CN103875716A - Beauveria bassiana.bacillus thuringiensis.spinosyns composition and use of beauveria bassiana.bacillus thuringiensis.spinosyns composition for preventing and controlling rice stem borers - Google Patents

Beauveria bassiana.bacillus thuringiensis.spinosyns composition and use of beauveria bassiana.bacillus thuringiensis.spinosyns composition for preventing and controlling rice stem borers Download PDF

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CN103875716A
CN103875716A CN201410084227.9A CN201410084227A CN103875716A CN 103875716 A CN103875716 A CN 103875716A CN 201410084227 A CN201410084227 A CN 201410084227A CN 103875716 A CN103875716 A CN 103875716A
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bacillus thuringiensis
beauveria bassiana
suspending agent
composition
spinosyns
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CN103875716B (en
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胡本进
胡飞
高同春
李昌春
周子燕
徐丽娜
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Institute of Plant Protection and Agricultural Products Quality Safety of AAAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The invention discloses a beauveria bassiana.bacillus thuringiensis.spinosyns composition, which is prepared by mixing bacillus thuringiensis oil suspension, beauveria bassiana dispersible oil suspension and spinosyns suspension at the weight ratio of 1:(1-2):(1-3), wherein the concentration of bacillus thuringiensis in the bacillus thuringiensis oil suspension is 7,000 to 9,000IU/microliter; the concentration of spinosyns in the spinosyns suspension is 50 to 70 grams/liter; the beauveria bassiana dispersible oil suspension is 30,000,000,000/gram beauveria bassiana dispersible oil suspension.

