CN104770397B - A kind of muscardine pulvis and its application - Google Patents
A kind of muscardine pulvis and its application Download PDFInfo
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- CN104770397B CN104770397B CN201510035944.7A CN201510035944A CN104770397B CN 104770397 B CN104770397 B CN 104770397B CN 201510035944 A CN201510035944 A CN 201510035944A CN 104770397 B CN104770397 B CN 104770397B
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- muscardine
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- beauveria bassiana
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Abstract
The invention provides a kind of muscardine pulvis, include by weight percentage：Beauveria bassiana spore powder 1~5%, ultraviolet resistance protectant 0.5~2%, filler 94%~98%.After active material beauveria bassiana spore powder is mixed with filler, uvioresistant auxiliary agent is added, is stirred in dry powder blender, dispensed, produce uvioresistant muscardine powder product.Additionally provide the application that muscardine pulvis is used to prevent and treat corn borer in maize straw.Preventing and treating of the product of the present invention to corn borer in maize straw has excellent effect, also strengthens with pollution-free, adhesion strength, the characteristics of resistance of rainwater washing against.
Description
Technical field
The invention belongs to biological pesticide technical field, and in particular to a kind of muscardine pulvis and its application.
Background technology
Muscardine is a kind of fungal microbe insecticide, and its mode of action is secretion after beauveria bassiana spore contact insect
Various insects skin degradation enzyme, penetrate insect body wall and enter body cavity, bred rapidly in polypide；A large amount of muscardine poison are secreted simultaneously
Element, destroys the body tissue of insect, finally makes insect dead because that can not maintain normal vital movement.Because muscardine has
Selectivity is strong, and to pest natural enemy safety, insecticidal mechanism is unique, is not likely to produce the resistance to the action of a drug, the characteristic such as green, at present should turn into
With one of most commonly used fungus insecticide.In China, beauveria bassiana has been widely used in dendrolimus punctatus
A variety of forestry and the preventing and treating of Agriculture field pests such as (Dendrolimus punctatus Walker) and corn borer, and achieve significantly
Economy, society and ecological benefits.
Ostrinia furnacalis (Ostrinia furnacalis Guen é e, hereinafter referred to as corn borer), " corn infiltrates alias
Worm ", it is that a kind of maximum insect is endangered corn in Maize Production.Have in various degree in the area of Jilin Province's plantation spring maize
Occur.It is general the time occurs caused by production loss the production loss caused by 10% or so, outbreak year part 30% with
On.Corn borer brill moth corn stem, female fringe, gnaw corn lobus cardiacus, make aggrieved some lost function, not only influence the production of corn
Amount, it can also cause secondary disease, producing toxic metabolite influences the quality of corn, therefore corn borer is always Jilin Province for many years
One of agricultural pests of keypoint control.Corn borer is complete metamorphosis, and its all one's life will pass through " ovum --- larva ---
The harm of the four-stages such as pupa --- adult ", wherein larval stage to crops is the most serious.Corn borer is since ovum to adult
This ontogeny cycle of sexal maturity is called a generation, an abbreviation generation, in 1 year 1~2 generation of main generation of Jilin Province maize snout moth's larva.
Overwintering larva develops into adult after pupating, adult lays eggs in corn growing season corn leaf back, and egg stage of Ostrinia furnacalis develops into larva
Afterwards, just start to endanger plant.The corn borer that two generations occurred can complete a generation in summer, and no matter a generation or two generations occur
Corn borer larvae all survived the winter in maize straw.Thus, maize straw is the insects sources that next year 1st generation corn borer occurs.
One of corn cereal crops main as China, general chemistry medicament are difficult to eliminate the corn passed the winter in stalk
Snout moth's larva larva, a kind of effectively preventing method is needed badly to reduce corn borer population radix, what the corn borer harm of reduction next year occurred can
Energy.
The content of the invention
It is an object of the invention to provide a kind of beauveria bassiana spore preparation for preventing and treating Ostrinia furnacalis, north is particularly suitable for
Fang Chunji reduces uvioresistant beauveria fungus preparation of corn borer population radix and its preparation method and application.
The present invention realizes especially by following technical scheme：
A kind of muscardine pulvis, includes by weight percentage：Beauveria bassiana spore powder 1~5%, ultraviolet resistance protectant
0.5~2%, filler 94%~98%.
Preferably, a kind of muscardine pulvis, includes by weight percentage：Beauveria bassiana spore powder 3%, uvioresistant protection
Agent 1%, filler 96%.
