CN103875716B - A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and the purposes of the preventing and treating snout moth's larva of rice thereof - Google Patents

A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and the purposes of the preventing and treating snout moth's larva of rice thereof Download PDF

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CN103875716B
CN103875716B CN201410084227.9A CN201410084227A CN103875716B CN 103875716 B CN103875716 B CN 103875716B CN 201410084227 A CN201410084227 A CN 201410084227A CN 103875716 B CN103875716 B CN 103875716B
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Prior art keywords
suspending agent
bacillus thuringiensis
beauveria bassiana
oil
multiple killing
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CN103875716A (en
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胡本进
胡飞
高同春
李昌春
周子燕
徐丽娜
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Institute of Plant Protection and Agricultural Products Quality Safety of AAAS
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Institute of Plant Protection and Agricultural Products Quality Safety of AAAS
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, its be dispersibled oil-suspending agent by Bacillus thuringiensis oil-suspending agent, beauveria bassiana, multiple killing teichomycin suspending agent is obtained by mixing according to the weight proportion of 1:1 2:1 3, in Bacillus thuringiensis oil-suspending agent, the concentration of Bacillus thuringiensis is 7000 9000IU/ microlitres, the concentration of the multiple killing teichomycin of multiple killing teichomycin suspending agent is 50 70 grams per liters, and it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.

