CN106561721A - Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof - Google Patents
Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof Download PDFInfo
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- CN106561721A CN106561721A CN201610986105.8A CN201610986105A CN106561721A CN 106561721 A CN106561721 A CN 106561721A CN 201610986105 A CN201610986105 A CN 201610986105A CN 106561721 A CN106561721 A CN 106561721A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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Abstract
The invention provides a ground application type atrijuglans hetaohei yang microbial insecticide formed by compounding a beauveria bassiana strain 1 suspension, a beauveria bassiana strain 2 suspension, a Bt cracked crude protein, streptomycin, ampicillin, a norcantharidin solution, Tween-100 and water. With use of a high-efficiency insecticidal microorganism combined synergist, heliodinidae overwintering larvae can be effectively killed with extremely small dosage, and pesticide residues and environmental pollution are not caused; convenient application comprises that the insecticide is applied to soil under trees; the insecticide is quite suitable for farmers in mountainous areas to prevent and treat atrijuglans hetaohei yang by a convenient and feasible way, and also reduces the cost of prevention and treatment.
Description
Technical field
The invention belongs to microbial insecticide technical field, and in particular to a kind of Types of Medicine Atrijuglans hetaohei microorganism of ground
Insecticide and preparation method and using method.
Background technology
Semen Juglandiss be China's hill farmer increase income, one of the Main Economic forest got rich, particularly in western China, core
Fructus Persicae has become a kind of important forestry pillar industry.In China's Semen Juglandiss producing region, Semen Juglandiss pest and disease damage causes fruit quality and yield
Significantly decline, seriously govern the development of China's Walnut Industry, wherein Atrijuglans hetaohei China's Semen Juglandiss main producing region Shaanxi, four
The provinces and regions such as river, Hebei seriously occur, and cause fruit pre-abscission, Radix osteomelis schwerinais, secondary fruit.Due to the harm of Atrijuglans hetaohei, directly
Significantly the underproduction, fruit quality are decreased obviously to cause Semen Juglandiss, cause Semen Juglandiss Planting household income to be remarkably decreased, and have influence on Semen Juglandiss
Mouth is earned foreign exchange.Dry fruit is eaten or directly eats raw because Semen Juglandiss belong to, the administration of chemical pesticide not only pollutes the environment, also resulted in
High pesticide residues and endanger consumer health.Tall and big plus walnut, in mountain area, administration device and high pressure equipment could be by medicines
Spray application on tree crown, chemical pesticide control lifts limb moth and is difficult to carry out in Semen Juglandiss producing region.
The content of the invention
The purpose of the present invention be overcome traditional chemical pesticide control cannot effective control lift the defect of limb moth, by efficiently micro-
A kind of mode that life assemblage and the lower dispenser of tree are combined, there is provided ground Types of Medicine Atrijuglans hetaohei microbial insecticide.
The technical scheme that the present invention is provided is as follows：
A kind of ground Types of Medicine Atrijuglans hetaohei microbial insecticide, is suspended by the suspension of muscardine bacterial strain 1, muscardine bacterial strain 2
Liquid, Bt cracking crude protein, streptomycin, ampicillin, cantharidin solution, tween -100 and water compounding are formed.
Every liter of ground content 10000-200000 of muscardine bacterial strain 1 in Types of Medicine Atrijuglans hetaohei microbial insecticide,
Content 10000-200000 of muscardine bacterial strain 2, Bt cracking crude protein content 10-1000 g, cantharidin content
0.001-1.0g, content 1-50ml of tween -100.
Every liter of ground content of muscardine bacterial strain 1 and muscardine bacterial strain 2 in Types of Medicine Atrijuglans hetaohei microbial insecticide
It is 200000.
Bt cracking crude protein content is 1000 g/L in every liter of ground Types of Medicine Atrijuglans hetaohei microbial insecticide.
Every liter of ground cantharidin content 1.0g/L in Types of Medicine Atrijuglans hetaohei microbial insecticide.
