CN102067886A - Beauveryia bassiana pesticide suspoemulsion and preparation method thereof - Google Patents
Beauveryia bassiana pesticide suspoemulsion and preparation method thereof Download PDFInfo
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- CN102067886A CN102067886A CN2011100252672A CN201110025267A CN102067886A CN 102067886 A CN102067886 A CN 102067886A CN 2011100252672 A CN2011100252672 A CN 2011100252672A CN 201110025267 A CN201110025267 A CN 201110025267A CN 102067886 A CN102067886 A CN 102067886A
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Abstract
The invention relates to a beauveryia bassiana pesticide suspoemulsion and a preparation method thereof. The invention is characterized in that the beauveryia bassiana pesticide suspoemulsion includes the following materials in part by weight: 20 to 80 parts of beauveryia bassiana spore powder; 10 to 60 parts of emulsified dispersant; 10 to 30 parts of thickening agent; 10 to 30 parts of suspension stabilizer; 10 to 30 parts of spreader; 10 to 60 parts of sugar; and 1 to 10 parts of peptone. The activity of spores is increased, the variety of chemical aids in use is small, moreover, the suspoemulsion can be easily dissolved in water and degraded so that the environmental pollution can be reduced, the adhesion rate of the spores on the body surfaces of inserts is increased, the infection process of the suspoemulsion is quicker than the infection process of raw powder, the requirement of the formulation production process is low, the method is quite simple, and the application in fields is simple. After being diluted by water, the suspoemulsion is in the form of milky, translucent liquid, emulsification is complete, no sediments exist, the stability is high, and the suspoemulsion does not have foreign odor. The dilution factor is high, the packaging and transportation cost is saved, and the transportation of the pesticide is safe. The invention promotes the popularization and commercialization of bassiana and the development of the rural ecological agriculture in China.
Description
Technical field
The present invention relates to a kind of beauveria bassiana insecticide suspension emulsion and preparation method thereof, be used to prevent and treat pest, is a kind of insecticide at corn borer.
Background technology
Corn is the second largest cereal crops of China, produces 3000 hundred million jin per year, can be used to eat, and also can be used as feed and raw material of industry manufacture alcohol and liquor etc.And corn borer is the primary pest in the Maize Production, not only get the food maize leaf, eat in corn stem or the fruit ear, reduce photosynthesis, influence nutrient transport, also produce various secondary diseases, cause the corn underproduction to degrade, cause maize yield to be lost in 7%-15% throughout the year, the serious time underproduction is more than 50%, even total crop failure, causes enormous economic loss for China's grain-production.Chemical pesticide plays significant feature always in the control of corn borer, increases in grain production and safety problem have been guaranteed, but along with a large amount of, continuous chemical pesticide uses, residue of pesticide are more and more serious through the harm of safety to edible safety and environment, and the highly effective pesticide that environment compatibility is good has become the direction of agricultural chemicals development.White muscardine fungi (
Beauveria bassiana) be a kind of filamentous fungi, be present domestic most widely used insect pathogenic fungus.White muscardine fungi has pathogenic strong, and the desinsection scope is wide, advantages of environment protection, but in control, still have shortcomings such as formulation is single, efficiency is low, desinsection speed is slow, insecticide efficiency is low.At present, white muscardine fungi is the control of corn borer and pine caterpillars the most successful popularization model of China, promotes but be government, and using maximum is the white muscardine fungi pulvis, piles up neatly and air-dusting to seal corn spring; Beauveria bassiana granular formulation also has use, but efficiency is low, fades away.Causing white muscardine fungi is reasons such as the degeneration of bacterial classification virulence, product spore power instability and formulation are single in the main cause of China's popularization and commercialization difficulty, and wherein formulation is single is subject matter.All the time, white muscardine fungi novel form exploitation is exactly a difficult point, main because: one, white muscardine fungi is a living microorganism performance drug effect, is easy to cause the bacterial classification inactivation in the process of development formulation, causes the novel form drug effect of developing low, does not have promotional value; Two, the white muscardine fungi novel form of developing is always based on pulvis, and it is low to use efficiency, is badly in need of developing the drug effect height, being beneficial to the novel form of using; Even three develop effective formulation, formulation preservation and field using method difficulty have greatly also limited to be promoted the use of, so white muscardine fungi is promoted and the commercialization difficulty.This research is developed the pesticides new formulation of white muscardine fungi high efficiency insecticide-applying way under the prerequisite that guarantees white muscardine fungi spore production rate, spore germination rate and virulence.Though the record of some beauveria bassiana formulations is also arranged before this, mainly contains several big classes of wetting powder, granule, finish, suspending agent and oil-suspending agent.The present invention is in use convenient than wetting powder and granule, and safety.Comparing with suspending agent and will be higher than suspending agent, mainly is that emulsifying effectiveness is better than suspending agent.And have some compositions that beauveria bassiana spore is had injury effect in the employed chemical assistant of finish and oil suspending agent.