Description

The purposes of a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition and the control snout moth's larva of rice thereof
Technical field
The present invention relates to pesticide field, is exactly the purposes of a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition and the control snout moth's larva of rice thereof.
Background technology
Paddy rice is one of Three major grain crops of the mankind, and its yield and quality direct relation grain security and healthy diet.The snout moth's larva of rice is the most important Occurrence of south China paddy rice, comprises striped rice borer, pink rice borer, rice leaf roller etc., causes huge economic loss.At present, in Rice Production, mainly prevent and treat the snout moth's larva of rice by chemical control, although also played certain control action, the pesticide resistance of insect is not only brought out in the use of a large amount of chemical pesticides, has also brought serious environmental pollution and food-safety problem simultaneously.For addressing the above problem, we have carried out the field control effectiveness test of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition to the snout moth's larva of rice at home first.
Summary of the invention
The object of the present invention is to provide the purposes of a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition and the control snout moth's larva of rice thereof.
Above-mentioned purpose realizes by following scheme:
A kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, it is characterized in that: its be by bacillus thuringiensis,Bt oil-suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent is obtained by mixing according to the weight proportion of 1:1-2:1-3, in bacillus thuringiensis,Bt oil-suspending agent, the concentration of bacillus thuringiensis,Bt is 7000-9000IU/ microlitre, the concentration of multiple killing teichomycin suspending agent is 50-70 grams per liter, and it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
Described a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, is characterized in that: its amount of application is 100-160 milliliter/mu.
Described a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, is characterized in that: its formulation is oil-suspending agent.
The purposes of described beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, is characterized in that: it can be used for preventing and treating the snout moth's larva of rice.
The purposes of described beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, is characterized in that: it can be used for preventing and treating paddy rice rice leaf roller, rice-stem borer, paddy rice pink rice borer.
Beneficial effect of the present invention is: beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition is control snout moth's larva good medicament, and formulation can be oil-suspending agent etc., and its drug effect is higher than single dose and composite preventive effect between two, and three is composite has synergistic effect.
Embodiment mono-
1, materials and methods
1.1 situations experimental field
Test is located at great Ma administrative village, Tong Zha town, Hanshan County.The native field of the liver of being experimental field born in the year of horse.The liver field of being experimental field born in the year of horse, in soil, total nitrogen content is 3.25g/kg, available phosphorus, effectively potassium content is respectively 11.3mg/kg, 364.3mg/kg, pH value 6.2, content of organic matter 29.38g/kg.Cultivation condition uniformity, meets local agricultural practice.
1.2 subjects
Bore moth property snout moth's larva (mixed population of striped rice borer and pink rice borer)
1.3 reagent agent
8000IU/ microlitre bacillus thuringiensis,Bt oil-suspending agent 60 grams per liter multiple killing teichomycin suspending agents
A 30000000000/gram beauveria bassiana dispersibles oil-suspending agent 20% Rynaxypyr suspending agent
1.4 test method
1.4.1 test is processed
Figure BDA0000474431400000021
A dispenser on September 6 in 2013, when dispenser paddy rice in earing to blooming stage, now snout moth's larva of rice emergence period just.
1.4.2 investigation method
Investigation on September 29 (after medicine 23 days) preventive effect.Parallel great-jump-forward sampling, 50 clumps of rice of every community investigation, statistics dead ears number, calculates dead ears rate, preventive effect.
1.4.2.3 drug effect computational methods
Figure BDA0000474431400000031
Figure BDA0000474431400000032
2, results and analysis
Control paddy rice is bored moth property snout moth's larva effect comparison sheet
Figure BDA0000474431400000033
From table, result of the test shows, bacillus thuringiensis,Bt, beauveria bassiana, multiple killing teichomycin have good preventive effect to paddy rice brill moth property snout moth's larva, and single dose reaches respectively 45.73%, 65.84%, 67.42%; And compound proportion (1:1) preventive effect is respectively 52.62%, 70.58%, 75.87% between two; And bacillus thuringiensis,Bt, beauveria bassiana, multiple killing teichomycin three compound proportion (1:1:1) preventive effect are 78.08%, (1:1:2) preventive effect is 80.23%, (1:1:3) preventive effect is 83.35%; , higher than contrast medicament, single dose and composite preventive effect between two, three is composite has synergistic effect.
Conclusion: beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition is that control paddy rice is bored the good medicament of moth property snout moth's larva, and formulation can be oil-suspending agent.
Embodiment bis-
1, materials and methods
1.1 subjects
Rice leaf roller
1.2 reagent agent
8000IU/ microlitre bacillus thuringiensis,Bt oil-suspending agent 60 grams per liter multiple killing teichomycin suspending agents
A 30000000000/gram beauveria bassiana dispersibles oil-suspending agent 20% Rynaxypyr suspending agent
1.3 investigation method
14 days investigation preventive effects after medicine.Parallel jump method is investigated 5 points, and 5 clumps every, 25 clumps of rice of every community investigation, record every total number of sheets and leaf roll number, calculate preventive effect.
1.4 drug effect computational methods
Figure BDA0000474431400000042
2, results and analysis
Control paddy rice rice leaf roller effect comparison sheet
Figure BDA0000474431400000043
From table, result of the test shows, bacillus thuringiensis,Bt, beauveria bassiana, multiple killing teichomycin have good preventive effect to paddy rice rice leaf roller, and single dose reaches respectively 43.65%, 62.38%, 64.69%; And compound proportion (1:1) preventive effect is respectively 54.87%, 72.52%, 74.68% between two; And bacillus thuringiensis,Bt, beauveria bassiana, multiple killing teichomycin three compound proportion (1:1:1) preventive effect are 77.54%, (1:1:2) preventive effect is 78.53%, (1:1:3) preventive effect is 80.27%; , higher than contrast medicament, single dose and composite preventive effect between two, three is composite has synergistic effect.
Conclusion: beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition is the good medicament of control paddy rice rice leaf roller, and formulation can be oil-suspending agent.
Embodiment 3,
A kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, it is characterized in that: its be by bacillus thuringiensis,Bt oil-suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent is obtained by mixing according to the weight proportion of 1:2:1, in bacillus thuringiensis,Bt oil-suspending agent, the concentration of bacillus thuringiensis,Bt is 8000IU/ microlitre, the concentration of multiple killing teichomycin suspending agent is 60 grams per liters, and it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
Described a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, its amount of application is 160 milliliters/mu, its formulation is oil-suspending agent.
Its preventive effect of boring moth property snout moth's larva, rice leaf roller for paddy rice is 79.45% and 93.52%.
Embodiment 4,
A kind of composition of preventing and treating the snout moth's larva of rice, it is to dispersible oil-suspending agent, multiple killing teichomycin suspending agent and Chinese medical extract by bacillus thuringiensis,Bt oil-suspending agent, beauveria bassiana to be obtained by mixing according to the weight proportion of 1:1:1:0.2, in bacillus thuringiensis,Bt oil-suspending agent, the concentration of bacillus thuringiensis,Bt is 9000IU/ microlitre, the concentration of the multiple killing teichomycin of multiple killing teichomycin suspending agent is 60 grams per liters, and it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
Described Chinese medical extract is obtained by the raw material water extraction of following weight parts: morning glory 2, scholar chaste tree skin 5, tomato leaf 5, the root of langdu 2, huechys 3, datura 2, capsicum leaf 4.
Its amount of application is 100-160 milliliter/mu.
Its formulation is oil-suspending agent.It can be used for preventing and treating rice leaf roller, rice-stem borer, paddy rice pink rice borer.

