CN106386866A - Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis - Google Patents
Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis Download PDFInfo
- Publication number
- CN106386866A CN106386866A CN201610695297.7A CN201610695297A CN106386866A CN 106386866 A CN106386866 A CN 106386866A CN 201610695297 A CN201610695297 A CN 201610695297A CN 106386866 A CN106386866 A CN 106386866A
- Authority
- CN
- China
- Prior art keywords
- muscardine
- hubern
- prevention
- control
- pyrausta nubilalis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention discloses a biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis. 1L of the biopesticide comprises 0.1g of beauveria bassiana, 10g of bacillus thuringiensis, 6% of an auxiliary agent 1# on the basis of a volume ratio, 2% of an auxiliary agent 2# on the basis of a volume ratio, 0.05% of xanthan gum on the basis of a volume ratio, 0.05% of formaldehyde on the basis of a volume ratio, 5% of urea on the basis of a mass-volume ratio and the balance water. Through combination of beauveria bassiana and bacillus thuringiensis, a water suspending agent is prepared. Through high rod sprayer spraying, Asiatic corn borers in the field can be prevented and controlled and an insecticidal period is shortened by 20%. Compared with a single beauveria bassiana water suspending agent with a beauveria bassiana concentration of 1*10<9> and with the same cost, the biopesticide utilizes combination of 1*10<4> spore/ml of beauveria bassiana and 1*10<7> spore/ml of bacillus thuringiensis to prevent and control corn borers, shortens an indoor insecticidal period by 20% or more and improves field prevention effects by 20% or more.
Description
Technical field
The invention belongs to Pyrausta nubilalis (Hubern). Prevention Technique field, more particularly, to a kind of anti-with bacillus thuringiensiss using muscardine
The biological pesticide of control Pyrausta nubilalis (Hubern)..
Background technology
Semen Maydiss are the second largest cereal crops of China, produce 300000000000 jin per year, can be used to edible it is also possible to as feedstuff and industry
Raw material production industrial alcohol and liquor etc..And Pyrausta nubilalis (Hubern). is the primary pest in Maize Production, not only take food maize leaf, eat into into
In Semen Maydiss stem or fruit ear, reduce photosynthesis, affect nutrient transport；Also produce various secondary diseases, cause the Semen Maydiss underproduction to be dropped
Matter, in 7%-15%, the serious time underproduction more than 50%, or even total crop failure, to China's grain for the production loss throughout the year causing Semen Maydiss
Produce and cause huge economic loss.Chemical pesticide plays main effect always in the preventing and treating of Pyrausta nubilalis (Hubern). it is ensured that grain
Volume increase and safety problem, but be as chemical pesticide a large amount of, continuous use, pesticide residues are to edible safety and Environmental security
Harm is increasingly severe, and the good highly effective pesticide of environment compatibility has become the direction of Agrochemicals.At present, China's Biological control is beautiful
Rice snout moth's larva mainly adopts Beauveria Bassiana In Corn Stalk-stubble Piles For Controlling Corn Borers and Trichogramma preventing and treating.Muscardine (Beauveria bassiana) is
A kind of filamentous fungis, are the widest insect pathogenic fungus of current domestic application.Muscardine has pathogenic strong, insecticidal range
Extensively, advantages of environment protection.But still suffer from that dosage form is single, labor efficiency is low, parasite killing speed is slow, insecticide efficiency is low in preventing and treating
Shortcoming.At present, muscardine is the preventing and treating of Pyrausta nubilalis (Hubern). and pine moth in China most successful popularization model, but is government and promotes,
Using most be muscardine powder, based on spring envelope Semen Maydiss buttress and air-dusting；Beauveria bassiana granular formulation also has use, but labor
Efficiency of movement is low, has faded away.Muscardine is caused to be that strain virulence is moved back in the main cause that China promotes and commercialization is difficult
Change, produce the reasons such as spore power is unstable and dosage form is single.Various countries add to the separation of bacillus thuringiensis bacterial strain and application in recent years
Big dynamics, and it is widely used to each Lepidopterous, coleopteran pest such as beet armyworm Spodoptera exigua (H ü
Bner), Prodenia litura S.litura (Fabricius), dendrolimus punctatus Dendrolimuspunctatus (Walker), tea chi
Earwig Ectropis obliqua (Prout), rice-stem borer Chilo suppressalis (Walker) (Li Qing etc., 2009；)、
The preventing and treating of Aedes albopictus Aedes albopictus (Zhang Lingling etc., 2008), Holotrichia diomphalia Batess etc..But it is applied to the Su Yun of Pyrausta nubilalis (Hubern). preventing and treating
The applied research of golden Bacillus strain is less.
