CN106376602A - Paecilomyces lilacinus granule and preparation method thereof - Google Patents
Paecilomyces lilacinus granule and preparation method thereof Download PDFInfo
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- CN106376602A CN106376602A CN201610799378.1A CN201610799378A CN106376602A CN 106376602 A CN106376602 A CN 106376602A CN 201610799378 A CN201610799378 A CN 201610799378A CN 106376602 A CN106376602 A CN 106376602A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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Abstract
The invention provides a Paecilomyces lilacinus granule. The Paecilomyces lilacinus granule comprises 5-15 parts of a medicinal active component which is Paecilomyces lilacinus, 10-25 parts of an assistant, 50-90 parts of a medicine carrier and 15-25 parts of water. The effective spore amount of the Paecilomyces lilacinus granule is 1.8-2.2 * 10<8> cfu/g, the competitor rate is not greater than 0.1%, and the shedding rate is not greater than 3%. The Paecilomyces lilacinus granule has the advantages of high efficiency, low toxicity, stable insecticidal effect, safety to people, animals and crops, and high safety. An experiment result shows that the control effect of Paecilomyces lilacinus reaches up to 78% or above.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly, to a kind of Paecilomyces lilacinus granule and preparation method thereof.
Background technology
Nematicide can attack and parasitic plant, affected normal because nematicide absorbs endotrophic by invasion and attack and parasitic plant
Growth promoter, and the secretions of nematode metabolism can stimulate cell and the tissue of host plant, leads to plant deformity.Great majority are planted
Thing nematicide encroaches on the under ground portion of plant, such as root, tuber etc., and root can behave as dross, necrosis, root is short and thick and grows thickly, and leads to
The a large amount of underproduction of crops or quality degradation.Common nemas has root knot nematode disease, soybean cyst nematode, Semen Tritici aestivi
Grain nematicide, sweep stem nematode, aphelenchoides besseyi disease, Citrus Tylenchulus Semipenetrans disease etc..
In prior art, usually to prevent and treat nematodiasis from disease-resistant variety or medicine, when being prevented and treated using medicine,
Often ratio is relatively low for its prevention effect, and prevention effect is undesirable.800 times of solution of 90% trichlorphon are such as adopted to process, preventive effect
It is only 45.8%, 1.5% Abamectin 2.5kg/667m2Preventive effect be 39.6%, at 3% 800 times of Furadan solution
Reason, prevention effect is 72.7%, and the prevention effect reaching is all than relatively low.
Content of the invention
In view of this, present invention aims to the deficiencies in the prior art, and provide a kind of Paecilomyces lilacinus granule
Agent and preparation method thereof.
The invention provides a kind of Paecilomyces lilacinus granule, including the component of following parts by weight：
5～15 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
10～25 parts of adjuvant；
50～90 parts of pharmaceutical carrier；
15～25 parts of water.
Further, described Paecilomyces lilacinus number selected from biological deposits be CGMCC No.7994 strain.
Further, effective spore amount of described Paecilomyces lilacinus granule is 1.8～2.2 × 108cfu/g.
Further, miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus granule.
Further, the granularity of described Paecilomyces lilacinus granule is 400 μm～2200 μm.
Further, expulsion rate≤3% of described Paecilomyces lilacinus.
Further, described adjuvant includes glucose and/or corn starch.
Further, described pharmaceutical carrier includes in kieselguhr, Pulvis Talci, white carbon, sodium sulfate, potter's clay and Kaolin
Plant or several.
Further, the preparation method of described Paecilomyces lilacinus granule is it is characterised in that comprise the following steps：
1) pharmaceutical carrier, medicine activity component and adjuvant mixed, pulverize, obtain powder；
2) by described step 1) powder that obtains mixed with water, pelletize, obtains Paecilomyces lilacinus granule.
Further, application in terms of preventing and treating crops nematicide for the described Paecilomyces lilacinus granule.