Claims (4)

1. a beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition, it is characterized in that: its be by bacillus thuringiensis,Bt oil-suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent is obtained by mixing according to the weight proportion of 1:1-2:1-3, in bacillus thuringiensis,Bt oil-suspending agent, the concentration of bacillus thuringiensis,Bt is 7000-9000IU/ microlitre, the concentration of multiple killing teichomycin suspending agent is 50-70 grams per liter, and it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
2. a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition according to claim 1, is characterized in that: its amount of application is 100-160 milliliter/mu.
3. a kind of beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition according to claim 1, is characterized in that: its formulation is oil-suspending agent.
4. a purposes for beauveria bassiana bacillus thuringiensis,Bt multiple killing teichomycin composition as claimed in claim 1, is characterized in that: it can be used for preventing and treating the snout moth's larva of rice.
CN201410084227.9A 2014-03-07 2014-03-07 A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and the purposes of the preventing and treating snout moth's larva of rice thereof Active CN103875716B (en)

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CN106172504A (en) * 2016-06-29 2016-12-07 江苏邦盛生物科技有限责任公司 A kind of containing multiple killing teichomycin and the insecticides of Bacillus thuringiensis and application
CN106719846A (en) * 2016-11-22 2017-05-31 恒大园林集团有限公司 A kind of complex ecological agricultural chemicals and preparation method thereof
CN106942277A (en) * 2017-03-21 2017-07-14 山东礽健生物科技有限公司 A kind of biological pesticide and its production method for preventing and treating fragrant-flowered garlic maggot
CN113142245A (en) * 2021-01-05 2021-07-23 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram
CN113142244A (en) * 2021-01-05 2021-07-23 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106172504A (en) * 2016-06-29 2016-12-07 江苏邦盛生物科技有限责任公司 A kind of containing multiple killing teichomycin and the insecticides of Bacillus thuringiensis and application
CN106719846A (en) * 2016-11-22 2017-05-31 恒大园林集团有限公司 A kind of complex ecological agricultural chemicals and preparation method thereof
CN106942277A (en) * 2017-03-21 2017-07-14 山东礽健生物科技有限公司 A kind of biological pesticide and its production method for preventing and treating fragrant-flowered garlic maggot
CN113142245A (en) * 2021-01-05 2021-07-23 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram
CN113142244A (en) * 2021-01-05 2021-07-23 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram
CN113142244B (en) * 2021-01-05 2022-03-29 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram
CN113142245B (en) * 2021-01-05 2022-04-05 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram

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