At present, muscardine or bacillus thuringiensiss be used alone preventing and treating Pyrausta nubilalis (Hubern). exist parasite killing cycle length, be also easy to produce anti-
Property, relatively costly etc..
Content of the invention
It is an object of the invention to provide the biological agriculture of a kind of utilization muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
There is parasite killing cycle length, be also easy to produce in medicine it is intended to solving current muscardine or bacillus thuringiensiss and being used alone preventing and treating Pyrausta nubilalis (Hubern).
Resistance, relatively costly problem.
The present invention is achieved in that the biological agriculture of a kind of utilization muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Medicine, described utilization muscardine is high by 0.1g muscardine (D1-5 bacterial strain) with the biological pesticide of bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
(spore density is 1 × 10 to cryptogam11/ g), the high cryptogam of 10g bacillus thuringiensiss (JN001 bacterial strain) (spore density be 1 ×
1012/ g), 6% auxiliary agent 1 (volume ratio) #, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (body
Long-pending ratio), 5% carbamide (mass volume ratio), water supply 1L composition.
Further, described utilization muscardine is dilute with the aqueous suspension agent of the biological pesticide of bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Release 1000 times, beauveria bassiana spore final concentration of 1 × 104Individual spore/ml, bacillus thuringiensiss final concentration of 1 × 107Individual spore/
ml.
Another object of the present invention is to providing a kind of described utilization muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Biological pesticide preparation method, described preparation method comprises the following steps：
(spore density is 1 × 10 to measure the high cryptogam of 0.1g muscardine (D1-5 bacterial strain) respectively11/ g), 10g Su Yun gold spore
(spore density is 1 × 10 to the high cryptogam of bacillus (JN001 bacterial strain)12/ g), 6% auxiliary agent 1 (volume ratio) #, 2% auxiliary agent 2# (volume
Than), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% carbamide (mass volume ratio), be fully ground mixing
Afterwards, sterilized water is added to supply 1L, sealing preserve.
Further, the using method bag of the biological pesticide of described use muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Include：
By preparation biological pesticide aqueous suspension agent dilute 1000 times, using high bar spraying machine mid-June Semen Maydiss loudspeaker
The mouth phase carries out field spray preventing and treating, every mu of 20L.
The biological pesticide of utilization muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern). that the present invention provides, by muscardine or
Bacillus thuringiensiss combine, and formulate aqueous suspension agent, carry out Ostrinia furnacalis field control, parasite killing cycle using the spraying of high bar
Shorten 20%.Muscardine is combined prevention and control Field Pests and has had been reported that predominantly wettable powder at present with bacillus thuringiensiss
Preventing and treating tent caterpillar, liquid preparation preventing and treating distillers yeast insect grain peat etc., combine the phase of aqueous suspension agent prevention and control Pyrausta nubilalis (Hubern). using both
Close research have not been reported.Be used alone 1 × 109Under the identical cost of concentration muscardine aqueous suspension agent, apply 1 × 104Individual spore
Son/ml muscardine and 1 × 107Individual spore/ml bacillus thuringiensiss, joint prevention and control Pyrausta nubilalis (Hubern). indoor insecticidal cycle time 20%
More than, field efficacy improves more than 20%；
Field information：The aqueous suspension agent of preparation is diluted 1000 times, using high bar spraying machine in Semen Maydiss trumpet period (June
The middle ten days) carry out field spray preventing and treating, every mu of 20L；
Cut open bar investigation Revision insect recluced rate late September, using non-medication plot as comparison, muscardine and Su Yun gold spore bar
Bacterium use in conjunction preventive effect reaches 85.06%, and being used alone muscardine preventive effect is 66.67%, and preventive effect improves 21.62%.