The invention provides a kind of Paecilomyces lilacinus granule, including the component of following parts by weight：Medicine activity component 5
～15 parts, described medicine activity component is Paecilomyces lilacinus；10～25 parts of adjuvant；50～90 parts of pharmaceutical carrier；Water 15～
25 parts.In the present invention, Paecilomyces lilacinus granule efficiently, low toxicity, insecticidal effect is stable, and to people and animals and crop safety, pacifies
Quan Xinggao.Test result indicate that, the effect that Paecilomyces lilacinus prevent and treat nematicide reaches as high as more than 78%.
Biological deposits explanation
Paecilomyces lilacinus (Paecilomyces lilacinus Samson), are deposited in Chinese microorganism strain preservation pipe
Reason committee common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and Chinese Academy of Sciences's microorganism is ground
Study carefully institute, it is CGMCC No.7994 that biological deposits are numbered.
Specific embodiment
The invention provides a kind of Paecilomyces lilacinus granule, including the component of following parts by weight：Medicine activity component 5
～15 parts, described medicine activity component is Paecilomyces lilacinus；10～25 parts of adjuvant；50～90 parts of pharmaceutical carrier；Water 15～
25 parts.
The Paecilomyces lilacinus granule that the present invention provides preferably includes 5～15 parts of Paecilomyces lilacinus, and more preferably 8～12
Part, most preferably 10 parts.
In the present invention, described Paecilomyces lilacinus are preferably selected from biological deposits and number being the strain of CGMCC No.7994
Activation culture obtains.
In the present invention, the preparation method of described Paecilomyces lilacinus comprises the following steps：
1) Paecilomyces lilacinus strain is activated；
2) Paecilomyces lilacinus after described activation are inoculated in paecilomyces lilacinus fluid medium and carry out liquid culture,
Culture 55～75h stops fermentation, obtains Paecilomyces lilacinus primary seed solution；
3) by described step 2) the Paecilomyces lilacinus primary seed solution that obtains is inoculated in Paecilomyces lilacinus solid medium,
Carry out solid culture, obtain Paecilomyces lilacinus.
In the present invention, the activation of Paecilomyces lilacinus strain is that the Paecilomyces lilacinus strain of preservation is inoculated in equipped with nutrition fine jade
In the eggplant type bottle of fat culture medium, 25～30 DEG C of culture 10～14h carry out activation culture.
After described activation, the Paecilomyces lilacinus after described activation are inoculated in fluid medium and carry out liquid training by the present invention
Support.In the present invention, the inoculum concentration of described liquid is preferably 10～30%, more preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus fluid medium preferably includes following components：Sucrose 20～40g/L, yellow
Semen Glycines powder 1.0～10g/L, ammonium nitrate 1.0～2.0g/L, disodium hydrogen phosphate 1.5～3.0g/L, magnesium sulfate 0.15～0.32g/L, chlorine
Change potassium 0.1～1.0g/L, iron sulfate 0.01～0.10g/L；More preferably sucrose 25～35g/L, analysis for soybean powder 3.0～7g/L, nitre
Sour ammonium 1.3～1.7g/L, disodium hydrogen phosphate 1.8～2.4g/L, magnesium sulfate 0.20～0.28g/L, potassium chloride 0.3～0.7g/L,
Iron sulfate 0.03～0.07g/L；Most preferably sucrose 30g/L, analysis for soybean powder 5g/L, ammonium nitrate 1.5g/L, disodium hydrogen phosphate
2.06g/L, magnesium sulfate 0.24g/L, potassium chloride 0.5g/L, iron sulfate 0.05g/L.
In the present invention, described Paecilomyces lilacinus fluid medium preferably makes after 120～130 DEG C of sterilizing 30～45min
With.In the present invention, more preferably 125 DEG C of described sterilising temp；Described sterilization time is more preferably 40min.The present invention is to institute
The equipment stating sterilizing is not particularly limited, using sterilizing installation well known to those skilled in the art, such as high pressure steam sterilization
Pot.