Every liter of ground Types of Medicine Atrijuglans hetaohei microbial insecticide streptomycin and ampicillin content are respectively
200mg/L。
Present invention also offers a kind of ground Types of Medicine Atrijuglans hetaohei process for preparing microbial insecticide, will match somebody with somebody successively
The muscardine bacterial strain 1 of Fang Hanliang, muscardine bacterial strain 2, Bt cracking crude protein mix homogeneously, then add successively again formulation content
Streptomycin, ampicillin, cantharidin, tween -100, add water to configuration amount, are sufficiently mixed uniform bottling.
Present invention also offers a kind of using method of ground with Types of Medicine Atrijuglans hetaohei microbial insecticide, Semen Juglandiss into
The ripe previous moon is sprayed in the lower crown mapping scope soil surface of tree, and interval sprays once for two weeks, sprays altogether twice.
Shaken up using front firmly concussion, 1000 times of administrations are diluted afterwards.
The invention has the beneficial effects as follows：
1st, pollution-free high-efficient parasite killing：The present invention utilizes efficient insecticide antimicrobial composition synergist, can have with the dosage of very little
Effect kill lift limb moth Overwintering Larvae, because active insecticidal components are microorganisms present in nature, do not cause pesticide residues and
Environmental pollution.
2nd, apply convenient：Using the lower soil pesticide of tree, hill farmer is especially suitable in convenient feasible mode and prevents and treats Semen Juglandiss
Limb moth is lifted, while reducing cost accounting.
To be described in further details below.
Specific embodiment
The invention provides a kind of ground Types of Medicine Atrijuglans hetaohei microbial insecticide, by the suspension of muscardine bacterial strain 1,
The suspension of muscardine bacterial strain 2, Bt cracking crude protein, streptomycin, ampicillin, cantharidin solution, tween -100 and
Water compounding is formed.
Embodiment 1：
A kind of ground Types of Medicine Atrijuglans hetaohei microbial insecticide is present embodiments provided, limb is lifted in every liter of ground with Types of Medicine Semen Juglandiss
Content 10000-200000 of muscardine bacterial strain 1 is individual in moth microbial insecticide, content 10000-200000 of muscardine bacterial strain 2 is individual,
Bt cracks crude protein content 10-1000 g, cantharidin content 0.001-1.0g, content 1-50ml of tween -100.
Embodiment 2：
On the basis of embodiment 1, the Atrijuglans hetaohei microbial insecticide of 1L is prepared：
（1）It is prepared by Bombyx Batryticatus bacterium suspension mother solution：
Muscardine bacterial strain 1（Beauveria bassiana separation strains Qin-118）, it is isolated from Qinling Mountains Guanyin Mountain forest zone, routine side
Method is separated；Bacterial strain is stored in Xibei Univ. of Agricultural ＆ Forest Science ＆ Technology plant protection institute insecticide and utilizes laboratory with microbial resources.
Muscardine bacterial strain 2（B. bassiana separation strains Qin-17）；It is isolated from Qinling Mountains Power Plant EIP forest farm, conventional method point
From；Bacterial strain is stored in Xibei Univ. of Agricultural ＆ Forest Science ＆ Technology plant protection institute insecticide and utilizes laboratory with microbial resources.
Both the above separation strains, are produced respectively by solid fermentation, obtain spore powder.Muscardine bacterial strain is prepared with sterilized water
Suspension mother solution adjustment concentration is 108Individual/L.
（2）It is prepared by Bt cracking crude protein mother solutions：Bt bacterial strains are Bacillus thuringiensis separation strains AKF-1（Contain
Cry2Ab genes）.Strains separation is separated from Ankang Ziyang County forest zone, conventional method；Bacterial strain is stored in northwest agriculture and forestry science and technology
University plant protection institute insecticide utilizes laboratory with microbial resources.
