heap wood sodium, then mix with the 2nd heap white carbon, by that analogy, directly
All mix to all raw materials, be further continued for mixing 30min, obtain compound；Compound is pulverized through air-flowing type, obtains 25 μm
Powder, that is, obtain paecilomyces fumosoroseus wettable powder.
The quality control index of the paecilomyces fumosoroseus wettable powder of table 1 embodiment 2 preparation
Embodiment 3
The preparation of paecilomyces fumosoroseus wettable powder：In terms of parts by weight, by 12 parts of white carbons, 50 parts of kaolin, 10
The paecilomyces fumosoroseus of part embodiment 1 preparation, 8 parts of fatty alcohols and ethylene oxide condensate, 8 parts of naphthalene sulphonate, 4 parts of profits
The wooden sodium of humectant EFW and 8 parts are divided into 4 heaps respectively, after the 1st heap white carbon is mixed with the 1st heap kaolin, then with the 1st heap rose
Dark brown Paecilomyces varioti mixes, then mixes with ethylene oxide condensate with the 1st heap fatty alcohol, then mixes with the 1st heap naphthalene sulphonate
Close, then mix with the 1st heap wetting agent EFW, then mix with the 1st heap wood sodium, then mix with the 2nd heap white carbon, by that analogy, until
All raw materials have all mixed, and are further continued for mixing 20min, obtain compound；Compound is pulverized through air-flowing type, obtains 20 μm of powder
Material, that is, obtain paecilomyces fumosoroseus wettable powder.
Embodiment 4
The preparation of paecilomyces fumosoroseus wettable powder：In terms of parts by weight, by 8 parts of white carbons, 50 parts of kaolin, 12 parts
The paecilomyces fumosoroseus of embodiment 1 preparation, 10 parts of fatty alcohols and ethylene oxide condensate, 6 parts of naphthalene sulphonate, 3 parts of profits
The wooden sodium of humectant EFW and 9 parts are divided into 4 heaps respectively, after the 1st heap white carbon is mixed with the 1st heap kaolin, then with the 1st heap rose
Dark brown Paecilomyces varioti mixes, then mixes with ethylene oxide condensate with the 1st heap fatty alcohol, then mixes with the 1st heap naphthalene sulphonate
Close, then mix with the 1st heap wetting agent EFW, then mix with the 1st heap wood sodium, then mix with the 2nd heap white carbon, by that analogy, until
All raw materials have all mixed, and are further continued for mixing 30min, obtain compound；Compound is pulverized through air-flowing type, obtains 30 μm of powder
Material, that is, obtain Paecilomyces lilacinus granule.
Embodiment 5
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is Chinese cabbage, and management is good, and cabbage caterpillar occurs medium
Lay particular stress on.Experimental field is divided into three regions by this test, and region 1 is control group, a spray clear water；Region 2 sprays embodiment 2
2000 times of dilutions of the paecilomyces fumosoroseus wettable powder of middle preparation；Region 3 sprays the 2000 times of dilutions of 3% effective cypermethrin
Liquid, the concentration of medicine activity component is identical with region 2；Trizonal dosage is identical, 1 day after dispenser, 3 days, and each investigation in 7 days
Once borer population alive, calculates Revision insect recluced rate.Concrete outcome as shown in table 2, can show, the insect population in region 2 subtracts by the data of table 2
Move back rate highest, show that the insecticidal effect of the paecilomyces fumosoroseus oil-suspending agent of present invention preparation is best.
Desinsection (cabbage caterpillar) effect of table 2 paecilomyces fumosoroseus wettable powder
Can draw from the test data of table 2, the paecilomyces fumosoroseus wettable powder insect population of 7 days of present invention preparation subtracts
The rate of moving back may be up to 79.1%, and its insecticidal effect is higher than effective cypermethrin.
As seen from the above embodiment, the paecilomyces fumosoroseus wettable powder of present invention preparation, the control rate to cabbage caterpillar
May be up to more than 79%, insecticidal effect is higher than effective cypermethrin.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of paecilomyces fumosoroseus wettable powder is it is characterised in that include the component of following parts by weight：
10～20 parts of medicine activity component, described medicine activity component is paecilomyces fumosoroseus；
14～44 parts of auxiliary agent；
40～70 parts of carrier.