Described muscardine is beauveria bassiana DSXJ-07, and the bacterial strain has carried out preservation, depositary institution：China Microbiological
Culture presevation administration committee common micro-organisms center；Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Preservation date：
On 01 23rd, 2013；Deposit number：CGMCC No.7192；Described beauveria bassiana DSXJ-07 the Internal Transcribed Spacer
Sequence is SEQ ID No.1.
One or more of the described filler in talcum powder, diatomite, concave convex rod or kaolin, preferably talcum
Powder.
It is one or more of that described ultraviolet resistance protectant is selected from titanium dioxide, ascorbic acid, zinc oxide.
Every gram of pulvis lives spore not less than 3,000,000,000 containing muscardine in described muscardine pulvis, and spore germination rate >=
90%, water content control is below 10%.
After muscardine pulvis of the present invention is by the way that active material beauveria bassiana spore powder is mixed with filler, uvioresistant is added
Auxiliary agent, stirred in dry powder blender, dispense, produce uvioresistant muscardine powder product.
Muscardine pulvis of the present invention is used for the application for preventing and treating corn borer in maize straw, is completed especially by following methods：
A. corn pile is carried out before pupating in corn borer spring to cut open bar investigation；
B. by muscardine pulvis, load in power-driven powder-spraying machine, it is stand-by；
C. by the discharge tube insertion maize straw pile of duster, start duster 3-5s or so and dust, every cubic metre 1
Point, untill full pile has sprayed；
D. whole stalk pile is all around sprayed one time thoroughly, comprehensively with power-driven powder-spraying machine.
When investigation standard reaches 50 for the live body corn borer larvae quantity in every 100 plants of corn stalks in step (a), i.e.,
It is defined as the index for control that corn borer seal pile preventing and treating.
The dosage of described muscardine pulvis is that every cubic metre of maize straw pile is sprayed for 100 grams with muscardine pulvis
Apply.
Muscardine pulvis technical indicator of the present invention：
Beneficial effects of the present invention are：1) focus on its biocompatibility with muscardine in auxiliary agent selection, select auxiliary agent
Muscardine conidia germination and mycelia day growth amount and production spore are not influenceed or influenceed smaller；2) product performance index is excellent
It is good；3) present invention is muscardine pulvis, and the formulation adds ultraviolet protective agent, beauveria bassiana spore is influenceed very by ultraviolet
It is small, the life-span of beauveria bassiana spore is extended, improves the prevention effect of muscardine pulvis, 4) reduce pesticide usage amount, reduce
Residual quantity of the agricultural chemicals on crop, alleviates environmental pollution, and new material is provided to carry out nuisanceless agricultural preventing and treating；5) to people
Poultry safety, Environmental compatibility are good；And preparation adhesion strength strengthens, resistance of rainwater washing against.
Brief description of the drawings
Fig. 1 is the germination rate of different filler beauveria bassiana conidial powders；
Fig. 2 be different ultraviolet resistance protectants muscardine pulvis under ultraviolet light beauveria bassiana spore germination rate.
Embodiment
With reference to embodiment, the present invention is described further, as described below, is only the preferable implementation to the present invention
Example, is not limited the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents be changed to the equivalent embodiment changed on an equal basis.It is every of the invention without departing from the present invention program content, foundation
Technical spirit any simple modification that following examples are made or equivalent variations, all fall within protection scope of the present invention.
Following technical scheme is unless otherwise specified, technological means used in embodiment is well known to those skilled in the art
Conventional meanses.
Muscardine pulvis consists of：Beauveria bassiana spore powder (female powder) 1~5%, ultraviolet resistance protectant 0.5~2%,
Filler 94%~98%.
1) screening of filler
By beauveria bassiana conidial powder with supplying examination filler (talcum powder, diatomite, attapulgite, kaolin) 1:1(W/
W) ratio uniform mixes, and is placed in low-temperature cold store (3 ± 1) DEG C, under the conditions of RH=75%, dark storage, monthly takes quantitative mixing
Powder determines spore germination rate, with the same terms in spore germination liquid after (25 ± 1) DEG C, 120r/min concussion and cultivates 24h
The pure conidia powder of storage is compared and (3 samples of measure every time, determined 5 months altogether).
When mixing powder is stored 1 month, spore germination rate influences that more than 80% level less, can be maintained substantially,
But with the extension of period of storage, spore germination rate is gradually reduced, when extremely storing 5 months, attapulgite, kaolin, diatomite
The spore germination rate of mixture has dropped to 50%, and the only mixture spore germination rate of talcum powder and control declines without too big
Change, more than 80% level can be maintained, and the spore germination rate of talcum powder mixture can also reach 85.54%.
