CN1589631A - Compound insecticide of bacillus thuringiensis, muscardine and meliaceae and its preparation method - Google Patents
Compound insecticide of bacillus thuringiensis, muscardine and meliaceae and its preparation method Download PDFInfo
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- CN1589631A CN1589631A CN 03155292 CN03155292A CN1589631A CN 1589631 A CN1589631 A CN 1589631A CN 03155292 CN03155292 CN 03155292 CN 03155292 A CN03155292 A CN 03155292A CN 1589631 A CN1589631 A CN 1589631A
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Abstract
A composite insecticide contains Bacillus thuringienis, muscardine and nimbin, and is prepared from the gemma of Bacillus thuringienis and muscardine, and nim seed powder through proportionally mixing.
Description
Technical field
The present invention relates to the production and the compound method of bacillus thuringiensis, white muscardine fungi and nimbin compounded pesticides, belong to field of biological pesticide.This insecticide can be prevented and treated various pests, can be in a plurality of fields extensive uses such as agricultural, forestry, animal husbandry.
Background technology
Bacillus thuringiensis (bacillus thuringiensis is called for short Bt) is the current maximum of output in the world, a most widely used bacterioid insecticide, accounts for more than 70% of whole biopesticide.China starts from 1958 to the research of bacillus thuringiensis, begins to drop into industrial production the seventies, present domestic tens manufacturers that have, and annual production has reached about 30,000 tons.The Bt insecticide is being made huge contribution aspect prevention and elimination of disease and pests, raising grain and the yield of commercial crops, but because it mainly is by stomach poison function, drug effect is slow, insecticidal effect is not thorough, the report that bacillus thuringiensis (Bt) has been developed immunity to drugs at present in addition relevant for insect, so popularizing of it has been subjected to certain restriction.White muscardine fungi (beauverin bassiaua) is a kind of insect pathogenic fungus of broad spectrum activity, can both be parasitic to more than 700 kinds of harmful insects, pathogenic strong, adaptability is strong, lasting period is long, can prevent and treat lepidoptera pest and coleopteran pest on the various crop, as cabbage caterpillar, diamond-back moth, cotton bollworm, corn borer, chafer and soil insect grub, mole cricket etc., pine caterpillars there is special efficacy, but because the desinsection active principle of white muscardine fungi is the spore of living, therefore it is higher to the dispenser environment requirement, the higher then natural death of temperature was lost efficacy, and therefore used its effect to be difficult to ensure separately.Nimbin (azadirachtin) is a kind of broad spectrum activity vegetable insecticide that extracts in seal chinaberry kind, can prevent and treat 200 various pests, has good insecticidal activity, study, development and utilization by chinaberry to printing for existing nearly 20 countries in the present whole world, insect is difficult for developing immunity to drugs in the use, its shortcoming is unstable, and the performance drug effect is slower.
In order to overcome many defectives that these single type insecticides exist, people can carry out reasonably combination collocation to some for compatible biopesticide usually, make various compounded pesticides, various effective insect killing substances can be strengthened mutually, replenish mutually, thereby reach the desinsection scope of having widened biopesticide, the purpose of accelerating desinsection speed, enhancing killing ability, compare with the insecticide of single type, it has characteristics such as production cost is low, good disinsection effect, using dosage is few, the drug effect phase long, insect is difficult for developing immunity to drugs, and therefore has vast potential for future development.
Summary of the invention
The object of the present invention is to provide a kind of production cost is low, the desinsection scope wide, desinsection speed is fast, integrated control effect is good biopesticide compound insecticidal agent prescription and production and compound method.
Concrete technical scheme of the present invention is, with bacillus thuringiensis (Bt), white muscardine fungi and nimbin is the Main Ingredients and Appearance of biological agriculture chemical compounding insecticide, produce bacillus thuringiensis (Bt) gemma and beauveria bassiana spore composite thallus with the medium that raw materials such as wheat bran, dregs of beans, cavings, corn flour, silk cocoon powder are formed by two step solid state fermentations, pulverize after drying, again with pulverize after neem seed mix by proportioning.