Description

A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and anti-harness the river The purposes of rice borer
Technical field
The present invention relates to pesticide field, be exactly a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin combination Thing and the purposes of the preventing and treating snout moth's larva of rice thereof.
Background technology
Oryza sativa L. is one of Three major grain crops of the mankind, and its yield and quality direct relation grain security and diet is good for Health.The snout moth's larva of rice is the most important Occurrence of south China Oryza sativa L., including striped rice borer, pink rice borer, rice leaf roller etc., causes Huge economic loss.At present, in Rice Production, mainly prevent and treat the snout moth's larva of rice by chemical prevention, although also play Certain control action, but the use of a large amount of chemical pesticide not only induces the Drug resistance of insect, also brings serious ring simultaneously Environment pollution and food-safety problem.For solving the problems referred to above, we have carried out beauveria bassiana Su Yun gold bar first The endothal mould promotor composition field control effectiveness test to the snout moth's larva of rice.
Summary of the invention
It is an object of the invention to provide a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and prevent Harness the river the purposes of rice borer.
Above-mentioned purpose is realized by below scheme:
A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: it is by Su Yun gold bar Bacterium oil-suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent mixes according to the weight proportion of 1:1-2:1-3 And obtain, in Bacillus thuringiensis oil-suspending agent, the concentration of Bacillus thuringiensis is 7000-9000IU/ microlitre, multiple killing teichomycin suspending agent Concentration is 50-70 grams per liter, and it is that a 30000000000/gram beauveria bassiana dispersibles oily hanging that described beauveria bassiana dispersibles oil-suspending agent Floating agent.
Described a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: its amount of application For 100-160 milliliter/mu.
Described a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: its dosage form is Oil-suspending agent.
The purposes of described beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: it can use In the preventing and treating snout moth's larva of rice.
The purposes of described beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: it can use In preventing and treating Oryza sativa L. rice leaf roller, rice-stem borer, Rice ragged stunt virus.
The invention have the benefit that beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions is to prevent and treat snout moths larva relatively Good medicament, dosage form can be oil-suspending agent etc., and its drug effect is higher than single dose and preventive effect compounding two-by-two, and three is compounding has potentiation Effect.
Embodiment one
1, materials and methods
1.1 experimental field situations
Test is located at great Ma administrative village, Tong Zha town, Hanshan County.Experimental field it is born in the year of horse liver soil field.Experimental field it is born in the year of horse liver field, in soil Total nitrogen content is 3.25g/kg, and available phosphorus, effective potassium content are respectively 11.3mg/kg, 364.3mg/kg, pH value 6.2, organic matter Content 29.38g/kg.Cultivation condition uniformity, meets the agricultural practice of locality.
1.2 subjects
Bore moth property snout moths larva (striped rice borer and the mixed population of pink rice borer)
1.3 reagent agent
8000IU/ microlitre Bacillus thuringiensis oil-suspending agent 60 grams per liter multiple killing teichomycin suspending agent
A 30000000000/gram beauveria bassiana dispersibles oil-suspending agent 20% Rynaxypyr suspending agent
1.4 test method
1.4.1 test processes
JIUYUE in 2013 dispenser on the 6th, during dispenser, Oryza sativa L. is in and ears to blooming stage, and the snout moth's larva of rice occurs the most just Phase.
1.4.2 investigation method
JIUYUE 29 days (after medicine 23 days) investigation preventive effect.Parallel saltatory samples, 50 clumps of rice of every community investigation, adds up dead ears number, Calculate dead ears rate, preventive effect.
1.4.2.3 drug effect computational methods
2, result and analysis
Preventing and treating Oryza sativa L. bores moth property snout moths larva effectiveness comparison table
From table, result of the test shows, Oryza sativa L. is bored moth property snout moths larva by Bacillus thuringiensis, beauveria bassiana, multiple killing teichomycin to be had relatively Good preventive effect, single dose reaches 45.73%, 65.84%, 67.42% respectively;And two-by-two compound proportion (1:1) preventive effect be respectively 52.62%, 70.58%、75.87%;And Bacillus thuringiensis, beauveria bassiana, multiple killing teichomycin three's compound proportion (1:1:1) preventive effect are 78.08%, (1:1:2) preventive effect be 80.23%, (1:1:3) preventive effect be 83.35%;, higher than comparison medicament, single dose and compounding two-by-two Preventive effect, three is compounding has potentiation.
Conclusion: beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions is that preventing and treating Oryza sativa L. brill moth property snout moths larva is preferable Medicament, dosage form can be oil-suspending agent.
Embodiment two
1, materials and methods
1.1 subjects
Rice leaf roller
1.2 reagent agent
8000IU/ microlitre Bacillus thuringiensis oil-suspending agent 60 grams per liter multiple killing teichomycin suspending agent
A 30000000000/gram beauveria bassiana dispersibles oil-suspending agent 20% Rynaxypyr suspending agent
1.3 investigation method
14 days investigation preventive effects after medicine.Parallel jump method investigates 5 points, and 5 clumps every, 25 clumps of rice are investigated in every community, record every point Total number of sheets and leaf roll number, calculate preventive effect.
1.4 drug effect computational methods
2, result and analysis
Preventing and treating Oryza sativa L. rice leaf roller effectiveness comparison table
From table, result of the test shows, Oryza sativa L. rice leaf roller is had relatively by Bacillus thuringiensis, beauveria bassiana, multiple killing teichomycin Good preventive effect, single dose reaches 43.65%, 62.38%, 64.69% respectively;And two-by-two compound proportion (1:1) preventive effect be respectively 54.87%, 72.52%、74.68%;And Bacillus thuringiensis, beauveria bassiana, multiple killing teichomycin three's compound proportion (1:1:1) preventive effect are 77.54%, (1:1:2) preventive effect be 78.53%, (1:1:3) preventive effect be 80.27%;, higher than comparison medicament, single dose and compounding two-by-two Preventive effect, three is compounding has potentiation.
Conclusion: beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions is that preventing and treating Oryza sativa L. rice leaf roller is preferable Medicament, dosage form can be oil-suspending agent.
Embodiment 3,
A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: it is by Su Yun gold bar Bacterium oil-suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent according to 1:2:1 weight proportion mix and , in Bacillus thuringiensis oil-suspending agent, the concentration of Bacillus thuringiensis is 8000IU/ microlitre, and the concentration of multiple killing teichomycin suspending agent is 60 Grams per liter, it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
Described a kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, its amount of application is 160 milliliters/ Mu, its dosage form is oil-suspending agent.
It bores moth property snout moths larva for Oryza sativa L., the preventive effect of rice leaf roller is 79.45% and 93.52%.
Embodiment 4,
A kind of compositions preventing and treating the snout moth's larva of rice, it is to be dispersibled oil by Bacillus thuringiensis oil-suspending agent, beauveria bassiana Suspending agent, multiple killing teichomycin suspending agent and Chinese medicine extract are obtained by mixing according to the weight proportion of 1:1:1:0.2, Su Yun gold bar In bacterium oil-suspending agent, the concentration of Bacillus thuringiensis is 9000IU/ microlitre, and the concentration of the multiple killing teichomycin of multiple killing teichomycin suspending agent is 60 Grams per liter, it is that a 30000000000/gram beauveria bassiana dispersibles oil-suspending agent that described beauveria bassiana dispersibles oil-suspending agent.
Described Chinese medicine extract is carried by the raw water of following weight parts and obtains: morning glory 2, scholar's chaste tree skin 5, tomato leaf 5, The Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae) 2, Huechys sanguinea De Geer. 3, Flos Daturae 2, Folium Capsici 4.
Its amount of application is 100-160 milliliter/mu.
Its dosage form is oil-suspending agent.It can be used for preventing and treating rice leaf roller, rice-stem borer, Rice ragged stunt virus.