Storage test result shows：Talcum powder is the optimal filler of muscardine pulvis, sees Fig. 1.
2) screening of ultraviolet resistance protectant
It is different to add the muscardine pulvis of the different ultraviolet resistance protectants beauveria bassiana spore germination rate under ultraviolet light,
Little to the Spore Germination of muscardine in ultraviolet light 4h, beauveria bassiana spore germination rate is above 90%；Ultraviolet
During light irradiation 8h, beauveria bassiana spore germination rate starts change substantially, adds the muscardine pulvis germination rate of protective agent titanium dioxide
More than 80% can also be reached, and add other 2 kinds protectant beauveria bassiana spore germination rates and decline substantially, just reach 70%；With
The extension (during ultraviolet light 14h) of irradiation time, the muscardine pulvis germination rate for adding protective agent titanium dioxide still reaches
To 80%, and add other 2 kinds (ascorbic acid, zinc oxide) protectant beauveria bassiana spore germination rate and decline obvious quickening,
Spore germination rate is less than 60% during ultraviolet light 10h, in ultraviolet light 14h, spore germination rate below 40%,
See Fig. 2.Therefore, ultraviolet protective agent titanium dioxide is obvious to protecting the sprouting of beauveria bassiana spore to act on.
After the muscardine pulvis mixes active material beauveria bassiana spore powder with filler, uvioresistant auxiliary agent is added,
Stirred in dry powder blender, dispense, produce uvioresistant muscardine powder product.
Embodiment 1
A kind of muscardine pulvis, it is composed of the following components by weight percentage：Beauveria bassiana spore powder 1%, titanium dioxide
2%th, talcum powder 97%.
Embodiment 2
A kind of muscardine pulvis, it is composed of the following components by weight percentage：Beauveria bassiana spore powder 1.5%, Vitamin C
Acid 0.5%, diatomite 98%.
Embodiment 3
A kind of muscardine pulvis, it is composed of the following components by weight percentage：Beauveria bassiana spore powder 3%, zinc oxide
1%th, kaolin 96%.
Embodiment 4
A kind of muscardine pulvis, it is composed of the following components by weight percentage：Beauveria bassiana spore powder 2.5%, concave convex rod
With kaolin 1.5%, titanium dioxide and ascorbic acid 96%.
The prevention effect of knot and specific description of test muscardine pulvis of the present invention to corn borer in maize straw below.
Embodiment 5
Test period：15 to 20 April in 2013
Test site：City of Gongzhuling of Jilin Province
Test material：
Preparation：The scheme of embodiment 1~4 prepares muscardine pulvis；Character：White powder, no special odor, effective content 25
~30 hundred million spores/gram.
Instrument：Power knapsack duster one.
Test method：Representational 5 maize straw piles are chosen in the black woods town difference village village in city of Gongzhuling of Jilin Province
As subjects, muscardine pulvis is loaded in power-driven powder-spraying machine, by the discharge tube insertion hole of duster, startup is dusted
Machine, 3-5s is sprayed, then spray second (1 point/m of position3), so repeat untill full pile has sprayed.Conscientiously check and sprayed in hole
Uniformity coefficient, finally by whole stalk pile all around with power-driven powder-spraying machine again thoroughly, comprehensively sprinkling one time.
Corn borer larvae bombys batryticatus rate investigation in maize straw pile is carried out in 1 month after preventing and treating of dusting.Using random searching
Method, representational different check plots and treatment region are selected, record corn borer larvae in every 100 plants of maize straws respectively and live
Borer population, dead borer population, bombys batryticatus number, other dead borer population (bacterium, honeybee are parasitic and fly is parasitic), pupa numbers (being shown in Table 1) living.In system after autumn
Investigate corn field plant Harm (being shown in Table 2) around the stalk pile that dusts.
Table 1：Muscardine pulvis sprays corn borer larvae bombys batryticatus rate application form after stalk pile
As can be seen from Table 1：After muscardine pulvis sprays maize straw pile, in pile corn borer larvae bombys batryticatus rate it is obvious on
Rise, exceeded 86.00%, significantly larger than the 16.67% of check plot, using corn borer children in muscardine pulvis each group corn pile
Worm is remarkably reinforced by muscardine parasitism, and corn borer larvae bombys batryticatus rate is up to more than 86%.It is easy easily that corn pile is sprayed using muscardine
In popularization, corn borer population radix can be efficiently controlled, the harm of corn borer is controlled from source.