Two step solid state fermentation production technologies of the present invention are:
1. the component and the weight proportion of medium composition medium are: wheat bran 60～65%, dregs of beans 18～22%, cavings 4～6%, corn flour 5～8%, silk cocoon powder 2.5～3%, pulverized limestone 2.5～3.5%, the ratio of water and medium is 1.2～1.3: 1, and it is standard that what of pulverized limestone addition reach 7.2～7.4 with the pH value of medium;
2. put into autoclave after the medium sterilization medium mixes by proportioning and sterilize, 121 ℃ of sterilizations of temperature were sterilized 40～60 minutes in 20 minutes or 100 ℃;
3. bacillus thuringiensis (Bt) inoculation fermentation selects for use bacillus thuringiensis (CCTCC numbers AB93100), bacillus thuringiensis galleria mellonella waxmoth subspecies (CCTCC numbers AB91005), bacillus thuringiensis pine snout moth's larva subspecies (CCTCC numbers AB91006), bacillus thuringiensis Ku Ersi tank subspecies (CCTCC numbers AB90011) to make to produce bacterial classification and carry out cooperative fermentation.The water-bath in 70 ℃ hot water of each bacterial classification together is inoculated into after 7～10 minutes on the medium, again with medium at the thin layer that is tiled on the shallow tray fermentation frame about 2 centimetres, keep 25 ℃～29 ℃ room temperature fermentation 40～48 hours;
4. the white muscardine fungi inoculation fermentation inserts the spore of white muscardine fungi (CCTCC numbers AF93313) during in 70% left and right sides at bacillus thuringiensis (Bt) fermentation 40～48 hours, gemma production rate, then cultivates at normal temperatures 4～7 days again;
5. after the dry fermentation ends, medium is carried out vacuum cooling drying;
6. pulverize medium powder with drying and be broken into powder below 300 orders, packing storage.
Biopesticide compounded pesticides Main Ingredients and Appearance weight proportion of the present invention is: neem seed 20%～35%, bacillus thuringiensis (Bt) and white muscardine fungi solid culture compound 65%～80%.
Complex biological agricultural insecticide agent compounding method of the present invention is: first medium with neem seed and oven dry is ground into the fine powder below 300 orders respectively; join in the mixer; add the synergist (as EGS) account for compounded pesticides gross weight 1～3%, 1～3% bright protective agent (as P81) and 1～3% emulsifier (as sodium lignosulfonate) again, got final product in 40～60 minutes with 150～200 rev/mins rotating speeds mixing.
Complex biological agricultural chemical insecticide of the present invention, every gram contain bacillus thuringiensis (Bt) gemma amount more than 20,000,000,000, and white muscardine fungi lives spore count more than 10,000,000,000, and nimbin content is more than 0.12%, and the toxicity of product has reached requirement.
Complex biological agricultural chemical insecticide of the present invention has the following advantages:
1. raw material sources are wide, and production cost is low, and production technology is simple;
2. the desinsection scope is wide, can kill the various insects of Lepidoptera, coleoptera, Homoptera etc., and various soil insects and parasite are had good control efficiency;
3. desinsection speed is fast, and first day insect population goes down rate more than 60% after the medication, medication after three days control efficiency can reach more than 90%;
4. dosage is few, and the drug effect phase is long, and integrated control effect is good, and insect is difficult to develop immunity to drugs.
