CN102172250A - Preparation method and application of eauveria-spinosad suspending agent - Google Patents
Preparation method and application of eauveria-spinosad suspending agent Download PDFInfo
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- CN102172250A CN102172250A CN2011100712892A CN201110071289A CN102172250A CN 102172250 A CN102172250 A CN 102172250A CN 2011100712892 A CN2011100712892 A CN 2011100712892A CN 201110071289 A CN201110071289 A CN 201110071289A CN 102172250 A CN102172250 A CN 102172250A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention relates to a preparation method and application of q beauveria-spinosad suspending agent. Beauveryia bassiana and spinosad are the active ingredients of the suspending agent which is used for preventing and treating agriculture and forestry injurious insects, such as leptinotarsa decemlineata and acridid and the like. The invention is characterized in that (1) the suspending agent comprises active ingredients, namely the beauveryia bassiana and the spinosad; and (2) the mass ratio of the beauveryia bassiana and the spinosad is (30:1)-(30:2), and the mass percent of the effective components in the preparation is 3.1-10%. The preparation method has the advantages that the product can be dissolved with oil or water; the effective duration of the beauveria vuillemin and the relative quick action of spinosad have complementary advantages, and injurious insects of various crops are treated; and the suspending agent prepared by the method has the advantages of good synergism and permeability, strong adhesivity and evaporation resistance and the like.
Description
Technical field
The present invention relates to a kind of microbial oil suspending agent, is the oil-suspending agent of active ingredient with beauveria bassiana and multiple killing teichomycin specifically, is mainly used in the control of insect pests such as colorado potato beetle and locust.
Background technology
White muscardine fungi (Beauveria) is a kind of modal insect pathogenic fungus, can infect 700 various insects and mite class, and breeding in a large number in the host polypide makes body fluid find that function changes, metabolic disturbance so that dead.The widely dispersed of white muscardine fungi was all found the existence of white muscardine fungi from the high mountain of several meters to more than 2000 meters of height above sea level, and the pathogenicity of white muscardine fungi is strong, to people and animals' crop safety, was one of maximum, most widely used insect pathogenic fungus of research both at home and abroad.
Just because of the host range of white muscardine fungi is wide, popular strong, and to characteristics such as person poultry safeties, for a long time be not studied the person as the important means of pest control, but white muscardine fungi in use exist selectivity strong, infect that speed is slow, control efficiency is subjected to limiting factors such as environmental influence is bigger.
At present, the domestic beauveria bassiana conidium preparation of having succeeded in developing comprises formulations such as pulvis, wetting powder, granule and oil-suspending agent.Registered 14 kinds of formulation products abroad in succession, but in actual applications, beauveria bassiana conidium preparation is still continued to use solid pharmaceutical preparations such as pulvis mostly, its active constituent content is lower, and volume is bigger, brings very big difficulty for transportation, storage and use; And because the hydrophobicity of conidia of beauveria bassiana makes that in use bacterium powder consumption is big, adhesive force is bad, and control efficiency is very unstable.
Multiple killing teichomycin (Spinosad) is the insecticide of a kind of low toxicity, efficient, wide spectrum, low-residual, insect is had tag fast and stomach poison function, and blade is had stronger osmosis, can the effectively preventing Lepidoptera, diptera and thrips insect.Be suitable for using on vegetables, fruit tree, gardens, the crops.
Oil-suspending agent is the high dispersive that forms in non-water system dispersate of one or more pesticide active ingredients, stable suspension system.With formulation ratios such as aqueous suspension agent, wetting powder, water dispersible granules, advantages such as good penetrability, adhesiveness are strong, anti-evaporating, storage endurance that it has.
Summary of the invention
The object of the present invention is to provide a kind of white muscardine fungi-multiple killing teichomycin oil-suspending agent.This oil-suspending agent has been integrated existing fungal oil suspending agent (as obtaining the interim registration number of national Ministry of Agriculture agricultural chemicals: with the advantage separately of aqueous emulsion, be a kind of cost white muscardine fungi low, easy to use-multiple killing teichomycin oil-suspending agent of using the beauveria bassiana goods of LS20091079).