Summary of the invention
The object of the invention provides a kind of beauveria bassiana insecticide suspension emulsion and preparation method thereof, improves the spore activity, has added certain nutrient component in the prescription, promotes it to produce spore.The medicament use of can directly spraying after water dilution is applicable to farm work very much.
Technical scheme of the present invention is achieved in that a kind of beauveria bassiana insecticide suspension emulsion, it is characterized in that being made up of following raw material by weight: the beauveria bassiana conidial powder: 20-80 part; Emulsifying dispersant: 10-60 part; Thickener: 10-30 part; Suspension stabilizer: 10-30 part; Spreader-sticker: 10-30 part; Sugar: 10-60 part; Peptone: 1-10 part, the white muscardine fungi spore content of beauveria bassiana conidial powder are 50,000,000,000/gram, and the spore rate of living is greater than 90%.
Described emulsifying dispersant is alkylphenol polyoxyethylene formaldehyde condensation products sulphate, phenethyl phenol polyethenoxy ether.Described thickener is an xanthans.
Described suspension stabilizer is a sodium carboxymethylcellulose.
Described spreader-sticker is a polyvinyl alcohol.
Described sugar is glucose.
The preparation method of described suspension emulsion, its step is as follows: at first emulsifying dispersant, thickener, suspension stabilizer, spreader-sticker, sugar, peptone are mixed by weight; Auxiliary agent after mixing is placed on the JQJ-110 type, and power is 7.5KW, joins on the homogenizer of slotted hole stator head, and normal pressure stirs 30min with 4000 revolutions per seconds of speed under closed state, and it is stirred; After auxiliary agent stirs, join and add the beauveria bassiana conidial powder in the skin grinder again; Auxiliary agent and beauveria bassiana conidial powder are to stir 10-20min with 2000 revolutions per seconds of rotating speeds under the normal pressure in the 5-10 μ m skin grinder evenly to get final product in model SYH3-100,3 liters of capacity, fineness.
Beauveria bassiana insecticide suspension emulsion of the present invention can use separately, also can unite use with some chemical insecticides, is applicable to the control agriculture and forestry injurious insect; Be suitable for any insect being had the beauveria bassiana conidial powder of lethal effect, direct dilute with water during use, extension rate 500-5000 doubly more than, the use of can directly spraying after the dilution.
Good effect of the present invention is: with respect to domestic existing other beauveria bassiana formulation, improve the spore activity, few and soluble in water, the easy degraded of using of chemical assistant kind, reduced environmental pollution, spore improves at the adhesive rate of insect body surface, infection processs is faster than former powder speed, the formulation production process is less demanding, method is very easy, and the field is used simple.The translucent liquid that is creamy white behind the dilute with water, emulsification fully, no precipitation, good stability, free from extraneous odour; High packing and cost of transportation, the agricultural chemicals transportation safety saved of extension rate; Promote white muscardine fungi and promoted and commercialization, promoted China's rural ecological agricultural development.
Embodiment
The present invention will be further described below in conjunction with embodiment: prescription all calculates by weight in the scheme, and used chemical assistant all has sale on the market.
Embodiment 1
Getting 20 parts of spore contents is that 50,000,000,000 spore/grams, spore rate alive are greater than the sub-powder of 90% Pure Spores of Beauveria bassiana; 10 parts of alkylphenol polyoxyethylene formaldehyde condensation products sulphate; 10 parts of sodium carboxymethylcelluloses; 10 parts of xanthans; 10 parts of glucose; 1 part of peptone; Surplus is a water.
Be placed on the homogenizer after alkylphenol polyoxyethylene formaldehyde condensation products sulphate, sodium carboxymethylcellulose, xanthans, glucose, peptone mixed by above-mentioned parts by weight and stir, stir 10-15 minute evenly after, the auxiliary agent of mixing is joined in the skin grinder, add the beauveria bassiana conidial powder again.Stir and be beauveria bassiana suspension emulsion 1 ﹟ after evenly in 10 minutes.