As can be seen from Table 2：The killed strain in field, channel number, borer population living are compareed apparently higher than each processing field；Each processing field quilt
Evil strain decline rate is above 50%, and the killed strain decline rate for handling four reaches 70.14%；Each processing field worm channel decline rate exceedes
60%, the worm channel decline rate for handling five reaches 75.29%；Each processing field Revision insect recluced rate has exceeded 60%, handles four
Revision insect recluced rate minimum 64.71%, three Revision insect recluced rate highest is handled, reaches 81.37%；It is super that equal preventive effect is patted everywhere
Cross 65%.
Table 2：Muscardine pulvis sprays corn field corn borer harm nearby after maize straw pile a situation arises application form
The technical perfection has simultaneously innovated Beauveria Bassiana Powder agent prescription, and ultraviolet protective agent is added in formula, extends muscardine
The spore life-span, and improve prevention effect.The advantages of technology, is that simple to operate, preventive effect is good, in pile bombys batryticatus rate up to 85% with
On, field control effect is up to more than 65%.The technology had both overcome traditional muscardine pulvis and has sprayed maize straw pile preventing and treating jade
The shortcomings that rice snout moth's larva preventive effect is low；Solve and taken a lot of work problem that is laborious, can not being widely applied using Control of The European Corn Borer With Granulated Insecticides again, tool
There is very wide popularizing application prospect.
Claims (6)
1. a kind of muscardine pulvis, it is characterised in that include by weight percentage：Beauveria bassiana spore powder 1~5%, uvioresistant
Protective agent 0.5~2%, filler 94%~98%；
Described muscardine is beauveria bassiana DSXJ-07, and the bacterial strain was preserved in China Microbiological on 01 23rd, 2013
Culture presevation administration committee common micro-organisms center, deposit number are CGMCC No.7192；Described beauveria bassiana
The sequence of DSXJ-07 the Internal Transcribed Spacer is SEQ ID No.1.
2. muscardine pulvis according to claim 1, it is characterised in that include by weight percentage：Beauveria bassiana spore powder
3%th, ultraviolet resistance protectant 1%, filler 96%.
3. muscardine pulvis according to claim 1 or 2, it is characterised in that：Described filler is selected from talcum powder, diatom
One or more in soil, concave convex rod or kaolin；Described ultraviolet resistance protectant is selected from titanium dioxide, ascorbic acid, oxygen
Change the one or more of zinc.
4. muscardine pulvis according to claim 1 or 2, it is characterised in that：Every gram of pulvis in described muscardine pulvis
Spore is lived not less than 3,000,000,000 containing muscardine, and spore germination rate reaches more than 90%, water content control is below 10%.
5. the muscardine pulvis described in claim 1 is used for the application for preventing and treating corn borer in maize straw.
6. application according to claim 5, it is characterised in that：The dosage of described muscardine pulvis is every cubic metre of corn
Stalk pile is sprayed for 100 grams with muscardine pulvis.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106007824B (en) * | 2016-05-23 | 2019-12-27 | 泰谷生态科技集团股份有限公司 | Composite bacterial fertilizer and preparation method and application thereof |
| CN106386866A (en) * | 2016-08-19 | 2017-02-15 | 吉林省农业科学院 | Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis |
| CN108719292B (en) * | 2018-07-07 | 2021-06-08 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Special synergist for beauveria bassiana biological pesticide and preparation method and application thereof |
| CN109769805A (en) * | 2019-03-28 | 2019-05-21 | 吉林省农业科学院 | A kind of emulsifiable pulvis of beauveria bassiana and preparation method thereof |
| CN111567564A (en) * | 2020-06-05 | 2020-08-25 | 吉林省农业科学院 | Method for preventing and treating corn borers by using plant protection unmanned aerial vehicle |
| CN115152796A (en) * | 2022-08-02 | 2022-10-11 | 福建农林大学 | Beauveria bassiana and pyrethrin composite wettable powder and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099490A (en) * | 2007-04-05 | 2008-01-09 | 吴学民 | Beauvericin insecticide suspending agent and preparation method thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101099490A (en) * | 2007-04-05 | 2008-01-09 | 吴学民 | Beauvericin insecticide suspending agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 针对小菜蛾的球孢白僵菌孢子可湿性粉剂研制的技术探索;田丽;《中国优秀博士学位论文全文数据库 工程科技I辑》;20050831;第5-14、35-38页 * |
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