Using LB solution culture fermentation cultures, centrifugal enrichment thalline.With volume ratio 1:5 sterilized water resuspension, ultrasound
After ripple is broken, crack protein liquid is made.Coomassie Brilliant Blue determines cracking crude protein liquid protein content, is then adjusted with sterilized water
Protein concentration to 1.0g/L cracks crude protein mother solution as Bt.
（3）It is prepared by cantharidin mother solution：Take 10g cantharidins to be dissolved in the distilled water that 100mL boils, fill
Divide after dissolving as mother solution, concentration is 100g/L.
（4）Antibiotic mother solution：Ampicillin, streptomycin are 50mg/mL.
Preparation process is as follows：The suspension mother solution of muscardine bacterial strain 1 and each 0.1mL of the suspension mother solution of muscardine bacterial strain 2 are taken, plus
Enter sterilized water 100mL, add the L of Bt cracking crude protein mother solution 10, fully mix.Add the ammonia benzyl of preparation blue or green in mixed liquor
Each 40 L of mycin, streptomycin stock solution, adds norcantharidin mother solution 0.01mL, sterilized water 300mL, adds after mixing and adds
1mL tween -100, then moisturizing is to 1L.It is sufficiently mixed uniform bottling.（Final concentration is respectively：The spore content of muscardine bacterial strain 1
10000/L, 2 spore content of muscardine bacterial strain 10000/L, Bt cracking g/L of crude protein content 10, mg/ of content of streptomycin 2
L, ampicillin content 2mg/L, cantharidin content 1.0mg/L, the % of the content of tween -100 0.1（Volume ratio V/V））.
Embodiment 3：
Bombyx Batryticatus bacterium suspension mother solution, Bt cracking crude protein mother solution in the present embodiment, cantharidin mother solution, ammonia benzyl penicillium sp
Element, the preparation of streptomycin stock solution and concentration are with embodiment 2.
Prepare the Atrijuglans hetaohei microbial insecticide of 1L：
The suspension mother solution of muscardine bacterial strain 1 and each 1.0mL of the suspension mother solution of muscardine bacterial strain 2 are taken, sterilized water 100mL is added,
The L of Bt cracking crude protein mother solution 100 is added, is fully mixed.Ampicillin, streptomycin stock solution each 400 are added in mixed liquor
L, adds norcantharidin mother solution 0.1mL, sterilized water 300mL, adds after mixing and adds 5mL tween -100, then moisturizing is extremely
1L.It is sufficiently mixed uniform bottling.（Final concentration is respectively：100000/the L of spore content of muscardine bacterial strain 1, muscardine bacterium
Strain 2 spore content 100000/L, Bt cracking g/L of crude protein content 100, the mg/L of content of streptomycin 20, ampicillin contain
Amount 20mg/L, cantharidin content 10mg/L, the content of tween -100 0.5%（Volume ratio V/V））.
Embodiment 4：
Bombyx Batryticatus bacterium suspension mother solution, Bt cracking crude protein mother solution in the present embodiment, cantharidin mother solution, ammonia benzyl penicillium sp
Element, the preparation of streptomycin stock solution and concentration are with embodiment 2.
Prepare the Atrijuglans hetaohei microbial insecticide of 1L：
The suspension mother solution of muscardine bacterial strain 1 and each 10mL of the suspension mother solution of muscardine bacterial strain 2 are taken, sterilized water 100mL is added, then is added
Enter Bt cracking crude protein mother solution 1mL, fully mix.Ampicillin, each 2mL of streptomycin stock solution are added in mixed liquor, then is added
Enter norcantharidin mother solution 1.0mL, sterilized water 300mL, add after mixing and add 10mL tween -100, then moisturizing is to 1L.Fill
Divide mix homogeneously bottling.（Final concentration is respectively：The spore content 10 of muscardine bacterial strain 16Individual/L, the spore of muscardine bacterial strain 2 contain
Amount 106It is individual/L, Bt cracking crude protein content 1mg/L, the mg/L of content of streptomycin 100, ampicillin content 100mg/L, nor-
Base Mylabris cellulose content 100mg/L, the content of tween -100 1%（Volume ratio V/V））.