The present invention does not have special restriction to the equipment of described liquid culture, using culture device well known in the art
, such as fermentation tank.
In the present invention, the temperature of described Paecilomyces lilacinus liquid culture is preferably 25～30 DEG C, more preferably 26～
29 DEG C, most preferably 28 DEG C；The pH value of described Paecilomyces lilacinus liquid culture be preferably 6.6～7.2, more preferably 6.8～
7.0, most preferably 6.9；The ambient pressure of described Paecilomyces lilacinus liquid culture is preferably 0.05～0.1MPa, more preferably
0.07～0.08MpPa；The present invention is ventilated in described Paecilomyces lilacinus liquid culture, described ventilation be preferably 0.8～
1.2V/V min, more preferably 1V/V min；The time of described Paecilomyces lilacinus liquid culture is preferably 55～75h, more excellent
Elect 60～70h, more preferably 65h as.
After obtaining Paecilomyces lilacinus primary seed solution, described Paecilomyces lilacinus primary seed solution is inoculated in pale purple by the present invention
In Paecilomyces varioti solid medium, carry out solid culture, obtain Paecilomyces lilacinus.In the present invention, the inoculation of described solid culture
Amount is preferably 10～30%, more preferably 15～25%, most preferably 20%.
In the present invention, described Paecilomyces lilacinus solid medium preferably includes the component of following weight portion：Testa Tritici 60～
100 parts, 5～15 parts of Semen Maydis powder, 5～15 parts of rice husk；More preferably 70～90 parts of Testa Tritici, 7～12 parts of Semen Maydis powder, rice husk 7～12
Part；Most preferably 80 parts of Testa Tritici, 10 parts of Semen Maydis powder, 10 parts of rice husk.The present invention is preferably in described Paecilomyces lilacinus solid medium
Middle interpolation distilled water, makes solid medium water content be preferably 45%～55%, more preferably 47%～52%, most preferably
50%, then carry out solid culture.The present invention does not have special restriction to the distilled water of described interpolation, using people in the art
The conventional use of distilled water of member.
In the present invention, the temperature of described Paecilomyces lilacinus solid culture is preferably 24～30 DEG C, more preferably 26～
29 DEG C, most preferably 28 DEG C；The time of described Paecilomyces lilacinus solid culture is preferably 5～10d, more preferably 6～9d, optimum
Elect 7d as；First three sky of humidity of described Paecilomyces lilacinus solid culture is preferably 60%～90%, and remaining time is preferably 10%
～40%；More preferably 70%～85%, 15%～35%；Most preferably 80%, 30%.
After completing described Paecilomyces lilacinus solid culture, the present invention preferably will obtain solid medium to be dried, pulverizes and mistake
Sieve, obtains Paecilomyces lilacinus.In the present invention, the temperature of described drying is preferably 30～50 DEG C, more preferably 35～45 DEG C,
It is preferably 40 DEG C；The time of described drying is preferably 20～45h, more preferably 30～40h, most preferably 35h.The present invention will consolidate
Body culture medium is dried to moisture preferably between 25～35%, and more preferably 27～32%, most preferably 30%.The present invention
The method that described solid medium is pulverized does not have special restriction, using breaking method well known to those skilled in the art is
Can.In the present invention, described Paecilomyces lilacinus sieve and preferably employ 80～100 mesh, more preferably 85～95 mesh, and most preferably 90
Mesh.
The Paecilomyces lilacinus granule that the present invention provides preferably includes 10～25 parts of adjuvant, more preferably 12～20 parts,
Most preferably 15 parts.The present invention does not have special restriction to the species of described adjuvant and source, using people in the art
The commercial goods of the adjuvant of granular pesticide known to member.Specifically preferable one of glucose and corn starch
Or two kinds.In the present invention, described adjuvant specifically preferable 7～15 parts of glucoses and 3～10 parts of corn starchs, more preferably
For 8～12 parts of glucoses and 4～8 parts of corn starchs, most preferably 10 parts glucoses and 5 parts of corn starchs.In the present invention, institute
The adjuvant stated makes medicine character stable, improves drug effect.