Embodiment 2
With embodiment 1 method, getting 40 parts of spore contents is that 50,000,000,000 spore/grams, spore rate alive are greater than the sub-powder of 90% Pure Spores of Beauveria bassiana; 30 parts of phenethyl phenol polyethenoxy ethers; 20 parts of polyvinyl alcohol; 20 parts of xanthans.Be beauveria bassiana suspension emulsion 2 ﹟.
Major parameter is as follows:
Embodiment 3
Beauvericin insecticide suspension emulsion of the present invention compares at control of maize snout moth's larva and the former powder indoor virulence of white muscardine fungi effect
Embodiment 4
Beauvericin insecticide suspension emulsion of the present invention compares at control of maize snout moth's larva and beauveria bassiana granular formulation and chemical pesticide field effect
?
Embodiment 5
Getting 80 parts of spore contents is that 50,000,000,000 spore/grams, spore rate alive are greater than the sub-powder of 90% Pure Spores of Beauveria bassiana; 60 parts of alkylphenol polyoxyethylene formaldehyde condensation products sulphate; 30 parts of sodium carboxymethylcelluloses; 30 parts of xanthans; 60 parts of glucose; 10 parts of peptones; Surplus is a water.
Be placed on the homogenizer after alkylphenol polyoxyethylene formaldehyde condensation products sulphate, sodium carboxymethylcellulose, xanthans, glucose, peptone mixed by above-mentioned parts by weight and stir, stir 10-15 minute evenly after, the auxiliary agent of mixing is joined in the skin grinder, add the beauveria bassiana conidial powder again.Stir and be the beauveria bassiana suspension emulsion after evenly in 10 minutes.
Claims (7)
1. a novel beauveria bassiana suspension emulsion is characterized in that being grouped into by following one-tenth by weight: the beauveria bassiana conidial powder: 20-80 part; Emulsifying dispersant: 10-60 part; Thickener: 10-30 part; Suspension stabilizer: 10-30 part; Spreader-sticker: 10-30 part; Sugar: 10-60 part; Peptone: 1-10 part; The white muscardine fungi spore content of described beauveria bassiana conidial powder is 50,000,000,000/gram, and the spore rate of living is greater than 90%.
2. a kind of novel beauveria bassiana suspension emulsion according to claim 1 is characterized in that described emulsifying dispersant is alkylphenol polyoxyethylene formaldehyde condensation products sulphate, phenethyl phenol polyethenoxy ether.
3. a kind of novel beauveria bassiana suspension emulsion according to claim 1 is characterized in that described thickener is an xanthans.
4. a kind of novel beauveria bassiana suspension emulsion according to claim 1 is characterized in that described suspension stabilizer is a sodium carboxymethylcellulose.
5. a kind of novel beauveria bassiana suspension emulsion according to claim 1 is characterized in that described spreader-sticker is a polyvinyl alcohol.
6. a kind of novel beauveria bassiana suspension emulsion according to claim 1 is characterized in that described sugar is glucose.
7. a kind of novel beauveria bassiana suspension emulsion according to claim 1, the preparation method who it is characterized in that described suspension emulsion, its step is as follows: at first emulsifying dispersant, thickener, suspension stabilizer, spreader-sticker, sugar, peptone are mixed by weight; Auxiliary agent after mixing is placed on the JQJ-110 type, and power is 7.5KW, joins on the homogenizer of slotted hole stator head, and normal pressure stirs 30min with 4000 revolutions per seconds of speed under closed state, and it is stirred; After auxiliary agent stirs, join and add the beauveria bassiana conidial powder in the skin grinder again; Auxiliary agent and beauveria bassiana conidial powder are to stir 10-20min with 2000 revolutions per seconds of rotating speeds under the normal pressure in the 5-10 μ m skin grinder evenly to get final product in model SYH3-100,3 liters of capacity, fineness.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103045488A (en) * | 2012-12-04 | 2013-04-17 | 中国科学院海洋研究所 | Beauveria felina AS-70 strain and application thereof |