The object of the present invention is achieved like this: the composition proportioning of a kind of white muscardine fungi-multiple killing teichomycin oil-suspending agent accounts for 1%～50% for the high cryptogam of white muscardine fungi by percentage to the quality, multiple killing teichomycin accounts for 0.1%～1%, surfactant comprise 0.5%～8%, suspended oil accounts for 40%～70%.
The mass percent of each component is:
The high cryptogam of above-mentioned white muscardine fungi is the high cryptogam of beauveria bassiana, and spore content is greater than 1 * 10
11Individual/g, the spore rate of living is greater than 85%; Above-mentioned surfactant is a polyglycereol castor-oil plant alcohol ester; Above-mentioned solvent naphtha is the polymerization linseed oil, and effective content is greater than 85%.
1. the preparation of the high cryptogam of white muscardine fungi
Get beauveria bassiana bacterial classification (ACCC32445, obtain by Chinese agriculture microorganism fungus kind preservation administrative center) access sterilization product spore medium (YPDCA commonly used in gnotobasis, YPDCA is the abbreviation of main component, Y: dusty yeast, P: peptone, D: glucose, C: corn soaks powder A: agar) solid plate carries out activation processing; Scraping the good bacterial classification of getting activation makes suspension and adds liquid and produce spore medium (YPDC) and enlarge spawn culture; After 48 hours, its liquid nutrient medium that is connected to after sterilizing in the fermentation tank is carried out liquid fermentation; After cultivating 24h, be connected in the solid culture medium after waiting to expect to sterilize, after mixing, enter to cultivate the room and carry out solid fermentation, the good laggard drying shed of solid culture produce spore is dried processing, treat the solid culture finish-drying after, on airtight extracting machine, carry out the extraction of high cryptogam, finally obtain the high cryptogam of white muscardine fungi, accompanying drawing 1 is seen in technological process.
2. the preparation of white muscardine fungi-multiple killing teichomycin oil-suspending agent
Press formula rate, add high cryptogam of white muscardine fungi and oil emulsion in mixing kettle, carry out high-shear emulsifying with high-shear emulsion machine and stir (8000r/min), 10～15min fully mixes material; With mixed product, add thickener and surfactant and in mixing kettle, proceed high-shear emulsifying stirring (8000r/min), 10～15min, the product that obtains adds the former medicine of multiple killing teichomycin again, and (Tao Shi agricultural science and technology company produces, purity＞90%) carries out last high-shear emulsifying and stir (8000r/min), 15～20min obtains white muscardine fungi-multiple killing teichomycin oil-suspending agent, and accompanying drawing 2 is seen in technological process.
3. the application of white muscardine fungi-multiple killing teichomycin oil-suspending agent
White muscardine fungi of the present invention-how extremely oil-suspending agent is the oil-suspending agent formulation, because of adding the good surfactant of compatibility, add the state that water can form oil-in-water, oil parcel spore during use, sufficient moisture can promote the sprouting of spore, the spore of oil parcel contacts with host's epidermis after the water evaporates, has the characteristic of oil-suspending agent.So the beauveria bassiana spore oil-suspending agent has oil-suspending agent little characteristics affected by environment.
Above-mentioned beauveria bassiana spore oil-suspending agent is easy to use, and dilute with water during use also can use oil can adopt multiple insecticide-applying way as required; Specific practice is: adopt the spraying of ultra low volume technology after diluting 1～10 times with oil or water; Or adopt the low dosage spraying behind 10～100 times of the dilute with waters; Or adopt conventional spray behind 500～1000 times of the dilute with waters.The dilute with water multiple is preferably 3～7 times when adopting the spraying of ultra low volume technology, and the dilute with water multiple is preferably 10～30 times when adopting the spraying of low capacity technology, and the dilute with water multiple is preferably 500～800 times during the routine techniques spraying.
The present invention has the following advantages:
(1) product of the present invention is owing to after adopting multiple killing teichomycin and these two kinds of single agent of white muscardine fungi composite, overcome single with the slow shortcoming of white muscardine fungi drug effect, combine the strong point that original each single agent is had, its toxicity synergy to insects such as colorado potato beetle, locusts is obvious, can significantly improve insecticidal effect, significantly shorten onset time.