The Paecilomyces lilacinus granule that the present invention provides preferably includes 50～90 parts of pharmaceutical carriers, and more preferably 65～80
Part, most preferably 75 parts.In the present invention, described pharmaceutical carrier is preferably kieselguhr, Pulvis Talci, white carbon, sodium sulfate, potter's clay
One or more of with Kaolin.The present invention does not have special restriction to the source of described pharmaceutical carrier, using commercially available business
Product.In the present invention, described pharmaceutical carrier is preferably specifically arbitrarily several in above-mentioned substance, can mix arbitrary proportion
Close.In the present invention, described pharmaceutical carrier is specifically more preferably Pulvis Talci and the Kaolin in above-mentioned substance, specifically can be excellent
Elect 5～15 parts of Pulvis Talci and 45～75 parts of Kaolin, more preferably 8～12 parts Pulvis Talci and 60～68 parts of Kaolin as, optimum
Elect 10 parts of Pulvis Talci and 65 parts of Kaolin as.Pharmaceutical carrier of the present invention has good medicine stability, also can improve
The drug effect of medicine.
In the present invention, described Paecilomyces lilacinus granule preferably includes 15～25 parts of water, more preferably 19～23 parts,
It is preferably 22 parts.
The invention provides the preparation method of Paecilomyces lilacinus granule described in technique scheme, comprise the following steps：
1) pharmaceutical carrier, medicine activity component and adjuvant mixed, pulverize, obtain powder；
2) by described step 1) powder that obtains mixed with water, pelletize, obtains Paecilomyces lilacinus granule.
In the present invention, the mixing of described pharmaceutical carrier, medicine activity component and adjuvant is preferably pharmaceutical carrier, medicine
Thing active component and adjuvant are divided into 3～5 parts respectively, more preferably 4 parts, and the 1st part of pharmaceutical carrier and the 1st part of medicine are lived
Property component mixing after, then mix with the 1st part of adjuvant, then mix with the 2nd part of pharmaceutical carrier again, then with the 2nd part of pharmaceutically active
Component mixes, then mixes with the 2nd part of adjuvant, by that analogy, until having added all of raw material, it is further continued for preferably mixing 40～
80min, more preferably 50～70min, most preferably 60min.The present invention does not have special restriction to the equipment of described mixing, adopts
With mixing apparatus well known to those skilled in the art, such as mixing filling.
In the present invention, 10～25 μm are preferably to the granularity of described powder, more preferably 12～20 μm, most preferably 15
μm.The present invention does not have special restriction to the condition of described pulverizing, using pulverization conditions well known to those skilled in the art.
In the present invention, described pulverizing is specifically preferably pulverized for air-flowing type, and the sealing pressure that air-flowing type is pulverized is preferably 0.05
～0.20MPa, more preferably 0.1～0.15MPa, most preferably 0.13MPa；The discharging sealing gland pressure that air-flowing type is pulverized is preferably
0.1～0.2MPa, more preferably 0.15MPa；The pulsating pressure that air-flowing type is pulverized preferably 0.3～0.7MPa, more preferably 0.4
～0.6MPa, most preferably 0.5MPa；The feed pressure of air-flowing type preferably 0.7～0.9MPa, more preferably 0.75～
0.85MPa, most preferably 0.8MPa.The present invention does not have special restriction to the equipment of described pulverizing, using people in the art
Disintegrating apparatus known to member.