Toxicity Determination：The aqueous suspension agent of preparation is diluted 1000 times, beauveria bassiana spore final concentration of 1 × 104Individual spore
Son/ml, bacillus thuringiensiss final concentration of 1 × 107Individual spore/ml, is carried out to Ostrinia furnacalis 2 instar larvae using infusion process
Toxicity Determination, respectively to dilute 1000 times as blank without muscardine and bacillus thuringiensis suspending agent auxiliary agent,
On the basis of identical production cost, with muscardine 1 × 109Spore/ml is comparison.Toxicity test result shows, muscardine and Soviet Union
Cloud gold bacillus cereuss aqueous suspension agent LT90 is 7.05 days, and being used alone muscardine LT90 is 8.98 days, and the parasite killing time shortens
21.49%.
Brief description
Fig. 1 is the biological pesticide of utilization muscardine provided in an embodiment of the present invention and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Preparation method flow chart.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is explained in detail.
The utilization muscardine of the embodiment of the present invention is white by 0.1g with the biological pesticide of bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
(spore density is 1 × 10 to the high cryptogam of stiff bacterium (D1-5 bacterial strain)11/ g), 10g bacillus thuringiensiss (JN001 bacterial strain) high cryptogam
(spore density is 1 × 1012/ g), 6% auxiliary agent 1 (volume ratio) #, 2% auxiliary agent 2# (volume ratio), 0.05% xanthan gum (volume
Than), 0.05% formaldehyde (volume ratio), 5% carbamide (mass volume ratio), water supply 1L composition..
As shown in figure 1, the biological agriculture of the utilization muscardine of the embodiment of the present invention and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
The preparation method of medicine comprises the following steps：
S101：(spore density is 1 × 10 to measure the high cryptogam of 0.1g muscardine (D1-5 bacterial strain) respectively11/ g), 10g Su Yun gold
(spore density is 1 × 10 to the high cryptogam of bacillus cereuss (JN001 bacterial strain)12/ g), 6% auxiliary agent 1 (volume ratio) #, 2% auxiliary agent 2# (body
Long-pending ratio), 0.05% xanthan gum (volume ratio), 0.05% formaldehyde (volume ratio), 5% carbamide (mass volume ratio)；
S102：After being fully ground mixing, sterilized water is added to supply 1L, sealing preserve.
With reference to specific embodiment, the application effect of the present invention is explained in detail.
Embodiment 1：
Toxicity Determination：The aqueous suspension agent of preparation is diluted 1000 times, beauveria bassiana spore final concentration of 1 × 104Individual spore
Son/ml, bacillus thuringiensiss final concentration of 1 × 107Individual spore/ml, is carried out to Ostrinia furnacalis 2 instar larvae using infusion process
Toxicity Determination, respectively to dilute 1000 times as blank without muscardine and bacillus thuringiensis suspending agent auxiliary agent,
On the basis of identical production cost, with muscardine 1 × 109Spore/ml is comparison.Toxicity test result shows, muscardine and Soviet Union
Cloud gold bacillus cereuss aqueous suspension agent LT90 is 7.05 days, and being used alone muscardine LT90 is 8.98 days, and the parasite killing time shortens
21.49%.
Embodiment 2：
Field information：The aqueous suspension agent of preparation is diluted 1000 times, using high bar spraying machine in Semen Maydiss trumpet period (June
The middle ten days) carry out field spray preventing and treating, every mu of 20L.Cut open bar investigation Revision insect recluced rate late September, using non-medication plot as right
According to muscardine and bacillus thuringiensiss use in conjunction preventive effect reach 85.06%, and being used alone muscardine preventive effect is 66.67%, prevents
Effect raising 21.62%.
Make detailed description with reference to the application effect comparing the present invention.