Embodiment 5：
Bombyx Batryticatus bacterium suspension mother solution, Bt cracking crude protein mother solution in the present embodiment, cantharidin mother solution, ammonia benzyl penicillium sp
Element, the preparation of streptomycin stock solution and concentration are with embodiment 2.
Prepare the Atrijuglans hetaohei microbial insecticide of 1L：
The each 100mL of above-mentioned two bacterial strains spore suspension is taken, sterilized water 100mL is added, Bt cracking crude protein mother solution 5mL are added,
Fully mix.Ampicillin, each 4mL of streptomycin stock solution are added in mixed liquor, norcantharidin mother solution 10mL, nothing is added
Bacterium water 300mL, adds after mixing and adds 50mL tween -100, then moisturizing is to 1L.It is sufficiently mixed uniform bottling.（It is dense eventually
Degree is respectively：The spore content 10 of muscardine bacterial strain 17Individual/L, the spore content 10 of muscardine bacterial strain 27Individual/L, Bt cracking crude protein contains
Amount 5mg/L, the mg/L of content of streptomycin 200, ampicillin content 200mg/L, cantharidin content 1.0g/L, tween-
100 contents 5%（Volume ratio V/V））.
Embodiment 6：
A kind of using method of ground Types of Medicine Atrijuglans hetaohei microbial insecticide is present embodiments provided, before Semen Juglandiss maturation
Spray in the lower crown mapping scope soil surface of tree within one month, interval sprays once for two weeks, sprays altogether twice.Exerted oneself using front
Concussion shakes up, and 1000 times of administrations are diluted afterwards.
Insecticidal activity assay
Embodiment 2-5 of Atrijuglans hetaohei microbial insecticide of the present invention with there is Atrijuglans hetaohei walnut as material,
The late summer Semen Juglandiss ripe previous moon, spray in the lower crown mapping scope soil surface of tree, interval sprays once for two weeks, altogether
Spray twice, the Second Year beginning of spring checks Overwintering Larvae quantity in soil, to determine prevention effect.
（1）Atrijuglans hetaohei microbial insecticide working solution of the present invention is prepared：
Reagent agent：Atrijuglans hetaohei microbial insecticide
1000 times are diluted with tap water, by following process, 1 repetition of per group of 10 tree, 3 repetitions of every kind of process：
A) matched group：After the rain under conditions of ground moistening, with hoe is shallow walnut tree disk topsoil is turned over（About 5cm or so）, so
The tap water of equivalent is only sprayed afterwards.
B) group is prevented and treated：After the rain under conditions of ground moistening, with hoe is shallow walnut tree disk topsoil is turned over（About 5cm is left
It is right）, then the Atrijuglans hetaohei microbial insecticide for diluting is sprayed, uniformly spray the lower crown mapping scope soil surface of tree i.e.
Can.（Preventing and treating group is 4 groups, respectively is the Atrijuglans hetaohei microbial insecticide of embodiment 2-5）.
C) after Atrijuglans hetaohei microbial insecticide is applied 2 months, in dispenser tree disk 1m is taken2Area sample prescription is cut the earth, and is cut the earth deep
Degree 40cm, goes through Semen Juglandiss in soil and lifts limb moth larvae quantity and fatality rate.
（2）Atrijuglans hetaohei microbial insecticide prevention effect of the present invention：
After Atrijuglans hetaohei microbial insecticide is applied 2 months, check that Semen Juglandiss lift limb moth larvae quantity and fatality rate in soil, as a result
Such as table 1.As can be seen that the Atrijuglans hetaohei microbial insecticide of embodiment 2-5 all Atrijuglans hetaohei larva is generated it is very strong
Insecticidal effect, field fatality rate more than 85%, up to 94.5%.
