CN114145173A - Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine - Google Patents

Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine Download PDF

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Publication number
CN114145173A
CN114145173A CN202111422344.8A CN202111422344A CN114145173A CN 114145173 A CN114145173 A CN 114145173A CN 202111422344 A CN202111422344 A CN 202111422344A CN 114145173 A CN114145173 A CN 114145173A
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China
Prior art keywords
beauveria bassiana
plant protection
spraying
preventing
fall webworm
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Pending
Application number
CN202111422344.8A
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Chinese (zh)
Inventor
蔡三山
陈京元
张子一
洪承昊
夏健萍
查玉平
徐小文
吴胜兵
陈肆
文坤海
闵水发
陈翔
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Individual
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Individual
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Priority to CN202111422344.8A priority Critical patent/CN114145173A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22Bacillus
    • A01N63/23B. thuringiensis
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27Pseudomonas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom

Abstract

The invention discloses a method for preventing and treating fall webworm larvae by spraying beauveria bassiana on a plant protection machine, wherein the fall webworm larvae are third-generation fall webworm larvae, and the method for preventing and treating the fall webworm larvae comprises the following steps: step 1: adding 300 g of beauveria bassiana high spore powder into the beauveria bassiana dispersible oil suspending agent oil phase, uniformly stirring, and filtering by using gauze to obtain filtrate; step 2: adding 20-30 g of a bacterial preparation into the filtrate to obtain a mixed solution; and step 3: adding clear water into the mixed solution obtained in the step 2; and 4, step 4: and selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying. The invention has the advantages that: 1. according to the invention, the insecticidal microorganism, namely beauveria bassiana, is used as a main component, and the spore is dissolved in water and mixed with a bacterial preparation by using a spore dispersible oil suspending agent technology, so that the problem of environmental unfriendliness of a chemical agent is avoided; 2. adopt plant protection unmanned aerial vehicle to spray efficiently, laborsaving, and apart from the crown distance great, avoid the collision occurence of failure, guarantee the safety of giving medicine to poor free of charge.