After obtaining powder, the present invention, by described powder and water mixing granulation, obtains Paecilomyces lilacinus granule.In the present invention
In, the temperature of described pelletize is preferably 50～70 DEG C, more preferably 55～65 DEG C, most preferably 60 DEG C.Described Paecilomyces lilacinus
The granularity of granule is preferably 400 μm～2200 μm, more preferably 500 μm～2100 μm, most preferably 600 μm～2000 μm.This
Invention does not have special restriction to the equipment of described pelletize, using Granulation Equipments well known to those skilled in the art.
In the present invention, effective spore amount of described Paecilomyces lilacinus granule is preferably 1.8～2.2 × 108cfu/
G, more preferably 1.9～2.1 × 108Cfu/g, most preferably 2.0 × 108cfu/g.
In the present invention, miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus granule；Described Paecilomyces lilacinus de-
Fall rate≤3%.
Present invention also offers the Paecilomyces lilacinus granule described in technique scheme or side described in technique scheme
Application in terms of crops prevent and treat nematicide for the Paecilomyces lilacinus granule that method prepares.Specifically, applicable crops
Species includes cereal crops, industrial crops, vegetable crop, orchard crop, forage crop etc..Paecilomyces lilacinus of the present invention
Granule can prevent and treat each class pest and evil mite, including Meloidogyne incognita, cyst roundworm, white ball cyst roundworm, puncture nematicide,
Hairworm, golden nematode, Margarita nematicide, aphid, wood louse etc..
The present invention is not particularly limited to the application method of described Paecilomyces lilacinus granule, using people in the art
Application method known to member.
Below in conjunction with the embodiment in the present invention, the technical scheme in the present invention is clearly and completely described.Aobvious
So, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the reality in the present invention
Apply example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all belong to
In the scope of protection of the invention.
Embodiment 1
The preparation of Paecilomyces lilacinus：The Paecilomyces lilacinus strain of preservation is inoculated in the eggplant type equipped with nutrient agar
In bottle, 28 DEG C of culture 12h carry out activation culture；Paecilomyces lilacinus after activation culture are inoculated in 121 DEG C with 20% inoculum concentration
In fluid medium after sterilizing 35min, fermentation tank carries out liquid culture, the condition of concrete culture is：Temperature is 28 DEG C；
PH value is 6.9；Pressure inside the tank is 0.08MPa, and culture ventilation is 1V/V min；Stop culture after 65h, obtain pale purple plan blue or green
Mould primary seed solution；The Paecilomyces lilacinus obtaining primary seed solution is inoculated in solid medium with 20% inoculum concentration,
Add distilled water in solid medium, make solid medium water content be 50%, then carry out solid culture, condition of culture is：
Cultivation temperature is 28 DEG C, and culture first three sky of humidity is 80%, and culture humidity is 30% within latter four days, and incubation time is 7 days；Solid is trained
Support, after terminating, the Paecilomyces lilacinus of the solid culture obtaining are dried 35h under the conditions of 40 DEG C, dry pale purple to solid culture
The moisture of Paecilomyces varioti is 30%, pulverized 90 mesh sieves, and obtained Paecilomyces lilacinus.
Embodiment 2
The preparation of Paecilomyces lilacinus granule：In terms of parts by weight, by 10 parts of Pulvis Talci, 65 parts of Kaolin, 10 parts of enforcements
The Paecilomyces lilacinus of example 1 preparation, 10 parts of glucoses and 5 parts of corn starchs are divided into 4 heaps, respectively by the 1st heap Pulvis Talci and the 1st
After the mixing of heap Kaolin, then mix with the 1st heap Paecilomyces lilacinus, then mix with the 1st heap glucose, then with the 1st heap corn starch
Mixing, then mix with the 2nd heap Pulvis Talci, by that analogy, until all raw materials have all mixed, it is further continued for mixing 60min, obtains
Compound；Compound is pulverized through air-flowing type, obtains 15 μm of powder；Powder adds 22 parts of water again, under the conditions of temperature 60 C,
It is manufactured into 1200 μm of granule, obtain Paecilomyces lilacinus granule.