The Atrijuglans hetaohei microbial insecticide prevention effect of table 1
The ground Types of Medicine Atrijuglans hetaohei microbial insecticide of the above-mentioned prevention effect explanation present invention, lifts in field control Semen Juglandiss
Limb moth effect is significant and get twice the result with half the effort.Because traditional chemical pesticide control Atrijuglans hetaohei, large-scale spray apparatus is needed
Can be not only laborious bothersome on pesticide application to tree crown, and cause serious pesticide residues and environmental pollution.Particularly on mountain
Area, is limited by landform and traffic, and chemical pesticide control Atrijuglans hetaohei cannot be adopted at all.The ground Types of Medicine core of the present invention
Fructus Persicae lifts limb moth microbial insecticide, and dispenser can be completed without the need for large-scale spraying equipment, while can greatly save labour and subtract
Few environmental pollution, non agricultural chemical residuum.To realizing that green fruit industry, high-quality woods fruit product have very important realistic meaning.
Material of the various embodiments above without detailed narration can be buied directly from market, the method without detailed narration
The common knowledge of the category industry, does not describe one by one here.
Exemplified as above is only the illustration to the present invention, does not constitute the restriction to protection scope of the present invention, all
It is that design same or analogous with the present invention is belonged within protection scope of the present invention.
Claims (9)
1. a kind of ground Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：By the suspension of muscardine bacterial strain 1, in vain
The suspension of stiff bacteria strain 2, Bt cracking crude protein, streptomycin, ampicillin, cantharidin solution, tween -100 and water
Compounding is formed.
2. a kind of ground according to claim 1 Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：Per liter
Content 10000-200000 of muscardine bacterial strain 1, muscardine bacterial strain 2 contain in the Types of Medicine Atrijuglans hetaohei microbial insecticide of ground
Amount 10000-200000, Bt cracking crude protein content 10-1000 g, cantharidin content 0.001-1.0g, tween-
100 contents 1-50ml.
3. a kind of ground according to claim 1 and 2 Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：
The content of muscardine bacterial strain 1 and muscardine bacterial strain 2 is in every liter of ground Types of Medicine Atrijuglans hetaohei microbial insecticide
200000.
4. a kind of ground according to claim 1 and 2 Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：
Bt cracking crude protein content is 1000 g/L in every liter of ground Types of Medicine Atrijuglans hetaohei microbial insecticide.
5. a kind of ground according to claim 1 and 2 Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：
Every liter of ground cantharidin content 1.0g/L in Types of Medicine Atrijuglans hetaohei microbial insecticide.
6. a kind of ground according to claim 1 and 2 Types of Medicine Atrijuglans hetaohei microbial insecticide, it is characterised in that：
Every liter of ground Types of Medicine Atrijuglans hetaohei microbial insecticide streptomycin and ampicillin content are respectively 200mg/L.
7. a kind of ground according to claim 2 Types of Medicine Atrijuglans hetaohei process for preparing microbial insecticide, it is special
Levy and be：Successively by the muscardine bacterial strain 1 of formulation content, muscardine bacterial strain 2, Bt cracking crude protein mix homogeneously, then again according to
Streptomycin, ampicillin, cantharidin, tween -100 of secondary addition formulation content, add water to configuration amount, fully mixed
Close uniform bottling.
8. a kind of ground according to claim 1 and 2 using method of Types of Medicine Atrijuglans hetaohei microbial insecticide, its
It is characterised by：Spray in the ripe previous moon crown mapping scope soil surface under tree of Semen Juglandiss, interval sprays once for two weeks, one
Spray altogether twice.
9. a kind of ground according to claim 8 using method of Types of Medicine Atrijuglans hetaohei microbial insecticide, it is special
Levy and be：Shaken up using front firmly concussion, 1000 times of administrations are diluted afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610986105.8A CN106561721A (en) | 2016-11-09 | 2016-11-09 | Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof |
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| CN201610986105.8A CN106561721A (en) | 2016-11-09 | 2016-11-09 | Ground application type atrijuglans hetaohei yang microbial insecticide and preparation method thereof |
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Application publication date: 20170419 |