Description

Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine
Technical Field
The invention relates to the technical field of prevention and control of hyphantria cunea, in particular to a method for preventing hyphantria cunea larvae by spraying muscardine fungi on a plant protection machine.
Background
At present, an unmanned aerial vehicle is generally used to spray 1.8% of abamectin emulsifiable oil and 1.0% of emamectin benzoate emulsifiable oil respectively under the conditions that the distance from the top end of a tree crown is 2.5-3.5 m and the flying speed is 1.5-3 m/s to prevent and control the fall webworms, although the pesticide application effect is good, the pesticide has great influence on non-targets, and the unmanned aerial vehicle is too close to the tree crown, so that accidents are easy to occur.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the method for preventing and treating the larvae of the fall webworms by spraying the beauveria bassiana on the plant protection machine, which has high efficiency and good effect.
In order to solve the technical problems, the technical scheme provided by the invention is as follows: a method for preventing and treating fall webworm larvae by spraying beauveria bassiana on a plant protection machine, wherein the fall webworm larvae are third-generation fall webworm larvae, the time for preventing and treating the fall webworm larvae is the middle and upper ten days of September, and the method for preventing and treating the fall webworm larvae comprises the following steps:
step 1: adding 300 g of beauveria bassiana high spore powder into 2000-2400 ml of beauveria bassiana dispersible oil suspension agent oil phase while stirring, stirring until the spore powder does not agglomerate, filtering with two layers of gauze, adding 5 l of clear water, and stirring uniformly to obtain filtrate;
step 2: adding 20-30 g of a bacterial preparation into the filtrate, and uniformly stirring and mixing to obtain a mixed solution;
and step 3: adding clear water to the mixed liquid obtained in the step 2 until the volume is 20 liters to obtain liquid medicine, shaking up and injecting the liquid medicine into a medicine chest;
and 4, step 4: selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying,
the beauveria bassiana high spore powder spore content is 1000-2000 hundred million spores/gram, flight parameters of the Xinjiang T20 plant protection unmanned aerial vehicle are set to be flight speed of 1-2 m/second, flight height is 8-10 m away from tree crowns, and spraying amplitude is 8-10 m.
As an improvement, the nozzle of the Xinjiang T20 plant protection unmanned aerial vehicle adopts any one of a fan-shaped nozzle, a 11001 nozzle and a 110015 nozzle.
As an improvement, the dosage of the liquid medicine is 1-2 liters per mu.
Compared with the prior art, the invention has the advantages that: 1. the invention adopts insecticidal microorganism beauveria bassiana as a main component, and dissolves spores in water and bacterial preparation by using a spore dispersible oil suspending agent technology to mix, thereby avoiding the environmental-unfriendly problem of chemical agents; 2. adopt plant protection unmanned aerial vehicle to spray efficiently, laborsaving, and apart from the crown distance great, avoid the collision occurence of failure, guarantee the safety of giving medicine to poor free of charge.
Detailed Description
The present invention is described in further detail below.
Example one
A method for preventing and treating fall webworm larvae by spraying beauveria bassiana on a plant protection machine, wherein the fall webworm larvae are third-generation fall webworm larvae, the time for preventing and treating the fall webworm larvae is in the middle and upper ten days of September, and the method for preventing and treating the fall webworm larvae comprises the following steps:
step 1: adding 2000 ml of beauveria bassiana dispersible oil suspending agent oil phase into 300 g of beauveria bassiana high spore powder while stirring, stirring until the spore powder does not agglomerate, filtering with two layers of gauze, adding 5L of clear water, and stirring to obtain filtrate;
step 2: adding 20 ml of an alcaligenes pseudomonad preparation into the filtrate, and uniformly stirring and mixing to obtain a mixed solution;
and step 3: adding clear water to the mixed liquid obtained in the step 2 until the volume is 20 liters to obtain liquid medicine, shaking up and injecting the liquid medicine into a medicine chest;
and 4, step 4: selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying,
the beauveria bassiana high spore powder spore content is 1000 hundred million spores/g, flight parameters of the Dajiang T20 plant protection unmanned aerial vehicle are set to be 1 m/s of flight speed, the flight height is 8 m away from a tree crown, and the spraying amplitude is 8 m.
The nozzle of the Dajiang T20 plant protection unmanned aerial vehicle adopts a fan-shaped nozzle.
The dosage of the liquid medicine is 1 liter/mu.
Example two
A method for preventing and treating fall webworm larvae by spraying beauveria bassiana on a plant protection machine, wherein the fall webworm larvae are third-generation fall webworm larvae, the time for preventing and treating the fall webworm larvae is in the middle and upper ten days of September, and the method for preventing and treating the fall webworm larvae comprises the following steps:
step 1: adding 300 g of beauveria bassiana high spore powder into 2400 ml of beauveria bassiana dispersible oil suspending agent oil phase while stirring, stirring until the spore powder does not agglomerate, filtering with two layers of gauze, adding 5L of clear water, and stirring uniformly to obtain filtrate;
step 2: adding 30 g of bacillus thuringiensis preparation into the filtrate, and uniformly stirring and mixing to obtain a mixed solution;
and step 3: adding clear water to the mixed liquid obtained in the step 2 until the volume is 20 liters to obtain liquid medicine, shaking up and injecting the liquid medicine into a medicine chest;
and 4, step 4: selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying,
the beauveria bassiana high spore powder spore content is 2000 hundred million spores/gram, flight parameters of the Dajiang T20 plant protection unmanned aerial vehicle are set to be 2 m/s of flight speed, the flight height is 10 m away from a tree crown, and the spraying amplitude is 10 m.
The nozzle of the Dajiang T20 plant protection unmanned aerial vehicle adopts 11001 nozzle.
The dosage of the liquid medicine is 2 liters per mu.
EXAMPLE III
A method for preventing and treating fall webworm larvae by spraying beauveria bassiana on a plant protection machine, wherein the fall webworm larvae are third-generation fall webworm larvae, the time for preventing and treating the fall webworm larvae is in the middle and upper ten days of September, and the method for preventing and treating the fall webworm larvae comprises the following steps:
step 1: adding 2200 ml of beauveria bassiana dispersible oil suspending agent oil phase into 300 g of beauveria bassiana high spore powder while stirring, stirring until the spore powder does not agglomerate, filtering with two layers of gauze, adding 5L of clear water, and stirring to obtain filtrate;
step 2: adding 20 g of bacillus thuringiensis preparation into the filtrate, and uniformly stirring and mixing to obtain a mixed solution;
and step 3: adding clear water to the mixed liquid obtained in the step 2 until the volume is 20 liters to obtain liquid medicine, shaking up and injecting the liquid medicine into a medicine chest;
and 4, step 4: selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying,
the beauveria bassiana high spore powder spore content is 1500 hundred million spores/gram, flight parameters of the Dajiang T20 plant protection unmanned aerial vehicle are set as the flight speed of 1.5 m/s, the flight height is 9 m away from the tree crown, and the spraying amplitude is 9 m.
The nozzle of the Dajiang T20 plant protection unmanned aerial vehicle adopts a 110015 nozzle.
The dosage of the liquid medicine is 1.5 liters per mu.
The following table is a comparison table of the test results of the pesticide effect of the fall webworm larvae in forest:
total number of insects Number of dead insects Number of live insects Mortality rate
3 days after application Process 1 78 20 58 25.6
Treatment 2 97 40 57 41.2
Treatment 3 85 18 67 21.2
7 days after application Process 1 78 38 20 74.4
Treatment 2 97 39 18 81.4
Treatment 3 85 51 16 81.2
14 days after application Process 1 78 18 2 97.4
Treatment 2 97 18 0 100.0
Treatment 3 85 16 0 100.0
Wherein the treatment 1 is beauveria bassiana + BT, the treatment 2 is beauveria bassiana + avermectin, and the treatment 3 is beauveria bassiana + BT + avermectin.
The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (3)