The quality control index of the Paecilomyces lilacinus granule of table 1 embodiment 2 preparation
Embodiment 3
The preparation of Paecilomyces lilacinus granule：In terms of parts by weight, by 75 parts of Kaolin, 10 parts of embodiments 1 prepare light
Purple Paecilomyces varioti, 15 parts of glucoses are divided into 4 heaps respectively, after the 1st heap Kaolin is mixed with the 1st heap Paecilomyces lilacinus, then with
1st heap glucose mixing, then mix with the 2nd heap Kaolin, by that analogy, until all raw materials have all mixed, it is further continued for mixing
Close 50min, obtain compound；Compound is pulverized through air-flowing type, obtains 10 μm of powder；Powder adds 25 parts of water again, in temperature
Under the conditions of 60 DEG C of degree, it is manufactured into 1600 μm of granule, obtains Paecilomyces lilacinus granule.
Embodiment 4
The preparation of Paecilomyces lilacinus granule：In terms of parts by weight, by 65 parts of Kaolin, 10 parts of kieselguhr, 15 parts of enforcements
The Paecilomyces lilacinus of example 1 preparation, 7 parts of glucoses and 3 parts of corn starchs are divided into 4 heaps, respectively by the 1st heap Kaolin and first
After the mixing of heap kieselguhr, then mix with the 1st heap Paecilomyces lilacinus, then mix with the 1st heap glucose, then with the first heap corn starch
Mixing, then mix with the 2nd heap Kaolin, by that analogy, until all raw materials have all mixed, it is further continued for mixing 40min, obtains
Compound；Compound is being pulverized through air-flowing type, obtains 12 μm of powder；Powder adds 20 parts of water again, in temperature 50 C condition
Under, it is manufactured into 1000 μm of granule, obtain Paecilomyces lilacinus granule.
Embodiment 5
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is Hylocereus undatuss, and management is good, and root-knot nematode occurs
Medium weighting.Experimental field is divided into four regions by this test, and region 1 is matched group, only along ridge spray clear water；Region 2 is suitable
Ridge sprays 1000 times of diluents of Paecilomyces lilacinus of preparation in embodiment 2；Region 3 sprays the 1000 times of dilutions of 3% Furadan along ridge
Liquid, the concentration of medicine activity component is identical with region 2；Region 4 sprays 1000 times of diluents of 90% trichlorphon, medicine along ridge
The concentration of active component is identical with region 2 and 3；The dosage in four regions is identical, 1 day after dispenser, 3 days, and 5 days, each investigation in 7 days
Once borer population alive, calculates Revision insect recluced rate.Concrete outcome as shown in table 2, can show, the insect population in region 2 subtracts by the data of table 2
Move back rate highest, show the insecticidal effect of Paecilomyces lilacinus granule of present invention preparation preferably, its drug effect is above same concentration
Metrifonate and Furadan.
Parasite killing (root-knot nematode) effect of table 2 Paecilomyces lilacinus granule
Can draw from the test data of table 2, the Paecilomyces lilacinus granule Revision insect recluced rate of 7 days of present invention preparation can
Up to 78.6%, its insecticidal effect is higher than Furadan and metrifonate.
Embodiment 5
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is Citrus, and management is good, punctures in nematicide generation
Deng weighting.Experimental field is divided into four regions by this test, and region 1 is matched group, only along ridge spray clear water；Region 2 is along ridge
Spray 2000 times of diluents of Paecilomyces lilacinus granule of preparation in embodiment 2；Region 3 sprays 1000 times of 3% Furadan along ridge
Diluent, the concentration of medicine activity component is identical with region 2；Region 4 sprays 1000 times of diluents of 90% trichlorphon along ridge,
The concentration of medicine activity component is identical with region 2 and 3；The dosage in four regions is identical, and 1 day after dispenser, 3 days, 5 days, 7 days each
Investigation once borer population alive, calculates Revision insect recluced rate.Concrete outcome as shown in table 3, can be drawn by the data of table 3, the worm in region 2
Mouth decline rate highest, shows the insecticidal effect of Paecilomyces lilacinus granule of present invention preparation preferably, its drug effect is higher than with dense
The Furadan of degree, significantly larger than with the metrifonate of concentration.