Embodiment
In order to understand content of the present invention better, the present invention is further illustrated with embodiment below:
By weight ratio: wheat bran 65%, dregs of beans 20%, cavings 4%, corn flour 5%, silk cocoon powder 3%, pulverized limestone 3%, the ratio of water and medium is to put into autoclave after 1.2: 1 ratio mixes various raw materials, 121 ℃ of temperature sterilization 20 minutes, it is standby to be cooled to 30 ℃ of left and right sides; Bacillus thuringiensis (CCTCC numbers AB93100), bacillus thuringiensis galleria mellonella waxmoth subspecies (CCTCC numbers AB91005), bacillus thuringiensis pine snout moth's larva subspecies (CCTCC numbers AB91006), bacillus thuringiensis Ku Ersi tank subspecies (CCTCC numbers AB90011) water-bath in 70 ℃ hot water is inoculated on the medium after 7～10 minutes together, again with medium at the thin layer that is tiled on the shallow tray fermentation frame about 2 centimetres, keep 25 ℃～29 ℃ room temperature to ferment 40～48 hours, the gemma production rate inserts the spore of white muscardine fungi (CCTCC numbers AF93313) when 70% left and right sides, then cultivated at normal temperatures 4～7 days again.After the fermentation ends, medium is carried out vacuum cooling drying, final powder is broken into the following powder of 300 orders.Get 70 kilograms in this powder, 30 kilograms of the neem seed fine powders below 300 orders, 2 kilograms of 2 kilograms of 2 kilograms of synergist EGS, bright protective agent P81 and sodium lignosulfonates add in the mixer together, mix getting final product in 60 minutes with 200 rev/mins rotating speeds.
Claims (3)
1. a bacillus thuringiensis (Bt), white muscardine fungi and nimbin compounded pesticides, with bacillus thuringiensis (Bt), white muscardine fungi and nimbin is the Main Ingredients and Appearance of biological agriculture chemical compounding insecticide, produce bacillus thuringiensis (Bt) gemma and beauveria bassiana spore composite thallus with the medium that raw materials such as wheat bran, rice bran, corn flour, silk cocoon powder are formed by two step solid state fermentations, pulverize after drying, again with pulverize after neem seed mix by proportioning, it is characterized in that two following step solid state fermentation production technologies:
1.. component and weight proportion that medium is formed medium are: wheat bran 60～65%, dregs of beans 18～22%, cavings 4～6%, corn flour 5～8%, silk cocoon powder 2.5～3%, pulverized limestone 2.5～3.5%, the ratio of water and medium is 1.2～1.3: 1, and it is standard that what of pulverized limestone addition reach 7.2～7.4 with the pH value of medium;
2.. put into autoclave after the medium sterilization medium mixes by proportioning and sterilize, 121 ℃ of sterilizations of temperature were sterilized 40～60 minutes in 20 minutes or 100 ℃;
3.. bacillus thuringiensis (Bt) inoculation fermentation selects for use bacillus thuringiensis (CCTCC numbers AB93100), bacillus thuringiensis galleria mellonella waxmoth subspecies (CCTCC numbers AB91005), bacillus thuringiensis pine snout moth's larva subspecies (CCTCC numbers AB91006), bacillus thuringiensis Ku Ersi tank subspecies (CCTCC numbers AB90011) to make to produce bacterial classification and carry out cooperative fermentation.The water-bath in 70 ℃ hot water of each bacterial classification together is inoculated into after 7～10 minutes on the medium, again with medium at the thin layer that is tiled on the shallow tray fermentation frame about 2 centimetres, keep 25 ℃～29 ℃ room temperature fermentation 40～48 hours;
4.. the white muscardine fungi inoculation fermentation was bacillus thuringiensis (Bt) fermentation 40～48 hours, and the gemma production rate inserts the spore of white muscardine fungi (CCTCC numbers AF93313) when 70% left and right sides, then cultivated at normal temperatures 4～7 days again;
5.. after the dry fermentation ends, medium is carried out vacuum cooling drying;
6.. pulverize medium powder with drying and be broken into powder below 300 orders, packing storage.
2. bacillus thuringiensis according to claim 1 (Bt), white muscardine fungi and nimbin compounded pesticides, it is characterized in that, the Main Ingredients and Appearance weight proportion is: neem seed 20%～35%, bacillus thuringiensis (Bt) and white muscardine fungi solid culture compound 65%～80%.