2. a kind of paecilomyces fumosoroseus wettable powder according to claim 1 is it is characterised in that described rose dark brown is intended
Mould number selected from biological deposits be CGMCC No.7993 bacterial classification.
3. paecilomyces fumosoroseus wettable powder according to claim 1 is it is characterised in that described paecilomyces fumosoroseus can
Effective spore amount of WP is 1.45～1.55 × 1010cfu/g.
4. the paecilomyces fumosoroseus wettable powder according to claims 1 to 3 any one is it is characterised in that described
Miscellaneous bacteria rate≤0.1% of paecilomyces fumosoroseus wettable powder.
5. paecilomyces fumosoroseus wettable powder according to claim 3 is it is characterised in that described paecilomyces fumosoroseus can
Suspensibility >=80% of WP.
6. the paecilomyces fumosoroseus wettable powder according to claim 3 or 5 is it is characterised in that described rose dark brown intends green grass or young crops
Spore rate >=90% alive of the paecilomyces fumosoroseus of mould wettable powder.
7. paecilomyces fumosoroseus wettable powder according to claim 1 is it is characterised in that described auxiliary agent includes fatty alcohol
With ethylene oxide condensate, naphthalene sulphonate, wetting agent EFW and wooden sodium.
8. the paecilomyces fumosoroseus wettable powder according to claim 1 or 7 is it is characterised in that weight portion with auxiliary agent
On the basis of number, described auxiliary agent includes fatty alcohol and 5～15 parts of ethylene oxide condensate, 3～8 parts of naphthalene sulphonate, wetting
1～6 part of agent EFW and wooden 5～15 parts of sodium.
9. the paecilomyces fumosoroseus wettable powder according to claim 1 or 7 is it is characterised in that described carrier includes carbon
One or more of sour calcium, diatomite, talcum powder, white carbon, bentonite, sodium sulphate, potter's clay and kaolin.
10. the preparation method of the paecilomyces fumosoroseus wettable powder described in claim 1～9 any one, walks including following
Suddenly：
1) carrier, medicine activity component and auxiliary agent are mixed, obtain compound；
2) by described step 1) compound that obtains pulverized, and obtains paecilomyces fumosoroseus wettable powder.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610790631.7A CN106376600A (en) | 2016-08-30 | 2016-08-30 | Paecilomyces fumosoroseus wettable powder and preparation method thereof |
Applications Claiming Priority (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN108770870A (en) * | 2018-06-13 | 2018-11-09 | 马瑞燕 | A kind of fumosorosea water dispersible granules and preparation method thereof |
| WO2021054818A1 (en) * | 2019-09-19 | 2021-03-25 | Universiti Putra Malaysia | Composition for suppressing pest infestation |
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| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN102919272A (en) * | 2012-11-07 | 2013-02-13 | 湖北省生物农药工程研究中心 | Water dispersible granule for bacillus subtilis and method for preparing same |
| CN104073440A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101182466A (en) * | 2007-11-20 | 2008-05-21 | 华南农业大学 | Paecilomyces Fumosoroseus strain and uses thereof |
| CN102919272A (en) * | 2012-11-07 | 2013-02-13 | 湖北省生物农药工程研究中心 | Water dispersible granule for bacillus subtilis and method for preparing same |
| CN104073440A (en) * | 2013-12-06 | 2014-10-01 | 江西天人生态股份有限公司 | Paecilomyces fumosoroseus and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489927A (en) * | 2016-08-31 | 2017-03-15 | 江西天人生态股份有限公司 | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof |
| CN108770870A (en) * | 2018-06-13 | 2018-11-09 | 马瑞燕 | A kind of fumosorosea water dispersible granules and preparation method thereof |
| CN108770870B (en) * | 2018-06-13 | 2021-06-18 | 山西农业大学 | Isaria fumosorosea water dispersible granule and preparation method thereof |
| WO2021054818A1 (en) * | 2019-09-19 | 2021-03-25 | Universiti Putra Malaysia | Composition for suppressing pest infestation |
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