Parasite killing (the puncturing nematicide) effect of table 3 Paecilomyces lilacinus granule
Can draw from the test data of table 3, the Paecilomyces lilacinus granule Revision insect recluced rate of 7 days of present invention preparation can
Up to 78.2%, its insecticidal effect is higher than the Furadan of same concentration, significantly larger than with the metrifonate of concentration.
As seen from the above embodiment, the Paecilomyces lilacinus granule of present invention preparation, may be up to the control rate of nematicide
More than 78%, insecticidal effect is higher than Furadan and metrifonate.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Paecilomyces lilacinus granule is it is characterised in that include the component of following parts by weight：
5～15 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
10～25 parts of adjuvant；
50～90 parts of pharmaceutical carrier；
15～25 parts of water.
2. a kind of Paecilomyces lilacinus granule according to claim 1 is it is characterised in that described Paecilomyces lilacinus are selected from life
Thing deposit number is the strain of CGMCC No.7994.
3. a kind of Paecilomyces lilacinus granule according to claim 1 is it is characterised in that described Paecilomyces lilacinus granule
Effective spore amount of agent is 1.8～2.2 × 108cfu/g.
4. a kind of Paecilomyces lilacinus granule according to claim 1 or 3 is it is characterised in that described Paecilomyces lilacinus
Miscellaneous bacteria rate≤0.1% of granule.
5. a kind of Paecilomyces lilacinus granule according to claim 1 is it is characterised in that described Paecilomyces lilacinus granule
Granularity be 400 μm～2200 μm.
6. a kind of Paecilomyces lilacinus granule according to claim 1 it is characterised in that described Paecilomyces lilacinus de-
Fall rate≤3%.
7. Paecilomyces lilacinus granule according to claim 1 it is characterised in that described adjuvant include glucose and/
Or corn starch.
8. Paecilomyces lilacinus granule according to claim 1 it is characterised in that described pharmaceutical carrier include kieselguhr,
One or more of Pulvis Talci, white carbon, sodium sulfate, potter's clay and Kaolin.
9. the Paecilomyces lilacinus granule described in claim 1～8 any one preparation method it is characterised in that include with
Lower step：
1) pharmaceutical carrier, medicine activity component and adjuvant mixed, pulverize, obtain powder；
2) by described step 1) powder that obtains mixed with water, pelletize, obtains Paecilomyces lilacinus granule.
10. application in terms of preventing and treating crops nematicide for the Paecilomyces lilacinus granule described in claim 1～8 any one.
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Cited By (3)
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| CN106929457A (en) * | 2017-05-10 | 2017-07-07 | 吉安职业技术学院 | A kind of Ke Liben series bacillus granule and preparation method thereof |
| CN112111480A (en) * | 2020-10-14 | 2020-12-22 | 南宁汉和生物科技股份有限公司 | Method for prolonging shelf life of paecilomyces lilacinus by using double-layer embedding technology |
| CN114176096A (en) * | 2021-11-10 | 2022-03-15 | 河北兴柏农业科技有限公司 | Streptomyces avermitilis active spore pesticide and preparation method thereof |
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| CN106929457A (en) * | 2017-05-10 | 2017-07-07 | 吉安职业技术学院 | A kind of Ke Liben series bacillus granule and preparation method thereof |
| CN112111480A (en) * | 2020-10-14 | 2020-12-22 | 南宁汉和生物科技股份有限公司 | Method for prolonging shelf life of paecilomyces lilacinus by using double-layer embedding technology |
| CN114176096A (en) * | 2021-11-10 | 2022-03-15 | 河北兴柏农业科技有限公司 | Streptomyces avermitilis active spore pesticide and preparation method thereof |
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