3. bacillus thuringiensis according to claim 1 (Bt), white muscardine fungi and nimbin compounded pesticides; it is characterized in that; compound method is: the medium with neem seed and oven dry is ground into 300 purpose fine powders respectively earlier; join in the mixer; add the synergist (as EGS) account for compounded pesticides gross weight 1～3%, 1～3% bright protective agent (as P81) and 1～3% emulsifier (as sodium lignosulfonate) again, got final product in 40～60 minutes with 150～200 rev/mins rotating speeds mixing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03155292 CN1589631A (en) | 2003-08-29 | 2003-08-29 | Compound insecticide of bacillus thuringiensis, muscardine and meliaceae and its preparation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 03155292 CN1589631A (en) | 2003-08-29 | 2003-08-29 | Compound insecticide of bacillus thuringiensis, muscardine and meliaceae and its preparation method |
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| CN1589631A true CN1589631A (en) | 2005-03-09 |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482359B (en) * | 2009-02-11 | 2010-04-14 | 中国农业科学院植物保护研究所 | Spore powder drying method |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN105994439A (en) * | 2016-05-25 | 2016-10-12 | 广西南宁市圣农科技开发有限公司 | Nontoxic and residue-free efficient plant protectant and preparation method thereof |
| CN106386866A (en) * | 2016-08-19 | 2017-02-15 | 吉林省农业科学院 | Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis |
| CN106561721A (en) * | 2016-11-09 | 2017-04-19 | 西北农林科技大学 | Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof |
| CN106942277A (en) * | 2017-03-21 | 2017-07-14 | 山东礽健生物科技有限公司 | A kind of biological pesticide and its production method for preventing and treating fragrant-flowered garlic maggot |
| CN107156179A (en) * | 2017-07-11 | 2017-09-15 | 李郑松 | A kind of preparation method of pure natural microbial pesticide |
| CN107333799A (en) * | 2017-05-27 | 2017-11-10 | 兰溪市奥而特农业科技有限公司 | For controlling the medicament of the stupid moth of apple in orchard |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN114145173A (en) * | 2021-11-26 | 2022-03-08 | 蔡三山 | Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine |
| CN115462391A (en) * | 2022-08-25 | 2022-12-13 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Agricultural insecticidal composition and preparation method and application thereof |
-
2003
- 2003-08-29 CN CN 03155292 patent/CN1589631A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101482359B (en) * | 2009-02-11 | 2010-04-14 | 中国农业科学院植物保护研究所 | Spore powder drying method |
| CN102038001A (en) * | 2010-12-03 | 2011-05-04 | 成都绿金高新技术股份有限公司 | Azadirachtin and bacillus subtilis mixed water dispersible granule |
| CN102687731A (en) * | 2012-06-20 | 2012-09-26 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN102687731B (en) * | 2012-06-20 | 2013-11-13 | 东北林业大学 | Wettable powder of bacillus thuringiensis and beauveria bassiana |
| CN105994439A (en) * | 2016-05-25 | 2016-10-12 | 广西南宁市圣农科技开发有限公司 | Nontoxic and residue-free efficient plant protectant and preparation method thereof |
| CN106386866A (en) * | 2016-08-19 | 2017-02-15 | 吉林省农业科学院 | Biopesticide for preventing and controlling corn borers through beauveria bassiana and bacillus thuringiensis |
| CN106561721A (en) * | 2016-11-09 | 2017-04-19 | 西北农林科技大学 | Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof |
| CN106942277A (en) * | 2017-03-21 | 2017-07-14 | 山东礽健生物科技有限公司 | A kind of biological pesticide and its production method for preventing and treating fragrant-flowered garlic maggot |
| CN107333799A (en) * | 2017-05-27 | 2017-11-10 | 兰溪市奥而特农业科技有限公司 | For controlling the medicament of the stupid moth of apple in orchard |
| CN107156179A (en) * | 2017-07-11 | 2017-09-15 | 李郑松 | A kind of preparation method of pure natural microbial pesticide |
| CN112825865A (en) * | 2020-12-31 | 2021-05-25 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN112825865B (en) * | 2020-12-31 | 2022-07-12 | 吉林省农业科学院 | Insecticidal composition, application thereof and insecticide |
| CN114145173A (en) * | 2021-11-26 | 2022-03-08 | 蔡三山 | Method for preventing and treating fall webworm larvae by spraying beauveria bassiana on plant protection machine |
| CN115462391A (en) * | 2022-08-25 | 2022-12-13 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Agricultural insecticidal composition and preparation method and application thereof |
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