1. A method for preventing and treating fall webworm larva by spraying beauveria bassiana on a plant protection machine is characterized in that: the method for preventing and treating the third generation larvae of the fall webworms comprises the following steps:
step 1: adding 300 g of beauveria bassiana high spore powder into 2000-2400 ml of beauveria bassiana dispersible oil suspending agent oil phase while stirring, stirring until the spore powder does not agglomerate, filtering with two layers of gauze, adding 5L of clear water, and stirring uniformly to obtain filtrate;
step 2: adding 20-30 g of a bacterial preparation into the filtrate, and uniformly stirring and mixing to obtain a mixed solution;
and step 3: adding clear water to the mixed liquid obtained in the step 2 until the volume is 20 liters to obtain liquid medicine, shaking up and injecting the liquid medicine into a medicine chest;
and 4, step 4: selecting a Xinjiang T20 plant protection unmanned aerial vehicle as a carrier for spraying,
the beauveria bassiana high spore powder spore content is 1000-2000 hundred million spores/gram, flight parameters of the Xinjiang T20 plant protection unmanned aerial vehicle are set to be flight speed of 1-2 m/second, flight height is 8-10 m away from tree crowns, and spraying amplitude is 8-10 m.
2. The method for preventing and controlling fall webworm larvae by spraying beauveria bassiana with a plant protection machine according to claim 1, wherein the method comprises the following steps: the nozzle of the Dajiang T20 plant protection unmanned aerial vehicle adopts any one of a fan-shaped nozzle, a 11001 nozzle and a 110015 nozzle.
3. The method for preventing and controlling fall webworm larvae by spraying beauveria bassiana with a plant protection machine according to claim 1, wherein the method comprises the following steps: the dosage of the liquid medicine is 1-2 liters per mu.
CN202111422344.8A 2021-11-26 2021-11-26 Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine Pending CN114145173A (en)

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CN202111422344.8A CN114145173A (en) 2021-11-26 2021-11-26 Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine

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* Cited by examiner, † Cited by third party
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CN102634459A (en) * 2012-02-29 2012-08-15 天津市植物保护研究所 Beauveria bassiana strain having high virulence to fall webworm and application thereof
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CN108402087A (en) * 2017-02-10 2018-08-17 湖北省林业科学研究院 A kind of high cryptogam of muscardine is used for the new method of airplane spray

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