Claims (3)

1. a beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions, it is characterised in that: it is by Bacillus thuringiensis oil Suspending agent, beauveria bassiana dispersible oil-suspending agent, multiple killing teichomycin suspending agent is joined according to the weight of 1:1:1,1:1:2 or 1:1:3 Ratio is obtained by mixing, and in Bacillus thuringiensis oil-suspending agent, the concentration of Bacillus thuringiensis is 7000-9000IU/ microlitre, and multiple killing teichomycin hangs The concentration of floating agent is 50-70 grams per liter, and it is that 30,000,000,000/gram beauveria bassiana can divide that described beauveria bassiana dispersibles oil-suspending agent Dissipate oil-suspending agent.
A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions the most according to claim 1, it is characterised in that: Its dosage form is oil-suspending agent.
3. the beauveria bassiana Bacillus thuringiensis multiple killing teichomycin composition for preventing and controlling snout moth's larva of rice as claimed in claim 1 Purposes, it is characterised in that: amount of application is 100-160 milliliter/mu.
CN201410084227.9A 2014-03-07 2014-03-07 A kind of beauveria bassiana Bacillus thuringiensis multiple killing teichomycin compositions and the purposes of the preventing and treating snout moth's larva of rice thereof Active CN103875716B (en)

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CN106172504A (en) * 2016-06-29 2016-12-07 江苏邦盛生物科技有限责任公司 A kind of containing multiple killing teichomycin and the insecticides of Bacillus thuringiensis and application
CN106719846A (en) * 2016-11-22 2017-05-31 恒大园林集团有限公司 A kind of complex ecological agricultural chemicals and preparation method thereof
CN106942277A (en) * 2017-03-21 2017-07-14 山东礽健生物科技有限公司 A kind of biological pesticide and its production method for preventing and treating fragrant-flowered garlic maggot
CN113142245B (en) * 2021-01-05 2022-04-05 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB038 and spinetoram
CN113142244B (en) * 2021-01-05 2022-03-29 华南农业大学 Synergistic prevention and treatment of common thrips by Beauveria bassiana SB063 and spinetoram

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CN1168387C (en) * 2003-03-05 2004-09-29 湖北省生物农药工程研究中心 Bacillus thuringiensis oil suspension concentrate pesticide
BG1238U1 (en) * 2009-06-09 2009-11-30 Георги Величков Composition used to improve the effect of vegetation herbicides and insecticides
CN102172250A (en) * 2011-03-24 2011-09-07 中国农业科学院农业环境与可持续发展研究所 Preparation method and application of eauveria-spinosad suspending agent
CN102687731B (en) * 2012-06-20 2013-11-13 东北林业大学 Wettable powder of bacillus thuringiensis and beauveria bassiana

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