(2) product of the present invention is in composite component, and the multiple killing teichomycin usage ratio is single few with ratio, has not only reduced the toxicity and the residual rate of compound formulation, is of value to and preserves the ecological environment, and reduced its production cost, has vast market prospect.
(3) white muscardine fungi of the present invention-multiple killing teichomycin oil-suspending agent preventive effect is stable, and is affected by environment little.Owing to contain surfactant in the preparation, form aqueous emulsion behind the thin up of field, and sporocyst is wrapped in the oil droplet, has oil-suspending agent little characteristics affected by environment, a large amount of moisture can promote the sprouting of spore simultaneously, thereby reduces the dependence of preparation to ambient humidity.
(4) surfactant of product of the present invention is a nonionic, and is good with the fungal spore compatibility in oil-suspending agent, and not obviously influence storage period to oil-suspending agent has kept oil-suspending agent long characteristics storage period.
Beneficial effect of the present invention is
The present invention relates to white muscardine fungi-multiple killing teichomycin oil-suspending agent preparation method and application.The present invention is to be the oil-suspending agent of active ingredient with beauveria bassiana and multiple killing teichomycin, is mainly used in the control of agriculture and forestry injurious insects such as colorado potato beetle and locust.(1) contain active ingredient biopesticide beauveria bassiana and multiple killing teichomycin, all the other are to allow in the agricultural chemicals to use and the acceptable auxiliary element; (2) mass ratio of beauveria bassiana and multiple killing teichomycin was at 30: 1～30: 2, and the mass percentage content of active ingredient in preparation is 3.1%～10%.Product involved in the present invention can be molten with oil or water, and the relative quick-acting of lasting effect of white muscardine fungi and multiple killing teichomycin has obtained mutual supplement with each other's advantages, and a kind of medicament can be prevented and treated the various crop insect; Have advantages such as synergy, good penetrability, adhesiveness are strong, anti-evaporating.
Description of drawings
The process chart of the high cryptogam preparation of Fig. 1 white muscardine fungi.
The process chart of Fig. 2 white muscardine fungi-multiple killing teichomycin oil-suspending agent preparation.
Embodiment
In order to understand invention essence better, describe the technology contents of invention below in detail with example, but the purposes of preventing and treating of the present invention is not limited thereto.Percentage in the following example is the percentage in the preparation.
With 3.2% white muscardine fungi-multiple killing teichomycin oil-suspending agent is example, uses the example of variable concentrations
By the prescription requirement, in mixing kettle, add high cryptogam of white muscardine fungi and oil emulsion, (8000r/min) stirs 10～15min in high shear breast machine (the wide machine-building of the Shanghai prestige high shearing mixing emulsor 100LX of Co., Ltd), and material is fully mixed; With mixed product, add thickener and surfactant in mixing kettle, in high-shear emulsion machine, stir (8000r/min) 10～15min, the product that obtains adds the former medicine of multiple killing teichomycin again, carry out last high-shear emulsifying and stir (8000r/min) 15～20min, must make oil-suspending agent,, measure packing and get product through after the assay was approved.
Surfactant can substitute polyglycereol castor-oil plant alcohol ester with B-101 (ring easily reaches development in agricultural science and technology Co., Ltd in Beijing); Thickener can substitute the octenyl Starch Sodium with sodium carboxymethylcellulose; Solvent naphtha can substitute the polymerization linseed oil with rapeseed oil or soybean oil.
Use these mixture 500 gram/mus to convert water even spraying control colorado potato beetle, 5 days control efficiency reach control efficiency arrival 94.2% in 92%, the 15 day behind the medicine.
Use these mixture 250 gram/mus to convert water even spraying control colorado potato beetle, 5 days control efficiency reach control efficiency arrival 90% in 87.3%, the 15 day behind the medicine.
Do blank with clear water, preventive effect is 0.
Show that from experimental result more white muscardine fungi kills and kills the larva of a large amount of colorado potato beetles fast after the compound oil suspending agent is executed bacterium, and can form the bombys batryticatus rate of radix 20%, execute the bury infection rate of vest of month " Invest, Then Investigate " of bacterium, the bombys batryticatus rate reaches 19% in the soil.Killing property of speed and lasting prevention and control effect are preferably arranged.