Be used alone 1 × 109Under the identical cost of concentration muscardine aqueous suspension agent, apply 1 × 104Individual spore/ml Bombyx Batryticatus
Bacterium and 1 × 107Individual spore/ml bacillus thuringiensiss, joint prevention and control Pyrausta nubilalis (Hubern). indoor insecticidal cycle time more than 20%, field
Preventive effect improves more than 20%.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (4)
1. the biological pesticide of a kind of utilization muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern). is it is characterised in that described utilization
The biological pesticide of muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern). by 0.1g muscardine, 10g bacillus thuringiensiss, 6%
Auxiliary agent 1#, 2% auxiliary agent 2#, 0.05% xanthan gum, 0.05% formaldehyde, 5% carbamide, water composition；
Wherein, 6% auxiliary agent 1#, 2% auxiliary agent 2#, 0.05% xanthan gum, 0.05% formaldehyde is all by volume；Quality pressed by 5% carbamide
Volume ratio；Water supplies biological pesticide total amount 1L using muscardine and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern)..
2. the biological pesticide of utilization muscardine as claimed in claim 1 and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern)., its feature exists
In described utilization muscardine dilutes 1000 times with the aqueous suspension agent of the biological pesticide of bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern)., in vain
Stiff bacterium spore final concentration of 1 × 104Individual spore/ml, bacillus thuringiensiss final concentration of 1 × 107Individual spore/ml.
3. the preparation of the biological pesticide of a kind of utilization muscardine as claimed in claim 1 and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Method is it is characterised in that described preparation method comprises the following steps：
Measure the high cryptogam of 0.1g muscardine, 10g bacillus thuringiensiss, 6% auxiliary agent 1#, 2% auxiliary agent 2#, 0.05% xanthan respectively
Glue, 0.05% formaldehyde, 5% carbamide, after being fully ground mixing, add sterilized water to supply 1L, sealing preserve.
4. the preparation side of the biological pesticide of utilization muscardine as claimed in claim 3 and bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern).
Method is it is characterised in that the described using method with muscardine and the biological pesticide of bacillus thuringiensiss prevention and control Pyrausta nubilalis (Hubern). includes：
By preparation biological pesticide aqueous suspension agent dilute 1000 times, using high bar spraying machine mid-June Semen Maydiss trumpet period
Carry out field spray preventing and treating, every mu of 20L.
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|---|---|---|---|
| CN201610695297.7A CN106386866A (en) | 2016-08-19 | 2016-08-19 | Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis |
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| CN201610695297.7A CN106386866A (en) | 2016-08-19 | 2016-08-19 | Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN108575456A (en) * | 2018-03-30 | 2018-09-28 | 叶虹 | The biological control method of corn borer |
| CN110679612A (en) * | 2019-10-29 | 2020-01-14 | 宁波市鄞州赛弗兑食品科技有限公司 | Agricultural insecticidal composition for preventing and treating corn borers |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN114145173A (en) * | 2021-11-26 | 2022-03-08 | 蔡三山 | Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine |
| WO2024027266A1 (en) * | 2023-05-12 | 2024-02-08 | 湖北茂盛生物有限公司 | Bacillus thuringiensis biocontrol microbial agent for inhibiting reproduction of pyrausta nubilalis and preparation method therefor |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN108575456A (en) * | 2018-03-30 | 2018-09-28 | 叶虹 | The biological control method of corn borer |
| CN110679612A (en) * | 2019-10-29 | 2020-01-14 | 宁波市鄞州赛弗兑食品科技有限公司 | Agricultural insecticidal composition for preventing and treating corn borers |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN112825865B (en) * | 2020-12-31 | 2022-07-12 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN114145173A (en) * | 2021-11-26 | 2022-03-08 | 蔡三山 | Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine |
| WO2024027266A1 (en) * | 2023-05-12 | 2024-02-08 | 湖北茂盛生物有限公司 | Bacillus thuringiensis biocontrol microbial agent for inhibiting reproduction of pyrausta nubilalis and preparation method therefor |
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