| CN103461387A (en) * | 2013-08-31 | 2013-12-25 | 红河蔗保科技有限责任公司 | Beauveria bassiana chlorpyrifos insecticide and preparation method thereof |
| CN104430212A (en) * | 2014-12-17 | 2015-03-25 | 广东省农业科学院蚕业与农产品加工研究所 | Spore suspension for improving yield of white muscardine silkworms and application of spore suspension |
| CN104738097A (en) * | 2015-03-07 | 2015-07-01 | 吉林省农业科学院 | Beauveria bassiana barrel mixed agent and preparation method thereof |
| CN104920477A (en) * | 2015-06-15 | 2015-09-23 | 吉林省农业科学院 | Beauveria bassiana seed coating and preparation method thereof |
| CN105875653A (en) * | 2016-04-15 | 2016-08-24 | 佛山市聚成生化技术研发有限公司 | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide |
| CN106135294A (en) * | 2016-07-22 | 2016-11-23 | 吉林省农业科学院 | A kind of Novel ball beauveria bassiana granule |
| CN108378062A (en) * | 2018-05-08 | 2018-08-10 | 江西正邦生物化工有限责任公司 | A kind of composition pesticide and its application containing beauveria bassiana and metaflumizone |
| CN109329309A (en) * | 2018-11-27 | 2019-02-15 | 何美英 | A kind of biological pesticide and preparation method thereof of the glyceride of waste oil containing residue of soya |
| CN109402161A (en) * | 2018-11-22 | 2019-03-01 | 吉林省农业科学院 | GFP-labeled beauveria bassiana BbofDH1-5-GFP and application thereof |
| CN114514928A (en) * | 2022-02-22 | 2022-05-20 | 青岛农业大学 | Biological pesticide dosage form processing technology and production equipment thereof |
| CN115517250A (en) * | 2022-09-28 | 2022-12-27 | 吉林省农业科学院 | Soil pesticide synergist and preparation method and application thereof |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045488B (en) * | 2012-12-04 | 2014-10-22 | 中国科学院海洋研究所 | Beauveria felina AS-70 strain and application thereof |
| CN103045488A (en) * | 2012-12-04 | 2013-04-17 | 中国科学院海洋研究所 | Beauveria felina AS-70 strain and application thereof |
| CN103461387A (en) * | 2013-08-31 | 2013-12-25 | 红河蔗保科技有限责任公司 | Beauveria bassiana chlorpyrifos insecticide and preparation method thereof |
| CN103461387B (en) * | 2013-08-31 | 2015-05-20 | 红河蔗保科技有限责任公司 | Beauveria bassiana chlorpyrifos insecticide and preparation method thereof |
| CN104430212A (en) * | 2014-12-17 | 2015-03-25 | 广东省农业科学院蚕业与农产品加工研究所 | Spore suspension for improving yield of white muscardine silkworms and application of spore suspension |
| CN104738097A (en) * | 2015-03-07 | 2015-07-01 | 吉林省农业科学院 | Beauveria bassiana barrel mixed agent and preparation method thereof |
| CN104920477A (en) * | 2015-06-15 | 2015-09-23 | 吉林省农业科学院 | Beauveria bassiana seed coating and preparation method thereof |
| CN104920477B (en) * | 2015-06-15 | 2017-12-08 | 吉林省农业科学院 | A kind of beauveria bassiana seed coat agent and preparation method thereof |
| CN105875653B (en) * | 2016-04-15 | 2018-08-17 | 佛山市聚成生化技术研发有限公司 | A kind of beauveria bassiana microbial pesticide and preparation method thereof |
| CN105875653A (en) * | 2016-04-15 | 2016-08-24 | 佛山市聚成生化技术研发有限公司 | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide |
| CN106135294A (en) * | 2016-07-22 | 2016-11-23 | 吉林省农业科学院 | A kind of Novel ball beauveria bassiana granule |
| CN108378062A (en) * | 2018-05-08 | 2018-08-10 | 江西正邦生物化工有限责任公司 | A kind of composition pesticide and its application containing beauveria bassiana and metaflumizone |
| CN109402161A (en) * | 2018-11-22 | 2019-03-01 | 吉林省农业科学院 | GFP-labeled beauveria bassiana BbofDH1-5-GFP and application thereof |
| CN109329309A (en) * | 2018-11-27 | 2019-02-15 | 何美英 | A kind of biological pesticide and preparation method thereof of the glyceride of waste oil containing residue of soya |
| CN114514928A (en) * | 2022-02-22 | 2022-05-20 | 青岛农业大学 | Biological pesticide dosage form processing technology and production equipment thereof |
| CN115517250A (en) * | 2022-09-28 | 2022-12-27 | 吉林省农业科学院 | Soil pesticide synergist and preparation method and application thereof |
| CN115517250B (en) * | 2022-09-28 | 2023-09-19 | 吉林省农业科学院 | Pesticide synergist for soil and preparation method and application thereof |
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Application publication date: 20110525 |