Claims (5)
1. white muscardine fungi-multiple killing teichomycin oil-suspending agent preparation method is characterized in that:
(1) contain active ingredient biopesticide beauveria bassiana and multiple killing teichomycin, all the other are to allow in the agricultural chemicals to use and acceptable auxiliary element surfactant, thickener and solvent naphtha;
(2) mass ratio of beauveria bassiana and multiple killing teichomycin was at 30: 1～30: 2, and the mass percentage content of active ingredient in preparation is 2%～10%.
2. a kind of white muscardine fungi according to claim 1-multiple killing teichomycin oil-suspending agent is characterized in that the mass percent of each component is:
3. according to claim 1 and 2 described a kind of white muscardine fungi-multiple killing teichomycin oil-suspending agents, it is characterized in that its preferred ingredient and mass percent thereof are:
4. according to the described a kind of white muscardine fungi of claim 1-3-multiple killing teichomycin oil-suspending agent, it is characterized in that surfactant can substitute polyglycereol castor-oil plant alcohol ester with B-101; Thickener can substitute the octenyl Starch Sodium with sodium carboxymethylcellulose; Solvent naphtha can substitute the polymerization linseed oil with rapeseed oil or soybean oil.
5. the application of white muscardine fungi-multiple killing teichomycin oil-suspending agent is characterized in that this oil-suspending agent is mainly used in various pests such as control colorado potato beetle, locust.
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| CN2011100712892A CN102172250A (en) | 2011-03-24 | 2011-03-24 | Preparation method and application of eauveria-spinosad suspending agent |
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Cited By (8)
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|---|---|---|---|---|
| CN103553842A (en) * | 2013-11-19 | 2014-02-05 | 广西新方向化学工业有限公司 | Fungus insecticide fertilizer |
| CN103875716A (en) * | 2014-03-07 | 2014-06-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Beauveria bassiana.bacillus thuringiensis.spinosyns composition and use of beauveria bassiana.bacillus thuringiensis.spinosyns composition for preventing and controlling rice stem borers |
| CN106719846A (en) * | 2016-11-22 | 2017-05-31 | 恒大园林集团有限公司 | A kind of complex ecological agricultural chemicals and preparation method thereof |
| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN108633916A (en) * | 2018-07-03 | 2018-10-12 | 湖南泽丰农化有限公司 | A kind of Pesticidal combination containing beauveria bassiana and ethyl pleocidin |
| CN110184197A (en) * | 2019-05-15 | 2019-08-30 | 云南星耀生物制品有限公司 | A kind of beauveria bassiana oil-suspending agent |
| CN113142245A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103553842A (en) * | 2013-11-19 | 2014-02-05 | 广西新方向化学工业有限公司 | Fungus insecticide fertilizer |
| CN103875716A (en) * | 2014-03-07 | 2014-06-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Beauveria bassiana.bacillus thuringiensis.spinosyns composition and use of beauveria bassiana.bacillus thuringiensis.spinosyns composition for preventing and controlling rice stem borers |
| CN106719846A (en) * | 2016-11-22 | 2017-05-31 | 恒大园林集团有限公司 | A kind of complex ecological agricultural chemicals and preparation method thereof |
| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN108633916A (en) * | 2018-07-03 | 2018-10-12 | 湖南泽丰农化有限公司 | A kind of Pesticidal combination containing beauveria bassiana and ethyl pleocidin |
| CN110184197A (en) * | 2019-05-15 | 2019-08-30 | 云南星耀生物制品有限公司 | A kind of beauveria bassiana oil-suspending agent |
| CN110184197B (en) * | 2019-05-15 | 2020-04-03 | 云南星耀生物制品有限公司 | Beauveria bassiana oil suspending agent |
| CN113142245A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
| CN113142244A (en) * | 2021-01-05 | 2021-07-23 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142244B (en) * | 2021-01-05 | 2022-03-29 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram |
| CN113142245B (en) * | 2021-01-05 | 2022-04-05 | 华南农业大学 | Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram |
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Application publication date: 20110907 |